Tag: hiPSCs

Tarawneh R, Holtzman DM. The clinical problem of symptomatic Alzheimer disease and mild cognitive impairment. Cold Spring Harb Perspect Med. 2012;2(5): a006148. Article  PubMed  PubMed Central  Google Scholar  van der Flier WM, Scheltens P. Epidemiology and risk factors of dementia. J Neurol Neurosurg Psychiatry. 2005;76(suppl 5):v2-7. Article  PubMed  PubMed Central …

CRISPR and Cas Genes Market Size, Share & Trends Analysis Report by Product Type (Vector-based Cas and DNA-free Cas), by Service Type (Cell Line Engineering, gRNA design, Microbial Gene Editing and DNA Synthesis), by Application (Genome Engineering, Disease Models, Functional Genomics and Other) and by End Users (Biotechnology Companies &…

Background: Centrosomal protein 83 (CEP83) is a component of the distal appendages proteins (DAPs) of centrioles, which is necessary for the assembly of primary cilia. Primary cilia play an essential role in early embryonic development, organ development, and tissue homeostasis. Loss of many ciliary proteins in mice models results in…

Rowe RG, Daley GQ. Induced pluripotent stem cells in disease modelling and drug discovery. Nat Rev Genet. 2019;20:377–88. Article  CAS  PubMed  PubMed Central  Google Scholar  Li L, Papadopoulos V. Advances in stem cell research for the treatment of primary hypogonadism. Nat Rev Urol. 2021;18:487–507. Article  CAS  PubMed  Google Scholar  Lawrence…

A recent study published in Genome Biology has shed light on the profound effects of a loss-of-function mutation in the oxidation resistance 1 (OXR1) gene on neurodevelopment and cellular functions in the human brain. While OXR1 is known for its crucial role in oxidative stress resistance, the specific mechanisms and…

hiPSC cultures and cardiomyocyte differentiation The hiPSC lines CBRCULi001-A54 and CBRCULi008-A55 were generated from a 44-year-old male and 75-year-old female control lymphoblastoids, respectively, and they were reprogramed at the LOEX core facility (Quebec City, QC, Canada). All the work with hiPSCs were approved by CIUSSS de la Capitale-Nationale ethics committee (Project…

Researchers at the University of Cambridge have made an important breakthrough in understanding the underlying causes of small vessel disease (SVD) in the brain, a condition that leads to vascular dementia and stroke. They have identified matrix metalloproteinases (MMPs) as a possible drug target to prevent the leakage of blood…

Scientists led by a team at the University of Cambridge used human induced pluripotent stem cells (hIPSCs) derived from patients with a genetic mutation that increases the risk of cerebral small vessel disease (SVD) to grow small blood vessel-like models in the lab. The team used these models to show…

If you have diabetes, the cells in the pancreas are unable to produce insulin, which is needed by the body to regulate blood sugar. Currently, efforts are being made in labs worldwide to create insulin-producing cells from human induced Pluripotent Stem Cells (hiPSCs) that can replace insulin supplements. One of…

Abstract Pathogenic variants in the GNAO1 gene, encoding the alpha subunit of an inhibitory heterotrimeric guanine nucleotide-binding protein (Go) highly expressed in the mammalian brain, have been linked to encephalopathy characterized by different combinations of neurological symptoms, including developmental delay, hypotonia, epilepsy and hyperkinetic movement disorder with life-threatening paroxysmal exacerbations….

  Human induced pluripotent stem cells (hiPSCs) hold great promise for treating many currently untreatable diseases. Scientists can turn them into brain, liver, heart and other types of cells that could mend injured or unhealthy ones. However, the processes for culturing and differentiating hiPSCs into other kinds of cells take…

In hiPSCs, researchers developed a strategy to prevent epigenetic aberrations caused by reprogramming. Human embryonic stem cells (ESCs), derived from the inner cell mass of the blastocyst before its attachment to the uterus, can differentiate into multiple cell types. Due to the ethical debate surrounding their use in research, scientists…

Global CRISPR and Cas genes market is anticipated to grow at a CAGR of 13.5% during the forecast period (2023-2030). The technological advancements in CRISPR & Cas gene systems have contemporarily revolutionized the area of cancer research and therapeutics. As the prevalence of genetic diseases and complex medical conditions continues…

Join us for an informative webinar on creating induced pluripotent stem cells (iPSCs), which sheds light on the strengths of the mRNA technique compared to other methods. Discover the new Miltenyi Biotec mRNA reprogramming workflow and its myriad of benefits including enhanced efficiency, safety, and reproducibility in a streamlined process…

Abstract Epigenetic variation in the X chromosome inactivation (XCI) of human induced pluripotent stem cells (hiPSCs) can impact their ability to accurately model biological sex biases. However, the gene-wise landscape of escape from XCI remains unresolved in female hiPSCs. To characterize the patterns of escape from inactivation, we performed a…

Human induced pluripotent stem cells (hiPSCs) are promising in regenerative medicine. However, the pluripotent stem cells (PSCs) may form clumps of cancerous tissue, which is a major safety concern in PSCs therapies. Rapamycin is a safe and widely used immunosuppressive pharmaceutical that acts through heterodimerization of the FKBP12 and FRB…

Begin with human induced pluripotent stem cell or hiPSC suspension in media. Add a pair of transcription activator-like effector nuclease or TALEN-encoding plasmids. Supplement with a donor plasmid bearing a fluorescent protein gene, coupled to an antibiotic resistance gene, with adjacent homology arms for specific alignment to target loci. Electroporate…

[Source] Researchers at the University of Oxford have developed a groundbreaking technique for 3D printing human stem cells to create an engineered tissue that mimics the structure of the cerebral cortex. How they did it: Using human induced pluripotent stem cells (hiPSCs) — which have the potential to generate most…

Clock.bio, a sister company of bit.bio, is dedicated to extending and improving the quality of life by reversing the harmful effects of time on our cells. Founded by Mark Kotter, who also serves as the CEO of bit.bio, Clock.bio harnesses the regenerative capabilities of human pluripotent stem cells (hiPSCs) to…

By Gail Dutton Although similar, the epigenomes of human induced pluripotent stem cells (hiPSCs) differ from those of the human embryonic stem cells (hESCs) they aim to replicate. These differences include epigenetic memory of their original specialized state and reprogramming aberrations that affect the cells’ functions, adding another challenge for…

Image: The 3D-printed 2-layer cerebral cortical tissue was implanted into a mouse brain slice (Photo courtesy of University of Oxford) Brain injuries often lead to severe damage to the cerebral cortex, the brain’s outer layer, which impairs cognition, movement, and communication. For instance, around 70 million people worldwide are affected…

In a scientific first, researchers at theUniversity of Oxford have 3D printed stem cells that can mimic the architecture of the cerebral cortex, the human brain’s outer layer. The technique could potentially be used to treat brain injuries. Such injuries typically cause significant damage to the cerebral cortex, leading to…

A new 3D printer breakthrough could offer hope to the millions worldwide who live with brain injuries, researchers have claimed. A team at the University of Oxford demonstrated for the first time that brain cells can be 3D-printed to “mimic the architecture of the cerebral cortex”. They fabricated a two-layered…

Oxford University researchers have used human stem cells to 3D print tissue that mimics the cerebral cortex and shows promise for treating brain injuries. Described in Nature Communications, the work ultimately saw the Oxford team implanting the 3D printed structures into mouse brain slices. According to the researchers, the…

Researchers have produced an engineered tissue representing a simplified cerebral cortex by 3D printing human stem cells. When implanted into mouse brain slices, the structures became integrated with the host tissue. The breakthrough technique developed by University of Oxford researchers could one day provide tailored repairs for those who suffer…

OXFORD, United Kingdom — Scientists from the University of Oxford have developed an innovative 3D-printing technique that might one day revolutionize treatments for brain injuries. This technique involves creating neural cells that emulate the structure of the cerebral cortex, the brain’s outer layer. Brain injuries, resulting from trauma, strokes, or…

Researchers at the University of Oxford have unveiled a pioneering method that has the potential to offer customized solutions for individuals affected by brain injuries. (Photo : FRED TANNEAU/AFP via Getty Images)A picture of a human brain taken by a positron emission tomography scanner, also called PET scan, is seen…

Background: In vitro production of hematopoietic stem/progenitor cells (HSPCs) from human-induced pluripotent stem cells (hiPSCs) provides opportunities for fundamental research, disease modeling, and large-scale production of HLA-matched HSPCs for therapeutic applications. However, a comprehensive understanding of the signaling mechanisms that regulate human hematopoiesis is needed to develop a more effective…

Culture of human-induced pluripotent stem cells (hiPSCs) Human iPSC lines were cultured in Matrigel-coated plates in the Nutristem medium (Corning, USA). Cells were passaged every 5 days by treating with Versene (Thermo Fisher Scientific, USA) for 3–5 min, transferred to freshly prepared Matrigel-coated plates, and cultured under hypoxic conditions with 5% CO2…

Human derived induced pluripotent stem cells (hiPSCs) have revolutionised research and are increasingly used for toxicology screening and disease modelling. Early detection of neurotoxicity induced by potential new therapies is a major challenge, and hiPSC-neuronal cells may provide a solution. These cells demonstrate considerable promise for uncovering drug-induced perturbations to…

Generation and characterisation of patient-specific hiPSCs and hiPSC-CMs hiPSC lines were established from two TOF-DG patients, two TOF-ND patients, and two healthy controls with pluripotency markers and germ layer markers verified (Supplementary Figs. 1 and 2). Whole genome sequencing confirmed, respectively, the presence and the absence of 22q11.2 deletion in the…

In the brain of healthy subjects, the neuronal activity is kept and controlled by a precise and well-defined balance between excitation (E) and inhibition (I) (Mossink et al 2022), defined as ‘E/I balance’. Since GABA is the main inhibitory neurotransmitter of the central nervous system (Leinekugel et al 1999), it…

While lingering memories can represent unwelcome guests, destroying them with the power of dynamite may seem like overkill! However, a volatile new study now describes TNT or transient-naïve-treatment as a simple means of ridding human induced pluripotent stem cells (hiPSC) of their epigenetic memories and improving their therapeutic potential. An…

Dermal papilla cells (DPCs) play roles in key functions of the epidermis such as hair generation. The use of human induced pluripotent cells (hiPSCs) makes it possible to obtain DP-like cells and study the molecular mechanisms of DPC development during embryogenesis. In this work, we studied the phenotypic trajectory of…

Biotech on $4m mission to extend healthspan by 20 years based on biomarkers of aging in Phase 3 trial by the end of this decade. Clock.bio, a longevity biotech developing novel regenerative medicines leveraging the natural ability of human pluripotent stem cells to prevent and treat age-related diseases, today announced…

Animal studies for evaluating the embryotoxicity of potential therapeutics and environmental factors are expensive and time-consuming. Additionally, these studies may not be directly applicable to humans due to species differences. In order to address these challenges, researchers conducted a study to identify a gene signature associated with the process of…

Directed differentiation of hiPSCs (SBAD2) towards cardiomyocytes after exposure to teratogens and non-teratogens To study the early events of the process of cardiomyogenesis, we used a cell monolayer-based directed hiPSC differentiation protocol, designated as the UKK2 cardiotoxicity test (UKK2-CTT), which is based on the sequential activation and inhibition of Wnt/β-catenin…

Animal studies used for evaluating the embryotoxicity of drugs and environmental factors are often costly, time-consuming, and not directly applicable to humans due to species differences. In this study, researchers aimed to replicate the process of cardiomyogenesis (formation of heart muscle cells) using human induced pluripotent stem cells (hiPSCs) to…

Transcription of ribosomal RNA (rRNA) by RNA Polymerase (Pol) I in the nucleolus is necessary for ribosome biogenesis, which is intimately tied to cell growth and proliferation. Perturbation of ribosome biogenesis results in tissue specific disorders termed ribosomopathies in association with alterations in nucleolar structure. However, how rRNA transcription and…

Title:Quest for alternate personalized clinical source of MSCs: Advancing towards hiPSCs derived iMSCs Volume: 11 Issue: 2 Author(s): Vikram Sabapathy and Sanjay Kumar Affiliation: Keywords: Human induced pluripotent stem cells (hiPSCs), mesenchymal stem cells (MSCs), mesenchymal stromal cells, stromal cells, iMSCs.Human induced pluripotent stem cells (hiPSCs), mesenchymal stem cells…

Researchers have achieved a significant breakthrough in the field of ocular research by successfully transplanting human microglia cells into the retina of mice. This innovative experiment, published in eLife, has unveiled the potential of microglial replacement therapy in the treatment of incurable eye diseases affecting the retina and central nervous…

image: The Texas Heart Institute recently received a five-year, $2 million grant from the National Heart, Lung, and Blood Institute of the National Institutes of Health to advance the technology supporting development of transplantable bioartificial hearts. The project, funded by a Stephen I. Katz Early Stage Investigator Research Project Grant, will…

Researchers have successfully transplanted human microglia cells into mouse retina to create a model that could be used to test new treatments for incurable eye diseases. The research, published today as a Reviewed Preprint in eLife, is described by the editors as an important study with solid data demonstrating the…

Researchers report that they have successfully transplanted human microglia cells into mouse retina to create a model that could be used to test new treatments for incurable eye diseases. The findings are published in eLife in the article titled, “Human iPSC-derived Microglia Cells Integrated into Mouse Retina and Recapitulated Features…

Image: The miniature human heart model could potentially transform drug testing and cardiovascular research (Photo courtesy of Tissue Dynamics) Cardiovascular ailments continue to be the primary cause of death worldwide. Now, a new multi-chambered, self-regulating miniature heart model offers a promising solution for improving survival and patient outcomes. In a…

Tiny, Multichambered Human Heart Model Developed by Hebrew University Researchers Could Transform Cardiovascular Research and Drug Evaluation   JERUSALEM, August 9, 2023 – A team of researchers, led by the Hebrew University of Jerusalem (HU), has developed a tiny human heart model that could potentially transform cardiovascular research and animal-free drug…

Newswise — A remarkable breakthrough, a collaborative team of researchers, led by Professor Yaakov Nahmias from The Hebrew University of Jerusalem, Technion-Israel Institute of Technology, and Tissue Dynamics Ltd., has unveiled a miniature human heart model that could potentially transform drug testing and cardiovascular research. This study, published in Nature Biomedical Engineering, introduces…

Authors: Louise C Laurent, Igor Ulitsky, Ileana Slavin, Ha Tran, Andrew Schork, Robert Morey, Candace Lynch, Julie V Harness, Sunray Lee, Maria J Barrero, Sherman Ku, Marina Martynova, Ruslan Semechkin, Vasiliy Galat, Joel Gottesfeld, Juan Carlos Izpisua Belmonte, Chuck Murry, Hans S Keirstead, Hyun-Sook Park, Uli Schmidt, Andrew L Laslett,…

To begin, take a flask of cultured fibroblast cells, coat a 10-centimeter cell culture plate suitable for hiPSC culture with four milliliters of cold coating solution per plate, and incubate the plate for one hour at 37 degrees Celsius. Afterward, remove the media from the fibroblasts and wash them with…

Transcript To begin, take the plate of electroporated fibroblasts and observe the formation of hiPSC colonies under a 10x to 20x objective phase contrast microscope. The hiPSC colonies with 300 micrometer diameter, distinct borders, and a high nuclei to body ratio are suitable for picking. Before picking, coat the wells…

Human induced pluripotent stem cells successfully differentiated into keratinocyte. Retinoic acid (RA) and BMP-4 were used to induce differentiation of hiPSCs into keratinocyte. The images as immunofluorescence staining of keratinocyte marker proteins K14, involucrin and loricrin at 14 d of induced differentiation, scale bar: 25 µm. Credit: World Journal of…

BACKGROUND Current evidence shows that human induced pluripotent stem cells (hiPSCs) can effectively differentiate into keratinocytes (KCs), but its effect on skin burn healing has not been reported. AIM To observe the effects of hiPSCs-derived KCs transplantation on skin burn healing in mice and to preliminarily reveal the underlying mechanisms….

FIGURE 1 Generation of hiPSC-derived ameloblast organoids. (A) Schematic overview of the procedures for generating… FIGURE 1 Generation of hiPSC-derived ameloblast organoids. (A) Schematic overview of the procedures for generating hiPSC-derived organoids. (B) Between days 0 and 4, hiPSCs were cultured in StemFlex medium using a U-bottom plate to form…

Research ethics for donor tissues All heart tissue samples were obtained from transplant donors after Research Ethics Committee approval and written informed consent from donor families as previously described2. The following ethics approvals for donors of additional heart tissue were obtained: D8 and A61 (REC reference 15/EE/0152, East of England…

. 2023 Jun 15;14:1141756. doi: 10.3389/fgene.2023.1141756. eCollection 2023. Yinge Jin  1 , Tao Chen  1 , Wei Zheng  1 , Jiahui Xi  1 , Yin Zi  1 , Jinling Wang  1 , Yue Chi  1 , Min Chen  1 , Qingjian Zou  1 , Chengcheng Tang  1 , Liangxue Lai  1   2 , Xiaoqing Zhou  1 Affiliations Expand Affiliations 1…

Dilated cardiomyopathy (DCM) is a primary myocardial disease, leading to heart failure and excessive risk of sudden cardiac death with rather poorly understood pathophysiology. In 2015, Parvari’s group identified a recessive mutation in the autophagy regulator, PLEKHM2 gene, in a family with severe recessive DCM and left ventricular non-compaction (LVNC)….

Abstract:  Duchenne Muscular Dystrophy (DMD) is a disorder caused by loss-of-function mutations in dystrophin, a critical protein that maintains muscle fiber integrity. Our lab discovered that dystrophin-deficient skeletal muscle stem cells, also known as satellite cells, cannot generate enough myogenic progenitors for proper muscle regeneration. Previously, we demonstrated that WNT7A,…

Topics/Call fo Papers The Nobel prize winning discovery of human induced pluripotent stem cells (hiPSCs) facilitated a new era of disease modeling in vitro. This event inspired clinicians and scientists alike to use those cells for regenerative medicine, or for modeling of genetic diseases, to gain further insights into disease mechanisms on a…

doi: 10.1016/j.exger.2023.112243. Online ahead of print. Affiliations Expand Affiliations 1 Key Laboratory of Neurological Function and Health & Department of Human Anatomy, School of Basic Medical Sciences, Guangzhou Medical University, Guangzhou, Guangdong 511436, China. 2 Department of Pediatrics, University of California-Irvine, Irvine, CA 92697, USA. 3 Key Laboratory of Neurological…

Cell Culture Harvest Volume or Buffer Volume January 24th 2019, Union City, CA. Sepragen Corporation announced today it had successfully completed research using a grant from the National Science Foundation to demonstrate the feasibility of using a “wicking” matrix bioreactor (WMB) for expansion of hiPSCs for tissue engineering and regenerative…

One of the biggest challenges facing scientists today is the need for in vitro cell models that accurately represent the complexity of human diseases. In this workshop, you will hear from neuroscience experts as they share their practical experiences in using human iPSC-derived cells to effectively model neurodegeneration, neuroimmunology, and…

1Yonsei University College of Dentistry, Republic of Korea The final, formatted version of the article will be published soon. Notify me Receive an email when it is updated You just subscribed to receive the final version of the article Tooth formation relies on…

PRESS RELEASE Published June 14, 2023 Newark, New Castle, USA: The “Organoids Market” provides a value chain analysis of revenue for the anticipated period from 2022 to 2030. The report will include a full and comprehensive analysis of the business operations of all market leaders in this industry, as well…

The featured speakers used a stirred suspension bioreactor system to create an optimal environment for cell growth and differentiation. TORONTO (PRWEB) June 12, 2023 The Nobel prize winning discovery of human induced pluripotent stem cells (hiPSCs) facilitated a new era of disease modeling in vitro. This event inspired clinicians…

The generation of a homogeneous population of microglia from human induced pluripotent stem cells (hiPSCs) is crucial to modeling neurological disorders, as well as the carrying out of drug screening and toxicity testing. Here, we provide a stepwise protocol for the simple, robust, and efficient differentiation of hiPSCs into microglia-like…

Credit: Marcin Klapczynski / iStock A “village” approach that cultures cells from hundreds of people simultaneously could provide new insights into how genetics affect disease and drug treatment response, researchers say. The village system involves culturing and differentiating cells from multiple donor individuals in a single dish, then analyzing using…

Duchenne muscular dystrophy (DMD) is an X-linked progressive muscle degenerative disease caused by mutations in the dystrophin gene, resulting in death by the end of the third decade of life at the latest. A key aspect of the DMD clinical phenotype is dilated cardiomyopathy, affecting virtually all patients by the…

Xiaoqing Zhou1*, Yinge Jin1, Tao Chen1, Wei Zheng1, Jiahui Xi1, Yin Zi1, Jinling Wang1, Yue Chi1, Min Chen1, Qingjian Zou1, Chengcheng Tang1* and Liangxue Lai2* 1School of Biotechnology and Health, Wuyi University, China 2Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences (CAS), China …

A team of Stanford researchers has developed a novel method for quickly and efficiently generating human induced pluripotent stem cells (hiPSCs) using human adipose stem cells (hASCs) as the starting population. Using this technique, the whole reprogramming process was reduced to ~18 days with approximately 20-fold increase in efficiency compared…

From a database of more than 200,000 high-resolution, three-dimensional images of human induced pluripotent stem cells, researchers have devised a model to quantify cell shape and internal organization. Susanne Rafelski, Deputy Director of the Allen Institute for Cell Science, revealed details of their study to Drug Target Review. A recently…

hiPSC cell culture and differentiation hiPSCs were maintained on 1:40 matrigel (Corning, #354277) coated dishes in supplemented mTeSR-1 medium (StemCell Technologies, #85850) with 500 U ml−1 penicillin and 500 mg ml−1 streptomycin (Gibco, #15140122). For the differentiation of cortical neurons the protocol described previously21 was followed with slight modifications. Briefly, hiPSC colonies were seeded…

OSAKA, Japan – May 11, 2023 – FUJIFILM Wako Pure Chemical Corporation (President: Koichi Yoshida) is pleased to announce the launch of its Good Manufacturing Practice （GMP）-compliant ROCK inhibitor*1 “Y-27632 for industrial cell culture applications. Y-27632 offered by FUJIFILM Wako Pure Chemical Corporation is compliant with the ICH-Q7 GMP for…

doi: 10.1093/infdis/jiad141. Online ahead of print. Affiliations Expand Affiliations 1 Department of Environmental Health and Engineering, Johns Hopkins University School of Public Health, 615 North Wolfe Street, Baltimore, Maryland 21205, USA. 2 Department of Medicine/Division of Infectious Diseases, Johns Hopkins University School of Medicine, 1550 Orleans Street, CRB 2, Baltimore,…

Feigin VL, Stark BA, Johnson CO, Roth GA, Bisignano C, Abady GG, et al. Global, regional, and national burden of stroke and its risk factors, 1990–2019: A systematic analysis for the Global Burden of Disease Study 2019. Lancet Neurol. 2021;20:1–26. Article  Google Scholar  Wolters FJ, Arfan IM. Epidemiology of Vascular…

In this chapter, we will first introduce the causes of familial hypercholesterolemia disease, available treatments, and cell and animal models. As they keep the mutations inside their genome after reprogramming, patient’s specific human-induced pluripotent stem cells (hiPSCs) seem to be the most relevant cells to model familial hypercholesterolemia. In a…

Materials The materials used for cell cultures and characterization are listed in Additional file 1: Table S1. Cell sources After getting informed consent, PB was drawn from three healthy O, Rh D-positive donors. CB was collected from three healthy newborn babies at the Department of Obstetrics and Gynecology at Severance…

PRESS RELEASE Published April 19, 2023 Organoids Market 2021 by Technology, Manufacturers, Regions, Type, and Application, Forecast guide to light the excellent analysis on the market synopsis. The report offers a primary focus on important factors in the global Organoids industry. The report includes perceptive information about gross revenue, cost,…

To promote the differentiation of human-induced pluripotent stem cells (hiPSCs) into myocardium through a standard chemically defined and small-molecule-based induction protocol (CDM3), and preliminarily prepare myocardial patches that provide experimental data and theoretical support for further maturation through other in vitro experiments and safety studies in vivo. After resuscitation, culture,…

Cell culture We cultured mESCs in t2iL medium containing Dulbecco’s modified eagle medium (DMEM, Nacalai Tesque), 2 mM Glutamax (Nacalai Tesque), 1× non-essential amino acids (Nacalai Tesque), 1 mM sodium pyruvate (Nacalai Tesque), 100 U ml−1 penicillin, 100 μg ml−1 streptomycin (P/S) (Nacalai Tesque), 0.1 mM 2-mercaptoethanol (Sigma) and 15% fetal bovine serum (FBS) (Gibco), supplemented with…

Off-the-shelf iPSC lines StemRNA human induced pluripotent stem cells (hiPSCs) provide immediate access to the state-of-the-art Stemgent StemRNA 3rd Gen Reprogramming technology. These cells are ideal for validating the StemRNA 3rd Gen Technology with control cells prior to investing in reprogramming to develop patient-derived cell lines. Our StemRNA hiPSCs are…

HiPSCs were maintained in mTeSR1 medium. When they had reached an optimum density of 3×104/cm2 they were switched to unconditioned (UC) medium for 6 days, followed by serum free endothelial cell (EC) medium containing B27, human basic fibroblast growth factor (hbFGF) and retinoic acid (RA) for 2 days. Strongly adherent…

Abstract In early human development, gastrulation is tightly associated with lineage specification. The interplay between mechanical forces and biochemical signals during these processes is poorly understood. Here, we dissect the effects of biochemical cues and physical confinement on a 3D in vitro model of gastrulation that uses spheroids formed from…

The three-dimensional (3D) retinal organoids (ROs) derived from human induced pluripotent stem cells (hiPSCs), mimicking the growth and development of the human retina, is a promising model for investigating inherited retinal diseases (IRD) in vitro. However, the efficient generation of homogenous ROs remains a challenge. Here we introduce a novel…

Summary Skeletal muscle regeneration is highly impaired in patients suffering from skeletal muscle disorders like muscular dystrophies, volumetric muscle loss or sarcopenia. Clinically relevant in-vitro skeletal muscle models are needed to better understand these disorders and develop personalized therapeutic strategies. Closely mimicking the developmental myogenesis and the anisotropic organization of…

doi: 10.1002/mabi.202200460. Online ahead of print. Affiliations Expand Affiliations 1 Department of Materials Science and Engineering, University of Washington, Seattle, Washington, 98195, USA. 2 Department of Neurological Surgery, University of Washington, Seattle, Washington, 98195, USA. Item in Clipboard Yuanzhang Tang et al. Macromol Biosci. 2023. Show details Display options Display options…

doi: 10.7554/eLife.83291. Merrick D Pierson Smela #  1   2 , Christian C Kramme #  1   2 , Patrick R J Fortuna  1   2 , Jessica L Adams  1   2 , Rui Su  1   2 , Edward Dong  1   2 , Mutsumi Kobayashi  3 , Garyk Brixi  1   2   4   5…

Scientists at the Wyss Institute for Biologically Inspired Engineering at Harvard University, Harvard Medical School (HMS), and Duke University, in collaboration with Gameto, report that they have created a living, fully human ovarian organoid that supports egg cell maturation, develops follicles, and secretes sex hormones. This “ovaroid” model enables the…

1. Research on the Mechanism of Parkinson’s Disease Construction of PARK2-KO-iPSC Model for the Study of Parkinson’s Disease Parkinson’s disease (PD) is an incurable neurodegenerative disease characterized by progressive loss of midbrain dopaminergetic neurons in the substantia nigra pars compacta (SNc), which leads to the subsequent depletion of the striatum dopamine. The mechanisms of…

1. Introduction Achromatopsia (ACHM) is an inherited retinal dystrophy (IRD) affecting approximately 1 in every 30,000 individuals [1,2]. It is an autosomal-recessive disorder associated with a loss of cone photoreceptor function [1,2,3,4]. ACHM usually shows an early onset presenting pendular nystagmus, poor visual acuity, lack of color vision, and photophobia…

Under the direction of Dr. Camila Hochman-Mendez in the Regenerative Medicine Research Department at The Texas Heart Institute, researchers are working to further refine the science of creating bioengineered organs and tissues. As part of this greater goal, they recently discovered a novel method to expand pluripotent stem cell populations…

Abstract Standard transgenic cell line generation requires screening 100-1000s of colonies to isolate correctly edited cells. We describe CRISPRa On-Target Editing Retrieval (CRaTER) which enriches for cells with on-target knock-in of a cDNA-fluorescent reporter transgene by transient activation of the targeted locus followed by flow sorting to recover edited cells….

Review doi: 10.3389/fcell.2022.1107881. eCollection 2022. Affiliations Expand Affiliations 1 Cellular Reprogramming Unit, Fondazione IRCCS Casa Sollievo della Sofferenza, San Giovanni Rotondo, Italy. 2 Department of Biotechnology and Biosciences, University of Milano-Bicocca, Milan, Italy. Free PMC article Item in Clipboard Review Angela Maria Giada Giovenale et al. Front Cell Dev Biol. 2023….

doi: 10.3389/fcell.2022.1071243. eCollection 2022. Affiliations Expand Affiliations 1 Department of Anatomy, Biochemistry and Physiology, Honolulu, HI, United States. 2 Center for Cardiovascular Research, Honolulu, HI, United States. 3 Department of Medicine, Honolulu, HI, United States. 4 Department of Quantitative Health Sciences, Honolulu, HI, United States. 5 Genomics and Bioinformatics Shared…

Cultivating quality cells is a key requirement for innovative approaches in regenerative medicine. Stirred-tank bioreactors are promising cultivation systems for the cultivation and differentiation of human induced pluripotent stem cells (hiPSCs), as they have the capacity to produce high cell numbers, allowing scale-up, and cutting-edge opportunities for improving the control…

In a recent study posted to the bioRxiv* preprint server, researchers developed a three-dimensional (3D) in vitro tissue model to assess the effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the vascular endothelial cells. Study: SARS-CoV-2 spike protein induces endothelial dysfunction in 3D engineered vascular networks. Image Credit:…

Gebhardt, R. & Matz-Soja, M. Liver zonation: Novel aspects of its regulation and its impact on homeostasis. World J. Gastroenterol. 20, 8491–8504 (2014). PubMed  PubMed Central  Article  Google Scholar  Summar, M. L. et al. The incidence of urea cycle disorders. Mol. Genet Metab. 110, 179–180 (2013). CAS  PubMed  PubMed Central …

Patients and controls The patient 1 was 38 years old and consulted for infertility after he and his partner had been trying to conceive for 2 years. The patient was the first child of unrelated parents, and he had four brothers and five sisters whose fertility status could not be determined…

Cost   *Course fee £525  This is a residential course and the fee includes all accommodation and meals. *The course fee is subsidised by Wellcome Genome Campus Advanced Courses and Scientific Conferences and applies to non-commercial applicants. Please contact us for the commercial fee. BursariesLimited bursaries are available (up to 50%…

Rivetti di Val Cervo P et al. Nature Reviews. Neurology. 2021 06; 17(6):381-392 doi.org/10.1038/s41582-021-00465-0PMID: 33658662 Show Details AbstractAuthors Human induced pluripotent stem cells (hiPSCs) were first generated in 2007, but the full translational potential of this valuable tool has yet to be realized. The potential applications of hiPSCs are especially…

Salary per annum: c.£33,900 Contract Length: 2 years Here at the world-famous and internationally respected Wellcome Sanger Institute, we have an exciting opportunity for a postdoctoral researcher to perform high throughput screening for transcription factor-driven differentiation in human pluripotent stem cells (hiPSCs) in order to create better in vitro differentiated cells that more faithfully…