Categories
Tag: MULTI-seq
Researchers explore immunomodulating targets for improving glioblastoma therapies
In a recent article published in EMBO Molecular Medicine, researchers investigated cellular, molecular, and spatiotemporal heterogeneity of glioblastoma (GBM), a malignant and highly aggressive primary brain tumor. Study: Single-cell profiling and zebrafish avatars reveal LGALS1 as immunomodulating target in glioblastoma. Image Credit: Triff/Shutterstock.com They performed single-cell ribonucleic acid sequencing (scRNA-seq) of…
Multiplexed transcriptomic profiling of the fate of human CAR T cells in vivo via genetic barcoding with shielded small nucleotides
Wagner, A., Regev, A. & Yosef, N. Revealing the vectors of cellular identity with single-cell genomics. Nat. Biotechnol. 34, 1145–1160 (2016). Article CAS PubMed PubMed Central Google Scholar Gehring, J., Hwee Park, J., Chen, S., Thomson, M. & Pachter, L. Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular…
Highlights from the 2023 Single-Cell and AI in Medicine Symposium
By Randall C. Willis with review and comments from Fabrice Chouraqui, Laurens Kruidenier, Chad Nusbaum, Diogo Camacho August 2, 2023 | “Technology and computation are revolutionizing the way we are creating and developing new drugs,” Cellarity CEO Fabrice Chouraqui announced to open the Second Single-Cell and AI in Medicine Symposium…
MULTI-seq Sample Multiplexing for Single Cell Analysis and Sequencing
Single-cell RNA-seq (scRNA-Seq) is a powerful but relatively low-throughput tool to analyze gene expression at the single-cell level. Discover our new MULTI-seq system, developed to multiplex sample types using lipid-modified oligonucleotides (LMOs) complexed with unique DNA sample barcodes, allowing for multiple samples to be pooled together in the same single-cell…
Count SNP per read
Count SNP per read 0 Hello, I have 6 samples of near-whole length sequenced hiv fasta files. The samples cover three different time points and there is a gene +/- sample per time-point. The samples have different numbers of reads of around ~8000 bp. I have performed a multi-seq alignment…
A genome-scale screen for synthetic drivers of T cell proliferation
Abramson, J. S. et al. Transcend NHL 001: immunotherapy with the CD19-directed CAR T-cell product JCAR017 results in high complete response rates in relapsed or refractory B-cell non-Hodgkin lymphoma. Blood 128, 4192–4192 (2016). Google Scholar Shifrut, E. et al. Genome-wide CRISPR screens in primary human T cells reveal key regulators…