Tag: IVT

Interpreting the meaning of outward facing reads

Interpreting the meaning of outward facing reads 0 Sorry i have lots of data and im not sure how to interpret. I took mRNA created by IVT from our plasmid and performed paired end Illumina sequencing.I used samtools stats to get info about the various samples: read length ~150, insert…

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The Promise of RNA-Based Therapies

Published a day ago Submitted by CRB Decades of scientific progress in understanding the root cause of many formerly intractable diseases has opened up new treatment options, namely through RNA-based therapies. This second wave of biopharma, in which our understanding of the ways coding portions of our genome relate to…

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Affinity microfluidics enables high-throughput protein degradation analysis in cell-free extracts

We designed pDOC to support multiple strategies for analyzing protein degradation in vitro, specifically cell-free systems. The device is based on a MITOMI integrated microfluidic chip, originally developed to quantify protein-ligand interactions at equilibrium24,33. The MITOMI module was modified to contain an array of 16 × 64, 32 × 32…

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CRISPR-SpRYgests Enable Precise Cleavage of DNA Bases In Vitro

NEW YORK — A CRISPR-Cas variant engineered to no longer need a protospacer adjacent motif (PAM) can be harnessed to make cuts at any DNA base in vitro, a new study reports. Its ability to make precise breaks anywhere could be applied to a number of DNA engineering applications. Researchers…

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4basebio Plc – Half-year Report

4basebio PLC(“4basebio”, the “Company” or the “Group”) Half-year Reportfor the six months ended 30 June 2022 4basebio PLC (AIM: 4BB), the specialist life sciences group focused on exploiting intellectual property in the field of cell and gene therapies and DNA vaccines, announces its unaudited half-year results for the six months…

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Challenges of single-cell and single-nucleus RNA-sequencing from core needle biopsy specimens

Single-cell RNA sequencing (scRNA-seq) and single-nucleus RNA-seq (snRNA-seq) allow transcriptomic profiling of thousands of cells from a renal biopsy specimen at a single-cell resolution. Both methods are promising tools to unravel the underlying pathophysiology of glomerular diseases. KU Leuven researchers provide an overview of the technical challenges that should be…

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