Tag: pbs

Pacbio job searching

Chula Vista scolds Republic Service for lingering strike, may sever ties with waste hauler. Public Safety. County Board of Supervisors approves ordinance to outlaw ghost guns. San Diego City Council approves school rebuild plan. New proposal would transform much of northeast Mission Bay into marshland. Two people shot, deputies searching…

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Development of a mammalian cell-based ZZ display system for IgG quantification | BMC Biotechnology

Cell culture CHO cells (a kind gift provided by Prof. Tom K. Hei, Columbia University, USA) were cultured in DMEM/F12 medium (Gibco, USA) containing 10% fetal bovine serum (FBS, Bio-west, USA), 100 U/mL penicillin and 100 µg/mL streptomycin (Beyotime, China), at 37 °C in a 5% CO2 incubator. Vector construction To capture…

Continue Reading Development of a mammalian cell-based ZZ display system for IgG quantification | BMC Biotechnology

Episomal Reprogramming of Dermal Fibroblasts to Generate Human-Induced Pluripotent Stem Cells (hiPSCs)

To begin, take a flask of cultured fibroblast cells, coat a 10-centimeter cell culture plate suitable for hiPSC culture with four milliliters of cold coating solution per plate, and incubate the plate for one hour at 37 degrees Celsius. Afterward, remove the media from the fibroblasts and wash them with…

Continue Reading Episomal Reprogramming of Dermal Fibroblasts to Generate Human-Induced Pluripotent Stem Cells (hiPSCs)

Selection, Expansion, and Quality Control of hiPSCs Generated from Dermal Fibroblasts

Transcript To begin, take the plate of electroporated fibroblasts and observe the formation of hiPSC colonies under a 10x to 20x objective phase contrast microscope. The hiPSC colonies with 300 micrometer diameter, distinct borders, and a high nuclei to body ratio are suitable for picking. Before picking, coat the wells…

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Moringa oleifera leaves extract ameliorates cardiotoxicity

Cyntia Gracesella Patintingan,1 Melva Louisa,2 Vetnizah Juniantito,3 Wawaimuli Arozal,2 Silmi Hanifah,1 Septelia Inawati Wanandi,4 Rajarajan Thandavarayan5 1Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 2Department of Pharmacology and Therapeutics, Faculty of Medicine, Universitas Indonesia, Jakarta, Indonesia; 3Department of Veterinary Clinic Reproduction and Pathology, Faculty of Veterinary Medicine, Agriculture Institute of Bogor,…

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Transplanting human induced pluripotent stem cell derived keratinocytes speeds deep second-degree burn wound healing

Human induced pluripotent stem cells successfully differentiated into keratinocyte. Retinoic acid (RA) and BMP-4 were used to induce differentiation of hiPSCs into keratinocyte. The images as immunofluorescence staining of keratinocyte marker proteins K14, involucrin and loricrin at 14 d of induced differentiation, scale bar: 25 µm. Credit: World Journal of…

Continue Reading Transplanting human induced pluripotent stem cell derived keratinocytes speeds deep second-degree burn wound healing

Transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing

BACKGROUND Current evidence shows that human induced pluripotent stem cells (hiPSCs) can effectively differentiate into keratinocytes (KCs), but its effect on skin burn healing has not been reported. AIM To observe the effects of hiPSCs-derived KCs transplantation on skin burn healing in mice and to preliminarily reveal the underlying mechanisms….

Continue Reading Transplantation of human induced pluripotent stem cell derived keratinocytes accelerates deep second-degree burn wound healing

Evolutionary histories of breast cancer and related clones

Data reporting No statistical methods were used to determine the sample size. The experiments were not randomized. Pathologists were blinded to the genetic alterations in each sample during histopathological evaluation. Participants and materials We enroled 207 female patients with breast cancer who underwent surgery at the Kyoto University Hospital and…

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How to choose BWA-MEM2 settings

How to choose BWA-MEM2 settings 0 I am trying to run a BWA-MEM2 script to align DNA-seq data. But I am following this guide. How did they come up with the -t, -T, and -R information? On the manual, -t is threads, -T says “Don’t output alignment with score lower…

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Molecular features driving condensate formation and gene expression by the BRD4-NUT fusion oncoprotein are overlapping but distinct

Cell culture HCC2429 cells were a kind gift from the Hamon Center for Therapeutic Oncology Research at UT Southwestern Medical Center. The cells were grown in RPMI 1640 medium (Thermo Fisher Scientific, #11875119) with addition of 10% heat-inactivated Fetal Bovine Serum (Thermo Fisher Scientific, #10-438-026) and 1% penicillin—streptomycin (Thermo Fisher…

Continue Reading Molecular features driving condensate formation and gene expression by the BRD4-NUT fusion oncoprotein are overlapping but distinct

CPA-Cas12a-based lateral flow strip for portable assay of Methicillin-resistant Staphylococcus aureus in clinical sample | Journal of Nanobiotechnology

Principle of CPA-Cas 12a lateral flow assay strategy CPA and CPA-Cas 12a sensing strategies were proposed for detection of nuc and mecA genes in MRSA respectively. According to the sequence of the target gene, we designed two PAM sites and four pairs of primer according to the principles of double-crossing…

Continue Reading CPA-Cas12a-based lateral flow strip for portable assay of Methicillin-resistant Staphylococcus aureus in clinical sample | Journal of Nanobiotechnology

Antiviral HIV-1 SERINC restriction factors disrupt virus membrane asymmetry

Constructs and expression of human SERINC proteins SERINC3 The gene that encodes human SERINC3 (Genscript-OHu02717D) was inserted upstream of a thrombin protease cleavable linker (LVPRGS) and Strep II epitope (WSHPQFEK) in a modified pFastBac vector by In-Fusion cloning (Clontech). Mutagenesis using the QuikChange Site-Directed Mutagenesis kit (Agilent) was performed to…

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Age-related self-DNA accumulation may accelerate arthritis in rats and in human rheumatoid arthritis

Statement of ethics and regulations Our clinical data collection was approved by the Research Ethics Committee of the Affiliated Hospital of Southwest Medical University (KY 2021010). The animal study was conducted according to the guidelines of the Institutional Animal Care and Use Committee of Macau University of Science and Technology…

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Organization of the human intestine at single-cell resolution

Tissue collection and processing This study complies with all relevant ethical regulations and was approved by the Washington University Institutional Review Board and the Stanford University Institutional Review Board. Human bowel tissues were procured from deceased organ donors. Written informed consent was obtained from the next-of-kin for all donor participants….

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The effective treatment of malignant melanoma.

Plain-Language Summary Malignant melanoma (MM) is the most lethal skin cancer. Recent studies have found that the invasion and metastasis of MM are related not only to MM gene mutation but also to the interaction between MM cells and the immune system and microenvironment. The application of RNAi-based therapeutics to…

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PP computation – User discussions

GROMACS version: 2021GROMACS modification: No Hi, I am trying to compare the performance of GROMACS in two different high performance computers for which I am obtaining very big differences in computational times for different number of nodes (I am not using GPUs yet): around 15 times slower in every case.For…

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Acquisition, co-option, and duplication of the rtx toxin system and the emergence of virulence in Kingella

A common ancestor of pathogenic Kingella species acquired the RTX toxin Consistent with existing literature, when the five Kingella species were assessed for β-hemolysis on BHI plates supplemented with 10% sheep blood, only K. kingae and K. negevensis were hemolytic (Fig. 1A), suggesting that the two pathogenic Kingella species produce the…

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Cationic platinum-based drugs as STING agonists for enhanced chemoimmunotherapy polymers

*For medical professionals to read and reference only STING agonists have made great progress in the diagnosis and treatment of tumor immunity . However, its inherent instability and low bioavailability limit its wide application. Based on this, Yu Yingjie’s team at Beijing University of Chemical Technology introduced a reduction-sensitive polymer…

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Amphioxus adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U and A-to-I deamination of DNA

Animals and cells Adult amphioxus B. japonicum collected from the sandy bottom of the sea near Qingdao, China, were cultured in aerated seawater at room temperature and fed with single-celled algae once a day. Zebrafish (D. rerio) aged 3 months and adult Japanese scallops (M. yessoensis) were purchased from Nanshan…

Continue Reading Amphioxus adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U and A-to-I deamination of DNA

Differentiation of human Dopamine Neurons (DaNs) from induced pluripotent stem cells (iPSCs)

Day 10+1: Splitting Day 10 NPCs Some lines may survive 1:2, but it is advisable to split cells 2:3, as there will be quite some cell death and the cells need to be fairly confluent to maintain viability and cell identity. If split too harshly, they may differentiate into other…

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Cancers | Free Full-Text | A Novel Bispecific Antibody for EpCAM-Directed Inhibition of the CD73/Adenosine Immune Checkpoint in Ovarian Cancer

1. Introduction Typically, tumors in patients with ovarian cancer (OC) are often diagnosed at an advanced stage [1]. Currently, cytoreductive therapy with platinum-based chemotherapy is the mainstay of disease management, to which the majority of patients with OC initially respond. Unfortunately, 70% of these patients eventually develop refractory recurrences [2]….

Continue Reading Cancers | Free Full-Text | A Novel Bispecific Antibody for EpCAM-Directed Inhibition of the CD73/Adenosine Immune Checkpoint in Ovarian Cancer

Uncovering a hidden diversity: optimized protocols for the extraction of dsDNA bacteriophages from soil | Microbiome | Full Text

Soil free Agricultural soil samples were collected out a long-term soil experimental range (ZOFE, Zurich Biological Fertilization Experiment), found into an pastoral area surrounding Zurich (Agroscope, Reckenholz, Switzerland). Soil consisted of 56% sand, 28% silt and 14% clay (in mass %: 0.6 soil organic charcoal, 1.1 soil humus, pH ~ 5.7)….

Continue Reading Uncovering a hidden diversity: optimized protocols for the extraction of dsDNA bacteriophages from soil | Microbiome | Full Text

In vitro and in vivo characterization of SARS-CoV-2 resistance to ensitrelvir

Ethics All animal experiments were conducted in accordance with the University of Tokyo’s Regulations for Animal Care and Use, which were approved by the Animal Experiment Committee of the Institute of Medical Science, the University of Tokyo. The committee acknowledged and accepted both the legal and ethical responsibility for the…

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HIV-1 promotes ubiquitination of the amyloidogenic C-terminal fragment of APP to support viral replication

Ethics Statement LifeSource Buffy Coat blood obtained from New York blood center was used for isolation of peripheral blood mononuclear cells using Ficoll-Plaque (GE). Ethical approval for the study was obtained from the Institutional Review Board of Northwestern University and all donors provided their written, informed consent. Cell cultures HEK293T…

Continue Reading HIV-1 promotes ubiquitination of the amyloidogenic C-terminal fragment of APP to support viral replication

Isolation, Digestion, Filtration and Cell Plating of Murine Adipose Tissue

Begin by placing a seven to eight week old euthanized male mouse on a clean bench surface. Cut the abdominal skin of the mouse with a pair of scissors towards the lower abdomen. Excise the adipose tissue from the inner thighs. Place the excised tissue into tube C on ice…

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Frozen Liver Tissue Sectioning and BODIPY Staining of Lipid Droplets

Transcript Turn on the cryostat and cut 12-micron-thick sections of the frozen liver tissue collected from a rat. Place the sections onto the labeled microscope slides at room temperature. For BODIPY staining, wash the tissue sections thrice with PBS for five minutes each. After the last wash, circle the sections…

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Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric patients undergoing parental liver transplantation | BMC Gastroenterology

Study design and subjects This prospective study was conducted at Renji Hospital, School of Medicine, Shanghai Jiaotong University from July 1, 2019, to October 10, 2020. The study protocol was approved by the Renji Hospital Ethics Committee and adhered to the principles of the Declaration of Helsinki. Written informed consent…

Continue Reading Neutrophil extracellular traps predict postoperative pulmonary complications in paediatric patients undergoing parental liver transplantation | BMC Gastroenterology

Circular RNA RSU1 promotes retinal vascular dysfunction by regulating miR-345-3p/TAZ

Patients and samples A total of 103 diabetes mellitus (type 2) patients were recruited according to the criteria from the American Diabetes Association30. Plasma samples were collected from blood of all the patients, and the patients were classified as diabetes mellitus patients without DR (n = 53) and diabetes mellitus patients with…

Continue Reading Circular RNA RSU1 promotes retinal vascular dysfunction by regulating miR-345-3p/TAZ

nf-core/configs

abims The ABiMS cluster profile slurm adcra CRA HPC profile slurm alice Profile for use on Academic Leiden Interdisciplinary Cluster Environment (ALICE). slurm aws_tower AWS Batch with Tower Profile awsbatch awsbatch AWSBATCH Cloud Profile awsbatch azurebatch Azure BATCH Cloud Profile azurebatch azurebatchdev Azure BATCH Dev Cloud Profile azurebatch bi Boehringer…

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ResR/McdR-regulated protein translation machinery contributes to drug resilience in Mycobacterium tuberculosis

Bacterial strains and culture conditions Escherichia coli strain DH5α (Thermo Fisher) was used for the propagation of plasmids, whereas E. coli BL21 DE3 (Novagen) was used for the expression and purification of ResR/McdR protein. Mtb Erdman was obtained from Dr. Ramandeep Singh at THSTI, India, and Mtb H37Rv mc2 790242…

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An RNA modification enzyme directly senses reactive oxygen species for translational regulation in Enterococcus faecalis

Bacteria strains, plasmids, and growth conditions Enterococcus faecalis strains OG1RF and V583 are grown in tryptic soy broth (TSB) or plated on tryptic soy agar under aerobic conditions at 37 °C. All mutant strains are derivatives of OG1RF. The rlmN knockout in OG1RF (ΔrlmN) was generated by an in-frame deletion of…

Continue Reading An RNA modification enzyme directly senses reactive oxygen species for translational regulation in Enterococcus faecalis

A Game-Changer for Air Travel

Source: pbs.twimg.com As the Noida Airport will likely start operations from 2024, the construction is in full swing. From having a world-class passenger processing system to having approximately 6-8 runways, this new airport will include state-of-the-art facilities.  The Uttar Pradesh government has recently released a notification in support of the…

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Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease | Molecular Neurodegeneration

Aim, design and setting of the study We aim to address our research question – how does microbial diversity affect PD pathology with ageing at the molecular level and can early intervention in the microbiome modulate the disease course? Herein, we have studied longitudinally the gut microbiome dynamics with ageing and report that with ageing,…

Continue Reading Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease | Molecular Neurodegeneration

A simple and available measurement of onco-sEV dsDNA to protein ratio as a potential tumor marker | BMC Cancer

Research object In this study, 34 tumor patients treated in Nanjing Drum Tower Hospital from May 2021 to September 2021 were selected. The average age of tumor group was 59 years old, with 25 male and 9 female. There were 13 cancer types in cancer group, including gastric cancer, lung…

Continue Reading A simple and available measurement of onco-sEV dsDNA to protein ratio as a potential tumor marker | BMC Cancer

Mesenchymal stromal cells facilitate resolution of pulmonary fibrosis by miR-29c and miR-129 intercellular transfer

Cell culture hTERT immortalized adipose-derived mesenchymal stem cells (hTERT-MSC, ASC52telo (ATCC® SCRC-4000™)), hTERT-MSCs with Crispr–Cas9 miR-29с knockdown and hTERT-MSCs with Crispr–Cas9 nonsense knockdown (biobank of the Institute for Regenerative Medicine, Lomonosov MSU (human.depo.msu.ru)) were cultured in Advance Stem Cell Basal Medium with Advance Stem Cell Growth Supplement (HyClone). All experiments…

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University of Oklahoma Associate Director Jobs July, 2023 (Hiring Now!)

University of Oklahoma Associate Director Jobs July, 2023 (Hiring Now!) – Zippia – 125 Jobs Course Developer Associate Director Job At University of Oklahoma ***This position will be located in Norman with a flexible and/or remote option possible for experienced candidates. Special Instructions: If you are selected as a final…

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Fluorescence lifetime FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators

Molecular biology A two-color intramolecular human SERCA2a (2CS) biosensor, based on human cardiac SERCA2a fused to green fluorescent protein (eGFP) and red fluorescent protein (tagRFP) was developed to detect structural changes that are related to the functional changes of SERCA20,22,25. Briefly, tagRFP was genetically fused to the N-terminus of SERCA…

Continue Reading Fluorescence lifetime FRET assay for live-cell high-throughput screening of the cardiac SERCA pump yields multiple classes of small-molecule allosteric modulators

CRISPR prime editing for unconstrained correction of oncogenic KRAS variants

Design of universal pegRNAs for KRAS gene correction We first investigated the mutation frequency of three RAS family genes, HRAS, NRAS, and KRAS, which are involved in tumorigenesis. According to the COSMIC (Catalog Of Somatic Mutations In Cancer) database (cancer.sanger.ac.uk/)22, KRAS mutations account for the majority (81.4%) of RAS mutations,…

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A human proteogenomic-cellular framework identifies KIF5A as a modulator of astrocyte process integrity with relevance to ALS

Human interactome and network-based expansion For the human interactome, we selected STRING v11.0 (combined score > 0.75), BioGRID (v 3.5.172) and IntAct (May 2019) databases derived from high-throughput studies (reporting at least 1,000 interactions). All nodes were mapped to Ensembl gene (ENSG) identifiers, edges were considered as non-directed connections and…

Continue Reading A human proteogenomic-cellular framework identifies KIF5A as a modulator of astrocyte process integrity with relevance to ALS

CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering process

General Lab Operation The assays were performed by experienced individuals throughout the course of the study. The study was performed using established laboratory protocols covering the processing, freezing, storage and thawing of cells as well as the staining procedure, data acquisition and gating strategy. Additional data beyond the data found…

Continue Reading CAR T cells as micropharmacies against solid cancers: Combining effector T-cell mediated cell death with vascular targeting in a one-step engineering process

T cell infiltration into the brain triggers pulmonary dysfunction in murine Cryptococcus-associated IRIS

Ethics statement C57BL6, Rag1−/− (002216), Tcra−/− (002116), CCR5−/− (005427), Slc17a7-IRES2-Cre-D knock-in mice (Vglut1-cre, 023527), Cd4-cre (022071), and floxed EYFP (7903) mice purchased from The Jackson Laboratory were used for the current work. Mice were group-housed with two to five in an individual cage and kept under specific pathogen-free conditions with…

Continue Reading T cell infiltration into the brain triggers pulmonary dysfunction in murine Cryptococcus-associated IRIS

Genomic screening of 16 UK native bat species through conservationist networks uncovers coronaviruses with zoonotic potential

Sample collection Sampling kits were sent out to various bat rehabilitators in the UK, as described previously56, for the collection of faeces from bats. These faecal samples (0.02–1 g) were immediately stored in 5 ml of RNAlater solution to prevent degradation of RNA. The geographical locations and collection dates for all samples…

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Diagnosis in Ectopic Pregnancy | IJGM

Introduction As the leading cause of maternal mortality in early pregnancy, ectopic pregnancy (EP) is responsible for 4–10% of all pregnancy-related deaths,1 but drugs can only be used to treat early EP. However, significant differences in the effects on fertility between surgical treatment and medical treatment have been reported in…

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where is mitochondrial dna found

Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. sFluorescence images of Calcein AM/PI staining in the CsA- or DMSO-treated NPCs. NPC pyroptosis mechanism via mtDNA mediated TLR9-NF-B-NLRP3 axis activation is unclear. In addition, we found that TLR9 recognized and bound mtDNA, which is critical for NF-B and the NLRP3 inflammasome…

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A novel CRISPR/Cas9-based iduronate-2-sulfatase (IDS) knockout human neuronal cell line reveals earliest pathological changes

Cell culture and differentiation Lund Human Mesencephalic (LUHMES) neuronal cells were purchased from ATCC (CRL 2927) and cultured according to previous reports15 and manufacturer’s instructions. Briefly, all plastic culture plates were pre-coated with 50 μg/ml poly-L-ornithine (Merck, Italy) overnight, washed twice with sterilized water at the end of incubation and then…

Continue Reading A novel CRISPR/Cas9-based iduronate-2-sulfatase (IDS) knockout human neuronal cell line reveals earliest pathological changes

(447e) Nanoparticle Delivery of Small Molecule Inhibitors to Treat Sting-Associated Inflammatory Diseases

Problem: The cyclic GMP-AMP synthase (cGAS)/Stimulator of Interferon Genes (STING) pathway is implicated in the development and progression of a myriad of inflammatory diseases including nonalcoholic steatohepatitis, colitis, sepsis, age-related macular degeneration, and cellular senescence. Thus, STING pathway inhibitors (in isolation or in combination with other therapies) could have wide…

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Agarose Fluid Gels Formed by Shear Processing During Gelation for Suspended 3D Bioprinting

Shear processing during hydrogel formation results in the production of microgel suspensions that shear-thin but rapidly restructure following the removal of shear forces. Such materials have been used as a supporting matrix for bioprinting complex, cell-laden structures. Here, methods used to manufacture the supporting bed and compatible bioinks are described….

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The effect of inhibition of receptor tyrosine kinase AXL on DNA damage response in ovarian cancer

Cell lines and culture conditions All media and supplements were purchased from Gibco unless otherwise stated. OC cell line SKOV3, OVCA429, CH1 and NSCLC cell line H1299 and A549 were maintained using Dulbecco’s Modified Eagle Medium (DMEM) media supplemented with 10% (v/v) foetal bovine serum (FBS). OC cell lines HeyA8,…

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Shane Campbell-Staton is showing the world how human activity is shaping evolution right now

One night back in 2016, Shane Campbell-Staton couldn’t sleep. Doing what any person who feels inexplicably restless at 3 a.m. might do, biologist Campbell-Staton embarked down a YouTube rabbit hole. A few videos deep, he came across a clip about the tuskless elephants who live in Gorongosa National Park in…

Continue Reading Shane Campbell-Staton is showing the world how human activity is shaping evolution right now

Single-cell quantification of ribosome occupancy in early mouse development

PDMS chip fabrication Moulds were 3D-printed by Proto Labs with WaterShed XC 11122 at high resolution (Supplementary Fig. 1). Reusable moulds were assembled by taping 3D-printed molds to glass slides (5″ × 4″; Ted Pella). Sylgard 184 PDMS monomer and curing agent (4019862, Ellsworth Adhesives) were mixed at a 10:1…

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Modelling ligand depletion for simultaneous affinity and binding site quantification on cells and tissue

Cell culture All cells were maintained under standard cell culture conditions (37 °C, 5% CO2, saturated humidity). CHO cells stably transfected with human GIPR (gastric inhibitory polypeptide receptor) were obtained from InSCREENeX GmbH (Germany) and cultured in Dulbecco`s Modified Eagle`s Medium/Nutrient Mixture F-12 Ham (Cat.No. D6421, Sigma) supplemented with 5% fetal bovine…

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Adipogenic and SWAT cells separate from a common progenitor in human brown and white adipose depots

Human samples Human adipogenic progenitor cells were isolated from the stromal vascular fraction of adipose tissue samples on the day they were obtained (surgery or biopsy) from four regions: (1) visceral adipose tissue (obtained during gallbladder surgery); (2) perirenal adipose tissue (obtained during nephrectomy surgery); (3) abdominal subcutaneous adipose tissue…

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Nitrate and a nitrate-reducing Rothia aeria strain as potential prebiotic or synbiotic treatments for periodontitis

Bacterial strains and growth conditions Rothia aeria CECT9999 (Ra9, isolate D1P7 described in24), was used as a nitrate-reducing probiotic candidate. Prior to the experiment, Brain Heart Infusion (BHI) (Biolife, Deerfield, Illinois, USA) agar plates were inoculated with the strain, incubated for 48 h at 37 °C, and stored at 4 °C for up…

Continue Reading Nitrate and a nitrate-reducing Rothia aeria strain as potential prebiotic or synbiotic treatments for periodontitis

Prioritization and functional validation of target genes from single-cell transcriptomics studies

List of congruent tip TEC markers The list of congruent tip TEC markers was extracted from a publicly available lung tumor study8. In brief, to identify congruent tip cell markers across freshly isolated human and murine TECs, as well as cultured human TECs, all genes that were most highly expressed…

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Development of Specialized Microelectrode Arrays with Local Electroporation Functionality

Fabrication and Encapsulation of MEAs Borosilicate glass wafers with 100 mm (4ʺ) diameter were used as substrates for the MEA structures. For a lift-off process (Fig. 2a-1 and a-2), AZ5215E photoresist (MicroChemicals GmbH, Ulm, Germany) was spin-coated and patterned by an image reversal UV photolithography step. Then, 10 nm titanium was deposited…

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Non disseminative nano-strategy against in vivo Staphylococcus aureus biofilms

Materials The lipid cetyl palmitate was a kind gift from Gattefossé (Gattefossé SAS, Saint-Priest, France). DSPE-PEG2000-NH2 (1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[amino(polyethylene glycol)-2000] (ammonium salt)) was purchased from Avanti Polar Lipids Inc. (Alabaster, Alabama, USA). D-Phenylalanine, D-Proline, D-Tyrosine, triethylamine (TEA), dicyclohexylcarbodiimide (DCC), N-hydroxysuccinimide (NHS), sodium hydroxide (NaOH), sodium chloride, moxifloxacin hydrochloride (MOX), farnesol 95%, Tween®80,…

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Iterative normalization for large files using BBnorm

Iterative normalization for large files using BBnorm 1 hi all, I’m a big fan of the BBTools suite, but I’m checking that I’m not doing anything silly here. I have several terabytes of decontaminated, QC’d, error corrected reads that I’d like to normalize prior to cross assembly on the agglomerated…

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JCM | Free Full-Text | Reduction of EpCAM-Positive Cells from a Cell Salvage Product Is Achieved by Leucocyte Depletion Filters Alone

1. Introduction Allogeneic blood transfusion is potentially life-saving for bleeding patients, but it is not totally risk-free [1]. Intraoperative cell salvage, also known as autotransfusion, is a strategy designed to reduce the need for allogeneic transfusions. This procedure involves the collection of patient blood from the surgical field, which is…

Continue Reading JCM | Free Full-Text | Reduction of EpCAM-Positive Cells from a Cell Salvage Product Is Achieved by Leucocyte Depletion Filters Alone

Research Computing and Data announces updates, Virtual Town Hall on June 20

June 14, 2023June 14, 2023 Research Computing and Data Virtual Town HallTuesday, June 20, 2023 from 2-3 p.m.Register for the Town Hall To continue sharing information and offering updates, CCIT’s Research Computing and Data (RCD) will host an interactive Town Hall on June 20. During this meeting, members of…

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Antibiotics | Free Full-Text | Whole Genome Sequencing and Phenotypic Analysis of Antibiotic Resistance in Filifactor alocis Isolates

1. Introduction Filifactor alocis is an obligate anaerobic, non-sporeforming, Gram-positive rod [1,2]. This microorganism was first described in 1985 after being isolated from patients with periodontitis or gingivitis [3]. However, it was not until the early to mid-2000s, with the improvement of molecular identification techniques, when F. alocis was first…

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[slurm-users] spreading jobs out across the cluster

I’m currently testing a new slurm setup before converting an existing pbs/torque grid over.  Right now I’ve got 8 nodes in one partition, 48 cores on each.  There’s a second partition of older systems configured as 4 core nodes so the users can run some serial jobs. During some testing…

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BMPER is a marker of adipose progenitors and adipocytes and a positive modulator of adipogenesis

Human subject characteristics Visceral (greater omental) adipose tissue samples were obtained in consented patients for our University of Utah Institutional Review Board (IRB)-approved obesity biorepository at the time of laparoscopic bariatric surgery (obesity group) or laparoscopic abdominal surgery for non-cancerous, non-infected general surgery indications (lean group). Patients were fasted for…

Continue Reading BMPER is a marker of adipose progenitors and adipocytes and a positive modulator of adipogenesis

Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer | Journal of Translational Medicine

Cells and plasmids HEK 293 T and LLC cells (ATCC) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermo Fisher Scientific) containing 10% FBS (Gibco, USA) and 1% penicillin–streptomycin (Invitrogen) at 37 °C in a 5% CO2 environment. Stbl3 and DH5α competent cells were used for plasmid transformation. The pCDH-CMV-MCS-EF1-GFP-Puro vector (cat….

Continue Reading Inhalable CAR-T cell-derived exosomes as paclitaxel carriers for treating lung cancer | Journal of Translational Medicine

Mapping interindividual dynamics of innate immune response at single-cell resolution

Ethical compliance This project was approved by the Wellcome Sanger Institute Animal Welfare and Ethical Review Body and complied with all relevant ethical regulations regarding animal research and human studies. Human cells were obtained from HipSci24, where they were collected from volunteers recruited from the National Institute for Health and…

Continue Reading Mapping interindividual dynamics of innate immune response at single-cell resolution

16S rRNA gene sequencing of stool samples collected from patients with latent tuberculosis infection before, during, and two months after treatment with 3HP or 4R | BMC Research Notes

LTBI diagnosis and treatment FDOH clients who tested positive on a tuberculin skin test (TST) or interferon-gamma release assay (IGRA) blood test were diagnosed with LTBI following a chest x-ray and symptoms screening to rule out TB disease [6]. Healthy controls were LTBI negative by self-report of a negative TST…

Continue Reading 16S rRNA gene sequencing of stool samples collected from patients with latent tuberculosis infection before, during, and two months after treatment with 3HP or 4R | BMC Research Notes

SENP2 restrains the generation of pathogenic Th17 cells in mouse models of colitis

Mice Senp2f/f mice were described as previously16. T cell-specific Senp2 knockout mice (CKO, Senp2f/fLckCre+/+) and control mice (WT, LckCre+/+) in C57BL/6 genetic background were raised and kept in the Academia Sinica SPF animal facility, according to the rule of the Animal Protection Act of Taiwan. Lck-Cre mice, Rag2−/− mice and CD45.1…

Continue Reading SENP2 restrains the generation of pathogenic Th17 cells in mouse models of colitis

Efflux pump gene amplifications bypass necessity of multiple target mutations for resistance against dual-targeting antibiotic

Strains and growth conditions All strains and plasmids used in this work are described in Supplementary Table 1. The clinical isolates were obtained from the Cystic Fibrosis Foundation Isolate Core at Seattle Children’s Hospital and were originally isolated from two different cystic fibrosis patients. Distribution of these isolates is covered by…

Continue Reading Efflux pump gene amplifications bypass necessity of multiple target mutations for resistance against dual-targeting antibiotic

Exogenously delivered iPSCs disrupt the natural repair response of endogenous MPCs after bone injury

Ethics statement All animal studies were performed in accordance with the recommendations in the Canadian Council on Animal Care Guidelines. The reporting of this data in the manuscript follows the recommendations in the ARRIVE guidelines. The University of Calgary Health Sciences Animal Care Committee approved all animal protocols and surgical…

Continue Reading Exogenously delivered iPSCs disrupt the natural repair response of endogenous MPCs after bone injury

Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects

Amino acid sequence comparisons between GAS and GBS Cas9 identify orthologous active sites Relationships between the amino acid sequence of GBS Cas9 endonuclease and its molecular functions can be deduced from detailed studies of its GAS ortholog, SpyCas9 (Supplementary Fig. 1). As a first step in locating DNA complementary to a…

Continue Reading Group B Streptococcus Cas9 variants provide insight into programmable gene repression and CRISPR-Cas transcriptional effects

Integrated multi-omics for rapid rare disease diagnosis on a national scale

Ethics The Australian Genomics Acute Care study has Human Research Ethics Committee approval (HREC/16/MH/251). Parents provided informed consent for participation in the study, following genetic counseling. Study design and participants The Acute Care Genomics program is a national multi-site study delivering ultra-rapid genomic testing to critically ill pediatric patients with…

Continue Reading Integrated multi-omics for rapid rare disease diagnosis on a national scale

Vancomycin intermediate-resistant Staphylococcus haemolyticu | IDR

Wanyang Dong,1,* Qi Peng,1,* Xiaohua Tang,1,2,* Tian Zhong,1 Shunan Lin,1 Ziling Zhi,1 Jingyi Ye,1 Bixia Yang,1 Ning Sun,1,3 Wenchang Yuan1 1Guangzhou Key Laboratory for Clinical Rapid Diagnosis and Early Warning of Infectious Diseases, KingMed School of Laboratory Medicine, Guangzhou Medical University, Guangzhou, 510180, People’s Republic of China; 2Third Affiliated Hospital…

Continue Reading Vancomycin intermediate-resistant Staphylococcus haemolyticu | IDR

Class B1 GPCR activation by an intracellular agonist

Expression and purification of human PTH1R The plasmid encoding human PTH1R (GenBank identifier: U17418.1; residues 27–491) was constructed and purified as previously reported17. The construct was expressed in HEK293 GnTI (N-acetylglucosaminyltransferase I-negative) cells (American Type Culture Collection, CRL-3022) using the BacMam system (Thermo Fisher Scientific), and the cells were grown…

Continue Reading Class B1 GPCR activation by an intracellular agonist

Preferential rabbit antibody responses to C-termini of NOTCH3 peptide immunogens

NOTCH3 anti-peptide antibodies NOTCH3 is a transmembrane receptor (Fig. 1A) that plays multiple roles in development, homeostasis, and pathology. Mutations in NOTCH3 are responsible for the most common cause of inherited stroke and vascular dementia, cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL)8,9. Figure 1 Generation and characterization of…

Continue Reading Preferential rabbit antibody responses to C-termini of NOTCH3 peptide immunogens

Characterization of nuclear DNA diversity in an individual Leymus chinensis

Introduction The definition of an individual organism, traditionally defined by the invariable presence of a physiological unit and genetic homogeneity, is challenged by situations such as intraorganismal genetic heterogeneity (IGH), which refers to the presence of more than one genotype in a single organism (Schweinsberg et al., 2015). Cells within a…

Continue Reading Characterization of nuclear DNA diversity in an individual Leymus chinensis

MTAP-ANRIL gene fusion promotes melanoma epithelial-mesenchymal transition-like process by activating the JNK and p38 signaling pathways

Cell lines Human melanoma cell lines (A375 and A875) were kindly donated by the Department of Dermatology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology. Cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% FBS, 100 μg/ml penicillin and 100 μg/ml streptomycin at 37 °C in a…

Continue Reading MTAP-ANRIL gene fusion promotes melanoma epithelial-mesenchymal transition-like process by activating the JNK and p38 signaling pathways

Robust IL-2-dependent antitumor immunotherapy requires targeting the high-affinity IL-2R on tumor-specific CD8+ T cells

Introduction Interleukin (IL)-2 was the first Food and Drug Administration approved immunotherapy for use in patients with metastatic melanoma and renal cell carcinoma.1 2 However, the use of recombinant IL-2 (Proleukin/aldesleukin) has been problematic due to low response rates, off-target effects, and severe dose-dependent toxicities. Due to its potent T-cell…

Continue Reading Robust IL-2-dependent antitumor immunotherapy requires targeting the high-affinity IL-2R on tumor-specific CD8+ T cells

Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

We present a protocol for assay for transposase-accessible chromatin with high-throughput sequencing (ATAC-seq) specifically on adipocytes using nucleus sorting with adipose tissues isolated from transgenic reporter mice with nuclear fluorescence labeling. ATAC-seq is a powerful technique to understand gene expression regulatory mechanisms. However, adipose tissue is difficult using this technique…

Continue Reading Adipocyte-Specific ATAC-Seq with Adipose Tissues Using Fluorescence-Activated Nucleus Sorting

De novo protein design by inversion of the AlphaFold structure prediction network

Abstract De novo protein design enhances our understanding of the principles that govern protein folding and interactions, and has the potential to revolutionize biotechnology through the engineering of novel protein functionalities. Despite recent progress in computational design strategies, de novo design of protein structures remains challenging, given the vast size…

Continue Reading De novo protein design by inversion of the AlphaFold structure prediction network

Selection and validation of reference genes for RT-qPCR normalization of porcine alveolar macrophages (PAMs) for PRRSV studies

Sample collection and cDNA synthesis In this study, we used porcine alveolar macrophages (PAMs) that were isolated from 16 different animals between the years 2011 and 2022, and subsequently stored. The PAMs were isolated by bronchoalveolar lavage (BAL) from lungs of euthanized 3-week-old healthy conventional piglets from a PRRSV negative…

Continue Reading Selection and validation of reference genes for RT-qPCR normalization of porcine alveolar macrophages (PAMs) for PRRSV studies

Differential Binding of NLRP3 to non-oxidized and Ox-mtDNA mediates NLRP3 Inflammasome Activation

NLRP3 expression and purification Both wild-type NLRP3 and CAPS mutants were cloned in the mammalian expression vector pcDNA3.1HisB. Plasmid was purified using Qiagen Giga Prep up to 2000 ng/μl and subsequently transfected into Expi293 with Expifectamine per manufacturer instructions. Expression was allowed to proceed for 3 days before harvesting. Cells were…

Continue Reading Differential Binding of NLRP3 to non-oxidized and Ox-mtDNA mediates NLRP3 Inflammasome Activation

ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells | Biology Direct

Cell lines, culture conditions, heat shock treatment and transfections HeLa, HEK293 and 2D12 (a NOA36-knockout HEK293-cell line generated in this work) cells were cultured in Dulbecco’s modified Eagle’s medium supplemented with 10% fetal bovine serum and 100 U/mL penicillin and 100 µg/mL streptomycin at 37 °C in a CO2 incubator. For heat…

Continue Reading ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells | Biology Direct

Chemical Equations Balanced on 05/29/23

Balance equation of any chemical reaction online for freeNO2 + H2O = HNO3 + NO =>3 NO2 + H2O = 2 HNO3 + NO (NH4)2CO3 = NH3 + H2O + CO2 =>(NH4)2CO3 = 2 NH3 + H2O + CO2 NaOH + H3PO4 = Na3PO4 + H2O =>3 NaOH + H3PO4…

Continue Reading Chemical Equations Balanced on 05/29/23

Human bone marrow-derived mesenchymal stem overexpressing microRNA-124-3p inhibit DLBCL progression by downregulating the NFATc1/cMYC pathway | Stem Cell Research & Therapy

Microarray-based expression analysis and GEO database analysis The DLBCL-related miRNA expression profiles of GSE29493 and GSE40239 were retrieved from the Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo/). MiR-124’s pan cancer analysis is conducted through online websites (www.picb.ac.cn/dbDEMC/). Bioinformatics predicted NFATc1 as the target of miR-124-3p The miRbase (www.mirbase.org/), miRDB (mirdb.org), and…

Continue Reading Human bone marrow-derived mesenchymal stem overexpressing microRNA-124-3p inhibit DLBCL progression by downregulating the NFATc1/cMYC pathway | Stem Cell Research & Therapy

Clinical characteristics and host immunity responses of SARS-CoV-2 Omicron variant BA.2 with deletion of ORF7a, ORF7b and ORF8 | Virology Journal

Study cohorts This study was conducted in compliance with the Declaration of Helsinki and was approved by the Maoming People’s Hospital (Approval No. 2021-hs-43) and Dongguan Ninth People’s Hospital (Approval No. 2022-8). COVID-19 was confirmed based on positive results of reverse transcription-polymerase chain reaction (RT-PCR) of nasopharyngeal samples. Whole blood…

Continue Reading Clinical characteristics and host immunity responses of SARS-CoV-2 Omicron variant BA.2 with deletion of ORF7a, ORF7b and ORF8 | Virology Journal

Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors

Vector construction The Cas9/sgRNA vector (all-in-one) (GenBank accession no. OQ579018) contained sgRNA and Cas9-2A-mCherry expression cassettes. The sgRNA (5′-ATGCAGAACTAGAGTACAGC-3′) targets the phiC31 pseudo-attP intergenic site located on chromosome 3 of CHO-K1 genome (GenBank accession no. APMK01032147.1) (Fig. 1a). To construct the Cas9-mSA/sgRNA vector (GenBank accession no. OQ579019), the monomeric streptavidin (mSA)…

Continue Reading Efficient site-specific integration in CHO-K1 cells using CRISPR/Cas9-modified donors

Human CD25 (IL-2R alpha) Recombinant Protein (34-8702-82)

Product Specific Information Description: The human soluble IL-2 receptor alpha chain (sIL-2Ra) is generated by proteolytic cleavage of the cell-surface bound receptor on activated T cells. The IL-2Ra (also known as CD25) is expressed by early progenitors of the T and B lineage, as well as by activated mature T…

Continue Reading Human CD25 (IL-2R alpha) Recombinant Protein (34-8702-82)

Phylogenomic analysis supports Mycobacterium tuberculosis transmission between humans and elephants

1. Introduction Tuberculosis (TB) is a significant global burden and is widely reported to be a major public health and economic problem, costing the world $617 billion between 2000 and 2015 and projected to cost $1 trillion between 2015 and 2030 (1). It is the second leading cause of death…

Continue Reading Phylogenomic analysis supports Mycobacterium tuberculosis transmission between humans and elephants

Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

This protocol describes the fabrication and characterization of a photoresponsive prodrug-dye nanoassembly. The methodology for drug release from the nanoparticles by light-triggered disassembly, including the light irradiation setup, is explicitly described. The drugs released from the nanoparticles following light irradiation exhibited excellent anti-proliferation effects on human colorectal tumor cells. This…

Continue Reading Facile Preparation and Photoactivation of Prodrug-Dye Nanoassemblies

Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

In this study, a rat model of induction of periodontitis is presented via a combination of retentive ligature and repetitive injections of lipopolysaccharide derived from Porphyromonas gingivalis, over 14 days around the first maxillary molars. The ligation and LPS injection techniques were effective in inducing peridontitis, resulting in alveolar bone…

Continue Reading Induction of Periodontitis via a Combination of Ligature and Lipopolysaccharide Injection in a Rat Model

Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance

This protocol describes the isolation of mouse preadipocytes from subcutaneous fat, their differentiation into mature adipocytes, and the induction of insulin resistance. Insulin action is evaluated by the phosphorylation/activation of members of the insulin signaling pathway through western blot. This method allows direct determination of insulin resistance/sensitivity in primary adipocytes….

Continue Reading Differentiated Mouse Adipocytes in Primary Culture: A Model of Insulin Resistance

anti-Homo sapiens (Human) Phospho-FGFR1/FGFR2 (Y463/466) Antibody raised in Rabbit

货号: CSB-PA008246 规格: 图片: 其他: 产品详情 Uniprot No.: 宿主: Rabbit 反应种属: Human,Mouse,Rat 免疫原: Synthesized peptide derived…

Continue Reading anti-Homo sapiens (Human) Phospho-FGFR1/FGFR2 (Y463/466) Antibody raised in Rabbit

Deletion of endothelial leptin receptors in mice promotes diet-induced obesity

Experimental animals The generation of mice with tamoxifen-inducible, Tie2.Cre-ERT2-mediated deletion of LepR in endothelial cells was described previously12,17. For Cre recombinase activation, mice (6 weeks-of-age) were fed tamoxifen citrate-containing rodent chow (Envigo; TD.130860) for 6 weeks49. Genomic DNA from the brain, lung, small intestine, subcutaneous adipose tissue (SCAT) and visceral adipose tissue…

Continue Reading Deletion of endothelial leptin receptors in mice promotes diet-induced obesity

Cancers | Free Full-Text | mRNA-Lipid Nanoparticle (LNP) Delivery of Humanized EpCAM-CD3 Bispecific Antibody Significantly Blocks Colorectal Cancer Tumor Growth

Author Contributions Conceptualization, L.W., W.B. and V.G.; methodology, Y.H., J.S. (John Sienkiewicz), J.S. (Jinying Sun), H.Z., H.H., S.X., R.B. and L.H.; software, Y.H., J.S. (John Sienkiewicz) and J.S. (Jinying Sun); validation, H.Z. and J.S. (John Sienkiewicz); formal analysis, V.G.; investigation, V.G. and L.W.; resources, L.W.; data curation, Y.H., J.S. (John…

Continue Reading Cancers | Free Full-Text | mRNA-Lipid Nanoparticle (LNP) Delivery of Humanized EpCAM-CD3 Bispecific Antibody Significantly Blocks Colorectal Cancer Tumor Growth

Pinostrobin, a fingerroot compound, regulates miR-181b-5p and induces acute leukemic cell apoptosis

Leukemia cell culture The human acute promyelocytic leukemia cell line (NB4) and the human acute lymphocytic leukemia cell line (MOLT-4) were purchased from Cell Lines Service (Eppelheim, Germany). Leukemia cells were cultured in RPMI-1640 medium supplemented with 10% (v/v) FBS and 1% (v/v) penicillin–streptomycin (Gibco Life Technologies, Waltham, MA, USA)….

Continue Reading Pinostrobin, a fingerroot compound, regulates miR-181b-5p and induces acute leukemic cell apoptosis

Bioinformatics Analyst II – Remote job with Baylor College of Medicine

Summary The Human Genome Sequencing Center (HGSC) provides a unique opportunity to gain high-throughput data production experience in an academic research environment, with opportunities for publication, conference travel and international scientific collaboration. As a member of the NGSI Clinical Informatics team, you will directly contribute to the HGSC’s pioneering work…

Continue Reading Bioinformatics Analyst II – Remote job with Baylor College of Medicine

Activation of the urotensin-II receptor by remdesivir induces cardiomyocyte dysfunction

Study design The overall goal of this study was to explore the molecular mechanisms of remdesivir-related cardiotoxicity in anti-COVID-19 therapy. We first performed the GPCR screening using major anti-COVID-19 drugs as ligands (n = 3 per GPCR). We validated the results of GPCR screening by concentration-response analysis and determined the EC50 and…

Continue Reading Activation of the urotensin-II receptor by remdesivir induces cardiomyocyte dysfunction

Convergent evolution of SARS-CoV-2 Omicron subvariants leading to the emergence of BQ.1.1 variant

Ethics statement All experiments with hamsters were performed in accordance with the Science Council of Japan’s Guidelines for the Proper Conduct of Animal Experiments. The protocols were approved by the Institutional Animal Care and Use Committee of National University Corporation Hokkaido University (approval ID: 20-0123 and 20-0060). All protocols involving…

Continue Reading Convergent evolution of SARS-CoV-2 Omicron subvariants leading to the emergence of BQ.1.1 variant

Interferon-gamma is quintessential for NOS2 and COX2 expression in ER- breast tumors that lead to poor outcome

Cell culture The MDA-MB231 (MB231) human breast cancer cell line was obtained from the American Type Culture Collection (ATCC, Manassas, VA) and grown in RPM1-1640 (Invitrogen) supplemented with 10% fetal bovine serum (FBS; Invitrogen, Waltham, MA) at 37 °C in a humidified atmosphere of 5% CO2 in the air. Cells were…

Continue Reading Interferon-gamma is quintessential for NOS2 and COX2 expression in ER- breast tumors that lead to poor outcome

Chemical Equations Balanced on 05/10/23

Balance equation of any chemical reaction online for freeCa3P2(s) + H2O(l) = Ca(OH)2(aq) + PH3(g) =>Ca3P2(s) + 6 H2O(l) = 3 Ca(OH)2(aq) + 2 PH3(g) Na + O2 = Na2O =>4 Na + O2 = 2 Na2O H2SO4 + NaOH = Na2SO4 + H2O =>H2SO4 + 2 NaOH = Na2SO4…

Continue Reading Chemical Equations Balanced on 05/10/23

Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells

Cells and cell culture KOPN30, BV173, and K562 are BCR/ABL1-positive leukemia cell lines. All leukemia cell lines, as well as Ba/F3 cells, were maintained in RPMI-1640 medium supplemented with 15% fetal bovine serum (FBS) and penicillin–streptomycin (100 U/mL) at 37 °C in an atmosphere containing 5% CO2. KOPN30 cells were obtained…

Continue Reading Targeting Poly(ADP)ribose polymerase in BCR/ABL1-positive cells