Categories
Tag: NA
UMI workflow resulting in bams with empty reads
Hello all, In my NGS workflow for UMI based reads, I first tried identifying and removing sequence adapters using bbmerge and cutcadapt: BBMERGE -Xmx1g -ignorejunk in1=SAMPLE_R1 in2=SAMPLE_R2 outa= adapters.fa itn CUTADAPT -a forward_adapter -A reverse_adapter -o s_2_1_sequence_trimmed_UN.fastq.gz -p s_2_2_sequence_trimmed_UN.fastq.gz SAMPLE_R1 SAMPLE_R2 Then, I converted the trimmed fastq files to an…
Unveiling Roman Amphitheaters with a ggplot2 violin plot
[This article was first published on coding-the-past, and kindly contributed to R-bloggers]. (You can report issue about the content on this page here) Want to share your content on R-bloggers? click here if you have a blog, or here if you don’t. 1. What is a violin plot? A violin…
r – How can I change position x-axis ticks in ggplot of regression
Having completed my regression equation and F-statistic I have been able to plot a regression curve with confidence limits through my set of data points which I am happy with. My x variable is number of days from start of observations which runs for exactly one year (17 Jan 2009…
r – Clustered standard error bars for two-way bar chart in ggplot
I am looking for help with code for adding clustered standard errors to a two-way bar chart generated using ggplot. All of the packages I find are for regression results. I have data in which 1200 respondents each rated 10 profiles (n=12,000). I want to plot the means (Y) by…
Dryad | Data — Trait-based mechanistic approach highlights global patterns and losses of herbivore biomass functional diversity
README: Trait-based mechanistic approach highlights global patterns and losses of herbivore biomass functional diversity Purpose of data: the dataset contains species trait data used for creation of Herbivore Functional Types and density data used for calculating empirical-derived biomass. Authors: F Berzaghi*, D Zhu, J Alroy, P Ciais. *contact author: fabe@unitus.it…
r – Move positioning of x axis labels in ggplot without hjust or vjust
Using some sample data from BLS below, I’m trying to make a faceted plot of spending categories by income bracket. Because some of the label names are long, I have them rotated 90 degrees. I have been using hjust and vjust to move around the labels a bit, but I…
ggplot2 – Changing legend title in ggpattern R
You have both the fill and the pattern aesthetic mapped to the same variable, so you can combine the legends simply by giving these aesthetics the same names. You didn’t include kata_difilter or ftj in your question, so the plot is not fully reproducible, but I have recreated a modified…
r – Keeping unused levels in ggplot2 bar plot does not work properly
R version 4.3.1 library(ggplot2) version 3.4.2 I want to use ggplot2 bar charts to display the percentage frequency of a particular concentration class. I have five concentration classes (=c1 with the options “1_”, “2_”, “3_”, “4_”, “5_”) for which an event can occur “Early” (=c2 dichotomous with “yes” or “no”)…
r – ggplot2 – How to plot column with multiple dtypes?
I am trying to plot some data relating to carbon storage using ggplot2 in R. I am able to plot each data feature against the target variable ‘carbon’, but when I do so there are so many ticks on the x axis that the numbers become illegible. The issue lies…
Complete Tutorial Guide / AvaxHome
Parallel Computing With R & Rstudio: Complete Tutorial GuidePublished 12/2023MP4 | Video: h264, 1920×1080 | Audio: AAC, 44.1 KHzLanguage: English | Size: 1.30 GB | Duration: 1h 30m R Programming, RStudio, Parallel Computing, Multicore, Data Science, Speed Optimization What you’ll learn Understand core parallel computing concepts. Explore essential R packages…
Error in CIBERSORTx
Hello, I am trying to use CIBERSORT to deconvolute the immune cells in pancreatic cancer after my treatments. I have 3 biological replicates of Control, Treatments A,B,C. Using edgeR, I created the cpm matrix which is not log transformed. and converted it to the required format as follows: # Load…
Effects of electroporation on the transmembrane potential distribution in a two-dimensional bidomain model of cardiac tissue.
Effects of electroporation on the transmembrane potential distribution in a two-dimensional bidomain model of cardiac tissue. Publication , Journal Article Aguel, F; Debruin, KA; Krassowska, W; Trayanova, NA Published in: Journal of cardiovascular electrophysiology Defibrillation shocks, when delivered through internal electrodes, establish transmembrane potentials (Vm) large enough to electroporate the membrane…
No genes mapped in clusterprofiler gseGO
Hello! I’m having issues generating an adequate geneList for running gseGO in clusterProfiler, using keytype = “GO” Similar issues have been described here: No gene mapped gseGO code is: gse <- gseGO(geneList = gene_List, ont = “ALL”, #ont one of “BP”, “MF”, “CC” or “ALL” OrgDb = OrgDb, minGSSize =…
Electroporation Instruments Market Size, Growth and Forecast from 2023
Electroporation Instruments Market Analysis and Latest Trends Electroporation instruments are devices used to introduce substances into cells by using an electric pulse. This technique is commonly used in molecular biology and biotechnology for gene delivery, gene therapy, and drug delivery applications. Electroporation instruments are widely used in research laboratories, academic…
Functional-metabolic coupling in distinct renal cell types coordinates organ-wide physiology and delays premature ageing
Preferential carbohydrate import and early metabolism in PCs (but not SCs) supports renal physiology independent of ATP production Drosophila renal (Malpighian, MpT) tubules consist of two major cell types, the larger PCs and smaller SCs which perform distinct roles in ion, solute and water transport (Fig. 1a, b)16. These functional differences…
ggplot2 – How to make a percentage plot histogram in R / ggplot
Suppose your data looks something like this: set.seed(2) df <- data.frame(SBP = sample(101:199, 1000, TRUE)) df$survived <- c(‘yes’, ‘no’)[rbinom(1000, 1, (df$SBP – 100)/200) + 1] head(df) #> SBP survived #> 1 185 no #> 2 179 yes #> 3 170 no #> 4 106 yes #> 5 132 yes #>…
Batch and Sample correction for downstream analysis using DESeq2
Hello everyone, I am an absolute beginner on sequencing analysis and DESeq2, so please forgive me for possibly mundane questions. I have tried to look up different methods, but couldn’t find a fitting answer yet. I am currently working with sequencing data derived from an Illumina sequencer. The data is…
Single-cell RNA-seq workflow
In this tutorial we walk through a typical single-cell RNA-seq analysis using Bioconductor packages. We will try to cover data from different protocols, but some of the EDA/QC steps will be focused on the 10X Genomics Chromium protocol. We start from the output of the Cell Ranger preprocessing software. This…
Delete or Drop rows in R with conditions
Drop rows in R with conditions can be done with the help of subset () function. Let’s see how to delete or drop rows with multiple conditions in R with an example. Drop rows with missing and null values is accomplished using omit(), complete.cases() and slice() function. Drop rows by…
Kaggle X – na this aint got any description – Hurdles: While talking about hurdles the first
Hurdles: While talking about hurdles the first difficulty comes at the computational level as data size in practical life id ever increasing thus limited extent of cpu and gpu functionality plays a big role also due to this the cost and time for data ingestion , data cleaning increases considerably…
DESeq2 interaction term + sva
Hello, I am performing DGE analysis using DESeq2. I have two groups to compare: CTRL and SA, and I have performed a group comparison using DESeq2 and there’s no issue with that. However, I have males and females in each group, and I’m curious to see if there’s an interaction…
stat_quant_band function – RDocumentation
ggplot(mpg, aes(displ, hwy)) + geom_point() + stat_quant_band() # If you need the fitting to be done along the y-axis set the orientation ggplot(mpg, aes(displ, hwy)) + geom_point() + stat_quant_band(orientation = “y”) ggplot(mpg, aes(displ, hwy)) + geom_point() + stat_quant_band(formula = y ~ x) ggplot(mpg, aes(displ, hwy)) + geom_point() + stat_quant_band(formula =…
adding pvalues in regression Rstudio
results <- data.frame(prov = rownames(reg1$coefficients), b = reg1$coefficients, se = 0) results$se[!is.na(reg1$coefficients)] <- reg1$se results This shows the coefficients and the se… how can I make that this shows also the pvalues?? prov b se ln_Y ln_Y 1.028167431 0.02080551 ln_E ln_E 1.031713536 0.01992422 ln_DIST ln_DIST -0.667898564 0.03304988 CNTG CNTG 0.438520721…
facet grid – ggplot facet_grid: need lines between outside strip placement and panels
I am using facet_grid to create a plot with one column and multiple rows, with an outside strip placement to the left of the y-axis tick mark labels. I would like to enclose the y-axis tick mark labels such that each facet strip label is cleanly attached to its associated…
ggplot2 – Adding a box around a caption in R
One option would be to switch to ggtext::element_markdown which has a lot more options than element_text and allows to for an outline: library(ggplot2) library(ggtext) df <- as.data.frame(data(“iris”)) ggplot(data = iris, aes(x = Species, y = Petal.Width)) + geom_bar(stat = “identity”, position = “identity”) + labs(caption = “Add box around me”)…
CD CHO Media Market Size Reveals the Best Marketing Channels In Global Industry | by Evalynkoepp | Dec, 2023
CD CHO Media Market Trends, Growth Opportunities, and Forecast Scenarios The CD CHO media market is experiencing significant growth and is expected to continue expanding in the coming years. CD CHO media, also known as chemically-defined cell culture media for Chinese hamster ovary (CHO) cells, is widely used in biopharmaceutical…
how to get sequences by location when pyfaidx.Fasta(read_long_names=True) creates keys from FastaRecord (dmel r6 genome build)?
I’m attempting to use pyfaidx to index the dmel r6 genome build so that I can get actual sequences from tuples like (chromosome, start,end). In the pyfaidx documentation, they describe this process using pyfaidx.Fasta, which is able to access sequences using discrete chromosome locations: >>> genes = Fasta(‘tests/data/genes.fasta’) >>> genes[‘NM_001282543.1’][200:230]…
Google Data Analytics-Study Case 1:RStudio & Tableu | by Assyifa Nur Aziza | Dec, 2023
Recently, I completed Google Data Analytics Professional by Coursera. The course is challenging and exciting at the same time. However, it is worth studying. The course consists of 8 chapters, and at the end of the chapter, there are several case studies to solve. This article will consist of a…
Identification of Differentially Expressed Genes in Human Colorectal Cancer Using RNASeq Data Validated on the Molecular Level with Real-Time PCR
Allam RM, Al-Abd AM, Khedr A, Sharaf OA, Nofal SM, Khalifa AE, Mosli HA, Abdel-Naim AB (2018) Fingolimod interrupts the cross talk between estrogen metabolism and sphingolipid metabolism within prostate cancer cells. Toxicol Lett 291:77–85 Article CAS PubMed Google Scholar Andrews S et al (2010) FastQC: a quality control tool…
ggplot2 – Should I update my ggplot code in R version 4.3.1?
I made code for a boxplot in R version 3.6.1: ggplot(Data_Addi, aes(x=as.factor(Sex), y=DEFS_SF, fill=as.factor(Diagnosis_Addison_Recoded))) + geom_boxplot(outlier.shape=NA, color=”black”) + geom_jitter(position=position_jitterdodge(),show.legend = FALSE) + theme_bw(base_size = 25) + scale_x_discrete(name=””, breaks=c(“1”, “2”),labels=c(“Females”, “Males”)) + scale_fill_manual(name=”Diagnostic Group”, breaks=c(“1”, “2”), labels=c(“Addison”,”Control”), values=c(“white”, “grey”)) + labs(title=”Executive function total”, x=”Sex”, y=”BDEFS-SF Total Score”) This gave the following…
Formononetin | FGFR2 inhibitor | Axon 4067
All All Products Enzymes Receptors Ion Channels Research Areas Oxidoreductases Transferases Lyases Isomerases Ligases Nuclear Receptors Ligand-gated ATP-Sensitive Angiogenesis Apoptosis Cardiovascular Cell Cycle Regulation Cell Signaling & Oncology CNS Diabetes & Metabolism Epigenetics Immunology Miscellaneous Pain & Inflammation Stem Cell Oxidases MAO Cys-loop, cationic Cis-Trans Isomerase CypA DNA Topoisomerase Synthetases…
Wrangling And Analyzing Data In RStudio
Article Summary Box Efficient data preparation in RStudio hinges on automated data cleaning techniques, significantly reducing manual errors and streamlining the initial stages of analysis. Vectorized operations and the apply() family functions in RStudio dramatically enhance data manipulation efficiency, especially for large datasets. Utilizing multiple linear regression and PCA in…
Importing Data In RStudio: A Step-By-Step Approach
Article Summary Box Recognizing various data types like numeric, integer, and logical in RStudio is essential for accurate data manipulation and import. Effective environment setup, including package installation and global option configuration, is pivotal for streamlined data import processes. Utilizing data.table’s fread function for handling large datasets enhances import efficiency,…
A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons
Animals All experimental procedures were performed in compliance with animal protocols approved by the Institutional Animal Care and Use Committee at Boston Children’s Hospital (Protocol no. 20-05-4165 R). Mice were provided with food and water ad libitum, housed on a 12-hour light/dark schedule (7 a.m.–7 p.m. light period) with no more than five mice…
CP2K QMMM Calculation seems to randomly crash
Dear all, I have encountered a very disturbing phenomena. My system is fairly simple: One Na+ in water (up to 800 water molecules). I generated the system using tleap. The plan is the following: Since full semi empirical MD with XTB is costly for 801 atoms, I tried to calculate…
Less number of total Species than Genus in 16S data
DADA2’s classifiers might not be able to classify to the species-level, it will keep the taxonomic level ‘higher’ as necessary. Example with some of my fish ASVs: “Eukaryota”,”Chordata”,”Actinopteri”,”Labriformes”,”Labridae”,NA,NA Only family-level classification, no genus/species “Eukaryota”,”Chordata”,”Actinopteri”,”Kurtiformes”,”Apogonidae”,”Apogonichthyoides”,”Apogonichthyoides timorensis” Classified to the species-level So yeah, you might have more genera classified than species if…
ggplot2 – Graph only showing part of the results in R
I am working with data regarding dogs up for adoption in Washington and Oregon and wanted to see if the gender of the dogs has anything to do with their ability to be adopted. dput(dd_clean_df[1:10, ]) top_gender_breeds <- dd_clean_df %>% group_by(contact_state, breed_primary, sex) %>% summarise(count = n()) %>% arrange(contact_state, desc(count))…
Elevated peripheral levels of receptor-interacting protein kinase 1 (RIPK1) and IL-8 as biomarkers of human amyotrophic lateral sclerosis
Ethics statement All the animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals and were approved by the Animal Care Committee of Three Gorges University, China (2019060B). The approval of primidone off-label prescription for ALS patients was obtained from the Ethics Committee…
High-throughput sequencing of Diatoms using V4 region of 18S rRNA gene in Bayug Island, Iligan City, Philippines
Almarez DN, Almarez FJS, Baulete EM. 2014. Bayug Island Aquasilvi Program: An Eco-Governance Strategy for Climate Change Adaptation and Mitigation. J Govern Dev 10(2): 35-54. DOI: 10.32890/jgd. Alongi DM. 2014. Carbon cycling and storage in mangrove forests. Ann Rev Mar Sci 6: 195-219. DOI: 10.1146/annurev-marine-010213-135020. Balint M, Pfenninger M, Grossart…
Combine MAF, GISTIC, and clinical data using maftools
My goal is to produce an OncoPlot with somatic mutation, copy number, and clinical information. I’m able to produce a MAF file with clinical info: maf.plus.clin <- read.maf( maf = maf, clinicalData = clinical.all, ) and also a MAF file with GISTIC info: maf.plus.gistic <- read.maf( maf = maf, gisticAllLesionsFile…
Fibroblast Growth Factor Receptor 2 Inhibitor Market Focuses on Market Share, Size and Projected Forecast Till 2030 | by Isomjohnson | Dec, 2023
What is Fibroblast Growth Factor Receptor 2 Inhibitor? Fibroblast Growth Factor Receptor 2 (FGFR2) inhibitors have emerged as a promising therapeutic class in the field of precision medicine, particularly in the treatment of certain types of cancer. These inhibitors selectively target the FGFR2 signaling pathway, which is responsible for stimulating…
How does mtdna tells about depression?
Open access•Journal Article•DOI Na Cai, Yihan Li, Simon Chang, Jieqin Liang, Chongyun Lin, Xiufei Zhang, Lu Liang, Jingchu Hu, Wharton Chan, Kenneth S. Kendler, Tomas Malinauskas, Guo-Jen Huang, Qibin Li, Richard Mott, Jonathan Flint – Show less +14 more The paper discusses the relationship between mitochondrial DNA (mtDNA) and major…
r – Creating a `ggplot2` plot with distinct length scales for different x axis segments
I want to create a ggplot2 plot with multiple geom_rect objects. For now, I’m trying to create a plot with two geom_rect objects. I need segments of the x axis to be one of two “scales”. For example, the physical length between 0 and ten on the x axis might…
r – Adjust plot.background margin in ggplot
My plot background is much larger than the plot and I can’t figure out how to reduce it. I have hacked a way to bring the title closer to the plot, but there is still a large space below the plot. This causes issues when I use cowplot to place…
Methylation Analysis Tutorial in R_part1
The code and approaches that I share here are those I am using to analyze TCGA methylation data. At the bottom of the page, you can find references used to make this tutorial. If you are coming from a computer background, please bear with a geneticist who tried to code…
CD CHO Media Market Research Report Unlocks Analysis on the Market Financial Status, Market Size, and Market Revenue upto 2030
CD CHO Media Market Analysis and Latest Trends CD CHO Media is a specialized cell culture medium used for the growth and production of CHO (Chinese Hamster Ovary) cells in biopharmaceutical manufacturing processes. CHO cells are widely used in the production of therapeutic proteins, vaccines, and monoclonal antibodies. CD CHO…
pytorch-cluster: FTBFS: ImportError: Failed to load PyTorch C extensions:
Source: pytorch-cluster Version: 1.6.1-2 Severity: serious Justification: FTBFS Tags: trixie sid ftbfs User: lu…@debian.org Usertags: ftbfs-20231212 ftbfs-trixie Hi, During a rebuild of all packages in sid, your package failed to build on amd64. Relevant part (hopefully): > debian/rules clean > dh clean –buildsystem pybuild > dh_auto_clean -O–buildsystem=pybuild > I: pybuild…
How to use RStudio
Article Summary Box Customizing the RStudio Environment significantly boosts productivity, with options like project-specific settings and theme personalization enhancing the user experience. Advanced ggplot2 Techniques in data visualization offer a blend of aesthetics and functionality, enabling the creation of visually striking and informative plots. Efficient Data Management strategies in RStudio,…
r – Having trouble getting stat_pvalue_manual to facet across my ggplot
I’d like to make a facetted plot, with the p-values from my emmeans() table added using stat_pvalue_manual(). However, I can’t seem to get them to facet. Any help would be very welcome! Here is some demo data: demo_data <- structure(list(tissue = structure(c(1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L, 1L,…
Change baseurl for Openapi Resource – Queries and Resources
Hello! I would like to use the Amazon SP Api with Retool. I tried it with the restapi and it works. But it would be easier for me to import the JSON model file with Openapi. However, I cannot change the stored baseurl?Can someone tell me the best way to…
Marstacimab Phase 3 Data Presented at ASH 2023 Demonstrate Significant Bleed Reduction in Hemophilia A and B
Marstacimab reduced annualized bleeding rate by 35% and 92% compared to routine prophylaxis and on-demand treatment in patients with hemophilia A and B without inhibitors, respectively Consistent reduction in bleeding rates observed after an additional 16 months of follow-up in the trial’s long-term extension observational study NEW YORK–(BUSINESS WIRE)– Pfizer…
ggplot2 – “Wrapping” heatmap columns in R using ggplot
I’m working on creating a heatmap of objects in a shelving unit using RStudio. The way the shelving is configured IRL is one column is shelves #1-8 from top to bottom, next column is shelves #9-16 top to bottom, and so on, for 54 total shelving units, repeated for 6…
Bladder Cancer Pipeline, FDA Approvals, Clinical Trials Developments and Companies 2023
PRESS RELEASE Published December 7, 2023 DelveInsight’s, “Bladder Cancer Pipeline Insight 2023” report provides comprehensive insights about 100+ companies and 100+ pipeline drugs in the Bladder cancer pipeline landscape. It covers the Bladder Cancer pipeline drug profiles, including clinical and nonclinical stage products. It also covers the Bladder Cancer pipeline…
Refurbished DNA Sequencing Platforms Market Insights, Market Players and Forecast Till 2030
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absolute running error: Error in if (!is.na(res)) { : the condition has length > 1 0 the following are my R codes: install.packages(“numDeriv”) BiocManager::install(“DNAcopy”) install.packages(“ABSOLUTE_1.0.6.tar.gz”,repos = NULL) library(numDeriv) library(ABSOLUTE) RunAbsolute( seg.dat.fn = “P01_1_GISTIC_segment”, sigma.p = 0, max.sigma.h = 0.2, min.ploidy = 0.5, max.ploidy = 8, primary.disease = “NPC”, platform =…
Gene association analysis of an osteopontin polymorphism and ketosis resistance in dairy cattle
Methods Approval for animal experiments The prof. Dorota Ziyba-Przybylska as a Chairman of ethics review board and Dean of the Faculty of Animal Science, University Agriculture in Krakow considers that this type of project does not fall under the legislation for the protection of animals used for scientific purposes. The…
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Oburger E, Schmidt H. New methods to unravel rhizosphere processes. Trends Plant Sci. 2016;21:243–55. Article CAS PubMed Google Scholar Rout ME, Southworth D. The root microbiome influences scales from molecules to ecosystems: the unseen majority. Am J Bot. 2013;100:1689–91. Article PubMed Google Scholar Pratama AA, Terpstra J, de Oliveria ALM,…
Genomic Workstation Market Dynamics 2023-2030: Also about Its Market Trends, Projections, and Opportunities
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