Tag: iso-seq

Single-cell Iso-Sequencing enables rapid genome annotation for scRNAseq analysis

. 2022 Mar 3;220(3):iyac017. doi: 10.1093/genetics/iyac017. Affiliations Expand Affiliations 1 Institute of Ecology and Evolution, University of Oregon, Eugene, OR 97403, USA. 2 Presidential Initiative in Data Science, University of Oregon, Eugene, OR 97403, USA. Item in Clipboard Hope M Healey et al. Genetics. 2022. Show details Display options Display options…

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What are the steps of an full lengh RNA seq annotation method?

What are the steps of an full lengh RNA seq annotation method? 0 I have read a few papers, some of them used GenMark, or PacBios proprietary Iso-Seq pipeline but as I’m just a Bachelor’s student I am having trouble understanding how exactly it works. Can some one roughly explain…

Continue Reading What are the steps of an full lengh RNA seq annotation method?

How does PacBio Iso-Seq annotation compare to other pipelines?

How does PacBio Iso-Seq annotation compare to other pipelines? 1 I have no experience in annotation RNA seq data (or genome assembly) but I’ve spent the last weeks ploughing through some papers and looking at manuals. I am wondering why it seems so common that e.g. proprietary pipelines of sequencers…

Continue Reading How does PacBio Iso-Seq annotation compare to other pipelines?

How does PacBio Iso -Seq Annotation compare to other pipelines?

How does PacBio Iso -Seq Annotation compare to other pipelines? 1 I have no experience in annotation or assembly but I’ve spent the last weeks ploughing through some papers and looking at manuals. I am wondering why it seems so common that e.g. proprietary pipelines of sequencers are not used?…

Continue Reading How does PacBio Iso -Seq Annotation compare to other pipelines?