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Tag: mESCs
Results obtained by analyzing experiment data on mESCs in response to the varying E-P genomic distance.
(A) Distributions of the mRNA numbers of smRNA-FISH in different cell lines. The solid line shows the best fit of our stochastic multiscale model to the experimental data shown in the histograms. dG = NaN represents promoter-only control cell line. Parameter values are listed in Table E in S1 Text….
Mouse Embryonic Stem Cells | ATCC
Mouse embryonic stem cells (mESCs) were first isolated and propagated in culture in 1981. mESCs are typically isolated from blastocysts from the inner cell mass of 3.5-day-old embryos and have the potential to generate every cell type found in the body. They can be maintained in an undifferentiated, pluripotent state…
Scientists create ‘cloaked’ donor cell and tissue grafts that escape rejection by the immune system
a, Candidate immunomodulatory factors and their roles in the innate and adaptive immune pathways involved in rejection of non-self. The underlined factors were selected to generate transgene-containing vectors. b, Schema showing the generation of clonal B6 mESCs that express the selected eight immunomodulatory transgenes via insertion with piggyBac (PB) and…
AHCY plays multiple roles in maintaining the identity and pluripotency of mouse embryonic stem cells
Adenosylhomocysteinase (AHCY), a key enzyme in the methionine cycle, is essential for the development of embryos and the maintenance of mESCs. However, the precise underlying mechanism of Ahcy in regulating pluripotency remains unclear. As the only enzyme that can hydrolyze S-adenosylhomocysteine in mammals, AHCY plays a critical role in the…
Immune-privileged tissues formed from immunologically cloaked mouse embryonic stem cells survive long term in allogeneic hosts
Mice C57BL/6N (strain 005304), C3H/HeJ (strain 000659), FVB/NJ (strain 001800), BALB/cJ (strain 000651) and NSG mice (stock 005557) were purchased from the Jackson Laboratory. CD-1 (stock 022) mice were purchased from Charles River. Mice (6–20-week-old) of each strain/background were used for teratoma assays. Mice were housed in a pathogen-free facility…
Optimized protocol to prevent disease transmission through maternal mitochondrial DNA carryover
Several human diseases arise due to defects in mitochondrial DNA (mtDNA), which is distinct from nuclear DNA and is inherited only from maternal ancestors. The prevention of these diseases by mitochondrial replacement (MR) therapy involves the risk of increasing mtDNA mutations, which can subsequently lead to mitochondrial genetic drift. A…
High-quality single-cell transcriptomics from ovarian histological sections during folliculogenesis
Introduction Single-cell RNA sequencing (RNA-seq) was first achieved by using a quantitative cDNA amplification method and applied to mouse oocytes (Kurimoto et al, 2006; Tang et al, 2009). It has since provided unprecedented opportunities for the study of cellular differentiations, states, and diseases in various biological fields, including developmental biology,…
Loss of Dip2b leads to abnormal neural differentiation from mESCs
Background: Disco-interacting protein 2 homolog B is a member of the Dip2 family encoded by the Dip2b gene. Dip2b is widely expressed in neuro-related tissues and is essential in axonal outgrowth during embryogenesis. Methods: Dip2b knockout mouse embryonic stem cell line was established by CRISPR/Cas9 gene-editing technology. The commercial kits…
Rhox6 regulates the expression of distinct target genes to mediate mouse PGCLC formation and ESC self-renewal | Cell & Bioscience
Overexpression of Rhox6 promotes PGCLC formation To discover potential candidate genes that may be important for the specification of mouse PGCs, we analyzed the transcriptional data of E9.5 PGCs and epiblasts and found that the homeobox family members Rhox6 and Rhox9, as well as the PGC markers Prdm14, Blimp1, Stella…
Successful generation of functional parathyro
image: A. Overview of the parathyroid gland production method in this study. B. Fluorescent images of mouse ESC-derived PTGs generated in PTG-deficient mouse by single-step blastocyst complementation. Left: tdTomato, middle: antibody staining for Parathyroid hormone, right: merged image. C. Transplantation experiment of mESC-derived PTGs into mice modeling post-surgical hypoparathyroidism. The plasma…
Crosstalk between RNA m6A and DNA methylation regulates transposable element chromatin activation and cell fate in human pluripotent stem cells
Bourque, G. et al. Ten things you should know about transposable elements. Genome Biol. 19, 199 (2018). Article CAS PubMed PubMed Central Google Scholar Chuong, E. B., Elde, N. C. & Feschotte, C. Regulatory activities of transposable elements: from conflicts to benefits. Nat. Rev. Genet. 18, 71–86 (2017). Article CAS …
L-type Ca2+ channels and SK channels in mouse embryonic stem cells and their contribution to cell proliferation
dc.contributor.author Araujo, Raquel dc.contributor.author Martín, Franz dc.contributor.author Soria, Bernat dc.contributor.author Vegara Meseguer, Josefina María dc.contributor.author Pérez Sánchez, Horacio dc.date.accessioned 2018-05-10T11:35:45Z dc.date.available 2018-05-10T11:35:45Z dc.date.issued 2015-01-23 dc.identifier.other DOI 10.1007/s00232-015-9779-8 dc.identifier.uri hdl.handle.net/10952/3118 dc.description.abstract Mouse embryonic stem cells (mESCs) are capable of both self-renewal and multilineage differentiation; thus, they can be expanded in vivo…
The roles and implications of RNA m6A modification in cancer
Huang, H., Weng, H. & Chen, J. m6A modification in coding and non-coding RNAs: roles and therapeutic implications in cancer. Cancer Cell 37, 270–288 (2020). Article CAS PubMed PubMed Central Google Scholar Qing, Y., Su, R. & Chen, J. RNA modifications in hematopoietic malignancies: a new research frontier. Blood 138,…
A multi-omics integrative analysis based on CRISPR screens re-defines the pluripotency regulatory network in ESCs
Genome-scale CRISPR screen to identify regulators that maintain mESC pluripotency To establish a function-based PGRN, we first performed a CRISPR-Cas9 mediated genome-wide screen to detect genes essential for self-renewal. mESCs were cultured under Leukaemia inhibitory factors (LIF)/serum condition (L/S), which was commonly used in similar tasks and confer a naïve…
Acetylation of histone H2B marks active enhancers and predicts CBP/p300 target genes
Multiple H2BNTac sites occupy the same genomic regions H2BNTac sites are similarly regulated by CBP/p30018 (Supplementary Fig. 1a), yet the reported genome occupancy patterns of H2BNTac sites are dissimilar from each other22,23 (Supplementary Note 1). To resolve this conundrum, we systematically compared H3K27ac and H2BNTac genomic occupancy and regulation by…
Base editing of SMN2 gene restores production of SMN protein, curing spinal muscular atrophy in mice
(A) Immunofluorescence images of spinal cord sections from wild-type Δ7SMA mice at 25 weeks that received AAV9-ABE + AAV9-GFP in a 10:1 ratio by neonatal ICV injection, stained for GFP to indicate AAV transduction, NeuN as a marker of post-mitotic neurons, and DAPI to stain all nuclei. (B) In vivo…
How to demultiplex single-end dual indexed run
Hi all, I have data from a single-end run with a dual index structure generated by the NextSeq 500 instrument. I want to do the demultiplexing by bcl2fastq tool. How should my SampleSheet.csv structure and bcl2fastq script look like? I used aSampleSheet structure (shared below) with a bcl2fastq command (mentioned…
How to demultiplex single end – dual index run
Hi all, I have data from a single-end run with a dual index structure generated by the NextSeq 500 instrument. I want to do the demultiplexing by bcl2fastq tool. How should my SampleSheet.csv structure and bcl2fastq script look like? I used aSampleSheet structure (shared below) with a bcl2fastq command (mentioned…
Full-length human dystrophin on human artificial chromosome compensates for mouse dystrophin deficiency in a Duchenne muscular dystrophy mouse model
Ethics declarations This study was approved by the Animal Care and Use Committee of Tottori University (Permit Number: 16-Y-20, 17-Y-28, 19-Y-22, 20-Y-31, 21-Y-26, and 22-Y-36). All experiments were carried out in compliance with the ARRIVE guidelines. All methods were performed in accordance with the relevant guidelines and regulations. Mice were…
The down-regulated Ctnnb1 is critical for the differentiation defects in mESCs with monoallelic R595H mutation in Trim71.
(A) Representative western blotting and quantification in the WT and the R595H/+ mESCs cultured in the 2i + Lif medium. (B) Measuring the Wnt signaling in the WT and the R595H/+ mESCs cultured in the 15% FBS + Lif medium using the luciferase assay. (C) Representative western blotting and quantification…
Genome-wide 5-hydroxymethylcytosine (5hmC) reassigned in Pten-depleted mESCs along neural differentiation
DNA methylation and hydroxymethylation have been implicated in the regulatory dynamics of gene expression in normal development and differentiation. 5-Hydroxymethylcytosine (5hmC), created by the ten-eleven translocation (TET) protein-catalyzed oxidation of 5-methylcytosine (5mC), is abundant in the brain, but the genome-wide distribution and impact of 5hmC during diverse neuronal differentiation remain…
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Report Exposes Rot In Middle East Studies Centers, Connection to CRT
Neetu Arnold, Senior Research Associate of the National Association of Scholars (NAS) has done what amounts to an expose of the politicization of campus Middle East studies centers. What began as – let’s say, a coincidence of timing between generous Arab donations to university study centers offering a pro-Arab, anti-Israel…
Absolute Scaling of Single-Cell Transcriptomes Reveals Pervasive Hypertranscription in Adult Stem and Progenitor Cells
Introduction Single cell RNA-seq (scRNA-seq) is a powerful tool to measure gene expression in individual cells. While relative gene expression differences are currently analyzed with great interest in single cell data, differences in total transcript levels were mainly considered technical artefacts and removed during normalization, assuming that single cells contain…
Frontiers | Hair Graying Regulators Beyond Hair Follicle
Introduction Hair graying is one of the representative signs of aging. It has been considered to be triggered by a decreased number of follicular melanocyte stem cells (MeSCs; Nishimura et al., 2005; Iida et al., 2020) or dysfunction of MeSCs such as decreased oxidation resistance capacity with aging (Shi et…
From mESCs to mEPSCs: A proteomics-based study
Out laboratory has derived mEPSCs from mESCs, which represents a breakthrough in stem cell research. Unlike the standard pluripotent stem cells, mEPSCs can contribute to both embryonic and extra-embryonic lineages in the chimera assay (Yang et al., 2017a). In the development of EPSC culture conditions, many small molecules are tested…
Figure 4 | p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation
p57 suppressed the proliferation of mESCs. (a) The growth curve of control () and p57 knockdown (shp57, ) mESCs. (b, c) EdU staining results of control () and p57 knockdown (shp57, ) mESCs cultured for 48 h. (d) Real-time PCR analyses of pcna, Cyclin A, and Cyclin E expression levels of…
p57 Suppresses the Pluripotency and Proliferation of Mouse Embryonic Stem Cells by Positively Regulating p53 Activation
Embryonic stem cells (ESCs) are pluripotent stem cells that have indefinite self-renewal capacities under appropriate culture conditions in vitro. The pluripotency maintenance and proliferation of these cells are delicately governed by the concert effect of a complex transcriptional regulatory network. Herein, we discovered that p57Kip2 (p57), a cyclin-dependent kinase inhibitor…
HIRA complex presets transcriptional potential through coordinating depositions of the histone variants H3.3 and H2A.Z on the poised genes in mESCs
doi: 10.1093/nar/gkab1221. Online ahead of print. Yang Yang 1 2 , Liwei Zhang 3 , Chaoyang Xiong 2 , Jun Chen 2 4 5 , Li Wang 6 , Zengqi Wen 2 5 , Juan Yu 2 , Ping Chen 4 , Yanhui Xu 6 , Jingji Jin 1 7 8 , Yong Cai …