Tag: MULTI-seq

Researchers explore immunomodulating targets for improving glioblastoma therapies

In a recent article published in EMBO Molecular Medicine, researchers investigated cellular, molecular, and spatiotemporal heterogeneity of glioblastoma (GBM), a malignant and highly aggressive primary brain tumor. Study: Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastoma. Image Credit: Triff/Shutterstock.com They performed single-cell ribonucleic acid sequencing (scRNA-seq) of…

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Multiplexed transcriptomic profiling of the fate of human CAR T cells in vivo via genetic barcoding with shielded small nucleotides

Wagner, A., Regev, A. & Yosef, N. Revealing the vectors of cellular identity with single-cell genomics. Nat. Biotechnol. 34, 1145–1160 (2016). Article  CAS  PubMed  PubMed Central  Google Scholar  Gehring, J., Hwee Park, J., Chen, S., Thomson, M. & Pachter, L. Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular…

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Highlights from the 2023 Single-Cell and AI in Medicine Symposium

By Randall C. Willis with review and comments from Fabrice Chouraqui, Laurens Kruidenier, Chad Nusbaum, Diogo Camacho  August 2, 2023 | “Technology and computation are revolutionizing the way we are creating and developing new drugs,” Cellarity CEO Fabrice Chouraqui announced to open the Second Single-Cell and AI in Medicine Symposium…

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MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing

Single-cell RNA-seq (scRNA-Seq) is a powerful but relatively low-throughput tool to analyze gene expression at the single-cell level. Discover our new MULTI-seq system, developed to multiplex sample types using lipid-modified oligonucleotides (LMOs) complexed with unique DNA sample barcodes, allowing for multiple samples to be pooled together in the same single-cell…

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Count SNP per read

Count SNP per read 0 Hello, I have 6 samples of near-whole length sequenced hiv fasta files. The samples cover three different time points and there is a gene +/- sample per time-point. The samples have different numbers of reads of around ~8000 bp. I have performed a multi-seq alignment…

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A genome-scale screen for synthetic drivers of T cell proliferation

Abramson, J. S. et al. Transcend NHL 001: immunotherapy with the CD19-directed CAR T-cell product JCAR017 results in high complete response rates in relapsed or refractory B-cell non-Hodgkin lymphoma. Blood 128, 4192–4192 (2016). Google Scholar  Shifrut, E. et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators…

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