Tag: RFP
Nanobody-based RFP-dependent Cre recombinase for selective anterograde tracing in RFP-expressing transgenic animals
Screening of efficient mCherry-binding protein (MBP) pairs to design Cre recombinase dependent on RFPs First, we aimed to construct Cre recombinase dependent on RFPs based on the reported tool named Cre-DOG30. In this system, N-terminal and C-terminal split Cre recombinase fragments are fused with specific nanobodies for target proteins, and…
illumina miseq reagent Bids, RFP & Government Contracts
Reagent (Chemistry) Kits Quick View Type : Bid Notification Due : 04 Aug, 2017 (about 4 years ago) Posted : about 4 years ago Started : 28 Jul, 2017 (about 4 years ago) All of which require Reagent (Chemistry) Kits for data analysis as follows:• Sequencing by Synthesis (SBS) Kits•…
Establishment and Genetic Manipulation of Murine Hepatocyte Organoids
A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering. The liver is the biggest organ in mammals. It plays very important role in metabolism and detoxification. Although…
Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model. So this protocol enables us to induce pathological phase transitions of TDP-43 and address their cellular consequence in the spinal motor neurons in vivo,…
AAV ShRNA Cloning Service – CD Biospeeds
AAV ShRNA Cloning Service AAV ShRNA Cloning Service Adeno-associated virus (AAV) is a type of parvovirus. Its genome is single-stranded DNA and has the ability to infect both dividing and non-dividing cells. Adenovirus or herpes virus is usually needed to help it replicate and expand in the…
Ttc30a affects tubulin modifications in a model for ciliary chondrodysplasia with polycystic kidney disease
Significance Cilia are tubulin-based cellular appendages, and their dysfunction has been linked to a variety of genetic diseases. Ciliary chondrodysplasia is one such condition that can co-occur with cystic kidney disease and other organ manifestations. We modeled skeletal ciliopathies by mutating two established disease genes in Xenopus tropicalis frogs. Bioinformatic…
Can I remove the control in differential expression analysis?
Hi there, Essentially, my experimental design is control vs treatment. Cells were sorted based on fluorescence, so there are 4 different “colors” of treated cells, i.e. red, green, green+red, and blue+green+red. I am interested in how the colors differ from one another. And, I have duplicates for all colors and…
