Tag: RNA

Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency

AuNP production Fusarium oxysporum was used for the extracellular production of AuNP. The fungus was acquired from the Culture Collection of Fungi of the Charles University (CCF 3732, Czech Republic). After cultivation in Sabouraud Dextrose Broth (SDB, Sigma Aldrich, Prague, Czech Republic) at 30 °C and 150 rpm for 4 days, the supernatant…

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TET2 inhibits the proliferation and metastasis of lung adenocarcinoma cells via activation of the cGAS-STING signalling pathway

Chen W, Zheng R, Baade PD, Zhang S, Zeng H, Bray F, Jemal A, Yu XQ, He J. Cancer statistics in China, 2015. CA Cancer J Clin. 2016;66(2):115–32. Article  PubMed  Google Scholar  Siegel RL, Miller KD, Jemal A. Cancer statistics, 2020. Cancer J Clin. 2020;70(1):7–30. Article  Google Scholar  Thai AA,…

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The Biostar Herald for Wednesday, September 06, 2023

The Biostar Herald publishes user submitted links of bioinformatics relevance. It aims to provide a summary of interesting and relevant information you may have missed. You too can submit links here. This edition of the Herald was brought to you by contribution from Istvan Albert, and was edited by Istvan…

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Research Fellow / Senior Research Fellow in Bioinformatics job with UNIVERSITY OF SOUTHAMPTON

Enterprise Units Location:  Southampton General Hospital Salary:   £34,980 to £56,021 Per annum Full Time Fixed Term (12 months) Closing Date:  Tuesday 03 October 2023 Interview Date:   To be confirmed Reference:  2428423CM The newly established Faculty of Medicine Bioinformatics Research Facility (Bio-R) serves as a centralised resource for providing…

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Insert size is so small, good or bad?

Insert size is so small, good or bad? 1 If you have the average insert size is ~158 estimated using BBMap . While the read length from the fastq file is 150. so we paired reads length (2*150) This means the paired end reads overlap by too much. Is this…

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First-in-Class Targeted MicroRNA Therapy Blocks Tumor Growth in Mice

Researchers at Purdue University have developed a new type of cancer therapy that uses a modified microRNA, miR-34a, to block cancer cell division. In preclinical tests in mice, treatment using the fully modified, tumor-targeting miR-34a halted tumor growth for the 21-day evaluation period, and resulted in complete cures in some…

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Study identifies potential target for reversing drug resistance in ovarian cancer

For the 314,000 people diagnosed with ovarian cancer each year, hope often comes in the form of platinum-based drugs such as cisplatin. Cisplatin causes the death of quick-dividing tumour cells, so it is a potent first-line defence in the treatment of the often fatal disease. However, over half of ovarian…

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Gene Editing Tools Based on CRISPR/Cas System and Their Improvement Strategies

     Gene Editing Tools Based on CRISPR/Cas System and Their Improvement Strategies XIN Yuan1,TIAN Kai-ren3,QIAO Jian-jun1,2,3,CAIYIN Qing-ge-le1,2,**() 1 School of…

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Celyad Oncology Reports First Half 2023 Financial Results and Recent Business Highlights

Celyad Oncology SA Georges Rawadi was appointed Chief Executive Officer as from April 27, 2023 Celyad Oncology has received approximately EUR 9.8m in private placement commitments from historical shareholders Encouraging progress in multiplex shRNA platform development, which allows now targeting of up to four genes simultaneously, were presented at international…

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Precise RNA Editing: Cascade Self-Uncloaking Dual-Prodrug Nanoassemblies Based on CRISPR/Cas13a for Pleiotropic Immunotherapy of PD-L1-Resistant Colorectal Cancer,Advanced Functional Materials

CRISPR/Cas13a is a powerful genome editing system for RNA knockdown that holds enormous potential for cancer treatment by targeting currently undruggable oncogenes or immune checkpoints. However, the precise intratumoral activation of CRISPR/Cas13a to maximize the therapeutic efficiency while guaranteeing biosafety remains a daunting challenge. Here, a cascade self-uncloaking nanoassembly (SRC)…

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Characterisation and comparative genomics of three new Varanus-associated Borrelia spp. from Indonesia and Australia | Parasites & Vectors

The following results clearly demonstrate that the Borrelia spp. identified in ticks collected from V. salvator, V. varius, and V. giganteus are distinct species. Therefore, for clarity, we herein refer to these Borrelia spp. by their proposed names: Borrelia salvatorii (from V. salvator), ‘Ca. Borrelia undatumii’ (from V. varius), and…

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Biology 1040 Chapter 15 – Be able to correctly label in-groups and branch-points.(s17, s19)

– Be able to correctly label in-groups and branch-points.(s17, s19) outgroup: frog because all examples have 4 limbs Ingroup: iguana, duck-billed platypus, kangaroo, and beaver Branch point: green; derived character: gestation – Define, describe, and evaluate how mtDNA and rRNA are used to infer relatedness. Know-apply when to use each…

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Clinical Oncology Next Generation Sequencing (NGS) Market

Clinical Oncology Next Generation Sequencing Market The Global Clinical Oncology Next Generation Sequencing Market Size was valued at USD 339.10 million in 2022 and is predicted to reach USD 1.18 billion by 2030, increasing at a CAGR of +14% from 2023 to 2030. Next-generation sequencing is a technique that helps…

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Director Of Bioinformatics job in India

India Qrata Full time About Qrata: We match top talent with highly competitive career opportunities with leading start-ups in India and globally and address hiring challenges, recruitment brand marketing, with innovative and collaborative strategic consult.Role : Director Bioinformatics Location : Bangalore or Delhi…

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[Solved] What are the 10 functions of siRNA – Biology 1 (BIO1)

Small interfering RNAs (siRNAs) are a class of double-stranded RNA molecules, 20-25 base pairs in length. They play a significant role in the RNA interference (RNAi) pathway, where they interfere with the expression of specific genes. Here are the 10 functions of siRNA: Gene Silencing: siRNAs are involved in the…

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Over 40 Clinical Trials for mRNA Cancer Vaccines, Phase 3 Trials Prominent; No Commercial Approvals as of August 2023

Company Logo Dublin, Sept. 05, 2023 (GLOBE NEWSWIRE) — The “Global mRNA Cancer Vaccines Market Opportunity & Clinical Pipeline Insight 2023” drug pipelines has been added to ResearchAndMarkets.com‘s offering. The global mRNA cancer vaccines market is undergoing a paradigm shift, propelled by intense research, robust investment, and strategic alliances. This…

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Inter-bacterial mutualism promoted by public goods in a system characterized by deterministic temperature variation

Location and sample collection Samples were taken from 10 composting piles (between May and August 2020) at a food waste (FW) composting facility (Zhejiang, China; 30°52′51.50″N; 119°46′12.87″E). A consistent pre-treatment method was used for each pile. Specifically, about 8 tons of FW was collected every 3 days and subsequently crushed into…

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Research Fellow / Senior Research Fellow in Bioinformatics at University of Southampton

Full Time Fixed Term (12 months) The newly established Faculty of Medicine Bioinformatics Research Facility (Bio-R) serves as a centralised resource for providing expert & timely bioinformatics consulting and data analysis solutions. The BIO-R’s mission is to build and maintain an infrastructure that provides the application of robust bioinformatics to enable…

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Pig H3K4me3, H3K27ac and gene expression profiles reveal reproductive tissue-specific activity of transposable elements

Tao Jiang, Zhimin Zhou, Ziqi Ling, Qing Zhang, Zhongzi Wu, Jiawen Yang, Siyu Yang, Bin Yang, Lusheng Huang. Pig H3K4me3, H3K27ac and gene expression profiles reveal reproductive tissue-specific activity of transposable elements. Zoological Research. doi: 10.24272/j.issn.2095-8137.2023.060 Citation: Tao Jiang, Zhimin Zhou, Ziqi Ling, Qing Zhang,…

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Senior Research Scientist, Bioinformatics job with GenScript

Job Type Full-time We are seeking a motivated and experienced bioinformatics developer to build an AI driven platform focused on analysis of synthetic DNA and mRNA. This will require collecting and analyzing very large data sets built from a variety of sources including sequencing, RNA folding and structure computation and…

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GMP Plasmid DNA Manufacturing Market Size, Share and Scope Analysis to 2031

InsightAce Analytic Pvt. Ltd. has announced the publication of a market research report titled Global GMP Plasmid DNA Manufacturing Market By Product(Viral Vectors(Retroviral, Adenoviral, Lentiviral, Adeno-Associated, Others), Plasmid DNA, Non-viral, Lipid/polymer, Electroporation, Nanoparticles, Others), Types (Pre-Clinical Therapeutics, Clinical Therapeutics, Marketed Therapeutics), Application(Gene Therapy, DNA Vaccines, Immunotherapy)) Market Outlook and Industry…

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Therapeutic Target MicroRNA Identification Based on Circular RNA Expression Signature After Intracerebral Hemorrhage

An SJ, Kim TJ, Yoon BW (2017) Epidemiology, risk factors, and clinical features of intracerebral hemorrhage: an update. J Stroke 19:3–10 Article  PubMed  PubMed Central  Google Scholar  Cordonnier C, Demchuk A, Ziai W, Anderson CS (2018) Intracerebral haemorrhage: current approaches to acute management. Lancet 392:1257–1268 Article  PubMed  Google Scholar  Kim…

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Gut microbes play a starring role in insulin resistance, opening doors for new diabetes treatments

In a recent study published in the journal Nature, researchers utilize a comprehensive multi-omics strategy in humans to assess the association between gut microbial carbohydrate metabolism and insulin resistance (IR). Furthermore, this study discovered links between fecal metabolites and metabolic syndrome (MetS), an IR-related illness. Study: Gut microbial carbohydrate metabolism contributes to…

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The Delights Of The Pfizer/Moderna Catfight

Authored by Robert Malone via The Brownstone Institute, I am a bit embarrassed to admit that I am taking a perverse pleasure in watching this catfight between Pfizer/BioNTech and Moderna play out. Not to gloat, but I find it validating and rather satisfying to watch these companies file lawsuits and…

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prediction dead time within 24 hours with TPM-rna seq data

prediction dead time within 24 hours with TPM-rna seq data 0 Is there a way to know the specific time of death within 24 hours of a patient using TPM rna-seq data in Julia, R, and Python? I’m currently trying to do that analysis with Julia’s CYCLOPS package, but I’m…

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affy expression array data

affy expression array data 0 Hi, everyone I use the oligo package to read cel files, and use the rma function to transformed them into expression matrix, but what I got was a set of Na value. When I used box plot to visualize the data, it showed nothing, but…

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Squid thoughts: Scientists genetically-engineer glass-like cephalopod with brain that glows when it thinks

A team at the Marine Biological Laboratory (MBL) has successfully engineered an albino strain of the hummingbird bobtail squid, Euprymna berryi. This advance, reported in Current Biology, has created a nearly transparent organism that offers scientists, for the first time, clear optical access for visualizing the nervous system in a…

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Multi-omics data provide insight into the adaptation of the glasshouse plant Rheum nobile to the alpine subnival zone

Yang, Y. et al. Advances in the studies of plant diversity and ecological adaptation in the subnival ecosystem of the Qinghai-Tibet plateau. Chin. Sci. Bull. 64, 2856–2864 (2019). Article  Google Scholar  Zhang, Y., Li, B. & Zheng, D. Datasets of the boundary and area of the Tibetan plateau. Acta Geographica…

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Evolution of phenotypic disparity in the plant kingdom

Foote, M. The evolution of morphological diversity. Annu. Rev. Ecol. Syst. 28, 129–152 (1997). Article  Google Scholar  Hughes, M., Gerber, S. & Wills, M. A. Clades reach highest morphological disparity early in their evolution. Proc. Natl Acad. Sci. USA 110, 13875–13879 (2013). Article  CAS  PubMed  PubMed Central  Google Scholar  Niklas,…

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How to label only a few genes of interest in heatmap of bulk RNA-seq

How to label only a few genes of interest in heatmap of bulk RNA-seq 0 Hi all, I used this code to make a heatmap h <- Heatmap(mat.z, cluster_rows = T, cluster_columns = T, column_labels = colnames(mat.z), name = “Z-score”, row_labels = sigs.df[rownames(mat.z),]$symbol) Would you please tell me how to…

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SU6.5 2022 notes – Lecture 6 introduces the concept of DNA sequencing, see Pierce pages 590 DNA

Lecture 6 introduces the concept of DNA sequencing, see Pierce pages 590 DNA sequencing is a powerful molecular method for analyzing DNA, as it allows you to quickly determine the sequence of bases in a DNA molecule. Analogous to some of the other technologies we have explored thus far, scientist…

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Genome-resolved correlation mapping links microbial community structure to metabolic interactions driving methane production from wastewater

Lulu Island waste resource recovery ecosystem The Lulu Island WWTP operated by Metro Vancouver in Richmond, British Columbia, Canada (Longitude: −123.14498° or 123° 8’ 42” W, Latitude: 49.11491° or 49° 6’ 54” N) provides primary and secondary treatment of >30 billion liters of mixed-sourced wastewater from ~200,000 residents each year….

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How many ‘novel’ splice junctions/splice events are resonably expected from human RNA,

Hello all, I was just wondering what a reasonable percentage of ‘novel’ splice junctions/splice events is for human RNAseq data using the program junction_annotation.py. I am new to RNAseq and just running some published human RNAseq data through my pipeline in order to familiarize myself with the programs and protocols….

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Scientists Develop Novel Base Editing System

Register for free to listen to this article Thank you. Listen to this article using the player above. ✖ Want to listen to this article for FREE? Complete the form below to unlock access to ALL audio articles. In a study published in Nature Biotechnology on Aug. 28, GAO…

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Symmetric inheritance of parental histones governs epigenome maintenance and embryonic stem cell identity

The research in this study was conducted under the ethical approval of the Danish Regulatory Authority under project license 2018-15-0201-01520. Cell culture and differentiation assays WT, MCM2-2A, MCM2-R and POLE4-KO mouse ESCs used in this study were derived from the male, E14JU cell line with a 129/Ola background81. Genome editing…

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Comparative single-cell transcriptomic analysis of primate brains highlights human-specific regulatory evolution

Consensus MTG taxonomy across primates The BRAIN Initiative Cell Census Network26 generated high-resolution transcriptomic maps of the MTG in human, chimpanzee, gorilla, macaque and marmoset by applying single-nucleus transcriptomic (snRNA-seq) assays to samples isolated from between three and seven donor brains in each species (plate-based SMART-seq v4 (SSv4) for great…

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Single-cell RNA sequencing of murine hearts for studying the development of the cardiac conduction system

Mohan, R. A., Boukens, B. J. & Christoffels, V. M. Developmental origin of the cardiac conduction system: Insight from lineage tracing. Pediatr. Cardiol. 39, 1107–1114 (2018). Article  PubMed  PubMed Central  Google Scholar  van Eif, V. W. W., Devalla, H. D., Boink, G. J. J. & Christoffels, V. M. Transcriptional regulation…

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Bioinformatics Software Engineer II – Chan Lab – New York

Pay Range $96,800.00-$154,900.00 Company Overview The people of Memorial Sloan Kettering Cancer Center (MSK) are united by a singular mission: ending cancer for life. Our specialized care teams provide personalized, compassionate, expert care to patients of all ages. Informed by basic research done at our Sloan Kettering Institute, scientists across…

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Metagenomic sequencing of post-mortem tissue samples for the identification of pathogens associated with neonatal deaths

Our research fuls all relevant ethical requirements and was conducted in accordance with the Declaration of Helsinki. The parent study and the mNGS testing amendment was approved by the Human Research Ethics Committee of the University of the Witwatersrand (HREC reference number 150215). The parents of decedents had provided written…

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Director, Bioinformatics – Richmond | Mendeley Careers

Title: Director, Bioinformatics Manager: VP, Bioinformatics Department: ResearchLocation: Richmond / Remote Sangamo Therapeutics is a genomic medicine company building value with cutting-edge work across four distinct but complementary technology platforms – gene therapy, cell therapy, in vivo genome editing, and in vivo genome regulation. Using its deep scientific expertise and…

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Novel based editors developed – Drug Target Review

Researchers have developed a novel based CRISPR-free base editing system named CyDENT, which enables precise base editing. In a study published in Nature Biotechnology, GAO Caixia’s team at the Institute of Genetics and Developmental Biology (IGDB) of the Chinese Academy of Sciences, in collaboration with researchers at Qi Biodesign, has introduced…

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Metagenomic Market: Robust Expansion Expected, Anticipated CAGR of 16.5% Leading to a Market Valuation of USD 5.57 Billion by 2032

The 2022 global Metagenomic Market reached a size of USD 1.41 Billion and is projected to demonstrate a revenue CAGR of 16.5% throughout the forecast period, as stated in the latest report from Reports and Data. Metagenomic sequencing is a technique that empowers researchers to delve into the collective DNA…

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Marketing & Sales Associate | EU-Startups

Flomics is a fast-growing biotech company founded in 2018 in Barcelona, focused on the development of a revolutionary liquid biopsy solution for the early detection of cancer. Our technology is based on the analysis of cell-free RNA in blood, combining state-of-the-art genomics with sophisticated bioinformatics and machine learning techniques, to…

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Oligonucleotides Market Research Report 2023: CAGR, Industry

Oligonucleotides are short nucleic acid polymers used in research, genetic testing and forensics. Oligonucleotides are usually made up of 13 to 25 nucleotides and are designed to hybridize specifically to DNA or RNA sequences. Solid-phase clinical synthesis is used to manufacture these small bits of nucleic acid for use in…

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Functional conservation of microbial communities determines composition predictability in anaerobic digestion

Lin Q, De Vrieze J, Li C, Li J, Li J, Yao M, et al. Temperature regulates deterministic processes and the succession of microbial interactions in anaerobic digestion process. Water Res. 2017;123:134–43. Article  CAS  PubMed  Google Scholar  Coban O, De Deyn GB, van der Ploeg M. Soil microbiota as game-changers…

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Associating between results obtained after running Trinity’s run_DE_analysis.pl and genes

Associating between results obtained after running Trinity’s run_DE_analysis.pl and genes 0 Hi.How can one associate between the generic transcript names and the actual genes of the studied organism? Here are the first few lines of the output file kallisto.gene.counts.matrix.Control_vs_Serum.edgeR.DE_results (which I understood is the relevant one for differential expression analysis):…

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Adaptor sequence not present in raw paired-end reads

Adaptor sequence not present in raw paired-end reads 0 Hi, I recently downloaded a GEO dataset of paired end reads using SRAtool kit with the fastq-dump command. I performed quality check on these reads using FastQC, there were no overrepresented sequences nor detected adapter content in the raw data. I…

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Generation of inactivated IL2RG and RAG1 monkeys with severe combined immunodeficiency using base editing

Animals The gene targeting experiment was conducted on cynomolgus monkeys, which served as the experimental subjects. These monkeys were housed at Guangdong LANDAU Biotechnology Co. Ltd and TOPGENE Biotechnology Co. Ltd, where they received unrestricted access to water and were provided with a standard diet in accordance with standard care…

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The 11.6% CAGR forecasted from 2023 to 2030 provides valuable insight into the future of the Metagenomics Next Generation Sequencing Market, as determined by the market study.

The “Metagenomics Next Generation Sequencing market” report analyzes important operational and performance data so one may compare them to their own business, the businesses of their clients, or the companies of their rivals. And this report consists of 103 pages. The Metagenomics Next Generation Sequencing market is expected to grow…

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Scientist – Job September 2023

Posted: 20h ago Offer description Insmed Innovation UK Ltd, formerly Adrestia Therapeutics, is a leader in synthetic rescue therapies for genetically defined diseases and is part of Insmed Incorporated, a global biopharmaceutical company on a mission to transform the lives of patients living with serious and rare diseases. Our most…

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Zafrens, Inc. Single-cell Bioinformatics Scientist in San Diego, CA | 865232727

Zafrens, Inc., a cutting-edge biotechnology company that is trying to redefine lifesciences product development, is seeking a Single-cell Bioinformatics Scientist. This is a great opportunity for someone with a passion for bioinformatics and an interest in innovative product development to work with a team of creative and adventurous scientists who…

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6 Drug Classes and Vaccine Candidates for Hepatitis C

Hepatitis C virus (HCV) pharmacotherapeutic management is prominently led by the direct-acting antiviral (DAA) drug class, a staple of viral mitigation in patients for the last decade now. However, advances in treatment diversity and potential prevention are still pursued—despite DAAs’ significant efficacy. A recent review from Adane Adugna, of the…

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a sprouting seed in Malta

The central dogma of molecular biology is that genetic information flows in one direction, from DNA to RNA to proteins. Each ‘layer’ of information consists of a string of its sub-units. The order or sequence of these sub-units determines the contribution of the encoded information to some function in some…

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Which reference genome, DNA or cDNA and why?

Which reference genome, DNA or cDNA and why? 0 Dear Community, The goal of my study is analyze Human RNA-seq and to take the gene count for RNA-seq and then apply the DESq2. I must use the most recent genome reference, but I am unsure of which FASTA file to…

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Can we use exon- counts to calculate coverage?

Can we use exon- counts to calculate coverage? 0 Hi all, I was wondering if you could help me with this. I currently don’t have access to the bam or fastq files of the samples I’m working on from a public database. But I do have exon counts for these….

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Nanopore-based DNA and RNA Sequencing Technology Market 2023 Top Trends with Insights on Key Players , Oxford Nanopore Technologies, Geneus Tech Inc

On Orbisresearch.com, a brand-new study entitled Global “Nanopore-based DNA and RNA Sequencing Technology” Market Trends and Insights has just been released. A comprehensive evaluation of the worldwide Nanopore-based DNA and RNA Sequencing Technology market deposits mainly aimed at making to make statistical and concrete data readily accessible to the primary…

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The DNA Methylation Sequencing Market Analysis Report focuses on its Market Growth development, average selling price, and global market size is rising at a CAGR of 9.5% from 2023 – 2030

The “DNA Methylation Sequencing Market” is focused on controlling cost, and improving efficiency. Moreover, the reports offer both the demand and supply aspects of the market. The DNA Methylation Sequencing market is expected to grow annually by 9.5% (CAGR 2023 – 2030). DNA Methylation Sequencing Market Overview and Report Coverage…

Continue Reading The DNA Methylation Sequencing Market Analysis Report focuses on its Market Growth development, average selling price, and global market size is rising at a CAGR of 9.5% from 2023 – 2030

Direct PCR Reagents and Kits

This printed manual covers all aspects of GeneScan™ for the 310 Genetic Analyzer, from software installation and features to various fragment analysis (DNA sizing, genotyping) applications and software. For Research Use Only. Not for use in diagnostics procedures. The Ambion™ TaqMan PreAmp Cells-to-CT Kit makes it possible to perform gene…

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Perspectives of circular RNAs in diabetic complications from biological markers to potential therapeutic targets (Review)

Authors: Lingling Yuan Jinsheng Duan Hong Zhou View Affiliations Affiliations: Department of Endocrinology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Cardiology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei 050000, P.R. China, Department of Endocrinology, The Second Hospital of Hebei Medical University, Shijiazhuang, Hebei…

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Predicting RNA-seq coverage from DNA sequence as a unifying model of gene regulation

%PDF-1.5 % 454 0 obj <> endobj 638 0 obj <>stream application/pdf Predicting RNA-seq coverage from DNA sequence as a unifying model of gene regulation 2023-08-30T17:47:43Z LaTeX with hyperref 2023-09-02T10:05:47-07:00 2023-09-02T10:05:47-07:00 pdfTeX-1.40.24 False This is pdfTeX, Version 3.141592653-2.6-1.40.24 (TeX Live 2022) kpathsea version 6.3.4 uuid:01496bac-1dd2-11b2-0a00-f808277d8900 uuid:01496bb2-1dd2-11b2-0a00-d30000000000 endstream endobj 455 0 obj…

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TimeTalk uses single-cell RNA-seq datasets to decipher cell-cell communication during early embryo development

Curation of early-embryo development single-cell RNA-seq data sets for studying cell-cell communication To identify and study eLRs, we collected public early embryo development scRNA-seq datasets from the mouse MII-oocyte stage to the late blastocyst stage to ensure that scRNA-seq datasets represented every stage of early embryo development. In addition, to…

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Normalizer Kits for Next-Generation Sequencing

QIAGEN has launched QIAseq Normalizer Kits to provide researchers with a convenient and cost-effective method to pool different DNA libraries for best-quality results from next-generation sequencing (NGS) runs. Researchers can use QIAseq Normalizer with QIAGEN’s existing QIAseq library prep solutions to streamline NGS workflows or with a range of DNA-…

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Molecular detection and characterization of SARS-CoV-2 in cats and dogs of positive owners during the first COVID-19 wave in Brazil

Study design This was an observational study conducted between October 2020 and June 2021 in five large metropolitan areas in Brazil, belonging to four of the five different Brazilian geographical regions (Recife, Belo Horizonte, São Paulo, Campo Grande and Curitiba). Dogs and cats whose owners tested positive for SARS-CoV-2 by…

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The Transformative, Alarming Power of Gene Editing

Mitalipov is an undaunted advocate for heritable editing in humans. A hundred million people suffer from rare genetic diseases, and he believes that somatic editing can do only so much to help them. “It’s not going to alleviate transmission to the next generation,” he said. “The diseases will keep recurring…

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Nonviral Platforms Streamline Gene Therapy Delivery

By Ashleen Knutsen There is perhaps no better example of getting to the root of a problem than gene therapy. This medical treatment alters the very building blocks of life, and it has the potential to treat or cure not just genetic diseases, but cancers and infectious diseases as well….

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Top abundant deep ocean heterotrophic bacteria can be retrieved by cultivation

Staley JT, Konopka A. Measurement of in situ activities of nonphotosynthetic microorganisms in aquatic and terrestrial habitats. Annu Rev Microbiol. 1985;39:321–46. Article  CAS  PubMed  Google Scholar  Razumov AS. The direct method of calculation of bacteria in water: comparison with the Koch method. Mikrobiologija. 1932;1:131–46. Google Scholar  Kogure K, Simidu U,…

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Studocu ribosome structure notes 2 – Ribosomes are molecular machines found within cells that are

Ribosomes are molecular machines found within cells that are responsible for translating genetic information from messenger RNA (mRNA) into proteins. The structure of the ribosome is composed of two subunits, a larger 50S subunit, and a smaller 30S subunit. Together, these subunits form the functional 70S ribosome found in prokaryotic…

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CPS1 shRNA Plasmid Human) – Gentaur.com

Catalog number MBS8251016 Price Please ask Size 0,15 mg Other size please contact us to order other different size Properties Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes…

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A PhD position in clinical bioinformatics at Steno Diabetes Center Copenhagen – Leading Industry Pay at Steno Diabetes Center in 2730 Herlev

We are on the lookout for a motivated A PhD position in clinical bioinformatics at Steno Diabetes Center Copenhagen to join our incredible team at Steno Diabetes Center in 2730 Herlev.Growing your career as a Full Time A PhD position in clinical bioinformatics at Steno Diabetes Center Copenhagen is a…

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How to calculate GC content of reads that mapped to a specific gene?

How to calculate GC content of reads that mapped to a specific gene? 1 Hello, I aligned my data with STAR and I have the BAM files. I would like to calculate the GC content of reads that mapped to a specific gene. I have found this thread Gc Content…

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Comparing Budding Curves, Flow Cytometry Data, and Experimental Data

Transcript Plot the budding curves before alignment using the Python utility function by entering the command into a new cell. Next, plot the budding curves after alignment. Using the Python utility function. Use the provided plot line graph comparison in the Python utilities vial to perform line graph comparisons on…

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Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress.

Guo D, Han J, Adam BL, Colburn NH, Wang MH, Dong Z, Eizirik DL, She JX, Wang CY Proteomic analysis of SUMO4 substrates in HEK293 cells under serum starvation-induced stress. Biochem Biophys Res Commun. 2005 Dec 2;337(4):1308-18. Epub 2005 Oct 10. The substrates of SUMO4, a novel member for the…

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DNA Gel Electrophoresis Fundamentals

What Is DNA Gel Electrophoresis? DNA gel electrophoresis is a laboratory technique that separates and analyzes DNA fragments based on their size. It takes advantage of the fact that DNA molecules are negatively charged due to the phosphate groups in their structure. When an electric field is applied, these charged…

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Empowering Pharma Through Big Data: Trends & Innovations

This article presents a quarterly round-up of the latest trends in the pharmaceutical industry regarding big data. It provides an overview of recent developments in big data-related deals, talent acquisition, and patent filings within the pharmaceutical industry. Diverse applications of big data in pharmaceutical industry Pharmaceutical companies are leveraging big…

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A PhD position in clinical bioinformatics at Steno Diabetes Center

A 3-year PhD position in clinical bioinformatics is available at Steno Diabetes Center Copenhagen (SDCC). You will be a part of an exciting high-paced research environment working with precision medicine in Translational Type 2 Diabetes Research. Your main work will focus on performing various omics analyses in search of specific…

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Synthetic Biology: Future Trends & Explained

Synthetic biology, the cutting-edge fusion of industrial biotechnology and engineering principles, holds immense potential to revolutionize industries across the board. By harnessing the power of genetic molecules and genome editing, synthetic biologists aim to design and construct entirely new biological parts, devices, and systems. With this novel approach, they can…

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An eQTL Landscape of Kidney Tissue in Human Nephrotic Syndrome.

An eQTL Landscape of Kidney Tissue in Human Nephrotic Syndrome. Publication ,  Journal Article Gillies, CE; Putler, R; Menon, R; Otto, E; Yasutake, K; Nair, V; Hoover, P; Lieb, D; Li, S; Eddy, S; Fermin, D; McNulty, MT; Hacohen, N … Published in: Am J Hum Genet Expression quantitative trait loci…

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New Technology for Mapping the Tumor Ecosystem Can Improve Cancer Research

Spatially resolved transcriptomics (SRT)technology can be used to promote individualized treatment and identify therapeutic targets for patients with cancer, according to a systemic review published in Advanced Science. SRT technology is able to map tumor cells’ distribution in space, provide information on the tumor’s relationship to the surrounding microenvironment, and…

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Single-Cell Bioinformatics Software and Services Market Scope and Profit-Sources Detailed Analysis

InsightAce Analytic Pvt. Ltd. announces the release of a market assessment report on the Global Single-Cell Bioinformatics Software and Services Market By Product (Bioinformatics Software and Bioinformatics Services), Application (Oncology, Immunology, Neurology, Non-invasive Prenatal Diagnosis (NIPD), Microbiology, and Other Applications), End-user (Research and Academic Institutes, Biopharmaceutical Companies, and Others)) Trends,…

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How to download Time Calibration Data for Phylogenetic tree estimation ?

How to download Time Calibration Data for Phylogenetic tree estimation ? 1 Hi How to download time calibration data for a particular species of interest for estimating the divergence time ? Any databases ? I work with marine/fresh water organisms. Thanks Kevin gene DNA RNA • 489 views • link…

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TS Digest Issue | September 2023 | Fishing for Viruses

A mosquito makes a high-pitched buzz, followed by an unnoticeable bite at night. As a result, the Plasmodium parasite enters the human body and trips off a cascade of events that leads to malaria. To better understand the pathogenesis of this life-threatening disease,1 Steve Haase, a biologist at Duke University,…

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Potential therapeutic target for chronic kidney disease

TLTs are suggested to directly injure the proximal tubules and promote their proinflammatory property via producing cytokines such as interferon gamma and tumor necrosis factor alpha. Credit: Hiroko Uchida/Kyoto university Chronic kidney disease is a condition in which kidney function declines, causing significant health issues in patients. A previous study…

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SUMOylation of SMAD4 by PIAS1 in Conjunction with Vimentin Upregulation Promotes Migration Potential in Non-Small Cell Lung Cancer

1. Introduction Lung cancer is the most common cancer globally, with 2.21 million cases in 2020 and over 1.8 million deaths from lung cancer, making it the most common cause of cancer-related deaths [1]. In China, the estimated age-standardized incidence rate of lung cancer is as high as 34.8 per…

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Meta-analysis of microarray and RNAseq data reveal OsbZIP52 to mediate salt stress responses in sensitive, tolerant and halophyte rice varieties | CABI Agriculture and Bioscience

Anon. Human Development Report 1997. 1997. Bailey TL, Johnson J, Grant CE, Noble WS. The MEME suite. Nucleic Acids Res. 2015;43(W1):W39-49. doi.org/10.1093/NAR/GKV416. Article  CAS  PubMed  PubMed Central  Google Scholar  Balchin D, Hayer-Hartl M, Hartl FU. In vivo aspects of protein folding and quality control. Science. 2016;353(6294):aac4354. doi.org/10.1126/science.aac4354. Article  CAS  PubMed …

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RNAseq (Deseq2 and Dexseq2) analysis on two time points with expected same effect

RNAseq (Deseq2 and Dexseq2) analysis on two time points with expected same effect 1 @71c6768f Last seen 7 hours ago United States Hello, We are analyzing an RNA-seq experiment that was performed in cases and controls across two time points. We are interested in finding differentially expressed genes (DESeq2) and…

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The Lifecycle of Ribosomal RNA in Bacteria

Review In: RNA Damage and Repair. Cham (CH): Springer; 2021 Jul. Chapter 2. Affiliations Expand Affiliations 1 School of Biological and Chemical Sciences, Queen Mary University of London London, UK 2 Corresponding author: c.engl@qmul.ac.uk Free Books & Documents Item in Clipboard Review Maria Grazia Giuliano et al. Free Books & Documents…

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Real-time PCR Reagents for so-called superbugs: Candida auris Detection

Candida auris is a form of yeast that can be fatal to fragile hospital and nursing home patients. Candida auris usually does not harm healthy people, but some are so-called super bacteria that are resistant to all three types of antibiotics for fungal infections treatment. The Candida auris Detection Real-time…

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Metagenomics Market Is Estimated To Register Robust Revenue CAGR Over The Forecast Period

PRESS RELEASE Published August 31, 2023   Metagenomics is a field of molecular biology that involves studying the genetic material (DNA or RNA) extracted directly from environmental samples, such as soil, water, or biological organisms, without the need for isolating and cultivating individual organisms. The goal of metagenomics is to…

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Trajectory of hiPSCs derived neural progenitor cells differentiation into dermal papilla-like cells and their characteristics,Scientific Reports

Dermal papilla cells (DPCs) play roles in key functions of the epidermis such as hair generation. The use of human induced pluripotent cells (hiPSCs) makes it possible to obtain DP-like cells and study the molecular mechanisms of DPC development during embryogenesis. In this work, we studied the phenotypic trajectory of…

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Multiplexed transcriptomic profiling of the fate of human CAR T cells in vivo via genetic barcoding with shielded small nucleotides

Wagner, A., Regev, A. & Yosef, N. Revealing the vectors of cellular identity with single-cell genomics. Nat. Biotechnol. 34, 1145–1160 (2016). Article  CAS  PubMed  PubMed Central  Google Scholar  Gehring, J., Hwee Park, J., Chen, S., Thomson, M. & Pachter, L. Highly multiplexed single-cell RNA-seq by DNA oligonucleotide tagging of cellular…

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Effects of host species on microbiota composition in Phlebotomus and Lutzomyia sand flies | Parasites & Vectors

Maroli M, Feliciangeli MD, Bichaud L, Charrel RN, Gradoni L. Phlebotomine sandflies and the spreading of leishmaniases and other diseases of public health concern. Med Vet Entomol. 2013;27:123–47. CAS  PubMed  Google Scholar  Akhoundi M, Kuhls K, Cannet A, Votypka J, Marty P, Delaunay P, et al. Historical overview of the…

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How To Optimize Your Electroporation

Electroporation has transformed the way in which scientists work, allowing the delivery of genetic material, proteins, drugs or other molecules into mammalian, insect, fungal, plant and bacterial cells. Nevertheless, as with all laboratory techniques, it is not without its fair share of pitfalls and disappointments.   Therefore, it is important…

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Avoiding false discoveries: Revisiting an Alzheimer’s disease snRNA-Seq dataset

Reviewer #1 (Public Review): Murphy, Fancy and Skene performed a reanalysis of snRNA-seq data from Alzheimer Disease (AD) patients and healthy controls published previously by Mathys et al. (2019), arriving at the conclusion that many of the transcriptional differences described in the original publication were false positives. This was achieved…

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Emergence of putative energy parasites within Clostridia revealed by genome analysis of a novel endosymbiotic clade

Cryan JF, O’Riordan KJ, Cowan CSM, Sandhu KV, Bastiaanssen TFS, Boehme M, et al. The microbiota-gut-brain axis. Physiol Rev. 2019;99:1877–2013. Article  CAS  PubMed  Google Scholar  Lopetuso LR, Scaldaferri F, Petito V, Gasbarrini A. Commensal clostridia: leading players in the maintenance of gut homeostasis. Gut Pathog. 2013;5:e23. Article  Google Scholar  Collins…

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Functional annotation and comparative genomics analysis of Balamuthia mandrillaris reveals potential virulence-related genes

Levinson, S. et al. Balamuthia mandrillaris brain infection: A rare cause of a ring-enhancing central nervous system lesion. Illustrative case. J. Neurosurg Case Lessons 3, 25 (2022). Google Scholar  Wu, X. et al. Diagnosing Balamuthia mandrillaris encephalitis via next-generation sequencing in a 13-year-old girl. Emerg. Microbes Infect. 9, 1379–1387 (2020)….

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GMP Plasmid DNA Manufacturing Market Latest Report with Forecast to 2031

InsightAce Analytic Pvt. Ltd. has announced the publication of a market research report titled Global GMP Plasmid DNA Manufacturing Market By Product(Viral Vectors(Retroviral, Adenoviral, Lentiviral, Adeno-Associated, Others), Plasmid DNA, Non-viral, Lipid/polymer, Electroporation, Nanoparticles, Others), Types (Pre-Clinical Therapeutics, Clinical Therapeutics, Marketed Therapeutics), Application(Gene Therapy, DNA Vaccines, Immunotherapy)) Market Outlook and Industry…

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IdentifySensors Biologics, East West Manufacturing Partner on Rapid Saliva-Based Biosensor MDx

NEW YORK – IdentifySensors Biologics announced on Tuesday that it has formed a partnership with East West Manufacturing to manufacture its medical diagnostic devices. Cleveland, Ohio-based IdentifySensors is developing biosensors to detect DNA and RNA from infectious pathogens such as SARS-CoV-2, influenza, respiratory syncytial virus, norovirus, rotavirus, adenovirus, and pathogens…

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Purinergic neurotransmission receptor P2X4 silencing alleviates intracerebral hemorrhage-induced neuroinflammation by blocking the NLRP1/Caspase-1 pathway

Mouse ICH model The 8-week-old male CD1 mice (Charles River, Wilmington, USA) weighing 30–32 g were used for the establishment of the ICH model. In the brain tissue surrounding the hematoma in the right basal ganglia region, collagenase was injected into the right basal ganglia to induce ICH. Then, all mice…

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Small Study of Gene Editing to Treat Sickle Cell Disease

An early-stage study of a CRISPR-Cas9-based treatment coaxes cells to produce fetal hemoglobin and decreases painful symptoms of sickle cell disease, researchers report in a Novartis Pharmaceuticals-sponsored study appearing in the New England Journal of Medicine. Sickle cell disease is caused by mutations in the HBB gene, which encodes a…

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Lactobacillus crispatus+gasseri+jensenii + Gardnerella vaginalis + Atopobium vaginae rRNA [Presence] in Vaginal fluid by NAA with probe detection – 94420-7

LOINC Code LOINC code 94420-7 name Lactobacillus crispatus+gasseri+jensenii + Gardnerella vaginalis + Atopobium vaginae rRNA [Presence] in Vaginal fluid by NAA with probe detection description Qualitative result for bacterial vaginosis (BV) based on the detection and quantitation of ribosomal RNA from bacteria associated with bacterial vaginosis (BV), including Lactobacillus…

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