Tag: HPLC

. 2023 May 9;14(22):5945-5955. doi: 10.1039/d3sc00629h. eCollection 2023 Jun 7. Affiliations Expand Affiliations 1 Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing 30 Xueyuan Road, Haidian District Beijing 100083 P. R. China. 2 Department of Chemistry, Tsinghua University…

Cell lines and culture A549-hACE2 [a gift from Dr. Ben tenOever3], 293 T, HEK293, A549, and 3T3-L1 pre-adipocyte cell lines (refer to Supplementary Table 1) were cultured in DMEM and 10% FBS. U937, Calu-3, and HCT116 cells were cultured in RPMI, EMEM, and McCoy’s 5 A media, respectively, with 10% FBS. Caco-2 cells…

Ethics The Australian Genomics Acute Care study has Human Research Ethics Committee approval (HREC/16/MH/251). Parents provided informed consent for participation in the study, following genetic counseling. Study design and participants The Acute Care Genomics program is a national multi-site study delivering ultra-rapid genomic testing to critically ill pediatric patients with…

Adamek, M., Spohn, M., Stegmann, E. & Ziemert, N. Mining bacterial genomes for secondary metabolite gene clusters. Methods Mol. Biol. 1520, 23–47 (2017). CAS  PubMed  Google Scholar  Belknap, K. C., Park, C. J., Barth, B. M. & Andam, C. P. Genome mining of biosynthetic and chemotherapeutic gene clusters in Streptomyces…

Product Specific Information Description: The human soluble IL-2 receptor alpha chain (sIL-2Ra) is generated by proteolytic cleavage of the cell-surface bound receptor on activated T cells. The IL-2Ra (also known as CD25) is expressed by early progenitors of the T and B lineage, as well as by activated mature T…

This protocol describes the fabrication and characterization of a photoresponsive prodrug-dye nanoassembly. The methodology for drug release from the nanoparticles by light-triggered disassembly, including the light irradiation setup, is explicitly described. The drugs released from the nanoparticles following light irradiation exhibited excellent anti-proliferation effects on human colorectal tumor cells. This…

Tilman, D., Balzer, C., Hill, J. & Befort, B. L. Global food demand and the sustainable intensification of agriculture. Proc. Natl Acad. Sci. USA 108, 20260–20264 (2011). Article  ADS  CAS  PubMed  PubMed Central  Google Scholar  Hunter, M. C., Smith, R. G., Schipanski, M. E., Atwood, L. W. & Mortensen, D….

By Niomi Peckham, director, biologics pipeline development, Science-Global Biologics, United States Pharmacopeia The therapeutic potential of monoclonal antibodies (mAbs) came to the fore in the early 1970s when it was first demonstrated that pure protein could be obtained by fusing myeloma cells with the spleen cells of a mouse immunized…

Online Inquiry For Research Use Only. Do NOT use in humans or animals. For Research Use Only. Do NOT use in humans or animals. The product SIRGT24320WQ-LNA is a type of small interfering RNA (siRNA) that targets SNCA gene and regulates the expression of gene. The siRNA interferes with the…

Small interfering RNAs (siRNAs) usually occur as oligonucleotide duplexes. They can be analysed under nondenaturing conditions so that duplex formation is assured and excessive single-strands are detected. Alternatively, analysis of siRNAs can be conducted under denaturing conditions so that both sense and antisense strands are monitored. Analysis of siRNA under…

Job Description: The School of Pharmacy at Queen’s University Belfast, is currently seeking to appoint an exceptional candidate to the post of Research Fellow. The postholder will be an active member of the Biofunctional Nanomaterials group, led by Dr Garry Laverty, supporting the development of an enzyme-responsive peptide-mimetic hydrogel for…

We are seeking a highly motivated Sr. Associate Scientist within the Formulation Development Group (FDG). You will be involved with clinical and commercial formulation and drug product development activities including protein stability and characterization. We are designing and completing formulation and stability studies to support preclinical research and various phase-appropriate…

Waters has introduces its newest MaxPeak Premier oligonucleotide columns – now with ethylene bridged hybrid (BEH) particle technology in 300Å wide pore versions – providing excellent chromatographic resolution for long-mer oligonucleotide separations to support applications for nucleic acid therapeutics and cell and gene therapies. The new columns have all the benefits customers…

Thalassemia is a group of inherited blood disorders that affect the production of haemoglobin, a protein in red blood cells that carries oxygen throughout the body. People with thalassemia have an abnormal or reduced production of haemoglobin, which can cause anaemia and other health complications. What is Thalassaemia, what are…

Thalassemia is a genetic disease due to mutation in the genes encoding the globin chain of hemoglobin protein which forms the red pigment of blood. This leads to anemia due to ineffective ërythropoiesis” or formation of blood in bone marrow. This leads to enlargement of liver and spleen and bone…

Deoxynivalenol (DON) is one of the subgroup B mycotoxins, mainly produced by Fusarium species, and reported to be the predominant contaminant in the environment and food [17], [26]. The toxic effects of DON include immune modulation, disruption of intestinal barrier function, and cytotoxicity leading to cell death [13], [8]. Due…

Introduction Hyperuricemia is a disease caused by irregular purine metabolism, which is always accompanied by excessive production or abnormal excretion of uric acid (UA).1 Most notably, the overall incidence of hyperuricemia in China has been increasing over the past decade with the westernization of eating habits.2,3 However, there are blind…

Early on, one of the central challenges in mAb production was low product titer – but this has since been overcome by advances in cell line development and substantial improvements in cell-specific productivity, and further driven by broader progress in industrial bioproduction technology. Bioprocess optimization, generally achieved via optimization of…

Laboratory methods Tissue samples of lizards were collected over the course of several field seasons in Indonesia under appropriate permits as part of a biotic survey of mountains across the island of Sulawesi. Liver tissues were dissected and stored in RNA-later, which were incubated at ambient temperature for 24 to…

Preparation of GPMV GPMVs were prepared using different cell lines. Cells were cultured as follows: A549 (ATCC, Manassas, VA), HEK293 EBNA (herein referred to as HEK293, ATCC), HepG2 (ATCC), and Huh7 (ATCC) cells were kept in Dulbecco’s Modified Eagle’s Medium with high glucose (Sigma-Aldrich, Buchs, Switzerland) supplemented with 10% fetal…

Saudi Arabia Laboratories Market to reach USD 1.46 Billion by 2030 : Growth Driven by Rising Demand for High Quality Healthcare Services and Technological Advancements. Ohio, Cleveland, April 25, 2023 (GLOBE NEWSWIRE) — Market Overview: In Saudi Arabia, laboratories are vital for preserving the public’s health, fostering creativity, and advancing…

Introduction The metabolome is an incredibly diverse collection of small molecules (<1,500 Da) in biological systems involved in virtually every cellular process, including cellular energy production, macromolecule synthesis, epigenetic modifications, cell signalling and more (for recent reviews see [Citation1–6]). It responds rapidly (in seconds) to both internal (signalling, allostery) and external…

Pickar-Oliver A, Gersbach CA. The next generation of CRISPR–Cas technologies and applications. Nat Rev Mol Cell Bio. 2019;20:490–507. doi.org/10.1038/s41580-019-0131-5. Article  CAS  Google Scholar  Hendel A, Bak RO, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB, Bacchetta R, Tsalenko A, Dellinger D, Bruhn…

Abbas F, Eladl MA, El-Sherbiny M, Abozied N, Nabil A, Mahmoud SM et al (2022) Celastrol and thymoquinone alleviate aluminum chloride-induced neurotoxicity: behavioral psychomotor performance, neurotransmitter level, oxidative-inflammatory markers, and BDNF expression in rat brain. Biomed Pharmacother 151:113072 Article  CAS  Google Scholar  Abozaid OAR, Sallam MW, El-Sonbaty S, Aziza S,…

Swedish researchers have developed a microfluidic chip that they hope will allow for easier monitoring of monoclonal antibody (mAb) production. The protein cartridge, developed by a team from KTH Royal Institute of Technology in Stockholm, allows for the simultaneous analysis of up to four different proteins in the cell culture…

Optimizing prime editing systems for HSPCs We previously reported the use of prime editing to correct HBBS by plasmid transfection in HEK293T cells containing the SCD mutation, reaching up to 58% efficiency (Fig. 1a)24. In contrast to HEK293T cells, HSPCs are difficult to transfect with plasmid DNA but are amenable…

IgG plasmids Antibody sequences and Fc-tagged ACE2 were cloned into the CMV/R plasmid backbone for expression under a CMV promoter. The antibodies with variable HC/LC were cloned between the CMV promoter and the bGH poly(A) signal sequence of the CMV/R plasmid to facilitate improved protein expression. The variable region was…

To maximize reproducibility, a list of the reagents and resources used in this study, as well as their source and identifier, is provided in Supplementary Table 5. Experimental model and subject details Oral swab samples were obtained from bottlenose dolphins (Tursiops truncatus) managed by the U.S. Navy MMP Biosciences Division, Space…

Introduction Exposure of humans to aluminum has significantly increased worldwide. Potential sources of aluminum exposure include drinking water, food, medicines, vaccines, and aluminum cookware utensils. Aluminum bioaccumulation in the brain has been associated with neuroinflammatory and neurodegenerative changes that might predispose to Alzheimer’s disease (AD).1 Subchronic administration of Al3+ to rats…

More than one proteins in one row in mass spectrometry data 1 I have a matrix for whole cell mass spectrometry data. I found that many rows have more than one protein. I am new to proteomics. May I ask the reason behind this? proteomics mass_spectrometry • 63 views It…

To meet the challenge of sample depletion during preparation, there have been a number of recent advances in what is now a rapidly moving field of study of SCP, with important contributions from several investigators. Earlier work combined capillary electrophoresis (CE) with high-resolution mass spectrometry (HRMS) to analyze proteomes of…

To KK-W, Sridhar S, Chiu KH-Y, Hung DL-L, Li X, Hung IF-N, Tam AR, Chung TW-H, Chan JF-W, Zhang AJ-X, et al. Lessons learned 1 year after SARS-CoV-2 emergence leading to COVID-19 pandemic. Emerg Microbes Infect. 2021;10(1):507–35. Article  CAS  PubMed  PubMed Central  Google Scholar  Gao Q, Bao L, Mao H,…

PRESS RELEASE Published April 4, 2023 Creative BioMart GMP proteins, a sub-brand of Creative BioMart that provides comprehensive GMP proteins and peptides with defined identity, quality and purity, recently released new recombinant GMP proteins for in vitro diagnostics, therapeutic drug development, cosmetics, and the food industry. GMP grade protein is…

Plasmid construction The PVCpnf structural and accessory region (pvc1-16) and payload and regulatory region (Pdp1, Pnf and regulatory genes PAU_RS16570-RS24015) were synthesized de novo (GenScript) and cloned into pAWP78 and pBR322 backbones, respectively. All manipulations involving payload and regulatory plasmids (pPayload) involved standard PCR amplification with Phusion Flash 2x Master…

The oligo duplex synthesis service allows to obtain a fragment of dsDNA up to 220 bases long, not cloned, carefully controlled and in such quantity as to be used for a large number of applications. The service includes: synthesis, purification and QC (HPLC and Mass Check) of two complementary DNA…

Avgerinos GC, Drapeau D, Socolow JS, Mao J, Hsiao K and Broeze RJ (1990) Spin filter perfusion system for high density cell culture: production of recombinant urinary type plasminogen activator in CHO cells. Bio/Technology 8: 54–58. Article  CAS  Google Scholar  Bichay JT, Adams EG, Inch RW, Brewer JE and Bhuyan…

Plasmid construction The DNA sequence of the IgA variable region was obtained from a hybridoma cell line infused with immune cells from dengue-infected volunteers in Thailand was established by SPYMEG technology in a previous work (Setthapramote et al. 2012). The following primers were used to clone the variable region of…

In this protocol, we describe how to prepare mammalian cells for single-cell proteomics analysis via mass spectrometry using commercially available reagents and equipment, with options for both manual and automatic pipetting. SCoPE2 can quantify thousands of proteins from thousands of single mammalian cells without antibodies. This protocol can help uncover…

Vaccines 2023, 11(3), 678; doi.org/10.3390/vaccines11030678 (registering DOI) Vaccines 2023, 11(3), 678; doi.org/10.3390/vaccines11030678 (registering DOI) Received: 6 February 2023 / Revised: 7 March 2023 / Accepted: 10 March 2023 / Published: 16 March 2023 Round 1 Reviewer 1 Report (Previous Reviewer 1) To Authors: The manuscript “Immunogenicity of COVID-eVax delivered by electroporation is…

SygRNA Cas9 Synthetic Modified tracrRNA; HPLC-purified SygRNA modified tracrRNA oligo is optimized for use with a target-specific crRNA or modified crRNA …The SygRNA™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) …16 nov. 2016 — SygRNA™-Synthetic Two Part CRISPR RNA…

Oligonucleotide Synthesis Market Oligonucleotides, also known as oligomers, are small DNA or RNA molecules that have a variety of uses in genetic testing, research, and forensics. PORTLAND, OREGON, UNITED STATES, March 6, 2023 /EINPresswire.com/ — Oligonucleotide synthesis is the process of chemically synthesizing short strands of DNA or RNA molecules….

Here, Mark Dickman, Professor in Bioanalytical Science and Engineering at the University of Sheffield, explores how analytical techniques such as liquid chromatography-mass spectrometry can support the manufacturing of mRNA therapeutics. Large RNA is rapidly emerging as a new class of therapeutics. Nowhere is this more publicly evident than in the development, regulatory…

Recombinant peptide production Recombinant expression of Xt3a, Xt3a-D1, Xt3a-D2 and IpTxA was performed using an E. coli expression system. A gene encoding the peptide was subcloned into an expression vector containing a coding region with poly-histidine purification tag as well as a solubility tag (MBP for Xt3a and SUMO for…

1 Introduction Recombinant therapeutic proteins, also known as biologics, have been successfully used to treat a wide range of diseases, including cancers, inflammatory disorders, and cardiovascular diseases (Walsh, 2018). Biosimilars with comparable efficacy and safety profiles have emerged as alternative options to innovator therapies. A biosimilar is a biologic that…

Introduction According to the global cancer statistics 2018, breast cancer has the highest global incidence (28.9%) and mortality rates (23.0%) in women.1 Chemotherapy, as a mainstay in clinical breast cancer treatment, is limited by severe side-effects resulting from the nonspecific drug distribution in the human body.2–4 What is worse, a…

Growing studies focusing on nuclear acid detection via the emerging CRISPR technique demonstrate its promising application. However, limited works solve the identification of non-nucleic acid targets, especially multiple small molecules. To address challenges for point-of-care testing (POCT) in complex matrices for healthcare, environment, and food safety, we developed CRISPR Cas12a-powered…

Overview siRNA synthesis has been globally recognized as one of the most effective ways for the silencing of target genes in cells or body, and is widely accepted due to its low cost and high transfection efficiency. The well-known siRNA synthesis is not only the optimum method for RNAi‐based clinical…

Animals As female mice/rats resist to HFD-induced obesity and NAFLD, male mice/rats were used to induce obesity and NAFLD model by HFD50. Male Wistar (~180 g body weight; 8 weeks old) rats were purchased from Shandong Laboratory Animal Center with the permission number of SCXK 2014–0007 and raised under thermoneutral housing…

Teilen:  12.12.2022 13:08 Measuring plant resistance The scientists at the Leibniz Institute of Plant Biochemistry (IPB) are set to launch a new interdisciplinary research program in January 2023, which will consolidate existing capacities in high-performance analytical instruments with expertise in the fields of bioorganic chemistry, metabolomics, and chemo- and bioinformatics….

New York, United States—Nov 22, 2022—BOC Sciences leverages its profound experience in RNA therapeutics and innovative delivery platforms to offer high-quality and cost-effective siRNA molecules with various fluorescence labels, chemical modifications, and custom siRNAs to meet customers’ needs.   siRNA is a class of small, double-stranded, non-coding RNA molecules. siRNA regulates eukaryotic…

Cells and viruses hACE2 expressing BHK-21 (BHK-21-hACE2) and Vero-E6 cells were provided by State Key Laboratory of Virology, Wuhan University. Vero-E6 cells were cultured in DMEM (Gibco, Cat.11995–040) supplemented with 10% fetal bovine serum (Gibco, Cat.10091–148) and 1% penicillin and streptomycin solution (BasalMedia, Cat.S110JV) at 37°C with 5% CO2. BHK-21-hACE2…

Ilyas, M. et al. Environmental and health impacts of industrial wastewater effluents in Pakistan: A review. Rev. Environ. Health 34, 171–186 (2019). MathSciNet  PubMed  CAS  Google Scholar  Roy, M. A. et al. A Metagenomic approach to evaluating surface water quality in Haiti. Int. J. Environ. Res. Public Health 15, 2211…

Cell culture Human glioblastoma cells (U87MG and U87MG-Luc) and human embryonic kidney cells (HEK293) were obtained from the American Type Culture Collection (Manassas, Va.). Cells were cultured in Dulbecco’s Modified Eagle Medium supplemented with 10% fetal bovine serum (Gibco™ Fetal Bovine Serum South America, Thermo Scientific Fisher-US), 2 mM l-glutamine, 50 U/ml…

Chemical modification of biomolecules is an impressive way of nature to expand their functional diversity. Like DNA and proteins, RNA molecules also undergo a variety of chemical modifications that allow them to enrich their topological potential and perform a variety of functions that go beyond their capacity to encode proteins…

Phenolic acids are important phytochemicals that are present in whole grains. They possess bioactive properties such as antioxidant protective functions. This work aimed at reporting on a generalized method for the HPLC identification, total phenolic content estimation, and determination of the antioxidant capacity of phenolic acids in cereals and legumes….

Structural formula of 7-Deaza-7-propargylamino-ddGTP For research use only! Envio: shipped on blue ice Condições de armazenamento: store at -20 °CShort term exposure (up to 1 week cumulative) to ambient temperature possible. Validade: 12 months after date of delivery Fórmula molecular: C14H20N5O12P3 (free acid) Peso molecular: 543.26 g/mol (free acid) Pureza:…

The goal of this protocol is to detect phenolic metabolites in plasma using a semi-targeted chromatography-mass spectrometry method. The goal of this protocol is to detect phenolic metabolites in plasma by a semi-targeted UPLC-MS/MS method. Plasma proteins were precipitated with ethanol and samples concentrated, and re-suspended in the mobile phase,…

Introduction Antibody-drug conjugates (ADCs) have been studied for decades as a promising cancer treatment. As compared to conventional chemotherapy, ADCs have a larger therapeutic window because of their ability to target antigen-expressing tumor cells.1 As ADC drug development technology continues to evolve, several ADC drugs are being introduced to the…

Please login to add product to your favorites. Part Number SureGuide sgRNA, variable yield, HPLC Add to Favorites + Create New list Item successfully added to your list Read more here: Source link

The aim of this study was to investigate the effects of two antihistaminic drugs, terfenadine and diphenhydramine on CYP2D6 activity by using debrisoquine as a model substrate. The study was carried out as an in vivo single-dose study in 12 young, healthy men. All volunteers had previously been identified as…

We wish you a good luck and have a prosperous career. Working at Labcorp | Jobs and Careers at Labcorp 15 GNeuS Postdoc Positions in Neutron Science of 24 Months Each (Full-time Job) FZJ – Forschungszentrum Jülich. What other similar jobs are there to Laboratory jobs in Germany? Clinical Laboratory…

Plain Language Summary Fluoropyrimidine (FP; 5-FU, capecitabine) is a common chemotherapy used to treat many different cancers, including cancer of the colon and rectum, upper digestive tract, breast and head and neck. Cancer patients who receive FP chemotherapy are at risk of developing severe side effects from their treatment. A…

Introduction Prostate cancer (PCa) is one of the most common genitourinary system malignant tumor in men worldwide. In Asian countries, many patients with PCa are often diagnosed at an advanced stage maybe mostly because of a large population base with relatively backward economic development and imperfect cancer screening system of…

Introduction Diabetes is a very complex disease characterized by the presence of chronic hyperglycemia. Clinically, insulin-dependent type 1 and non-insulin-dependent type 2 are the main types of diabetes. Among them, type 2 diabetes mellitus (T2DM, [MIM125853]) is a common endocrine disorder affecting approximately 10% of adult population.1 In most cases,…

A healthy soil nourishes plants and animals, purifies water and air, and promotes sustainable agriculture. Characteristic for highly complex and competitive soil ecosystems are the frequent and direct interactions between all soil-dwelling microorganisms, animals, and plants (1, 2), all of which need to be provided with minerals and carbon sources….

Significance Class A penicillin-binding proteins (aPBPs) assemble the bacterial cell wall and are the targets of penicillin and related β-lactam antibiotics. In gram-negative bacteria, the aPBPs require outer membrane lipoproteins to function. However, little is known about how these proteins promote the activity of their cognate synthases in cells. Here,…