Tag: NextDenovo

Sample collection Captive breeding individuals of H. coronatus (Mantodea, Hymenopodidae) hatched from the same ootheca that was collected from the Xishuangbanna rainforest, Yunnan Province, China in 2018. Individuals of D. lobata (Mantodea, Deroplatyidae) were collected from a captive breeding center in Beijing, China in 2018. All individuals were housed in semitransparent…

Formenti, G. et al. The era of reference genomes in conservation genomics. Trends Ecol. Evol. 37, 197–202 (2022). Article  CAS  PubMed  Google Scholar  Lee, H. et al. Third-generation sequencing and the future of genomics. bioRxiv, 048603 (2016). Irestedt, M. et al. A guide to avian museomics: Insights gained from resequencing…

NEW YORK – Researchers at the University of California, Riverside, and the Agricultural Genomics Institute at Shenzhen, China, have assessed the most popular assembly tools for high-fidelity data from Pacific Biosciences long-read sequencers, testing them on data for eukaryotic genomes and metagenomic datasets, both real and synthetic. They concluded that…

Ethics statement All animal experimental procedures were approved by the Biomedical Ethics Committee of Qufu Normal University (approval number: 2022001). Sampling and sequening The experimental sample is a wounded male duck found during the wild bird survey in Jiangsu, China, which died unexpectedly during rescue. We dissected the sample and…

Ethics statement All animal handling and experimental procedures were approved by the Qufu Normal University Biomedical Ethics Committee (approval number: 2022001). Sample and sequencing Baer’s pochard tissue for whole-genome sequencing was obtained from a dead individual that had strayed into a fishing net in Shandong (China). The muscle tissue that…

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MissAssembly Correction 0 I am working on fungal genome assembly using long reads and I am getting lots of misassemblies. Assembly was made using NextDenovo, which made 11 contigs with lots of misassemblies. stats are as follows: Largest contig = 8126790 N50 = 5981938 misassemblies = 1939 I applied nextPolish…

Problem with NextDenovo fungal assembly 1 Hi guys, I am new in Genome assembly, I have fungus genome sequencing data from Oxford Nano Tech with 200 X coverage. I used NextDenovo for assembly. I am using my personal system with 32 GB RAM. The parameter file I set is like…

How to get the assembly larger than 3.5Gb in NextDenovo? 0 Hey there! NextDenovo assembles only 3.5Gb. Is it possible to somehow make the NextDenovo go to 17Gb? Thanks, Ural assemble NextDenovo genome large • 42 views Source link