Tag: RNA

When the strands of dsdna are separated this is called?

You are learning about: “When the strands of dsdna are separated this is called?”. This is a “hot” question with 2,820,000 searches/month. Let’s fleetserviceshocrv.com learn more about When the strands of dsdna are separated this is called? in this article. How do scientists name the ends of the DNA strands?…

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Specific High-Sensitivity Enzymatic Molecular Detection System Termed RPA-Based CRISPR-Cas13a for Duck Tembusu Virus Diagnostics

journal contribution posted on 19.05.2022, 07:04 by Dalin He, Gang Liu, Jing Yang, Xiaoning Jiang, Hongzhi Wang, Yaru Fan, Shangyu Gong, Feng Wei, Youxiang Diao, Yi Tang In China, drastic losses in the economy have been caused by the Tembusu virus (TMUV), the causative agent of the egg-drop syndrome, to…

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In vivo hypermutation and continuous evolution

Arnold, F. H. Design by directed evolution. Acc. Chem. Res. 31, 125–131 (1998). Google Scholar  Packer, M. S. & Liu, D. R. Methods for the directed evolution of proteins. Nat. Rev. Genet. 16, 379–394 (2015). Google Scholar  Drake, J. W., Charlesworth, B., Charlesworth, D. & Crow, J. F. Rates of…

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Polygenic Risk Score Performance Improved With Expression-Related Rare Variant Insights

NEW YORK – A team from Stanford University and other centers in the US and China have demonstrated that polygenic risk scores (PRS) based on common variants can be bolstered by incorporating clues from rare variants linked to significant gene expression shifts, dubbed expression outliers. “As individual PRS estimates are…

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Variant #0000255165 (NC_000010.10:g.123278248A>G, FGFR2(NM_000141.4):c.939+1245T>C) – Global Variome shared LOVD

Variant #0000255165 (NC_000010.10:g.123278248A>G, FGFR2(NM_000141.4):c.939+1245T>C) Chromosome 10 Allele Unknown Affects function (as reported) Probably does not affect function Affects function (by curator) Not classified Classification method – Clinical classification likely benign DNA change (genomic) (Relative to hg19 / GRCh37) g.123278248A>G DNA change (hg38) g.121518734A>G Published as FGFR2(NM_022970.3):c.1035T>C (p.Y345=) ISCN – DB-ID FGFR2_000119 Variant remarks VKGL data sharing initiative Nederland Reference – ClinVar ID – dbSNP ID – Origin CLASSIFICATION record Segregation –…

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By keeping the Immune System in good condition, Gut Microbiome protects us from viral infections

The gut microbiota is crucial for health. However, how these non-invasive microbes communicate with the rest of the body to improve host physiology is not fully understood. Researchers from Stockholm, Umea, and Gothenburg Universities, Sweden, claim that the gut microbiota is essential for natural resistance to viral infections. This evidence…

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Single-cell eQTL models reveal dynamic T cell state dependence of disease loci – Nature – Alert Breaking News

Wei, K. et al. Notch signalling drives synovial fibroblast identity and arthritis pathology. Nature 582, 259–264 (2020). ADS CAS PubMed PubMed Central Article Google Scholar  Cembrowski, M. S. & Menon, V. Continuous variation within cell types of the nervous system. Trends Neurosci. 41, 337–348 (2018). CAS PubMed Article Google Scholar  Maurano, M. T. et al. Systematic localization of…

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Dissertations.se: HETEROPLASMY

Showing result 1 – 5 of 7 swedish dissertations containing the word Heteroplasmy. Author : Guilherme Costa Baião; Lisa Klasson; Alistair Darby; Uppsala universitet; []Keywords : NATURAL SCIENCES; NATURVETENSKAP; NATURVETENSKAP; NATURAL SCIENCES; Wolbachia; Drosophila; Drosophila paulistorum; Differential Gene Expression; Reproductive Incompatibility; Reproductive Isolation; Comparative Genomics; Transcriptomics; RNA-Seq; Heteroplasmy; Mitochondria; Genomic…

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A Highly Sensitive and Specific Detection Method for Mycobacterium tuberculosis Fluoroquinolone Resistance Mutations Utilizing the CRISPR-Cas13a System

Objectives CRISPR-Cas13a system-based nucleic acid detection methods are reported to have rapid and sensitive DNA detection. However, the screening strategy for crRNAs that enables CRISPR-Cas13a single-base resolution DNA detection of human pathogens remains unclear. Methods A combined rational design and target mutation-anchoring CRISPR RNA (crRNA) screening strategy was proposed. Results…

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Researchers identify cellular serpins as host factors that restrict SARS-CoV-2 infection

The mass vaccination programs against coronavirus disease 2019 (COVID-19) received massive media attention and were widely credited for reducing the threat of the disease. However, many countries struggle to obtain enough doses to immunize their population, and some immunocompromised individuals are less protected by the vaccines. There is still a…

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WGS Facilitates Gene Editing System Upgrade

Researchers at the Korean Institute of Life Sciences and Technology engineered an efficient, miniaturized CRISPR-Cas gene-editing system that may be more easily packed into vectors for clinical applications. Their system employs the Cas variant Cas12f1 with a guide RNA (gRNA) remodeled to mitigate off-target effects, a design that could potentially…

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Nature Papers on Flying Spider-Monkey Tree Fern Genome, Single-Nucleus Sequencing of Purkinje Cells

The genome of the flying spider-monkey tree fern, Alsophila spinulosa, is reported in Nature Plants this week, providing new insights into tree fern biology. Despite ferns’ high ornamental value and potential as a source of natural products with pharmaceutical applications, little is known about their evolution, with only two small…

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Single-Cell Transcriptome Resource From Human Cell Atlas Team Yields Immune, Disease Insights

NEW YORK – A large international team came up with single-cell and single-nucleus transcriptomic resources to characterize the diverse cell types found in the human body, along with related gene expression and splicing features that offer clues to processes at play within and across tissues and organs. In a series…

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Novel supramolecular CRISPR-Cas9 carrier enables more efficient genome editing — ScienceDaily

Clustered regularly interspaced short palindromic repeats (CRISPR) and their accompanying protein, CRISPR-associated protein 9 (Cas9), made international headlines a few years ago as a game-changing genome editing system. Consisting of Cas9 and strand of genetic material known as a single-guide RNA (sgRNA), the system can target specific regions of DNA…

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Role of CD68 in tumor immunity and prognosis prediction in pan-cancer

Expression of CD68 in pan-cancer First, to fully clarify the expression of CD68 in pan-cancer, we matched the GTEx normal samples with TCGA tumor samples (Fig. 1A). We found that the levels of CD68 were significantly elevated (P < 0.01) in colon adenocarcinoma (COAD), glioblastoma multiforme (GBM), kidney renal clear cell carcinoma (KIRC),…

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Special Issue : Methods and Tools in RNA Biology

A special issue of Non-Coding RNA (ISSN 2311-553X). Deadline for manuscript submissions: 30 November 2022 | Viewed by 9 Special Issue Editors Dr. Mirolyuba Ilieva E-Mail Website Guest Editor Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, DK-2450 Copenhagen SV, DenmarkInterests: non-coding RNAs; stem cell biology; neuroscience Special…

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Norgen 16S rRNA and ITS Library Preparation Kits

Analyze Bacterial and Fungal Microbiomes 16S rRNA Library Preparation Kits Simple and quick workflow. Library could be prepared in less than 4 hours Part of Norgen’s metagenomics workflow: collection, preservation, DNA isolation, sequencing, and analysis. Protocol optimized for DNA isolated from a large range of sample types Up to 384…

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A novel circRNA, circRACGAP1, hampers the progression of systemic lupus erythematosus via miR-22-3p-mediated AKT signalling

This article was originally published here Autoimmunity. 2022 May 11:1-11. doi: 10.1080/08916934.2022.2073590. Online ahead of print. ABSTRACT BACKGROUND: Systemic lupus erythematosus (SLE) is defined as a multisystem autoimmune disease involving various organs, of which exact molecular mechanisms remain elusive. Here, we aimed to investigate a novel circular RNA (circRNA), circRACGAP1,…

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ACADVL – PrimePCR Assay and Template | Life Science

Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting your real-time PCR results. DNA Contamination Control AssayUse the PrimePCR DNA contamination control assay to determine if genomic DNA (gDNA) is present in a sample at a level that may affect PCR…

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RefSeq Release 212 is available!

RefSeq Release 212 is now available online, from the FTP site and through NCBI’s Entrezprogramming utilities, E-utilities. This full release incorporates genomic, transcript, and protein data available as of May 2, 2022, and contains 314,915,153 records, including 229,417,182 proteins, 44,805,833 RNAs, and sequences from 119,373 organisms. The release is provided in several directories…

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Associate Bioinformatics Scientist at Flagship Pioneering, Inc.

Company Summary: Who Are We? Founded by Flagship Pioneering, Empress pioneers the future of small molecule drugs through the discovery of pharmacologic loci. The company’s product platform leverages AI and multi’omic data from thousands of humans around the globe to discover novel, important small molecules inside of humans and their cognate mechanisms…

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Blackrock Clinic launches new device to help combat AF

Electroporation systems being used to tackle a condition which one in four over-55s will develop THE Blackrock Clinic recently started its new program of electroporation for the treatment of patients with atrial fibrillation. Conventional catheter ablation for the treatment of cardiac arrhythmias uses tissue heating (radiofrequency current) to cauterize the…

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Postdoc (m/f/d) – CBIRT

Website Leibniz Institute on Aging – Fritz Lipmann Institute (FLI) Job Category: Bioinformatics Job Job ID: 22/16 Date Posted: 05.05.2022 Deadline: 19.06.2022 Location: Jena, Germany The Leibniz Institute on Aging – Fritz Lipmann Institute (FLI) in Jena is a federal and state government-funded research institute and member…

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Recent developments in miRNA based recombinant protein expression in CHO

Aguiar TQ, Santos SB, Martins IM, Domingues L, Oliveira C (2019) Production and bioengineering of recombinant pharmaceuticals. Proteins: sustainable source, processing and applications. Elsevier, Amsterdam, pp 259–293 Chapter  Google Scholar  Amadi IM, Agrawal V, Christianson T, Bardliving C, Shamlou P, LeBowitz JH (2020) Inhibition of endogenous miR-23a/miR-377 in CHO cells…

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Plant Breeding and CRISPR Plants Market Trends 2022, Global Industry Share, Size, Global Industry Analysis, Key Market Growth Analysis, Segments, Emerging Technologies, Opportunity and Forecast 2027

The “Plant Breeding and CRISPR Plants Market” research report 2021-2027 offering the complete outline of market size, share and growth opportunities, industry segmentation by product type, applications and geographical representation. The report highlights product development along with technological upgrades that can boost the market growth. The Plant Breeding and CRISPR…

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Landscape of helper and regulatory antitumour CD4+ T cells in melanoma

Sallusto, F. & Lanzavecchia, A. Heterogeneity of CD4+ memory T cells: functional modules for tailored immunity. Eur. J. Immunol. 39, 2076–2082 (2009). CAS  PubMed  Article  Google Scholar  Swain, S. L., McKinstry, K. K. & Strutt, T. M. Expanding roles for CD4+ T cells in immunity to viruses. Nat. Rev. Immunol….

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George Church on 4D Omics, Natural Computing, and the BioWeatherMap

By Allison Proffitt May 4, 2022 | George Church opened the 20th Anniversary Bio-IT World Conference & Expo last night, speaking virtually to a packed room at Boston’s Hynes Convention Center while many more attendees tuned in online.  Church, the Robert Winthrop Professor, Genetics, Harvard Medical School, spoke about both…

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Tissue distribution and cancer growth inhibition of magnetic lipoplex-delivered type 1 insulin-like growth factor receptor shRNA in nude mice –

The targeted delivery of therapeutic genes into specific tissues, as well as the determination of the biological fate and potential toxicity of nanoparticles, remains a highly relevant challenge for gene-based therapies. Type 1 insulin-like growth factor receptor (IGF-1R), an important oncogene, is frequently over-expressed in lung cancer and mediates cancer…

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nf-core presentation – HackMD

<!– .slide: data-background=”raw.githubusercontent.com/maxulysse/maxulysse.github.io/main/assets/img/svg/green_white_bg.svg&#8221; –> <a href=”nf-co.re”><img src=”raw.githubusercontent.com/nf-core/logos/master/nf-core-logos/nf-core-logo-darkbg.svg&#8221; width=”60%”></img></a> # What is nf-core\? [Maxime U Garcia](https://maxulysse.github.io/) ▸ [<i class=”fa fa-twitter” aria-hidden=”true”></i>@gau](https://twitter.com/gau/) | [<i class=”fa fa-github” aria-hidden=”true”></i>@maxulysse](https://github.com/maxulysse/) [Karolinska Institutet](https://ki.se/) | [Science for Life Laboratory](https://www.scilifelab.se/) Sweden — ## Overview – 🗄️ [Barntumörbanken](https://ki.se/forskning/barntumorbanken/) – 🧬 [NGI](https://ngisweden.scilifelab.se/) – 🍬 [Nextflow](https://www.nextflow.io/) – 🧙 [nf-core](https://nf-co.re/) — ##…

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Trimming adaptors and primers for RNAseq reads

Trimming adaptors and primers for RNAseq reads 2 Hi all, I have RNA sequencing (sequenced on NextSeq 2000) reads that I know I need to cut the adaptor sequences off of and was planning to use cutadapt to do so. I have identified the adaptor sequence in my reads, but…

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scRNAseq analysis – h5ad file conversion to Seurat format

scRNAseq analysis – h5ad file conversion to Seurat format 0 Hi all. I have a single .h5ad file that contains scRNAseq data from several samples. I would like to convert it so that I can open it in Seurat (I am comfortable with R, but not with Python). I have…

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Environmental Factor – May 2022: Scientific moonshot: Now is the time to sequence RNA, expert says

Rick Woychik, Ph.D., directs NIEHS and the National Toxicology Program. (Image courtesy of NIEHS) Almost two decades have passed since the first sequence of a complete set of DNA was released as part of the Human Genome Project. That initiative expanded understanding of certain cancers, boosted the effectiveness of some…

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Multiplexed genome regulation in vivo with hyper-efficient Cas12a

. 2022 Apr;24(4):590-600. doi: 10.1038/s41556-022-00870-7. Epub 2022 Apr 12. Lucie Y Guo #  1   2 , Jing Bian #  3 , Alexander E Davis  4 , Pingting Liu  4 , Hannah R Kempton  3 , Xiaowei Zhang  3 , Augustine Chemparathy  3 , Baokun Gu  3 , Xueqiu Lin  3 , Draven A Rane  3 , Xiaoshu Xu  3 , Ryan M…

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DataSheet1_Single-Cell RNA-Seq and Bulk RNA-Seq Reveal Intratumoral Heterogeneity and Tumor Microenvironment Characteristics in Diffuse Large B-Cell Lymphoma.docx

Background: Diffuse large B-cell lymphoma (DLBCL) is the most common histologic subtype of non-Hodgkin’s lymphoma (NHL) with highly heterogeneous genetic and phenotypic features. Therefore, a comprehensive understanding of cellular diversity and intratumoral heterogeneity is essential to elucidate the mechanisms driving DLBCL progression and to develop new therapeutic approaches. Methods: We…

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A non-canonical cGAS-STING-PERK pathway facilitates the translational program critical for senescence and organ fibrosis

This article was originally published here Nat Cell Biol. 2022 May 2. doi: 10.1038/s41556-022-00894-z. Online ahead of print. ABSTRACT Innate DNA sensing via the cyclic GMP-AMP synthase-stimulator of interferon genes (cGAS-STING) mechanism surveys microbial invasion and cellular damage and thus participates in various human infectious diseases, autoimmune diseases and cancers….

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tReasure: R-based GUI package analyzing tRNA expression profiles from small RNA sequencing data | BMC Bioinformatics

tReasure (tRNA Expression Analysis Software Utilizing R for Easy use) is a graphical user interface (GUI) tool for the analysis of tRNA expression profiles from deep-sequencing data of small RNAs (small RNA-seq) using R packages. The whole analysis workflow, including the uploading of FASTQ files of small RNA-seq, quantification of…

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EdiGene to Present getools, a Data Analysis Software Toolkit for Advanced Analytics of DNA and RNA Editing Efficiency and Outcomes for Therapeutic Development at the ASGCT 25th Annual Meeting

BEIJING & CAMBRIDGE, Mass.–(BUSINESS WIRE)–BEIJING and CAMBRIDGE, MASS. – (May 2, 2022) – EdiGene, Inc., a global, clinical-stage biotechnology company focused on translating gene-editing technologies into transformative therapies for patients with serious genetic diseases and cancer, will present getools (gene editing data analysis toolkit) for advanced analytics of DNA and…

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Bioinformatics Specialist at The University of Chicago in Chicago, IL – 4091

Job Info Department BSD PED – Section Administrator: Hematology, Oncology, and Stem Cell Transplantation About the Department The Section of Pediatric Hematology/Oncology provides care for patients and families with acute and chronic medical conditions, or other hematologic diseases, requiring the specialty knowledge and skills of Hematology, Oncology and Stem Cell…

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Nature Depth: Where will this revolutionary technology go when artificial intelligence predicts protein structure?

▎ WuXi AppTec content team editor Last July, DeepMind published a study in the journal Nature on artificial intelligence (AI) systems AlphaFold predicting the three-dimensional structure of proteins based on amino acid sequences. The researchers also released the source code of the AI system, making this technology available to scientists…

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AIM brings innovations from labs to hospitals and masses

Human body like the Space is an endless frontier made even more complex by the irreversibility of life by the arrow of time. Similar to the mystery of origin of the Universe some problems in the human body are so complex, for example, the problem of qualia or the Hard…

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CTS Xenon Electroporation System | Thermo Fisher Scientific

The CTS Xenon Electroporation System offers reliable delivery of DNA, RNA, proteins, and other molecules into cells, with exceptional cell viability and recovery. This leads to efficient transfection and genome editing, even in hard-to-transfect cells. A choice of two single-use electroporation chambers of different capacities provides flexibility: the SingleShot chamber…

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Solved 28) What structure below provides an “anticodon”?

Transcribed image text: 28) What structure below provides an “anticodon”? (2pts) OmicroRNA mRNA tRNA rRNA 29) Which of the following modifications is involved in the 3′ (three prime) end-processing of pre- (4pts) mRNA precursors in eukaryotic cells? Addition of a methlyguanosine cap Splicing out introns Addition of Poly-A tail Forming…

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Analysis of circRNA-miRNA-mRNA Regulatory Network in Peripheral Blood of Radiation Workers

The health of radiation workers has always been our focus. Epidemiological investigation shows that long-term exposure to low-dose ionizing radiation can affect human health, especially cancer and cardiovascular disease, and there are many studies on it. However, up to now, there have been few reports on the research of blood…

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Mitogenome-wise codon usage pattern from comparative analysis of the first mitogenome of Blepharipa sp. (Muga uzifly) with other Oestroid flies

Outcome of DNA sequencing, assembly, and validation In this study, initially total DNA was isolated from the finely chopped, full-grown pupa of Blepharipa sp. The NanoDrop spectrophotometer (1294 ng/μl) and the Qubit fluorometer (732.8 ng/μl) both found that the concentration of total DNA in the sample at an optimum level for mitochondrial DNA enrichment. The Tape Station profile showed…

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Annotated file with gene ID (instead of gene symbol)

Annotated file with gene ID (instead of gene symbol) 0 @9cb59de3 Last seen 14 hours ago United States Hello, I am using “featureCounts” in Rsubread package for analyzing bulk RNA-seq of drosophila. Since there is no inbuilt annotations of drosophila, I am using a gtf file in the homepage of…

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PDT001259745.1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Date::03/08/2022 21:07:51 Annotation Method::Best-placed reference protein set; GeneMarkS-2+ Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Provider::NCBI Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Annotation Software revision::2021-01-11.build5132 Genes (total)::5,850 CDSs (total)::5,754 Genes (coding)::5,653 CDSs (with protein)::5,653 Genes (RNA)::96 rRNAs::4, 2, 3 (5S, 16S, 23S) complete rRNAs::4 (5S) partial…

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Circulating cell-free mtDNA release is associated with the activation of cGAS-STING pathway and inflammation in mitochondrial diseases

Craven L, Alston C, Taylor R, Turnbull D (2017) Recent advances in mitochondrial disease. Annu Rev Genomics Hum Genet 18:257–275. doi.org/10.1146/annurev-genom-091416-035426 CAS  Article  PubMed  Google Scholar  El-Hattab AW, Adesina AM, Jones J, Scaglia F (2015) MELAS syndrome: clinical manifestations, pathogenesis, and treatment options. Mol Genet Metab 116(1–2):4–12. doi.org/10.1016/j.ymgme.2015.06.004 CAS  Article …

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biopython – Pipeline for paired end RNA sequence data to proteins

This is a much more complicated question than it might seem. First, you need to understand how RNA-Seq works, and what your data really is. Your “paired-end files” contain reads, which will contain fragments of transcripts. Since those are only fragments of transcripts, you don’t know what frame they are…

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Bioinformatics Scientist in Boston, MA for Dana-Farber Cancer Institute

Details Posted: 27-Apr-22 Location: Boston, Massachusetts Salary: Open Internal Number: 2022-26921 Located in Boston and the surrounding communities, Dana-Farber Cancer Institute is a leader in life changing breakthroughs in cancer research and patient care. We are united in our mission of conquering cancer, HIV/AIDS and related diseases. We strive to…

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How to analyze the differential expression of a gene if it has several ensembl id

How to analyze the differential expression of a gene if it has several ensembl id 0 Hie, I have obtained the differential expressions of genes (from RNA-seq data) in an excel file. I can see one gene has several ensembl ids and I guess the gene is from the haplotype…

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Exploring oncolytic viral therapeutics and immunotherapies derived from DNA and RNA

Due to the high priority and growing need for treatment to alleviate the symptoms associated with potentially fatal prognoses, cancer treatments are continually being developed. Cancer therapies are being developed to prevent malignant cells from forming, proliferating, and metastasizing, as well as assisting in the control of symptoms associated with advanced…

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Finding DEGs from HISAT2/STRINGTIE output

Finding DEGs from HISAT2/STRINGTIE output 0 Hello, I have to search for DEGs from four samples of crop. I am following reference based mapping of reads to genome using HISAT2. I have completed till the generation of merged .gtf files for the samples using STRINGTIE. Since I am new to…

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Study supports use of PharmaJet’s needle-free vaccine delivery system

The PharmaJet Stratis system. [Image from PharmaJet] PharmaJet announced today that study data demonstrated increased immunogenicity when using its needle-free delivery system. Golden, Colorado-based PharmaJet presented the research results for the needle-free vaccine injection technology last week at the World Vaccine Congress. PharmaJet’s needle-free systems have been incorporated into clinical…

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Postdoctoral Scientist – Bioinformatics and Integrative Genomics Research Cluster in , for Cedars-Sinai Medical Center

Details Posted: 26-Apr-22 Location: Nationwide Type: Full-time Salary: Open Internal Number: 20002022 At Cedars-Sinai, postdoctoral scientists and other junior researchers fulfill a critical role in expanding the horizons of medical knowledge through biomedical research. By hosting more than 100 postdocs, who conduct vital research and receive advanced training with expert…

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CRISPR/Cas9 deletions induce adverse on-target genomic effects

Clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) have transformed genome engineering techniques. Numerous toolsets have been created to enable easy and efficient loss-of-function perturbations of functional genomic sites. Study: CRISPR/Cas9 deletions induce adverse on-target genomic effects leading to functional DNA in human cells. Image Credit: elenabsl/Shutterstock The CRISPR/Cas9 system’s elements…

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Genome Editing of Human Primary T Cells With Lipid Nanoparticles

By Reka Geczy, PhD, Aruna Balgi, Stella Park, Rita Zhao, Ethan Watt, Maggie Wong, Cooper Webb, Nikita Jain, PhD, Angela Zhang, PhD, Anitha Thomas, PhD, Samuel Clarke, PhD The expression of the chimeric antigen receptor (CAR) on T cells turns a patient’s cells into cell-based cancer therapies and has revolutionized…

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14 Postdoctoral Fellowship at Umea University, Sweden

Umea University, Sweden invites online Application for various Postdoctoral Fellowship in their different Departments. We are providing a list of Postdoc Fellowship positions available at Umea University, Sweden. Eligible candidate may Apply as soon as possible.   (01) Postdoctoral Fellowship Position Postdoc Fellowship Position summary/title: Postdoctoral fellow – Molecular epidemiology…

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CRISPR Technology Market Size, Share, Demand, Assessment & Opportunity Forecast 2021-2030

Global CRISPR Technology Market Size study, By Product and Service (Hand-Emplaced, Air-Delivered), By Applications (Seismic, Acoustic, Magnetic, Infrared), By End User (Security {Military, Public Security} Critical Infrastructure {Utilities, Industrial & Commercial Facilities}), and Regional Forecasts 2022-2028 Global CRISPR Technology Market is valued approximately USD 1097.66 million in 2021 and is anticipated…

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CRISPR Gene-Editing Market May See a Big Move by 2029

Global Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market Synopsis: The finest Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market research report contains the drivers and restraints for the market that are obtained with the help of SWOT analysis, and also shows all the recent developments, product launches,…

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Hard filtering on GATK HaplotypeCaller giving multiple warnings

I’m using this pipeline for deriving variants from RNA sequencing data: github.com/modupeore/VAP which uses specific versions of various tools, including HaplotypeCaller from GATK (v3.8-0-ge9d806836). The final step is a set of hard filters on the called variants (applied using VariantFilter), but looking at the log files, there are a lot…

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Differential Expression Profiles and Bioinformatics Analysis of tRNA-Derived Small RNAs in Muscle-Invasive Bladder Cancer in a Chinese Population

This article was originally published here Genes (Basel). 2022 Mar 28;13(4):601. doi: 10.3390/genes13040601. ABSTRACT Muscle-invasive bladder cancer (MIBC) leads to a large societal burden. Recently, tRNA-derived small RNAs (tsRNAs), a novel type of noncoding RNA (ncRNAs), have been identified. However, the expression patterns and functions of tsRNAs in MIBC have…

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Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device.

PDF.MPro – Full Text Journal Article: Exploiting the orthogonal CRISPR-Cas12a/Cas13a trans-cleavage for dual-gene virus detection using a handheld device. Home / Search / Abstract 34653714 (Page 1) Advertisement Find full text journal articles PMID: 34653714 (view PubMed database entry)DOI: 10.1016/j.bios.2021.113701 (read at publisher’s website ) Tian Tian, Zhiqiang Qiu, Yongzhong…

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GEO Browser – GEO – NCBI

Filter Mus musculus TSV Brian P. Hermann GSE109033 10x Genonics Drop-seq single-cell RNA-seq of isolated Adult ID4-EGFP mouse spermatogonia, spermatocytes, spermatids & steady-state spermatogenic cells Expression profiling by high throughput sequencing Mus musculus 8 MTX TSV SRA Run Selector Brian P. Hermann Nov 06, 2018 GSE109037 10x Genomics Drop-seq single-cell…

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The nucleolar SUMO-specific protease SENP3 reverses SUMO modification of nucleophosmin and is required for rRNA processing.

The ubiquitin-like SUMO system functions by a cyclic process of modification and demodification, and recent data suggest that the nucleolus is a site of sumoylation-desumoylation cycles. For example, the tumour suppressor ARF stimulates sumoylation of nucleolar proteins. Here, we show that the nucleolar SUMO-specific protease SENP3 is associated with nucleophosmin…

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CircRNA and miRNA expression profiles during remote ischemic postconditioning attenuate brain ischemia/reperfusion injury

. 2022 Apr 19;S0361-9230(22)00098-3. doi: 10.1016/j.brainresbull.2022.04.006. Online ahead of print. Chun-Yan Li  1 , Wei Ma  2 , Kuang-Pin Liu  2 , Jin-Wei Yang  3 , Xian-Bin Wang  2 , Zhen Wu  3 , Tong Zhang  3 , Jia-Wei Wang  3 , Wei Liu  2 , Jie Liu  2 , Yu Liang  2 , Xing-Kui Zhang  2 , Jun-Jun Li  2 , Jian-Hui…

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What is ClustalW alignment?

ClustalW2 is a general purpose DNA or protein multiple sequence alignment program for three or more sequences. For the alignment of two sequences please instead use our pairwise sequence alignment tools. How does protein alignment work? In typical usage, protein alignments use a substitution matrix to assign scores to amino-acid…

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Integrative analysis of eQTL and GWAS summary statistics reveals transcriptomic alteration in Alzheimer brains | BMC Medical Genomics

Significant gene-AD associations With the GWAS summary data from the IGAP and eQTL summary data from BRAINEAC, we performed both SMR and HEIDI tests to estimate the gene-AD associations in three human brain regions: frontal cortex, temporal cortex, and hippocampal regions. For the frontal cortex and hippocampal regions, we obtained…

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The PPR domain of mitochondrial RNA polymerase is an exoribonuclease required for mtDNA replication in Drosophila melanogaster,Nature Cell Biology

Mitochondrial DNA (mtDNA) replication and transcription are of paramount importance to cellular energy metabolism. Mitochondrial RNA polymerase is thought to be the primase for mtDNA replication. However, it is unclear how this enzyme, which normally transcribes long polycistronic RNAs, can produce short RNA oligonucleotides to initiate mtDNA replication. We show…

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Postdoc / Research Scientist in Bioinformatics and Computational Genomics

Job Description Are you a computer geek with a strong interest in genomics? Do you want to use your computational skills to solve human diseases? At the Department of Neurology at Harvard Medical School and Brigham & Women’s Hospital, we have two vacant positions: postdoctoral fellow and research scientist in…

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Science Papers on Cancer Mutational Signatures, System to Deliver CRISRP-Cas9 Machinery Across Blood-Brain Barrier

By sequencing tumors from thousands of cancer patients and comparing them to similar datasets generated by other research groups, a team led by scientists from the University of Cambridge has identified new mutational signatures of cancer, while also uncovering details about organ-specific common and rare signatures. In the study, which…

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03 Postdoctoral Fellowship at European Molecular Biology Laboratory (EMBL), Germany

European Molecular Biology Laboratory (EMBL), Germany invites online Application for various Postdoctoral Fellowship in their different Departments. We are providing a list of Postdoc Fellowship positions available at European Molecular Biology Laboratory (EMBL), Germany. Eligible candidate may Apply as soon as possible.   (01) Postdoctoral Fellowship Position Postdoc Fellowship Position…

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Celsion Corporation Highlighted its PLACCINE Vaccine Platform Technology During Oral … | News

Proprietary, Formulated DNA Plasmid Vaccine Candidate Shows Neutralizing Activity Against 2 Strains of the COVID-19 Virus The Goal to Provide Broader Range Protection within a Highly Flexible Platform Appears to Show Promise LAWRENCEVILLE, N.J., April 21, 2022 (GLOBE NEWSWIRE) — Celsion Corporation (NASDAQ: CLSN), a clinical-stage company focused on DNA-based immunotherapy…

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Endometriosis-related functional modules and hub genes

Introduction Endometriosis (EMS) is a chronic gynecological disease defined as implantation and periodic growth of the endometrial glands and stroma outside the uterine cavity, causing chronic pelvic pain, severe dysmenorrhea, and infertility in 10% reproductive-age women, among which the infertility rate is approximately 30–50%.1,2 Surgical excision is commonly used for…

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Nucleic Acids Research Papers on DAVID Update, ChIP-Atlas, RNA Splicing Assay

Researchers at the Frederick National Laboratory for Cancer Research and the National Institutes of Health describe a 2021 update to the bioinformatics tool DAVID, designed for functional annotation and functional gene enrichment analyses. Along with updates to annotation types and other “Knowledgebase” features, the latest version of the DAVID Gene…

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Metagenomics technology and microbial community diversity analysis methods

A large number of microorganisms in nature cannot be cultivated under laboratory conditions by pure culture methods, and the technical methods of traditional microbiology limit the research on environmental microorganisms. The rapid development of high-throughput omics technology has enabled humans to have an unprecedented understanding of the complex microbial communities…

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Crispr/Cas13a Induced Exponential Amplification for Highly Sensitive and Specific Detection of Circular Rna by Pengfei Song, Pengbo Zhang, Ke Qin, Fengxia Su, Kejian Gao, Xin Liu, Zhengping Li :: SSRN

19 Pages Posted: 23 Mar 2022 See all articles by Pengfei Song University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology…

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Study explores SARS-CoV-2 Omicron variant and infected colon epithelial cells

In a recent study posted to the bioRxiv* preprint server, researchers assessed the impact of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variant on infected human cells and organs. Study: SARS-CoV-2 Omicron BA.1 variant infection of human colon epithelial cells. Image Credit: PHOTOCREO Michal Bednarek/Shutterstock Various studies have…

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RNA Interference (RNAi) Drug Delivery Market Analysis 2022: Industry Overview, Growth, Trends, Opportunities and Forecast Till 2027

 According to IMARC Group’s latest report, titled “RNA Interference (RNAi) Drug Delivery Market: Global Industry Trends, Share, Size, Growth, Opportunity and Forecast 2022-2027”, the global market reached a value of US$ 54.74 Billion in 2021. RNA interference (RNAi) drug delivery is a fundamental cellular mechanism that studies gene functions in…

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Escherichia coli K-12 substr. MG1655 ackA-pta

BioCyc ID:TU0-8301 Local Context (not to scale — see Genome Browser for correct scale) Transcription Unit Notes: Click on a binding site (if any) to navigate to the page for its transcription factor. Click on a gene to navigate to its gene page. Key to regulator colors: Green: activator; Magenta:…

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Understanding the relationship between large-scale chromatin structure and gene expression

Abstract Eukaryotic genomes are packaged into a complex DNA, RNA, and protein rich chromatin fibre, creating an interdependent functional relationship between the structure of the chromatin and the activity of the genetic features stored within. The composition of active genomic regions is weighted towards transcription factors, co-activating proteins, and active …

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GSEA RNASeq

GSEA RNASeq 0 Hi friends For gene set enrichment analysis (GSEA), the software from broad institute does not accept ensemble IDs, I want to do the analysis using entrez ID or hugo ID but about 2000 genes don’t have hugo ID or entrez ID. What should I do? gene enrichment…

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RNA interference triggers that target SARS-CoV-2 genome

Coronavirus disease 2019 (COVID-19) vaccines have played a critical role in reducing transmission of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) transmission. However, with emerging reports of waning vaccine efficacy, there remains an urgent need to develop prophylactic measures against COVID-19. In a recent study published on the bioRxiv*…

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Construction of a circRNA-Mediated ceRNA Network Reveals Novel Biomarkers for Aortic Dissection

Background: Aortic dissection (AD) is a rare and lethal disorder with its genetic basis remains largely unknown. Many studies have confirmed that circRNAs play important roles in various physiological and pathological processes. However, the roles of circRNAs in AD are still unclear and need further investigation. The present study aimed…

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ASM1860456v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI RefSeq Annotation Date::06/02/2021 10:26:31 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method::Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision::5.2 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::3,541 CDSs (total)::3,440 Genes (coding)::3,416 CDSs (with protein)::3,416 Genes (RNA)::101 rRNAs::9, 9, 9 (5S, 16S, 23S) complete rRNAs::9, 9,…

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Whole-genome analysis of coxsackievirus B3 reflects its genetic diversity in China and worldwide | Virology Journal

Song Y, Zhang Y, Han Z, Xu W, Xiao J, Wang X, et al. Genetic recombination in fast-spreading coxsackievirus A6 variants: a potential role in evolution and pathogenicity. Virus Evol. 2020;6:2. doi.org/10.1093/ve/veaa048. Article  Google Scholar  Chen J, Han Z, Wu H, Xu W, Yu D, Zhang Y. A large-scale outbreak…

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Why so much variability in RNA-seq technical replicates?

Why so much variability in RNA-seq technical replicates? 1 Hi all, I download an RNA seq data for gene expression analysis from NCBI. When I did counts with bedtools for my gene of interest, the variability was too much. Does that mean this data is unreliable? What could have caused…

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Counts from recount3 for DESeq2 analysis

Hi, As the topic title suggests, I want to use RSE objects from recount3 for differential expression analysis with DESeq2. This is an ongoing project that I took over. Looking at what has already been done, there are two points I wonder about : 1 – I found that the…

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Bioinformatics Scientist III – D3b at Children`s Hospital of Philadelphia

Job details Job type full-time Full job description Location: loc_roberts-roberts ctr pediatric research req id: 134035 shift: days employment status: regular – full time job summary the bioinformatics unit (bixu) within the center for data driven discovery (d3b) at the children’s hospital of philadelphia (chop) is seeking a level iii…

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Know Market Dynamics, Opportunities and Risks 2027

The Oligonucleotide Pool Library Market Size and Forecast to 2027, this report provides an analysis of the impact of the COVID19 epidemic on the key points influencing the growth of the market. In addition, SaaS Online Video Platforms market segments (by major players, types, applications, and major regions) outlook, company…

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8. Metagenomic study_2022 (1).pdf – Week 8 Metagenomic study focusing on antibiotic resistance genes from food Metagenomics? The term “metagenomics” was

1Week 8Metagenomic study focusing on antibiotic resistance genes from foodMetagenomics?The term “metagenomics” was first used byJo Handelsman,Jon Clardy,Robert M. Goodman, Sean F.Brady, and others, and first appeared in publication in 1998. The term metagenome referenced the ideathat a collection of genessequenced from the environment could be analyzed in a way…

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The low successful assignment ratio of FeatureCounts

Hello, I would like to confirm if the low assignment ratio (54%) is normal, and please check the possible reason I found. I used Hisat2 to assign paired-end strand-specific transcriptomic sequences (rRNA removed) to a reference genome. Because I filtered out the unmapped sequences in advance, the overall assignment ratio…

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Transcriptional profiling of macrophages reveals distinct parasite stage-driven signatures during early infection by Leishmania donovani

Infection with L. donovani amastigotes or promastigotes promotes early changes in the mRNA pool of the host cell To compare the early effects of the two life stages L. donovani in the mature mRNA pool of the host cell, total cytosolic mRNA extracts from bone marrow-derived macrophage (BMDM) cultures infected…

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Postdoctoral Fellow for Bioinformatics, Machine Learning and Biomarker Discovery, Tampa, Florida

At Moffitt Cancer Center, we come face-to-face with cancer every day, but we also see courage. And it inspires us to be the safest and best place for cancer care – to bring greater hope to every patient we serve. It’s why we’ve been continually named One of the Top…

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CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology

Bartel, D. P. MicroRNAs: Target recognition and regulatory functions. Cell 136, 215–233 (2009). CAS  Article  Google Scholar  Bassett, A. R. et al. Understanding functional miRNA–target interactions in vivo by site-specific genome engineering. Nat. Commun. 5, 4640 (2014). ADS  CAS  Article  Google Scholar  Moore, M. J. et al. miRNA–target chimeras reveal…

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Delivery systems of plasmid DNA and messenger RNA for advanced therapies

April 15, Kawasaki/Japan Innovation Center of NanoMedicine (Center Director: Prof. Kazunori Kataoka) has announced that Prof. Satoshi Uchida (Principal Research Scientist), who is also Associated Professor of the Kyoto Prefectural University of Medicine, published an article review about non-viral messenger RNA (mRNA) and plasmid DNA (pDNA) delivery by addressing the…

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N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance | Military Medical Research

Bioinformatics analyses revealed the involvement of m6A modification in cervical cancer progression To better understand whether and how m6A regulators contribute to cervical cancer progression, we first identified 9 m6A writers (WTAP, ZC3H13, METTL3, METTL14, METTL16, VIRMA, RBM15B, RBM15, and CBLL1), 15 m6A readers (FMR1, hnRNPA2B1, hnRNPC, YTHDF1/2/3, YTHDC1/2, LRPPRC,…

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Nature Papers on CRISPR-Cas9 Gene Editing, HSC Development

New details about the mechanism by which DNA is unwound so that its target sequence can be read during CRISPR-Cas9 gene editing are reported in this week’s Nature Structural & Molecular Biology. The study provides insights that may help in the development of improved genome editors. During CRISPR-Cas9 editing, Cas9…

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CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles

This article was originally published here Anal Chim Acta. 2022 May 1;1205:339749. doi: 10.1016/j.aca.2022.339749. Epub 2022 Mar 22. ABSTRACT The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design…

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RCSB PDB – 7V94: Cryo-EM structure of the Cas12c2-sgRNA-target DNA ternary complex

RNA-guided CRISPR-Cas nucleases are widely used as versatile genome-engineering tools. Recent studies identified functionally divergent type V Cas12 family enzymes. Among them, Cas12c2 binds a CRISPR RNA (crRNA) and a trans-activating crRNA (tracrRNA) and recognizes double-stranded DNA targets with a short TN PAM … RNA-guided CRISPR-Cas nucleases are widely used…

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