Tag: oocyte

Difficulty Difficult 52 Page 101 315 Art Question 5006 As depicted in the figure

317.Art Question 5.008 The figure below depicts a Xenopus oocyte with hundreds of nucleoli.Why would an oocyte need so many nucleoli when most cells have only one or two? Ans:Oocytes, after they mature into eggs, will be fertilized and will start making large amounts of protein.They will not be able…

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Development of an mRNA electroporation method in immature mouse oocytes to visualize protein dynamics during early development

Abstract One of the major cause of oocyte quality deterioration along with aging, chromosome segregation abnormalities occur mainly during meiosis I. However, currently, there is a technical limitation in the introduction of mRNA into premature oocytes without impairing embryonic developmental ability. In this study, we established a low-invasive electroporation (EP)…

Continue Reading Development of an mRNA electroporation method in immature mouse oocytes to visualize protein dynamics during early development

The effect of Coenzyme Q10 on mitochondrial biogenesis in mouse ovarian follicles during in vitro culture

doi: 10.1017/S0967199423000461. Online ahead of print. Affiliations Expand Affiliations 1 School of Biology, Damghan University, Damghan, Iran. 2 Institute of Biological Sciences, Damghan University, Damghan, Iran. Item in Clipboard Roya Harsini et al. Zygote. 2023. Show details Display options Display options Format AbstractPubMedPMID doi: 10.1017/S0967199423000461. Online ahead of print. Affiliations 1…

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Wall Street eyes CRISPR Therapeutics’ growth By Investing.com

© Reuters. Explore Wall Street’s expert insights with this ProResearch article, which will exclusively be available to InvestingPro subscribers soon. Enhance your investment strategy with ProPicks, our newest product featuring strategies that have outperformed the S&P 500 by up to 700%. This Cyber Monday, enjoy up to 60% off, plus…

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Postdoctoral Researcher in Epigenetics and Bioinformatics, Czech

Postdoctoral Researcher in Epigenetics and Bioinformatics: The newly established Developmental Epigenetics and Bioinformatics Laboratory at the University of South Bohemia in Ceske Budejovice, Czech Republic, is seeking an enthusiastic postdoc to explore the secrets of long-term oocyte and ovarian quality in long-lived African mole-rats and other non-model rodents. The position…

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Postdoc for the Developmental Epigenetics and Bioinformatics Laboratory

Přehled Newly established Developmental Epigenetics and Bioinformatics Laboratory is looking for an enthusiastic postdoc that is not afraid of challenges associated with work with non-model mammals. Where: University of South Bohemia, Ceske Budejovice, Czech Republic (international campus, friendly atmosphere, Alps not too far, town of ~100 000 inhabitants, good for…

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CYIL vol. 9 (2018) – Page 304

MARTIN ŠOLC CYIL 9 ȍ2018Ȏ In case of mtDNA, however, the situation is rather unique: mitochondria can be considered part of the germline only in oocytes, and not in sperm. In other words, MRT in female embryos arguably represents a germline modification which is transmissible to the next generation, while…

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Genome haplarithmisis sheds light on complex genetic landscape of miscarriages

About 10-15% of pregnancies fail after conception has been recognized, amounting to 23 million losses a year. Chromosomal anomalies underlie many embryonic and fetal losses, but their exact frequency and localization to the embryo or placenta are still unclear. A new study published in Nature Medicine reports on a chromosomal…

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Quantitative analysis of mitochondrial DNA in porcine-mouse cloned embryos

. 2023 Jul;65(4):767-778. doi: 10.5187/jast.2023.e2. Epub 2023 Jul 30. Affiliations Expand Affiliation 1 Division of Animal & Dairy Science, Chungnam National University, Daejeon 34134, Korea. Item in Clipboard Hyeonyeong Shin et al. J Anim Sci Technol. 2023 Jul. Show details Display options Display options Format AbstractPubMedPMID . 2023 Jul;65(4):767-778. doi: 10.5187/jast.2023.e2….

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Science news in review: Nov. 5

As we enter the November stretch of the semester, researchers around the world are also working hard to advance efforts that can be beneficial to human health. This week’s science news highlights endeavors to understand various health challenges and identify treatments to combat them.  New drug for treating antimicrobial-resistant gonorrhea…

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Bioinformatics analysis and experimental validation of tumorigenic role of PPIA in gastric cancer

Expression of PPIA in pan-cancer and prognostic value of PPIA in GC To assess the effects of PPIA on the genesis of human tumor, TCGA database was utilized to detect the mRNA levels of PPIA in 33 types of cancer. The findings demonstrated that the levels of PPIA were upregulated…

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Domain definition and preliminary functional exploration of the endonuclease NOBP-1 in Strongyloides stercoralis | Parasites & Vectors

Boes J, Slotved HC, Murrell KD, Eriksen L, Roepstorff A, Nansen P, et al. Alternative migration routes of Ascaris suum in the pig. J Parasitol. 2002;88:180–3. Article  CAS  PubMed  Google Scholar  Nikolaou S, Gasser RB. Prospects for exploring molecular developmental processes in Haemonchus contortus. Int J Parasitol. 2006;36:859–68. Article  CAS …

Continue Reading Domain definition and preliminary functional exploration of the endonuclease NOBP-1 in Strongyloides stercoralis | Parasites & Vectors

MICA: a multi-omics method to predict gene regulatory networks in early human embryos

Introduction After the fusion of the oocyte and sperm, the zygote undergoes a series of cell divisions until it forms a blastocyst before implantation into the uterus. A human blastocyst is formed of a fluid-filled cavity and ∼200 cells that comprise three distinct cell types: the trophectoderm (TE), which gives…

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Identification of CCZ1 as an essential lysosomal trafficking regulator in Marburg and Ebola virus infections

Cells and viruses Haploid mSCs AN3–12 are a feeder independent clonal derivative of HMSc2 isolated from mice oocyte and maintained at IMBA52. The AN3–12 library and knocked out cells used for the haploid screening was obtained from IMBA (Austria)14,52. AN3–12 cells were validated by STR analysis. Haploid mES cells were…

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Toxins | Free Full-Text | Smart-seq2 Technology Reveals a Novel Mechanism That Zearalenone Inhibits the In Vitro Maturation of Ovine Oocytes by Influencing TNFAIP6 Expression

Figure 1. ZEN concentration screening and transcriptome data validation. (A) The growth status of sheep oocytes cultured in vitro after 36 h of exposure to different concentrations of ZEN(CK,10 μM, 20 μM, and 30 μM) (10×, 100 μm), (B) qRT-PCR results of SOD1, CCNB1, GPX1, CAT, CDK1, and SOD2 in…

Continue Reading Toxins | Free Full-Text | Smart-seq2 Technology Reveals a Novel Mechanism That Zearalenone Inhibits the In Vitro Maturation of Ovine Oocytes by Influencing TNFAIP6 Expression

Plastid-localized xanthorhodopsin increases diatom biomass and ecosystem productivity in iron-limited surface oceans

Protein sequence and prediction analysis The amino acid sequence of FcR was deduced from the F. cylindrus genome sequence33. Protein alignments with characterized microbial rhodopsins were performed using the MUSCLE algorithm implemented in Geneious (v.5.6)69. Predictions of subcellular location were performed with SignalP v.4.0 and TargetP (v.1.1)70. Putative transmembrane domains…

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Significant decrease of maternal mitochondria carryover using optimized spindle-chromosomal complex transfer

. 2023 Oct 5;21(10):e3002313. doi: 10.1371/journal.pbio.3002313. eCollection 2023 Oct. Xiaoyu Liao  1 , Wenzhi Li  1 , Kaibo Lin  1 , Wei Jin  1 , Shaozhen Zhang  1 , Yao Wang  1 , Meng Ma  1 , Yating Xie  1 , Weina Yu  1 , Zhiguang Yan  1 , Hongyuan Gao  1 , Leiwen Zhao  1 , Jiqiang Si  1 , Yun Wang …

Continue Reading Significant decrease of maternal mitochondria carryover using optimized spindle-chromosomal complex transfer

Assisted Reproduction Changes Prevent Mitochondrial Diseases

Credit: Lacheev/Getty Images Success rates for reducing the inheritance of mitochondrial diseases using assisted reproduction could be vastly improved through the application of three key changes, research indicates. Using a finer micropipette and a more precise method significantly reduced the transfer of cytoplasm, potentially containing affected mitochondria in mouse and…

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The effect of vitrification on blastocyst mitochondrial DNA dynamics and gene expression profiles

Bosch E, De Vos M, Humaidan P. The future of cryopreservation in assisted reproductive technologies. Front Endocrinol (Lausanne). 2020;11:1–15. Article  Google Scholar  De Geyter C, Calhaz-Jorge C, Kupka MS, Wyns C, Mocanu E, Motrenko T, et al. ART in Europe, 2015: results generated from European registries by ESHRE†. Hum Reprod…

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Optimized protocol to prevent disease transmission through maternal mitochondrial DNA carryover

Several human diseases arise due to defects in mitochondrial DNA (mtDNA), which is distinct from nuclear DNA and is inherited only from maternal ancestors. The prevention of these diseases by mitochondrial replacement (MR) therapy involves the risk of increasing mtDNA mutations, which can subsequently lead to mitochondrial genetic drift. A…

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solving the transplant shortage crisis

On January 7, 2022, a surgery was performed on David Bennett to give him a new heart. But this was no ordinary transplant surgery. Instead of receiving a heart from a human donor, the 57-year-old, who did not qualify for a traditional transplant, received a pig heart. This surgery marked…

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The Role of Mitochondrial DNA in Human Reproduction

This article explores new research that confirms the scientific understanding that mitochondrial DNA (mtDNA) is exclusively inherited from the mother. The study, published in the journal Nature Genetics, was a collaboration among Oregon Health & Science University and other institutions. Previously, it was believed that paternal mtDNA was eliminated after…

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Single-Cell Study Unveils Heterogeneity in Mouse Follicles

【Summary】  A research group led by Professor Kazuki Kurimoto and Assistant Professor Hiroki Ikeda from the Department of Embryology at Nara Medical University has developed a highly sensitive method for quantitative single-cell transcriptome analysis, which enables the precise examination of cells extracted from tissue sections using laser capture microdissection (LCM),…

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Study reveals mature sperm lack intact mitochondrial DNA

New research sheds light on the fundamental scientific premise that mitochondrial DNA — the particular genetic code stored in the organelle that serves as the powerplant for every cell in the body — is exclusively passed down by the mother. The study, a collaboration among Oregon Health & Science University…

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Mature sperm lack intact mitochondrial DNA, study finds

Shoukhrat Mitalipov, Ph.D., director of the Center for Embryonic Cell and Gene Therapy at OHSU, and professor of obstetrics and gynecology, molecular and cellular biosciences, OHSU School of Medicine, OHSU Oregon National Primate Research Center. (OHSU/Christine Torres Hicks) New research provides insight about the bedrock scientific principle that mitochondrial DNA…

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New data on the safety of ‘three genetic parents’ therapy

2023-09-18 17:00:05 An investigation that is published in the magazine ‘Nature Genetics‘ provides information on the fundamental scientific principle that mitochondrial DNA (the distinctive genetic code embedded in the organelle that serves as the powerhouse of each cell in the body) is transmitted exclusively by the mother. The information is…

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Research Reveals Mature Sperm Lacks Intact Mitochondrial DNA

New research provides insight about the bedrock scientific principle that mitochondrial DNA — the distinct genetic code embedded in the organelle that serves as the powerplant of every cell in the body — is exclusively passed down by the mother. The study, a collaboration among Oregon Health & Science University…

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SMAD4 promotes somatic-germline contact during oocyte growth

Abstract Development of the oocyte requires physical contact with the surrounding granulosa cells of the follicle, which provide it with essential nutrients and regulatory signals. This contact is achieved through specialized filopodia, termed transzonal projections (TZPs), that extend from the granulosa cells to the oocyte surface. Transforming growth factor (TGFβ)…

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TimeTalk uses single-cell RNA-seq datasets to decipher cell-cell communication during early embryo development

Curation of early-embryo development single-cell RNA-seq data sets for studying cell-cell communication To identify and study eLRs, we collected public early embryo development scRNA-seq datasets from the mouse MII-oocyte stage to the late blastocyst stage to ensure that scRNA-seq datasets represented every stage of early embryo development. In addition, to…

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The Transformative, Alarming Power of Gene Editing

Mitalipov is an undaunted advocate for heritable editing in humans. A hundred million people suffer from rare genetic diseases, and he believes that somatic editing can do only so much to help them. “It’s not going to alleviate transmission to the next generation,” he said. “The diseases will keep recurring…

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KEGG T07554: 122421214

Entry 122453084         CDS       T07554                                  Name (RefSeq) 14-3-3 protein zeta/delta-like   KO K16197   14-3-3 protein beta/theta/zeta Organism ccad  Cervus canadensis (wapiti) Pathway ccad04110   Cell cycle ccad04114   Oocyte meiosis ccad04151   PI3K-Akt signaling pathway ccad04390   Hippo signaling pathway ccad05160   Hepatitis C ccad05161   Hepatitis B ccad05203   Viral carcinogenesis Brite KEGG Orthology (KO) [BR:ccad00001] 09130 Environmental Information Processing  09132 Signal transduction   04390…

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Maui Fires: How Mitochondrial DNA Will Identify Human Remains

The Maui firestorm was so vast and fast that most identification of human remains will come from bits of persisting DNA from mitochondria. The “Powerhouse of the Cell” Most people likely last encountered mitochondria in high school biology class. The footprint-shaped “powerhouse of the cell” releases energy from breaking the…

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Spatial transcriptomics: recent developments and insights in respiratory research | Military Medical Research

Zepp JA, Morrisey EE. Cellular crosstalk in the development and regeneration of the respiratory system. Nat Rev Mol Cell Biol. 2019;20(9):551–66. Article  CAS  PubMed  PubMed Central  Google Scholar  Kiley JP. Advancing respiratory research. Chest. 2011;140(2):497–501. Article  PubMed  PubMed Central  Google Scholar  Goldstraw P, Ball D, Jett JR, Le Chevalier T,…

Continue Reading Spatial transcriptomics: recent developments and insights in respiratory research | Military Medical Research

Reassessing Stephanofilaria stilesi dermatitis in cattle, with characterization of molecular markers for confirming diagnosis | Parasites & Vectors

Although a common dermatitis of cattle in North America, much remains undescribed about the histological features of S. stilesi, and very little is known about aspects of its evolutionary biology and taxonomic classification. This can be partly attributed to the dated literature on stephanofilariasis in North American cattle [17]. However,…

Continue Reading Reassessing Stephanofilaria stilesi dermatitis in cattle, with characterization of molecular markers for confirming diagnosis | Parasites & Vectors

Optimized bisulfite sequencing analysis reveals the lack of 5-methylcytosine in mammalian mitochondrial DNA

doi: 10.1186/s12864-023-09541-9. Affiliations Expand Affiliations 1 State Key Laboratory of Molecular Biology, Shanghai Institute of Biochemistry and Cell Biology, Center for Excellence in Molecular Cell Science, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, 200031, China. shaozhenyu2017@sibcb.ac.cn. 2 Shanghai Key Laboratory of Medical Epigenetics, Institutes of Biomedical…

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Dynamic RNA profiles in the small intestinal epithelia of cats after Toxoplasma gondii infection | Infectious Diseases of Poverty

Jacobs L. Toxoplasma and toxoplasmosis. Annu Rev Microbiol. 1963;17:429–50. Article  CAS  PubMed  Google Scholar  Montoya JG, Liesenfeld O. Toxoplasmosis. The Lancet. 2004;363(9425):1965–76. Article  CAS  Google Scholar  Dubey JP. Duration of immunity to shedding of Toxoplasma gondii oocysts by cats. J Parasitol. 1995;81(3):410–5. Article  CAS  PubMed  Google Scholar  Dubey JP, Frenkel…

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Genes | Free Full-Text | The Ratio of cf-mtDNA vs. cf-nDNA in the Follicular Fluid of Women Undergoing IVF Is Positively Correlated with Age

Genes 2023, 14(7), 1504; doi.org/10.3390/genes14071504 (registering DOI) Genes 2023, 14(7), 1504; doi.org/10.3390/genes14071504 (registering DOI) Received: 30 June 2023 / Revised: 19 July 2023 / Accepted: 21 July 2023 / Published: 23 July 2023 Round 1 Reviewer 1 Report In this report, the authors presented a study of the cf-mtDNA / cf-nDNA ratio,…

Continue Reading Genes | Free Full-Text | The Ratio of cf-mtDNA vs. cf-nDNA in the Follicular Fluid of Women Undergoing IVF Is Positively Correlated with Age

H3K9me3 signals significantly increase as oocytes become more proximal.

A. Wild-type, met-2 mutant, and set-25 mutant gonads were dissected, fixed, and stained for DNA (blue) and H3K9me3 (red). In N2s, an increase in H3K9me3 signal is observed at the -3 to -1 positions in comparison to more distal oocytes and pachytene nuclei from the same animal. Loss of MET-2…

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A Fertilized Egg Cell Hits “Reset” To Enable Embryo Development

Register for free to listen to this article Thank you. Listen to this article using the player above. ✖ Want to listen to this article for FREE? Complete the form below to unlock access to ALL audio articles. New work by scientists in the U.S. and China…

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WD repeat domain 82 (Wdr82) facilitates mouse iPSCs generation by interfering mitochondrial oxidative phosphorylation and glycolysis

Austenaa LMI, Barozzi I, Simonatto M, Masella S, Chiara GD, Ghisletti S, Curina A, Wit ED, Bouwman BAM, Pretis SD, Piccolo V, Termanini A, Prosperini E, Pelizzola M, Laat WD, Natoli G (2015) Transcription of mammalian cis-regulatory elements is restrained by actively enforced early termination. Mol Cell 60(3):460–474 Article  CAS …

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Thermo Launches New NGS Assays for Preimplantation Testing Research

Credit: Wong Yu Liang/Fotolia Thermo Fisher Scientific this week launched two new next-generation sequencing-based research tools for preimplantation genetic testing-aneuploidy (PGT-A). This type of testing is used for in vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI). The Ion ReproSeq PGT-A Kit and the Ion AmpliSeq Polyploidy Kit are the first…

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Novel economical, accurate, sensitive, single-cell analytical method for mitochondrial DNA quantification in mtDNA mutation carriers

Silva-Pinheiro P, Minczuk M. The potential of mitochondrial genome engineering. Nat Rev Genet. 2022;23(4):199–214. Article  CAS  PubMed  Google Scholar  Davis RL, Wong SL, Carling PJ, Payne T, Sue CM, Bandmann O. Serum FGF-21, GDF-15, and blood mtDNA copy number are not biomarkers of Parkinson disease. Neurol Clin Pract. 2020;10(1):40–6. Article …

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New ways of making babies are on the horizon

The human genome can create cells with a remarkable range of capabilities and shapes. Looked at under the microscope, the enterocytes which line the gut bring to mind the ghosts from a Pac-Man video game. Neurons look like medieval morningstars, but with long hair. But none is more special than…

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Could bovine research unlock women’s fertility secrets?

Lais Barbosa Latorraca’s research into bovine reproduction forms part of a wider study of fertility across species that is funded by the European Commission. For PhD researcher Lais Barbosa Latorraca, basic research enables us “to identify ways to change processes in our favour”. “We need a good foundation to build…

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where is mitochondrial dna found

Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. sFluorescence images of Calcein AM/PI staining in the CsA- or DMSO-treated NPCs. NPC pyroptosis mechanism via mtDNA mediated TLR9-NF-B-NLRP3 axis activation is unclear. In addition, we found that TLR9 recognized and bound mtDNA, which is critical for NF-B and the NLRP3 inflammasome…

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Elevated cell-free mitochondria DNA level of patients with premature ovarian insufficiency | BMC Pregnancy and Childbirth

European Society for Human Reproduction and Embryology Guideline Group on POI, Webber L, Davies M, Anderson R, Bartlett J, Braat D, et al. ESHRE Guideline: management of women with premature ovarian insufficiency. Hum Reprod. 2016; 31(5): 926–37. Welt CK. Primary ovarian insufficiency: a more accurate term for premature ovarian failure….

Continue Reading Elevated cell-free mitochondria DNA level of patients with premature ovarian insufficiency | BMC Pregnancy and Childbirth

Single-cell quantification of ribosome occupancy in early mouse development

PDMS chip fabrication Moulds were 3D-printed by Proto Labs with WaterShed XC 11122 at high resolution (Supplementary Fig. 1). Reusable moulds were assembled by taping 3D-printed molds to glass slides (5″ × 4″; Ted Pella). Sylgard 184 PDMS monomer and curing agent (4019862, Ellsworth Adhesives) were mixed at a 10:1…

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Exploring the Future of Reproductive Medicine: Insights from Renowned Expert Dr. Ian Hardy

Reproductive medicine is a field that constantly evolves, bringing new advancements and possibilities for individuals and couples seeking to start or expand their families. At the forefront of this transformative journey is Dr. Ian Hardy, an esteemed expert in reproductive endocrinology and a trailblazer in the realm of medical research….

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Transcriptomics and the origin of obligate parthenogenesis

Avise J (2008) Clonality: the genetics, ecology, and evolution of sexual abstinence in vertebrate animals. Oxford University Press, USA Book  Google Scholar  Avise JC (2015) Evolutionary perspectives on clonal reproduction in vertebrate animals. Proc Natl Acad Sci USA 112(29):8867–8873 Article  CAS  PubMed  PubMed Central  Google Scholar  Andrews S (2010) FastQC:…

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pMC CMV GFP T2A Puro H1 Scramble shRNA scramble control pare

Catalog number MNSI000A-1 Price Please ask Size 10 ug Shipping Temperature Blue Ice/ Dry Ice Product category Minicircle Technology Description Isotype or positive controls by peptides, antibodies and deactivated samples. Kit Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are…

Continue Reading pMC CMV GFP T2A Puro H1 Scramble shRNA scramble control pare

Indel driven rapid evolution of core nuclear pore protein gene promoters

Lin, D. H. & Hoelz, A. The structure of the nuclear pore complex (an update). Annu. Rev. Biochem. 88, 725–783. doi.org/10.1146/annurev-biochem-062917-011901 (2019). Article  CAS  PubMed  PubMed Central  Google Scholar  Cabal, G. G. et al. SAGA interacting factors confine sub-diffusion of transcribed genes to the nuclear envelope. Nature 441, 770–773 (2006)….

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Restricted Specificity of Xenopus TFIIIA for Transcription of Somatic 5S rRNA Genes: Molecular and Cellular Biology: Vol 24, No 6

Xenopus transcription factor IIIA (TFIIIA) is phosphorylated on serine-16 by CK2. Replacements with alanine or glutamic acid were made at this position in order to address the question of whether phosphorylation possibly influences the function of this factor. Neither substitution has an effect on the DNA or RNA binding activity…

Continue Reading Restricted Specificity of Xenopus TFIIIA for Transcription of Somatic 5S rRNA Genes: Molecular and Cellular Biology: Vol 24, No 6

CHO (Chinese Hamster Ovary) cells were transfected

Transcribed image text: CHO (Chinese Hamster Ovary) cells were transfected with a plasmid containing TRPM8, and the response measured. This was not exactly the same as the TEVC oocyte experiments in the chali paper. but it still was a measure of ion movement the Hor fatio on the y-axis refers…

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A possible role for the 60-kD Ro autoantigen in a discard pathway for defective 5S rRNA precursors.

C A O’Brien and S L Wolin Department of Cell Biology, Yale University School of Medicine, New Haven, Connecticut 06510. Abstract The Ro autoantigen is a 60-kD protein that is usually found in small cytoplasmic RNA-protein complexes known as Ro RNPs. Although the Ro RNPs are abundant and conserved components…

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IJMS | Free Full-Text | Single Copies of the 5S rRNA Inserted into 45S rDNA Intergenic Spacers in the Genomes of Nototheniidae (Perciformes, Actinopterygii)

1. Introduction The ribosomal genes (rDNA) encoding 18S, 5.8S, 28S, and 5S ribosomal RNA (rRNA) are key elements of eukaryotic genomes, since their products are directly involved in the biogenesis and functioning of the ribosomes providing for protein synthesis. Multiple 45S rDNA clusters of the 18S, 5.8S, and 28S rRNA…

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Mitochondrial Inheritance Following Nuclear Transfer: From Cloned Animals to Patients with Mitochondrial Disease

Rojansky R, Cha M-Y, Chan DC (2016) Elimination of paternal mitochondria in mouse embryos occurs through autophagic degradation dependent on PARKIN and MUL1. eLife 5:e17896. doi.org/10.7554/eLife.17896 CrossRef  CAS  PubMed  PubMed Central  Google Scholar  Wei W, Pagnamenta AT, Gleadall N et al (2020) Nuclear-mitochondrial DNA segments resemble paternally inherited mitochondrial DNA…

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Quantitative haplotype-resolved analysis of mitochondrial DNA heteroplasmy in Human single oocytes, blastoids, and pluripotent stem cells

doi: 10.1093/nar/gkad209. Online ahead of print. Chongwei Bi  1 , Lin Wang  1 , Yong Fan  2 , Baolei Yuan  1 , Samhan Alsolami  1 , Yingzi Zhang  1 , Pu-Yao Zhang  3 , Yanyi Huang  4   5 , Yang Yu  3   6 , Juan Carlos Izpisua Belmonte  1   7 , Mo Li  1   8 Affiliations…

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Single-cell individual full-length mtDNA sequencing by iMiGseq uncovers unexpected heteroplasmy shifts in mtDNA editing

doi: 10.1093/nar/gkad208. Online ahead of print. Chongwei Bi  1 , Lin Wang  1 , Yong Fan  2 , Baolei Yuan  1 , Gerardo Ramos-Mandujano  1 , Yingzi Zhang  1 , Samhan Alsolami  1 , Xuan Zhou  1 , Jincheng Wang  3 , Yanjiao Shao  4 , Pradeep Reddy  4 , Pu-Yao Zhang  5 , Yanyi Huang  3   6 , Yang Yu  5…

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Effects of adverse fertility-related factors on mitochondrial DNA in the oocyte: a comprehensive review

The decline of oocyte quality has profound impacts on fertilization, implantation, embryonic development, and the genetic quality of future generations. One factor that is often ignored but is involved in the decline of oocyte quality is mitochondrial DNA (mtDNA) abnormalities. Abnormalities in mtDNA affect the energy production of mitochondria, the…

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Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts

Adelaide Research & Scholarship Please use this identifier to cite or link to this item: hdl.handle.net/2440/137603 Citations Scopus Web of Science® Altmetric Type:  Journal article Title:  Mitochondrial DNA Deficiency and Supplementation in Sus scrofa Oocytes Influence Transcriptome Profiles in Oocytes and Blastocysts Author:  Okada, T.McIlfatrick, S.St. John, J.C. Citation:  International…

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Modeling mitochondrial DNA diseases: from base editing to pluripotent stem-cell-derived organoids

Review doi: 10.15252/embr.202255678. Online ahead of print. Affiliations Expand Affiliations 1 Department of General Pediatrics, Neonatology and Pediatric Cardiology, Medical Faculty, Heinrich Heine University, Düsseldorf, Germany. 2 Unit of Medical Genetics and Neurogenetics, Fondazione IRCCS Istituto Neurologico Carlo Besta, Milan, Italy. Item in Clipboard Review Isabella Tolle et al. EMBO Rep….

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On Safety in Engineering Embryos: Engage the Brakes

“Golden mitochondria” by National Institutes of Health(NIH) is licensed under CC BY-NC 2.0. A controversial embryo engineering technique turns out not to work as planned. It is touted as a way to prevent the births of children with mitochondrial disease, and a few fertility clinics have been using it – without much evidence…

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KEGG T01002: 16333

Entry 16333             CDS       T01002                                  Symbol Ins1, Ins-1, Ins2-rs1 Name (RefSeq) insulin I   KO K04526   insulin Organism mmu  Mus musculus (house mouse) Pathway mmu04010   MAPK signaling pathway mmu04014   Ras signaling pathway mmu04015   Rap1 signaling pathway mmu04022   cGMP-PKG signaling pathway mmu04066   HIF-1 signaling pathway mmu04068   FoxO signaling pathway mmu04072   Phospholipase D signaling pathway mmu04114   Oocyte…

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BOMIPROT

1 Bomi8228 Probetacellulin Q9TTC5 2 Bomi5121 Dimethylaniline monooxygenase [N-oxide-forming] 3/Dimethylaniline oxidase 3/Hepatic flavin-containing monooxygenase 3/Trimethylamine monooxygenase Q8HYJ9 3 Bomi10437 Uterine milk protein P46201 4 Bomi9179 Signal transducer and activator of transcription 5A/Mammary gland factor Q95115 5 Bomi9003 Selenoprotein P P49907 6 Bomi3613 Acetyl-CoA carboxylase 1 Q9TTS3 7 Bomi5003 Cytoskeleton-associated protein…

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Paternal aging impacts mitochondrial DNA content and telomere length in mouse embryos

Advanced paternal age results in low fertility, regardless of natural or assisted reproductive technology (Brandt et al., 2019, Kovac et al., 2013). Furthermore, the offspring of aged male subjects reportedly exhibit an increased risk of childhood cancer, psychological disease, and obstetrical complications (Brandt et al., 2019, Kovac et al., 2013)….

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Assessing spermatozoal small ribonucleic acids and their relationship to blastocyst development in idiopathic infertile males

Vander, B. M. & Wyns, C. Fertility and infertility: Definition and epidemiology. Clin. Biochem. 62, (2018). Sun, H. et al. Global, regional, and national prevalence and disability-adjusted life-years for infertility in 195 countries and territories, 1990–2017: Results from a global burden of disease study, 2017. Aging 11, 10952 (2019). Article …

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The comprehensive detection of miRNA and circRNA in the regulation of intramuscular and subcutaneous adipose tissue of Laiwu pig

Zhou, G. et al. Global comparison of gene expression profiles between intramuscular and subcutaneous adipocytes of neonatal landrace pig using microarray. Meat. Sci. 86(2), 440–450 (2010). CAS  PubMed  Article  Google Scholar  Alfaia, C. M. et al. Current feeding strategies to improve pork intramuscular fat content and its nutritional quality. Adv….

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Using RFLP-PCR, Mini Sequencing and STR Techniques in Preimplantation Diagnosis of Spinal Muscular Dystrophy in Vietnam

Background: Spinal Muscular Atrophy (SMA) is one of the most common autosomal recessive disorders in children after Duchenne muscular dystrophy affecting approximately 1 in 11,000 live births. There is no effective therapy for SMA; however, broadening our knowledge about the molecular genetics of the disease is resultant to develop potential…

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BMP-6 and SMAD4 gene expression is altered in cumulus cells from women with endometriosis-associated infertility

Introduction Oocyte competence and quality depend on communication between the oocyte and the cumulus and theca cells. In the preantral phase, the members of the transforming growth factor β (TGF-β) superfamily are responsible for this communication and play an important role in folliculogenesis. Members of the TGF-β superfamily are related…

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Mechanisms involved in the transmission of genetic diseases from mother to child

Mitochondria are the organelles that produce most of the chemical energy needed by the cell. Mitochondrial DNA (mtDNA) contains 16,569 nucleotides that can be mutated. Some of these mutations can lead to the development of mitochondrial diseases. While nuclear DNA (the well-known double helix, which encodes most of the genome)…

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Research reveals mechanism involved in transm

image: Immature mouse egg at a stage prior to ovulation, with mitochondria stained red view more  Credit: Marcos Roberto Chiaratti A research article published in the journal Science Advances describes a mechanism that helps explain how certain kinds of genetic disorders known as mitochondrial diseases are transmitted from mother to child. The study it reports…

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Role of GPs in changing landscape of fertility treatment

PRIMARY care has for years been the cornerstone of the Australian health care system and GPs represent an integral part of it. As they are frequently responsible for the first, and sometimes only, contact of individuals with the health care system, they can profoundly affect a patient’s experience, expectations and…

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Two PhD scholarships in Transporter Protein Engineering at DTU Biosustain

The Yeast Metabolic Engineering group develops novel yeast cell factories to produce sustainable chemicals and natural products. We apply advanced genome editing, metabolic modeling, transporter engineering, and ‘omic data analyses for data-guided strain design. We are looking for two motivated, talented PhD students to join our team to study small…

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Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture

doi: 10.1016/j.mex.2021.101419. eCollection 2021. Affiliations Expand Affiliations 1 Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA. 2 Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA. Free PMC article Item in Clipboard Anil K Challa et al….

Continue Reading Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture