Tag: LAMP

Cell lines and culture A549-hACE2 [a gift from Dr. Ben tenOever3], 293 T, HEK293, A549, and 3T3-L1 pre-adipocyte cell lines (refer to Supplementary Table 1) were cultured in DMEM and 10% FBS. U937, Calu-3, and HCT116 cells were cultured in RPMI, EMEM, and McCoy’s 5 A media, respectively, with 10% FBS. Caco-2 cells…

The epidermal growth factor receptor (EGFR), a member of the epidermal growth factor receptor (HER) family, is closely related to the activation of cell signaling pathways, including cell proliferation and apoptosis protection [1], [2]. Studies have been proved that EGFR is over-expressed in various tumors, especially in lung adenocarcinoma. This…

How about a well-researched study on the DNA/RNA Extraction Kit that includes an in-depth review of different patterns, programmes, and goods that have the potential to cause a paradigm shift in the growth rate? This is a reality. Based on the latest changes in the DNA/RNA Extraction Kit, Market Reports…

Introduction Phytophthora sojae is among the most destructive pathogens of soybean (Glycine max). An estimated annual worldwide loss of US$1–2 billion is caused by P. sojae, which was first identified as a soybean pathogen in the 1950s in Indiana, Ohio, and North Carolina (Schmitthenner, 1985; Tyler, 2007; Dorrance, 2018). The…

This protocol describes the fabrication and characterization of a photoresponsive prodrug-dye nanoassembly. The methodology for drug release from the nanoparticles by light-triggered disassembly, including the light irradiation setup, is explicitly described. The drugs released from the nanoparticles following light irradiation exhibited excellent anti-proliferation effects on human colorectal tumor cells. This…

Product Code Does a number code printed on a streetlight match that of a ribosomal protein and does this show that mRNA from the COVID-19 vaccines can be activated using optogenetic technology if a vaccinated person is exposed to the light from the street lamp? No, that’s not true: The…

Understanding DNA Analysis Deep Dive into Xcode Life Deep Dive into Promethease Direct Comparison: Xcode Life vs. Promethease User Considerations When Choosing a DNA Analysis Tool The Verdict Important disclosure: we’re proud affiliates of some tools mentioned in this guide. If you click an affiliate link and subsequently make a…

Tapper EB, Curry MP. Hepatitis Caused by Other Viruses Handbook of Liver Disease, 2018: p. 78. Sarin SK, Kumar M. Viral hepatitis A, in Molecular Pathology of Liver Diseases. Springer; 2011. pp. 527–52. Qu B, Brown RJ. Strategies to inhibit Hepatitis B Virus at the transcript level. Viruses. 2021;13(7):1327. Article  CAS …

1. Introduction The World Health Organization (WHO), on 11 March 2020, declared the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) as a pandemic [1,2]. The causative virus is a newly discovered pathogenic member of the beta coronavirus genus, which causes the coronavirus disease 2019 (COVID-19). The pathogen has primarily been…

Plasmid design The information on each plasmid for combinatorial libraries is indicated in Supplementary Table S1. Plasmids used in this study to generate combinatorial libraries for SpCas9, enAsCas12a, CHyMErA, enAsCas12a (dual-gRNA), CHyMErA.v2, multiSPAS were deposited to Addgene (IDs: 189632, 189633, 189634, 189635, 189636, 189637, respectively). 3Cs oligonucleotide design The protocol…

ABSTRACT Coronavirus disease 2019 (COVID-19) caused by the SARS-CoV-2 virus has led to a global pandemic with a high spread rate and pathogenicity. Thus, with limited testing solutions, it is imperative to develop early-stage diagnostics for rapid and accurate detection of SARS-CoV-2 to contain the rapid transmission of the ongoing…

Igbokwe, I. O. Evolving anti-disease strategies from biochemical pathogenesis of African trypanosomiasis. Adv. Cytol. Pathol. 3(2), 33–39 (2018). Google Scholar  Njiru, Z. K., Constantine, C. C., Gitonga, P. K., Thompson, R. C. & Reid, S. A. Genetic variability of Trypanosoma evansi isolates detected by inter-simple sequence repeat anchored-PCR and microsatellite….

MicroRNAs (miRNAs) are small noncoding RNAs consisting of approximately 20–25 nucleotides. As a kind of transcription regulation factors, miRNAs perform post-transcriptional repression by binding to the 3′UTR of target in the seed region, which is considered as a typical mode of miRNA-mediated gene regulation [[1], [2], [3]]. And they play…

Materials The materials used for cell cultures and characterization are listed in Additional file 1: Table S1. Cell sources After getting informed consent, PB was drawn from three healthy O, Rh D-positive donors. CB was collected from three healthy newborn babies at the Department of Obstetrics and Gynecology at Severance…

Following the first reports of Severe fever with thrombocytopenia syndrome virus (SFTSV) during 2009 in Central China [1], there have been outbreaks reported in over 20 Chinese provinces in subsequent years [2], as well as SFTSV cases reported in Japan [3] and Korea [4]. The early, non-specific clinical symptoms of…

Bisoffi Z, Buonfrate D, Montresor A, Requena-Méndez A, Muñoz J, Krolewiecki AJ, et al. Strongyloides stercoralis: a plea for action. PLoS Negl Trop Dis. 2013;7:5. Google Scholar  Schär F, Trostdorf U, Giardina F, Khieu V, Muth S, Marti H, et al. Strongyloides stercoralis: global distribution and risk factors. PLoS Negl…

1. Introduction Since 1960s, goat milk has attracted people’s attention as high-value milk [1]. Its composition is similar to human milk, and it is rich in nutrients with low allergenicity [2]. In addition, goat milk contains active factors such as superoxide dismutase and DHA [3], which are beneficial to human…

Gray IC, Campbell DA, Spurr NK. Single nucleotide polymorphisms as tools in human genetics. Hum Mol Genet. 2000;9:2403–8. Article  CAS  PubMed  Google Scholar  Shastry BS. SNP alleles in human disease and evolution. J Hum Genet. 2002;47:561–6. Article  CAS  PubMed  Google Scholar  Rocha D, Gut I, Jeffreys AJ, Kwok P-Y, Brookes…

Almazán C, González-Álvarez VH, Fernández de Mera IG, Cabezas-Cruz A, Rodríguez-Martínez R, De la Fuente J (2016) Molecular identification and characterization of Anaplasma platys and Ehrlichia canis in dogs in Mexico. Ticks Tick Borne Dis 7:276–283. doi.org/10.1016/j.ttbdis.2015.11.002 Article  PubMed  Google Scholar  Aman R, Mahas A, Marsic T, Hassan N, Mahfouz…

Manufacturer : Toyota Japan Series : Toyota Corolla X Limited. Condition : Excellent Condition. •Model : 2005. •Registration : 2008. •Mileage : 74923 km. •Engine : 1NZ- •Serial : D.M. GA-21- •Engine Capacity : 1500 cc . •Color : Beige. •Fuel System : Octane & CNG. Options : ☞Key start….

In order to ensure the prevention and control of methicillin-resistant Staphylococcus aureus (MRSA) infection, rapid and accurate detection of pathogens and their resistance phenotypes is a must. Therefore, this study aimed to develop a fast and precise nucleic acid detection platform for identifying S. aureus and MRSA. We initially constructed…

Bengaluru: From monitor lizards and pangolins to tigers and cattle, Dr Sandeep Gupta and his team have lab-tested just about every kind of meat there is, submitting more than 3,000 forensic reports over the last two decades or so. As the nodal officer at the Wildlife Institute of India’s (WII) wildlife…

In a recent study published in Micromachines, researchers assessed the efficiency of a genetic decoding system for diagnosing non-small cell lung cancer (NSCLC). Study: Intelligent Genetic Decoding System Based on Nucleic Acid Isothermal Amplification for Non-Small Cell Lung Cancer Diagnosis. Image Credit: Anucha Tiemsom/Shutterstock Background NSCLC is one of the…

If you are a data scientist, chances are you have spent countless hours staring at a screen, analyzing and manipulating data. The bright white background of many popular data science tools and platforms can cause eye strain and fatigue, which can lead to decreased productivity and even headaches. Fortunately, Kaggle,…

Introduction Maize (Zea mays L.) is one of the most important cereal crops in the world. In 2011, a large outbreak of maize lethal necrosis disease (MLND) occurred in the Southern Rift Valley of Kenya (Wangai et al., 2012), caused up to 100% losses in maize yield, and affected the income…

This protocol presents the establishment of deep hypothermic circulatory arrest in rats, which can be applied to investigate systemic inflammatory response syndrome, ischemia/reperfusion injury, oxidative stress, neuroinflammation, etc. Establishment of deep hypothermic circulatory arrest in rat. Deep hypothermic circulatory arrest, DHCA is routinely applied during surgeries of complex congenital heart…

Presented here are methods for preparing active nematics from microtubules and kinesin motors, including protein preparation and construction and the use of wells for active nematic confinement. Active Nematics have emerged as an exciting research topic that expands the fields of nonlinear dynamics and liquid crystals. There are complex fluid…

There are more than 7,000 rare diseases that affect hundreds of millions of people worldwide. As Rare Disease Day is observed on February 28, here are five recent advancements that have transformed research and boosted the scope for the development of novel therapies.  FDA approves tissue-agnostic therapies for rare cancers…

PRESS RELEASE Published February 26, 2023 “Diffuse Large B-Cell Lymphoma (DLBCL) Therapeutics Market” Report provides a Detailed analysis of global market size, regional and country-level market size, segmentation market growth, market share, competitive Landscape, sales analysis, Impact of domestic and global market players, Key players profiled in the report are…

1 Introduction Recombinant therapeutic proteins, also known as biologics, have been successfully used to treat a wide range of diseases, including cancers, inflammatory disorders, and cardiovascular diseases (Walsh, 2018). Biosimilars with comparable efficacy and safety profiles have emerged as alternative options to innovator therapies. A biosimilar is a biologic that…

. 2023 Jan-Dec;15(1):2175311. doi: 10.1080/19420862.2023.2175311. Affiliations Expand Affiliations 1 CEA, INRAE, Medicines and Healthcare Technologies Department, Université Paris-Saclay, SIMoS, France. 2 Sanofi, Large Molecule Research, Vitry-sur-Seine, France. 3 Sanofi, Integrated Drug Discovery, Vitry-sur-Seine, France. Item in Clipboard Tiphanie Pruvost et al. MAbs. 2023 Jan-Dec. Show details Display options Display options Format…

Rocket Pharmaceuticals, Inc says the U.S. Food and Drug Administration (FDA) has granted regenerative medicine advanced therapy (RMAT) designation to RP-A501. RP-A501 is the company’s investigational adeno-associated virus (AAV)-based gene therapy for the treatment of Danon disease, a devastating and fatal genetic cardiac disease for which there are no disease-altering…

  Published 1 February 2023 Closing Date 20 February 2023 Workplace Grenoble, Rhône-Alpes, France Category Position Unit Laboratory Manager Scientific Services and Support EMBL Grenoble is seeking to recruit a Unit Laboratory Manager to support our dynamic, interdisciplinary, and international community, where cutting-edge research in biochemistry, cellular, structural and molecular…

Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) complex, remains one of the leading infectious causes of death worldwide, with an estimated 1.6 million TB deaths in 2021 (World Health Organization, 2022). Rapid and accurate TB diagnosis is critical to ensure timely initiation of anti-TB therapy (Walzl et al., 2018)….

Introduction Helicobacter pylori (H. pylori) is a Gram-negative, spiral microaerophilic bacterium that infects more than half of the world’s population.1,2 Studies have shown that this bacterium is the main pathogenic factor in chronic gastritis and peptic ulcers, and is related to the occurrence and development of gastric cancer.3,4 For several…

Secondary use of biopsies as research specimens The ten melanoma samples used in this study were collected by Sanford Health and classified by a board-certified pathologist. Their secondary use as research specimens (fully de-identified formalin-fixed, paraffin-embedded (FFPE) blocks) was approved under Sanford Health IRB protocol 640 (titled ‘Understanding and improving…

Porcine circovirus 2 (PCV2) is the main pathogen of porcine circovirus-associated disease (PCVAD), which can cause considerable economic loss to the pig industry. The diagnosis of PCVAD is complicated and requires a series of clinical, pathological, and virological methods. Therefore, a rapid, highly sensitive, on-site, and visual diagnostic approach would…

There are over 150 thousand people affected by non-Hodgkin’s lymphoma in Italy. Two out of 3 Italians declare that they know lymphomas, 3% have had direct experience of them, but they have limited and superficial knowledge: only 20% recognize at least 3 symptoms. Diffuse large B-cell lymphoma (Dlbcl) is the…

As seen with the COVID-19 pandemic, detection methods that are rapid, simple, accurate, and sensitive are vital for detecting viral pathogens and for controlling the spread of infectious diseases. Unfortunately, laboratory-based methods often require trained personnel and involve complex procedures. In a new study, researchers at the University of Illinois…

  In a latest research posted to the Research Square* preprint server, researchers used isothermal amplification with clustered often interspaced brief palindromic repeats (CRISPR)-Cas12a to detect extreme acute respiratory syndrome coronavirus 2 (SARS-CoV-2).  Study: Rapid, multiplex detection of SARS-CoV-2 utilizing isothermal amplification coupled with CRISPR-Cas12a. Image Credit: creativeneko/Shutterstock Background To…

19 Pages Posted: 23 Mar 2022 See all articles by Pengfei Song University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology…

Here, we present an improved chemotaxis assay protocol. The goal of this protocol is to reduce the steps and costs of traditional bacterial chemotaxis methods and to serve as a valuable resource for understanding plant-microbe interactions. The paper reported identification of chemoattractant of rhizobacteria in root exudates using an improved…

doi: 10.3389/fbioe.2022.845688. eCollection 2022. Affiliations Expand Affiliations 1 Provincial Key Laboratory for Transfusion-Transmitted Infectious Diseases, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China. 2 Clinical Laboratory Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China. 3 Department of Emergency, The Traditional…

Introduction About 700,000 people die from “superbugs” globally every year. Antibiotic abuse is the culprit of extensive spread of superbugs. Currently, carbapenemase-producing organisms are among the most important pathogens of hospital infections. Especially the plasmid-mediated highly transmissible carbapenem-resistant Enterobacterales (CRE) has become an important public health issue of global concern….

The interaction of an ATP-dependent chromatin remodeler with a DNA ligand is described using CD spectroscopy. The induced conformational changes on a gene promoter analyzed by the peaks generated can be used to understand the mechanism of transcriptional regulation. The protocol allows us to visualize the changes in the second…

This protocol is dedicated to the microtubule plus-end visualization by EB3 protein transfection to study their dynamic properties in primary cell culture. The protocol was implemented on human primary skin fibroblasts obtained from Huntington’s disease patients. The Huntington’s disease, HD, is an incurable, neurodegenerative pathology caused by a mutation in…

1 0q26 FGFR2 Break Apart FISH Probe Kit For Research Use Only Not for Use in Diagnostic Procedures 0q26 FGFR2 Break Apart FISH Probe Kit 09N /R2 Key to Symbols Used 09N /R2 Reference Number Lot Number Global Trade Item Number Centromere D0S294 0q26. Region FGFR2 5 ATE SHGC-529 Telomere…

A scientist uses a pipette to place a sample on a GridION in an Oxford Nanopore lab. Oxford Nanopore, the pioneer developer of DNA/RNA nanopore sensing tools that include its pocket-sized MinION DNA/RNA sequencer, has disclosed plans for an initial public offering that could value the company at a reported…

Sweden’s Cellink AB has rebranded to BICO, the world’s first Bio-Convergence company. BICO is the parent company overseeing the 11 subsidiaries, including Cellink Bioprinting. This transition is expected to enable BICO and its subsidiaries to innovate through the group’s shared bio convergence agenda, which entails investments in bioengineering, technology development,…