Tag: PDBep

Receptor regulation clues may scratch an itch

Whether you’ve been stung by an insect or suffer from allergies, most people have experienced itchy skin, which usually can be relieved with time or simple remedies. However, for certain patients with liver disease, an intense itching sensation, known as cholestatic pruritis — the medical term for itch — often…

Continue Reading Receptor regulation clues may scratch an itch

Direct observation of the conformational states of PIEZO1

No statistical methods were used to predetermine sample size. The experiments were not randomized and investigators were not blinded to allocation during experiments and outcome assessment. Expression constructs The coding sequence of mouse PIEZO1 (E2JF22, UniprotKB entry) was codon-optimized, synthesized and cloned into the pcDNA3.1 plasmid. An amber stop codon…

Continue Reading Direct observation of the conformational states of PIEZO1

CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems

Architecture of cyanobacterial CAST systems Starting from the known CAST components, we searched for conserved genes and genetic elements in their vicinity. These elements included the left and right ends of the transposon, the neighboring tRNA, CRISPR arrays and tracrRNA, the transposase genes (tnsB, tnsC and tniQ), and genes in…

Continue Reading CvkR is a MerR-type transcriptional repressor of class 2 type V-K CRISPR-associated transposase systems