Tag: NAMESPACE

Running DESeq2 from rpy2 – Askdevz

If you open R and type: you will see that the assay function is not actually coming from DESeq2, but from its dependency which is called SummarizedExperiment: > assay standardGeneric for “assay” defined from package “SummarizedExperiment” function (x, i, withDimnames = TRUE, …) standardGeneric(“assay”) <bytecode: 0x5586a354db90> <environment: 0x5586a3535e20> Methods may…

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Error while installing “object I is not exported by namespace:S4Vectors

met a problem while installing the LTLA/SingleR 1.5.3, urgent, thank you very much for help installing to /home/binf0xx/R/x86_64-pc-linux-gnu-library/4.0/00LOCK-SingleR/00new/SingleR/libs ** R ** inst ** byte-compile and prepare package for lazy loading Error: object ‘I’ is not exported by ‘namespace:S4Vectors’ Execution halted ERROR: lazy loading failed for package ‘SingleR’ removing ‘/home/binf0xx/R/x86_64-pc-linux-gnu-library/4.0/SingleR’ Read…

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NCBI C++ ToolKit: src/objtools/data_loaders/genbank/incr_time.cpp Source File

Go to the documentation of this file. Go to the SVN repository for this file. 44  const string& driver_name, 47  m_InitTime = x_GetDoubleParam(conf, driver_name, params.m_Initial); 48  m_MaxTime = x_GetDoubleParam(conf, driver_name, params.m_Maximal); 49  m_Multiplier = x_GetDoubleParam(conf, driver_name, params.m_Multiplier); 50  m_Increment = x_GetDoubleParam(conf, driver_name, params.m_Increment); 55  const string& driver_name, 68  if (…

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[BioC] rtracklayer 1.6: invalid class “ucscCart” object

Dear Bioc, Following the rtracklayer documentation, section 2.2.4, ‘A Shortcut’, I encounter the following error browseGenome (subTargetTrack) Error in validObject(.Object) :invalid class “ucscCart” object: superclass “ANYTHING” not defined in the environment of the object’s class traceback () 13: stop(msg, ” “, errors, domain = NA)12: validObject(.Object)11: initialize(value, …)10: initialize(value, …)9:…

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Error in SummarizedExperiment

I have installed DESeq2 version 1.36.0 samples <- colnames(txi$counts) group <- as.factor(c(“control”,”control”,”control”,”control”,”control”,”diet”,”diet”,”diet”,”diet”,”diet”, “control”,”control”,”control”,”control”,”control”,”diet”,”diet”,”diet”,”diet”,”diet”,”diet”)) coldata <- data.frame(samples, group, stringsAsFactors = F) coldata <- coldata[,c(“samples”,”group”)] coldata$samples <- factor(coldata$samples) coldata$group <- factor(coldata$group) rownames(coldata) <- sub(“fb”, “”, rownames(coldata)) all(rownames(coldata$samples) %in% colnames(txi)) all(rownames(coldata) == colnames(txi)) TRUE library(DESeq2) ddsTxi <- DESeqDataSetFromTximport(txi, colData = coldata, design =…

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Solved Need some help In C++, thanks!! /*Class

Need some help In C++, thanks!! /*Class Hierarchy Design a Class “Hierarchy” of 4 derived classes representing 4 generations of a family tree as follow:Grandparent -> Parent -> Child -> Grandchild Add a unique “trait” data member of type integer to EACH CLASS in the hierarchy: A, B, C, DNote…

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How to link GCP VM disks allocated by dynamic pvc-HEXHEXHEX and `claim-username` again after irrevocably losing all kubernetes yaml? – Zero to JupyterHub on Kubernetes

TL;DR I destroyed my claim-usernames on a prod cluster deployed using current z2jh, and have a mob of nervous scientists scared they lost their data… how do I go about rebinding a hundred disk-HEXHEX GCP VM disks to the appropriate jupyterhub users? In the process of finally deploying / converting…

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deseq2 problem

deseq2 problem 0 Hi I am trying to draw a PCA plot with DESeq2 but somehow I cannot use DESeq2 functions. It is a really simple code i wil be pasting below. > transform <- DESeq2::rlog(eliminated_data, blind = TRUE) Error in (function (classes, fdef, mtable) : unable to find an…

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GDCprepare of RNAseq counts produces error

GDCprepare of RNAseq counts produces error 1 @76ac7b25 Last seen 12 minutes ago Canada Hello everyone! I have been using the TCGAbiolinks package for the last couple years to access RNAseq data for the TCGA-LAML project. Just very recently, I had noticed that I could no longer use GDCquery to…

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Separate exogenous from endogenous transcripts using Salmon RNAseq DTU

Dear friends, We are trying to use Salmon for DTU analysis. We want to separate exogenous from endogenous transcripts by following this post www.biostars.org/p/443701/ and this paper f1000research.com/articles/7-952 We are focusing on a gene called ASCL1 (endo-ASCL1). We transduced cells with lentiviral vector containing ASCL1 ORF only (Lenti-ASCL1). There should…

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GDCquery_Maf error

GDCquery_Maf error 0 @76e1237b Last seen 1 day ago Singapore Hi all, I really need some help. I am trying to run GDCquery_Maf which worked fine until yesterday. Now I get the following error: Error in GDCquery(paste0(“TCGA-“, tumor), data.category = “Simple Nucleotide Variation”, : Please set a valid workflow.type argument…

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subpopulations available in MafH5.gnomAD.v3.1.1.GRCh38

subpopulations available in MafH5.gnomAD.v3.1.1.GRCh38 1 @b14a6f0d Last seen 16 hours ago United States Are subpopulation MAFs available for gnomADv.3.1.1 with any package, like they are in MafDb.gnomAD.r2.1.hs37d5? I’m trying to use Genomic Scores to obtain all variants in a genomic range with MAF in any subpopulation >= cutoff. I tried…

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Compute shader not working – trying to just make it work at all – OpenGL

HI So, I want to try the compute shader to calculate transfer functions on my 3D texture, and output a new 3D texture, but I cant get it to work at all… Nothing happens. Right now I am just trying to fill the whole new 3D texture (outVolumeTexture) with values…

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Bioconductor installation problems

Dear Friends, I have been having considerable difficulty doing various package related bioconductor actions. Here is my system specifications: ======================================================================= macOS Monterey Version: 12.3 (21E230) MacBook Pro (Retina, 13-inch, Early 2015) Memory: 16 GB 1867 MHz DDR3 RStudio 2021.09.0 Build 351 R version 4.1.2 (2021-11-01) — “Bird Hippie” Copyright (C)…

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Running rstudio (but not R alone) inside of a conda environment complains about finding Rccp.so

I find that when I try to load some packages in a conda environement, when using rstudio (but not when I’m using R directly), I get an error message about a missing Rcpp.so file. I activate my conda environment (which is running R version 4.1), open RStudio (which I installed…

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AnnotationHub installation ‘Rcpp’ 1.0.6 is already loaded, but >= 1.0.7 is required

Hello, I am currently trying to install AnnotationHub but I am running into problems. I use R 4.0.4, renv (version 0.15.4) and BiocVersion 3.12. When I run BiocManager::install(“AnnotationHub”), I get the following error: Warning message: This project is configured to use R version ‘4.1.1’, but ‘4.0.4’ is currently being used….

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Bioconductor Package Installation

When I try to install the gtf for hg38 BiocManager::install(“TxDb.Hsapiens.UCSC.hg38.knownGene”) I get the following error: ‘getOption(“repos”)’ replaces Bioconductor standard repositories, see ‘?repositories’ for details replacement repositories: CRAN: cran.rstudio.com/ Bioconductor version 3.14 (BiocManager 1.30.16), R 4.1.2 (2021-11-01) Installing package(s) ‘TxDb.Hsapiens.UCSC.hg38.knownGene’ Error in readRDS(dest) : error reading from connection Per stackoverflow.com/questions/67455984/getoptionrepos-replaces-bioconductor-standard-repositories-see-reposito I…

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Pathway analysis of RNAseq data using goseq package

Hello, I have finished the RNA seq analysis and I am trying to perform some pathway analysis. I have used the gage package and I was looking online about another package called goseq that takes into account length bias. However, when I run the code I get an error. How…

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CORS issues behind nginx ingress after updating to JupyterHub 2.1.1 – JupyterHub

Hi there, I noticed multiple issues after upgrading to JupyterHub 2.1.1 from 2.0.0. I stopped seeing spawn progress when starting the singleuser server. Logs: [I 2022-03-04 20:57:47.436 JupyterHub log:189] 302 GET /hub/spawn -> /hub/spawn-pending/konstantin.taletskiy@labshare.org (konstantin.taletskiy@labshare.org@192.168.15.108) 1006.02ms [I 2022-03-04 20:57:47.524 JupyterHub pages:405] konstantin.taletskiy@labshare.org is pending spawn [I 2022-03-04 20:57:47.528 JupyterHub log:189]…

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CHECK report for GGBase on malbec1

BioC 3.4: CHECK report for GGBase on malbec1 This page was generated on 2017-04-15 16:09:39 -0400 (Sat, 15 Apr 2017). GGBase 3.36.0VJ Carey Snapshot Date: 2017-04-14 17:17:13 -0400 (Fri, 14 Apr 2017) URL: hedgehog.fhcrc.org/bioconductor/branches/RELEASE_3_4/madman/Rpacks/GGBase Last Changed Rev: 122710 / Revision: 128728 Last Changed Date: 2016-10-17 14:45:06 -0400 (Mon, 17 Oct 2016) malbec1  Linux (Ubuntu 16.04.1 LTS) / x86_64   OK   OK  [ WARNINGS ] tokay1  Windows Server 2012 R2 Standard / x64   OK   OK   WARNINGS   OK  morelia  Mac OS X Mavericks (10.9.5) / x86_64   OK   OK   WARNINGS   OK  Summary Package: GGBase Version: 3.36.0…

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DESeq2 and high prefiltering cutoff

DESeq2 and high prefiltering cutoff 1 @255004b1 Last seen 3 hours ago United States Hi, I am curious about prefiltering with DESeq2. I understand from this site and reading the DESeq2 vignette that prefiletering is really unnecessary as DESeq2 has a stringent filtering that it does. However, I’m seeing better…

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r – ggplot2 running for minutes without plotting

I am attempting to plot the below vector, but when I run the function, it just continues to run and does not plot. I have waited 5 minutes before I feel uncomfortable and click stop in the console. Wondering what is going on. Up until this point I have had…

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Gromacs: gmx::analysismodules::anonymous_namespace{rdf.cpp}::Rdf Class Reference

Public Member Functions virtual void  initOptions (IOptionsContainer *options, TrajectoryAnalysisSettings *settings)   Initializes options understood by the module. More…   virtual void  optionsFinished (TrajectoryAnalysisSettings *settings)   Called after all option values have been set. More…   virtual void  initAnalysis (const TrajectoryAnalysisSettings &settings, const TopologyInformation &top)   Initializes the analysis. More…  …

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php-openapi make implementations of built-in interfaces PHP 8.1 friendly

Problem Unfortunately, PHP 8.1 throws Fatal Error if the built-in interface implementation is incompatible with the new, type enhanced interfaces. Example error: Fatal error: During inheritance of ArrayAccess: Uncaught Return type of cebeopenapispecResponses::offsetExists($offset) should either be compatible with ArrayAccess::offsetExists(mixed $offset): bool, or the #[ReturnTypeWillChange] attribute should be used to temporarily…

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traviz 1.0.0 installation fails: ERROR: lazy loading failed

Hi, I cannot install traviz package (version 1.0.0) from Bioconductor on a linux machine (from source). I have a conda environment, and I installed traviz from conda, but it cannot be used – when I do library(traviz) R just crashes and quits without any message. So I tried to install…

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boost/type_traits/integral_constant.hpp – 1.78.0

boost/type_traits/integral_constant.hpp // (C) Copyright John Maddock 2015. // Use, modification and distribution are subject to the // Boost Software License, Version 1.0. (See accompanying file // LICENSE_1_0.txt or copy at www.boost.org/LICENSE_1_0.txt) #ifndef BOOST_TYPE_TRAITS_INTEGRAL_CONSTANT_HPP #define BOOST_TYPE_TRAITS_INTEGRAL_CONSTANT_HPP #include <boost/config.hpp> #include <boost/detail/workaround.hpp> #if (BOOST_WORKAROUND(BOOST_MSVC, BOOST_TESTED_AT(1400)) || BOOST_WORKAROUND(BOOST_BORLANDC, BOOST_TESTED_AT(0x610)) || BOOST_WORKAROUND(__DMC__, BOOST_TESTED_AT(0x840)) || BOOST_WORKAROUND(__MWERKS__,…

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identical(current_classes, .UCSC_TXCOL2CLASS) is not TRUE

GenomicFeatures::makeTxDbFromUCSC failing with an error: identical(current_classes, .UCSC_TXCOL2CLASS) is not TRUE 1 @mikhail-dozmorov-23744 Last seen 1 day ago United States Hi,The GenomicFeatures::makeTxDbFromUCSC function fails with: library(GenomicFeatures) > hg19.refseq.db <- makeTxDbFromUCSC(genome=”hg19″, table=”refGene”) Download the refGene table … Error in .fetch_UCSC_txtable(genome(session), tablename, transcript_ids = transcript_ids) : identical(current_classes, .UCSC_TXCOL2CLASS) is not TRUE OK The…

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R ggtree: How to label single tree tip with ggtree similar to labeling nodes with geom_cladelabel

I’m having trouble with labeling single tips in my tree with ggtree. I’m trying to highlight and label nodes from a tree with geom_hilight and geom_cladelabel. This seems to work fine with nodes that have more than 1 tree tip, but when I try to label a single tip, I…

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Delightful code generation for OpenAPI specs for Swift written in Swift

Delightful code generation for OpenAPI specs for Swift written in Swift. Fast: processes specs with 100K lines of YAML in less than a second Smart: generates Swift code that looks like it’s written by hand Reliable: tested on 500K lines of publically available OpenAPI specs producing correct code every time…

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r – RSQLite Error “hash is not an exported object”

I am trying to scrape some pitchf/x data and store it in an SQLite database. However, I am receiving the following error when I run the following code: library(RSQLite) library(dplyr) db <- src_sqlite(“pitchfx.sqlite3”, create = T) This is the error: Error in h(simpleError(msg, call)) : error in evaluating the argument…

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[R] FinCal and ggplot2 packages in r could not be loaded.

Hello R users community, I installed ‘FinCal’ and ‘ggplot2’ packages in r and tried to load them, but I couldn’t load them. R gave me the following error. >library(FinCal) Error: package or namespace load failed for ‘FinCal’ in loadNamespace(j <- i[[1L]], c(lib.loc, .libPaths()), versionCheck = vI[[j]]): there is no package…

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docker – SLURM cluster inside k8s cannot run srun command

I’m a beginner k8s user, I’m trying to recreate this docker-compose SLURM cluster with kubernetes. First I converted the docker-compose.yaml file into k8s yaml file in order to use kubectl apply -f . to create pods and services. I’m using minikube on my computer with the none driver (like this…

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Analyzing RNA-seq data with DESeq2 Tutorial

I am working through the Analyzing RNA-seq data with DESeq2 tutorial, and running it through RMarkdown. Loading the tximeta package in the tutorial code below: coldata <- samples coldata$files <- files coldata$names <- coldata$run library(“tximeta”) # se <- tximeta(coldata) # ddsTxi <- DESeqDataSet(se, design = ~ condition) Is causing the…

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PyTorch model conversion – Technical Support

I’m unable to convert a model in pytorch This is the script to download the pretrained weights import torch import torchvision.models as models resnet18 = models.resnet18(pretrained=True) torch.save(resnet18, ‘resnet18.pt’) This is the script for converting ./convert –model-name resnet18 –platform pytorch –model resnet18.pt –input-size-list ‘3,224,224’ –mean-values ‘103.94,116.78,123.68,58.82’ –quantized-dtype asymmetric_affine –kboard VIM3 –print-level…

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Using special characters in Rstudio

Using special characters in Rstudio I am working with some special characters in Rstudio. It coverts them into plain letters. print(“Safarzyńska2013”) [1] “Safarzynska2013” x <- “Māori” x [1] “Maori” Is there any way to read in the exact original characters. Following info might be helpful: Rstudio default encoding is UTF-8…

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Design formula in DESeq2

Hello, I am using DESeq2 for analysis of RNAseq data. I would like to ask you about the design in the DESEq2 formula. I have tissue from animals treated with a chemical and my animal model is a colorectal cancer model. My variables are gender (male or female), treatment (treated…

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main-arm64-default][devel/RStudio] Failed for RStudio-2021.09.1+372 in build

You are receiving this mail as a port that you maintain is failing to build on the FreeBSD package build server. Please investigate the failure and submit a PR to fix build. Maintainer: y…@freebsd.org Log URL: ampere2.nyi.freebsd.org/data/main-arm64-default/p7539e33f88ff_s169b368a62/logs/RStudio-2021.09.1+372.log Build URL: ampere2.nyi.freebsd.org/build.html?mastername=main-arm64-default&build=p7539e33f88ff_s169b368a62 Log: =>> Building devel/RStudio build started at Wed Dec 8…

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Gromacs: gmx::ISerializer Class Reference

#include <gromacs/utility/iserializer.h> Inherited by anonymous_namespace{keyvaluetreeserializer.cpp}::RefDataSerializer, gmx::FileIOXdrSerializer, gmx::InMemoryDeserializer, and gmx::InMemorySerializer. Interface for types that convert standard data types into a form suitable for storage or transfer. Different implementations could suit MPI, file I/O, or in-memory conversion. virtual bool  reading () const =0   Returns whether the serializer is reading or writing,…

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Gromacs: Class Members – Variables

Gromacs: Class Members – Variables   – n – N : t_UmbrellaWindow , t_bb n : t_coordselection , t_methoddata_kwreal , t_methoddata_permute , t_partition , gmx_fft_fftpack , t_spheresurfacebin , t_methoddata_kwint n_alloc : t_spheresurfacebin n_at_lam : df_history_t n_dev : gmx_gpu_info_t n_dev_compatible : gmx_gpu_info_t nalloc : cu_atomdata , cl_atomdata , gmx_ana_selvalue_t , swap_compartment…

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Finding the significance of the overlap between 2 or more gene sets using simulation in R.

TLDR: Example R function to calculate significance of overlap of 2 or more gene sets. genes_all is a vector that contains all genes, and gene_sets takes a list of vectors for each gene set. I encourage people to read the full tutorial and attempt to reproduce the code themselves (especially…

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Outliers on DESEq2 Results

I have an RNAseq dataset, where one of the genes I intend to analyze has hundreds of counts ranging from 10 to 12, with a few counts > 9000. I process this data in Deseq2 and get that the gene is differentially expressed across several samples of interest. What can…

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‘package ‘limma’ could not be loaded’

trinity run_DE_analysis.pl Failed with error: ‘package ‘limma’ could not be loaded’ 1 Hi Experts, can someone please help me with this error while running run_DE_analysis.pl for DESeq2 analysis Loading required package: edgeR Loading required package: limma Error: package or namespace load failed for ‘limma’: .onLoad failed in loadNamespace() for ‘limma’,…

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Gromacs: Namespace Members

Here is a list of all documented namespace members with links to the namespaces they belong to: – d – decideWhetherToUseGpusForBonded() : gmx decideWhetherToUseGpusForNonbonded() : gmx decideWhetherToUseGpusForNonbondedWithThreadMpi() : gmx decideWhetherToUseGpusForPme() : gmx decideWhetherToUseGpusForPmeWithThreadMpi() : gmx DefaultRandomEngine : gmx detectGroup() : gmx Distribution : gmx do_lincs() : gmx do_lincsp() : gmx…

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Gromacs: gmx::internal Namespace Reference

Internal GROMACS namespace. This namespace is used to contain some implementation-specific functions and classes. These are not meant for direct user access, but typically reside in public headers because of implementation reasons. template<typename T > static void  ignoreValueHelper (const T &)   Helper for ignoring values in macros. More…  …

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Bioconductor Code: Browse

 About browsing: master Branches RELEASE_3_13 master Files Commits Stats Network Graph hca Clone ZIP TAR × SSH HTTPS Name Mode Size R 040000 man 040000 tests 040000 vignettes 040000 .Rbuildignore 100644 0 kb .gitignore 100644 1 kb DESCRIPTION 100644 1 kb LICENSE 100644 0 kb LICENSE.md 100644 1 kb NAMESPACE…

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Mapping unique GO term description given a specific GO id

Mapping unique GO term description given a specific GO id 0 I have a list of GO ids and I want to find unique term description such that if I provide say 200 GO IDs I will give 200 specific GO terms. The code snippet I am using is given…

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hjust in geom_cladelab not working properly

hjust in geom_cladelab not working properly 0 I have an issue with hjust of clade lab when using geom_cladelab. The justification does not change when specifying the labels to align with the outer circle and having angle = “auto” . I have added an reprex of the issue. The problem…

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spots not filling the whole tissue image

Issue with Seurat SpatialPlot: spots not filling the whole tissue image 0 In Seurat, SpatialPlot generates a plot with an enlarged/expanded image of tissue section as compared to the original spot image. This seems to happen on the relatively small image with a number of spots around 500. I ‘d…

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GROMACS: src/gromacs/ewald/pme_pp.h | Fossies

GROMACS: src/gromacs/ewald/pme_pp.h | Fossies “Fossies” – the Fresh Open Source Software Archive Member “gromacs-2021.3/src/gromacs/ewald/pme_pp.h” (18 Aug 2021, 5141 Bytes) of package /linux/privat/gromacs-2021.3.tar.gz: As a special service “Fossies” has tried to format the requested source page into HTML format using (guessed) C and C++ source code syntax highlighting (style: standard) with…

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GROMACS: src/gromacs/utility/any.h | Fossies

GROMACS: src/gromacs/utility/any.h | Fossies “Fossies” – the Fresh Open Source Software Archive Member “gromacs-2021.3/src/gromacs/utility/any.h” (18 Aug 2021, 8080 Bytes) of package /linux/privat/gromacs-2021.3.tar.gz: As a special service “Fossies” has tried to format the requested source page into HTML format using (guessed) C and C++ source code syntax highlighting (style: standard) with…

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weird MAplot or volcano plot of DESeq2 diff result

Hi, every one. I find a werid MAplot or volcano plot of DESeq reuslt. I am wondering whether you can give me some advice. This diff result is from two cell type bulk RNA-seq. I use two specific marker to get these two cell type using Flow cytometer. I alreadly…

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