Tag: RFP

Animals All experimental procedures were performed in compliance with animal protocols approved by the Institutional Animal Care and Use Committee at Boston Children’s Hospital (Protocol no. 20-05-4165 R). Mice were provided with food and water ad libitum, housed on a 12-hour light/dark schedule (7 a.m.–7 p.m. light period) with no more than five mice…

Reduced Kif13b expression increases the ratio of neurons to progenitors in the developing rat brain To compare the roles of Kif13B vs. Kif1A in rat brain cortical development, we injected embryonic day 16 (E16) rat embryos in utero with an empty GFP control plasmid, or shRNAs designed for knockdown of…

– RFP Status –ActiveExpiredAll – Response Submission Type –AllDigital RFP SubmissionPhysical RFP SubmissionBoth (Physical or Digital) – Select Country –USACanadaIndiaAfghanistanAlbaniaAlgeriaAndorraAngolaAntigua and BarbudaArgentinaArmeniaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBhutanBoliviaBosnia and HerzegovinaBotswanaBrazilBrunei BulgariaBurkina FasoBurundiCabo VerdeCambodiaCameroonCentral African RepublicChadChileChinaColombiaComorosCongo, Democratic Republic of Congo, Republic of theCosta RicaCote d’IvoireCroatiaCubaCyprus Czech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFijiFinlandFranceGabonGambiaGeorgiaGermanyGhanaGreeceGrenadaGuatemalaGuineaGuinea-BissauGuyanaHaitiHondurasHungaryIcelandIndonesiaIranIraqIrelandIsraelItalyJamaicaJapanJordanKazakhstanKenyaKiribatiKosovoKuwaitKyrgyzstanLaosLatviaLebanonLesothoLiberiaLibya LiechtensteinLithuaniaLuxembourgMacedoniaMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMauritaniaMauritiusMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMoroccoMozambiqueMyanmar (Burma)NamibiaNauruNepalNetherlandsNew ZealandNicaraguaNigerNigeriaNorth KoreaNorwayOmanPakistanPalauPalestinePanamaPapua New GuineaParaguayPeruPhilippinesPolandPortugalQatarRomaniaRussiaRwandaSamoaSan…

Animal models All procedures involving animals were approved by the Institutional Animal Care and Use Committee (IACUC) at Washington University in St. Louis, MO. Veterinary care and housing was provided by the veterinarians and veterinary technicians of Washington University School of Medicine under Dougherty lab’s approved IACUC protocol. All protocols…

The Space Force next year will select launch providers for Phase 3 of the National Security Space Launch (NSSL) program. Industry proposals are due Dec. 15. This next phase of NSSL marks a significant evolution in how the Space Force approaches launch, leaning into the commercial market in a way…

The need for new approaches to study membrane contact sites (MCSs) has grown due to increasing interest in studying these cellular structures and their components. Here, we present a protocol that integrates previously available microscopy technologies to identify and quantify intra-organelle and inter-organelle protein complexes that reside at MCSs. Our…

– RFP Status –ActiveExpiredAll – Response Submission Type –AllDigital RFP SubmissionPhysical RFP SubmissionBoth (Physical or Digital) – Select Country –USACanadaIndiaAfghanistanAlbaniaAlgeriaAndorraAngolaAntigua and BarbudaArgentinaArmeniaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBhutanBoliviaBosnia and HerzegovinaBotswanaBrazilBrunei BulgariaBurkina FasoBurundiCabo VerdeCambodiaCameroonCentral African RepublicChadChileChinaColombiaComorosCongo, Democratic Republic of Congo, Republic of theCosta RicaCote d’IvoireCroatiaCubaCyprus Czech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFijiFinlandFranceGabonGambiaGeorgiaGermanyGhanaGreeceGrenadaGuatemalaGuineaGuinea-BissauGuyanaHaitiHondurasHungaryIcelandIndonesiaIranIraqIrelandIsraelItalyJamaicaJapanJordanKazakhstanKenyaKiribatiKosovoKuwaitKyrgyzstanLaosLatviaLebanonLesothoLiberiaLibya LiechtensteinLithuaniaLuxembourgMacedoniaMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMauritaniaMauritiusMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMoroccoMozambiqueMyanmar (Burma)NamibiaNauruNepalNetherlandsNew ZealandNicaraguaNigerNigeriaNorth KoreaNorwayOmanPakistanPalauPalestinePanamaPapua New GuineaParaguayPeruPhilippinesPolandPortugalQatarRomaniaRussiaRwandaSamoaSan…

– RFP Status –ActiveExpiredAll – Response Submission Type –AllDigital RFP SubmissionPhysical RFP SubmissionBoth (Physical or Digital) – Select Country –USACanadaIndiaAfghanistanAlbaniaAlgeriaAndorraAngolaAntigua and BarbudaArgentinaArmeniaAustraliaAustriaAzerbaijanBahamasBahrainBangladeshBarbadosBelarusBelgiumBelizeBeninBhutanBoliviaBosnia and HerzegovinaBotswanaBrazilBrunei BulgariaBurkina FasoBurundiCabo VerdeCambodiaCameroonCentral African RepublicChadChileChinaColombiaComorosCongo, Democratic Republic of Congo, Republic of theCosta RicaCote d’IvoireCroatiaCubaCyprus Czech RepublicDenmarkDjiboutiDominicaDominican RepublicEcuadorEgyptEl SalvadorEquatorial GuineaEritreaEstoniaEthiopiaFijiFinlandFranceGabonGambiaGeorgiaGermanyGhanaGreeceGrenadaGuatemalaGuineaGuinea-BissauGuyanaHaitiHondurasHungaryIcelandIndonesiaIranIraqIrelandIsraelItalyJamaicaJapanJordanKazakhstanKenyaKiribatiKosovoKuwaitKyrgyzstanLaosLatviaLebanonLesothoLiberiaLibya LiechtensteinLithuaniaLuxembourgMacedoniaMadagascarMalawiMalaysiaMaldivesMaliMaltaMarshall IslandsMauritaniaMauritiusMexicoMicronesiaMoldovaMonacoMongoliaMontenegroMoroccoMozambiqueMyanmar (Burma)NamibiaNauruNepalNetherlandsNew ZealandNicaraguaNigerNigeriaNorth KoreaNorwayOmanPakistanPalauPalestinePanamaPapua New GuineaParaguayPeruPhilippinesPolandPortugalQatarRomaniaRussiaRwandaSamoaSan…

As technology continues its exponential growth, Google is riding the forefront wave, constantly innovating and refining. Its most recent announcement has the tech world buzzing: the integration of generative AI into Search. In this exploration of Google Generative AI features we dive deep and underline its transformative potential for search…

Cover Image On the cover: Immunofluorescence image of the pancreatic islet from Pdx1 βKO mice detected with anti-insulin antibody (blue). The EGFP- and RFP-positive cells show the effective knockout of Pdx1 without insulin staining. Image courtesy of Kyosei Ueki, Yuya Nishida, and Hirotaka Watada, Department of Metabolism and Endocrinology, Juntendo…

The Space Force yesterday posted the final request for proposals for Phase 3 of the space launch acquisition program, a dual program approach with two separate contract types. Earlier this year, the service announced it would be using a dual-lane approach — Lane 1 and Lane 2 — for the…

WASHINGTON — The U.S. Space Force on Oct. 4 released two solicitations for the next round of national security launch contracts, known as National Security Space Launch Phase 3. NSSL Phase 3 is a multibillion-dollar procurement of launch services projected for 2025 through 2034. United Launch Alliance and SpaceX won…

Introduction The primary cilium (9 + 0 architecture) is a microtubule-based immotile sensory organelle in vertebrates (Tucker et al, 1979). Enormous progress has been made in the past several decades concerning our understanding of the structure and functions of the primary cilium (Sung & Leroux, 2013). The subdomain between the…

Don’t miss out on our upcoming webinar, “Build an RFP Chatbot with AI Using Your Old RFPs,” and discover how to easily manage security questionnaires and Requests for Proposals (RFPs). By integrating Decisions with Google Vertex AI, you’ll learn how to extract relevant information from your security documents and quickly…

Modular toolkit structure – The toolkit consists of seven modules for quick and easy assembly of integrative constructs and Cas9-helper plasmids. Lvl0 Module: single parts in entry vectors. Exp Module: assembly of overexpression constructs. Pro Module: assembly and screening of new promoters. Del Module: assembly of disruption constructs. Int Module:…

Hyderabad, India (Metro Rail Today): Hyderabad Airport Metro Limited (HAML) is engaged in the development of Mass Rapid Transit System (MRTS) in the city of Hyderabad. As a part of this endeavor, HAML intends to prepare Preliminary Project Reports (PPRs) and Detailed Project Reports (DPRs) for development Mass Rapid Transit System…

Hyderabad Metro Phase 3: Tenders invited for selection of consultants for PPRs, DPRs. (Photo: Wikipedia) Hyderabad Metro Phase-3: Expansion gains momentum as consultants sought for vital project reports, aiming to reshape city’s transit landscape. In a significant move towards the expansion of Metro Rail corridors in Phase-III, the Telangana Government’s…

Creating custom GTF file for use with Cellranger with barcode sequences 0 Hello all I am working with 3′ single cell expression data that has been generated on the 10x Chromium platform. The starting material is human cells that have been labeled with an RFP expressing lentiviral vector which also…

Introduction Tuberculosis is a threat to human health caused by Mycobacterium tuberculosis that affected lung and other extra-pulmonary sites. There was an estimated 10.0 million people fell ill with TB, 1.2 million TB deaths among HIV-negative people, and an additional 251,000 deaths among HIV-positive people in 2018 (World Health Organization,…

NEW YORK – Akribion Genomics, currently a brand of German biotechnology company Brain Biotech, has developed new families of CRISPR nucleases that the group says will have applications in research, diagnostics, and therapeutics. Dubbed G-dases, the novel CRISPR-associated nucleases include two classes: G-dase M and G-dase E. While G-dase M…

Generative artificial intelligence may have captured the attention of the media industry because of its potential to reshape the landscapes of search and content creation, but so far those applications remain largely hypothetical. However, starting earlier this year a number of publishers began using generative AI to streamline their back-end…

1. Introduction Tuberculosis (TB) is an infectious disease caused by Mycobacterium tuberculosis (MTB). According to the “Global Tuberculosis Report” published by the World Health Organization (WHO) in 2022, 10.6 million new cases of TB worldwide were estimated in 2021, and 1.4 million people died from the disease. That year, 3.6%…

WASHINGTON — United Launch Alliance CEO Tory Bruno said he has “a bunch of questions” about the latest changes announced by the Space Force for the procurement of national security space launch services. Speaking on the CNBC “Manifest Space with Morgan Brennan” podcast that aired July 27, Bruno was asked…

Introduction Persisters are assumed to be defined as non-replicating or slow-growing bacteria that are not killed by antibiotics, which can return to a growth state upon removal of antibiotics, and are sensitive to the same antibiotics.1 Persisters play an important role in medicine pose a major challenge to the treatment…

To provide structure and clarity to the understanding of the intricate control mechanism herein investigated, we have deconstructed intracellular FGFR2 to the three principle regulatory regions: JM, KD and CT. This provides an understanding of how both JM and CT influence KD activation and dimerization. Through subsequent reconstruction of their…

– Advertisement – The U.S. Space Force has released a revised draft solicitation for National Security Space Launch Phase 3, increasing the number of heavy-lift launch providers from two to three. The procurement of launch services is projected for 2025-2029. The Space Systems Command is seeking industry feedback before releasing…

ADVERTISEMENT Space Systems Command releases National Security Space Launch Phase 3 Draft RFP by Brad Bartz Los Angeles CA (SPX) Jul 14, 2023 In a recent announcement, the Space Systems Command (SSC) reported that it has issued a second round of draft Requests for Proposals (RFPs) as part…

WASHINGTON — The U.S. Space Force on July 13 released a revised draft solicitation for the next round of national security launch contracts, known as National Security Space Launch Phase 3. In a major departure from the first draft request for proposals released in February, the Space Force is increasing…

Space Systems Command yesterday posted a second draft request for proposals for Phase III of the space launch acquisitions program, a dual-lane approach with two separate contract types. In February, Inside Defense reported the Space Force would use dual lanes for National Security Space Launch services to widen the scope of contractors’ competition for future…

Flow cytometers, droplet dispensers, and limiting dilution techniques aimed at depositing a single iPSC in a well, often create a strenuous and unnatural environment that leads to low clonal survival efficiency.    The CellRaft® Array provides a more biologically relevant culture environment by offering flask-like culture conditions combined with single-cell…

The process of gene editing involves the transformation of a substantial number of cells, followed by the isolation of individual cells from the larger population to establish clonal colonies.   Due to the significant number of gene edits required in modern research and the labor-intensive workflow, there is a pressing…

Molecular biology A two-color intramolecular human SERCA2a (2CS) biosensor, based on human cardiac SERCA2a fused to green fluorescent protein (eGFP) and red fluorescent protein (tagRFP) was developed to detect structural changes that are related to the functional changes of SERCA20,22,25. Briefly, tagRFP was genetically fused to the N-terminus of SERCA…

would have cloned the gene for red fluorescent protein RFP DsRed2 into the plasmidpUC19 and transformedE. coli DH5α.After selecting for clones, we would have isolated the proteins from these clones and the clonesisolated from the transformation experiment (Lab 8,E. coli that had the pGLO plasmid).A Western blot will be performed…

you decide you want to extract the pUC19 plasmid with the RFP gene to use in an upcomingexperiment.Your benchmate set up the overnight culture for you.They inoculated the clones in LB + IPTG.You took the cloudy culture and spun the cells down.You perform the plasmid extract and get a low…

Babesia genomes lack enzymes of the complete haem synthesis system as compared to Plasmodium genomes Babesia genomes comprise four chromosomes, and range in size from 6 to 15 Mb [e.g. Babesia microti (6.44 Mb), Babesia bovis (8.18 Mb), Babesia bigemina (12.84 Mb), Babesia ovata (14.45 Mb), Babesia ovis (8.38 Mb) and Babesia divergens (9.65 Mb) (Additional file…

Mapping of bulk RNA-seq data and differential gene expression (DGE) Using the splice-aware aligner STAR, we mapped the RNA-seq reads to the H. bakeri genome assembly obtained from WormBase ParaSite (PRJEB15396). Among all the datasets, 93.26–95.62% of the reads uniquely mapped to the reference genome (Table 1), reflecting the high…

The growing demand for more rapid high-throughput cell-line development and improved screening in the pharmaceutical space is motivating research facilities and companies to automate their labs. Though robotics has been present in some laboratories for around four decades, it is becoming increasingly common. It helps to free researchers of repetitive…

Molecular profiling reveals common changes in matched pairs of HNSCC primary tumor and metastasis-derived cell lines Identification of both genetic and functional differences between primary and metastatic variants of HNSCC would be enabled by in vitro models derived independently from these sites from the same patient. Unfortunately, HNSCC tumor cells…

. 2023 Apr 20;14:1179394. doi: 10.3389/fpls.2023.1179394. eCollection 2023. Affiliations Expand Affiliation 1 College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China. Item in Clipboard Shanwen Ye et al. Front Plant Sci. 2023. Show details Display options Display options Format AbstractPubMedPMID . 2023 Apr 20;14:1179394. doi: 10.3389/fpls.2023.1179394. eCollection 2023. Affiliation 1…

Bba_K4501010 Device rgasra Pau Contains exosome booster genes such as STEAP3, SDC4 and NadB and a puromycin resistance gene for selection. BBa_K4501012 Composite Exosome booster 2 Eva Contains exosome booster gene nSMase and a hygromycin resistance gene for selection. BBa_K4501013 Composite CD63_His-tag Jaume Contains the tetraspannin CD63 labelled with 6x…

In the fiercely competitive space launch industry, United Launch Alliance and SpaceX have established themselves as the reigning providers of U.S. national security launch services, leaving little room for potential rivals to challenge their dominance. A SpaceX Falcon 9 rocket on Jan. 18, 2023, lifted off from Space Launch Complex…

The town of Sylvan Lake decided to table the decision to award the tender of $570,000 for the Pogadl Park Tournament House to IMC Construction Ltd. during the meeting on March 27. Town staff told Sylvan Lake News the request for proposal (RFP) was posted to the town’s procurement website…

Genetic screen identifies modulators of Tun-mediated neuronal death To identify genes that ameliorate neuronal cell death caused by Tun-induced ER stress, we performed a loss-of-function pooled genetic screen using our druggable genome sgRNAs library. We first generated a clonal iPSC line that expresses Cas9 under the GAPDH promoter (Fig. 1A). We…

ANKLE1 is the causal gene for breast and ovarian cancer risk in the chr19p13.1 region Expression quantitative trait loci (eQTL) data have revolutionized how geneticists identify candidate causal genes from genome-wide association study (GWAS) loci. We integrated the most recent meta-analysis of breast cancer GWAS10 and Genotype-Tissue Expression (GTEx) project…

Photo Contributor: Gorodenkoff/Shutterstock The U.S. Space Force has released a pair of draft requests for proposals to describe a competitive “dual-lane” procurement approach for the third phase of the National Security Space Launch program. Space Systems Command, USSF’s technology development and acquisition arm, will provide NSSL Phase 3 details at…

CRISPR/Cas9 allows manipulation of A. castellanii. a Constructs used to constitutively express Cas9 in A. castellanii. The sequence of the gRNA is depicted with diamonds followed by the Cas9-binding scaffold and an A. castellanii tRNA (yellow rectangles). The localization of the GFP-Cas9 fusion for the different constructs is shown by…

Animals B6N mice, ICR (CD1) mice, SD rats, and LE rats were obtained from The Jackson Laboratory Japan (Yokohama, Japan). LH rats (No. 0873) were obtained from the National Bio Resource Project for the Rat (NBRP-Rat, Kyoto, Japan). ELKS conditional KO mouse was made floxed ELKS mouse crossed with E3N-Cre…

Hello, I would like to use ORFik to determine the coverage of the different ORFs across the maize genome. I have ribo-seq data, the latest annotation file (a GFF3), and the v5 genome fasta file for B73. After running my code, three Large CompressedGRangesLists are created and none of them…

David versus Goliath: a classic tale as relevant today as it was ages ago, and one which is evergreen within the ecommerce space, where small-to-medium businesses (SMBs) battle the odds of their larger competitors every single day. Like any good battle, though, the future is not written in stone. The…

Viral strains utilized in this work The following viral strains have been used in this study: Pandoravirus neocaledonia3, Pandoravirus macleodensis3, Pandoravirus kuranda (this study, ON887157), Mollivirus kamchatka26, Pithovirus sibericum27 and Mimivirus reunion28. Cloning of DNA constructs All primers used in this study are listed in Table S1. All vectors used in…

Screening of efficient mCherry-binding protein (MBP) pairs to design Cre recombinase dependent on RFPs First, we aimed to construct Cre recombinase dependent on RFPs based on the reported tool named Cre-DOG30. In this system, N-terminal and C-terminal split Cre recombinase fragments are fused with specific nanobodies for target proteins, and…

Reagent (Chemistry) Kits Quick View Type : Bid Notification Due : 04 Aug, 2017 (about 4 years ago) Posted : about 4 years ago Started : 28 Jul, 2017 (about 4 years ago) All of which require Reagent (Chemistry) Kits for data analysis as follows:• Sequencing by Synthesis (SBS) Kits•…

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering. The liver is the biggest organ in mammals. It plays very important role in metabolism and detoxification. Although…

We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model. So this protocol enables us to induce pathological phase transitions of TDP-43 and address their cellular consequence in the spinal motor neurons in vivo,…

AAV ShRNA Cloning Service AAV ShRNA Cloning Service Adeno-associated virus (AAV) is a type of parvovirus. Its genome is single-stranded DNA and has the ability to infect both dividing and non-dividing cells. Adenovirus or herpes virus is usually needed to help it replicate and expand in the…

Significance Cilia are tubulin-based cellular appendages, and their dysfunction has been linked to a variety of genetic diseases. Ciliary chondrodysplasia is one such condition that can co-occur with cystic kidney disease and other organ manifestations. We modeled skeletal ciliopathies by mutating two established disease genes in Xenopus tropicalis frogs. Bioinformatic…

Hi there, Essentially, my experimental design is control vs treatment. Cells were sorted based on fluorescence, so there are 4 different “colors” of treated cells, i.e. red, green, green+red, and blue+green+red. I am interested in how the colors differ from one another. And, I have duplicates for all colors and…