Tag: LwaCas13a

Materials and Instruments Clinical Strains and Sputum Samples Collection A total of 117 bacterial strains were isolated and collected from sputum samples of individual patients with suspected intestinal infection treated at our hospital during the first half of 2021. Carbapenemase production was confirmed by the Modified Carbapenem Inactivation Method (mCIM)…

The gold standard for rapid and specific detection of infectious disease agents is nucleic acid-based assays, such as polymerase chain reaction (PCR), but are costly, instrumentally expensive, and require highly trained personnel. Assays based on the CRISPR-Cas system have recently emerged as an alternative to PCR-based diagnostics. CRISPR detection relies…

LwaCas13a (previously known as LwaC2c2) is a crRNA-mediated RNA endonuclease derived from Leptotrichia wadeim. It can specifically recognize and cleave the RNA target with the PFS sequence. In addition, Cas13a also has the trans-cleavage activity, that is, when LwaCas13a binds crRNA and RNA target to form a ternary complex,…

Summary CRISPR systems are prokaryotic adaptive immune systems that use RNA-guided Cas nucleases to recognize and destroy foreign genetic elements. To overcome CRISPR immunity, bacteriophages have evolved diverse families of anti-CRISPR proteins (Acrs). Recently, Lin et al. (2020) described the discovery and characterization of 7 Acr families (AcrVIA1–7) that inhibit…

Scientists Used Genetic Engineering to Change How High Cocaine Got Mice In Study ABSTRACT breaks down mind-bending scientific research, future tech, new discoveries, and major breakthroughs. Scientists have shown that the effects of cocaine in mice can be enhanced by microbes that live in the digestive tract, shedding light on…

doi: 10.1038/s41589-022-01135-y. Online ahead of print. Jie Yang #  1 , Yang Song #  2   3 , Xiangyu Deng  4 , Jeffrey A Vanegas  1 , Zheng You  1 , Yuxuan Zhang  2   3 , Zhengyan Weng  2   3 , Lori Avery  5 , Kevin D Dieckhaus  6 , Advaith Peddi  4 , Yang Gao  7 , Yi Zhang  8…

Introduction The clustered regularly interspaced short palindromic repeats (CRISPR)–CRISPR-associated (Cas) protein systems naturally exist in prokaryotes. They are RNA-mediated defense mechanisms to protect bacteria and archaea from invading nucleic acids (Barrangou et al., 2007). CRISPR–Cas systems are classified into two classes (1 and 2). Class 2 CRISPR–Cas require a single…

This article was originally published here Anal Chim Acta. 2022 May 1;1205:339749. doi: 10.1016/j.aca.2022.339749. Epub 2022 Mar 22. ABSTRACT The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design…