Categories
Tag: p53
eS6 – Ribosomal Proteins
A, 3-D models of human ribosomal subunits based on PDB entries 3J3D and 3J3A (for SSU r-proteins), and on 3J3F and 3J3B (for LSU r-proteins) highlighting the position of individual r-proteins on mature subunits. On small subunits, the 18S rRNA is shown in grey; on large subunits, the 5S, 5.8S…
NM_000546.6(TP53):c.743G>A (p.Arg248Gln) AND Lymphoma – ClinVar
NM_000546.6(TP53):c.743G>A (p.Arg248Gln) AND Lymphoma Based on: 1 submission [Details] Record status: current Accession: RCV000790860.7 Allele description NM_000546.6(TP53):c.743G>A (p.Arg248Gln) Gene: TP53:tumor protein p53 [Gene – OMIM – HGNC] Variant type: single nucleotide variant Cytogenetic location: 17p13.1 Genomic location: Preferred name: NM_000546.6(TP53):c.743G>A (p.Arg248Gln) Other names: p.R248Q:CGG>CAG HGVS: NC_000017.11:g.7674220C>T NG_017013.2:g.18331G>A NM_000546.6:c.743G>AMANE SELECT NM_001126112.3:c.743G>A…
Sequential deregulation of histone marks, chromatin accessibility and gene expression in response to PROTAC-induced degradation of ASH2L
Loss of ASH2L prevents cell proliferation We have studied the molecular and cellular consequences of Ash2l loss in mouse embryo fibroblasts (MEFs) with floxed Ash2l alleles and an inducible Cre-ER recombinase (iMEF-Ash2lfl/fl-Cre-ER). While the knockout of Ash2l was rapid, the downstream effects, including the decrease in promoter-associated H3K4me3, altered gene…
PRRG4 regulates mitochondrial function and promotes migratory behaviors of breast cancer cells through the Src-STAT3-POLG axis | Cancer Cell International
Sung H, Ferlay J, Siegel RL, Laversanne M, Soerjomataram I, Jemal A, Bray F. Global cancer statistics 2020: GLOBOCAN estimates of incidence and mortality Worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2021;71(3):209–49. doi.org/10.3322/caac.21660. Article PubMed Google Scholar Hu G, Chong RA, Yang Q, Wei Y, Blanco…
Identification of Differentially Expressed Genes in Human Colorectal Cancer Using RNASeq Data Validated on the Molecular Level with Real-Time PCR
Allam RM, Al-Abd AM, Khedr A, Sharaf OA, Nofal SM, Khalifa AE, Mosli HA, Abdel-Naim AB (2018) Fingolimod interrupts the cross talk between estrogen metabolism and sphingolipid metabolism within prostate cancer cells. Toxicol Lett 291:77–85 Article CAS PubMed Google Scholar Andrews S et al (2010) FastQC: a quality control tool…
Formononetin | FGFR2 inhibitor | Axon 4067
All All Products Enzymes Receptors Ion Channels Research Areas Oxidoreductases Transferases Lyases Isomerases Ligases Nuclear Receptors Ligand-gated ATP-Sensitive Angiogenesis Apoptosis Cardiovascular Cell Cycle Regulation Cell Signaling & Oncology CNS Diabetes & Metabolism Epigenetics Immunology Miscellaneous Pain & Inflammation Stem Cell Oxidases MAO Cys-loop, cationic Cis-Trans Isomerase CypA DNA Topoisomerase Synthetases…
CHEK2 is a potential prognostic biomarker associated with immune infiltration in clear cell renal cell carcinoma
CHEK2 expression increased in cancer tissues First, the expression levels of CHEK2 in different tumor types in the TIMER database were evaluated. All tumors except for chromophobe renal cell carcinoma (KICH) showed elevated levels of CHEK2 expression compared to normal cells (Fig. 1A). Similarly, CHEK2 protein expression was upregulated in all…
Uncovering myocardial infarction genetic signatures using GWAS exploration in Saudi and European cohorts
Benjamin, E. J. et al. Heart disease and stroke statistics-2019 update: A report from the American Heart Association. Circulation 139, e56–e528 (2019). Article PubMed Google Scholar Yusuf, S. et al. Effect of potentially modifiable risk factors associated with myocardial infarction in 52 countries (the INTERHEART study): Case-control study. Lancet 364,…
Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition
Patients, tumour Implantation, PDX expansion and characterization The study was conducted according to the Declaration of Helsinki. Tumours were collected from EC patients following Human Research Ethics Committee (HREC) approval (HREC/15/MHS/127) and QUT HREC (#1500000169, 1500000323). All participants provided written informed consent that their excess tissue could be used for…
Gene Therapy: Unlocking Innovations in Cancer Treatment, Detection, and Drug Development
The history of using genes as a tool to treat humans goes back nearly five decades, marking a pivotal moment in medical science. In 1972, the concept of gene therapy was first considered as a form of treatment. This groundbreaking idea was introduced in a paper titled “Gene therapy for…
HER2 low expression breast cancer subtyping and their correlation with prognosis and immune landscape based on the histone modification related genes
Barzaman, K. et al. Breast cancer: Biology, biomarkers, and treatments. Int. Immunopharmacol. 84, 106535 (2020). Article CAS PubMed Google Scholar Siegel, R. L., Miller, K. D., Wagle, N. S. & Jemal, A. Cancer statistics, 2023. CA Cancer J. Clin. 73(1), 17–48 (2023). Article PubMed Google Scholar Mueller, C., Haymond, A.,…
Future-focused therapeutics | Scientist Live
Base editing is bringing the next breakthrough in cell and gene therapy, explains Michelle Fraser. Cell and gene therapies (CGTs) hold the promise to transform medicine for patients globally. These therapies work by correcting DNA to treat a genetic disease, or by modifying cells from the immune system to specifically…
Bioinformatics analysis of immune characteristics in tumors with alternative carcinogenesis pathways induced by human papillomaviruses | Virology Journal
de Martel C, Georges D, Bray F, Ferlay J, Clifford GM. Global burden of cancer attributable to infections in 2018: a worldwide incidence analysis. Lancet Glob Health. 2020;8:e180–90. Article PubMed Google Scholar McKaig RG, Baric RS, Olshan AF. Human papillomavirus and head and neck cancer: epidemiology and molecular biology. Head…
Microbial gene expression analysis of healthy and cancerous esophagus uncovers bacterial biomarkers of clinical outcomes
Yang J, Liu X, Cao S, Dong X, Rao S, Cai K. Understanding esophageal cancer: the challenges and opportunities for the next decade. Front Oncol. 2020;10:1727. Article PubMed PubMed Central Google Scholar Li J, Xu J, Zheng Y, Gao Y, He S, Li H, et al. Esophageal cancer: epidemiology, risk…
NOTCH1 mutations predict superior outcomes, NSCLC
Introduction Lung cancer remains the leading cause of cancer-related mortality worldwide, and the majority are non-small-cell lung cancer (NSCLC).1,2 Genetic variation is a typical feature of NSCLC that drives cancer initiation and progression.3 Understanding the role of mutated genes in NSCLC is the basis of the development of novel treatment…
Altered Glia-Neuron Communication in Alzheimer’s Disease Affects WNT, p53, and NFkB Signaling Determined by snRNA-seq
Abstract Background: Alzheimer’s disease is the most common cause of dementia and is characterized by amyloid-β plaques, tau neurofibrillary tangles, and neuronal loss. Although neuronal loss is a primary hallmark of Alzheimer’s disease, it is known that non-neuronal cell populations are ultimately responsible for maintaining brain homeostasis and neuronal health…
Mitophagy in human health, ageing and disease
Palikaras, K., Lionaki, E. & Tavernarakis, N. Mechanisms of mitophagy in cellular homeostasis, physiology and pathology. Nat. Cell Biol. 20, 1013–1022 (2018). Article CAS PubMed Google Scholar Palikaras, K., Lionaki, E. & Tavernarakis, N. Coordination of mitophagy and mitochondrial biogenesis during ageing in C. elegans. Nature 521, 525–528 (2015). Article …
Potential use of iPSCs for disease modeling, drug screening, and cell-based therapy for Alzheimer’s disease | Cellular & Molecular Biology Letters
Tarawneh R, Holtzman DM. The clinical problem of symptomatic Alzheimer disease and mild cognitive impairment. Cold Spring Harb Perspect Med. 2012;2(5): a006148. Article PubMed PubMed Central Google Scholar van der Flier WM, Scheltens P. Epidemiology and risk factors of dementia. J Neurol Neurosurg Psychiatry. 2005;76(suppl 5):v2-7. Article PubMed PubMed Central …
Functions Support the New RNA Gene Paradigm
Photo credit: THAVIS 3D via Unsplash. In recent days I have covered a new paper published in BioEssays by Australian molecular biologist John Mattick who cites the need for a “paradigm shift” in biology away from the concept of junk DNA (see here, here, and here). I recounted how this shift is being driven by…
Global genetic diversity, introgression, and evolutionary adaptation of indicine cattle revealed by whole genome sequencing
Loftus, R. T., MacHugh, D. E., Bradley, D. G., Sharp, P. M. & Cunningham, P. Evidence for two independent domestications of cattle. Proc. Natl Acad. Sci. USA 91, 2757–2761 (1994). Article ADS CAS PubMed PubMed Central Google Scholar Verdugo Marta, P. et al. Ancient cattle genomics, origins, and rapid turnover…
KEGG T01001: 637
Entry 637 CDS T01001 Symbol BID, FP497 Name (RefSeq) BH3 interacting domain death agonist KO K04726 BH3 interacting domain death agonist Organism hsa Homo sapiens (human) Pathway hsa01524 Platinum drug resistance hsa04071 Sphingolipid signaling pathway hsa04115 p53 signaling pathway hsa04210 Apoptosis hsa04215 Apoptosis – multiple species hsa04217 Necroptosis hsa04650 Natural killer cell mediated…
Maintenance Selinexor Shows PFS in TP53 Wild-type Endometrial Cancer
Selinexor did not show a clinically meaningful improvement in the general study population. Maintenance selinexor (Xpovio) showed a significant progression-free survival (PFS) benefit compared with placebo in a subgroup of patients with TP53 wild-type advanced/recurrent endometrial cancer, according to findings from the phase 3 ENGOT-EN5/GOG-3055/SIENDO trial (NCT03555422).1,2 Data from the…
Decline of DNA damage response along with myogenic differentiation
Introduction Proper functioning of all living organisms depends on the faithful maintenance and transmission of genomic information stored in the molecule of DNA. However, DNA integrity is continuously challenged by a variety of endogenous and exogenous agents causing DNA lesions which have a critical impact on cellular activities and homeostasis….
Selinexor Maintenance Leads to PFS Benefit in TP53 Wild-Type Advanced/Recurrent Endometrial Cancer
Maintenance therapy with selinexor (Xpovio) improved progression-free survival (PFS) compared with placebo in patients with TP53 wild-type advanced or recurrent endometrial cancer, regardless of microsatellite instability (MSI) status, according to long-term follow-up data from the phase 3 ENGOT-EN5/GOG-3055/SIENDO trial (NCT03555422)presented at the 2023 IGCS Annual Global Meeting.1 At a median…
Molecular-based classification of endometrial carcinoma in Northern Thailand: impact on prognosis and potential for implementation in resource-limited settings | BMC Women’s Health
Study population The study cohort included women diagnosed with endometrial carcinoma at Chiang Mai University Hospital during January 2015 to December 2017. Inclusion criteria were patients with informed consent, available hysterectomy specimens for evaluation, and available clinical information and pathological results. We excluded individual without histologic materials and formalin-fixed paraffin-embedded…
IJMS | Free Full-Text | CRISPR/Cas9-Mediated Genome Editing in Cancer Therapy
1. Introduction Recently, the morbidity and mortality rates of cancer have been increasing rapidly, posing a significant threat to human health. Cancer, a refractory and multifaceted disease, fundamentally originates from a cumulative series of mutations in the cellular genome and epigenome. These mutations activate oncogenes and deactivate tumor suppressors, leading…
Bioinformatics analysis of immune cell infiltration patterns and potential diagnostic markers in atherosclerosis
A database in the Gene Set Enrichment Analysis (GSEA; www.gsea-msigdb.org/gsea/msigdb/index.jsp) platform10,11 was used to identify 134 GLN metabolism-associated genes. Weighted gene co-expression network analysis (WGCNA) and module screening GLN-associated gene sets were investigated using WGCNA. The results demonstrated that when the weighted value was 24 (Fig. 1A), scale independence was greater…
Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2
Ethic statement The Clinical Research Ethics Review Committee of Kyushu University Hospital approved the study (permit no. 29-43, 30-268, and 700-00). Written informed consent for the current research project was obtained before the tumor tissue, and a blood sample were harvested. This study was also conducted according to the principles…
The power and potential of mitochondria transfer
Roger, A. J., Muñoz-Gómez, S. A. & Kamikawa, R. The origin and diversification of mitochondria. Curr. Biol. 27, R1177–R1192 (2017). Article CAS PubMed Google Scholar Monzel, A. S., Enríquez, J. A. & Picard, M. Multifaceted mitochondria: moving mitochondrial science beyond function and dysfunction. Nat. Metab. 5, 546–562 (2023). Article PubMed …
Next generation sequencing uncovers multiple miRNAs associated molecular targets in gallbladder cancer patients
Hundal, R. & Shaffer, E. A. Gallbladder cancer: Epidemiology and outcome. Clin. Epidemiol. 6, 99 (2014). PubMed PubMed Central Google Scholar Lazcano-Ponce, E. C. et al. Epidemiology and molecular pathology of gallbladder cancer. CA Cancer J. Clin. 51(6), 349–364 (2001). Article CAS PubMed Google Scholar World Health Organization International Agency…
Karyopharm Therapeutics Reports Q3 2023 Financial Results, Highlights Strong Position for Growth By Investing.com
© Reuters. Karyopharm Therapeutics (NASDAQ: NASDAQ:) has reported its financial results for the third quarter of 2023, noting a strong position for growth backed by its pipeline of innovative oral selective inhibitors and nuclear export. The company expects total revenues of $145 million to $160 million in 2023, and anticipates…
ncRNA | Free Full-Text | Dysregulation of Non-Coding RNAs: Roles of miRNAs and lncRNAs in the Pathogenesis of Multiple Myeloma
4. Dysregulation of lncRNAs in MM In recent years, numerous studies have focused on the role of lncRNAs in MM pathogenesis. Since then, the role of aberrantly expressed lncRNAs in MM has been reported, highlighting the potential in clinical applications. For instance, lncRNAs participate in the differentiation of erythroid, myeloid,…
VIDEO: Advancements in DLBCL subtyping
Editor’s note: This is an automatically generated transcript. Please notify editor@healio.com if there are concerns regarding accuracy of the transcription. The truth is, not all of these things are absolutely clear for prime time, meaning that most of these things don’t end up changing how we consider first-line therapy. Some…
DNA damage response(DDR): a link between cellular senescence and human cytomegalovirus | Virology Journal
Hayflick L, Moorhead PS. The serial cultivation of human diploid cell strains. Exp Cell Res. 1961;25:585–621. Article CAS PubMed Google Scholar Hayflick L. THE LIMITED IN VITRO LIFETIME OF HUMAN DIPLOID CELL STRAINS. Exp Cell Res. 1965;37:614–36. Article CAS PubMed Google Scholar Schmitt CA, Tchkonia T, Niedernhofer LJ, Robbins PD,…
CRISPR/Cas9-mediated knock-in of BRCA1/2 mutations restores response to olaparib in pancreatic cancer cell lines
Rawla, P., Sunkara, T. & Gaduputi, V. Epidemiology of pancreatic cancer: Global trends, etiology and risk factors. World J. Oncol. 10, 10–27 (2019). Article PubMed PubMed Central Google Scholar Lambert, A. et al. An update on treatment options for pancreatic adenocarcinoma. Ther. Adv. Med. Oncol. 11, 1758835919875568 (2019). Article PubMed …
CRISPR Reveals PTEN and P53 as Key Genes in Endometrial Cancer Development
– Advertisement – CRISPR helps uncover endometrial cancer genes Endometrial cancer is the sixth most common cancer in women and the most frequent type of cancer in the female reproductive tract. Mutations in the PTEN and P53 genes have been found to be responsible for the development of endometrial cancer….
CRISPR helps uncover endometrial cancer genes
With more than 400,000 new cases diagnosed worldwide each year, endometrial cancer is the sixth most common cancer in women and the most frequent type of cancer in the female reproductive tract. It is thought to begin in a layer of cells that form the lining of the uterus, called…
Bi-allelic truncating variants in CASP2 underlie a neurodevelopmental disorder with lissencephaly
Di Donato N, Chiari S, Mirzaa GM, Aldinger K, Parrini E, Olds C, et al. Lissencephaly: expanded imaging and clinical classification. Am J Med Genet A. 2017;173:1473–88. Article PubMed PubMed Central Google Scholar Guerrini R, Dobyns WB. Malformations of cortical development: clinical features and genetic causes. Lancet Neurol. 2014;13:710–26. Article …
What is Simian Virus 40 DNA?
What is Simian Virus 40 DNA Simian Virus 40 (SV40) DNA is a small, circular, double-stranded DNA virus that belongs to the polyomavirus family. It was first discovered in 1960 as a contaminant in polio vaccines derived from monkey kidney cells. SV40 gained significant attention due to its potential association…
Genome-wide epigenetic dynamics during postnatal skeletal muscle growth in Hu sheep
Global changes of the transcriptome during postnatal muscle growth The number of myofibers is thought to remain fixed following birth, muscle fiber hypertrophy growth is the primaryway of muscle growth. The average CSA is generally used to measure the hypertrophy of skeletal muscle fibers. To systematically identify the growth of postnatal…
Intrinsic targeting of host RNA by Cas13 constrains its utility
Abudayyeh, O. O. et al. C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector. Science 353, aaf5573 (2016). Article PubMed PubMed Central Google Scholar Terns, M. P. CRISPR-based technologies: impact of RNA-targeting systems. Mol. Cell 72, 404–412 (2018). Article CAS PubMed PubMed Central Google Scholar Abudayyeh, O. O. et al….
Retraction Note to: Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice
Title Retraction Note to: Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice Creator Safdar, Adeel; Khrapko, Konstantin; Flynn, James M; Saleem, Ayesha; De Lisio, Michael; Johnston, Adam P W; Kratysberg, Yevgenya; Samjoo, Imtiaz A; Kitaoka, Yu; Ogborn, Daniel I; Little, Jonathan P.; Raha, Sandeep; Parise, Gianni; Akhtar, Mahmood; Hettinga,…
Macrophage polarization and metabolism in atherosclerosis
Organization WH the top 10 causes of death. www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death, 2019. Rana JS, Khan SS, Lloyd-Jones DM, Sidney S. Changes in mortality in Top 10 causes of death from 2011 to 2018. J Gen Intern Med. 2021;36:2517–8. Article PubMed Google Scholar Libby P. The changing landscape of atherosclerosis. Nature. 2021;592:524–33. Article …
PLA2G7 gene polymorphism in gestational diabetes mellitus
Introduction Gestational diabetes mellitus (GDM) is a general term for abnormal glucose tolerance, abnormal fasting blood glucose, and diabetes detected or developed during pregnancy, excluding diabetes that already existed before pregnancy. According to the International Diabetes Federation, the global prevalence of GDM in 2021 was approximately 16.7% and has become…
BMAL1 modulates senescence programming via AP
image: Figure 4. BMAL1 contributes to AP-1 mediated transcriptional control of the senescence program and confers resistance to apoptosis. view more Credit: 2023 Jachim et al. “In this study, we discovered that senescence alters the amplitude and period of core circadian clock components, most notably BMAL1 […]” BUFFALO, NY- October 16,…
Moving beyond protein structure | Opinion
It’s a bit ironic that, just as methods for deducing the crystal structures of protein molecules have become supercharged, we’ve started to discover they might not always help very much for developing drugs that target proteins. First there was synchrotron radiation, and now free-electron lasers, as sources of ultrabright x-ray…
G. lucidum triterpenes restores intestinal flora balance in non-hepatitis B virus-related hepatocellular carcinoma: evidence of 16S rRNA sequencing and network pharmacology analysis
. 2023 Sep 18:14:1197418. doi: 10.3389/fphar.2023.1197418. eCollection 2023. Affiliations Expand Affiliation 1 Chongqing Three Gorges Medical College, Chongqing Key Laboratory of Development and Utilization of Genuine Medicinal Materials in Three Gorges Reservoir Area, Chongqing, China. Free PMC article Item in Clipboard Wei Xiong et al. Front Pharmacol. 2023. Free PMC article…
Histone regulator KAT2A acts as a potential biomarker related to tumor microenvironment and prognosis of diffuse large B cell lymphoma | BMC Cancer
Zhang Y, Tan H, Daniels JD, Zandkarimi F, Liu H, Brown LM, et al. Imidazole Ketone Erastin induces ferroptosis and slows Tumor Growth in a mouse lymphoma model. Cell Chem Biol. 2019;26(5):623–33e9. Article CAS PubMed PubMed Central Google Scholar Hartert KT, Wenzl K, Krull JE, Manske M, Sarangi V, Asmann…
Somatic evolution of marine transmissible leukemias in the common cockle, Cerastoderma edule
Murchison, E. P. Clonally transmissible cancers in dogs and Tasmanian devils. Oncogene 27, S19–S30 (2009). Article Google Scholar Metzger, M. J. & Goff, S. P. A sixth modality of infectious disease: contagious cancer from devils to clams and beyond. PLoS Pathog. 12, e1005904 (2016). Article PubMed PubMed Central Google Scholar …
Novel Drugs May Introduce ‘New Era’ of Metastatic Prostate Cancer Treatment
The “exciting” development of novel agents, ranging from androgen receptor (AR) inhibitors to bispecific T cells may lead to a new “era of treatment” for patients with metastatic prostate cancer, according to Neeraj Agarwal, MD. Neeraj Agarwal, MD, a professor of medicine, presidential endowed chair of cancer research, and director…
Clinical Behavior and Molecular Landscape of Stage I p53-abnormal Low-Grade Endometrioid Endometrial Carcinomas | Clinical Cancer Research
Citation Amy Jamieson, Lisa Vermij, Claire J.H. Kramer, Jan J. Jobsen, Ina Jürgenliemk-Schulz, Ludy Lutgens, Jan Willem Mens, Marie A.D. Haverkort, Annerie Slot, Remi A. Nout, Jan Oosting, Joseph Carlson, Brooke E. Howitt, Philip P.C. Ip, Sigurd F. Lax, W. Glenn McCluggage, Naveena Singh, Jessica N. McAlpine, Carien L. Creutzberg,…
IJMS | Free Full-Text | CRISPR-Cas9 Direct Fusions for Improved Genome Editing via Enhanced Homologous Recombination
Over the past decade, CRISPR-Cas9 has found widespread application in loss-of-function mutations, but precise genetic engineering for gene correction or gene replacement therapies has lagged behind. In vivo correction using CRISPR-Cas9 to replace genetic mutations by HR is highly challenging, and very few studies have managed to achieve this [31,32]….
Induced Pluripotent Stem Cells (iPSCs) and Genetic Engineering
Benabdellah, K., Sánchez-Hernández, S., Aguilar-González, A., Maldonado-Pérez, N., Gutierrez-Guerrero, A., Cortijo-Gutierrez, M., Ramos-Hernández, I., Tristán-Manzano, M., Galindo-Moreno, P., Herrera, C., & Martin, F. (2020). Genome-edited adult stem cells: Next-generation advanced therapy medicinal products. Stem Cells Translational Medicine, 9(6), 674–685. doi.org/10.1002/sctm.19-0338 Chang, C. W., Lai, Y. S., Pawlik, K. M., Liu,…
Precision RNA base editing with engineered and endogenous effectors
Gray, M. W. Evolutionary origin of RNA editing. Biochemistry 51, 5235–5242 (2012). Article CAS PubMed Google Scholar Covello, P. & Gray, M. On the evolution of RNA editing. Trends Genet. 9, 265–268 (1993). Article CAS PubMed Google Scholar Yablonovitch, A. L., Deng, P., Jacobson, D. & Li, J. B. The…
Strong associations of telomere length and mitochondrial copy number with suicidality and abuse history in adolescent depressed individuals
Emslie GJ, Ventura D, Korotzer A, Tourkodimitris S. Escitalopram in the treatment of adolescent depression: a randomized placebo-controlled multisite trial. J Am Acad Child Adolesc Psychiatry. 2009;48:721–9. Article PubMed Google Scholar Daly M. Prevalence of depression among adolescents in the U.S. from 2009 to 2019: analysis of trends by sex,…
Immune and cytokine alterations and RNA-sequencing analysis in gestational tissues from pregnant women after recovery from COVID-19 | BMC Infectious Diseases
Baseline characteristics of study participants A total of 40 pregnant women were enrolled into the study, with 13 in the convalescent group and 27 in the control group. During COVID-19 infection in the 13 convalescent women, one had a temporary loss of taste, one had occasional palpitations, and three had…
Recent research in prostate cancer: Breakthroughs and innovations
Prostate cancer is the second most commonly occurring cancer in men. More than 1.4 million people were diagnosed with prostate cancer in 2020, according to the World Cancer Research Fund International. While some prostate cancers develop too slowly, to the extent that some people will not need any treatment during…
I need to retrieve a set of protein and mRNA sequences
Using EntrezDirect: $ esearch -db gene -query ‘7157’ | elink -db gene -target nuccore -name gene_nuccore_refseqrna | efetch -format fasta | grep “>” >NR_176326.1 Homo sapiens tumor protein p53 (TP53), transcript variant 14, non-coding RNA >NM_001407264.1 Homo sapiens tumor protein p53 (TP53), transcript variant 10, mRNA >NM_001407263.1 Homo sapiens tumor…
Aedes aegypti Argonaute 2 controls arbovirus infection and host mortality
Generation and characterization of Ago2 knockout mutants To demonstrate the essential function of Ago2 in the Ae. aegypti siRNA pathway, we used CRISPR/Cas9 to knock out Ago2 and then investigated its role in defending against arbovirus infection (Fig. 1a). Ae. aegypti Ago2 (AeAgo2) has four exons and four predicated functional domains,…
Precision treatment of cholangiocarcinoma: current strategies and future directions
Cholangiocarcinoma is an aggressive malignant tumor originating from the biliary tract and can be divided into three subtypes based on anatomical location: intrahepatic cholangiocarcinoma, perihepatic cholangiocarcinoma, and distal cholangiocarcinoma [1]. Although progress has been made in the field of cholangiocarcinoma treatment in recent years, cholangiocarcinoma mortality remains high [2]. Although…
Novel Therapies Are Paving the Way to Better Patient Outcomes
This World Lymphoma Awareness Day, it’s important to remember that while the disease is increasingly more common, multiple novel therapies are available to better combat this disease. According to the National Institute of Health Surveillance, Epidemiology, and End Results (SEER) program, the estimated number of new cases of non-Hodgkin lymphoma…
Growth phase estimation for abundant bacterial populations sampled longitudinally from human stool metagenomes
El Aidy, S., Hooiveld, G., Tremaroli, V., Bäckhed, F. & Kleerebezem, M. The gut microbiota and mucosal homeostasis: colonized at birth or at adulthood, does it matter? Gut Microbes 4, 118–124 (2013). Article PubMed PubMed Central Google Scholar Martin, A. M., Sun, E. W., Rogers, G. B. & Keating, D….
Combination of PARP inhibitor and CDK4/6 inhibitor modulates cGAS/STING-dependent therapy-induced senescence and provides “one-two punch” opportunity with anti-PD-L1 therapy in colorectal cancer
doi: 10.1111/cas.15961. Online ahead of print. Tao Wang 1 2 , Weizhen Liu 1 , Qian Shen 3 , Ruikang Tao 4 , Chengguo Li 1 , Qian Shen 1 , Yao Lin 1 , Yongzhou Huang 1 , Lei Yang 1 , Gengchen Xie 1 , Jie Bai 1 , Ruidong Li 1 , Lulu Wang 5 , Kaixiong Tao 1…
Integrated bioinformatics analysis of noncoding RNAs with tumor immune microenvironment in gastric cancer
Sung, H. et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 71, 209–249. doi.org/10.3322/caac.21660 (2021). Article PubMed Google Scholar Siegel, R. L., Miller, K. D., Fuchs, H. E. & Jemal, A. Cancer statistics, 2022. CA A…
First-in-Class Targeted MicroRNA Therapy Blocks Tumor Growth in Mice
Researchers at Purdue University have developed a new type of cancer therapy that uses a modified microRNA, miR-34a, to block cancer cell division. In preclinical tests in mice, treatment using the fully modified, tumor-targeting miR-34a halted tumor growth for the 21-day evaluation period, and resulted in complete cures in some…
Whale Mutation Rates Surpass Previous Estimates
An international team of marine scientists, led by the University of Groningen in the Netherlands and the Center for Coastal Studies in the USA, has studied the DNA of family groups from four different whale species to estimate their mutation rates. The results revealed much higher mutation rates than previously…
RefSeq: NP_001154929
LOCUS NP_001154929 155 aa linear ROD 03-MAY-2023 DEFINITION core-binding factor subunit beta isoform 3 [Mus musculus]. ACCESSION NP_001154929 VERSION NP_001154929.1 DBSOURCE REFSEQ: accession NM_001161457.1 KEYWORDS RefSeq. SOURCE Mus musculus (house mouse) ORGANISM Mus musculus Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus;…
“Mouse Modeling of Pancreatic Ductal Adenocarcinoma (PDAC); Search for ” by Kyoko Kojima
Degree Name by School School of Medicine [MED]; Doctor of Philosophy (PhD) Abstract PDAC is a highly malignant neoplasm that carries a very poor prognosis. PDAC development is a multistage transformation process that involves multiple genetic alterations that include activation of EGFR/HER2 and KRAS, and loss-of-function mutations in INK4A/ARF, p53…
Repression of rRNA gene transcription by endothelial SPEN deficiency normalizes tumor vasculature via nucleolar stress
Human cancers induce a chaotic, dysfunctional vasculature that promotes tumor growth and dampens most current therapies, but the underlying mechanism has been unclear. Here we show that SPEN (split end), a transcription repressor, coordinates ribosome RNA (rRNA) synthesis in endothelial cells (ECs) and is required for physiological and tumor angiogenesis….
Celltrion gets OK partial approval for phase 3 of biosimilar in Europe
South Korean biopharmaceutical company Celltrion Inc. announced on Tuesday that it has received approval from the European Medicines Agency (EMA) for Part 1 of the phase 3 clinical trial plan of the Ocrevus biosimilar CT-P53. In this phase 3 trial, Celltrion will conduct comparative studies of efficacy, pharmacokinetics, and safety…
Design of Cyclic Peptides Targeting Protein-Protein Interactions using AlphaFold
Abstract More than 930,000 protein-protein interactions (PPIs) have been identified in recent years, but their physicochemical properties differ from conventional drug targets, complicating the use of conventional small molecules as modalities. Cyclic peptides are a promising modality for targeting protein-protein interactions (PPIs), but it is difficult to predict the structure…
Impact of Smad4 and p53 mutations on the prognosis of patients with pancreatic ductal adenocarcinoma undergoing chemotherapy
Background: This prospective cohort study evaluated the feasibility of using endoscopic ultrasound-guided fine-needle biopsy (EUS-FNB) samples for comprehensive mutational analysis of cancer-related genes using microtissues. Methods: Fifty patients with suspected pancreatic cancer presenting consecutively at the Kindai University Hospital between January 2018 and January 2019 were enrolled. Cancerous tissues from…
A Novel Method Transforms Human Skin Cells Into Pluripotent Stem Cells
Description Researchers from the Indian Institute of Technology (IIT) Guwahati and the Christian Medical College in Vellore have successfully devised a technology for transforming ordinary human skin cells into pluripotent stem cells known as Induced Pluripotent Stem Cells (iPSCs). Unlike traditional procedures that involve collecting stem cells from embryos or…
Breakthrough Method Converts Human Skin Cells into Pluripotent Stem Cells
August 18, 2023 Researchers from the Indian Institute of Technology (IIT) Guwahati and Christian Medical College, Vellore, have successfully developed a technique to transform regular human skin cells into pluripotent stem cells, known as Induced Pluripotent Stem Cells (iPSCs). Unlike conventional methods that involve extracting stem cells from embryos or…
Dynamic 3D genome reorganization during senescence: defining cell states through chromatin
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Researchers At IIT Guwahati Produces Pluripotent Stem Cells From Skin Cells
Researchers At IIT Guwahati Produces Pluripotent Stem Cells From Skin Cells | IANS Guwahati: Researchers from the Indian Institute of Technology (IIT) Guwahati in collaboration with scientists from Christian Medical College, Vellore, have reported a method to convert regular human skin cells into pluripotent stem cells. Explaining the basics of…
Skin Cells Transformed Into Pluripotent Stem Cells For Regenerative Therapy
IIT Guwahati Leads in Producing Pluripotent Stem Cells from Skin Cells GUWAHATI, August 16, 2023: In a monumental stride for regenerative medicine, a collaborative research effort led by the Indian Institute of Technology Guwahati (IITG) has successfully transformed regular human skin cells into pluripotent stem cells. Spearheaded by Dr. Rajkumar…
Multi institutional team led by IIT Guwahati produces pluripotent stem cells from skin cells
Researchers from the Indian Institute of Technology Guwahati (IITG) in collaboration with scientists from Christian Medical College, Vellore, have reported a method to convert regular human skin cells into pluripotent stem cells. “The human body is made of many kinds of cells – nerve cells, heart cells, liver cells, pancreatic…
Sci-Hub | Association Between XRCC1 ARG399GLN and P53 ARG72PRO Polymorphisms and the Risk of Gastric and Colorectal Cancer in Turkish Population. Archives of Industrial Hygiene and Toxicology, 62(3)
Sci-Hub | Association Between XRCC1 ARG399GLN and P53 ARG72PRO Polymorphisms and the Risk of Gastric and Colorectal Cancer in Turkish Population. Archives of Industrial Hygiene and Toxicology, 62(3) | 10.2478/10004-1254-62-2011-2098 ◂ ↓ save Engin, A., Karahalil, B., Karakaya, A., & Engin, A. (2011). Association Between XRCC1 ARG399GLN and P53 ARG72PRO…
Characterizing the role of Phlda3 in the development of acute toxicity and malignant transformation of hematopoietic cells induced by total-body irradiation in mice
Mouse strains and procedures All animal procedures for this study were approved by the Institutional Animal Care and Use Committee (IACUC) at Duke University (protocol number A215-20-11 was approved on November 9, 2020). All methods were performed in accordance with the relevant guidelines and regulations. The study is reported in…
Low-dose radiation induces unstable gene expression in developing human iPSC-derived retinal ganglion organoids
RGCs from human iPSCs for genomic analysis We developed neuronal organoids, including RGCs from human iPSCs, to assess the effects of low-dose irradiation. Phase-contrast microscopy (Supple Fig. S1a) indicated time-dependent morphological changes in the embryonal body formed from human iPSCs, which corresponded to previous reports14, 15. Retinal development was evaluated…
We know almost nothing about thousands of proteins in the human body
Around 20,000 genes that code for proteins have been identified in humans, but the function of many of these proteins is unknown SCIENCE PHOTO LIBRARY A database of proteins, dubbed the “unknome”, that ranks proteins according to how much we have learned about them has revealed that we still know…
Strategies to therapeutically modulate cytokine action
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Pharmacological targeting of netrin-1 inhibits EMT in cancer
Ye, X. & Weinberg, R. A. Epithelial-mesenchymal plasticity: a central regulator of cancer progression. Trends Cell Biol. 25, 675–686 (2015). Article CAS PubMed PubMed Central Google Scholar Shibue, T. & Weinberg, R. A. EMT, CSCs, and drug resistance: the mechanistic link and clinical implications. Nat. Rev. Clin. Oncol. 14, 611–629…
The top biotech books of all time every science lover should read
It’s summertime and what’s better than to curl up in bed, or under a shady tree, or by the pool, and read a good book? We have curated a list of some of the best biotech books that are bound to get you hooked. From science fiction thrillers to eye-opening…
ncRNA | Free Full-Text | Effects of Controlled Ozone Exposure on Circulating microRNAs and Vascular and Coagulation Biomarkers: A Mediation Analysis
1. Introduction Tropospheric ozone (O3) is a ubiquitous, highly reactive oxidant pollutant produced by photochemical reactions between nitrogen oxides and volatile organic compounds in the presence of ultraviolet light [1]. Ambient O3 levels are regulated by the U.S. Environmental Protection Agency under the Clean Air Act, with the current National…
ncRNA | Free Full-Text | Long Non-Coding RNA Signatures in Lymphopoiesis and Lymphoid Malignancies
The most common types of B-cell lymphomas include chronic lymphocytic leukemia (CLL), mantle-cell lymphoma (MCL), Burkitt’s lymphoma (BL), follicular lymphoma (FL), and diffuse large B-cell lymphoma (DLBCL). Several studies have identified differently expressed lncRNAs in B-cell NHLs by using datasets that confirm the findings in tissue samples or cell lines…
DNA Sequencing: Another Weapon Against Cancer
Gene mutations and dysregulation of cellular pathways lead to the creation of cancer cells and potentially to tumour formation. Usually, the genes involved in tumour formation are oncogenes and tumour suppressor genes. Most oncogenes begin as proto-oncogenes. Proto-oncogenes are normally regulating cell proliferation, growth and differentiation and they control cell…
Annual Clinical Update on MDS Sees Hope in Precision Medicine, Use of Oral Therapy
The Annual Clinical Update in Hematological Malignancies, appearing online in early June and now publishing in the August issue of the American Journal of Hematology (AJH), includes an update on myelodysplastic syndromes by Guillermo Garcia-Manero, MD, who leads the MDS Section in the Department of Leukemia at The University of…
Moringa oleifera leaves extract ameliorates cardiotoxicity
Cyntia Gracesella Patintingan,1 Melva Louisa,2 Vetnizah Juniantito,3 Wawaimuli Arozal,2 Silmi Hanifah,1 Septelia Inawati Wanandi,4 Rajarajan Thandavarayan5 1Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 2Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 3Department of Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Agriculture Institute of Bogor,…
In Vivo Genome Editing of Stem Cells Induced by LNP-based Delivery of mRNA
Part of the job as a pediatric hematologist for Michael P. Triebwasser, MD, PhD, is to take care of patients during their bone marrow transplantation. Just last week, he took care of a patient with a severe combined immunodeficiency (SCID) disorder and another with a bone marrow disorder. Even though…
XPORT-EC-042 Studies Selinexor Maintenance in TP53WT Endometrial Cancer
About the Phase 3 XPORT-EC-042 Study Trial Name: A Phase 3, Randomized, Placebo-Controlled, Double-Blind, Multicenter Trial of Selinexor in Maintenance Therapy After Systemic Therapy for Patients With p53 Wild-Type, Advanced or Recurrent Endometrial Carcinoma ClinicalTrials.gov Identifier: NCT05611931 Sponsor: Karyopharm Therapeutics Inc. Recruitment Contact: Karyopharm Medical Information, (888) 209-9326, clinicaltrials@karyopharm.com Completion…
Rewiring cancer drivers to activate apoptosis
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Dynamic RNA profiles in the small intestinal epithelia of cats after Toxoplasma gondii infection | Infectious Diseases of Poverty
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Loss of Nudt15 thiopurine detoxification increases direct DNA damage in hematopoietic stem cells
Karran, P. & Attard, N. Thiopurines in current medical practice: Molecular mechanisms and contributions to therapy-related cancer. Nat. Rev. Cancer 8, 24–36. doi.org/10.1038/nrc2292 (2008). Article CAS PubMed Google Scholar Toksvang, L. N., Lee, S. H. R., Yang, J. J. & Schmiegelow, K. Maintenance therapy for acute lymphoblastic leukemia: Basic science…
WD repeat domain 82 (Wdr82) facilitates mouse iPSCs generation by interfering mitochondrial oxidative phosphorylation and glycolysis
Austenaa LMI, Barozzi I, Simonatto M, Masella S, Chiara GD, Ghisletti S, Curina A, Wit ED, Bouwman BAM, Pretis SD, Piccolo V, Termanini A, Prosperini E, Pelizzola M, Laat WD, Natoli G (2015) Transcription of mammalian cis-regulatory elements is restrained by actively enforced early termination. Mol Cell 60(3):460–474 Article CAS …
Solved please help me with these 3 questions, wither
Transcribed image text: Expandable Megakaryocyte Cell Lines Enable Clinically Applicable Generation of Platelets from Human Induced Pluripotent Stem Cells Sou Nakamura, 1 Naoya Takayama, 1 Shinji Hirata, 1 Hideya Seo, 1 Hiroshi Endo, 1 Kiyosumi Ochi, 1 Ken-ichi Fujita, 1 Tomo Koike, 1 Ken-ichi Harimoto, 1 Takeaki Dohda, 1 Akira…
ASCO Reading Room | PCR Assay IDs Pathologic Variants in Endometrial Cancer, With Advantages Over NGS
A quantitative genotyping polymerase chain reaction (PCR) assay is a quick, simple, and reliable alternative to DNA sequencing in endometrial cancer, researchers reported. Molecular classification is essential for prognosis and selection of the most effective treatment for women with endometrial cancer. The introduction of the endometrial cancer molecular classification algorithm,…
The complementarity of DDR, nucleic acids and anti-tumour immunity
Decout, A., Katz, J. D., Venkatraman, S. & Ablasser, A. The cGAS–STING pathway as a therapeutic target in inflammatory diseases. Nat. Rev. Immunol. 21, 548–569 (2021). Article CAS PubMed PubMed Central Google Scholar Martincorena, I. & Campbell, P. J. Somatic mutation in cancer and normal cells. Science 349, 1483–1489 (2015)….
Post-Doctoral Position job with Rutgers, The State Univ RBHS
A postdoctoral position supported by a NIH R01 grant is available for a highly motivated individual in the group of Dr. Wenwei Hu at Rutgers Cancer Institute of New Jersey/Rutgers University. The research interest of our lab is to study the development and progression of cancer, including cancer metastasis, cancer…