Tag: msa

alphafold2: HHblits failed – githubmemory

I’ve tried using the standard alphafold2 setup via docker (converted to a singularity container) via the setup described at github.com/kalininalab/alphafold_non_docker, and both result in the following error: […] E1210 12:01:01.009660 22603932526400 hhblits.py:141] – 11:49:18.512 INFO: Iteration 1 E1210 12:01:01.009703 22603932526400 hhblits.py:141] – 11:49:19.070 INFO: Prefiltering database E1210 12:01:01.009746 22603932526400 hhblits.py:141]…

Continue Reading alphafold2: HHblits failed – githubmemory

FastTree error while constructing tree

Hey All, I am trying to infer a phylogeny from a multiple sequence alignment using FastTree program, however the program is giving me an error when I run it over the multiple sequence alignment and I can not figure out what the error is saying (not really that informative). My…

Continue Reading FastTree error while constructing tree

Aligning large sets of sequences

Hello folks, I am seeking an advice on Multiple Sequence Alignment that I am trying to get. The fasta file i am trying to align belongs to Sars-Cov-2 Spike protein, it has nearly 600k sequences and ranges from 1270-1275 aa. I have aligned with clustalo and mafft with default parameters….

Continue Reading Aligning large sets of sequences

deepmind alphafold github

The program handily beat all competitors, in what one . This will allow us to run alphafold only using CPU ( which is what our VM has). From the developers’ original publication: “The provided inference . The AlphaFold method. Found insideThis book constitutes the refereed proceedings of the First International…

Continue Reading deepmind alphafold github

Install alphafold on the local machine, get out of docker.

AlphaFold This package provides an implementation of the inference pipeline of AlphaFold v2.0. This is a completely new model that was entered in CASP14 and published in Nature. For simplicity, we refer to this model as AlphaFold throughout the rest of this document. Any publication that discloses findings arising from…

Continue Reading Install alphafold on the local machine, get out of docker.

alphafold colab github

for the third time worked! Found inside – Page iiThe eight-volume set comprising LNCS volumes 9905-9912 constitutes the refereed proceedings of the 14th European Conference on Computer Vision, ECCV 2016, held in Amsterdam, The Netherlands, in October 2016. Please make sure you have a large enough hard drive space, bandwidth…

Continue Reading alphafold colab github

highly accurate protein structure prediction with alphafold

In: StatPearls [Internet]. team at our virtual booth on the exhibitor tab. Published online 22 07; DOI: 10.1038/s41586-021-03819-2. Extended Data Fig. Found insideAn important feature of this work is the S-plus subroutines provided for analyzing actual data sets. Coupled with the discussion of new theoretical research, the book should benefit…

Continue Reading highly accurate protein structure prediction with alphafold

Difficulty installing Bioperl for use with GUIDANCE

Difficulty installing Bioperl for use with GUIDANCE 0 Hi, I am trying to use the Guidance software to analyze a set of 427 sequences. I have the software downloaded and installed the modules indicated in the Guidance user guide (Perl/BioPerl/Ruby), but when I attempt to run Guidance with the following…

Continue Reading Difficulty installing Bioperl for use with GUIDANCE

Pact_00210 : CDS information — DoBISCUIT

Category 3.4 other modification Product putative 6-methylsalicylyltransferase Product (GenBank) ketoacyl-ACP synthase Gene pctTptmR Gene (GenBank) pctT EC number Keyword Note Note (GenBank) Reference ACC A8R0K3 PmId [17827660] Cloning of the pactamycin biosynthetic gene cluster and characterization of a crucial glycosyltransferase prior to a unique cyclopentane ring formation. (J Antibiot (Tokyo)….

Continue Reading Pact_00210 : CDS information — DoBISCUIT

SCIRP Open Access

Add your e-mail address to receive free newsletters from SCIRP. Select Journal AA AAD AAR AASoci AAST ABB ABC ABCR ACES ACS ACT AD ADR AE AER AHS AID AiM AIT AJAC AJC AJCC AJCM AJIBM AJMB AJOR AJPS ALAMT ALC ALS AM AMI AMPC ANP APD APE APM ARS ARSci AS ASM BLR CC CE CellBio ChnStd CM CMB CN CRCM CS CSTA CUS CWEEE Detection EMAE ENG EPE ETSN FMAR FNS GEP GIS GM Graphene GSC Health IB ICA IIM IJAA IJAMSC IJCCE IJCM IJCNS IJG IJIDS IJIS IJMNTA IJMPCERO IJNM IJOC IJOHNS InfraMatics JACEN JAMP JASMI JBBS JBCPR JBiSE JBM JBNB JBPC JCC JCDSA JCPT JCT JDAIP JDM JEAS JECTC JEMAA JEP JFCMV JFRM JGIS JHEPGC JHRSS JIBTVA JILSA JIS JMF JMGBND JMMCE JMP JPEE JQIS JSBS JSEA JSEMAT JSIP JSS JSSM JST JTR JTST JTTs JWARP LCE MC ME MI MME MNSMS MPS MR MRC MRI MSA MSCE NJGC NM NR NS OALib OALibJ ODEM OJA OJAB OJAcct OJAnes OJAP OJApo OJAppS OJAPr OJAS OJBD OJBIPHY OJBM OJC OJCB OJCD OJCE OJCM OJD OJDer OJDM OJE OJEE OJEM OJEMD OJEpi OJER OJF OJFD OJG OJGas OJGen OJI OJIC OJIM OJINM OJL OJM OJMC OJMetal OJMH OJMI OJMIP OJML OJMM OJMN OJMP OJMS OJMSi OJN OJNeph OJO OJOG OJOGas OJOp OJOph OJOPM OJOTS OJPathology OJPC OJPChem OJPed OJPM OJPP OJPS OJPsych OJRA OJRad OJRD OJRM OJS OJSS OJSST OJST OJSTA OJTR OJTS OJU OJVM OPJ POS PP PST PSYCH SAR SCD SGRE SM SN SNL Soft SS TEL TI UOAJ VP WET WJA WJCD WJCMP WJCS WJET WJM WJNS WJNSE WJNST WJV WSN YM Read more here: Source link

Continue Reading SCIRP Open Access

Error while running MEGAX

Error while running MEGAX 0 I am running the latest version of MEGA i.e MEGAX. I am trying to run the command megacc but it is throwing an error. My sequences.fas file contains the trimmed multiple sequence alignment (MSA) of 16S rRNA sequences of 1403 organisms in a fasta format….

Continue Reading Error while running MEGAX

bcftools merge; retaining sample names

bcftools merge; retaining sample names 2 When I do bcftools merge, the headers do not retain the filenames.  How can I specify filenames? This is my command  bcftools merge vcf/unfiltered/*.vcf.gz -O z > msa/pooled.vcf.gz However this is the relevant part of my header, despite the filenames I gave it.  Is…

Continue Reading bcftools merge; retaining sample names

Remove non variable sites from sequence alignment

Remove non variable sites from sequence alignment 0 I’m looking for suggestions for programs that will remove nonvariable sites from a fasta/phylip/nexus alignment. I have very large sequence alignments, and I am hoping only to retain variable sites. MSA alignment nexus FASTA sequence • 12 views Login before adding your…

Continue Reading Remove non variable sites from sequence alignment

keep getting error with psiblast

keep getting error with psiblast 1 hello I am trying to calculate the PSSM but I keep getting error , where is the problem in this code ? import os import re def command_pssm(content, output_file,pssm_file): os.system(‘psiblast -in_msa 1ak4.fasta -db allseq.fasta -num_threads 10 -num_iterations 3 -evalue 0.001 -out output_file -out_ascii_pssm PSSM.txt’…

Continue Reading keep getting error with psiblast

alphafold online availability and use case

I’m new to both protein structure prediction and the use of AI-based tools like Alphafold2 or RoseTTAFold. And I have a few questions: **1.** Is it possible to use structure prediction by AlphaFold2 to **validate** HMMER based domain sequence predictions? If yes, what would be the steps? I have some…

Continue Reading alphafold online availability and use case

Filtering MSA by similiarity to a consensus sequence/motif

Filtering MSA by similiarity to a consensus sequence/motif 0 Dear all, anyone knows a good way of filtering or sorting a large multiple sequence alignment (~8000 sequences) by similarity to a given consensus sequence? A solution using python/biopython would be optimal. Any help is appreciated! Best Jonathan biopython motif multiple…

Continue Reading Filtering MSA by similiarity to a consensus sequence/motif

How to visualise a phylogenetic tree with amino acids (double letter repeat) multiple-sequence alignment?

How to visualise a phylogenetic tree with amino acids (double letter repeat) multiple-sequence alignment? 0 I have a fasta file as shown below, rvd.fasta >t1 NI-NG-NR-NN-NG-HD-HD >t_temp5 NG-NG-NI-N*-NR-NI-NN-NG-NG-HD >tal8 NG-NG-NI-N*-ND-NI-NN-NG-NG-H*-NH-NI I have a newick file as follows, tree.newick (tal8:0.49999997,t_temp5:0.47298786,t1:28.37858179); I need to visualise both the tree and rvd.fasta file (multiple-sequence…

Continue Reading How to visualise a phylogenetic tree with amino acids (double letter repeat) multiple-sequence alignment?