Tag: MiRBase

What does the part “mature miRBase miRNAs not detected by miRDeep2” actually mean in a miRDeep2 analysis? Should those hits be included in results?

What does the part “mature miRBase miRNAs not detected by miRDeep2” actually mean in a miRDeep2 analysis? Should those hits be included in results? 0 After completion of a miRDeep2 analysis, the results are generated in three different parts, viz., 1) novel miRNAs predicted by miRDeep2, 2) mature miRBase miRNAs…

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Mapped reference id is not an id of the genome file genome_nowhitespace.fa

miRDeep2: Mapped reference id is not an id of the genome file genome_nowhitespace.fa 1 Hi everyone, I’m trying to run nf-co.re/smrnaseq pipeline and I’m having a problem with mirdeep2. Command: nextflow run nf-core/smrnaseq -profile ijcluster –input /home/794_both.fastq.gz –outdir /home/results –genome GRCh38 –protocol qiaseq –mature mirbase.org/ftp/CURRENT/mature.fa.gz –hairpin mirbase.org/ftp/CURRENT/hairpin.fa.gz Error message: Command…

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assign in pandas pipeline – DevDreamz

You can use pipe: tmp_df = df.\ drop(“Gene type”, axis=1).\ rename(columns = { “Gene stable ID”: “ENSG”, “Gene name”: “gene_name”, “miRBase accession”: “MI”, “miRBase ID”: “mirna_name” }).\ pipe(lambda x: x.assign(species = x.mirna_name.str[:3])) tmp_df Out[365]: ENSG gene_name MI mirna_name species 0 ENSG00000274494 MIR6832 MI0022677 hsa-mir-6832 hsa 1 ENSG00000283386 MIR4659B MI0017291 hsa-mir-4659b…

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Htseq is giving me 0 counts using the GFF3 of miRBase

Hello! I am trying to annotate a miRNA-seq so that it gives me mature miRNAs where I already have 5p and 3p. For this, I have used the index mm10.fa and the miRBase mmu.gff3. I have aligned with HISAT2 and am trying to count with HTSeq, however I get 0…

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Extracellular circulating miRNAs as stress-related signature to search and rescue dogs

Study approval was provided by the Research Ethics Committee of the University of Perugia (report n.2018-21 of 11/12/2018) according to Italian Ministry of Health legislation18. All methods were carried out following relevant guidelines and regulations and the study was carried out in compliance with the ARRIVE guidelines. Informed consent is…

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UMItools dedup deduplication taking too much time + RAM

I have some RNAseq data from miRNAs that I have processed with Bowtie2 (aligning to miRBase). Now, when doing the deduplication with umi_tools dedup I find that some of the files take a lot of time+RAM to finish (some files take around 3-4 minutes and 4-5GB of RAM and some…

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mirbase ftp error

mirbase ftp error 0 When I am running this link “wget mirbase.org/pub/mirbase/21/hairpin.fa.gz” in the terminal the below error is appearing for me. How can I fix it? -2022-01-21 16:35:30– mirbase.org/pub/mirbase/21/hairpin.fa.gz => ‘hairpin.fa.gz’ Resolving mirbase.org (mirbase.org)… 130.88.97.249 Connecting to mirbase.org (mirbase.org)|130.88.97.249|:21… failed: Connection refused. ftp mirbase • 225 views • link…

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Bioconductor – mirbase.db

DOI: 10.18129/B9.bioc.mirbase.db     This package is for version 3.11 of Bioconductor; for the stable, up-to-date release version, see mirbase.db. miRBase: the microRNA database Bioconductor version: 3.11 miRBase: the microRNA database assembled using data from miRBase (www.mirbase.org/). Author: James F. Reid <reidjf at gmail.com> Maintainer: James F. Reid <reidjf at…

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assign in pandas pipeline – Stackify

You can use pipe: tmp_df = df. drop(“Gene type”, axis=1). rename(columns = { “Gene stable ID”: “ENSG”, “Gene name”: “gene_name”, “miRBase accession”: “MI”, “miRBase ID”: “mirna_name” }). pipe(lambda x: x.assign(species = x.mirna_name.str[:3])) tmp_df Out[365]: ENSG gene_name MI mirna_name species 0 ENSG00000274494 MIR6832 MI0022677 hsa-mir-6832 hsa 1 ENSG00000283386 MIR4659B MI0017291 hsa-mir-4659b…

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Omics advanced 07 | transcriptome mRNA | miRNA | lncrna

A brief introduction mRNA,miRNA,lncRNA And circRNA Principle of genome sequencing 、 Routine analysis of Shengxin . Catalog Introduction to transcriptome sequencing mRNA|miRNA|lncRNA|circRNA Introduction to analysis mRNA Group miRNA Group lncRNA Group circRNA Group mRNA|miRNA|lncRNA|circRNA Correlation analysis Reference Introduction to transcriptome sequencing Transcriptome (Transcriptome) Is a single or group of cells…

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Biostar Systems

Comment: STAR vs Novoalign IGV Browser visualization by chasem &utrif; 10 That is good to know that it isn’t just my set of reads…still concerning, though. Comment: STAR vs Novoalign IGV Browser visualization by chasem &utrif; 10 I was not expecting this — not sure what to make of it…

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MiRBase miRNA analysis with STAR

MiRBase miRNA analysis with STAR 0 Hi All, I am using the latest mice reference genome (GRCm39) for small RNAseq/miRNA-seq analysis. MiRBase database doesn’t have any GFF/GTF file for the mouse mature-miRNA/loop-miRNA. I just have mature-miRNA and loop-miRNA fasta sequences from MiRBase. How I can use the STAR tool to…

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Inquiry related to vcf file and formatting

Hello everyone, I am trying to run predixcan software. But its showing error as segmentation fault implying that there is something wrong with my vcf files. I am sharing the header of vcf file. ##fileformat=VCFv4.1 ##INFO=<ID=LDAF,Number=1,Type=Float,Description=”MLE Allele Frequency Accounting for LD”> ##INFO=<ID=AVGPOST,Number=1,Type=Float,Description=”Average posterior probability from MaCH/Thunder”> ##INFO=<ID=RSQ,Number=1,Type=Float,Description=”Genotype imputation quality from…

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microarray miRNA expression data analysis

I wrote a script on how to analyze the microarray-based miRNA expression data. Here is my code: # general config baseDir <- ‘.’ annotfile <- ‘mirbase_genelist.tsv’ setwd(baseDir) options(scipen = 99) require(limma) # read in the data targets <- read.csv(“/media/mdrcubuntu/46B85615B8560439/microarray_text_files/targets.txt”, sep=””) # retain information about background via gIsWellAboveBG project <- read.maimages(targets,source=”agilent.median”,green.only…

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