Tag: RFP

Babesia genomes lack enzymes of the complete haem synthesis system as compared to Plasmodium genomes Babesia genomes comprise four chromosomes, and range in size from 6 to 15 Mb [e.g. Babesia microti (6.44 Mb), Babesia bovis (8.18 Mb), Babesia bigemina (12.84 Mb), Babesia ovata (14.45 Mb), Babesia ovis (8.38 Mb) and Babesia divergens (9.65 Mb) (Additional file…

Mapping of bulk RNA-seq data and differential gene expression (DGE) Using the splice-aware aligner STAR, we mapped the RNA-seq reads to the H. bakeri genome assembly obtained from WormBase ParaSite (PRJEB15396). Among all the datasets, 93.26–95.62% of the reads uniquely mapped to the reference genome (Table 1), reflecting the high…

The growing demand for more rapid high-throughput cell-line development and improved screening in the pharmaceutical space is motivating research facilities and companies to automate their labs. Though robotics has been present in some laboratories for around four decades, it is becoming increasingly common. It helps to free researchers of repetitive…

Molecular profiling reveals common changes in matched pairs of HNSCC primary tumor and metastasis-derived cell lines Identification of both genetic and functional differences between primary and metastatic variants of HNSCC would be enabled by in vitro models derived independently from these sites from the same patient. Unfortunately, HNSCC tumor cells…

. 2023 Apr 20;14:1179394. doi: 10.3389/fpls.2023.1179394. eCollection 2023. Affiliations Expand Affiliation 1 College of Forestry, Fujian Agriculture and Forestry University, Fuzhou, China. Item in Clipboard Shanwen Ye et al. Front Plant Sci. 2023. Show details Display options Display options Format AbstractPubMedPMID . 2023 Apr 20;14:1179394. doi: 10.3389/fpls.2023.1179394. eCollection 2023. Affiliation 1…

Bba_K4501010 Device rgasra Pau Contains exosome booster genes such as STEAP3, SDC4 and NadB and a puromycin resistance gene for selection. BBa_K4501012 Composite Exosome booster 2 Eva Contains exosome booster gene nSMase and a hygromycin resistance gene for selection. BBa_K4501013 Composite CD63_His-tag Jaume Contains the tetraspannin CD63 labelled with 6x…

In the fiercely competitive space launch industry, United Launch Alliance and SpaceX have established themselves as the reigning providers of U.S. national security launch services, leaving little room for potential rivals to challenge their dominance. A SpaceX Falcon 9 rocket on Jan. 18, 2023, lifted off from Space Launch Complex…

The town of Sylvan Lake decided to table the decision to award the tender of $570,000 for the Pogadl Park Tournament House to IMC Construction Ltd. during the meeting on March 27. Town staff told Sylvan Lake News the request for proposal (RFP) was posted to the town’s procurement website…

Genetic screen identifies modulators of Tun-mediated neuronal death To identify genes that ameliorate neuronal cell death caused by Tun-induced ER stress, we performed a loss-of-function pooled genetic screen using our druggable genome sgRNAs library. We first generated a clonal iPSC line that expresses Cas9 under the GAPDH promoter (Fig. 1A). We…

ANKLE1 is the causal gene for breast and ovarian cancer risk in the chr19p13.1 region Expression quantitative trait loci (eQTL) data have revolutionized how geneticists identify candidate causal genes from genome-wide association study (GWAS) loci. We integrated the most recent meta-analysis of breast cancer GWAS10 and Genotype-Tissue Expression (GTEx) project…

Photo Contributor: Gorodenkoff/Shutterstock The U.S. Space Force has released a pair of draft requests for proposals to describe a competitive “dual-lane” procurement approach for the third phase of the National Security Space Launch program. Space Systems Command, USSF’s technology development and acquisition arm, will provide NSSL Phase 3 details at…

CRISPR/Cas9 allows manipulation of A. castellanii. a Constructs used to constitutively express Cas9 in A. castellanii. The sequence of the gRNA is depicted with diamonds followed by the Cas9-binding scaffold and an A. castellanii tRNA (yellow rectangles). The localization of the GFP-Cas9 fusion for the different constructs is shown by…

Animals B6N mice, ICR (CD1) mice, SD rats, and LE rats were obtained from The Jackson Laboratory Japan (Yokohama, Japan). LH rats (No. 0873) were obtained from the National Bio Resource Project for the Rat (NBRP-Rat, Kyoto, Japan). ELKS conditional KO mouse was made floxed ELKS mouse crossed with E3N-Cre…

Hello, I would like to use ORFik to determine the coverage of the different ORFs across the maize genome. I have ribo-seq data, the latest annotation file (a GFF3), and the v5 genome fasta file for B73. After running my code, three Large CompressedGRangesLists are created and none of them…

David versus Goliath: a classic tale as relevant today as it was ages ago, and one which is evergreen within the ecommerce space, where small-to-medium businesses (SMBs) battle the odds of their larger competitors every single day. Like any good battle, though, the future is not written in stone. The…

Viral strains utilized in this work The following viral strains have been used in this study: Pandoravirus neocaledonia3, Pandoravirus macleodensis3, Pandoravirus kuranda (this study, ON887157), Mollivirus kamchatka26, Pithovirus sibericum27 and Mimivirus reunion28. Cloning of DNA constructs All primers used in this study are listed in Table S1. All vectors used in…

Screening of efficient mCherry-binding protein (MBP) pairs to design Cre recombinase dependent on RFPs First, we aimed to construct Cre recombinase dependent on RFPs based on the reported tool named Cre-DOG30. In this system, N-terminal and C-terminal split Cre recombinase fragments are fused with specific nanobodies for target proteins, and…

Reagent (Chemistry) Kits Quick View Type : Bid Notification Due : 04 Aug, 2017 (about 4 years ago) Posted : about 4 years ago Started : 28 Jul, 2017 (about 4 years ago) All of which require Reagent (Chemistry) Kits for data analysis as follows:• Sequencing by Synthesis (SBS) Kits•…

A long-term ex vitro 3D organoid culture system was established from mouse hepatocytes. These organoids can be passaged and genetically manipulated by lentivirus infection of shRNA/ectopic construction, siRNA transfection and CRISPR-Cas9 engineering. The liver is the biggest organ in mammals. It plays very important role in metabolism and detoxification. Although…

We describe a protocol to induce phase transition of TAR DNA-binding protein 43 (TDP-43) by light in the spinal motor neurons using zebrafish as a model. So this protocol enables us to induce pathological phase transitions of TDP-43 and address their cellular consequence in the spinal motor neurons in vivo,…

AAV ShRNA Cloning Service AAV ShRNA Cloning Service Adeno-associated virus (AAV) is a type of parvovirus. Its genome is single-stranded DNA and has the ability to infect both dividing and non-dividing cells. Adenovirus or herpes virus is usually needed to help it replicate and expand in the…

Significance Cilia are tubulin-based cellular appendages, and their dysfunction has been linked to a variety of genetic diseases. Ciliary chondrodysplasia is one such condition that can co-occur with cystic kidney disease and other organ manifestations. We modeled skeletal ciliopathies by mutating two established disease genes in Xenopus tropicalis frogs. Bioinformatic…

Hi there, Essentially, my experimental design is control vs treatment. Cells were sorted based on fluorescence, so there are 4 different “colors” of treated cells, i.e. red, green, green+red, and blue+green+red. I am interested in how the colors differ from one another. And, I have duplicates for all colors and…