Tag: pbs

A Method to Obtain CTCs from Murine Colorectal Cancer Model

– Circulating Tumor Cells, or CTCs, are the cancer cells that stem from the primary tumor and enter the blood circulation. Most CTCs can survive in the bloodstream without attaching to the surface; therefore, we can detect them directly from the blood of most cancer patients. CTCs in the blood…

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Preparing Slides of CVS-11 Virus Antigen for Detecting Rabies-Specific Antibodies

To begin, wear personal protective equipment, including eye protection, a surgical mask, and non latex gloves. Prepare 20 milliliters of mouse neuroblastoma or BHK-21 cells to a concentration of 3.0 times 10 to the fifth cells per milliliter in EGM. Keep the prepared cells cold until they are ready to…

Continue Reading Preparing Slides of CVS-11 Virus Antigen for Detecting Rabies-Specific Antibodies

Indirect Fluorescent Antibody (IFA) Test to Detect Rabies-Specific Antibody Isotypes in Sera or Cerebral Spinal Fluid

To begin, prepare dilution to the patient’s serum or cerebral spinal fluid samples for testing. Prepare the required conjugate at the appropriate working concentration by diluting it in PBS with 0.05%Evans Blue. For indirect fluorescent antibody tests, retrieve the appropriate number of prepared antigen slides required for the assay. Allow…

Continue Reading Indirect Fluorescent Antibody (IFA) Test to Detect Rabies-Specific Antibody Isotypes in Sera or Cerebral Spinal Fluid

Endogenous Coriobacteriaceae enriched by a high-fat diet promotes colorectal tumorigenesis through the CPT1A-ERK axis

Bacteria Strain Cori.ST1911 was isolated from fresh stool of 20-week-old C57/BL6J mice fed a HFD at the Animal Center of the West China Hospital of Sichuan University. Briefly, faecal particles were ground with a glass grinding rod and suspended in sterile phosphate-buffered saline (PBS). After gradient dilution, the suspension was…

Continue Reading Endogenous Coriobacteriaceae enriched by a high-fat diet promotes colorectal tumorigenesis through the CPT1A-ERK axis

Isolation and Digestion of Mouse Carotid Artery for Single-Cell Suspension Preparation

To begin, spray the euthanized mouse with 75%alcohol. Then you sterilized dissecting scissors to open the skin and expose the chest cavity. Cut the diaphragm, then continue cutting upward toward the lower jaw and carefully remove excess connective tissues and fat exposing the carotid artery. Next, cut the inferior vena…

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Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2

Reagents and peptides CXC motif chemokine 14 (CXCL14) was purchased from BIOZOL (Eching, Germany). Proadrenomedullin N-terminal 20 peptide (PAMP-20) was obtained from Tocris Bioscience (Bristol, UK), and cortistatin-14 was from GenScript (NJ, USA). Custom synthesis of CXCL14 analogs was performed by JPT Peptide Technologies (Berlin, Germany) (see Supplementary Fig. 6). Stock…

Continue Reading Proinflammatory chemokine CXCL14 activates MAS-related G protein-coupled receptor MRGPRX2 and its putative mouse ortholog MRGPRB2

Rolling circle amplification assisted CRISPR/Cas12a dual-cleavage photoelectrochemical biosensor for highly sensitive detection of miRNA-21

Background: MicroRNA-21 has been determined to be the only microRNA overexpressed in 11 types of solid tumors, making it an excellent candidate as a biomarker for disease diagnosis and therapy. Photoelectrochemical (PEC) biosensors have been widely used for quantification of microRNA-21. However, most PEC biosensing processes still suffer from some…

Continue Reading Rolling circle amplification assisted CRISPR/Cas12a dual-cleavage photoelectrochemical biosensor for highly sensitive detection of miRNA-21

Domestic pigs are susceptible to experimental infection with non-human primate-derived Reston virus without the need for adaptation

Ethics and animal welfare statement All infectious work with RESTV, including sample inactivation, was performed in the Containment Level 4 laboratory (CL4) in accordance with the policies and protocols outlined by the Canadian Science Centre for Human and Animal Health Institutional Biosafety Committee. All animal work was performed in strict…

Continue Reading Domestic pigs are susceptible to experimental infection with non-human primate-derived Reston virus without the need for adaptation

Double Stranded DNA (dsDNA) (Nuclear Marker) – CloneID – DSD/958

Double Stranded DNA (dsDNA) (Nuclear Marker) – CloneID – DSD/958 | Scientist.com Skip to Main Content Go to Main Navigation Details Gene ID: Not Applicable Uniprot: Not Applicable Gene Name: Not Applicable Applications: IHC Species Reactivity: Human Clonality: Monoclonal Host: Mouse Clone ID: DSD/958 Protein Target: Not Applicable Supplied As:…

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Anti-EpCAM (Extracellular region) Antibody | Scientist.com

Anti-EpCAM (Extracellular region) Antibody | Scientist.com Skip to Main Content Go to Main Navigation Form: Protein G Purified Gene: EPCAM Host: Mouse Rrid: AB_3068418 Type: Primary Antibody Clone: M039 Buffer: PBS + 1 mg/ml BSA, 0.05% NaN3 and 50% glycerol Target: EpCAM (Extracellular region) Isotype: IgG1 Storage: Storage at -20°C…

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SMAD4 (Pancreatic Adenocarcinoma Marker) – CloneID – rSMAD4/6310

SMAD4 (Pancreatic Adenocarcinoma Marker) – CloneID – rSMAD4/6310 | Scientist.com Skip to Main Content Go to Main Navigation Details Gene ID: 4089 Uniprot: Q13485 Gene Name: SMAD4 Applications: IHC Species Reactivity: Human, mouse, , rat Clonality: Monoclonal Host: Recombinant Mouse Clone ID: rSMAD4/6310 Protein Target: SMAD4 Supplied As: 200ug/ml of…

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ALDH2 mitigates LPS-induced cardiac dysfunction, inflammation, and apoptosis through the cGAS/STING pathway | Molecular Medicine

Reagents and antibodies The details of the drugs and related reagents used in this study are presented in Additional file 1: Table S1. Animals A total of 48 male C57BL/6 mice were purchased from Byrness Weil Biotech Ltd. (License number: SCXK (Xiang) 2019-0004). The mice were of SPF-grade and had…

Continue Reading ALDH2 mitigates LPS-induced cardiac dysfunction, inflammation, and apoptosis through the cGAS/STING pathway | Molecular Medicine

csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axis | Parasites & Vectors

Human tissue samples The preoperative diagnosis of CS mainly relies on stool microscopy, enzyme-linked immunosorbent assay, or endoscopy. Fresh liver and intrahepatic bile duct specimens were collected from ICC patients with (CS-ICC group) and without (NC-ICC group) CS infection during partial hepatectomy at the First Hospital of Jilin University. Tumor tissues…

Continue Reading csi-miR-96-5p delivered by Clonorchis sinensis extracellular vesicles promotes intrahepatic cholangiocarcinoma proliferation and migration via the ferroptosis-related PTEN/SLC7A11/GPX4 axis | Parasites & Vectors

Apoptosis-resistant megakaryocytes produce large and hyperreactive platelets in response to radiation injury | Military Medical Research

Animals C57BL/6-Tg (Pf4-icre) Q3Rsko/J (Pf4-Cre) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). B6;129-Tmem173tm1(flox)Smoc (Stingfl) mice were purchased from Shanghai Model Organisms Center, Inc. (Shanghai, China). C57BL/6J-Ifnar1em1(flox)Cya (Ifnar1fl) mice were purchased from Cyagen Biosciences (Guangzhou, China). Stingcko and Ifnar1cko mice were generated by crossing Pf4-Cre mice with…

Continue Reading Apoptosis-resistant megakaryocytes produce large and hyperreactive platelets in response to radiation injury | Military Medical Research

Analysis of off-tumour toxicities of T-cell-engaging bispecific antibodies via donor-matched intestinal organoids and tumouroids

Human tissue samples Tissue samples and annotated data were obtained, and experimental procedures were performed within the framework of the non-profit foundation HTCR, including informed patient consent. Intestinal organoid and tumouroid cell culture Supplementary Table 1 provides anonymized basic donor information. After isolation of intestinal crypts following a previously described…

Continue Reading Analysis of off-tumour toxicities of T-cell-engaging bispecific antibodies via donor-matched intestinal organoids and tumouroids

Evidence of an intracellular creatine-sensing mechanism that modulates creatine biosynthesis via AGAT expression in human HAP1 cells

Cell lines and reagents HAP1 cells used to generate the reporter cells were grown from a frozen stock of HAP1 (C859) passage 6 purchased from Horizon Discovery LTD (Cambridge, UK). Iscove’s media (Ref: 098–150, Wisent) supplemented with 10% Fetal Bovine serum (Multicell) was used to grow HAP1 cells. All cells…

Continue Reading Evidence of an intracellular creatine-sensing mechanism that modulates creatine biosynthesis via AGAT expression in human HAP1 cells

Gadolinium Oxide Nanoparticles reinforce immune response

Boyi Yu,1– 4 Xuanyi Lu,5 Xianglong Feng,1– 4 Ting Zhao,1– 4 Jiaxin Li,1– 4 Yudie Lu,5 Fei Ye,1– 4 Xiongxiong Liu,1– 4 Xiaogang Zheng,1– 4 Zheyu Shen,5 Xiaodong Jin,1– 4 Weiqiang Chen,1– 4 Qiang Li1– 4 1Biomedical Center, Institute of Modern Physics, Chinese Academy of Sciences, Lanzhou, People’s Republic of…

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A transcriptomic taxonomy of mouse brain-wide spinal projecting neurons

Animals All experimental procedures were performed in compliance with animal protocols approved by the Institutional Animal Care and Use Committee at Boston Children’s Hospital (Protocol no. 20-05-4165 R). Mice were provided with food and water ad libitum, housed on a 12-hour light/dark schedule (7 a.m.–7 p.m. light period) with no more than five mice…

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A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain

Mouse breeding and husbandry All experimental procedures related to the use of mice were approved by the Institutional Animal Care and Use Committee of the AIBS, in accordance with NIH guidelines. Mice were housed in a room with temperature (21–22 °C) and humidity (40–51%) control within the vivarium of the AIBS…

Continue Reading A high-resolution transcriptomic and spatial atlas of cell types in the whole mouse brain

Bioactive glycans in a microbiome-directed food for children with malnutrition

Collection and handling of biospecimens obtained from participants in the randomized controlled clinical study of the efficacy of MDCF-2 The human study entitled ‘Community-based clinical trial with microbiota-directed complementary foods (MDCFs) made of locally available food ingredients for the management of children with primary moderate acute malnutrition (MAM)’ was approved…

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Elevated peripheral levels of receptor-interacting protein kinase 1 (RIPK1) and IL-8 as biomarkers of human amyotrophic lateral sclerosis

Ethics statement All the animal experiments were performed in accordance with the Guide for the Care and Use of Laboratory Animals and were approved by the Animal Care Committee of Three Gorges University, China (2019060B). The approval of primidone off-label prescription for ALS patients was obtained from the Ethics Committee…

Continue Reading Elevated peripheral levels of receptor-interacting protein kinase 1 (RIPK1) and IL-8 as biomarkers of human amyotrophic lateral sclerosis

Human RAD52 stimulates the RAD51-mediated homology search

Introduction Homologous recombination (HR) is an evolutionarily conserved process that plays a pivotal role in genome stability, diversity, and plasticity. HR is indeed a key repair pathway able to faithfully repair DNA damages including double-strand breaks (DSBs) and DNA gaps by copying the error-free information from the template DNA normally…

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12A-PE-100ul | Caspase 14, CT (Caspase-14, CASP-14, CASP14, EC 3)

Cat# C2089-12A-PE-100ul Size : 100ul Brand : US Biological Host rabbit Conjugate PE Isotype IgG Grade Affinity Purified Applications WB Shipping Temp Blue Ice Storage Temp 4°C Do Not Freeze Apoptosis-related Cysteine Protease, Caspase 14 Precursor Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western…

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Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2

Study design, ethical statement, and sample collection The vaccinated participants were recruited under the COVID-19 PROTECT study (2012/00917). The convalescent plasma samples were collected from subjects who were diagnosed with COVID-19 by positive polymerase chain reaction (PCR) results (study 2020/00120). All participants provided written informed consent in accordance with the…

Continue Reading Employment of a high throughput functional assay to define the critical factors that influence vaccine induced cross-variant neutralizing antibodies for SARS-CoV-2

A naturally occurring polyacetylene isolated from carrots promotes health and delays signatures of aging

Cell culture conditions Unless otherwise stated, cells were cultured with DMEM (Sigma Aldrich; #D6429) and 4.5 g/l glucose, phenol red, 10% FBS, and 1% penicillin/streptomycin with pH 7.4 and were incubated at 37 °C, 5% CO2, and 95% relative humidity. Wild-type HepG2 (#300198), HEK293 (#300192), and MCF-7 (#300273) were derived from CLS…

Continue Reading A naturally occurring polyacetylene isolated from carrots promotes health and delays signatures of aging

Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition

Patients, tumour Implantation, PDX expansion and characterization The study was conducted according to the Declaration of Helsinki. Tumours were collected from EC patients following Human Research Ethics Committee (HREC) approval (HREC/15/MHS/127) and QUT HREC (#1500000169, 1500000323). All participants provided written informed consent that their excess tissue could be used for…

Continue Reading Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition

Multi-factor designs in DiffBind

Multi-factor designs in DiffBind 1 Dears, I’m trying to analyze some ChIPseq data using DiffBind. Samples has been processed in two different times SampleID Name Factor IP Condition Replicates 5 PBS_Pol2 S3 Batch1 Pol2 PBS 1 6 PBS_Pol2 S9 Batch2 Pol2 PBS 2 7 C26_Pol2 S4 Batch1 Pol2 C26 1…

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Integrative taxonomy of Metastrongylus spp. in wild boars from Brazil | Parasites & Vectors

Study areas The samples were collected from wild boars hunted in rural properties from the municipalities of São Simão, Monte Azul, Paraíso, Colina, Matão, Bebedouro e Monte Alto (São Paulo), Ipiranga (Paraná), and Santo Antônio das Missões (Rio Grande do Sul) (Fig. 1). Fig. 1 Sampling collection sites of wild boars…

Continue Reading Integrative taxonomy of Metastrongylus spp. in wild boars from Brazil | Parasites & Vectors

Anti-Unconventional myosin-X MYO10 Antibody Clinisciences

Product Name Anti-Unconventional myosin-X MYO10 Antibody View all myosin X Antibodies SKU/Catalog Number A03647 Size 100ul Form Liquid Description Boster Bio Anti-Unconventional myosin-X MYO10 Antibody catalog # A03647. Tested in IHC, WB applications. This antibody reacts with Human, Mouse. Storage & Handling Store at -20°C for one year. For short…

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Survey of the infant male urobiome and genomic analysis of Actinotignum spp.

Wolfe, A. J. et al. Evidence of uncultivated bacteria in the adult female bladder. J. Clin. Microbiol. 50, 1376–1383 (2012). Article  PubMed  PubMed Central  Google Scholar  Hilt, E. E. et al. Urine is not sterile: Use of enhanced urine culture techniques to detect resident bacterial flora in the adult female…

Continue Reading Survey of the infant male urobiome and genomic analysis of Actinotignum spp.

Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease

mitoARCUS localizes to the mitochondrial matrix To evaluate the ability of mitoARCUS to specifically cleave m.3243G mutant mtDNA, cell lines containing varying levels of the mutation were generated. All of these cell lines were isolated from the same parental m.3243A>G cybrid cell line. Levels of the m.3243G mutation of each…

Continue Reading Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease

IJMS | Free Full-Text | Down-Regulation of Rice Glutelin by CRISPR-Cas9 Gene Editing Decreases Carbohydrate Content and Grain Weight and Modulates Synthesis of Seed Storage Proteins during Seed Maturation

3.1. Atypical Morphology of Protein Bodies in the Endosperm of Seeds from Glutelin Gene-Edited Transgenic Rice ER-derived PB-I vacuoles begin to form in response to the aggregation of β-/γ-zeins (maize) and 10-kDa prolamins (rice) and then expand by co-aggregation of α-/δ-zeins (maize) and 13-/16-kDa prolamins (rice) [3,22,23]. Saito’s group reported…

Continue Reading IJMS | Free Full-Text | Down-Regulation of Rice Glutelin by CRISPR-Cas9 Gene Editing Decreases Carbohydrate Content and Grain Weight and Modulates Synthesis of Seed Storage Proteins during Seed Maturation

Genome editing approaches with CRISPR/Cas9: the association of NOX4 expression in breast cancer patients and effectiveness evaluation of different strategies of CRISPR/Cas9 to knockout Nox4 in cancer cells | BMC Cancer

A. Bioinformatics analyzes Search strategy and study selection From April to June 2021, we read relevant and related publications in Medline, EMBASE, Web of Science, and Wanfang. The search term “NOX4” have been used. The articles that fit specified criteria were all added at once: (1) Participants were split into…

Continue Reading Genome editing approaches with CRISPR/Cas9: the association of NOX4 expression in breast cancer patients and effectiveness evaluation of different strategies of CRISPR/Cas9 to knockout Nox4 in cancer cells | BMC Cancer

How DNA Profiling Works

The term “DNA,” once used only by scientists, has become part of our everyday lexicon. It’s almost impossible to not know of DNA profiling, from the court system to genealogy. It’s also nearly impossible to be unaware of the controversy. Now that we can each have a profile that identifies…

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Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cells

hiPSC cultures and cardiomyocyte differentiation The hiPSC lines CBRCULi001-A54 and CBRCULi008-A55 were generated from a 44-year-old male and 75-year-old female control lymphoblastoids, respectively, and they were reprogramed at the LOEX core facility (Quebec City, QC, Canada). All the work with hiPSCs were approved by CIUSSS de la Capitale-Nationale ethics committee (Project…

Continue Reading Biophysical properties of NaV1.5 channels from atrial-like and ventricular-like cardiomyocytes derived from human induced pluripotent stem cells

Decline of DNA damage response along with myogenic differentiation

Introduction Proper functioning of all living organisms depends on the faithful maintenance and transmission of genomic information stored in the molecule of DNA. However, DNA integrity is continuously challenged by a variety of endogenous and exogenous agents causing DNA lesions which have a critical impact on cellular activities and homeostasis….

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The Mla system of diderm Firmicute Veillonella parvula reveals an ancestral transenvelope bridge for phospholipid trafficking

Bacterial strains and growth conditions Veillonella parvula SKV38 was grown in SK medium (10 g/L tryptone [Difco], 10 g/L yeast extract [Difco], 0.4 g/L disodium phosphate, 2 g/L sodium chloride, and 10 ml/L 60% [wt/vol] sodium DL-lactate; described in ref. 51. Cultures were incubated at 37 °C in anaerobic conditions, either in anaerobic bags (GENbag anaero;…

Continue Reading The Mla system of diderm Firmicute Veillonella parvula reveals an ancestral transenvelope bridge for phospholipid trafficking

31L-APC-100ul | Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha)

Cat# I7663-31L-APC-100ul Size : 100ul Brand : US Biological Host mouse Conjugate APC Isotype IgG1 Clone Number 10B1132 (IL-A111) Grade Affinity Purified Applications IHC IP Shipping Temp Blue Ice Storage Temp 4°C Do Not Freeze Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Functional Assays. Other applications not tested. Recommended…

Continue Reading 31L-APC-100ul | Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha)

31L1-100ug | Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha)

Cat# I7663-31L1-100ug Size : 100ug Brand : US Biological Host mouse Conjugate FITC Isotype IgG1 Clone Number 10B1132 (IL-A111) Grade Affinity Purified Applications FC Shipping Temp Blue Ice Storage Temp -20°C The CD25 cell surface antigen is a 55kDa glycoprotein also known as Interleukin-2 receptor alpha chain. Bovine CD25 is…

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Megakaryocytes possess a STING pathway that is transferred to platelets to potentiate activation

Introduction Host defence against infection relies on two important systems: the immune and the coagulation systems. Platelets are anucleate cells produced by megakaryocytes and are responsible for blood clotting and haemostasis (Lefrancais et al, 2017). They are also very abundant and play unexpected roles in immune responses (Maouia et al,…

Continue Reading Megakaryocytes possess a STING pathway that is transferred to platelets to potentiate activation

Elevated stress response marks deeply quiescent reserve cells of gastric chief cells

Generation of inducible H2b-GFP knock-in mice Generation of inducible H2b-GFP knock-in mice was performed by using CRISPR/Cas943. In brief, a mixture containing a sgRosa26-1 crRNA43 (8.7 ng/μl, Fasmac, Japan), a tracrRNA (14.3 ng/μl, Fasmac, Japan), a single strand oligo donor nucleotide (ssODN) composed of 5′ arm, adenovirus splicing acceptor, SV40 pA, TRE3G…

Continue Reading Elevated stress response marks deeply quiescent reserve cells of gastric chief cells

31L-AP-100ul | Interleukin 2 Receptor, alpha (IL-2Ra, IL-2R alpha)

Cat# I7663-31L-AP-100ul Size : 100ul Brand : US Biological Host mouse Conjugate AP Isotype IgG1 Clone Number 10B1132 (IL-A111) Grade Affinity Purified Applications IHC IP Shipping Temp Blue Ice Storage Temp 4°C Do Not Freeze Applications: Suitable for use in Immunohistochemistry, Immunoprecipitation and Functional Assays. Other applications not tested. Recommended…

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Iron derived from NCOA4-mediated ferritinophagy causes cellular senescence via the cGAS-STING pathway

Materials and reagents Tert-butyl hydroperoxide (TBH) were purchased from Sigma-Aldrich (St. Louis, USA). Ferric ammonium citrate (FAC) and D-galactose (D-gal) was obtained from Selleck (Shanghai, China) and Aladdin (Shanghai, China), respectively. Deferoxamine (DFO), Mito-TEMPO (Mito-T), 3-Methyladenine (3-MA), Chloroquine (CQ), Bafilomycin A1 (Baf-A1), and H-151 were purchased from MedChemExpress (NJ, USA)….

Continue Reading Iron derived from NCOA4-mediated ferritinophagy causes cellular senescence via the cGAS-STING pathway

11-100test | EpCAM antibody [VU-1D9] (PerCP-Cy5.5) Clinisciences

Application Note *Optimal dilutions/concentrations should be determined by the researcher. Application Recommended Dilution FACS 4 μl reagent / 100 μl of whole blood or 10⁶ cells in a suspension Not tested in other applications. Calculated MW 35 kDa. ( Note ) Product Note This antibody recognizes an epitope within EGF-like…

Continue Reading 11-100test | EpCAM antibody [VU-1D9] (PerCP-Cy5.5) Clinisciences

Monocyte Isolation and Monocyte Differentiation into Monocyte-Derived Dendritic Cells

Begin isolating peripheral blood mononuclear cells by accessing the human buffy coat and transferring seven milliliters into a sterile 15 milliliters conical tube. Add six milliliters of PBS solution for a preliminary wash. Centrifuge the tube for 10 minutes at 1, 100 G in a centrifuge with a swing rotor,…

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Maturation Profiling of Human Monocyte-Derived Dendritic Cells (mo-DCs)

Begin collecting a new sample of the cells of interest for antibody staining by washing the cells at room temperature for five minutes at 300 G with normal break. Distribute the cells into micro tubes. To perform the staining using the desired antibodies, incubate the fluorescence conjugated antibodies in the…

Continue Reading Maturation Profiling of Human Monocyte-Derived Dendritic Cells (mo-DCs)

IDG-SW3 Cell Culture in a Three-Dimensional Extracellular Matrix

Here, we present a protocol for culturing IDG-SW3 cells in a three-dimensional (3D) extracellular matrix. We detailed a protocol to establish a 3D system using an extracellular metrics along with collagen one for analyzing formation and gene expression through osteogenesis. Extracellular metrics can increase the transparency of the collagen one…

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STAR output

STAR output 1 Hello, I am trying to map with STAR but it is not clear to me why I am not getting the SAM/BAM mapping file, could you help me? [epola@mazorka alignment_STAR]$ ls -lh total 13M -rw-rw-r– 1 epola epola 13M Nov 17 12:34 SRR22164928SJ.out.tab -rw——- 1 epola epola…

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COL10A1 promotes tumorigenesis by modulating CD276 in pancreatic adenocarcinoma | BMC Gastroenterology

Bioinformatics analysis The GEO database (www.ncbi.nlm.nih.gov/geo/) provided detailed gene expression information for the GSE62165 microarray. Differentially expressed genes were identified using the online tool Morpheus (software.broadinstitute.org/morpheus/) (fold change = normal pancreatic tissue gene expression/PDAC tissue gene expression, P value < 0.05 and |log2(fold change)|>1). The downstream analysis was based on the genes with the…

Continue Reading COL10A1 promotes tumorigenesis by modulating CD276 in pancreatic adenocarcinoma | BMC Gastroenterology

DRIP-qPCR validation – Mendeley Data

Description 1. HEK293 cells were obtained from the National Collection of Authenticated Cell Culture of China (accession number: SCSP-5014) and cultured in DMEM supplemented with 10% FBS and penicillin/streptomycin (100U/ml) at 37°C with 5%CO2. Expression vector carrying NOTCH2NLC exon1 with different numbers of GGC repeats were prepared and transfected into…

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RhoA suppresses pseudorabies virus replication in vitro | Virology Journal

Materials The Cell Counting Kit-8 (CCK-8) was ordered from Yeasen BioTechnologies co, Ltd. (Shanghai, China). Anti-GAPDH was purchased from Proteintech Group, Inc. (Chicago, USA). Anti RhoA was purchased from Novus Biologicals (Colorado, USA). Antiserum against PRV glycoprotein gB was generated by immunization of mice with purified recombinant gB. Goat anti-Mouse…

Continue Reading RhoA suppresses pseudorabies virus replication in vitro | Virology Journal

Rapid purification and characterization of circulating small extracellular vesicles on a label-free lab-on-a-chip

Device layout and graphical outline of experimental flow. Small extracellular vesicles (sEVs) were simultaneously extracted from human biofluids using the insulator-based dielectrophoretic (iDEP) device, wherein a trapping zone was created at the tip region of micropipettes by balancing the dielectrophoretic (DEP) force with two electrokinetic forces, including electroosmosis (EOF) and…

Continue Reading Rapid purification and characterization of circulating small extracellular vesicles on a label-free lab-on-a-chip

Saponin treatment for eukaryotic DNA depletion alters the microbial DNA profiles by reducing the abundance of Gram-negative bacteria in metagenomics analyses

INTRODUCTION Microbiome research, especially the detection of microorganisms by molecular techniques, has become a fundamental tool for investigating host-associated bacteria, such as those harbored by veterinary or human clinical samples[1,2]. Next-generation sequencing (NGS) approaches now enable the identification of slow-growing, non-cultivable, or non-viable bacteria contained in clinical specimens without relying…

Continue Reading Saponin treatment for eukaryotic DNA depletion alters the microbial DNA profiles by reducing the abundance of Gram-negative bacteria in metagenomics analyses

PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation

Antibodies Antibodies used in the present study were as follows: rabbit anti-phospho-YAP (S127) (#13008, dilution 1:500), rabbit anti-phospho-LATS1 (S909) (#9157, dilution 1:500), rabbit anti-YAP (#14074, dilution 1:100 for immunofluorescence), and rabbit anti-PPP1R12A (#8574, dilution 1:1000 for western blot, 1:200 for immunofluorescence) from Cell Signaling Technology; rabbit anti-LATS1 (A300-477A, dilution 1:2000)…

Continue Reading PPP1R12A is a recycling endosomal phosphatase that facilitates YAP activation

ABCB1 and immune genes in breast cancer

Introduction Chemoresistance is a major challenge for breast cancer treatment.1 The mechanisms of chemoresistance are complex because of crosstalk between receptor tyrosine kinases and downstream pathways, deregulation of cell-cycle and apoptosis regulators, and modulation of tumor-infiltrating immune cells.2 The ATP-binding cassette (ABC) superfamily is one of the largest families of…

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A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of non-coding elements in vivo

Animal models All procedures involving animals were approved by the Institutional Animal Care and Use Committee (IACUC) at Washington University in St. Louis, MO. Veterinary care and housing was provided by the veterinarians and veterinary technicians of Washington University School of Medicine under Dougherty lab’s approved IACUC protocol. All protocols…

Continue Reading A Cre-dependent massively parallel reporter assay allows for cell-type specific assessment of the functional effects of non-coding elements in vivo

Myristoylated, alanine-rich C-kinase substrate (MARCKS) regulates toll-like receptor 4 signaling in macrophages

Cell line and LPS stimulation conditions Immortalized mouse macrophages (IMM), including the IMMs from TLR4 -/- mouse (generated by Bruce Beutler’s laboratory47) and available from Jackson Laboratory) were a generous gift from Dr. Eicke Latz48,49. The cells were cultured in complete DMEM (Gibco, Grand Island, NY, USA) complemented with 10%…

Continue Reading Myristoylated, alanine-rich C-kinase substrate (MARCKS) regulates toll-like receptor 4 signaling in macrophages

Ginsenoside Rg3 enriches SCFA-producing commensal bacteria to confer protection against enteric viral infection via the cGAS-STING-type I IFN axis

Rg3 elicits protection against local and systemic enteric virus infection by regulating the intestinal microbiome To assess the antiviral effects of Rg3 in vivo, groups of C57BL/6J mice (referred to as WT B6 hereafter) were orally administered Rg3 for 3 consecutive days, inoculated with MNV-1 by the peroral route, and…

Continue Reading Ginsenoside Rg3 enriches SCFA-producing commensal bacteria to confer protection against enteric viral infection via the cGAS-STING-type I IFN axis

Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

Start by connecting the tubing of the microperfusion apparatus to the chip holder. Flush the line with a baseline secretagogue, like two-millimolar glucose, for five minutes. Aspirate any extra fluid from the top and bottom valves of the microchip. The top half of the microchip ensures adequate sealing of wells,…

Continue Reading Synchronous Assessment of Fluorescent Biosensor Dynamics and Hormone Profiles in Human Pseudoislets

Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2

Ethic statement The Clinical Research Ethics Review Committee of Kyushu University Hospital approved the study (permit no. 29-43, 30-268, and 700-00). Written informed consent for the current research project was obtained before the tumor tissue, and a blood sample were harvested. This study was also conducted according to the principles…

Continue Reading Biological and genetic characterization of a newly established human external auditory canal carcinoma cell line, SCEACono2

r – ggplot x-axis ticks for pcc outcome – need symbols and scale_x_discrete not working

I’ve run a sensitivity analysis using the LHS-PRCC technique for 16 parameters and 10,000 different parameter sets. The outcome is a large pcc. (I’ll give a generic example using 100) df <- cbind( pcb = runif(100,0,1), pbc = runif(100,0,1), psb = runif(100,0,1), pbs = runif(100,0,1), pci = runif(100,0,1), pic =…

Continue Reading r – ggplot x-axis ticks for pcc outcome – need symbols and scale_x_discrete not working

FAT10 is phosphorylated by IKK{beta} to inhibit the antiviral type-I interferon response

Introduction The innate immune system represents the host´s first-line defense against viral infections (Koyama et al, 2008). In this context, IFN-I are produced by all nucleated cells and represent the principal cytokines that counteract viral replication (Ivashkiv & Donlin, 2014). In fact, several receptors of the infected cell can recognize…

Continue Reading FAT10 is phosphorylated by IKK{beta} to inhibit the antiviral type-I interferon response

Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus

Transcription and RNA processing The occupancy \({n}_{{{{{{\rm{ps}}}}}}}\) of the TSS of the active rDNA by Pol I follows the kinetic equation $$\frac{d}{{dt}}{n}_{{{{{{\rm{ps}}}}}}}={k}_{{{{{{\rm{on}}}}}}}\rho \left(1-{n}_{{{{{{\rm{ps}}}}}}}\right)-{k}_{{{{{{\rm{off}}}}}}}{n}_{{{{{{\rm{ps}}}}}}}-{k}_{{{{{{\rm{e}}}}}}}{n}_{{{{{{\rm{ps}}}}}}}.$$ (5) Equation (5) suggests that the occupancy \({n}_{{{{{{\rm{ps}}}}}}}\) changes due to the binding of Pol I from the FC (the first term), the unbinding of Pol I…

Continue Reading Nascent ribosomal RNA act as surfactant that suppresses growth of fibrillar centers in nucleolus

Expression profiles of circRNAs and biomarkers for COPD

Introduction Chronic obstructive pulmonary disease (COPD) is a chronic inflammatory lung disease featured by airflow obstruction with high prevalence, morbidity, and mortality. It is reported that COPD is the third leading cause of death globally at present, and the incidence of COPD in adults over 40 years is more than…

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An FcRn-targeted mucosal vaccine against SARS-CoV-2 infection and transmission

Mice and Golden Syrian hamsters All research experiments in this study comply with all relevant ethical regulations. The Institutional Animal Care and Use Committee approved the animal protocol at the University of Maryland (#R-APR-20-18 and #R-MAR-21-19). The animals were acclimatized at the animal facility for 4–6 days before initiating experiments….

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The DNA-binding induced (de)AMPylation activity of a Coxiella burnetii Fic enzyme targets Histone H3

Statistics and reproducibility Anti-AMP IP for LC-MS/MS analysis was performed in three independent biological replicates (n = 3). TSA assay data represents technical triplicates. Time-resolved (de)AMPylation analyzed by LC-MS was performed as biological triplicates. CD measurements were performed in technical triplicates. Anisotropy data is shown as technical triplicates. Anisotropy measurements were repeated…

Continue Reading The DNA-binding induced (de)AMPylation activity of a Coxiella burnetii Fic enzyme targets Histone H3

Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection | Parasites & Vectors

CFAV The CFAV used in this study was derived from Ae. aegypti Aag2 cells cultured in Schneider’s Drosophila Medium (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum (Gibco, Thornton, Australia). The Aag2 cells were disrupted by three cycles of freeze-thawing and the supernatant was harvested as the CFAV stock…

Continue Reading Cell fusing agent virus isolated from Aag2 cells does not vertically transmit in Aedes aegypti via artificial infection | Parasites & Vectors

Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2

List of reagents Chloramine-T trihydrate (Merck, Darmstadt, Germany), Copper(II)Sulphate, Penicillin, Streptomycin, RPMI-1640 (Sigma-Aldrich, Burlington, MA, USA), RPMI-1640 (Euroclone, Pero, Italy), DMEM low glucose (Sigma-Aldrich), Trypsin–EDTA (Sigma-Aldrich), Blasticidin hydrochloride (Sigma-Aldrich), Trichloroacetic acid (Merck), Fetal Bovine Serum (Merck, Darmstadt, Germany), Iodine 125 Radionuclide (Perkin Elmer, Waltham, Mass., USA), Iodogen™ iodination reagent (Pierce,…

Continue Reading Highly oxidized albumin is cleared by liver sinusoidal endothelial cells via the receptors stabilin-1 and -2

The Atoh1-Cre Knock-In Allele Ectopically Labels a Subpopulation of Amacrine Cells and Bipolar Cells in Mouse Retina

Abstract The retina has diverse neuronal cell types derived from a common pool of retinal progenitors. Many molecular drivers, mostly transcription factors, have been identified to promote different cell fates. In Drosophila, atonal is required for specifying photoreceptors. In mice, there are two closely related atonal homologs, Atoh1 and Atoh7….

Continue Reading The Atoh1-Cre Knock-In Allele Ectopically Labels a Subpopulation of Amacrine Cells and Bipolar Cells in Mouse Retina

Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection

Ethics statement The animal experiments were approved by the Animal Ethics Committee of the Wuhan Institute of Virology, Chinese Academy of Sciences (approval number: WIVA21202104). All animal experiments involving NiV were executed in the animal biosafety level 4 (ABSL-4) facility at the National Biosafety Laboratory (Wuhan), Chinese Academy of Sciences….

Continue Reading Both chimpanzee adenovirus-vectored and DNA vaccines induced long-term immunity against Nipah virus infection

Anti-PspCas13b antibody [12F2-A10] (AB273440) | Abcam

Mouse Recombinant Monoclonal PspCas13b antibody. Validated in WB and tested in Transfected cell lysate – Prevotella pectinovora samples. Recombinant See all formulations & conjugations (1) Isotype IgG2a Host species Mouse Conjugation Unconjugated Storage buffer Preservative: 0.01% Sodium azide Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA Form Liquid Clonality…

Continue Reading Anti-PspCas13b antibody [12F2-A10] (AB273440) | Abcam

Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Presented here is a protocol to radiolabel cells with a positron emission tomography (PET) radioisotope, 89Zr (t1/2 78.4 h), using a ready-to-use radiolabeling synthon, [89Zr]Zr-p-isothiocyanatobenzyl-desferrioxamine ([89Zr]Zr-DBN). Radiolabeling cells with [89Zr]Zr-DBN allows noninvasive tracking and imaging of administered radiolabeled cells in the body with PET for up to 7 days post-administration….

Continue Reading Positron Emission Tomography Imaging of Cell Trafficking: A Method of Cell Radiolabeling

Production of leishmanin skin test antigen from Leishmania donovani for future reintroduction in the field

Study design and ethical statement All research complies with all relevant ethical regulations. Animal experiments in this study were reviewed and approved by the Animal Care and Use Committee of the Center for Biologics Evaluation and Research, U.S. Food and Drug Administration (ASP-1999#23 and ASP-1995#26) and the National Institute of…

Continue Reading Production of leishmanin skin test antigen from Leishmania donovani for future reintroduction in the field

Recombinant Full Length Rat Epithelial Cell Adhesion Molecule(Epcam) Protein, His-Tagged RFL3774RF

Source : E.coli expression system Species : Rattus norvegicus (Rat) Tag : His Form : Lyophilized powder Protein length : Full Length of Mature Protein (24-315) AA Sequence…

Continue Reading Recombinant Full Length Rat Epithelial Cell Adhesion Molecule(Epcam) Protein, His-Tagged RFL3774RF

Job submission on HPC using pbs script – User discussions

GROMACS version:2022.1GROMACS modification: YesRespected expert, I am using our institute HPC for mdrun. I can utilise one node but cannot utilise multiple nodes. I have learnt that PBS.sh script(image attached)is used for job submission on our institute’s HPC Even though i have tried modifications like varying number of nodes, process…

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CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane

Introduction Cystic fibrosis (CF) is a lethal, autosomal recessive disorder caused by mutations in the CFTR gene that results in aberrant transport of chloride, bicarbonate, and sodium ions (Kerem et al, 1989; Riordan et al, 1989; Rommens et al, 1989; Tabcharani et al, 1991; Poulsen et al, 1994; Stutts et…

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Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria

Isolation of phyllosphere isolates All bacterial strains were isolated in June 2021 from the flag leaves of four wheat cultivars (Sheriff, Heerup, Rembrandt and Kvium) grown in an experimental field in Høje Taastrup, near Copenhagen, Denmark. Wheat flag leaves were picked, pooled, and either washed or blended prior to dilution…

Continue Reading Widespread and largely unknown prophage activity, diversity, and function in two genera of wheat phyllosphere bacteria

Synergistic effects of Azithromycin and DMXAA

Kanoktip Petcharat,1 Narongsuk Munkong,2 Rungthip Thongboontho,1 Widsanusan Chartarrayawadee,3 Arthid Thim-Uam1 1Division of Biochemistry, School of Medical Sciences, University of Phayao, Phayao, 56000, Thailand; 2Department of Pathology, School of Medicine, University of Phayao, Phayao, 56000, Thailand; 3Division of Chemistry, School of Science, University of Phayao, Phayao, 56000, Thailand Correspondence: Arthid Thim-Uam,…

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Recombinant Human Retinoblastoma-Like 2 (RBL2), transfected HEK293 cell lysate

Recombinant Human Retinoblastoma-Like 2 (RBL2), transfected HEK293 cell lysate | RayBiotech The store will not work correctly in the case when cookies are disabled. JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on…

Continue Reading Recombinant Human Retinoblastoma-Like 2 (RBL2), transfected HEK293 cell lysate

Inferring bacterial transmission dynamics using deep sequencing genomic surveillance data

Study design Experiments were performed in accordance with the New Zealand Animal Welfare Act (1999) and institutional guidelines provided by the University of Auckland Animal Ethics Committee, which reviewed and approved these experiments under application R1003. We did not use any specific randomisation process to allocate animals to a particular…

Continue Reading Inferring bacterial transmission dynamics using deep sequencing genomic surveillance data

Kinetics of sealing for transient electropores in isolated mammalian skeletal muscle cells

文献主旨 Kinetics of sealing for transient electropores in isolated mammalian skeletal muscle cellsBioelectromagnetics 20:194 –201 (1999)Kinetics of Sealing for TransientElectropores in Isolated MammalianSkeletal Muscle CellsMartin Bier,* Stephanie M. Hammer, Daniel J. Canaday,and Raphael C. Lee*The Department of Organismal Biology and Anatomy, The Department of Surgery, Pritzker School of Medicine, The…

Continue Reading Kinetics of sealing for transient electropores in isolated mammalian skeletal muscle cells

mRNA vaccine encoding Gn provides protection against severe fever with thrombocytopenia syndrome virus in mice

mRNA preparation The DNA template for the mRNA vaccine comprised a DNA fragment encoding the SFTSV Gn protein. The DNA templates were cloned into a plasmid vector with backbone sequence elements (T7 promoter, 5′- and 3′-UTR, 100 nucleotide poly(A) tail) interrupted by a linker (A50LA50, 20 nucleotides) to improve RNA…

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Circulating DNA and frequency of colorectal cancer brain metastases in a presumed high-risk group

The study was approved by The Central Denmark Region Committees on Health Research Ethics (1-10-72-269-20), and it was prospectively registered with ClinicalTrials.gov (ClinicalTrials.gov identifier: NCT05185557). The research was carried out according to the principles set out in the Declaration of Helsinki 1964 and all subsequent revisions. Written and orally informed…

Continue Reading Circulating DNA and frequency of colorectal cancer brain metastases in a presumed high-risk group

Reduction of retinal ganglion cell death in mouse models of familial dysautonomia using AAV-mediated gene therapy and splicing modulators

Animals All mice were housed in the AALAC-accredited Animal Resource Center at Montana State University. All animal use protocols were approved by the Montana State University Institutional Animal Care and Use Committee (IACUC) (protocol No. 2020-15-IA; Bozeman, MT). The study fulfilled the ARRIVE guidelines, and all experiments complied with relevant…

Continue Reading Reduction of retinal ganglion cell death in mouse models of familial dysautonomia using AAV-mediated gene therapy and splicing modulators

Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia

. 2023 Dec;14(1):2244235. doi: 10.1080/21655979.2023.2244235. Affiliations Expand Affiliations 1 Bioprocess Engineering, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany. 2 Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany. 3 University of Natural Resources and Life Sciences, Vienna (BOKU), Department of Biotechnology (DBT), Institute of Bioprocess Science…

Continue Reading Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia

Orange juice containing Pediococcus acidilactici CE51 modulates the intestinal microbiota and reduces induced inflammation in a murine model of colitis

In view of the need for studies on compounds that act in the treatment and prevention of ulcerative colitis, this study evaluated and found the positive effect of the probiotic drink containing P. acidilactici CE51 regarding the reduction of inflammatory parameters presented in histopathological analyzes and modulation of the gut…

Continue Reading Orange juice containing Pediococcus acidilactici CE51 modulates the intestinal microbiota and reduces induced inflammation in a murine model of colitis

Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection

Biotechnol Rep (Amst). 2023 Dec; 40: e00814. Nusrat Tazin aDepartment of Electrical and Computer Engineering, University of Utah, Salt Lake City, UT, USA Christopher Jordon Lambert bDepartment of Mechanical Engineering, University of Utah, Salt Lake City, Utah, USA Raheel Samuel bDepartment of Mechanical Engineering, University of Utah, Salt Lake City,…

Continue Reading Transgenic expression in zebrafish embryos with an intact chorion by electroporation and microinjection

Ingenio Kit-Amaxa 100 rxn, 0.2 cm Cuvette/Droppers

Description High Efficiency Electroporation– Deliver to hard to transfect cell lines and primary cells Compatible with Most Conventional Electroporation Devices – Use your existing system including the Ingenio® EZporator®, Lonza-Amaxa®, Bio-Rad® or Harvard BTX® Save Money and Reduce Research Costs Without Sacrificing Performance – Ingenio® Electroporation Solution is available as a stand-alone solution or…

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Trinity Insilico Normalization

Trinity Insilico Normalization 1 Hello, I am trying to run trinity but on two samples but I am confused. I need help please. The process tells me this: perl: warning: Setting locale failed. perl: warning: Please check that your locale settings: LANGUAGE = (unset), LC_ALL = (unset), LANG=”C.UTF-8″ are supported…

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Production of Recombinant Adeno-Associated Viral Vectors and Transduction of Cultured Cells

After cloning the gene of interest in adeno-associated ITR containing plasmid, seed three times 10 to the fifth cells into a six-well plate using pre-warmed DMEM supplemented with 10%FBS. Allow the cells to grow at 37 degrees Celsius and 5%carbon dioxide to attain 75 to 90%confluence. Next, dissolve 100 milligrams…

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Identification of hub genes associated with gastric cancer

Introduction GC is a common malignant tumor of digestive system. According to the International Agency for Research on Cancer (IARC) of the World Health Organization, there were 19.29 million new cancer cases and 9.96 million deaths worldwide in 2020, of which the number of new cases of GC was 1089,103…

Continue Reading Identification of hub genes associated with gastric cancer

MICA: a multi-omics method to predict gene regulatory networks in early human embryos

Introduction After the fusion of the oocyte and sperm, the zygote undergoes a series of cell divisions until it forms a blastocyst before implantation into the uterus. A human blastocyst is formed of a fluid-filled cavity and ∼200 cells that comprise three distinct cell types: the trophectoderm (TE), which gives…

Continue Reading MICA: a multi-omics method to predict gene regulatory networks in early human embryos

Identification of CCZ1 as an essential lysosomal trafficking regulator in Marburg and Ebola virus infections

Cells and viruses Haploid mSCs AN3–12 are a feeder independent clonal derivative of HMSc2 isolated from mice oocyte and maintained at IMBA52. The AN3–12 library and knocked out cells used for the haploid screening was obtained from IMBA (Austria)14,52. AN3–12 cells were validated by STR analysis. Haploid mES cells were…

Continue Reading Identification of CCZ1 as an essential lysosomal trafficking regulator in Marburg and Ebola virus infections

How to reduce kspace timing% – LAMMPS Beginners

Roger October 25, 2023, 6:39am 1 Dear lammps users, Currently, I’m using LAMMPS version 16 Mar 2018, to run water droplet-silica simulation. I’m using 10 nodes with 40 cpus per node to run a 10nm x 10nm x 10nm system for 1 ns simulation. And the simulation can’t finish within…

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Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality

Experimental design Primary T cell isolation, expansion, and cell culture Primary T cells were isolated from human peripheral blood mononuclear cells (PBMCs) of healthy donors collected by the Institute for Transfusion Medicine of the University Clinic of Leipzig, Germany, approved by the Ethics Committee of the Faculty of Medicine of…

Continue Reading Comparison of two lab-scale protocols for enhanced mRNA-based CAR-T cell generation and functionality

How CRISPR Companies Target Sickle Cell Anemia

A Crippling And Painful Disease Sickle Cell Disease (SCD) is a blood disease caused by a genetic mutation. This mutation creates abnormal hemoglobin, the protein carrying oxygen in the blood’s red cells. As a result, red cells are shaped like sickles and tend to get stuck in blood vessels, causing…

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Passive diffusion accounts for the majority of intracellular nanovesicle transport

Introduction Trafficking of proteins, lipids, and other molecules between cellular compartments is carried out by vesicular carriers. Material destined for transfer is packaged into a small trafficking vesicle at the donor compartment; the vesicle must then travel to its destination, before fusing with the target compartment to deliver the material…

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Who is Mitochondrial Eve?

Our Mother, Our shared legacy Imagine a journey back in time to the dawn of humanity. You follow the footsteps of your ancestors, travelling across vast continents and treacherous seas. Along the way, you witness the birth of new cultures and the rise and fall of civilizations. Your journey finally…

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Recombinant Full Length Human Epithelial Cell Adhesion Molecule(Epcam) Protein, His-Tagged RFL5563HF

Source : E.coli expression system Species : Homo sapiens (Human) Tag : His Form : Lyophilized powder Protein length : Full Length of Mature Protein (24-314) AA Sequence…

Continue Reading Recombinant Full Length Human Epithelial Cell Adhesion Molecule(Epcam) Protein, His-Tagged RFL5563HF

Antiviral type III CRISPR signalling via conjugation of ATP and SAM

Cloning Supplementary Table 1 shows the synthetic gene, DNA and RNA oligonucleotide sequences used in this study. The synthetic genes encoding B.fragilis Cas6, CorA, NrN and C. botulinum SAM lyase purchased as g-blocks (IDT) were codon-optimized for expression in E. coli C43 (DE3) via the vector pEhisV5TEV, which encodes eight…

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