Tag: pLentiCRISPRv2
pLentiCRISPRV2-IFIT3
PVT29967 2ug pLentiCRISPRV2-IFIT3 Description Promoter:U6Replicator:pUCClone strain:Stbl3Culture conditions:37centigrade Host:mammalian cells,LentivirusUse:Gene editingSpecies:HumanGene type:CRISPR,Cas9,sgRNAProkaryotic resistance:AmpEukaryotic resistance:PuroFluorescent protein: Caution:1. This product is FOR RESEARCH USE ONLY!2. The item is lyophilized form, Please take the powder plasmid by centrifugation at 5000rpm/min for 1min. Add 20ul ddH2O in to the tube of plasmid. Read…
Evasion of cGAS and TRIM5 defines pandemic HIV
Cells and reagents HEK293T and U87 cells were maintained in DMEM medium (Gibco) supplemented with 10% fetal bovine serum (FBS, Labtech) and 100 U ml−1 penicillin plus 100 μg ml−1 streptomycin (Pen/Strep; Gibco). THP-1-IFIT1 cells that had been modified to express Gaussia luciferase under the control of the IFIT1 promoter were described previously62. THP-1…
