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Tag: PLS-DA
Lower-limb ulcers in participants with leprosy sequelae
Introduction Leprosy is a disabling infectious disease that predominantly occurs in the skin and peripheral nerves and is transmitted by contact with pathogenic bacteria through the respiratory tract and broken skin. Leprosy is attributed to infection with Mycobacterium leprae (M. leprae)1 and the more recently discovered Mycobacterium lepromatosis.2 In addition,…
Impact of humid climate on rheumatoid arthritis faecal microbiome and metabolites
Animals and husbandry details Six-week-old healthy male SD rats were used in accordance with the Guidelines for the Care and Use of Laboratory Animals of the Institute of Laboratory Animal Resources, Institutional Animal Care and Use Committee of Beijing University of Chinese Medicine. The study is reported in accordance with…
How to analyze heterogenous forms of experimental data (not sequencing based)?
How to analyze heterogenous forms of experimental data (not sequencing based)? 0 Hello all- looking for some general tips/inquiring about feasibility for analyzing heterogenous biological data that may/may not have a sequencing component. I have been tasked with analyzing various forms of data from the same experiment, including: -Imaging data…
Overexpression of human alpha-Synuclein leads to dysregulated microbiome/metabolites with ageing in a rat model of Parkinson disease | Molecular Neurodegeneration
Aim, design and setting of the study We aim to address our research question – how does microbial diversity affect PD pathology with ageing at the molecular level and can early intervention in the microbiome modulate the disease course? Herein, we have studied longitudinally the gut microbiome dynamics with ageing and report that with ageing,…
Influence of grape consumption on the human microbiome
Grape intervention study design The trial was conducted over a period of two months. Forty normal, healthy, free-living human subjects entered the trial in which plasma, urine and fecal material were sequentially collected following two-weeks of restricted diet (Day 15), two-weeks of restricted diet supplemented with the equivalent of three…
The role of FMT in T2D and its underlying mechanisms.
Background The prevalence of type 2 diabetes (T2D) is rapidly increasing worldwide, with an estimated global prevalence of approximately 552 million by 2030.1 Long-term hyperglycemia in T2D patients can lead to vascular endothelial damage, which includes microvascular disease such as diabetic retinopathy and nephropathy, neuropathy, and macrovascular disease including peripheral…
Characterization of rumen microbiome and metabolome from oro-esophageal tubing and rumen cannula in Holstein dairy cows
All experimental procedures were conducted at the University of Illinois at Urbana-Champaign and followed protocols approved by the Institutional Animal Care and Use Committee (IACUC) at the University of Illinois at Urbana-Champaign under protocol number 17172. All ARRIVE, and IACUC guidelines and regulations were followed during the entire duration of…
Whole-body adipose tissue multi-omic analyses in sheep reveal molecular mechanisms underlying local adaptation to extreme environments
RNA-Seq data Across all 250 adipose tissue samples, a total of 1780 Gb of clean reads were retained with an average of 23.7 million reads (14.6–43.5 million reads) per sample after quality control (Fig. 1a, b and Supplementary Data 1, 2). Of the total clean reads, 83.42–97.42% of reads were mapped to the…
Isolation and characterisation of exosomes from Chinese hamster ovary (CHO) cells
Belliveau J, Papoutsakis ET (2022) Extracellular vesicles facilitate large-scale dynamic exchange of proteins and RNA among cultured Chinese hamster ovary and human cells. Biotechnol Bioeng 119(5):1222–1238. doi.org/10.1002/bit.28053 Article CAS Google Scholar Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle…