Tag: mRNA

Get mRNA sequence from amino acid sequence using BioPython

Get mRNA sequence from amino acid sequence using BioPython 1 I have a sequence of amino acids like “MVLLV” and I want to know what mRNA corresponds to that, I’m using biopython for this, but I just know the back_transcribe(), to revert the RNA to DNA, how can I do…

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Moderna’s mRNA-1647 Vaccine Shows Promise in Phase 3 Clinical Trials for CMV

Exciting progress is being made in the world of vaccine development as Moderna, Inc., the renowned American pharmaceutical and biotechnology company, has reported highly promising results for their experimental mRNA vaccine against cytomegalovirus (CMV). The vaccine, known as mRNA-1647, is the first of its kind to enter phase 3 clinical…

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KEGG T01015: 4329963

Entry 4332822           CDS       T01015                                  Name (RefSeq) LOW QUALITY PROTEIN: nuclear cap-binding protein subunit 1-like   KO K12882   nuclear cap-binding protein subunit 1 Organism osa  Oryza sativa japonica (Japanese rice) (RefSeq) Pathway osa03013   Nucleocytoplasmic transport osa03015   mRNA surveillance pathway osa03040   Spliceosome Brite KEGG Orthology (KO) [BR:osa00001] 09120 Genetic Information Processing  09121 Transcription   03040 Spliceosome    4332822  09122 Translation   03013 Nucleocytoplasmic transport    4332822   03015 mRNA…

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Treating the Untreatable with Precision Therapies – A $4 trillion Opportunity?

Precision Therapies For Targeted Results Precision therapies have always been the ultimate goal of medicine. Instead of drugs acting on multiple parts of the body, these therapies would only target one organ, one cell, or even one gene. In theory, not only would this be a lot more efficient, but…

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Bhubaneswar ILS scientists discover direct interaction of circular RNAs with mRNAs in gene expression

In an extremely proud moment for Odisha, a team of researchers at the prestigious Institute of Life Sciences (ILS) in Bhubaneswar has made a groundbreaking discovery that suggests the role of direct interaction of circRNAs with mRNAs in gene expression regulation. The findings represent a novel method for illuminating the…

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Did COVID vaccines kill 14X more people than they saved?

Did a study just show that COVID-19 vaccines killed 14x more people than they saved?! Take a look at the viral claim, and find out what the facts really are!   Claim : COVID Vaccines Kill 14X More People Than They Saved! People are sharing an article (archive) by The…

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what will therapy look like in 2034?

Drugs like PARP inhibitors and chimeric antigen receptor (CAR) therapies, and advancements in artificial intelligence in drug discovery, have spurred hope for improved cancer care in the past year. Rigorous research has paved the way for a declining cancer death rate – by 33% to be precise – since the…

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16 UK biotech companies you should know about

The UK is home to several biotech hubs, including the famous ‘golden triangle’, made up of Oxford, Cambridge, and London. In fact, after a $700 million surge in funding in the third quarter of 2023, the UK biotech scene is now one of the most vibrant in Europe, laying the…

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Unravelling cell type-specific responses to Parkinson’s Disease at single cell resolution | Molecular Neurodegeneration

Single nucleus RNA-seq reveals cell type heterogeneity in human SNpc We sampled SNpc from post-mortem human brains of 15 sporadic Parkinson’s disease (PD) patients and 14 Control individuals (see Supplementary Table 1 for full pathology reports). Using a 10X Genomics Chromium platform, we performed single nucleus RNA-seq (snRNA-seq) on more than…

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New RNA interference therapy shows promise in reducing high blood pressure

About 1.3 billion adults worldwide have clinically high blood pressure, many of whom may , [+] Unaware that they are living in this situation. getty Approximately 1.3 billion adults worldwide have clinically high blood pressure, many of whom may be unaware that they are living with the condition. As a…

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What is shrna transfection? | 4 Answers from Research papers

What are the applications of gene transfer in the treatment of various diseases?5 answersGene transfer has various applications in the treatment of different diseases. It can be used for gene therapy, where new genes are inserted into cancer cells to slow down their growth or induce cell death. Gene transfer…

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Ch 13 Learning Objectives (docx)

Ch 13 Learning Objectives Section 13.1 1. Differentiate between the structures of DNA & RNA (Table 13.1, Fig 13.1) 2. Know the locations and functions of rRNA, mRNA, and tRNA (Table 13.2)  For now, you only need to know rRNA, mRNA, tRNA. We will learn the others in future…

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Proteomic analysis of SARS-CoV-2 particles unveils a key role of G3BP proteins in viral assembly

Capturing proteins associated with SARS-CoV-2 virions To identify host cell proteins potentially present in extracellular SARS-CoV-2 virions, viral particles were produced from two lung epithelial cell models, A549 cells overexpressing ACE2 receptor (A549-ACE2) and Calu-3 cells that naturally express ACE2 receptor and TMPRSS2. Following infection with the D614G SARS-CoV-2 strain32,…

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Causality-enriched epigenetic age uncouples damage and adaptation

Gladyshev, V. N. et al. Molecular damage in aging. Nat. Aging 1, 1096–1106 (2021). Article  PubMed  PubMed Central  Google Scholar  Sziráki, A., Tyshkovskiy, A. & Gladyshev, V. N. Global remodeling of the mouse DNA methylome during aging and in response to calorie restriction. Aging Cell 17, e12738 (2018). Article  PubMed …

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could it be a cure?

When CASGEVY, the first CRISPR-based therapy, was approved by the FDA in December, it gave CRISPR technology the validation it needed. Any promise it had held before that moment was simply investigational. In this case, the approval of CASGEVY was granted for the treatment of two blood disorders – sickle…

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The immense challenge of cancer vaccines: ‘There are promising results’ | Science

The dream of a cancer vaccine — capable of stimulating the immune system to fight tumors — is on the horizon of the scientific community. It probably has never been as close as it is now. After years of few successes in this field, the consolidation of other immunotherapies and…

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Amid second-largest wave of COVID infections in the US, Florida’s surgeon general calls to halt mRNA vaccines

On Friday, wastewater data from Biobot Analytics and the US Centers for Disease Control and Prevention (CDC) affirmed that the US has now reached its second-highest level of COVID-19 infections of the pandemic. It is anticipated that by midweek, rates of infections will reach 2 million per day with approximately…

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CRISPR Therapeutics Highlights Strategic Priorities and

ZUG, Switzerland and BOSTON, Jan. 08, 2024 (GLOBE NEWSWIRE) — CRISPR Therapeutics (Nasdaq: CRSP), a biopharmaceutical company focused on creating transformative gene-based medicines for serious diseases, today highlighted its strategic priorities and 2024 outlook as the Company enters its next phase of growth. “We had a landmark year in 2023, marked…

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German Bundestag – No undesirable effects from DNA fragments

39 Berlin: (hib/PK) According to the federal government, there is no scientific evidence that the DNA fragments contained in anti-corona vaccines could cause undesirable effects. Every active ingredient batch of the Covid-19 mRNA vaccine is mandatory tested by the manufacturer for residual DNA amounts. The results are part of the…

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TCW’s Top Twenty: Why I don’t believe there ever was a Covid virus

As we approach the end of the year, we are repeating our twenty most-read articles of 2023, in reverse order. This is number 2 and it was first published on March 22. I’VE grown increasingly frustrated about the way debate is controlled around the topic of origins of the alleged…

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Knockout of lipoprotein lipase with CRISPR/Cas9 causes severe developmental defects and affects lipid deposition in Japanese medaka (Oryzias latipes)

Abstract In mammals, lipoprotein lipase (LPL) has been found to play an important role in lipid mentalism and deposition. LPL deficiency in humans (Homo sapiens) and mice (Mus musculus) tends to cause hypertriglyceridemia. The lpl gene is not expressed in adult mammalian liver, but is in adult fish liver. The…

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Is Australia Opportunistically Vaccinating People Under Sedation?!

Is Australia opportunistically vaccinating people under sedation?! Take a look at the viral claims, and find out what the facts really are!   Claim : Australia Is Opportunistically Vaccinating People Under Sedation! People are sharing an ATAGI advisory, while claiming or suggesting that Australia is opportunistically vaccinating people under sedation!…

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Pharmaceutics | Free Full-Text | Natural Biopolymer-Based Delivery of CRISPR/Cas9 for Cancer Treatment

Cancer is one of the leading causes of disease-associated mortality, with a rising incidence worldwide [1]. Although many therapeutic methods have been used in cancer treatment, such as chemotherapy, radiotherapy, surgery, and targeted therapy, the overall therapeutic outcome remains unsatisfactory. Therefore, developing new therapeutic means is urgently needed. With advances…

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Innovent, SanegeneBio to Co-Develop Hypertension Drug Candidate

Credit: Linda Bartlett/National Cancer Institute, via Wikimedia Innovent Biologics and SanegeneBio have entered into a collaboration and co-development agreement for SGB-3908, an siRNA drug candidate targeting angiotensinogen (AGT) for the treatment of hypertension. Under the terms of the deal, Innovent will gain an exclusive option to license future development, manufacturing,…

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Vaccines | Free Full-Text | Immunogenic Effects and Clinical Applications of Electroporation-Based Treatments

Immunotherapy can now be regarded as an attractive approach for cancer and infectious disease treatments. A variety of novel and varied therapeutic approaches are being discovered through immunology research. Among them, some treatment strategies are based on electrotransfer, also called electroporation (EP). EP is an efficient and safe method that…

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The race for RSV vaccine development and approval in 2023

It has been a bumpy ride for biopharma to develop a vaccine for the potentially deadly respiratory syncytial virus (RSV), since the 1960s. As GSK and Pfizer battled it out earlier this year, with the former’s Arexvy vaccine crowned the first-ever RSV vaccine to hit the market, a mere few…

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Staphylococcus aureus sepsis and mitochondrial accrual of the 8-oxoguanine DNA glycosylase DNA repair enzyme in mice.

Staphylococcus aureus sepsis and mitochondrial accrual of the 8-oxoguanine DNA glycosylase DNA repair enzyme in mice. Publication ,  Journal Article Bartz, RR; Suliman, HB; Fu, P; Welty-Wolf, K; Carraway, MS; MacGarvey, NC; Withers, CM; Sweeney, TE; Piantadosi, CA Published in: Am J Respir Crit Care Med RATIONALE: Damage to mitochondrial DNA…

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GPATCH4 regulates rRNA and snRNA 2′-O-methylation in both DHX15-dependent and DHX15-independent manners

doi: 10.1093/nar/gkad1202. Online ahead of print. Nidhi Kanwal  1 , Nicolai Krogh  2 , Indira Memet  1 , Nicolas Lemus-Diaz  1 , Chairini C Thomé  1 , Luisa M Welp  3   4 , Athanasia Mizi  5 , Philipp Hackert  1 , Argyris Papantonis  5 , Henning Urlaub  3   4   6   7 , Henrik Nielsen  2 , Katherine…

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csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axis | Parasites & Vectors

Human tissue samples The preoperative diagnosis of CS mainly relies on stool microscopy, enzyme-linked immunosorbent assay, or endoscopy. Fresh liver and intrahepatic bile duct specimens were collected from ICC patients with (CS-ICC group) and without (NC-ICC group) CS infection during partial hepatectomy at the First Hospital of Jilin University. Tumor tissues…

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Exploring the Latest Advances in Transcriptomics

Since the idea of genetic regulation was first postulated back in the 1950s, our scientific understanding of the transcriptome has deepened greatly.1 Looking into the transcriptome of cells and tissues has helped scientists to understand the biological processes that drive both health and disease; however, the complex and occasionally mysterious…

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The top biotech breakthroughs that defined 2023

As we look back at 2023 it is clear that the year has been marked by steady progress and notable breackthroughts in the biotech industry. From advancements in Alzheimer’s disease treatments to new approaches in cancer therapy, artificial intelligence (AI) progression, and obesity management, this year has seen important steps…

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Researchers look for ways to lower neuroinflammation

Share on PinterestResearchers are looking for more effective ways to deliver a potential therapy against neuroinflammation to the brain. Image credit: Maskot/Getty Images. Small interfering RNAs can silence the machinery that translates certain mRNAs, meaning they can also be used to stop the production of some proteins. Researchers have looked…

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Apoptosis-resistant megakaryocytes produce large and hyperreactive platelets in response to radiation injury | Military Medical Research

Animals C57BL/6-Tg (Pf4-icre) Q3Rsko/J (Pf4-Cre) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). B6;129-Tmem173tm1(flox)Smoc (Stingfl) mice were purchased from Shanghai Model Organisms Center, Inc. (Shanghai, China). C57BL/6J-Ifnar1em1(flox)Cya (Ifnar1fl) mice were purchased from Cyagen Biosciences (Guangzhou, China). Stingcko and Ifnar1cko mice were generated by crossing Pf4-Cre mice with…

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Summary of Genomics and Transcriptomics – DNA sequencing I. Goal II. Methodology A. Sanger DNA

DNA sequencing I. Goal II. Methodology A. Sanger DNA sequencing ● Up to 900 base pairs ● Primer extension with labeled ddNTPs (radioactive or fluorescent) ● Prior knowledge on target needed ● Difficult to detect variation in mixtures ● High accuracy + still in use for genotyping When it is…

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Progress of circRNA/lncRNA-miRNA-mRNA axis in atrial fibrillation [PeerJ]

Introduction The incidence and mortality rates of AF are continuously increasing, leading to serious complications such as heart failure and stroke (Fig. 1) (Sagris et al., 2021). The occurrence and development of AF involve different mechanisms and interactions (Kornej et al., 2020). AF often progresses from paroxysmal to persistent (Nattel…

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LncRNA/circRNA-mRNA networks in CARAS | JIR

Introduction Combined allergic rhinitis and asthma syndrome (CARAS), a new terminology introduced by the World Allergy Organization (WAO) in 2004, is an allergic reaction that occurs in the respiratory tract, including upper respiratory tract allergy (allergic rhinitis, AR) and lower respiratory tract allergy (asthma, AS).1,2 The incidence of AS in…

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Biotechnology Breakthroughs: How Genetic Engineering is Revolutionizing Healthcare

Share Tweet Share Share Email Among the various branches of biotechnology, genetic engineering stands out as a revolutionary field that holds immense potential for transforming healthcare as we know it. This article explores the latest biotechnological breakthroughs, focusing on how genetic engineering is…

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Big problems, small solutions: how synthetic biology is building a stronger future

Damaris Mills is its president Integrated DNA TechnologiesA global genomics solutions provider. getty Every fall, millions of Americans get their annual flu shots — and while they’re at it, they now also get their annual Covid-19 booster. Already, these shots seem routine; It’s easy to forget that the initial COVID-19…

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CRISPR/Cas9-mediated nexilin deficiency interferes with cardiac contractile function in zebrafish in vivo

CRISPR/Cas9-induced homozygous knockout of nexn causes progressive cardiac dysfunction without affecting skeletal muscle function in zebrafish Several studies in different animal models and cardiomyopathy patients have shown that loss of NEXN is leading to DCM which is characterized by an impaired contractility of the heart6,10,12. Homozygous loss of NEXN mostly…

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The science events to watch for in 2024

AI advances The rise of ChatGPT had a profound effect on science this year. Its creator, OpenAI in San Francisco, California, is expected to release GPT-5, the next generation of the artificial intelligence (AI) model that underpins the chatbot, late next year. GPT-5 is likely to showcase more advanced capabilities…

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Viral Vectors and Plasmid DNA Manufacturing market is

Visiongain has published a new report entitled Viral Vectors & Plasmid DNA Manufacturing Market Report 2024-2034: Forecasts by Vector Type (Adenovirus, Retrovirus, Plasmid DNA, AAV, Lentivirus, Others), by Application (Antisense and RNAi, Gene Therapy, Cell Therapy, Vaccinology), by End-use (Pharma and Biopharma Companies, Research Institutes), by Disease (Oncology, Genetic Disorders,…

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‘X-ray vision’ for investigation of mRNA nanomedicines

EMBL Staff Scientist Melissa Graewert together with two users from the Johannes Gutenberg University Mainz are performing measurements of RNA using small-angle X-ray scattering at the EMBL beamline P12 in Hamburg. Credit: Dorota Badowska/EMBL A new technology co-developed at EMBL Hamburg provides new insights into mRNA pharmaceuticals and other nanomedicines,…

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A super-pangenome of the North American wild grape species | Genome Biology

Alston JM, Sambucci O. Grapes in the world economy. In: Cantu D, Walker MA, editors. The grape genome. Springer International Publishing; 2019. p. 1–24. Google Scholar  Rahemi A, Dodson Peterson JC, Lund KT. Grape rootstocks and related species. Cham: Springer International Publishing; 2022. Walker MA, Heinitz C, Riaz S, Uretsky…

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How Synthetic Biology Is Building A Stronger Future

Demaris Mills is president at Integrated DNA Technologies, a global genomics solutions provider. getty Every fall, millions of Americans get their annual flu shots—and while they are at it, they now also get their annual Covid-19 boosters. Already, these shots seem routine; it is easy to forget that the initial…

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Synthetic Biology Industry Charts Course for a Remarkable US$138.8 Billion Valuation by 2032-FMI Study

Intradermal Injection Industry The value of the worldwide synthetic biology industry size was estimated at US$ 15.8 billion in 2022 and is projected to grow to around US$ 138.8 billion by 2032. Synthetic biology refers to redesigning organisms through engineering with the objective of improving development in health services and…

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Nine biotech companies to watch in 2024

As 2024 unfolds, the biotechnology industry is brimming with potential, spearheading scientific innovation. This year is particularly exciting as a diverse range of biotech companies are poised to break new ground to tackle global challenges. Here are nine biotech companies we will follow in 2024. Aiolos Bio Aiolos Bio has…

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Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2L

Loss of ASH2L prevents cell proliferation We have studied the molecular and cellular consequences of Ash2l loss in mouse embryo fibroblasts (MEFs) with floxed Ash2l alleles and an inducible Cre-ER recombinase (iMEF-Ash2lfl/fl-Cre-ER). While the knockout of Ash2l was rapid, the downstream effects, including the decrease in promoter-associated H3K4me3, altered gene…

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Plasma cell-free DNA 5-hydroxymethylcytosine and whole-genome sequencing signatures for early detection of esophageal cancer

Waters JK, Reznik SI. Update on management of squamous cell esophageal cancer. Curr Oncol Rep. 2022;24:375–85. Article  PubMed  Google Scholar  Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185…

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Results obtained by analyzing experiment data on mESCs in response to the varying E-P genomic distance.

(A) Distributions of the mRNA numbers of smRNA-FISH in different cell lines. The solid line shows the best fit of our stochastic multiscale model to the experimental data shown in the histograms. dG = NaN represents promoter-only control cell line. Parameter values are listed in Table E in S1 Text….

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First patient dosed in clinical trial of YOLT-201 for treatment of hereditary ATTR-CM

SHANGHAI, Dec. 17, 2023 /PRNewswire/ — Yoltech Therapeutics today announced that the first patient has been administered a dose of YOLT-201, the company’s first in vivo genome editing candidate being developed as a single dose, Which is a potentially curative therapy for hereditary transthyretin amyloidosis. With cardiomyopathy (ATTR-CM). The study…

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First Patient Dosed in Clinical Trial of YOLT-201 for the Treatment of Hereditary ATTR-CM

SHANGHAI, Dec. 17, 2023 /PRNewswire/ — YolTech Therapeutics today announced that the first patient has been dosed with YOLT-201, the company’s first in vivo genome editing candidate being developed as a single dose, potentially curative therapy for hereditary transthyretin amyloidosis with cardiomyopathy (ATTR-CM). The study by YolTech is a single-arm,…

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Orbivirus ~ ViralZone

VIRION Non enveloped, icosahedral, non-turreted virion with a triple capsid structure, about 80 nm in diameter. The intermediate capsid has a T=13 icosahedral symmetry, the inner capsid a T=2* icosahedral symmetry. Virion maturation schemes GENOME Segmented linear dsRNA genome. Contains 10 segments coding for 12 proteins. Segments size range from…

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Why does the Pair-End RNA library exhibit a significant disparity between the mapping results of R2 compared to R1?

Why does the Pair-End RNA library exhibit a significant disparity between the mapping results of R2 compared to R1? 0 We conducted total mRNA RNA-seq using Illumina technology and encountered significant disparity in the mapping between R2 and R1. We are considering potential issues with trimming; we utilized FastQC and…

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FDA Fails to Address DNA Adulteration Concerns

The following article originally appeared on Dr. Robert Malone’s Substack, and is reprinted here verbatim with permission. Robert Malone, MD, MS; Malone Institute December 15, 2023, 2:30 PM EST (Madison VA and Tallahassee FL) The failure of government regulatory authorities to identify and disclose DNA fragment contamination of…

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Randomized phase II study of preoperative afatinib in untreated head and neck cancers: predictive and pharmacodynamic biomarkers of activity

Study objectives and endpoints The main objective consisted in identifying predictive biomarkers of efficacy by exploring correlation between baseline potential biomarkers and radiological and metabolic responses to afatinib. Secondary objectives were to identify potential pharmacodynamic biomarkers, to evaluate the efficacy and safety of afatinib and to assess the metabolic and…

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Pharmalittle: FDA found problems at Moderna facility

Good morning, everyone, and welcome to another working week. We hope the weekend respite was relaxing and invigorating, because that familiar routine of online meetings and deadlines has predictably returned. But you knew this would happen, yes? To cope, we are firing up the coffee kettle and brewing another cup…

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What happens when cGPDH is treated with siRNA?

What is the time point for cell harvest post siRNA treatment via lipofectamine mediated transfection in adipocytes?4 answersThe time point for cell harvest post siRNA treatment via lipofectamine mediated transfection in adipocytes varies depending on the study. In one study by Kilroy et al., cells were harvested 48 hours post-transfection….

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Nucleic Acids: The Blueprint of Life

Nucleic acids are the building blocks of life, serving as the blueprint for the genetic information that is vital to all living organisms. While there are two main types of nucleic acids, deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), they each have unique functions in the cellular processes that drive…

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FDA Suggests Florida Surgeon General Is Spreading “Misinfo” About COVID Vax Safety

Florida Surgeon General Dr. Joseph Ladapo As the federal government renews calls for more COVID-19 vaccines, the Biden administration continues to argue that the jabs are safe and effective, and said suggestions to the contrary, such as those recently expressed by Florida’s top doctor, amount to “misinformation and disinformation” that…

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FDA Fails to Address DNA Adulteration Concerns, Resorting To Gaslighting Instead

Thanks, Jan! Lets get this amplified! Go Joe Ladapo! t.co/kgaIolmKnR — Robert W Malone, MD (@RWMaloneMD) December 16, 2023 Someone said: “If reports I’ve read in the news are correct, that many high level members of the FDA are heavily invested in the covid vaccines, then it make sense that…

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Lipid Nanoparticles (LNPs) CDMO Market Latest Trends and Future Opportunities Analysis to 2031

“Global Lipid Nanoparticles (LNPs) CDMO Market Focus on Nucleic Acids LNPs Segmented By Product (mRNA , Plasmid DNA (pDNA), siRNA, saRNA, microRNA, and Others), Scale Of Operation (Preclinical, Clinical , and Commercial), End-Users (Pharmaceutical Companies, Academic Research Institute, and Diagnostic Laboratories)- Trends, Industry Competition Analysis, Revenue and Forecast To 2030.”…

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Gadolinium Oxide Nanoparticles reinforce immune response

Boyi Yu,1– 4 Xuanyi Lu,5 Xianglong Feng,1– 4 Ting Zhao,1– 4 Jiaxin Li,1– 4 Yudie Lu,5 Fei Ye,1– 4 Xiongxiong Liu,1– 4 Xiaogang Zheng,1– 4 Zheyu Shen,5 Xiaodong Jin,1– 4 Weiqiang Chen,1– 4 Qiang Li1– 4 1Biomedical Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, People’s Republic of…

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False claim Moderna admitted mRNA vaccines cause cancer

Scientists win Nobel Prize for mRNA, COVID-19 work Dr. Katalin Karikó and Dr. Drew Weissman won the Nobel Prize in Physiology or Medicine for their work on mRNA vaccines, leading to a COVID vaccine. The claim: Moderna admitted mRNA vaccines cause cancer A Dec. 2 Instagram post (direct link, archive…

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FDA responds to Ladapo letter

In response to a letter in which Florida Surgeon General Joseph Ladapo noted the detection of DNA fragments in mRNA COVID-19 vaccines, Peter Marks, director of the U.S. Food and Drug Administration’s Center for Biologics Evaluation and Research, emphasized the FDA’s view that the shots are safe and effective. “I…

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The Role of RNA in Cellular Processes

RNA, a vital molecule found in all living cells, is essential for the synthesis of proteins, which are crucial for the functioning of our bodies. While DNA is well-known, RNA is equally significant in the process of gene expression. Let’s explore the diverse types of RNA and their roles within…

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Fatty Acid Metabolism-Related lncRNAs as Biomarkers for SKCM

Introduction Skin cutaneous melanoma (SKCM), as one of the most aggressive types of cancer due to its elevated degree of heterogeneity, has gained increasing attention during the past few decades.1 Also known as “the cancer that rises with the sun”,2 melanoma originates from cancerous melanocytes due to molecular or genetic…

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FDA official shames FL surgeon general for raising ‘DNA contamination’ concerns in COVID vaccines

Questioning the purity of mRNA COVID-19 vaccines is tantamount to spreading disinformation that kills people, the FDA’s top vaccines official not so subtly implied to Florida Surgeon General Joseph Ladapo this week. Ladapo notified FDA Commissioner Robert Califf and CDC Director Mandy Cohen Dec. 6 of recent research awaiting peer…

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COVID Vaccination after Infection Boosts Immune Response

Credit: matejmo/Getty Images People who received mRNA vaccines against SARS-CoV-2 after a COVID-19 infection produced a large number of protective CD8 T cells, researchers at the University of Washington School of Medicine in Seattle have found. Their work was published last week in Nature Immunology. The finding may explain why…

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Moderna Announces New England Journal of Medicine Publication of Pivotal Phase 3 Clinical Safety and Efficacy Data For MRNA-1345, The Company’s Investigational Respiratory Syncytial Virus (RSV) Vaccine

Moderna has submitted marketing authorizations globally for mRNA-1345 and continues to prepare for an expected 2024 marketing launch CAMBRIDGE, MA / ACCESSWIRE / December 15, 2023 / Moderna, Inc. (Nasdaq:MRNA) today announced the publication of positive Phase 3 clinical study results evaluating mRNA-1345, its vaccine candidate for respiratory syncytial virus…

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A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons

Animals All experimental procedures were performed in compliance with animal protocols approved by the Institutional Animal Care and Use Committee at Boston Children’s Hospital (Protocol no. 20-05-4165 R). Mice were provided with food and water ad libitum, housed on a 12-hour light/dark schedule (7 a.m.–7 p.m. light period) with no more than five mice…

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A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain

Mouse breeding and husbandry All experimental procedures related to the use of mice were approved by the Institutional Animal Care and Use Committee of the AIBS, in accordance with NIH guidelines. Mice were housed in a room with temperature (21–22 °C) and humidity (40–51%) control within the vivarium of the AIBS…

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Highly dynamic inflammatory and excitability transcriptional profiles in hippocampal CA1 following status epilepticus

Dynamic mRNA signatures in the early phase of epileptogenesis after Pilocarpine-induced SE To decipher transcriptional changes early after pilocarpine-induced SE in the hippocampal CA1 subfield, we compared mRNA expression profiles of pilocarpine-induced SE animals and non-SE controls in hippocampal CA1 at five different time points, i.e. 6, 12, 24, 36…

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Global DNA/RNA Extraction Industry aims for a consistent 7.4% CAGR during the period spanning 2023 to 2033 as per FMI’s analysis

PRESS RELEASE Published December 14, 2023 The global DNA/RNA extraction industry is on a trajectory of substantial growth, with estimations revealing promising projections. Future Market Insights’ latest report indicates that in 2022, the market boasted a value of US$ 1.3 billion, which surged to US$ 1.40 billion in 2023. Anticipated…

Continue Reading Global DNA/RNA Extraction Industry aims for a consistent 7.4% CAGR during the period spanning 2023 to 2033 as per FMI’s analysis

Morphogenesis, Inc. Announces Corporate Rebranding to TuHURA Biosciences, Inc. and Provides a Business Overview

Company focused on advancing novel personalized cancer vaccines to overcome tumor resistance to immunotherapies like checkpoint inhibitors; and first-in-class bi-functional antibody drug conjugates (ADCs) targeting Myeloid Derived Suppressor Cells (MDSCs) to modulate their immunosuppressive effects on the tumor microenvironment Company on track to initiate pivotal Phase 3 study of lead…

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Evolution of lysine-specific demethylase 1 and REST corepressor gene families and their molecular interaction

The RCOR gene repertoire expanded in the ancestor of jawed vertebrates To understand the duplicative history of the RCOR genes, we reconstructed gene phylogenies with different taxonomic samplings. The first analysis aimed to understand the evolution of RCOR genes in vertebrates (Fig. 2), whereas in the second, our sampling effort included…

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The latest on Moderna, Apellis, and Merck

Want to stay on top of the science and politics driving biotech today? Sign up to get our biotech newsletter in your inbox. Hello, everyone. Damian here with the fallout from Pfizer’s downbeat update, a new entrant in obesity research, and an orthogonal CRISPR business. The need-to-know this morning Apellis Pharmaceuticals said a…

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Their Roles and Interactome in Parkinson’s Disease Progression

Circular RNAs (circRNA) are products of alternative splicing that consist of a closed circular structure through a covalent 3′-5′ phosphodiester linkage. Their unique structure lends itself to a variety of physical properties and functions, including increased half-life within the cellular environment and the ability to sponge microRNA (miRNA) and RNA-binding…

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Cancer proteogenomics pave the way for precision medicine

DNA provides the instruction manual for making proteins. This fundamental scientific tenet is a staple lesson in introductory biology classes everywhere. It is less widely taught, however, that only approximately one percent of the genome actually serves as a template for making proteins (1). While scientists previously considered the remaining…

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read the article to learn more about the types of rna specifically mrna and trna. use the information to answer the following question. rna explain the roles of mrna and trna in protein synthesis.

read the article to learn more about the types of rna specifically mrna and trna. use the information to answer the following question. rna explain the roles of mrna and trna in protein synthesis. Chapter 7 RNA and the Genetic Code Flashcards | Quizlet Gene. unit of DNA that encodes…

Continue Reading read the article to learn more about the types of rna specifically mrna and trna. use the information to answer the following question. rna explain the roles of mrna and trna in protein synthesis.

Seven precision medicine companies worth keeping an eye on

Precision medicine is an approach to tailoring treatments based on people’s genetic makeup, lifestyles and environment. These targeted interventions can tackle diseases like cancer, neurological as well as metabolic diseases. The global market value of the precision medicine industry, encompassing many companies, crossed 70 billion last year, and is expected…

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CDC director visits Orlando to encourage vaccination, combat misinformation

Centers for Disease Control and Prevention Director Dr. Mandy Cohen traveled to Orlando this week as part of a nationwide tour to combat vaccine misinformation during respiratory disease season. Doctors this year are recommending a flu vaccine, an updated COVID-19 booster, and, for those who qualify, the newly available vaccine…

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What is the mechanism of action of Irf5 siRNA-loaded biodegradable lipid nanoparticles?

How can lipid particles for RNA delivery be prepared by self-assembly?3 answersLipid particles for RNA delivery can be prepared by self-assembly through the use of lipid nanoparticles (LNPs). LNPs are lipid vesicles with a homogeneous lipid core that protect mRNA from degradation and assist with intracellular delivery. The self-assembly of…

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Zafrens Plans to Advance Multimodal Drug Discovery Platform with $23M Financing

Although he started out in theoretical physics, Swamy Vijayan, PhD, has worked in the biotechnology industry for most of his professional career. His path included a stint at Illumina and the launch of Omniome, a short read sequencing company that was acquired by Pacific Biosciences in 2021. His next gig…

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Bioactive glycans in a microbiome-directed food for children with malnutrition

Collection and handling of biospecimens obtained from participants in the randomized controlled clinical study of the efficacy of MDCF-2 The human study entitled ‘Community-based clinical trial with microbiota-directed complementary foods (MDCFs) made of locally available food ingredients for the management of children with primary moderate acute malnutrition (MAM)’ was approved…

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Solved The genome of negative, mRNA positive, rRNA positive,

Science Biology Biology questions and answers The genome of negative, mRNA positive, rRNA positive, mRNA negative, rRNA-sense RNA viruses are the functional equivalent of The genome of negative, mRNA positive, rRNA positive, mRNA negative, rRNA-sense RNA viruses are the functional equivalent of Expert Answer Who are the experts?Experts are tested…

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Solved The ribosome is a large complex of ribosomal RNA

The ribosome is a large complex of ribosomal RNA (rRNA) and proteins. It consists of two subunits. The smaller subunit binds to the mRNA strand and the larger subunit holds the tRNA molecules in place while the covalent peptide bond is formed between the amino acids. Several ribosomes can attach…

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Cas9-induced targeted integration of large DNA payloads in primary human T cells via homology-mediated end-joining DNA repair

Khan, S. et al. Role of recombinant DNA technology to improve life. Int. J. Genomics Proteomics 2016, 2405954 (2016). Google Scholar  Spolski, R., Li, P. & Leonard, W. J. Biology and regulation of IL-2: from molecular mechanisms to human therapy. Nat. Rev. Immunol. 18, 648–659 (2018). Article  CAS  PubMed  Google…

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Merck and Moderna Initiate INTerpath-002, a Phase 3 Study Evaluating V940 in Combination with KEYTRUDA for Adjuvant Treatment of Patients with Certain Types of Resected Non-Small Cell Lung Cancer -December 12, 2023 at 06:22 am EST

RAHWAY – Merck (NYSE: MRK), known as MSD outside of the United States and Canada, and Moderna, Inc. (Nasdaq: MRNA), today announced the initiation of INTerpath-002, a pivotal Phase 3 randomized clinical trial evaluating V940 (mRNA-4157), an investigational individualized neoantigen therapy (INT), in combination with KEYTRUDA, Merck s anti-PD-1 therapy,…

Continue Reading Merck and Moderna Initiate INTerpath-002, a Phase 3 Study Evaluating V940 in Combination with KEYTRUDA for Adjuvant Treatment of Patients with Certain Types of Resected Non-Small Cell Lung Cancer -December 12, 2023 at 06:22 am EST

RNAi Technology Market is expected to reach US$ 3.6 billion by 2030

The field of RNA interference (RNAi) is witnessing significant growth with the approval of small interfering RNA (siRNA) drugs by the US FDA. These siRNA therapeutics offer a powerful and specific approach to target mRNA, with notable approvals like Patisiran, Givosiran, Lumasiran, Inclisiran, and Vutrisiran. The liver has been a…

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Exclusive interview: Florida Surgeon General Joseph Ladapo asks FDA for data on risks of DNA contaminants in COVID-19 vaccines

Florida Surgeon General Joseph Ladapo | Photo courtesy of FDOH BY JENNIFER CABRERA TALLAHASSEE, Fla. – On December 6, Florida Surgeon General Joseph Ladapo sent a letter to the U.S. Food and Drug Administration (FDA), asking questions about a recent preprint documenting the detection of billions of DNA fragments per…

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Atg7 senses ATP levels and regulates AKT1-PDCD4 phosphorylation-ubiquitination axis to promote survival during metabolic stress

Atg7 interacts with PDCD4 and negatively regulates PDCD4 protein levels Previous studies have demonstrated the role of Atg7 in maintaining metabolic homeostasis by initiating autophagy and arresting the cell cycle17,18,22. Herein, the function of Atg7 was more comprehensively investigated and it was determined whether Atg7 reduces protein synthesis to save…

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An ncRNA transcriptomics-based approach to design siRNA molecules against SARS-CoV-2 double membrane vesicle formation and accessory genes | BMC Infectious Diseases

When compared to the genomes of other RNA viruses, coronaviruses have been found to possess largest genome sizes. They are capable of establishing reservoirs in both human and zoonotic populations, enabling their transmission and circulation among a range of animal hosts, including bats, pangolins, civets, cats, mice, pigs, whales, dogs,…

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Regulation of the ER-Resident Mannosidase EDEM2 in HEK293 Cells

Abstract EDEM2 plays an important role as the first enzyme that acts during mannose trimming of N-glycosylated proteins in the ERAD machinery. Although EDEM2 expression has been reported to be transcriptionally regulated by the IRE1-sXBP1 pathway, very little is known about how endogenous EDEM2 protein expression is regulated. In this…

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Methylation Analysis Tutorial in R_part1

The code and approaches that I share here are those I am using to analyze TCGA methylation data. At the bottom of the page, you can find references used to make this tutorial. If you are coming from a computer background, please bear with a geneticist who tried to code…

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Kinnex launch promises to revolutionize RNA research

RNA sequencing (RNA-seq) has become an indispensable tool for analyzing transcriptomes across all domains of life that can reveal insights about biology and disease. While the genome remains relatively constant for most species over brief time scales, the transcriptome –the sum total of expressed messenger RNA (mRNA) transcripts – varies…

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Phylogeny and molecular evolution of the first local monkeypox virus cluster in Guangdong Province, China

Phylogenetic analysis of lineage IIb C.1 containing all ten local MPXV from Guangdong To rapidly characterize the phylogeny of the MPXVs involved in the outbreak in Guangdong, we constructed a phylogenetic tree by integrating 10 MPXV sequences from Guangdong into the global MPXV genetic diversity sequences derived from GISAID (Fig. 1a,…

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Solved Answer questions 37-39 based on the options (A, B, C,

Transcribed image text: Answer questions 37-39 based on the options (A, B, C, or D) below. Each graph represents the RNAseq (top) and Ribo-seq (bottom) reads aligned to a reference gene. 37) Which graph represents the mRNA that is generating more protein? 38) Which graph represents an mRNA without introns?…

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Panel-based RNA fusion sequencing improves diagnostics of pediatric acute myeloid leukemia

Rasche M, Zimmermann M, Borschel L, Bourquin J, Dworzak M, Klingebiel T, et al. Successes and challenges in the treatment of pediatric acute myeloid leukemia: a retrospective analysis of the AML-BFM trials from 1987 to 2012. Leukemia. 2018;32:2167–77. Article  PubMed  PubMed Central  Google Scholar  Manola KN. Cytogenetics of pediatric acute…

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Supreme Court Throws Out Cases On Covid Shot Mandates For Troops

The federal employee vaccine requirement was rescinded by President Joe Biden in May, and the Pentagon similarly withdrew a mandate for troops, but cases challenging the mandate for executive branch employees and military service members had rumbled on. CNN: Covid-19 Vaccine Mandates For Executive Branch Employees And Troops Thrown Out…

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