Tag: HPLC

Multiple Job openings at Gujarat Biotechnology Research Centre

Government of Gujarat has established Gujarat State Biotechnology Mission (GSBTM) as a nodal agency to develop overall development of biotechnology in the state in 2004. Major activities of GSBTM is promoting research and development in the field of biotechnology and supporting the development of biotechnology industries in the state. Besides…

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Bioactive glycans in a microbiome-directed food for children with malnutrition

Collection and handling of biospecimens obtained from participants in the randomized controlled clinical study of the efficacy of MDCF-2 The human study entitled ‘Community-based clinical trial with microbiota-directed complementary foods (MDCFs) made of locally available food ingredients for the management of children with primary moderate acute malnutrition (MAM)’ was approved…

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Reducing variability in protein purification

In this article, Analytik Jena details what technology can make protein purification less variable and faster with the help of liquid-handling equipment. The CyBio FeliX and PhyTips® can conduct 96 targeted protein purifications in 120 minutes, requiring no hands-on time. These products are intended for drug discovery professionals in the…

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Release Testing Of AAV Gene Therapies For Clinical Trials

By Mark Haydock, biologics CMC consultant, Agile Biologics Consulting LLC Adeno-associated virus (AAV) gene therapies that are designed to deliver a therapeutic transgene to patients are complex products that can be challenging to manufacture. Because of their complexity, a variety of analytical methods are required to ensure that these viral…

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Navigating the Frontier of Circular RNA Therapeutics: Insights from the Experts

Join our panel of experts as we explore the exciting field of circular RNA therapeutics. The landscape of therapeutic innovation is continually evolving, and one of the latest frontiers captivating the attention of analytical scientists is Circular RNA (circRNA). As we gear up for our expert discussion forum on exploring…

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Phenotypic drug-susceptibility profiles and genetic analysis based on whole-genome sequencing of Mycobacterium avium complex isolates in Thailand

Abstract Mycobacterium avium complex (MAC) infections are a significant clinical challenge. Determining drug-susceptibility profiles and the genetic basis of drug resistance is crucial for guiding effective treatment strategies. This study aimed to determine the drug-susceptibility profiles of MAC clinical isolates and to investigate the genetic basis conferring drug resistance using…

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Impact of N221S missense mutation in human ribonucleotide reductase small subunit b on mitochondrial DNA depletion syndrome

hRRM2B mutagenesis study The fatal consequences of N221S mutation7 led to a thorough investigation of the impacts of N221 side chain, a conserved residue in RNR small subunit. Site-directed mutagenesis studies involving six N221 constructs were performed to elucidate the molecular mechanism of N221S related MDDS. The mutations were designed…

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siRNA, Anti-Human LARP4B, 2′-OMe (SIRGT36755WQ-2OMe)

Online Inquiry For Research Use Only. Do NOT use in humans or animals. For Research Use Only. Do NOT use in humans or animals. The product SIRGT36755WQ-2OMe is a type of small interfering RNA (siRNA) that targets LARP4B gene and regulates the expression of gene. The siRNA interferes with the…

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siRNA, Anti-Human WIPF1, LNA (SIRGT24895WQ-LNA)

Online Inquiry For Research Use Only. Do NOT use in humans or animals. For Research Use Only. Do NOT use in humans or animals. The product SIRGT24895WQ-LNA is a type of small interfering RNA (siRNA) that targets WIPF1 gene and regulates the expression of gene. The siRNA interferes with the…

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siRNA, Anti-Human ADCY5, 2′-MOE (SIRGT03069WQ-2MOE)

Online Inquiry For Research Use Only. Do NOT use in humans or animals. For Research Use Only. Do NOT use in humans or animals. The product SIRGT03069WQ-2MOE is a type of small interfering RNA (siRNA) that targets ADCY5 gene and regulates the expression of gene. The siRNA interferes with the…

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siRNA, Anti-Human CAD (SIRGT00532WQ) – Creative Biolabs

Online Inquiry For Research Use Only. Do NOT use in humans or animals. For Research Use Only. Do NOT use in humans or animals. The product SIRGT00532WQ is a type of small interfering RNA (siRNA) that targets CAD gene and regulates the expression of gene. The siRNA interferes with the…

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The DNA-binding induced (de)AMPylation activity of a Coxiella burnetii Fic enzyme targets Histone H3

Statistics and reproducibility Anti-AMP IP for LC-MS/MS analysis was performed in three independent biological replicates (n = 3). TSA assay data represents technical triplicates. Time-resolved (de)AMPylation analyzed by LC-MS was performed as biological triplicates. CD measurements were performed in technical triplicates. Anisotropy data is shown as technical triplicates. Anisotropy measurements were repeated…

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Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2

List of reagents Chloramine-T trihydrate (Merck, Darmstadt, Germany), Copper(II)Sulphate, Penicillin, Streptomycin, RPMI-1640 (Sigma-Aldrich, Burlington, MA, USA), RPMI-1640 (Euroclone, Pero, Italy), DMEM low glucose (Sigma-Aldrich), Trypsin–EDTA (Sigma-Aldrich), Blasticidin hydrochloride (Sigma-Aldrich), Trichloroacetic acid (Merck), Fetal Bovine Serum (Merck, Darmstadt, Germany), Iodine 125 Radionuclide (Perkin Elmer, Waltham, Mass., USA), Iodogen™ iodination reagent (Pierce,…

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Correction of a homoplasmic mitochondrial tRNA mutation in patient-derived iPSCs via a mitochondrial base editor

Human induced pluripotent stem cells (iPSCs) Reprogramming and Culture This study was ethically approved by the Medical Ethics Committee of Nanjing Maternal and Child Health Care Hospital (2021KY-131), and informed consents were obtained from the patient’s legal guardian as well as the healthy donors, in accordance with the Declaration of…

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Evaluation of the Effect of CYP2D6 *3, *4,*10, and *17 Polymorphisms on the Pharmacokinetic of Tamoxifen and its Metabolites in Patients with Hormone-Positive Breast Cancer,Journal of Pharmaceutical and Biomedical Analysis

Background and Objective The high rate of interindividual variability in response to tamoxifen (TAM) in breast cancer patients with CYP2D6 polymorphism has been reported which affects the patient’s therapeutic outcome. The objective of this study was to investigate the pharmacogenomics of CYP2D6 genotyping in Iranian patients with breast cancer treated…

Continue Reading Evaluation of the Effect of CYP2D6 *3, *4,*10, and *17 Polymorphisms on the Pharmacokinetic of Tamoxifen and its Metabolites in Patients with Hormone-Positive Breast Cancer,Journal of Pharmaceutical and Biomedical Analysis

siRNA, Anti-Human ALKBH4, 2′-MOE (SIRGT43278WQ-2MOE)

Online Inquiry For Research Use Only. Do NOT use in humans or animals. For Research Use Only. Do NOT use in humans or animals. The product SIRGT43278WQ-2MOE is a type of small interfering RNA (siRNA) that targets ALKBH4 gene and regulates the expression of gene. The siRNA interferes with the…

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Structure and electromechanical coupling of a voltage-gated Na+/H+ exchanger

Expression and purification of SLC9C1 The gene encoding the S. purpuratus SLC9C1 protein (NP_001091927.1), referred to as SLC9C1, was synthesized (Thermo Fisher Scientific) and subcloned into the pcDNA3.1(+) vector followed by a TEV (Tobacco Etch Virus nuclear-inclusion-a endopeptidase) recognition site, eGFP and a TwinStrep tag at the C terminus. An…

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A Bioconductor workflow for processing, evaluating,…

Introduction Proteins are responsible for carrying out a multitude of biological tasks, implementing cellular functionality and determining phenotype. Mass spectrometry (MS)-based expression proteomics allows protein abundance to be quantified and compared between samples. In turn, differential protein abundance can be used to explore how biological systems respond to a perturbation….

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Antiviral type III CRISPR signalling via conjugation of ATP and SAM

Cloning Supplementary Table 1 shows the synthetic gene, DNA and RNA oligonucleotide sequences used in this study. The synthetic genes encoding B.fragilis Cas6, CorA, NrN and C. botulinum SAM lyase purchased as g-blocks (IDT) were codon-optimized for expression in E. coli C43 (DE3) via the vector pEhisV5TEV, which encodes eight…

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Phytochemical Characterization of Bio-active Compounds in Hydroethanolic Extract of Elaeocarpus ganitrus leaves using HPLC, LC-MS, and HPTLC Analyses

Abstract Bioactive compounds have various applications in different industries, including food, pharmaceutical, and cosmetic industries, demonstrating the need to identify the best-standardized technique to screen the phytochemical profile of medicinal plants. This study aimed to characterize the bioactive compounds in the hydroethanolic extracts of Elaeocarpus ganitrus leaves using various analytical…

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Rapid discovery of high-affinity antibodies via massively parallel sequencing, ribosome display and affinity screening

Construct design To transcribe and translate sequenced DNA clusters on an Illumina flow cell, our DNA constructs contained the following elements: a P5 adaptor, followed by a 28 nt unique barcode, a 27 nt unstructured spacer (5p UNS v2), a ribosome binding site, start codon, protein coding region, TolAk short linker, 2×…

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A thermostable type I-B CRISPR-Cas system for orthogonal and multiplexed genetic engineering

A thermostable type I-B CRISPR-Cas system from P. thermoglucosidasius Geobacillus and Parageobacillus are ubiquitous in a wide range of environments, growing at temperatures ranging from 35–80 °C29. To survey the distribution of thermostable type I CRISPR-Cas systems in these genera, we extracted all available genomes from CRISPRCasFinder database30, and identified Type…

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A novel dsDNA decamer-based electrochemical biosensor for selective determination of irinotecan active metabolite – SN38,Sensors and Actuators B: Chemical

This paper presents a novel biosensor for detecting the active metabolite of irinotecan, SN38, with high selectivity and sensitivity. The biosensor utilizes a dsDNA decamer (5’-GCGTTGTCGC-3’) that selectively interacts with SN38. The biosensor, consisting of a screen-printed graphite electrode with the dsDNA decamer as the recognition layer, demonstrates stability, sensitivity,…

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Recombinant Human EpCAM protein (ab151338)

Recombinant Human EpCAM protein is a HEK 293 Fragment protein 24 to 265 aa range, >95% purity, < 1 EU/µg endotoxin level and validated in SDS-PAGE, HPLC. Alternative names= EGP, ESA, KSA, gp4, AUA1, EGP2, Ly74, M1S2, M4S1, MK 1, 17 1A, CD326, CO17A, DIAR5, EGP 2, EGP40, EPCAM, KS1/4,…

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Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system | AMB Express

Establishment of PCR-lbCas12 DETECTR The whole detection process is illustrated in Fig. 1a. According to the data of infected persons from the Medical Laboratory of Shenzhen Luohu People’s Hospital, Salmonella is always among the top 5 positive Gram-negative (G−) bacilli in both 2021 and 2020 (Fig. 1b). To obtain efficient and specific…

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mRNA vaccine quality analysis using RNA sequencing

Design and synthesis of reference plasmid A reference construct was first designed, with the intention of optimising the production of RNA therapeutics for pre-clinical research. The coding sequence of eGFP30 was selected as a reporter in the coding region, as its protein product can be assayed simply through Flow cytometry…

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Technologies | Abnova

Wheat Germ Protein Expression Proximity Ligation Assay Mouse Monoclonal Antibody Production Rabbit Monoclonal Antibody (Phage Antibody Library) Chimera RNAi Fluorescence In Situ Hybridization (FISH) Membrane Protein Proteoliposomes Protein and Post-Translational Modification Quantification ACTN4 FISH Probe CytoQuest™ CR LiquidCell™ Cell-Surface Vimentin (CSV) Monoclonal Antibody mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization) Next-Generation Sequencing DNAx™ Immune SAMx™ Immune COVID-19 Humanized Monoclonal Antibody COVID-19 SAM Universal Vaccine COVID-19 circRNA Vaccine Omicron…

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Guidelines For mRNA Drug Product Manufacturing And Quality Control

By John F. Cipollo, United States Pharmacopeia Messenger RNA (mRNA) is the template for protein synthesis. It serves as an intermediate to transfer the genetic information from DNA in the nucleus to the ribosome sites for translation to protein. It can encode virtually any protein, making it extremely attractive as…

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The Chromosomal Virulence D | IDR

Introduction The clinical symptoms and signs of pulmonary disease caused by nontuberculous mycobacteria (NTM) are very similar to tuberculosis, so differentiating the two becomes extremely challenging. Both diseases manifest cough, expectoration, hemoptysis, chest pain, and other respiratory symptoms, as well as systemic symptoms, such as fatigue and anorexia.1–3 Furthermore, NTM…

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Recombinant Human FGFR2 protein(IIIb), His-Avi-tagged FGFR2-055H

Description : Four distinct genes encoding closely related FGF receptors, FGF R1 – 4, are known. All four genes for FGF Rs encode proteins with an N-terminal signal peptide, three immunoglobulin (Ig)-like domains, an acid-box region containing a run of acidic residues between the IgI and IgII…

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Symmetric inheritance of parental histones governs epigenome maintenance and embryonic stem cell identity

The research in this study was conducted under the ethical approval of the Danish Regulatory Authority under project license 2018-15-0201-01520. Cell culture and differentiation assays WT, MCM2-2A, MCM2-R and POLE4-KO mouse ESCs used in this study were derived from the male, E14JU cell line with a 129/Ola background81. Genome editing…

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Bioinformatics job with Seqirus | 1401899066

The Opportunity You will develop assays for release and characterization of mRNAs and LNPs. You will perform sample testing of UPLC/HPLC, CE, analytical characterization of drug substance and drug products, and support process development. You will contribute to analytical assay development, optimization, and in-process control to support product and process…

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An IgM-like inhalable ACE2 fusion protein broadly neutralizes SARS-CoV-2 variants

Protein expression hACE2-hIgG1 was constructed by fusing the extracellular domain of human ACE2 (amino acid 19–615) (UniProt Q9BYF1) to the human IgG1 hinge and Fc (UniProt P01857). H374N and H378N mutations were introduced to inactivate the Zinc metallo-enzyme activity17. HH-120 was constructed by fusing hACE2-hIgG1 with an IgM tailpiece (PTLYNVSLVMSDTAGTCY)33….

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The non-classical major histocompatibility complex II protein SLA-DM is crucial for African swine fever virus replication

Cell lines and viruses Cell lines were received from the cell culture collection for veterinary medicine (CCVM) of the Friedrich-Loeffler-Institut (FLI). The highly passaged wild boar lung cell line (WSL-R-HP, #1346; abbreviated as WSL) was maintained in Ham’s F12 cell culture medium (Ham’s F-12, 5.32 g/L; IMDM, 8.80 g/L; NaHCO3, 2.45 g/L; pH 7.2)…

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Oligonucleotides | Thermo Fisher Scientific

Use this tool to design a custom DNA oligonucleotide, with any of a variety of 3′ and 5′ modifications, at scales ranging from 25 nmole to 10 µmole. Select desired purification method (normal or reversed-phase chromatography, HPLC, or PAGE). Use this tool to design a custom DNA oligonucleotide, with any…

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Bioinformatics job with Seqirus | 1401887420

The Opportunity You will develop assays for release and characterization of mRNAs and LNPs. You will perform sample testing of UPLC/HPLC, CE, analytical characterization of drug substance and drug products, and support process development. You will contribute to analytical assay development, optimization, and in-process control to support product and process…

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CpG-SNP site methylation regulates allele-specific expression of MTHFD1 gene in type 2 diabetes

CpG-SNP site methylation regulates allele-specific expression of MTHFD1 gene in type 2 diabetes – Fingerprint — Manipal Academy of Higher Education, Manipal, India Sort by Weight Alphabetically Biochemistry, Genetics and Molecular Biology Nested Gene 100% Methylation 100% Single Nucleotide Polymorphism 100% Allele 100% DNA Methylation 33% Genetics 33% Carbon Metabolism…

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Rznomics Presents Its Own Circular RNA Platform Technology

SEONGNAM, South Korea, Aug. 3, 2023 /PRNewswire/ — Rznomics developed a novel platform technology termed ‘self-circularized RNA structure’ that can efficiently and simply generate circular RNA and overcome the limitations of existing technology. Rznomics published an article regarding its development strategy and efficacy in the journal ‘Molecular Therapy Nucleic Acids.’…

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Chemically programmed STING-activating nano-liposomal vesicles improve anticancer immunity

Rational design of STING-activating pro-drugs for liposomal formulation A recently discovered non-nucleotide STING agonist, MSA-2, was selected to test the validity of our drug design. To facilitate the liposomal formulation of MSA-2, four synthetic MSA-2 derivatives (compounds 1–4) were initially constructed via ester bonds using MP-typed alkanols of varying lengths…

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Streamlining DNA Mutation Detection: A Breakthrough with Phosphorothioated DNA-guided Endonuclease Excision

Challenges in DNA Mutation Detection: Molecular diagnostics of cancer has underscored the significance of driver genetic alterations in tumor growth and metastasis1, 2. Although genetic variations hold abundant diagnostic and therapeutic clues for cancer patients, detecting mutations in cfDNA proves to be a major challenge due to their typically low…

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Lab Assistant II – Molecular Engineering – Seattle

The Position Roche Diagnostics acquired Stratos Genomics ( SBX Nanopore Sequencing ) in 2020 for its unique chemistry that advances DNA sequencing by nanopore, Sequencing by Expansion (SBX™). You will engineer and synthesize the molecular building blocks for our novel technology. As such you will need to demonstrate the desire…

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Advances in DNA methylation and demethylation in medicinal plants: a review

Liu WJ, Zhang JH, Luo H (2018) Status and prospects of herbal epigenomics. Sci China Press 48:365–378. doi.org/10.1360/n052017-00191 Article  Google Scholar  Yuan C, Zhang SW, Niu Y, Tang QL, Wei DY, Wang ZM (2020) Advances in research on the mechanism of DNA methylation in plants. Chin J Biotechnol 36:838–848. doi.org/10.13345/j.cjb.190373…

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Development of siRNA-Based Therapies

In recent years, multiple clinical trials have demonstrated the tremendous potential of RNA interference (RNAi) drugs targeting disease-causing genes to treat diseases that have plagued humans for years. Harnessing the power of RNAi, our company is committed to providing customers with specially designed small interfering RNAs (siRNAs) to knock down the…

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Research Officer (Bioinformatics, HPLC or spectometry )

Research Officer (Bioinformatics, HPLC or spectometry ) By continuing to use and navigate this website, you are agreeing to the use of cookies. Accept Close National Neuroscience Institute of Singapore Posting Date:  20 Jul 2023 A research position in Neuroscience is available at the National Neuroscience Institute. We are looking…

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An RNA modification enzyme directly senses reactive oxygen species for translational regulation in Enterococcus faecalis

Bacteria strains, plasmids, and growth conditions Enterococcus faecalis strains OG1RF and V583 are grown in tryptic soy broth (TSB) or plated on tryptic soy agar under aerobic conditions at 37 °C. All mutant strains are derivatives of OG1RF. The rlmN knockout in OG1RF (ΔrlmN) was generated by an in-frame deletion of…

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How to select the right CDMO for innovative antibody development

Choosing the right Contract Development and Manufacturing Organization (CDMO) is a pivotal decision for biopharmaceutical companies embarking on innovative antibody development projects.  With the industry’s increasing demand for qualified multidisciplinary experts, biopharma companies are seeking trusted CDMO partners for specialized support and capacity. Additionally, various pressures complicate the process and…

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siRNA – Microsynth – CH

    siRNA (small interfering ribonucleic acid) is a type of RNA that is involved in a number of biological processes, most notably RNA interference. RNA interference is a regulatory process that is used to control and limit the expression of specific genes. Regulatory active siRNA consists of annealed, complementary…

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Deoxyribonucleic Acid Barcoding for the Identification of Botanicals

Hebert PD, Stoeckle MY, Zemlak TS, Francis CM (2004) Identification of birds through DNA barcodes. PLoS Biol 2:e312 CrossRef  PubMed  PubMed Central  Google Scholar  International Barcode of Life Conference (2011) www.dnabarcodes2011.org/ China Plant BOL Group, Li DZ, Gao LM, Li HT, Wang H, Ge XJ, Liu JQ, Chen ZD, Zhou…

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Differential interactions of selected phytocannabinoids with CYP2D6 polymorphisms

Phytocannabinoids (pCBs) refer to compounds from the cannabis plant (Cannabis sativa), also known as cannabinoids , we found that pCBs can be differentially metabolized by different cytochrome P450 (CYP) and different polymorphisms of human CYP2D6, In addition, inhibition or activation of enzymes involved in drug metabolism by pCB will in…

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Modelling ligand depletion for simultaneous affinity and binding site quantification on cells and tissue

Cell culture All cells were maintained under standard cell culture conditions (37 °C, 5% CO2, saturated humidity). CHO cells stably transfected with human GIPR (gastric inhibitory polypeptide receptor) were obtained from InSCREENeX GmbH (Germany) and cultured in Dulbecco`s Modified Eagle`s Medium/Nutrient Mixture F-12 Ham (Cat.No. D6421, Sigma) supplemented with 5% fetal bovine…

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How do tumor exosomes communicate cancer progression?

Exosomes are extracellular vesicles ranging between 30 and 150 nm in diameter that are released from different kinds of cells, including tumor cells. They have the potential to induce apoptosis, modulate the immune system, and work as biomarkers for diagnosis. As a key component of cell-to-cell communication, exosomes can control the…

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University of North Carolina at Charlotte hiring Postdoc – Bioinformatics & Genomics in Charlotte, North Carolina, United States

Position Number: POST40 Department: Coll of Computing&Informatics (Col) Employment Type: Temporary – Part-time Months Per Year: 12 Essential Duties And Responsibilities The Yohe Lab is seeking a postdoctoral researcher position to investigate the developmental origins of bat diversity. The project investigates craniofacial anatomy and the development of sensory systems in…

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Non disseminative nano-strategy against in vivo Staphylococcus aureus biofilms

Materials The lipid cetyl palmitate was a kind gift from Gattefossé (Gattefossé SAS, Saint-Priest, France). DSPE-PEG2000-NH2 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt)) was purchased from Avanti Polar Lipids Inc. (Alabaster, Alabama, USA). D-Phenylalanine, D-Proline, D-Tyrosine, triethylamine (TEA), dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), sodium hydroxide (NaOH), sodium chloride, moxifloxacin hydrochloride (MOX), farnesol 95%, Tween®80,…

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Multiplexed single-cell 3D spatial gene expression analysis in plant tissue using PHYTOMap

Sample preparation Arabidopsis thaliana accession Col-0 seeds (hereafter Arabidopsis) were sown on square plates containing Linsmaier and Skoog medium (Caisson Labs, catalogue no. LSP03) with 0.8% sucrose solidified with 1% agar (Caisson Labs, catalogue no. A038). Plates were kept vertically for 5 days in a growth chamber under an 8:16 h light/dark…

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Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA

. 2023 May 9;14(22):5945-5955. doi: 10.1039/d3sc00629h. eCollection 2023 Jun 7. Affiliations Expand Affiliations 1 Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing 30 Xueyuan Road, Haidian District Beijing 100083 P. R. China. 2 Department of Chemistry, Tsinghua University…

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A viral pan-end RNA element and host complex define a SARS-CoV-2 regulon

Cell lines and culture A549-hACE2 [a gift from Dr. Ben tenOever3], 293 T, HEK293, A549, and 3T3-L1 pre-adipocyte cell lines (refer to Supplementary Table 1) were cultured in DMEM and 10% FBS. U937, Calu-3, and HCT116 cells were cultured in RPMI, EMEM, and McCoy’s 5 A media, respectively, with 10% FBS. Caco-2 cells…

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Integrated multi-omics for rapid rare disease diagnosis on a national scale

Ethics The Australian Genomics Acute Care study has Human Research Ethics Committee approval (HREC/16/MH/251). Parents provided informed consent for participation in the study, following genetic counseling. Study design and participants The Acute Care Genomics program is a national multi-site study delivering ultra-rapid genomic testing to critically ill pediatric patients with…

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Comparative genome features and secondary metabolite biosynthetic potential of Kutzneria chonburiensis and other species of the genus Kutzneria

Adamek, M., Spohn, M., Stegmann, E. & Ziemert, N. Mining bacterial genomes for secondary metabolite gene clusters. Methods Mol. Biol. 1520, 23–47 (2017). CAS  PubMed  Google Scholar  Belknap, K. C., Park, C. J., Barth, B. M. & Andam, C. P. Genome mining of biosynthetic and chemotherapeutic gene clusters in Streptomyces…

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Human CD25 (IL-2R alpha) Recombinant Protein (34-8702-82)

Product Specific Information Description: The human soluble IL-2 receptor alpha chain (sIL-2Ra) is generated by proteolytic cleavage of the cell-surface bound receptor on activated T cells. The IL-2Ra (also known as CD25) is expressed by early progenitors of the T and B lineage, as well as by activated mature T…

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Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

This protocol describes the fabrication and characterization of a photoresponsive prodrug-dye nanoassembly. The methodology for drug release from the nanoparticles by light-triggered disassembly, including the light irradiation setup, is explicitly described. The drugs released from the nanoparticles following light irradiation exhibited excellent anti-proliferation effects on human colorectal tumor cells. This…

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Selective control of parasitic nematodes using bioactivated nematicides

Tilman, D., Balzer, C., Hill, J. & Befort, B. L. Global food demand and the sustainable intensification of agriculture. Proc. Natl Acad. Sci. USA 108, 20260–20264 (2011). Article  ADS  CAS  PubMed  PubMed Central  Google Scholar  Hunter, M. C., Smith, R. G., Schipanski, M. E., Atwood, L. W. & Mortensen, D….

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Heres How USP mAb Standards Support Fast-Evolving Platform Approaches

By Niomi Peckham, director, biologics pipeline development, Science-Global Biologics, United States Pharmacopeia The therapeutic potential of monoclonal antibodies (mAbs) came to the fore in the early 1970s when it was first demonstrated that pure protein could be obtained by fusing myeloma cells with the spleen cells of a mouse immunized…

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siRNA, Anti-Human SNCA, LNA (SIRGT24320WQ-LNA)

Online Inquiry For Research Use Only. Do NOT use in humans or animals. For Research Use Only. Do NOT use in humans or animals. The product SIRGT24320WQ-LNA is a type of small interfering RNA (siRNA) that targets SNCA gene and regulates the expression of gene. The siRNA interferes with the…

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How to choose the optimum IP-RP conditions for analysis of denatured or non-denatured siRNA duplexes Chromatography Today

Small interfering RNAs (siRNAs) usually occur as oligonucleotide duplexes. They can be analysed under nondenaturing conditions so that duplex formation is assured and excessive single-strands are detected. Alternatively, analysis of siRNAs can be conducted under denaturing conditions so that both sense and antisense strands are monitored. Analysis of siRNA under…

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Research Fellow, School of Pharmacy job with QUEENS UNIVERSITY BELFAST

Job Description: The School of Pharmacy at Queen’s University Belfast, is currently seeking to appoint an exceptional candidate to the post of Research Fellow. The postholder will be an active member of the Biofunctional Nanomaterials group, led by Dr Garry Laverty, supporting the development of an enzyme-responsive peptide-mimetic hydrogel for…

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Sr. Associate Scientist, Formulation Development (siRNA, AAV, gene therapy) in Tarrytown, NY

We are seeking a highly motivated Sr. Associate Scientist within the Formulation Development Group (FDG). You will be involved with clinical and commercial formulation and drug product development activities including protein stability and characterization. We are designing and completing formulation and stability studies to support preclinical research and various phase-appropriate…

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New column technology | Scientist Live

Waters has introduces its newest MaxPeak Premier oligonucleotide columns – now with ethylene bridged hybrid (BEH) particle technology in 300Å wide pore versions – providing excellent chromatographic resolution for long-mer oligonucleotide separations to support applications for nucleic acid therapeutics and cell and gene therapies. The new columns have all the benefits customers…

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Knowing thalassemia, its symptoms and treatments | Liufestyle Health

Thalassemia is a group of inherited blood disorders that affect the production of haemoglobin, a protein in red blood cells that carries oxygen throughout the body. People with thalassemia have an abnormal or reduced production of haemoglobin, which can cause anaemia and other health complications. What is Thalassaemia, what are…

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Understanding Thalassemia Genetic Mutations Haemoglobin Production Symptoms Treatment

Thalassemia is a genetic disease due to mutation in the genes encoding the globin chain of hemoglobin protein which forms the red pigment of blood. This leads to anemia due to ineffective ërythropoiesis” or formation of blood in bone marrow. This leads to enlargement of liver and spleen and bone…

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“Lollipop” particle counting immunoassay based on antigen-powered CRISPR-Cas12a dual signal amplification for the sensitive detection of deoxynivalenol in the environment and food samples

Deoxynivalenol (DON) is one of the subgroup B mycotoxins, mainly produced by Fusarium species, and reported to be the predominant contaminant in the environment and food [17], [26]. The toxic effects of DON include immune modulation, disruption of intestinal barrier function, and cytotoxicity leading to cell death [13], [8]. Due…

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Mechanism of radix astragali therapy against hyperuricemia

Introduction Hyperuricemia is a disease caused by irregular purine metabolism, which is always accompanied by excessive production or abnormal excretion of uric acid (UA).1 Most notably, the overall incidence of hyperuricemia in China has been increasing over the past decade with the westernization of eating habits.2,3 However, there are blind…

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Improving the Potency of Monoclonal Antibodies With Fucosylation

Early on, one of the central challenges in mAb production was low product titer – but this has since been overcome by advances in cell line development and substantial improvements in cell-specific productivity, and further driven by broader progress in industrial bioproduction technology. Bioprocess optimization, generally achieved via optimization of…

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Highly-multiplexed and efficient long-amplicon PacBio and Nanopore sequencing of hundreds of full mitochondrial genomes | BMC Genomics

Laboratory methods Tissue samples of lizards were collected over the course of several field seasons in Indonesia under appropriate permits as part of a biotic survey of mountains across the island of Sulawesi. Liver tissues were dissected and stored in RNA-later, which were incubated at ambient temperature for 24 to…

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High efficiency preparation of monodisperse plasma membrane derived extracellular vesicles for therapeutic applications

Preparation of GPMV GPMVs were prepared using different cell lines. Cells were cultured as follows: A549 (ATCC, Manassas, VA), HEK293 EBNA (herein referred to as HEK293, ATCC), HepG2 (ATCC), and Huh7 (ATCC) cells were kept in Dulbecco’s Modified Eagle’s Medium with high glucose (Sigma-Aldrich, Buchs, Switzerland) supplemented with 10% fetal…

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Growth Driven by Rising Demand for High Quality Healthcare Services and Technological Advancements.

Saudi Arabia Laboratories Market to reach USD 1.46 Billion by 2030 : Growth Driven by Rising Demand for High Quality Healthcare Services and Technological Advancements. Ohio, Cleveland, April 25, 2023 (GLOBE NEWSWIRE) — Market Overview: In Saudi Arabia, laboratories are vital for preserving the public’s health, fostering creativity, and advancing…

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Prior metabolite extraction fully preserves RNAseq quality and enables integrative multi-‘omics analysis of the liver metabolic response to viral infection

Introduction The metabolome is an incredibly diverse collection of small molecules (<1,500 Da) in biological systems involved in virtually every cellular process, including cellular energy production, macromolecule synthesis, epigenetic modifications, cell signalling and more (for recent reviews see [Citation1–6]). It responds rapidly (in seconds) to both internal (signalling, allostery) and external…

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Middle-out sequence confirmation of CRISPR/Cas9 single guide RNA (sgRNA) using DNA primers and ribonuclease T1 digestion

Pickar-Oliver A, Gersbach CA. The next generation of CRISPR–Cas technologies and applications. Nat Rev Mol Cell Bio. 2019;20:490–507. doi.org/10.1038/s41580-019-0131-5. Article  CAS  Google Scholar  Hendel A, Bak RO, Clark JT, Kennedy AB, Ryan DE, Roy S, Steinfeld I, Lunstad BD, Kaiser RJ, Wilkens AB, Bacchetta R, Tsalenko A, Dellinger D, Bruhn…

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Neuro-amelioration of Ficus lyrata (fiddle-leaf fig) extract conjugated with selenium nanoparticles against aluminium toxicity in rat brain: relevance to neurotransmitters, oxidative, inflammatory, and apoptotic events

Abbas F, Eladl MA, El-Sherbiny M, Abozied N, Nabil A, Mahmoud SM et al (2022) Celastrol and thymoquinone alleviate aluminum chloride-induced neurotoxicity: behavioral psychomotor performance, neurotransmitter level, oxidative-inflammatory markers, and BDNF expression in rat brain. Biomed Pharmacother 151:113072 Article  CAS  Google Scholar  Abozaid OAR, Sallam MW, El-Sonbaty S, Aziza S,…

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Revolutionizing Monoclonal Antibody Production Monitoring

Swedish researchers have developed a microfluidic chip that they hope will allow for easier monitoring of monoclonal antibody (mAb) production. The protein cartridge, developed by a team from KTH Royal Institute of Technology in Stockholm, allows for the simultaneous analysis of up to four different proteins in the cell culture…

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Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice

Optimizing prime editing systems for HSPCs We previously reported the use of prime editing to correct HBBS by plasmid transfection in HEK293T cells containing the SCD mutation, reaching up to 58% efficiency (Fig. 1a)24. In contrast to HEK293T cells, HSPCs are difficult to transfect with plasmid DNA but are amenable…

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A ferritin-based COVID-19 nanoparticle vaccine that elicits robust, durable, broad-spectrum neutralizing antisera in non-human primates

IgG plasmids Antibody sequences and Fc-tagged ACE2 were cloned into the CMV/R plasmid backbone for expression under a CMV promoter. The antibodies with variable HC/LC were cloned between the CMV promoter and the bGH poly(A) signal sequence of the CMV/R plasmid to facilitate improved protein expression. The variable region was…

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Previously uncharacterized rectangular bacterial structures in the dolphin mouth

To maximize reproducibility, a list of the reagents and resources used in this study, as well as their source and identifier, is provided in Supplementary Table 5. Experimental model and subject details Oral swab samples were obtained from bottlenose dolphins (Tursiops truncatus) managed by the U.S. Navy MMP Biosciences Division, Space…

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Garden cress seeds ameliorated Aluminum-induced Alzheimers

Introduction Exposure of humans to aluminum has significantly increased worldwide. Potential sources of aluminum exposure include drinking water, food, medicines, vaccines, and aluminum cookware utensils. Aluminum bioaccumulation in the brain has been associated with neuroinflammatory and neurodegenerative changes that might predispose to Alzheimer’s disease (AD).1 Subchronic administration of Al3+ to rats…

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More than one proteins in one row in mass spectrometry data

More than one proteins in one row in mass spectrometry data 1 I have a matrix for whole cell mass spectrometry data. I found that many rows have more than one protein. I am new to proteomics. May I ask the reason behind this? proteomics mass_spectrometry • 63 views It…

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Uncovering biology by single-cell proteomics

To meet the challenge of sample depletion during preparation, there have been a number of recent advances in what is now a rapidly moving field of study of SCP, with important contributions from several investigators. Earlier work combined capillary electrophoresis (CE) with high-resolution mass spectrometry (HRMS) to analyze proteomes of…

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Revolutionizing viral disease vaccination: the promising clinical advancements of non-replicating mRNA vaccines | Virology Journal

To KK-W, Sridhar S, Chiu KH-Y, Hung DL-L, Li X, Hung IF-N, Tam AR, Chung TW-H, Chan JF-W, Zhang AJ-X, et al. Lessons learned 1 year after SARS-CoV-2 emergence leading to COVID-19 pandemic. Emerg Microbes Infect. 2021;10(1):507–35. Article  CAS  PubMed  PubMed Central  Google Scholar  Gao Q, Bao L, Mao H,…

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Creative BioMart Released New Safe and Traceable Recombinant GMP Proteins

PRESS RELEASE Published April 4, 2023 Creative BioMart GMP proteins, a sub-brand of Creative BioMart that provides comprehensive GMP proteins and peptides with defined identity, quality and purity, recently released new recombinant GMP proteins for in vitro diagnostics, therapeutic drug development, cosmetics, and the food industry. GMP grade protein is…

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Programmable protein delivery with a bacterial contractile injection system

Plasmid construction The PVCpnf structural and accessory region (pvc1-16) and payload and regulatory region (Pdp1, Pnf and regulatory genes PAU_RS16570-RS24015) were synthesized de novo (GenScript) and cloned into pAWP78 and pBR322 backbones, respectively. All manipulations involving payload and regulatory plasmids (pPayload) involved standard PCR amplification with Phusion Flash 2x Master…

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Formation of bridges and large cellular clumps in CHO-cell microcarrier cultures: Effects of agitation dimethyl sulfoxide and calf serum

Avgerinos GC, Drapeau D, Socolow JS, Mao J, Hsiao K and Broeze RJ (1990) Spin filter perfusion system for high density cell culture: production of recombinant urinary type plasminogen activator in CHO cells. Bio/Technology 8: 54–58. Article  CAS  Google Scholar  Bichay JT, Adams EG, Inch RW, Brewer JE and Bhuyan…

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Effects of various disaccharide adaptations on recombinant IgA1 production in CHO-K1 suspension cells

Plasmid construction The DNA sequence of the IgA variable region was obtained from a hybridoma cell line infused with immune cells from dengue-infected volunteers in Thailand was established by SPYMEG technology in a previous work (Setthapramote et al. 2012). The following primers were used to clone the variable region of…

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Single-Cell Proteomics Preparation for Mass Spectrometry Analysis Using Freeze-Heat Lysis and an Isobaric Carrier

In this protocol, we describe how to prepare mammalian cells for single-cell proteomics analysis via mass spectrometry using commercially available reagents and equipment, with options for both manual and automatic pipetting. SCoPE2 can quantify thousands of proteins from thousands of single mammalian cells without antibodies. This protocol can help uncover…

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Vaccines | Free Full-Text | Immunogenicity of COVID-eVax Delivered by Electroporation Is Moderately Impacted by Temperature and Molecular Isoforms

Vaccines 2023, 11(3), 678; doi.org/10.3390/vaccines11030678 (registering DOI) Vaccines 2023, 11(3), 678; doi.org/10.3390/vaccines11030678 (registering DOI) Received: 6 February 2023 / Revised: 7 March 2023 / Accepted: 10 March 2023 / Published: 16 March 2023 Round 1 Reviewer 1 Report (Previous Reviewer 1) To Authors: The manuscript “Immunogenicity of COVID-eVax delivered by electroporation is…

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sygrna – Bjavkoreannew

SygRNA Cas9 Synthetic Modified tracrRNA; HPLC-purified SygRNA modified tracrRNA oligo is optimized for use with a target-specific crRNA or modified crRNA …The SygRNA™ synthetic RNA system recruits SpCas9 nuclease to specific genomic targets. The tracrRNA (trans-activating crRNA) binds to a crRNA (CRISPR RNA) …16 nov. 2016 — SygRNA™-Synthetic Two Part CRISPR RNA…

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Emerging Applications of Oligonucleotide Synthesis: Current Scenario and Future Prospects

Oligonucleotide Synthesis Market Oligonucleotides, also known as oligomers, are small DNA or RNA molecules that have a variety of uses in genetic testing, research, and forensics. PORTLAND, OREGON, UNITED STATES, March 6, 2023 /EINPresswire.com/ — Oligonucleotide synthesis is the process of chemically synthesizing short strands of DNA or RNA molecules….

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New workflow for the analysis of mRNA therapeutics

Here, Mark Dickman, Professor in Bioanalytical Science and Engineering at the University of Sheffield, explores how analytical techniques such as liquid chromatography-mass spectrometry can support the manufacturing of mRNA therapeutics. Large RNA is rapidly emerging as a new class of therapeutics. Nowhere is this more publicly evident than in the development, regulatory…

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A bivalent remipede toxin promotes calcium release via ryanodine receptor activation

Recombinant peptide production Recombinant expression of Xt3a, Xt3a-D1, Xt3a-D2 and IpTxA was performed using an E. coli expression system. A gene encoding the peptide was subcloned into an expression vector containing a coding region with poly-histidine purification tag as well as a solubility tag (MBP for Xt3a and SUMO for…

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Engineering protein glycosylation in CHO cells to be highly similar to murine host cells

1 Introduction Recombinant therapeutic proteins, also known as biologics, have been successfully used to treat a wide range of diseases, including cancers, inflammatory disorders, and cardiovascular diseases (Walsh, 2018). Biosimilars with comparable efficacy and safety profiles have emerged as alternative options to innovator therapies. A biosimilar is a biologic that…

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Polydopamine-Liposome as Versatile Theranostics Nanoplatform

Introduction According to the global cancer statistics 2018, breast cancer has the highest global incidence (28.9%) and mortality rates (23.0%) in women.1 Chemotherapy, as a mainstay in clinical breast cancer treatment, is limited by severe side-effects resulting from the nonspecific drug distribution in the human body.2–4 What is worse, a…

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A universal CRISPR/Cas12a-powered intelligent point-of-care testing platform for multiple small molecules in the healthcare, environment, and food

Growing studies focusing on nuclear acid detection via the emerging CRISPR technique demonstrate its promising application. However, limited works solve the identification of non-nucleic acid targets, especially multiple small molecules. To address challenges for point-of-care testing (POCT) in complex matrices for healthcare, environment, and food safety, we developed CRISPR Cas12a-powered…

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siRNA Synthesis Overview | Life Science & Biotechnology Laboratory Singapore

Overview siRNA synthesis has been globally recognized as one of the most effective ways for the silencing of target genes in cells or body, and is widely accepted due to its low cost and high transfection efficiency. The well-known siRNA synthesis is not only the optimum method for RNAi‐based clinical…

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