Tag: HTSlib
Ubuntu Manpage: samtools targetcut – cut fosmid regions (for fosmid pool only)
Provided by: samtools_1.13-2_amd64 NAME samtools targetcut – cut fosmid regions (for fosmid pool only) SYNOPSIS samtools targetcut [-Q minBaseQ] [-i inPenalty] [-0 em0] [-1 em1] [-2 em2] [-f ref] in.bam DESCRIPTION This command identifies target regions by examining the continuity of read depth, computes haploid consensus sequences of targets and…
Detailed differences between sambamba and samtools
3 month , My first post in the new student group , The false-positive mutation appears because duplicates mark Not enough ?, Tells the story of supplementary read It won’t be GATK MarkDuplicates Marked as duplicates The problem of . after , In response to this question , I began…
bam – samtools view command not found error
When I tried to use samtools to split a bam file based on different chromosomes, I used this command: samtools view input.bam -b chr21 | chr21.bam However, I get error messages like this: -bash: chr21.bam: command not found [W::hts_idx_load3] The index file is older than the data file: input.bam.bai How…
JUWELSBOOSTER module browser
HTSlib Compiler/GCCcore/11.2.0 1.14 Description =========== A C library for reading/writing high-throughput sequencing data. This package includes the utilities bgzip and tabix More information ================ – Homepage: www.htslib.org/ – Site contact: Support <sc@fz-juelich.de>, software installed by Alexandre Strube <a.strube@fz-juelich.de> EBROOTHTSLIB /p/software/juwelsbooster/stages/2022/software/HTSlib/1.14-GCCcore-11.2.0 EBVERSIONHTSLIB 1.14 EBDEVELHTSLIB /p/software/juwelsbooster/stages/2022/software/HTSlib/1.14-GCCcore-11.2.0/easybuild/Compiler-GCCcore-11.2.0-HTSlib-1.14-easybuild-devel +CMAKE_PREFIX_PATH /p/software/juwelsbooster/stages/2022/software/HTSlib/1.14-GCCcore-11.2.0 +CPATH /p/software/juwelsbooster/stages/2022/software/HTSlib/1.14-GCCcore-11.2.0/include +LD_LIBRARY_PATH /p/software/juwelsbooster/stages/2022/software/HTSlib/1.14-GCCcore-11.2.0/lib…
sam – Use Htslib to create auxilary tags in bam file C++
I am creating a threaded c++ file where i generate in silico bam files, using header, DNA sequence and read information. First i use bam_init1() to create the bam1_t structure just named “b”. Then i use bam_set1 to create the actual sequence entry in the bam file bam_set1(b,read_id_length,READ_ID,flag,chr_idx,min_beg,mapq,n_cigar,cigar,-1,-1,0,strlen(DNAsequence),DNAsequence,quality_string,l_aux) And finally…
bioconductor – Trouble installing Rhtslib in R/R studio
I’m using RStudio on Ubuntu 18 and I’m trying to install the htslib package from the Bioconductor repo, but I’m stuck now. This is what I get: * installing *source* package ‘Rhtslib’ … ** using non-staged installation via StagedInstall field ** libs cd “htslib-1.7” && make -f “/usr/lib/R/etc/Makeconf” -f “Makefile.Rhtslib”…
Ubuntu Manpage: samtools reheader – replaces the header in the input file
Provided by: samtools_1.13-2_amd64 NAME samtools reheader – replaces the header in the input file SYNOPSIS samtools reheader [-iP] [-c CMD | in.header.sam ] in.bam DESCRIPTION Replace the header in in.bam with the header in in.header.sam. This command is much faster than replacing the header with a BAM→SAM→BAM conversion. By default…
Fail to install Ensembl VEP
Fail to install Ensembl VEP 2 Hello I have been encounter some issues on installing the Ensembl VEP. I am using Ubuntu 20.04 system, with the newest perl v5.34. perl -MBio::Root::Version -e ‘print $Bio::Root::Version::VERSION,”n”‘ 1.006924 However, when I install the VEP, it failed. Hello! This installer is configured to install…
Running samtools view on bam affects the number of variants called by both haplotypecaller and deepvariant – C samtools
Thanks for getting back to me Valeriu. As you suggested, I used the latest commit from the develop branch in my pipeline, and the results look good. I was able to replicate the numbers from samtools v1.10.2 and v1.11 for both variant callers. FYI $ docker run scilifelabram/htslib:dev_proper /opt/samtools/samtools version…
Access read group tag for BAM reads – rust-htslib
I’m sure this is probably straightforward, but I can’t figure out how to access the read group information on a per-read basis within a BAM file. I can get it from the header, but I can’t determine how to use aux or any other record method to get that info….
kojix2/ruby-htslib: v0.0.4 | Zenodo
Zenodo DOI Badge DOI 10.5281/zenodo.5797825 Markdown [](https://doi.org/10.5281/zenodo.5797825) reStructedText .. image:: zenodo.org/badge/DOI/10.5281/zenodo.5797825.svg :target: doi.org/10.5281/zenodo.5797825 HTML <a href=”https://doi.org/10.5281/zenodo.5797825″><img src=”https://zenodo.org/badge/DOI/10.5281/zenodo.5797825.svg” alt=”DOI”></a> Image URL zenodo.org/badge/DOI/10.5281/zenodo.5797825.svg Target URL doi.org/10.5281/zenodo.5797825 Read more here: Source link
Issue with installing QIIME2 2021.11 on Windows 10 – Technical Support
Hi QIIME support team, I’m attempting to install QIIME2 on my Windows 10 machine. I installed Anaconda3, then set up conda to run in Git Bash: echo “. ${PWD}/conda.sh” >> ~/.bashrc Once I restarted Git Bash and activated Conda, I installed python-wget because installation of wget kept getting the following…
Issues installing Pindel from git
Issues installing Pindel from git 1 I’m currently attempting to install Pindel on my ubuntu machine (16.04 server), but have been having issues with running the ./INSTALL file. The ./INSTALL file takes the path to htslib as an argument, so I also installed htslib from git with git clone github.com/samtools/htslib…
cellranger count DETECT_COUNT_CHEMISTRY (failed)
cellranger count DETECT_COUNT_CHEMISTRY (failed) 0 I am learning scRNA-seq and the tutorial I follow uses dataset (1k pbmcs from healthy donor) from 10X genomics website. I downloaded fastq and reference transcriptome files and ran following command. cellranger-6.1.1/cellranger count –id pbmc_1k_v2_example –transcriptome /home/murat/Share/single_cell/refdata-gex-GRCh38-2020-A –fastqs /home/murat/Share/single_cell/pbmc_1k_v2_fastqs I get following message. Martian Runtime…
plotting roh from bcftools
plotting roh from bcftools 0 Heys, I am following this small tutorial on how to calculate ROHs from a vcf file using bcftools (samtools.github.io/bcftools/howtos/roh-calling.html) and I am getting this txt file: # This file was produced by: bcftools roh(1.10.2+htslib-1.10.2-3) # The command line was: bcftools roh -G30 –AF-dflt 0.4 my_file.vcf…
User friendly (visual&interactive) VCF/BCF mining tools (2021)
What is currently the best user friendly (visual and interactive) VCF/BCF mining tool in 2021? For VCF/BCF similar to size or even larger than the 1000 human genomes VCF? I guess most organization do not have a visual and interactive mining VCF mining tool but use either: A website front-end…
tabix for ID column
tabix for ID column 4 Hello, I’m looking for something similar to tabix. But instead of looking for informations within a given region, I would like to use the values in the ID column for quickly lookup. So for example I would like to take the compressed dbSNP file, index…
Bam indexing error-the samtools index command for just 1 chromosome is also not working in this case, the following error is showing [E::hts_idx_push] Region 536871288..536871299 cannot be stored in a bai index. Try using a csi index with min_shift = 14, n_lvls >= 6 samtools index: failed to create index for P2R1R2_aln_sorted.bam”: Numerical result out of range.
Ask questionsBam indexing error-the samtools index command for just 1 chromosome is also not working in this case, the following error is showing [E::hts_idx_push] Region 536871288..536871299 cannot be stored in a bai index. Try using a csi index with min_shift = 14, n_lvls >= 6 samtools index: failed to create…
install GenomicFeatures fail
install GenomicFeatures fail 1 @5b9023e7 Last seen 19 hours ago China BiocManager::install(‘GenomicFeatures’) results show ‘getOption(“repos”)’ replaces Bioconductor standard repositories, see ‘?repositories’ for details replacement repositories: CRAN: mirrors.tuna.tsinghua.edu.cn/CRAN/ Bioconductor version 3.14 (BiocManager 1.30.16), R 4.1.0 (2021-05-18) Installing package(s) ‘GenomicFeatures’ also installing the dependencies ‘Rhtslib’, ‘Rsamtools’, ‘GenomicAlignments’, ‘rtracklayer’ Packages which are only…
bcftools merge; retaining sample names
bcftools merge; retaining sample names 2 When I do bcftools merge, the headers do not retain the filenames. How can I specify filenames? This is my command bcftools merge vcf/unfiltered/*.vcf.gz -O z > msa/pooled.vcf.gz However this is the relevant part of my header, despite the filenames I gave it. Is…
Disappearing CB, the bam tag after samtools sort -t CB
I’ve been trying to setup an analysis pipline for RNAvelocity in AWS EC2. I used one of the 10x dataset, 10k Peripheral blood mononuclear cells (PBMCs) from a healthy donor, Single Indexed, as a test model to setup the pipeline. For speed and cost saving, I first used samtools…
What does samtools mean by ‘orientation’ when marking duplicates?
What does samtools mean by ‘orientation’ when marking duplicates? 0 Hello Biostars, It is my understanding that samtools marks duplicates on the basis of the 5′ position of reads and also the orientation of reads. This is based on my reading of the following: www.htslib.org/algorithms/duplicate.html However, I am not sure…