Tag: LoF

A Guide for Optimizing Arrayed CRISPR Screens

Loss-of-function (LOF) screens measure the phenotypic effects of gene disruptions, which helps researchers identify and validate gene targets for drug discovery. CRISPR-Cas9 technology has enabled great advances in LOF screening. While researchers typically use pooled CRISPR screens to assess fitness phenotypes, arrayed screens are amenable to a variety of functional…

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Loss of gene linked to differences in brain size

The work, from the Wellcome Sanger Institute, the French Institute of Health and Medical Research (Inserm), the University of Bourgogne Franche-Comté, and Beijing Genomics Institute (BGI)-Shenzhen, found that when there was a loss-of-function mutation in the mouse gene Magee2, it led to adult male mice having slightly enlarged brains. When…

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Inquiry related to vcf file and formatting

Hello everyone, I am trying to run predixcan software. But its showing error as segmentation fault implying that there is something wrong with my vcf files. I am sharing the header of vcf file. ##fileformat=VCFv4.1 ##INFO=<ID=LDAF,Number=1,Type=Float,Description=”MLE Allele Frequency Accounting for LD”> ##INFO=<ID=AVGPOST,Number=1,Type=Float,Description=”Average posterior probability from MaCH/Thunder”> ##INFO=<ID=RSQ,Number=1,Type=Float,Description=”Genotype imputation quality from…

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