Tag: IVT

Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair

Khan, S. et al. Role of recombinant DNA technology to improve life. Int. J. Genomics Proteomics 2016, 2405954 (2016). Google Scholar  Spolski, R., Li, P. & Leonard, W. J. Biology and regulation of IL-2: from molecular mechanisms to human therapy. Nat. Rev. Immunol. 18, 648–659 (2018). Article  CAS  PubMed  Google…

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A Step Forward In Vaccine Technology: Exploring The Effects Of N1-methylpseudouridine In MRNA Translation

In a recent study published in Nature, a group of researchers explored how the incorporation of N1-methylpseudouridine (1-methylΨ) into messenger ribonucleic acids (mRNAs) affects ribosomal frameshifting and the overall fidelity of mRNA translation. Study: N1-methylpseudouridylation of mRNA causes +1 ribosomal frameshifting. Image Credit: MattL_Images/Shutterstock.com Background  Therapeutic in vitro-transcribed (IVT) mRNAs,…

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Common analysis of direct RNA sequencinG CUrrently leads to misidentification of m5C at GCU motifs

Introduction Oxford Nanopore Technologies (ONT) direct RNA sequencing (Fig 1A) enables detection of RNA modifications. A modified base produces an altered electrical current and/or dwell time relative to a canonical base that can be detected with algorithms (Garalde et al, 2018; Smith et al, 2019; Workman et al, 2019). Figure…

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ST Pharm Presented ‘100-Day Expedite Strategy’, Preparedness and Proactive Measures for Future Pandemic at Korea-CEPI R&D Workshop

SEOUL, South Korea, Nov. 20, 2023 /PRNewswire/ — Nov. 21, 2023 local time, ST Pharm announced ‘Rapid Preparedness of Pandemic Vaccine in 100 Days using ST Pharm’s own mRNA Platform Technology’ at the Korea-CEPI R&D Workshop in World Bio Summit 2023, organized by Korea Health Industry Development Institute (KHIDI) and Coalition…

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Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B | BMC Microbiology

Clinical samples Thirty nasopharyngeal aspirates were collected from individuals with symptoms of respiratory tract infections, and tested positive for HRV-A (5 clinical samples), HRV-B (20 clinical samples), or HRV-C-positive (5 clinical samples) based on the method, as previously described [27]. Ten other specimens were tested negative for HRV-B, including human…

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Are Residual DNA In mRNA Vaccines Dangerous?!

Are residual DNA impurities in mRNA vaccines dangerous, and can they integrate into our DNA?! Take a look at the viral claims, and find out what the facts really are!   Claim : Residual DNA In mRNA Vaccines Are Dangerous! Anti-vaccine activists are sharing and/or promoting an article by The…

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Targeting nucleic acid phase transitions as a mechanism of action for antimicrobial peptides

Phase separation propensity of AMPs We retrieved the sequences of previously identified AMPs deposited into the Database of Antimicrobial Activity and Structure of Peptides (DBAASP)40 and in the APD3 antimicrobial peptide database41 to analyse their predicted propensities to undergo phase separation. Interestingly, we find that some AMPs possess features42,43,44 that…

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Did EMA Confirm Pfizer Hid SV40 DNA In Vaccine?!

Did the EMA (European Medicines Agency) just confirm that Pfizer hid the presence of SV40 DNA in its mRNA COVID-19 vaccine? Take a look at the viral claim, and find out what the facts really are!   Claim : EMA Confirmed Pfizer Hid SV40 DNA In Vaccine! Some people are…

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Are mRNA Vaccines Contaminated With SV40 DNA?!

Are mRNA COVID-19 vaccines from Pfizer and Moderna contaminated with SV40 virus DNA that can cause turbo cancer and other health problems?! Take a look at the viral claims, and find out what the facts really are!   Claim : mRNA Vaccines Are Contaminated With SV40 DNA! Some people are…

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How mRNA technology is creating affordable, potent vaccine products

BPR: What are the advantages of RNA medicine?​ The pace, efficacy and scalability with which the success of mRNA vaccines against COVID-19 was demonstrated opened endless possibilities for mRNA medicine in broad areas of infectious diseases, cancers and protein-encoding replacement therapies. In my mind, the advantages of mRNA medicines are…

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Did Health Canada Confirm SV40 DNA In Pfizer Vaccine?!

Did Health Canada just confirm the presence of SV40 DNA in the Pfizer mRNA vaccine for COVID-19?! Take a look at the viral claim, and find out what the facts really are!   Claim : Health Canada Confirmed SV40 DNA In Pfizer Vaccine! Some people are sharing an article by…

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Identification of CCZ1 as an essential lysosomal trafficking regulator in Marburg and Ebola virus infections

Cells and viruses Haploid mSCs AN3–12 are a feeder independent clonal derivative of HMSc2 isolated from mice oocyte and maintained at IMBA52. The AN3–12 library and knocked out cells used for the haploid screening was obtained from IMBA (Austria)14,52. AN3–12 cells were validated by STR analysis. Haploid mES cells were…

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Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality

Experimental design Primary T cell isolation, expansion, and cell culture Primary T cells were isolated from human peripheral blood mononuclear cells (PBMCs) of healthy donors collected by the Institute for Transfusion Medicine of the University Clinic of Leipzig, Germany, approved by the Ethics Committee of the Faculty of Medicine of…

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Pfizer and Moderna Covid mRNA Injections Include DNA Fragments

It has been just revealed the fact that a new study has found the “presence of billions to hundreds of billions of DNA molecules per dose” in both Pfizer and Moderna mRNA injections. Check out more details below. Covid vaccines in the news again It has been just revealed the…

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GenScript hiring Senior Research Scientist, Bioinformatics (Greater Seattle, WA) in United States

About GenScriptGenScript Biotech Corporation (Stock Code: 1548.HK) is a global biotechnology group. Founded in 2002, GenScript has an established global presence across North America, Europe, the Greater China, and Asia Pacific. GenScript’s businesses encompass four major categories based on its leading gene synthesis technology, including operation as a Life Science…

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Rapid, tunable, and multiplexed detection of RNA using convective array PCR

Integrating reverse transcription in caPCR We first introduced an RT step to the standard caPCR assay. caPCR is performed using a set of 4 heaters that clamp on either face of a cartridge containing a toroidal chamber filled with the PCR reaction mixture4. By setting heaters on one side of…

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Rapid Detection of blaKPC in Carbapenem-Resistant Enterobacterales Based on CRISPR/Cas13a

Materials and Instruments Clinical Strains and Sputum Samples Collection A total of 117 bacterial strains were isolated and collected from sputum samples of individual patients with suspected intestinal infection treated at our hospital during the first half of 2021. Carbapenemase production was confirmed by the Modified Carbapenem Inactivation Method (mCIM)…

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mRNA vaccine quality analysis using RNA sequencing

Design and synthesis of reference plasmid A reference construct was first designed, with the intention of optimising the production of RNA therapeutics for pre-clinical research. The coding sequence of eGFP30 was selected as a reporter in the coding region, as its protein product can be assayed simply through Flow cytometry…

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Stem Cells and Regenerative Medicine: A Journey into Quality and Consistency

Key Takeaways: Induced pluripotent stem cells (iPSCs) are the future of regenerative medicine, but quality and consistency are paramount mRNA-based reprogramming offers higher efficiency and safety for iPSC generation than DNA-based reprogramming Ensuring consistent quality in iPSC-derived cell therapy requires a comprehensive approach that includes core technology, GMP compliance, good…

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Technologies | Abnova

Wheat Germ Protein Expression Proximity Ligation Assay Mouse Monoclonal Antibody Production Rabbit Monoclonal Antibody (Phage Antibody Library) Chimera RNAi Fluorescence In Situ Hybridization (FISH) Membrane Protein Proteoliposomes Protein and Post-Translational Modification Quantification ACTN4 FISH Probe CytoQuest™ CR LiquidCell™ Cell-Surface Vimentin (CSV) Monoclonal Antibody mutaFISH™ (mutation-specific Fluorescence In Situ Hybridization) Next-Generation Sequencing DNAx™ Immune SAMx™ Immune COVID-19 Humanized Monoclonal Antibody COVID-19 SAM Universal Vaccine COVID-19 circRNA Vaccine Omicron…

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GenScript Adds Circular RNA, Lipid Nanoparticles Formulation

GenScript Biotech Corporation, a provider of life-science research tools and services, expanded its IVT mRNA synthesis portfolio with the addition of customized circular RNA (circRNA) and lipid nanoparticles (LNPs) formulation services. The new contract-manufactured reagent services are available immediately to provide reagents that advance research in fields such as vaccine…

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GenScript Expands Range of Reagent Services, Adding Circular RNA and Lipid Nanoparticles Formulation

Building on GenScript’s fast and dependable in-vitro transcription mRNA synthesis service, GenScript now offers the most comprehensive range of RNA formats critical, enabling fast delivery of complex design for the research and development of RNA vaccines and therapeutics PISCATAWAY, N.J., Sept. 18, 2023 /PRNewswire/ — GenScript Biotech Corporation, the world’s…

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Guidelines For mRNA Drug Product Manufacturing And Quality Control

By John F. Cipollo, United States Pharmacopeia Messenger RNA (mRNA) is the template for protein synthesis. It serves as an intermediate to transfer the genetic information from DNA in the nucleus to the ribosome sites for translation to protein. It can encode virtually any protein, making it extremely attractive as…

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Avirmax Inc. Will Attend the BioProcess International Meeting in Boston

HAYWARD, Calif., Sept. 13, 2023 /PRNewswire/ — Avirmax Inc., Vice President of Research, Dr. Li Ou, and Associate Director of Operations, Ms. June Song, will attend the BioProcess International meeting in Boston between September 18th to 21st, 2023. As an Associate Sponsor, Avirmax will have a booth (#223) during the exhibit…

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High-Efficiency Gene Disruption in Primary Bone Marrow-Derived Macrophages Using Electroporated Cas9-sgRNA Complexes

The IVT template is a 127 bp PCR product (Figure 1B). The full-length IVT product is a 98 nt RNA, which migrates similarly to a 70 bp double-stranded DNA fragment (Figure 1C). After electroporation, the cells should be >90% viable, with a total cell count of >70% of the starting…

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Bioinformatics job with Seqirus | 1401880040

The Opportunity: You will be responsible for the in vitro sa-mRNA synthesis, encapsulation and characterization methods. The scope of work will include the design of drug substance manufacturing process, close collaboration with purification and drug product development teams. Cross functional collaborations with the analytical, manufacturing and research teams are also…

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Applied DNA buys Spindle Biotech to improve mRNA production offering

Applied DNA Sciences has acquired Spindle Biotech to improve its mRNA manufacturing capabilities and provide a more efficient production platform. Applied DNA identified the proprietary RNA polymerase (RNAP) that Spindle is commercializing as complementary to its existing LinearDNA platform, leading it to buy the startup for $625,000 in cash and…

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Applied DNA Sciences Acquires Spindle Biotech, Launches LineaIVT Platform

NEW YORK – Applied DNA Sciences announced on Thursday that it acquired RNA polymerase developer Spindle Biotech. The acquisition enables Applied DNA to combine its LinearDNA IVT platform with Spindle’s proprietary, high-performance RNA polymerase (RNAP) into an integrated platform called LineaIVT, for which Applied DNA also launched an early access…

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Cell-free synthetic DNA in mRNA manufacturing

By Ashish Dhir, PhD and Amy Walker, PhD, 4basebio The pace, efficacy and scalability with which the success of mRNA vaccines against COVID-19 was demonstrated opened endless possibilities for mRNA medicine in broad areas of infectious diseases, cancers, protein-encoding replacement therapies.  The advantages of RNA medicine are obvious, with the…

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Bioinformatics job with Seqirus | 1401863425

The Opportunity: You will be responsible for the in vitro sa-mRNA synthesis, encapsulation and characterization methods. The scope of work will include the design of drug substance manufacturing process, close collaboration with purification and drug product development teams. Cross functional collaborations with the analytical, manufacturing and research teams are also…

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mRNA Vaccines for Rapid Response to Biological Threats

COVID-19 demonstrated the enormous dangers posed by biological threats, severely impacting virtually every community in the world. Mitigating these risks in the future is an urgent global imperative. To that end, the National Biodefense Strategy and Implementation Plan, published by the U.S. government in October 2022, outlines goals for countering…

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New Products Posted to GenomeWeb: Bionano Genomics, Devyser, Eurofins Genomics, Revvity/Honeycomb

Bionano Genomics Stratys System, Via Software Bionano Genomics has launched the Stratys system for high-throughput optical genome mapping and the Via software for analyzing genomic data for hematologic malignancies. The Stratys instrument will have up to fourfold greater data output than the current Saphyr platform. It enables the use of…

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Eurofins Genomics Blue Heron launches IVT mRNA Synthesis Service: The Future of Gene Expression

BOTHELL, Wash., June 29, 2023 /PRNewswire/ — Eurofins Genomics Blue Heron is thrilled to announce the launch of our cutting-edge IVT mRNA Synthesis Service. With this revolutionary service, we aim to offer a game-changing solution to the challenges of gene expression research. The new service offers a comprehensive solution for…

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Eurofins Genomics Blue Heron Launches IVT MRNA Synthesis Service

Eurofins Genomics Blue Heron, a provider of DNA synthesis, sequencing services, and gene synthesis, launched its cutting-edge IVT mRNA Synthesis Service. With the service, the company aims to offer a game-changing solution to the challenges of gene expression research. The new service offers a comprehensive solution for researchers seeking high-quality,…

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Beacon Therapeutics to develop gene therapies for retinal diseases

Beacon Therapeutics Holdings Limited has been launched to develop gene therapies to treat a range of rare and prevalent retinal diseases that result in blindness.  Syncona Limited, with additional investors including OSE, has provided £96 million ($120 million) to fund the acquisition of AGTC and provide capital to take Beacon…

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Cell rejuvenation for age-related diseases

By Sharon Rosenzweig-Lipson, CSO, Life Biosciences Cellular rejuvenation is quickly becoming a hot topic in the development of novel therapeutic modalities for a range of diseases.  The leading approaches to cellular rejuvenation currently in development utilize epigenetic reprogramming to achieve this goal. Epigenetics is the study of how positive and…

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Peptide-encoding mRNA barcodes for the high-throughput in vivo screening of libraries of lipid nanoparticles for mRNA delivery

We reasoned that an ideal screening system for functional mRNA delivery would be model independent so that it could be applied in any preclinical model of disease, would consist of multiple measures of protein production that are each orthogonal to any others such that multiple formulations could be tested within…

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Revolutionizing viral disease vaccination: the promising clinical advancements of non-replicating mRNA vaccines | Virology Journal

To KK-W, Sridhar S, Chiu KH-Y, Hung DL-L, Li X, Hung IF-N, Tam AR, Chung TW-H, Chan JF-W, Zhang AJ-X, et al. Lessons learned 1 year after SARS-CoV-2 emergence leading to COVID-19 pandemic. Emerg Microbes Infect. 2021;10(1):507–35. Article  CAS  PubMed  PubMed Central  Google Scholar  Gao Q, Bao L, Mao H,…

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Taking siRNA Treatments Beyond the Liver

An inside view on a platform that could help take RNA therapeutics to the next level of delivery Arthur T Suckow | 03/21/2023 | 5 min read It was during my PhD in Pharmacology at UC San Diego that I found my desire to launch a biotech company. Amazed by…

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Hydrogels for RNA delivery | Nature Materials

Yin, H. et al. Non-viral vectors for gene-based therapy. Nat. Rev. Genet. 15, 541–555 (2014). Article  CAS  Google Scholar  Wu, S. Y., Lopez-Berestein, G., Calin, G. A. & Sood, A. K. RNAi therapies: drugging the undruggable. Sci. Transl. Med. 6, 240ps7 (2014). Article  Google Scholar  Mendes, B. B. et al….

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Likang gets trial ok for China’s first personalized cancer vaccine

Likang Life Sciences has received implied approval for the clinical trial of its personalized neoantigen-targeted vaccine for advanced solid tumors, LK101 injection.  This is the first personalized neoantigen vaccine and mRNA editing product approved by China’s Center for Drug Evaluation (CDE) of the National Medical Products Administration (NMPA) to enter…

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Phase 3 Study Demonstrates Comparable Safety, Efficacy Between Ranibizumab Biosimilar, Lucentis for nAMD

Proposed biosimilar FYB201 is considered biosimilar to reference ranibizumab (Lucentis) in terms of clinical efficacy and safety in the treatment of patients with newly diagnosed subfoveal neovascular age-related macular degeneration (nAMD), and may be a new patient treatment option, according to a study published in Ophthalmology. This trial, called the…

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New workflow for the analysis of mRNA therapeutics

Here, Mark Dickman, Professor in Bioanalytical Science and Engineering at the University of Sheffield, explores how analytical techniques such as liquid chromatography-mass spectrometry can support the manufacturing of mRNA therapeutics. Large RNA is rapidly emerging as a new class of therapeutics. Nowhere is this more publicly evident than in the development, regulatory…

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sgRNA Synthesis Kit – Labshake

No products found because this supplier’s products are not listed. Arpita Sarkar, Sophie Lanciano, Gael Cristofari, bioRxiv – Genomics 2022 Quote: EnGen sgRNA synthesis kit (NEB) No products found because this supplier’s products are not listed. Jitendra S. Kanshana, et al., bioRxiv – Genetics 2021 Quote: … A PCR-generated sgRNA…

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Interpreting the meaning of outward facing reads

Interpreting the meaning of outward facing reads 0 Sorry i have lots of data and im not sure how to interpret. I took mRNA created by IVT from our plasmid and performed paired end Illumina sequencing.I used samtools stats to get info about the various samples: read length ~150, insert…

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The Promise of RNA-Based Therapies

Published a day ago Submitted by CRB Decades of scientific progress in understanding the root cause of many formerly intractable diseases has opened up new treatment options, namely through RNA-based therapies. This second wave of biopharma, in which our understanding of the ways coding portions of our genome relate to…

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Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts

We designed pDOC to support multiple strategies for analyzing protein degradation in vitro, specifically cell-free systems. The device is based on a MITOMI integrated microfluidic chip, originally developed to quantify protein-ligand interactions at equilibrium24,33. The MITOMI module was modified to contain an array of 16 × 64, 32 × 32…

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CRISPR-SpRYgests Enable Precise Cleavage of DNA Bases In Vitro

NEW YORK — A CRISPR-Cas variant engineered to no longer need a protospacer adjacent motif (PAM) can be harnessed to make cuts at any DNA base in vitro, a new study reports. Its ability to make precise breaks anywhere could be applied to a number of DNA engineering applications. Researchers…

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4basebio Plc – Half-year Report

4basebio PLC(“4basebio”, the “Company” or the “Group”) Half-year Reportfor the six months ended 30 June 2022 4basebio PLC (AIM: 4BB), the specialist life sciences group focused on exploiting intellectual property in the field of cell and gene therapies and DNA vaccines, announces its unaudited half-year results for the six months…

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Challenges of single-cell and single-nucleus RNA-sequencing from core needle biopsy specimens

Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA-seq (snRNA-seq) allow transcriptomic profiling of thousands of cells from a renal biopsy specimen at a single-cell resolution. Both methods are promising tools to unravel the underlying pathophysiology of glomerular diseases. KU Leuven researchers provide an overview of the technical challenges that should be…

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