Tag: RNA

Get mRNA sequence from amino acid sequence using BioPython 1 I have a sequence of amino acids like “MVLLV” and I want to know what mRNA corresponds to that, I’m using biopython for this, but I just know the back_transcribe(), to revert the RNA to DNA, how can I do…

Working with UsChallenging. Meaningful. Life-changing. Those aren’t words that are usually associated with a job. But working at Bristol Myers Squibb is anything but usual. Here, uniquely interesting work happens every day, in every department. From optimizing a production line to the latest breakthroughs in cell therapy, this is work…

For this year’s women and girls in science day, UAR chose to highlight the career of a world-renowned scientist and how animal research shaped her path to becoming one of the most respected and revered researchers. Working in fundamental science and microbiology, Emmanuelle Charpentier gradually obtained prizes, eventually receiving one…

Moderate Mapping percentage 1 Hi all, I received my sequenced transcriptome and genomic data from my service provider and started working with it. Both the DNA and RNA data passed quality metrics post trimming. But the mapping percentage comes out to be 90% using bowtie-DNA and 85% using Hisat2-RNA. I…

Contigs number vs NNN gap % in WGS 0 Hi all, I have two fasta files of bacterial whole genome sequence assembly (same genome) generated by spades generated by spades > extra scaffolding step using multi-csar, by comparing it to 5 close genomes. quast statistics as below : Genome 1…

(MENAFN– GlobeNewsWire – Nasdaq) The Global Bioinformatics Market was valued USD 10.23 Billion in 2023 and projected to reach USD 28.6 Billion by 2030, growing at a CAGR of 13.73% during the forecast period of 2023-2030 Richmond, Feb. 09, 2024 (GLOBE NEWSWIRE) — According to a research report ” Bioinformatics…

OSAKA, Japan–(BUSINESS WIRE)– Shionogi & Co., Ltd. (Head Office: Osaka, Japan; Chief Executive Officer: Isao Teshirogi, Ph.D.; hereafter “Shionogi”) announced the first peer-reviewed publication of the Phase 3 portion of its pivotal, double-blind, randomized, placebo-controlled Phase 2/3 study (SCORPIO-SR) in patients with mild to moderate COVID-19 in Japan, South Korea…

Job DescriptionThis is a Pre-Fellowship for early career researchers wanting to establish a career in MND translational research and become MND research leaders of the future. The postholder will have a leading role in an exciting new project funded by Motor Neurone Disease Association and MND Scotland. The project is…

Mohammed VI Polytechnic University is an institution dedicated to research and innovation in Africa and aims to position itself among world-renowned universities in its fields The University is engaged in economic and human development and puts research and innovation at the forefront of African development. A mechanism that enables it…

Shondra Pruett-Miller, a biochemist at St. Jude Children’s Research Hospital, found her calling during her first year of graduate school at the University of Texas, Southwestern. In 2003, during the departmental faculty research talks, she watched with excitement as Matthew Porteus, now a biochemist at Stanford University, presented his research…

In a groundbreaking development, researchers from NYU Grossman School of Medicine have unveiled a novel technique called Long Range Cleavage sequencing (LORAX-seq). This innovative method can pinpoint the start and end of backtracking events in genetic material, offering unprecedented insights into the intricate world of genetics. Unraveling the Mysteries of…

Entry 118725205         ncRNA     T07682                                  Name (RefSeq) U1 spliceosomal RNA   KO K14276   U1 spliceosomal RNA Organism pkl  Pipistrellus kuhlii (Kuhl’s pipistrelle) Pathway pkl03040   Spliceosome Brite KEGG Orthology (KO) [BR:pkl00001] 09120 Genetic Information Processing  09121 Transcription   03040 Spliceosome    118725205 09180 Brite Hierarchies  09182 Protein families: genetic information processing   03041 Spliceosome [BR:pkl03041]    118725205  09184 RNA family   03100 Non-coding RNAs [BR:pkl03100]    118725205Spliceosome [BR:pkl03041] Splicing related RNAs  118725205Non-coding RNAs [BR:pkl03100] Small non-coding…

Entry 4332822           CDS       T01015                                  Name (RefSeq) LOW QUALITY PROTEIN: nuclear cap-binding protein subunit 1-like   KO K12882   nuclear cap-binding protein subunit 1 Organism osa  Oryza sativa japonica (Japanese rice) (RefSeq) Pathway osa03013   Nucleocytoplasmic transport osa03015   mRNA surveillance pathway osa03040   Spliceosome Brite KEGG Orthology (KO) [BR:osa00001] 09120 Genetic Information Processing  09121 Transcription   03040 Spliceosome    4332822  09122 Translation   03013 Nucleocytoplasmic transport    4332822   03015 mRNA…

Richmond, Feb. 09, 2024 (GLOBE NEWSWIRE) — According to a research report “Bioinformatics Market”, by tools Type (BLAST, BioPerl, InterMine, Others), Services (Genome Sequencing, RNA sequencing, Metagenomic Analysis, Epigenomic, Others), Application (Drug discovery, Personalized medicine, Preventive medicine, Transcriptions, Metabolomics, Gene therapy, Others) Sectors (Medical Biotechnology, Animal Biotechnology, Plant Biotechnology, Environmental…

The Science Newswise — CRISPR-Cas is a tool that allows scientists to make targeted changes to an organism’s DNA. This tool consists of two parts. The first is a microscopic substance called a nuclease Cas protein (e.g., Cas9) that can cleave DNA. The second is an RNA molecule (also called…

dc.contributor.author Lim, Ryan G dc.contributor.author Al-Dalahmah, Osama dc.contributor.author Wu, Jie dc.contributor.author Gold, Maxwell P dc.contributor.author Reidling, Jack C dc.contributor.author Tang, Guomei dc.contributor.author Adam, Miriam dc.contributor.author Dansu, David K dc.contributor.author Park, Hye-Jin dc.contributor.author Casaccia, Patrizia dc.contributor.author Miramontes, Ricardo dc.contributor.author Reyes-Ortiz, Andrea M dc.contributor.author Lau, Alice dc.contributor.author Hickman, Richard A dc.contributor.author Khan,…

Precision Therapies For Targeted Results Precision therapies have always been the ultimate goal of medicine. Instead of drugs acting on multiple parts of the body, these therapies would only target one organ, one cell, or even one gene. In theory, not only would this be a lot more efficient, but…

Cho, C. et al. Haploinsufficiency of protamine-1 or-2 causes infertility in mice. Nat. Genet. 28, 82–86 (2001). Article  CAS  PubMed  Google Scholar  Oliva, R. Protamines and male infertility. Hum. Reprod. Update 12, 417–435 (2006). Article  CAS  PubMed  Google Scholar  Balhorn, R. The protamine family of sperm nuclear proteins. Genome Biol….

In an extremely proud moment for Odisha, a team of researchers at the prestigious Institute of Life Sciences (ILS) in Bhubaneswar has made a groundbreaking discovery that suggests the role of direct interaction of circRNAs with mRNAs in gene expression regulation. The findings represent a novel method for illuminating the…

The Biopython logo Biopython is a set of tools for doing all sorts of genomics tasks: reading DNA and RNA, aligning sequences, analyzing similarities between sequences, and more. This is the second part in a series on analyzing coronavirus RNA with Python. Here we’ll install Biopython and use it to…

Mohammed VI Polytechnic University is an institution dedicated to research and innovation in Africa and aims to position itself among world-renowned universities in its fields The University is engaged in economic and human development and puts research and innovation at the forefront of African development. A mechanism that enables it…

Metagenomi, a preclinical biotech using metagenomics-derived genome editing, raised $94 million by offering 6.3 million shares at $15, the low end of the range of $15 to $17. Metagenomi is a precision genetic medicines company developing curative therapeutics using its proprietary, comprehensive metagenomics-derived genome editing toolbox. Its toolbox includes…

At the end of 2023, the FDA approved Casgevy for sickle cell disease, its first approval for a therapeutic that used the genome editing tool clustered regulatory interspaced palindromic repeats (CRISPR) to specifically inactivate a human gene as a treatment for a genetic disease. While CRISPR-based gene therapies could potentially…

The UK is home to several biotech hubs, including the famous ‘golden triangle’, made up of Oxford, Cambridge, and London. In fact, after a $700 million surge in funding in the third quarter of 2023, the UK biotech scene is now one of the most vibrant in Europe, laying the…

Gene editing technologies, particularly those based on Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR), have revolutionized our ability to accurately and swiftly detect pathogens. However, even these advanced methods have their limitations. This article explores these limitations and highlights recent advancements that are paving the way for faster, more accurate…

Single nucleus RNA-seq reveals cell type heterogeneity in human SNpc We sampled SNpc from post-mortem human brains of 15 sporadic Parkinson’s disease (PD) patients and 14 Control individuals (see Supplementary Table 1 for full pathology reports). Using a 10X Genomics Chromium platform, we performed single nucleus RNA-seq (snRNA-seq) on more than…

About 1.3 billion adults worldwide have clinically high blood pressure, many of whom may , [+] Unaware that they are living in this situation. getty Approximately 1.3 billion adults worldwide have clinically high blood pressure, many of whom may be unaware that they are living with the condition. As a…

Identification of putative anti-crispr proteins using structural features To identify Acrs in phage genomes, we began by retrieving ~66.5 million proteins from Integrated Microbial Genomes Virus database (IMG/VR)37. We excluded large proteins because over 90% of known Acrs contain less than 200 amino acids38 (Fig. 1A, Supplementary Data 1). To reduce computational…

What are the applications of gene transfer in the treatment of various diseases?5 answersGene transfer has various applications in the treatment of different diseases. It can be used for gene therapy, where new genes are inserted into cancer cells to slow down their growth or induce cell death. Gene transfer…

Running Deseq2 with all samples vs samples for each comparison separately 1 @52b8b937 Last seen 5 hours ago Portugal Hello, I am currently using Deseq2 to perform differential analysis on my data. I have feature count data for 24 samples and 4 comparisons to do ( for each comparison I…

Bacteria Strain Cori.ST1911 was isolated from fresh stool of 20-week-old C57/BL6J mice fed a HFD at the Animal Center of the West China Hospital of Sichuan University. Briefly, faecal particles were ground with a glass grinding rod and suspended in sterile phosphate-buffered saline (PBS). After gradient dilution, the suspension was…

Ch 13 Learning Objectives Section 13.1 1. Differentiate between the structures of DNA & RNA (Table 13.1, Fig 13.1) 2. Know the locations and functions of rRNA, mRNA, and tRNA (Table 13.2)  For now, you only need to know rRNA, mRNA, tRNA. We will learn the others in future…

Deseq2 output and robust PCA (PcaGrid and PcaHubert) 0 Hello there, I followed the deseq2 file to get the list of differentially expressed genes of a dataset I am working on. The Pca graph I got was not enough to show which one of the replicate is an outlier. A…

Capturing proteins associated with SARS-CoV-2 virions To identify host cell proteins potentially present in extracellular SARS-CoV-2 virions, viral particles were produced from two lung epithelial cell models, A549 cells overexpressing ACE2 receptor (A549-ACE2) and Calu-3 cells that naturally express ACE2 receptor and TMPRSS2. Following infection with the D614G SARS-CoV-2 strain32,…

Background: IgE has been known for mediating endothelial cell dysfunction and mast cell (MC) activation to fuel asthma-aggravated high-fat diet-induced atherosclerosis. However, it remains unclear for the mechanism of asthma-mediated atherosclerosis, especially the potential involvement of IgE in the exacerbation of asthma-mediated atherosclerosis with a standard laboratory diet, and the…

We are seeking a talented, highly motivated Senior Bioinformatics Research Scientist to lead our bioinformatics pipeline and develop innovative solutions for analyzing and visualizing omics data related to innate immune signaling projects. The candidate will work both collaboratively and independently on projects within the Kanneganti lab in the Department of…

Catalog number PVTB80045-3a Price 356 EUR Size 2 ug Kit Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most…

Problem with DRAGEN RNAseq hashtable directory 1 Dear all, Recently I wrote a code to work with DRAGEN and RNAseq pipeline. I use this command: /opt/edico/bin/dragen -f -l \ -r refdir \ -1 ${forward} \ -2 ${reverse} \ -a ${gtf} \ –output-dir output/${sample} \ –output-file-prefix ${sample} \ –RGID ${sample}_group_id \…

Burgess HM, Depledge DP, Thompson L, Srinivas KP, Grande RC, Vink EI, Abebe JS, Blackaby WP, Hendrick A, Albertella MR, Kouzarides T, Stapleford KA, Wilson AC, Mohr I. Targeting the m6A RNA modification pathway blocks SARS-CoV-2 and HCoV-OC43 replication. Genes Dev. 2021;35:1005–19. Article  CAS  PubMed  PubMed Central  Google Scholar  Chambers…

Gladyshev, V. N. et al. Molecular damage in aging. Nat. Aging 1, 1096–1106 (2021). Article  PubMed  PubMed Central  Google Scholar  Sziráki, A., Tyshkovskiy, A. & Gladyshev, V. N. Global remodeling of the mouse DNA methylome during aging and in response to calorie restriction. Aging Cell 17, e12738 (2018). Article  PubMed …

[](https://travis-ci.org/QuackenbushLab/yarn) # YARN: Robust Multi-Tissue RNA-Seq Preprocessing and Normalization The goal of yarn is to expedite large RNA-seq analyses using a combination of previously developed tools. Yarn is meant to make it easier for the user to perform accurate comparison of conditions by leveraging many Bioconductor tools and…

Forgive me if this post is messy, I’m new to this! I’m analyzing RNA Seq data and found that one of my samples is an outlier (sample AV17). I’m trying to exclude it from my analysis, but whenever I do, using this code: dds = subset(countData, select = -c(AV17) ),…

A few hours before the CASP14 (14th Critical Assessment of Structure Prediction) meeting, the latest biannual structure prediction experiment where participants build models of proteins given their amino acid sequences, this image went viral on twitter. Ranking of participants in CASP14, as per the sum of the Z-scores of their…

Natural killer (NK) cells are white blood cells that are a part of the body’s defense mechanism. These cells are on the lookout for any kind of disruption in the immune system, and can kill diseased cells like tumor cells. NK cells are being used in clinical applications to treat…

Troubleshooting RNA-seq data with DNA contamination 0 Hi everyone, If there has been DNA contamination in the RNA samples and RNA-seq was conducted. After RNA-seq, I tried using SeqMonk and I estimated contamination levels, which were highly variable between samples, ranging from 1.7% to 10.5%. My initial step was to…

Florida is currently home to the nation’s second-largest medical device manufacturing industry, second-largest pharmaceuticals manufacturing industry, and the fourth-largest biotech R&D industry, making it a large life sciences hub within the U.S. So, in this article, with more than 2,300 establishments operating within the Florida life sciences industry as a…

When CASGEVY, the first CRISPR-based therapy, was approved by the FDA in December, it gave CRISPR technology the validation it needed. Any promise it had held before that moment was simply investigational. In this case, the approval of CASGEVY was granted for the treatment of two blood disorders – sickle…

Vincerx Pharma, Inc. Investigators from the National Institutes of Health (NIH) report 2 partial responses (PR) in 3 peripheral T-cell lymphoma (PTCL) patients and 1 PR in 2 double-hit diffuse large b-cell lymphoma (DH-DLBCL) patients in ongoing dose-escalation trial of enitociclib in combination with venetoclax and prednisone Vincerx remains on…

Less than 15% of the global population lives in Europe or North America. Yet more than 70% of published human microbiome data — on the collections of bacteria, fungi and viruses that live on and in our bodies — comes from European and North American populations1. Around 85% of the…

This protocol enables the impact of prophages on their hosts to be revealed. Bacterial cultures are synchronized using conditions that best support the lysogenic state, limiting spontaneous induction. RT-qPCR unequivocally distinguishes prophage-restricted genes and those uncoupled from phage control from those that are expressed during the lytic replication cycle. Prophages…

CDC. (ed CDC) (U.S. Department of Health and Human Services, CDC; 2019, Atlanta, GA, 2019). Krismer, B., Weidenmaier, C., Zipperer, A. & Peschel, A. The commensal lifestyle of Staphylococcus aureus and its interactions with the nasal microbiota. Nat. Rev. Microbiol. 15, 675–687 (2017). Article  CAS  PubMed  Google Scholar  Somerville, G….

A, 3-D models of human ribosomal subunits based on PDB entries 3J3D and 3J3A (for SSU r-proteins), and on 3J3F and 3J3B (for LSU r-proteins) highlighting the position of individual r-proteins on mature subunits. On small subunits, the 18S rRNA is shown in grey; on large subunits, the 5S, 5.8S…

Ethics and animal welfare statement All infectious work with RESTV, including sample inactivation, was performed in the Containment Level 4 laboratory (CL4) in accordance with the policies and protocols outlined by the Canadian Science Centre for Human and Animal Health Institutional Biosafety Committee. All animal work was performed in strict…

Finding EntreZ IDs for refseq IDs 1 Hi all, I have a list of bacterial RefSeq IDs corresponding to protein sequences (e.g., WP_007430823.1, WP_019686959.1, etc.). I need to retrieve the corresponding EntreZ IDs for these RefSeq IDs, in order to cotinue the RNA-seq downstream analysis (GO enrichment analysis ). Here’s…

It said staff at the Taixing People’s Hospital submitted the genetic sequence to the GenBank sequence database, an open access collection maintained by the National Centre for Biotechnology Information in the United States. In December, the US centre said the nucleotide sequence present in the sample had not been detected…

The dream of a cancer vaccine — capable of stimulating the immune system to fight tumors — is on the horizon of the scientific community. It probably has never been as close as it is now. After years of few successes in this field, the consolidation of other immunotherapies and…

ZUG, Switzerland and BOSTON, Jan. 08, 2024 (GLOBE NEWSWIRE) — CRISPR Therapeutics (Nasdaq: CRSP), a biopharmaceutical company focused on creating transformative gene-based medicines for serious diseases, today highlighted its strategic priorities and 2024 outlook as the Company enters its next phase of growth. “We had a landmark year in 2023, marked…

RNA-seq deconvolution algorithm that takes into account weight of marker gene 1 Hi all, Some deconvolution algorithms on RNA-seq data requires a list of marker genes as reference for different cell types. However, does anyone know whether any of these have the option to specify the “importance” or weight of…

Quantile normalisation on RNAseq with substantial differences on sample size 0 I tried vst and rlog from DESEq2 for my RNA seq data. But i suspect the largest group (condition 1 with 60 samples) has affected the variance from other groups (condition2 with 20 samples, condition 3 and 4 with…

CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared…

Reference genome, BWA and right algorithm 1 Hello I’m using BWA to create the index for aligning some rna-seq fastq. First thing I did was download hg38.fa.align.gz from UCSC Then I: gzip -d hg38.fa.align.gz sudo apt-get install bwa Here comes the problem. BWA instructions reccomend bwtsw algorithm, but when I…

What are the limitations of using 16S sequencing for taxonomic identification?4 answers16S sequencing for taxonomic identification has several limitations. The most commonly used bioinformatics tools, such as Qiime 2 and Mothur, may underestimate the accuracy of species-level taxonomic assignments. Additionally, current analytical approaches lack the ability to formalize syntheses of…

Guangzhou Geneseed Biotech. Co.,Ltd. founded in June 2010,is a high-tech enterprise focusing on circRNA technology research and industrialization development.Since 2014, Geneseed has been focusing on the circRNA segment, and continuously cultivating for nearly a decade, with a profound accumulation of original technologies. Our team has professionals in microbiology, molecular biology, bioinformatics,…

Cancer is one of the leading causes of disease-associated mortality, with a rising incidence worldwide [1]. Although many therapeutic methods have been used in cancer treatment, such as chemotherapy, radiotherapy, surgery, and targeted therapy, the overall therapeutic outcome remains unsatisfactory. Therefore, developing new therapeutic means is urgently needed. With advances…

Objectives: Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis worldwide. HEV RNA detection is the gold standard for HEV infection diagnosis and PCR methods are commonly used but are usually time-consuming and expensive, resulting in low detection efficiency and coverage, especially in low-income areas. Here, we…

Credit: Linda Bartlett/National Cancer Institute, via Wikimedia Innovent Biologics and SanegeneBio have entered into a collaboration and co-development agreement for SGB-3908, an siRNA drug candidate targeting angiotensinogen (AGT) for the treatment of hypertension. Under the terms of the deal, Innovent will gain an exclusive option to license future development, manufacturing,…

Staphylococcus aureus sepsis and mitochondrial accrual of the 8-oxoguanine DNA glycosylase DNA repair enzyme in mice. Publication ,  Journal Article Bartz, RR; Suliman, HB; Fu, P; Welty-Wolf, K; Carraway, MS; MacGarvey, NC; Withers, CM; Sweeney, TE; Piantadosi, CA Published in: Am J Respir Crit Care Med RATIONALE: Damage to mitochondrial DNA…

Abstract RNA interference is a powerful tool for controlling gene expression in mammalian systems; as such, it has become an effective alternative to conventional knockout approaches. RNAi has proven to be an efficient method to inhibit tumor suppressor gene function and yield insight into the important players in cancer biology….

Wang MY, Jia JC, Yang CD, Hu QY. Pathogenesis, disease course, and prognosis of adult-onset Still’s disease: an update and review. Chin Med J (Engl). 2019;132(23):2856–64. Article  CAS  PubMed  Google Scholar  Feist E, Mitrovic S, Fautrel B. Mechanisms, biomarkers and targets for adult-onset Still’s disease. Nat Rev Rheumatol. 2018;14(10):603–18. Article …

Brent, G. A. Mechanisms of thyroid hormone action. J. Clin. Invest. 9, 3035–3043 (2012). Article  Google Scholar  Iwen, K. A., Oelkrug, R. & Brabant, G. Effects of thyroid hormones on thermogenesis and energy partitioning. J. Mol. Endocrinol. 60, R157–R170 (2018). Article  PubMed  CAS  Google Scholar  Biondi, B. & Wartofsky, L….

Team Leader Bioinformatics – Ryvu Therapeutics, Krakow Team Leader Bioinformatics Ryvu Therapeutics Krakow, Poland As Bioinformatics Team Leader you will be responsible for development of this area within Data Science department which includes managing and growing the team, setting and executing short and long term plans, propagating collaboration with other…

Reagents and antibodies The details of the drugs and related reagents used in this study are presented in Additional file 1: Table S1. Animals A total of 48 male C57BL/6 mice were purchased from Byrness Weil Biotech Ltd. (License number: SCXK (Xiang) 2019-0004). The mice were of SPF-grade and had…

Anavex Life Sciences Corp. (“Anavex” or the “Company”) (Nasdaq: AVXL), a clinical-stage biopharmaceutical company developing differentiated therapeutics for the treatment of neurodegenerative and neurodevelopmental disorders including Alzheimer’s disease, Parkinson’s disease, Rett syndrome and other central nervous system (CNS) diseases, today announced the first entire clinical gene pathway data from the…

MOST people will primarily associate protein with its role as a major food group. But at a cellular level, there are millions of possible proteins: they are large, complex molecules that play crucial roles in biochemical processes. A single protein molecule is made up of amino acids linked together in…

Hello, Galaxy Community! Firstly, the Galaxy team would like to extend our warmest wishes to you and all your loved ones this holiday season! With the New Year rapidly approaching, we wanted to thank you all for being a part of the Galaxy Community in 2023; we couldn’t do it…

Key Points Question  What is the association of PIK3CA mutations, response to therapy, and outcome by hormone receptor (HR) status and intrinsic subtype among patients with ERBB2/HER2-positive early breast cancer (EBC) treated in a clinical trial? Findings  In this cohort study of 184 patients enrolled in the phase 3 trial…

doi: 10.1093/nar/gkad1202. Online ahead of print. Nidhi Kanwal  1 , Nicolai Krogh  2 , Indira Memet  1 , Nicolas Lemus-Diaz  1 , Chairini C Thomé  1 , Luisa M Welp  3   4 , Athanasia Mizi  5 , Philipp Hackert  1 , Argyris Papantonis  5 , Henning Urlaub  3   4   6   7 , Henrik Nielsen  2 , Katherine…

MAVS maintains mitochondrial homeostasis and regulates cellular senescence through stabilizing OPA1. A. Depletion of MAVS accelerates cellular senescence of human mesenchymal stem cells. B. MAVS deficiency compromises mitochondrial dynamics. C. A model illustrating the MAVS-OPA1 axis regulates mitochondrial homeostasis and human stem cell senescence. Credit: Research A recent study led…

Job Description 6000! This is the number of associates in the Novartis Institutes for BioMedical Research (NIBR). This division is the innovation engine of Novartis, focusing on new technologies that have the potential to help produce therapeutic breakthroughs for patients. The Biologics Center (NBC) is accommodated within the Novartis Institutes…

Human tissue samples The preoperative diagnosis of CS mainly relies on stool microscopy, enzyme-linked immunosorbent assay, or endoscopy. Fresh liver and intrahepatic bile duct specimens were collected from ICC patients with (CS-ICC group) and without (NC-ICC group) CS infection during partial hepatectomy at the First Hospital of Jilin University. Tumor tissues…

Since the idea of genetic regulation was first postulated back in the 1950s, our scientific understanding of the transcriptome has deepened greatly.1 Looking into the transcriptome of cells and tissues has helped scientists to understand the biological processes that drive both health and disease; however, the complex and occasionally mysterious…

Li Q, Song ZL, Zhang YX, Zhu LN, Yang Q, Liu XF, Sun XF, Chen XX, Kong RM, Fan GC, Luo XL (2023) Synergistic Incorporation of two ssDNA activators enhances the trans-cleavage of CRISPR/Cas12a. Anal Chem 95:8879–8888 Article  CAS  PubMed  Google Scholar  Ma JY, Wang SY, Du YC, Wang DX,…

Localization of developmentally regulated Ψ sites on the trypanosomatid ribosome. Differentially regulated Ψ sites in T. brucei total rRNA are shown on the 3D structure of the ribosome. Credit: Nature Communications (2023). DOI: 10.1038/s41467-023-43263-6 Trypanosome is a single-celled parasite that takes an extensive human and economic toll due to its…

Animals C57BL/6-Tg (Pf4-icre) Q3Rsko/J (Pf4-Cre) mice were purchased from the Jackson Laboratory (Bar Harbor, ME, USA). B6;129-Tmem173tm1(flox)Smoc (Stingfl) mice were purchased from Shanghai Model Organisms Center, Inc. (Shanghai, China). C57BL/6J-Ifnar1em1(flox)Cya (Ifnar1fl) mice were purchased from Cyagen Biosciences (Guangzhou, China). Stingcko and Ifnar1cko mice were generated by crossing Pf4-Cre mice with…

DNA sequencing I. Goal II. Methodology A. Sanger DNA sequencing ● Up to 900 base pairs ● Primer extension with labeled ddNTPs (radioactive or fluorescent) ● Prior knowledge on target needed ● Difficult to detect variation in mixtures ● High accuracy + still in use for genotyping When it is…

Forum:LncRNA pipeline 0 HI ALL, I AM WORKING ON FINDING LNCRNA, I WOULD LIKE TO CHECK IF MY PIPELINE IS RIGHT FIRST I MAP USING STAR AND THEN SORT IT AND THEN GO FOR HTSEQ -GET THE COUNT DATA AND DO DESEQ. FOR LNCRNA EXTRACTION FROM RNA SEQ, I AM…

Introduction Brucellosis is a zoonotic disease caused by Brucella spp., a gram-negative facultative intracellular coccobacillus.1 These microorganisms can infect livestock, wildlife, and humans, causing significant public concern and substantial agricultural economic loss. Currently, at least six novel Brucella species have been identified: Brucella pinnipedialis, Brucella ceti,2 Brucella papionis,3 Brucella microti,4…

– The announcement follows the company’s groundbreaking clinical data presented at the American College of Rheumatology annual meeting HAYWARD, Calif., Dec. 19, 2023 /PRNewswire/ — Aqtual, a precision medicine company with a novel cfDNA platform to aid in treatment decisions for chronic diseases and cancers through a simple blood test,…

Is there a list of mouse housekeeping genes available to download? 3 Hello everyone, I have searched though BioStars and although many threads ask about list of housekeeping genes, no answer can be found. So I’m taking the liberty of asking again: in there a comprehensive list of housekeeping genes…

The Biostar Herald publishes user submitted links of bioinformatics relevance. It aims to provide a summary of interesting and relevant information you may have missed. You too can submit links here. This edition of the Herald was brought to you by contribution from Mensur Dlakic, Istvan Albert, and was edited…

Introduction The incidence and mortality rates of AF are continuously increasing, leading to serious complications such as heart failure and stroke (Fig. 1) (Sagris et al., 2021). The occurrence and development of AF involve different mechanisms and interactions (Kornej et al., 2020). AF often progresses from paroxysmal to persistent (Nattel…

Analysis of the multisegmented nature of BcssDV1 genome B. cinerea field isolates were previously obtained from infected grapes of vineyards of Italy and Spain and their mycovirome was determined [8]. A new ssDNA virus (BcssDV1, Genbank accession no. MN625247) was discovered and characterized. The sequence previously characterized of BcssDV1 (from…

Azenta Life Sciences will set up a new European life sciences hub in Oxford, UK, scheduled to open in March 2024.  The site will feature a new genomics lab called Genewiz Multiomics and Synthesis Solutions. The site will make use of the experienced team from the former Oxford Genomics Centre (OGC) and aim to provide fast…

Faculty and trainees from the University of Chicago Medicine Comprehensive Cancer Center (UCCCC) joined the world’s largest hematology community to discuss the latest updates in blood cancers at the 65th American Society of Hematology (ASH) Annual Meeting and Exposition held December 9-12, 2023 in San Diego and online. At the meeting,…

Roberto Bahena-Ceron1, Chloe Teixeira2, Jose R Jaramillo Ponce1, Philippe Wolff1, Florence Couzon2, Pauline François3, Bruno Klaholz4, François Vandenesch5, Pascale Romby1, Karen Moreau2 and Stefano Marzi1,6 1 Université de Strasbourg, CNRS, Architecture et Réactivité de l’ARN, UPR9002, F-67000 Strasbourg, France; 2 CIRI, Centre International de Recherche en Infectiologie, Université de Lyon,…

Genome assembly and annotation The DNA sequencing data (1119.58 Gb, average 110× coverage) of the A. konjac sample were obtained using the Illumina Hiseq 2500 sequencer. A summary of the sequence data used for the assembly is presented in Table S1. The estimated genome size is 4,512,012,462 bp using 19-mer frequency distribution based…

Rahul Kakkar, MD, Tome Biosciences president and CEO While the CRISPR community celebrates the recent first FDA approval of a CRISPR-Cas9 therapy (Casgevy), new entities are making strides in their efforts to write perhaps the definitive new chapter in programmable genome editing. Last week, Tome Biosciences—a developer of genome editing…

CRISPR/Cas9-induced homozygous knockout of nexn causes progressive cardiac dysfunction without affecting skeletal muscle function in zebrafish Several studies in different animal models and cardiomyopathy patients have shown that loss of NEXN is leading to DCM which is characterized by an impaired contractility of the heart6,10,12. Homozygous loss of NEXN mostly…

Details Posted: 19-Dec-23 Location: Chicago, Illinois Type: Full-time Salary: Open Categories: Research – Laboratory/Non-Laboratory Staff/Administrative Location: Chicago, IL Job Description: Development of an integrated data warehouse for symbiote including clinical, microbiome sequence, and metabolomic data.  Management of DFI Biobank, MMF, and HMMF data within the Symbiote platform. Functional analysis of…

join2-wiki.gsi.de/foswiki/pub/Main/Artwork/join2_logo100x88.png Probing the conformational changes of in vivo overexpressed cell cycle regulator 6S ncRNA Makraki, E. ; Miliara, S. ; Pagkalos, M. ; Kokkinidis, M. ; Mylonas, E. (Corresponding author)Extern*EMBL* ; Fadouloglou, V. E. (Corresponding author)Extern*EMBL* 2023Frontiers Lausanne This record in other databases:   Please use a persistent id in citations: doi:10.3389/fmolb.2023.1219668  doi:10.3204/PUBDB-2023-07967 Abstract: The…