Tag: recovery

Three families of Asgard archaeal viruses identified in metagenome-assembled genomes

Liu, Y. et al. Expanded diversity of Asgard archaea and their relationships with eukaryotes. Nature 593, 553–557 (2021). CAS  PubMed  Google Scholar  Dombrowski, N., Teske, A. P. & Baker, B. J. Expansive microbial metabolic versatility and biodiversity in dynamic Guaymas Basin hydrothermal sediments. Nat. Commun. 9, 4999 (2018). PubMed  PubMed…

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India’s economy rebounded strongly in 2nd half of year

The Indian economy has rebounded strongly despite three significant Covid-19 waves, the US Treasury said in a report to the Congress on Friday. India’s acute second wave weighed heavily on growth through the middle of 2021, delaying its economic recovery, the Treasury said in a semi-annual report. “However,…

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Binding of the HSF-1 DNA-binding domain to multimeric C. elegans consensus HSEs is guided by cooperative interactions

The heat-shock response is represented by a small set of genes in C. elegans We initially aimed at identifying those HSE-containing promoters that are most strongly upregulated under heat-stress conditions. Given that the HSR is complex in nematodes we used data from several heat-shock studies based on microarray and RNAseq…

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Bispecific antibodies | Scientist Live

Bispecific antibodies: rising stars in antibody therapeutics Monoclonal antibodies (mAbs) are important therapeutic agents for the treatment of many human diseases, such as cancer, autoimmune diseases, cardiovascular diseases, asthma, and viral infections. Unlike monospecific mAbs, bispecific antibodies (bsAbs) are antibodies containing two antigen-binding sites and therefore can simultaneously target two…

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India Is Committed To Ensuring Accessibility & Affordability Of Vaccines For All: Union Minister

New Delhi: Union Minister of State Science & Technology Dr Jitendra Singh said that India is committed to ensuring the accessibility and affordability of vaccines for all. Addressing the Seventh Annual Multi-Stakeholder Forum on Science, Technology and Innovation for the Sustainable Development Goals of the UN, Dr Jitendra Singh said,…

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Plant Breeding and CRISPR Plants Market Trends 2022, Global Industry Share, Size, Global Industry Analysis, Key Market Growth Analysis, Segments, Emerging Technologies, Opportunity and Forecast 2027

The “Plant Breeding and CRISPR Plants Market” research report 2021-2027 offering the complete outline of market size, share and growth opportunities, industry segmentation by product type, applications and geographical representation. The report highlights product development along with technological upgrades that can boost the market growth. The Plant Breeding and CRISPR…

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Comprehensive Evaluation and Application of a Novel Method to Isolate Cell-Free DNA Derived From Bile of Biliary Tract Cancer Patients

Cell-free DNA (cfDNA) exists in various types of bodily fluids, including plasma, urine, bile, and others. Bile cfDNA could serve as a promising liquid biopsy for biliary tract cancer (BTC) patients, as bile directly contacts tumors in the biliary tract system. However, there is no commercial kit or widely acknowledged…

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CTS Xenon Electroporation System | Thermo Fisher Scientific

The CTS Xenon Electroporation System offers reliable delivery of DNA, RNA, proteins, and other molecules into cells, with exceptional cell viability and recovery. This leads to efficient transfection and genome editing, even in hard-to-transfect cells. A choice of two single-use electroporation chambers of different capacities provides flexibility: the SingleShot chamber…

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Mitogenome-wise codon usage pattern from comparative analysis of the first mitogenome of Blepharipa sp. (Muga uzifly) with other Oestroid flies

Outcome of DNA sequencing, assembly, and validation In this study, initially total DNA was isolated from the finely chopped, full-grown pupa of Blepharipa sp. The NanoDrop spectrophotometer (1294 ng/μl) and the Qubit fluorometer (732.8 ng/μl) both found that the concentration of total DNA in the sample at an optimum level for mitochondrial DNA enrichment. The Tape Station profile showed…

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Diffuse Large B-Cell Lymphoma: Causes, Symptoms, Treatment

Diffuse large B-cell lymphoma (DLBCL) is a cancer of the B cells of the lymphatic system. B cells are responsible for producing antibodies, which are highly specific proteins that fight against bacterial and viral infections. When cancer develops in the B cells, it is called a B-cell lymphoma. B-cell lymphoma…

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Engineering and validation of a dual luciferase reporter system for quantitative and systematic assessment of regulatory sequences in Chinese hamster ovary cells

Experimental workflow and vector designs One way to validate the all-in-one reporter system is through transient or stable transfection of mammalian cells. Within this framework, the schematics in Fig. 1 illustrate the overall workflow of this study and feature the characteristics of promoters. To summarize, the study involves the engineering and…

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Critical Assessment of Metagenome Interpretation: the second round of challenges

Evaluating metagenomic software is key for optimizing metagenome interpretation and focus of the Initiative for the Critical Assessment of Metagenome Interpretation (CAMI). The CAMI II challenge engaged the community to assess methods on realistic and complex datasets with long- and short-read sequences, created computationally from around 1,700 new and known…

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Innovative site-specific anti-HER2 antibody-drug conjugate

Introduction Antibody-drug conjugates (ADCs) have been studied for decades as a promising cancer treatment. As compared to conventional chemotherapy, ADCs have a larger therapeutic window because of their ability to target antigen-expressing tumor cells.1 As ADC drug development technology continues to evolve, several ADC drugs are being introduced to the…

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Acute systemic knockdown of Atg7 is lethal and causes pancreatic destruction in shRNA transgenic mice

The notion that macroautophagy/autophagy is a potentially attractive therapeutic target for a variety of diseases, including cancer, largely stems from pre-clinical mouse studies. Most of these examine the effects of irreversible and organ confined autophagy deletion using site specific Cre-loxP recombination of the essential autophagy regulating genes Atg7 or Atg5….

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Major hindgut diversity in horses laid bare in major study of microorganisms

Image by Greg Gwynne from Pixabay Surprising hindgut diversity among the microorganisms that inhabit the hindgut of Thoroughbred horses have been described by researchers, including dozens of new or unnamed bacteria. Scientists used molecular-based analysis of faecal samples from five 12-month-old Thoroughbreds from the same farm and field in Ireland….

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Yandere-male-x-straight-male-reader

Kolkata FF is a game of Satta Matka in which person guess the correct number. Hence, then is rewarded with a … 1 Min Read. Regex number greater than 1000.. It was higher than in 91.8% U.S. cities. The 2019 Syracuse crime rate rose by 5% compared to 2018. The…

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Mitogenomics provides new insights into the phylogenetic relationships and evolutionary history of deep-sea sea stars (Asteroidea)

Mah, C. L. & Blake, D. B. Global diversity and phylogeny of the asteroidea (Echinodermata). PLoS ONE 7, e35644. doi.org/10.1371/journal.pone.0035644 (2012). ADS  CAS  Article  PubMed  PubMed Central  Google Scholar  WoRMS. Asteroidea. Accessed at: www.marinespecies.org/aphia.php?p=taxdetails&id=123080. on 2021-08-02. Blake, D. B. Adaptive zones of the class Asteroidea (Echinodermata). Bull. Mar. Sci. 46,…

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Real-Time Intravital Multiphoton Microscopy to Visualize Focused Ultrasound and Microbubble Treatments to Increase Blood-Brain Barrier Permeability

This protocol describes the surgical and technical procedures that enable real-time in vivo multiphoton fluorescence imaging of the rodent brain during focused ultrasound and microbubble treatments to increase blood-brain barrier permeability. Ultrasound exposure in the presence of microbubbles has emerged as an effective method to transiently and locally increase the…

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Accurate prediction of metagenome-assembled genome completeness by MAGISTA, a random forest model built on alignment-free intra-bin statistics | Environmental Microbiome

Steen AD, Crits-Christoph A, Carini P, DeAngelis KM, Fierer N, Lloyd KG, Cameron TJ. High proportions of bacteria and archaea across most biomes remain uncultured. ISME J. 2019;13:3126–30. PubMed  PubMed Central  Google Scholar  Goh KM, Shahar S, Chan K-G, Chong CS, Amran SI, Sani MH, Zakaria II, Kahar UM. Current…

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Integrative Toolkit to Analyze Cellular Signals: Forces, Motion, Morphology, and Fluorescence

Adherent cells exert forces on their surrounding, and those forces include the forces cells exert on the substrate, shown here in green, and the forces cells exert on their neighbors, shown in red. These forces can be measured in cellular monolayers using a technique called monolayer stress microscopy. Briefly, the…

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Genomic variation from an extinct species is retained in the extant radiation following speciation reversal

Vamosi, J. C., Magallon, S., Mayrose, I., Otto, S. P. & Sauquet, H. Macroevolutionary patterns of flowering plant speciation and extinction. Annu. Rev. Plant Biol. 69, 685–706 (2018). CAS  PubMed  Google Scholar  Rhymer, J. M. & Simberloff, D. Extinction by hybridization and introgression. Annu. Rev. Ecol. Syst. 27, 83–109 (1996)….

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CRISPR-Cas9 Gene Therapy for Duchenne Muscular Dystrophy

Ishino Y, Shinagawa H, Makino K, et al. Nucleotide sequence of the iap gene, responsible for alkaline phosphatase isozyme conversion in Escherichia coli, and identification of the gene product. J Bacteriol. 1987;169:5429–33. CAS  PubMed  PubMed Central  Google Scholar  Jansen R, van Embden JDA, Gaastra W, et al. Identification of genes…

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HyDrop: droplet-based scATAC-seq and scRNA-seq using dissolvable hydrogel beads

Reviewer #1 (Public Review): Droplet-based single-cell method development has stalled in the past years. The field has been overtaken by commercial solutions that optimized performance, but at much higher costs and without any possibility for customization. More recently, combinatorial indexing methods (e.g. SPLIT-seq) have gained popularity, requiring no specialized equipment…

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Photodegradable Hydrogel Interfaces for Bacteria Screening, Selection, and Isolation

The use of photodegradable hydrogels to isolate bacterial cells by utilizing a high-resolution light pattering tool is reported. Essential experimental procedures, results, and advantages of the process are reviewed. The method enables rapid and inexpensive isolation of targeted bacteria showing rare or unique functions from heterogeneous communities or populations. This…

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Surgical Techniques to Optimize Ovarian Reserve during Laparoscopic Cystectomy for Ovarian Endometrioma

This protocol presents techniques to laparoscopically excise ovarian endometrioma, to perform adhesiolysis with sparing electrosurgical application, and to employ intraoperative chromopertubation to assess for genital tract patency. This systematic approach will facilitate optimal endometriosis management, guide concomitant adnexal surgeries, and enhance post-surgical fertility outcomes. Surgical techniques to optimize ovarian reserve…

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Predicting sepsis severity at first clinical presentation: The role of endotypes and mechanistic signatures

Summary Background Inter-individual variability during sepsis limits appropriate triage of patients. Identifying, at first clinical presentation, gene expression signatures that predict subsequent severity will allow clinicians to identify the most at-risk groups of patients and enable appropriate antibiotic use. Methods Blood RNA-Seq and clinical data were collected from 348 patients…

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Gene pulser bio rad manual

Gene pulser bio rad manual Bio-Rad Gene Pulser MXcell™ Electroporation System An efficient method for markerless mutant generation by Leishmania donovani: proteasome-mediated down-regulation Visit us on the Web at discover.bio-rad.com Bio-Plex,Leading Life Science Discovery Advance your understanding of cell biology and…

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MemVerge Big Memory Technology Speeds Genomic Analytic Processes for Penn State Huck Institutes of the Life Sciences

MemVerge Memory Machine Dramatically Cuts Genome Sequencing Processes for Plant DNA Analytics to Help Develop More Sustainable Plant Products MILPITAS, Calif., Sept. 21, 2021 /PRNewswire/ — MemVerge®, the pioneers of Big Memory software, today announced Memory Machine™ Big Memory virtualization software has been selected by Penn State Huck Institutes of…

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Bronx Serviceman, Killed During the Korean War, Accounted for 70 Years Later

By SÍLE MOLONEY The U.S. Defense Prisoner of War (POW) / Missing in Action (MIA) Accounting Agency (DPAA) announced on Monday, Sept. 20, that U.S. Army 1st Lt. Anthony R. Mazzulla, 26, of The Bronx, New York, killed during the Korean War, was accounted for on May 28, 2020….

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IRE combined with toripalimab versus IRE alone for LAPC

Introduction Pancreatic ductal adenocarcinoma (PDAC) is a lethal gastrointestinal disease with increasing morbidity, which also has a growing impact on cancer-specific mortality worldwide.1 Nearly 40% of all PDAC cases are localized to the pancreas and characterized with the involvement of major vascular structures, leading to unresectable disease without metastases detected…

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Sports Field Breeding Gets a Boost

University of Oklahoma Gaylord Family Oklahoma Memorial Stadium, located in Norman, Oklahoma. Source: Pure Seed Mowing down the competition takes effort, but breeding turfgrass for athletic fields brings a new, creative challenge to the industry. Attitudes about the appropriate playing surface for athletic fields have evolved in recent years, resulting…

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Jobs That Are Projected to Grow the Fastest From 2020 to 2030

More about BLS’ employment projections and the above ranking All the above jobs are projected to grow faster than 7.7%, the projected total employment growth from 2020 to 2030 according to the Bureau of Labor Statistics (BLS). In fact, the above jobs are all projected to see employment percent increases…

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New research provides an additional overview of optimistic reasons related to heart regeneration

CRISPR / Cas9 Wls knockdown in cardiomyocytes promotes cardiac fibroblast proliferation during neonatal cardiac regeneration. Green indicates vimentin-positive cardiac fibroblasts. Red indicates cells that are proliferating with the EdU label. Blue indicates nuclei with DAPI staining. Credit: Texas Heart Institute Texas Heart Institute has new research from its abundant cardiomyocyte…

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PacBio sequencing output increased through uniform and directional fivefold concatenation

Strategy and design of the method We sought to develop a simple method to increase the sequencing capability of PacBio CCS to sequence several diverse DNA libraries ~ 870 bp in length that encoded protein variants originating from a directed evolution campaign. To achieve an increase in the throughput of a PacBio sequencing…

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Korea’s President Moon announces support for venture firms & more news

South Korea’s aspiration to become a tech and startup powerhouse got a strong endorsement as President Moon Jae-in announced a special fund worth 1 trillion won (US$850 million) at the ‘K + Venture’ (K Adventure) event. Also, in the news, US venture firm 500 startups announce its ‘Seed program,’ Korean…

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High-purity production and precise editing of DNA base editing ribonucleoproteins

Abstract Ribonucleoprotein (RNP) complex–mediated base editing is expected to be greatly beneficial because of its reduced off-target effects compared to plasmid- or viral vector–mediated gene editing, especially in therapeutic applications. However, production of recombinant cytosine base editors (CBEs) or adenine base editors (ABEs) with ample yield and high purity in…

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Multiform antimicrobial resistance from a metabolic mutation

Abstract A critical challenge for microbiology and medicine is how to cure infections by bacteria that survive antibiotic treatment by persistence or tolerance. Seeking mechanisms behind such high survival, we developed a forward-genetic method for efficient isolation of high-survival mutants in any culturable bacterial species. We found that perturbation of…

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How to Protect Critical Federal IT Assets at the Peak of Storm Season

While the 2021 hurricane season has already seen a flurry of activity, the worst might still be to come. Expectations for an above average storm season have increased to 65 percent according to the latest forecast from the National Oceanic and Atmospheric Administration, with peak season typically taking shape between mid-August and…

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Having issue running TransposonPSI

Having issue running TransposonPSI 0 Hi, I have tried running the transposonPSI on our sever several times and always come back with an error message below. Is there anyone that knows how this can be resolved. Your help and contribution would be much appreciated. (base) taiwo@zetaadmin:/home/programs/repeatmasker/TransposonPSI_08222010$ sudo ./transposonPSI.pl /home/taiwo/Genome_Annotations/funannotate_E34/genome.proteins.fasta prot…

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Infective endocarditis caused by ESBL-EC

Tsuneaki Kenzaka,1,2 Yuto Shinkura,1 Shizuo Kayama,3– 5 Liansheng Yu,3– 5 Sayoko Kawakami,3 Motoyuki Sugai,3– 5 Satoru Kawasaki1 1Department of Internal Medicine, Hyogo Prefectural Tamba Medical Center, Tanba, Japan; 2Division of Community Medicine and Career Development, Kobe University Graduate School of Medicine, Kobe, Japan; 3Antimicrobial Resistance Research Center, National Institute of…

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Helicobacter pylori in Pakistan | IDR

Introduction Helicobacter pylori, an infectious agent of human chronic active gastritis and also associated with other gastric ailments such as peptic ulcer, gastric cancer, and mucosa-associated lymphoid tissue lymphoma.1 Clarithromycin is considered the best option among other antibiotics for the management of this bacterial infection.2 However, increasing resistance against this…

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Sensex climbs 236 pts; breadth remains weak

The benchmark indices firmed up further in early afternoon trade. The Nifty regained the 16,500 mark. Realty shares extended recent losses. At 12:20 IST, the barometer index, the S&P BSE Sensex, surged 236.29 points or 0.43% at 55,565.61. The Nifty 50 index added 53.30 points or 0.32% at…

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Irreversible Electroporation for Patients with Localised Prostate Cancer: Expert Opinion on this Versatile Therapeutic Approach

Interviewees: Olivier Cussenot,1 Phillip Stricker2,3,4 1. Department of Urology, APHP-Sorbonne University, Tenon Hospital, Paris, France2. Department of Urology, St Vincent’s Private Hospital and Clinic, Sydney, Australia3. Garvan Institute of Medical Research, Sydney, Australia4. Australian PC Centre, Sydney, Australia Disclosure: Cussenot is an expert for INCa (French National Cancer Institute) and…

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News and Insights | Nasdaq

Now Playing China Will Have Difficult Second Half: UBS’s Zuercher 1 day ago Now Playing In the Money: Can’t Stop, Won’t Stop 2 days ago Now Playing Biden Aims for 50% Clean Car Goal for U.S. by 2030 2 days ago Now Playing Delta Cases Threaten China Recovery 3 days…

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