Tag: mESCs
Report Exposes Rot In Middle East Studies Centers, Connection to CRT
Neetu Arnold, Senior Research Associate of the National Association of Scholars (NAS) has done what amounts to an expose of the politicization of campus Middle East studies centers. What began as – let’s say, a coincidence of timing between generous Arab donations to university study centers offering a pro-Arab, anti-Israel…
Absolute Scaling of Single-Cell Transcriptomes Reveals Pervasive Hypertranscription in Adult Stem and Progenitor Cells
Introduction Single cell RNA-seq (scRNA-seq) is a powerful tool to measure gene expression in individual cells. While relative gene expression differences are currently analyzed with great interest in single cell data, differences in total transcript levels were mainly considered technical artefacts and removed during normalization, assuming that single cells contain…
Frontiers | Hair Graying Regulators Beyond Hair Follicle
Introduction Hair graying is one of the representative signs of aging. It has been considered to be triggered by a decreased number of follicular melanocyte stem cells (MeSCs; Nishimura et al., 2005; Iida et al., 2020) or dysfunction of MeSCs such as decreased oxidation resistance capacity with aging (Shi et…
From mESCs to mEPSCs: A proteomics-based study
Out laboratory has derived mEPSCs from mESCs, which represents a breakthrough in stem cell research. Unlike the standard pluripotent stem cells, mEPSCs can contribute to both embryonic and extra-embryonic lineages in the chimera assay (Yang et al., 2017a). In the development of EPSC culture conditions, many small molecules are tested…
Figure 4 | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation
p57 suppressed the proliferation of mESCs. (a) The growth curve of control () and p57 knockdown (shp57, ) mESCs. (b, c) EdU staining results of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (d) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of…
p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation
Embryonic stem cells (ESCs) are pluripotent stem cells that have indefinite self-renewal capacities under appropriate culture conditions in vitro. The pluripotency maintenance and proliferation of these cells are delicately governed by the concert effect of a complex transcriptional regulatory network. Herein, we discovered that p57Kip2 (p57), a cyclin-dependent kinase inhibitor…
HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCs
doi: 10.1093/nar/gkab1221. Online ahead of print. Yang Yang 1 2 , Liwei Zhang 3 , Chaoyang Xiong 2 , Jun Chen 2 4 5 , Li Wang 6 , Zengqi Wen 2 5 , Juan Yu 2 , Ping Chen 4 , Yanhui Xu 6 , Jingji Jin 1 7 8 , Yong Cai …
