Tag: rRNA

In-Depth Analysis of Bacillus anthracis 16S rRNA Genes and Transcripts Reveals Intra- and Intergenomic Diversity and Facilitates Anthrax Detection

Various ratios of 16S-BA/BC-alleles constitute possible explanations for differences in FISH signals of cells of diverse B. anthracis strains (Fig. 1). Indeed, we found a significant correlation between 16S-BA/BC-allele ratios in sequenced genomes and mean intensities of the Cy3 FISH signals targeting the16S-BA-allele (tested with the cor.test function in R, Pearson’s…

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WLQ #5.doc – BIOL/BNFO 541 Wet Lab Quizzes 5 Protocol 3 and 6 3 Agarose gel electrophoresis 6 Cloning 16S rRNA gene PCR fragments into pGEM u00ae-T Easy

BIOL/BNFO 541 Wet Lab Quizzes # 5, Protocol 3 and 6 – March 15, 2019 3. Agarose gel electrophoresis 6. Cloning 16S rRNA gene PCR fragments into pGEM ® -T Easy plasmid vector Deadline: March 14 , 2019, Thursday, midnight sharp! No copying and pasting! Use your own words. Mario…

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Collateral cleavage of 28s rRNA by RfxCas13d causes death of mice

Abstract Summary The CRISPR-Cas13 system is an RNA-guided RNA-targeting system, and has been widely used in transcriptome engineering with potentially important clinical applications. However, it is still controversial whether Cas13 exhibits collateral activity in mammalian cells. Here, we found that knocking down gene expression using RfxCas13d in the adult brain…

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Systematic mapping of rRNA 2′-O methylation during frog development and involvement of the methyltransferase Fibrillarin in eye and craniofacial development in Xenopus laevis

Ribosomes are essential nanomachines responsible for protein production. Although ribosomes are present in every living cell, ribosome biogenesis dysfunction diseases, called ribosomopathies, impact particular tissues specifically. Here, we evaluate the importance of the box C/D snoRNA-associated ribosomal RNA methyltransferase fibrillarin (Fbl) in the early embryonic development of Xenopus laevis. We…

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Intraspecies variability of the 16S rRNA gene of the soil bacteria Acinetobacter lwoffii and Paenibacillus taichungensis

Anastasiya Nechayeva, Konstantin Boyarshin*, Olga Bespalova, Viktoriia Iatsenko, Evgeniy Seliverstov, Violetta Klyueva and Olesya Makanina The functioning of well-studied key groups of soil microorganisms depends on the microbial ecosystem in which they function in interaction with species belonging to other ecological groups. An analysis of the composition of the soil…

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Insight into the symbiotic lifestyle of DPANN archaea revealed by cultivation and genome analyses

Significance The DPANN superphylum is a grouping of symbiotic microorganisms categorized based on their genomic contents and a few examples of cultivation experiments. Although the genome information of DPANN archaea is increasing year by year, most of them have remained uncultivated, limiting our knowledge of these organisms. Herein, a thermoacidophilic…

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EXAM 2.docx – EXAM 2 1 In the Stringent Response bacterial rRNA tRNA and protein synthesis temporarily cease in response to depleted nutrients in the

EXAM 21.In the Stringent Response, bacterial rRNA, tRNA, and protein synthesis temporarilycease in response to depleted nutrients in the cell. The stringent response is initiated inthe cell due to:a.an excess of aminoacyl-tRNA that accumulates due to a block in translationb.accumulation of cyclic-AMP in the cellc.metabolite inhibition of isoenzyme function in…

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ASM1584570v1 – Genome – Assembly

##Genome-Annotation-Data-START##Annotation Date::05/22/2015 10:24:41Annotation Method::Best-placed reference protein set; GeneMarkS+Annotation Pipeline::NCBI Prokaryotic Genome Annotation PipelineAnnotation Provider::NCBIFeatures Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_regionAnnotation Software revision::2.10 (rev. 463717)Genes::5,665CDS::5,280Pseudo Genes::271CRISPR Arrays::4rRNAs::32 (5S, 16S, 23S)tRNAs::82ncRNA::1Frameshifted Genes::68##Genome-Annotation-Data-END## Read more here: Source link

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Natural Chromosome-Chromid Fusion across rRNA Operons in a Burkholderiaceae Bacterium

Chromids (secondary chromosomes) in bacterial genomes that are present in addition to the main chromosome appear to be evolutionarily conserved in some specific bacterial groups. In rare cases among these groups, a small number of strains from Rhizobiales and Vibrionales were shown to possess a naturally fused single chromosome that…

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16S rRNA, One of the Most Important rRNAs

The 16S rRNA and 18S rRNA genes are the most frequently used targets for bacteria/archaea and eukaryotes, respectively. Based on the diversity of ribosomal RNA sequences, one can explore the structure of the microbiome in terms of presence and relative abundance. prok. -30S -50S What are the functional components of…

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Microbial identification of bovine milk isolates compared between conventional culture, MALDITOF and 16S rRNA

Microbial identification of bovine milk isolates compared between conventional culture, MALDITOF and 16S rRNA 21st International Conference on Food Technology & Processing October 04-06, 2018 | London, UK David J Wilson, John R Middleton, Pamela R F Adkins and Gregory M Goodell Utah State University, USAUniversity of Missouri, USAThe Dairy…

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There are 4 types of RNA: mRNA, rRNA, sRNA, and tRNA.

Home There are 4 types of RNA: mRNA, rRNA, sRNA, and tRNA. There are 4 types of RNA: mRNA, rRNA, sRNA, and tRNA. This is a List of Available Answers Options : False True The best answer is A. False. Reported from teachers around the world. The correct answer to ❝There…

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Microbiota and Body Composition During the Period of Complementary Feeding

In this study,  scientists aimed at investigating the relationships between food category consumption, fecal microbial profile, and body composition throughout the supplemental feeding phase. In a cohort of 50 babies aged 6 to 24 months, the diet was examined using a quantitative food frequency questionnaire, fecal microbiota profile was analyzed using…

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Omics advanced 07 | transcriptome mRNA | miRNA | lncrna

A brief introduction mRNA,miRNA,lncRNA And circRNA Principle of genome sequencing 、 Routine analysis of Shengxin . Catalog Introduction to transcriptome sequencing mRNA|miRNA|lncRNA|circRNA Introduction to analysis mRNA Group miRNA Group lncRNA Group circRNA Group mRNA|miRNA|lncRNA|circRNA Correlation analysis Reference Introduction to transcriptome sequencing Transcriptome (Transcriptome) Is a single or group of cells…

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What is RNAcentral? | RNAcentral

RNAcentral is a database of non-coding RNA sequences that aggregates ncRNA data from over 40 member resources known as Expert Databases.1 Non-coding RNAs Similar to mRNAs, non-coding RNAs (ncRNAs) are transcribed from DNA but are not translated into proteins. NcRNAs are found in all organisms and have a broad range…

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Phylogenomic analysis confirms polyphyly of Leptospermum and delineates five major clades that warrant generic recognition – Binks – – TAXON

Leptospermum is an ecologically and economically important genus with a long unresolved taxonomic issue concerning polyphyly, as indicated from early molecular analysis on two chloroplast regions. To resolve this, we used genome skimming to obtain high-copy chloroplast and nuclear ribosomal DNA for a comprehensive phylogenomic analysis of 110 accessions comprising…

Continue Reading Phylogenomic analysis confirms polyphyly of Leptospermum and delineates five major clades that warrant generic recognition – Binks – – TAXON

ddPCR allows 16S rRNA gene amplicon sequencing of very small DNA amounts from low-biomass samples | BMC Microbiology

1. Lane DJ, Pace B, Olsen GJ, Stahl DA, Sogin ML, Pace NR. Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. Proc Natl Acad Sci U S A. 1985;82(20):6955–9. PubMed  PubMed Central  CAS  Google Scholar  2. Vos M, Quince C, Pijl AS, de Hollander M, Kowalchuk GA. A…

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An rRNA modification benefitting E. Coli under stress

The Polacek in collaboration with the Limbach lab investigated the role of the ho5C2501 23S rRNA modification of the E. Coli ribosome and found out that under oxidative stress benefits the bacteria by reducing protein synthesis. Their findings have been published in an article in Nucleic Acids Research “Dynamic 23S…

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Dynamic 23S rRNA modification ho5C2501 benefits Escherichia coli under oxidative stress

doi: 10.1093/nar/gkab1224. Online ahead of print. Affiliations Expand Affiliations 1 Department of Chemistry, Biochemistry and Pharmaceutical Sciences, University of Bern, 3012 Bern, Switzerland. 2 Graduate School for Cellular and Biomedical Sciences, University of Bern, 3012 Bern, Switzerland. 3 Institute of Organic Chemistry, Center for Molecular Biosciences Innsbruck, University of Innsbruck, 6020 Innsbruck, Austria. 4 Rieveschl Laboratories…

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90S Pre-Ribosomes Include the 35S Pre-rRNA, the U3 snoRNP, and 40S Subunit Processing Factors but…

Title Authors Grandi, Paola Rybin, Vladimir Baßler, Jochen Petfalski, Elisabeth Strauß, Daniela Marzioch, Martina Schäfer, Thorsten Kuster, Bernhard Tschochner, Herbert Tollervey, David Hurt, Ed show all authors [1 – 12] Published in Molecular cell. 2002, vol. 10, no. 1, p. 105-115 Identifiers Full text Article (Published version) (1.3 MB) –…

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16S rRNA gene amplicon sequence data from sunflower endosphere bacterial community

doi.org/10.1016/j.dib.2021.107636Get rights and content Abstract Insights into plant endosphere bacterial diversity and exploration of their bioincentives in the formulation of biofertilizers promise to avert ecological disturbances. Here, we presented the sequence dataset of the endophytic bacterial community from the roots and stems of sunflower obtained from farmlands in Itsoseng and…

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Comparative analysis of circular RNA enrichment methods

The circRNAs sequencing results vary due to the different enrichment methods and their performance is needed to systematic comparison. This study investigated the effects of different circRNA enrichment methods on sequencing results, including abundance and species of circRNAs, as well as the sensitivity and precision. This experiment was carried out…

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A Study Of Molecular Detection of Helicobacter pylori IceA gene in Gastric biopsy from Endoscopic patients in AL Faisal and AL Ribat Hospitals in Khartoum State

Abstract: Colonization with Helicobacter pylori known to be cause of variety of clinical consequences includes gastritis or even gives rise to severe and potentially fatal complications, and different studies suggested that there is a relationship between the clinical outcomes and some virulence gene that H.pyloi has. The aim of this…

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18S gene not present in genome assembly?

18S gene not present in genome assembly? 0 I am designing PCR primers to amplify a region of the 18S rRNA gene of Penicillium expansum. As the template for primer design, I use the consensus sequence of a multiple sequence alignment of 18S sequences obtained from the SILVA database. When…

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16s rRNA Sequencing Meta-analysis Reconstruction Tool (using mothur).

16SMaRT is a bioinformatics analysis pipeline for 16s rRNA gene sequencing data. 16SMaRT is a “one-click” solution towards performing microbial community analysis of amplicon sequencing data. 16SMaRT aims to be your go-to solution for your next microbiome/metagenomics project. The primary objective of 16SMaRT analysis is to determine what genes are…

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There are 4 rRNAs produced to make both subunits of the ribosome. How many transcripts are required to produce these 4 rRNAs? a. 1. b. 2. c. 3. d. 4.

Question: There are {eq}4 {/eq} rRNAs produced to make both subunits of the ribosome. How many transcripts are required to produce these {eq}4 {/eq} rRNAs? a. {eq}1 {/eq}. b. {eq}2 {/eq}. c. {eq}3 {/eq}. d. {eq}4 {/eq}. Ribosomes Ribosomes perform the critical action of protein synthesis. Often found in the…

Continue Reading There are 4 rRNAs produced to make both subunits of the ribosome. How many transcripts are required to produce these 4 rRNAs? a. 1. b. 2. c. 3. d. 4.

Using reverse-complement or just complement of SILVA database to filter rRNA from metatranscriptomics

Using reverse-complement or just complement of SILVA database to filter rRNA from metatranscriptomics 0 I want to use SILVA database to filter rRNA from metatranscriptomics. The nucleobases in the SILVA fasta file are A, U, C and G. Therefore, before indexing the SILVA fasta file, should I get the reverse-complement…

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get rRNA FASTA file for a particular bacteria

get rRNA FASTA file for a particular bacteria 0 Hey all, I was trying to find a way to get all rRNA (5S, 16S and 23S) FASTA sequences for a particular bacteria (B. thetaiotaomicron VPI-5482, which is the type strain). I wanted this file so that I could use something…

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ASM648341v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI Annotation Date::01/22/2018 18:06:09 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline Annotation Method::Best-placed reference protein set; GeneMarkS+ Annotation Software revision::4.3 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::7,178 CDS (total)::7,112 Genes (coding)::6,886 CDS (coding)::6,886 Genes (RNA)::66 rRNAs::1, 1, 1 (5S, 16S, 23S) complete rRNAs::1, 1, 1 (5S, 16S,…

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ASM296653v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI RefSeq Annotation Date::03/19/2021 18:16:01 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method::Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision::5.1 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::1,877 CDSs (total)::1,821 Genes (coding)::1,767 CDSs (with protein)::1,767 Genes (RNA)::56 rRNAs::2, 2, 2 (5S, 16S, 23S) complete rRNAs::2, 2,…

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Complete mitochondrial genome sequencing of Oxycarenus laetus (Hemiptera: Lygaeidae) from two geographically distinct regions of India

Genome organization and structure of O. laetus The MiSeq data of BTI and CBE samples of O. laetus was submitted to the NCBI SRA database under the accession numbers SRR11024516 and SRR11024517, respectively. The study was done to understand whether there exists a different species of the genus Oxycarenus similar…

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Running SortMeRNA on Multiple Files

Running SortMeRNA on Multiple Files 0 Hi all, I am VERY new to SortMeRNA (I’m a PhD student taking a bioinformatics class that has been very poorly taught). I have 27 paired samples for a total of 54 samples named like this: SRR13711719_1_val_1.fq SRR13711719_2_val_2.fq. So the format is _1_val_1.fq and…

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(PDF) Analysis of 16S rRNA environmental sequences using MEGAN

(PDF) Analysis of 16S rRNA environmental sequences using MEGAN | Suparna Mitra – Academia.edu Academia.edu uses cookies to personalize content, tailor ads and improve the user experience.By using our site, you agree to our collection of information through the use of cookies.To learn more, view our Privacy Policy. × Log in…

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Microchip Analysis – an overview

33.3.1.3 Oil Field Microbiology: Asia Oilfields in Russia and China have been extensively studied to determine microbial ecology as well as understand the microbial community for bioprospecting aspects. Molecular approaches as well as radioisotopic activity measurements from formation water of a high temperature oil reservoir in Russia (Samotlor) indicated enrichment…

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High-throughput sequencing of 16S rRNA gene analysis reveals novel taxonomic diversity among vaginal microbiota in healthy and affected sows with endometritis

Available online 7 December 2021 doi.org/10.1016/j.rvsc.2021.12.003Get rights and content Highlights • Corynebacterium_1, Clostridium_sensu_stricto_1, Porphyromonas, Anaerococcus, Streptococcus, and Bacteroides were the core microbiota in all healthy sows. • V3-V4 region of the 16S rRNA gene sequence and full-length 16S rRNA analysis confirmed that Burkholderia was a dominant genus in endometritis sows…

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(PDF) Analysis of 16S rRNA environmental sequences using MEGAN | Suparna Mitra

(PDF) Analysis of 16S rRNA environmental sequences using MEGAN | Suparna Mitra – Academia.edu Academia.edu uses cookies to personalize content, tailor ads and improve the user experience. By using our site, you agree to our collection of information through the use of cookies. To learn more, view our Privacy Policy. ×…

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MitoVisualize: A resource for analysis of variants in human mitochondrial RNAs and DNA

Abstract We present MitoVisualize, a new tool for analysis of the human mitochondrial DNA (mtDNA). MitoVisualize enables visualization of: (1) the position and effect of variants in mitochondrial transfer RNA (tRNA) and ribosomal RNA (rRNA) secondary structures alongside curated variant annotations, (2) data across RNA structures, such as to show…

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ASM350094v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI Annotation Date::04/27/2018 21:42:42 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline Annotation Method::Best-placed reference protein set; GeneMarkS+ Annotation Software revision::4.5 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::3,542 CDS (total)::3,498 Genes (coding)::3,451 CDS (coding)::3,451 Genes (RNA)::44 tRNAs::40 ncRNAs::4 Pseudo Genes (total)::47 Pseudo Genes (ambiguous residues)::2 of 47 Pseudo…

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ASM314399v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI Annotation Date::05/15/2018 16:18:51 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline Annotation Method::Best-placed reference protein set; GeneMarkS+ Annotation Software revision::4.5 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::1,893 CDS (total)::1,839 Genes (coding)::1,782 CDS (coding)::1,782 Genes (RNA)::54 rRNAs::3, 1, 1 (5S, 16S, 23S) complete rRNAs::3, 1 (5S, 16S) partial…

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ASM1227490v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI RefSeq Annotation Date::02/09/2021 05:00:21 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method::Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision::5.0 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::4,608 CDSs (total)::4,469 Genes (coding)::4,408 CDSs (with protein)::4,408 Genes (RNA)::139 rRNAs::10, 9, 9 (5S, 16S, 23S) complete rRNAs::10, 9,…

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ASM298219v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI RefSeq Annotation Date::01/12/2021 08:24:12 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method::Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision::5.0 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::5,192 CDSs (total)::5,077 Genes (coding)::4,870 CDSs (with protein)::4,870 Genes (RNA)::115 rRNAs::9, 8, 8 (5S, 16S, 23S) complete rRNAs::9, 8,…

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Whole genome sequencing and RNA sequencing data analysis for a bacterial strain

Whole genome sequencing and RNA sequencing data analysis for a bacterial strain 1 Hello, I am new RNA sequencing work. I have raw fastq files (from extracted RNA and DNA both) for bacteria “Paucibacter toxinivorans strain IM4”. I am not able to find whole reference genome for the same bacteria….

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Mutational Analysis of Mitochondrial tRNA Genes

Introduction Diabetes is a very complex disease characterized by the presence of chronic hyperglycemia. Clinically, insulin-dependent type 1 and non-insulin-dependent type 2 are the main types of diabetes. Among them, type 2 diabetes mellitus (T2DM, [MIM125853]) is a common endocrine disorder affecting approximately 10% of adult population.1 In most cases,…

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ASM212806v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI RefSeq Annotation Date::10/12/2020 21:58:49 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method::Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision::4.13 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::767 CDSs (total)::724 Genes (coding)::684 CDSs (with protein)::684 Genes (RNA)::43 rRNAs::1, 1, 1 (5S, 16S, 23S) complete rRNAs::1, 1,…

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probable dimethyladenosine transferase-like, maker-scaffold153_size302544-snap-gene-2.18 (gene) Tigriopus kingsejongensis

Associated RNAi Experiments Homology BLAST of probable dimethyladenosine transferase-like vs. L. salmonis genes Match: EMLSAG00000006273 (supercontig:LSalAtl2s:LSalAtl2s341:673186:674124:1 gene:EMLSAG00000006273 transcript:EMLSAT00000006273 description:”augustus_masked-LSalAtl2s341-processed-gene-6.3″) HSP 1 Score: 484.567 bits (1246), Expect = 2.083e-174Identity = 227/310 (73.23%), Postives = 259/310 (83.55%), Query Frame = 0 Query: 9 KVRKTGSGMSTVEAAGSGGGGQQGMVFNTGLGQHILKNPLVVQSIIDKAALRSTDVVLEIGPGTGNLTVRALEKCKKLIACEVDPRMVAELQKRVQGTHFQSKLQIMVGDVIKTDLPFFDACVANVPYQISSPLVFKLLLHRPFFRCAVLMFQREFAQRLVAKPGDKLYCRLSINTQLLARVDHVMKVGKGNFRPPPKVESSVVRIEPRNPPPPINFKEWDGLTRVAFVRKNKTLGAAFNQTTVLMMLEKNYRVHLSLADEPVPEKIDIKSIIETVLAEIAFKEKRARSMDIDDFMKLLHAFNAKGIHFV 318 KV+ T + GG+QG+VFNT LGQHILKNP VV…

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ASM238634v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI RefSeq Annotation Date::06/05/2020 15:45:56 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline (PGAP) Annotation Method::Best-placed reference protein set; GeneMarkS-2+ Annotation Software revision::4.11 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::1,994 CDSs (total)::1,917 Genes (coding)::1,885 CDSs (with protein)::1,885 Genes (RNA)::77 rRNAs::4, 4, 4 (5S, 16S, 23S) complete rRNAs::4, 4,…

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RNAseq data mapping mostly to intergenic regions

RNAseq data mapping mostly to intergenic regions 1 Hi everyone, I am working on a project trying to do RNAseq on highly degraded formalin-fixed brain tissues. We extracted RNA using Qiagen FFPE RNeasy kit and the sequencing company suggested to do rRNA depletion method to prepare libraries. We sequenced with…

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Still Not Use QIIME2 for Amplicon Analysis? You’re OUT!

I want to share with you a powerful tool named QIIME 2. It’s handy for Amplicon analysis. What is QIIME 2? The Quantitative Insights Into Microbial Ecology (QIIME) microbiome bioinformatics platform has supported many microbiome studies and gained a broad user and developer community. QIIME 2™(qiime2.org) is a new version…

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How much does the DNA extraction route impact the results of microbiome research?

Comparing DNA extractions for microbiome studies. Credit: Tess Deyett Let’s face it, we are a results-driven society. Too focused on the outcome, people don’t often think about the “how.” For instance, did you think about “how” you got to work this morning or did you just get there? In microbiome…

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chloramphenicol resistance

Using these strains, it is possible to integrate plasmids that carry the complementary ΦC31 att site onto the chromosome (attP, in this figure). Because the mtDNA codes for key OXPHOS polypeptides, genetic alterations in the mtDNA will affect energy metabolism. Proc Natl Acad Sci U S A. 1969 Jun;98(3):1248-57 eCollection…

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Error while running MEGAX

Error while running MEGAX 0 I am running the latest version of MEGA i.e MEGAX. I am trying to run the command megacc but it is throwing an error. My sequences.fas file contains the trimmed multiple sequence alignment (MSA) of 16S rRNA sequences of 1403 organisms in a fasta format….

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Emergence of the Coexistence of mcr-1, blaNDM-5, and blaCTX-M-55 in Kl

Introduction Klebsiella pneumoniae (K. pneumoniae) is an opportunistic pathogen and the leading cause of healthcare-associated infections.1 Multidrug-resistant (MDR) K. pneumoniae isolates are rapidly spreading, thus limiting the choice of antimicrobial agents for empiric treatment of infections caused by these microorganisms; hence, this is a public health challenge.2 Polymyxins are last-resort…

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Multiform antimicrobial resistance from a metabolic mutation

Abstract A critical challenge for microbiology and medicine is how to cure infections by bacteria that survive antibiotic treatment by persistence or tolerance. Seeking mechanisms behind such high survival, we developed a forward-genetic method for efficient isolation of high-survival mutants in any culturable bacterial species. We found that perturbation of…

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Global phylogenomic analyses of Mycobacterium abscessus provide context for non cystic fibrosis infections and the evolution of antibiotic resistance

1. Lee, M.-R. et al. Mycobacterium abscessus complex infections in humans. Emerg. Infect. Dis. 21, 1638–1646 (2015). CAS  PubMed  PubMed Central  Google Scholar  2. Prince, D. S. et al. Infection with Mycobacterium avium complex in patients without predisposing conditions. N. Engl. J. Med. 321, 863–868 (1989). CAS  PubMed  Article  Google…

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ASM287662v1 – Genome – Assembly

##Genome-Annotation-Data-START## Annotation Provider::NCBI Annotation Date::08/10/2016 16:40:10 Annotation Pipeline::NCBI Prokaryotic Genome Annotation Pipeline Annotation Method::Best-placed reference protein set; GeneMarkS+ Annotation Software revision::3.3 Features Annotated::Gene; CDS; rRNA; tRNA; ncRNA; repeat_region Genes (total)::3,675 CDS (total)::3,608 Genes (coding)::3,557 CDS (coding)::3,557 Genes (RNA)::67 rRNAs::2, 1, 1 (5S, 16S, 23S) complete rRNAs::1, 1, 1 (5S, 16S,…

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Find the copy number of marker genes and the number of them that results in drug resistance?

Find the copy number of marker genes and the number of them that results in drug resistance? 0 Hi, I want to find a copy number for different gene marker and also investigate if I have certain number of them, will it results in resistance to a drug? For example,…

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Helicobacter pylori in Pakistan | IDR

Introduction Helicobacter pylori, an infectious agent of human chronic active gastritis and also associated with other gastric ailments such as peptic ulcer, gastric cancer, and mucosa-associated lymphoid tissue lymphoma.1 Clarithromycin is considered the best option among other antibiotics for the management of this bacterial infection.2 However, increasing resistance against this…

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A Combined Digital PCR and Next Generation DNA-Sequencing Based Approach for Tracking Nearshore Pollutant Dynamics Along the Southwest United States/Mexico Border

doi: 10.3389/fmicb.2021.674214. eCollection 2021. Affiliations Expand Affiliations 1 Southern California Coastal Water Research Project, Costa Mesa, CA, United States. 2 BioTechnology Institute, University of Minnesota Twin Cities, Saint Paul, MN, United States. 3 Department of Soil, Water, and Climate, University of Minnesota Twin Cities, Saint Paul, MN, United States. 4…

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Downloading 16S sequences using JGI portal

Just in case anyone is facing a similar problem 3 yrs later: You could locate this study on IMG and then, using the following description from the 2021 IMG publication, access just these sequences: As an example, consider a query to retrieve all 16S rRNA genes longer than 500nt in…

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Controlling Banana Xanthomonas Wilt Disease in East Africa

© Olena Danileiko Experts Leena Tripathi, Jaindra Nath Tripathi and Richard Goodman, provide a compelling analysis of controlling Banana Xanthomonas Wilt Disease in East Africa By way of an introduction, banana (Musa spp.) is one of the most common fruits worldwide. However, it is an important staple crop in the…

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MAKER genome annotation error with SNAP ab initio prediction

I am trying to do a second round of maker genome annotation with ab initio prediction by snap. The error I am getting is as follows: error: unknown command “genome.hmm”, see ‘snap help’. ERROR: Snap failed –> rank=NA, hostname=bioinformatics ERROR: Failed while preparing ab-inits ERROR: Chunk failed at level:0, tier_type:2…

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Sortmerna error

Sortmerna error 0 Hello, I am facing this error in one of my files during sortmerna [split:646] ERROR: Failed deflating readstring: @A00489:986:HGYHKDRXY:1:2123:9824:27242 1:N:0:CAGTGCTT+ACCTGGAA CGGCTGCCTCTCAGGGGCGGTGGGGGGCGCGGCCGGCAGCGGCCCGCGGGGCGCGGGGGGCACCGAGTCGCTGCTGAAGTCCAGCAGCGGTGCGGCGGCGGGGGGCACCGGAGCCGCGGACAGCCCGGCTGCGGGCTTCCTCTCCAGCAC + FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF::FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF,FFFFFFFFFFFFFFFF:F:FFFFFFFFF I have used the tool before many times and in this run all the other samples are also working without errors. The md5sum doesn’t…

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