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Biosensors | Free Full-Text | CRISPR/Cas12a-Based Detection Platform for Early and Rapid Diagnosis of Scrub Typhus
1. Introduction Orientia tsutsugamushi (OT) is an obligate intracellular parasite bacteria and the causative agent of scrub typhus (ST), which is associated with acute febrile illness (AFI) [1] and transmitted by mites through an infected chigger bite (in the larval stage). This disease, which was earlier believed to be endemic…
Inhibition of providencia vermicola FabD
Introduction Microbial resistance has become a problem in the contemporary medical environment due to the inadequate development of new antibiotics and the asinine prescription of antibiotics in both developed and developing nations. Antimicrobial resistance (AMR), which affects wealthy and developing nations, is a global concern.1 AMR is a problem for…
Genome-wide analysis and characterization of the LRR-RLK gene family provides insights into anthracnose resistance in common bean
Identification of PvLRR-RLK genes From the kinome of P. vulgaris30, 1203 PKs were identified. Of these, only the proteins endowed with the transmembrane kinase and LRR domains were retained (Supplementary Table S1). All PvLRR-RLKs obtained were analyzed for redundancy following the criterion of maintaining the largest variants in the case…
Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a | AMB Express
Reagents Primers for RPA were synthesized by TsingKe Biological Technology (Beijing, China). Recombinant RNase inhibitor, crRNA, and RNase-free water were obtained from Takara Bio (Beijing, China). The ssDNA reporter was synthesized by Sangon Biotech (Shanghai, China). The TIANamp Bacteria DNA kit was purchased from TIANGEN (Beijing, China). The TwistAmp Basic…
Death of a zoo hippopotamus underscores the species’ susceptibility to SARS-CoV-2
A recent Emerging Infectious Diseases study investigated the death of a hippopotamus at a zoo in Hanoi, Vietnam. Interestingly, this study detected the presence of the severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus in the hippopotamus. SARS-CoV-2 is the causal agent of the ongoing coronavirus disease 2019 (COVID-19) pandemic. Study:…
Characterization and Structural Prediction of ORF10, ORF7b, ORF7a, ORF6, Membrane Glycoprotein, and Envelope Protein in SARS-CoV-2 Bangladeshi Variant through Bioinformatics Approach
Abstract The acute respiratory disease induced by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global epidemic in just less than a year by the first half of 2020. The subsequent efficient human-to-human transmission of this virus eventually affected millions of people worldwide. The most devastating thing…
A problem with MEGA-X tool for select the columns of nucleotides and edit them
A problem with MEGA-X tool for select the columns of nucleotides and edit them 2 Hello I have a problem with MEGA-X tool when I try to edit the columns of nucleotides. In the last version of this tool the user can aligned the nucleotides and then selected some columns…