Tag: hiPSCs
Improving Stem Cell Cultivation in Bioreactors
Cultivating quality cells is a key requirement for innovative approaches in regenerative medicine. Stirred-tank bioreactors are promising cultivation systems for the cultivation and differentiation of human induced pluripotent stem cells (hiPSCs), as they have the capacity to produce high cell numbers, allowing scale-up, and cutting-edge opportunities for improving the control…
Three-dimensional in vitro model to reproduce COVID-19-induced endothelial dysfunction
In a recent study posted to the bioRxiv* preprint server, researchers developed a three-dimensional (3D) in vitro tissue model to assess the effects of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) on the vascular endothelial cells. Study: SARS-CoV-2 spike protein induces endothelial dysfunction in 3D engineered vascular networks. Image Credit:…
Modelling urea cycle disorders using iPSCs
Gebhardt, R. & Matz-Soja, M. Liver zonation: Novel aspects of its regulation and its impact on homeostasis. World J. Gastroenterol. 20, 8491–8504 (2014). PubMed PubMed Central Article Google Scholar Summar, M. L. et al. The incidence of urea cycle disorders. Mol. Genet Metab. 110, 179–180 (2013). CAS PubMed PubMed Central …
iPSCs derived from infertile men carrying complex genetic abnormalities can generate primordial germ-like cells
Patients and controls The patient 1 was 38 years old and consulted for infertility after he and his partner had been trying to conceive for 2 years. The patient was the first child of unrelated parents, and he had four brothers and five sisters whose fertility status could not be determined…
Derivation and Culture of Human Induced Pluripotent Stem Cells – hiPSCs (cancelled)
Cost *Course fee £525 This is a residential course and the fee includes all accommodation and meals. *The course fee is subsidised by Wellcome Genome Campus Advanced Courses and Scientific Conferences and applies to non-commercial applicants. Please contact us for the commercial fee. BursariesLimited bursaries are available (up to 50%…
Faculty Opinions: hiPSCs for predictive modelling of neurodegenerative diseases: dreaming the possible.
Rivetti di Val Cervo P et al. Nature Reviews. Neurology. 2021 06; 17(6):381-392 doi.org/10.1038/s41582-021-00465-0PMID: 33658662 Show Details AbstractAuthors Human induced pluripotent stem cells (hiPSCs) were first generated in 2007, but the full translational potential of this valuable tool has yet to be realized. The potential applications of hiPSCs are especially…
TF differentiation of hiPSCs at Wellcome Sanger Institute
Salary per annum: c.£33,900 Contract Length: 2 years Here at the world-famous and internationally respected Wellcome Sanger Institute, we have an exciting opportunity for a postdoctoral researcher to perform high throughput screening for transcription factor-driven differentiation in human pluripotent stem cells (hiPSCs) in order to create better in vitro differentiated cells that more faithfully…
Nanofountain Probe Electroporation system enables efficient engineering of stem cells — ScienceDaily
One of the ultimate goals of medical science is to develop personalized disease diagnostics and therapeutics. With a patient’s genetic information, doctors could tailor treatments to individuals, leading to safer and more effective care. Recent work from a team of Northwestern Engineering researchers has moved the field closer to realizing…
Human induced pluripotent stem cells improve visual acuity, vascular health — ScienceDaily
Researchers at Indiana University School of Medicine, in collaboration with the University of Alabama at Birmingham and five other institutions, are investigating novel regenerative medicine approaches to better manage vascular health complications from type 2 diabetes that could someday support blood vessel repair in the eye among diabetic patients with…
Connecting science to medicine: tendon-like t
image: (a) Comparison of bio-tendons generated from GFP-iPSC-MSCs and Mkx-iPSC-MSCs. (b) SEM images of the surface layer of GFP-bio-tendon and Mkx-bio-tendon. view more Credit: Department of Systems BioMedicine, TMDU Researchers from Tokyo Medical and Dental (TMDU) develop artificial tendons in vitro from human stem cells that could fix common tendon injuries…
SUMO and stem cells
Stem cells are like master cells — they can divide to create more stem cells and be triggered by signals within and outside of themselves to give rise to virtually any cell type in the body. In order to exist as precursors, in what is called the pluripotent state, stem…
Multi-organoid system to simulate human liver
image: Enlarged view of the co-culture system view more Credit: Advanced Science Type 2 diabetes (T2DM) is a systematic multi-organ metabolic disease, which is characterized by dynamic interplay among different organs. Pancreatic islet-liver axis is closely associated with normal glucose regulation and homeostasis maintenance. The dysfunction of the interplay between these…
Multi-organoid system to simulate human liver-islet axis in normal and type 2 diabetes
Schematic of hiPSCs derived multi-organoid-on-chip system to model human liver-pancreatic islet axis in vitro. Credit: Tao Tingting Type 2 diabetes (T2DM) is a systematic multi-organ metabolic disease, which is characterized by dynamic interplay among different organs. Pancreatic islet-liver axis is closely associated with normal glucose regulation and homeostasis maintenance. The…
River BioMedics Raises Seed Investment to Progress its Cardiovascular Disease Programs
River BioMedics Raises Seed Investment to Progress its Cardiovascular Disease Programs • Eur 1.8 million seed funding to progress its pipeline of cardiovascular disease targets • Company appoints Nicky Cooper as its Chief Executive Officer • Financing round co-led by FIRST Fund, KIKK Capital and Oost NL Enschede, The Netherlands,…
Functioning induced liver cells from skin tissue
Figure 1. Urea metabolism is impaired in patient-derived human-induced pluripotent stem cells (hiPSCs) differentiated into hepatocytes (hiPSC-Heps). (A) Scheme showing the urea cycle detoxifying ammonia (NH4+) into nontoxic urea in five consecutive urea cycle enzyme (UCE)–mediated reactions in mitochondria in hepatocytes. (B) Urea secretion in four control hiPSC-Hep lines (Ctrl_1-4)…
Generation of Primordial Germ Cell-like Cells from iPSCs Derived from Turner Syndrome Patients
Figure 6 Dynamics of pluripotent and germ cells markers during hPGCLCs -TS induction. ( A… Figure 6 Dynamics of pluripotent and germ cells markers during hPGCLCs -TS induction. (A) Schematic protocol used to generate hPGCLCs. (B) hiPSCs-TS (p20), hEpiLC-TS (D.2), and hPGCLCs-TS (D.6) quantification of the relative expression of OCT4,…
An Optimized Culture Medium for Induced Pluripotent Stem Cells
In 2006, Kazutoshi Takahashi and Shinya Yamanaka made a revolutionary breakthrough when they reprogrammed terminally differentiated, lineage-restricted adult somatic cells into a pluripotent state via the expression of transcription factors Oct4, Sox2, Klf4, and c-Myc.1 Now, researchers can differentiate reprogrammed induced pluripotent stem cells (iPSCs) into any cell type and…
Hyperexcitability and Pharmacological Responsiveness of Cortical Neurons Derived from Human iPSCs Carrying Epilepsy-Associated Sodium Channel Nav1.2-L1342P Genetic Variant
Abstract With the wide adoption of genomic sequencing in children having seizures, an increasing number of SCN2A genetic variants have been revealed as genetic causes of epilepsy. Voltage-gated sodium channel Nav1.2, encoded by gene SCN2A, is predominantly expressed in the pyramidal excitatory neurons and supports action potential (AP) firing. One…
Scientific webinars on SelectScience
Genome engineering has revolutionized medical research through the ability to precisely edit the DNA sequence of cells. In combination with human induced pluripotent stem cells (hiPSCs), editing the genome can provide unprecedented insight into disease-causing mechanisms on a patient and tissue-specific basis. A major challenge, however, is the efficient generation…
