Tag: RT-qPCR

Capturing proteins associated with SARS-CoV-2 virions To identify host cell proteins potentially present in extracellular SARS-CoV-2 virions, viral particles were produced from two lung epithelial cell models, A549 cells overexpressing ACE2 receptor (A549-ACE2) and Calu-3 cells that naturally express ACE2 receptor and TMPRSS2. Following infection with the D614G SARS-CoV-2 strain32,…

Expression pattern of miR-195 during embryonic development We analyzed the expression pattern of miR-195 by in situ hybridization at two distinct developmental stages in mouse embryos. Whole-mount in situ hybridization at E10.5, demonstrated a wide expression of miR-195 along the entire embryo, including the central nervous system, the cardiopharyngeal area,…

This protocol enables the impact of prophages on their hosts to be revealed. Bacterial cultures are synchronized using conditions that best support the lysogenic state, limiting spontaneous induction. RT-qPCR unequivocally distinguishes prophage-restricted genes and those uncoupled from phage control from those that are expressed during the lytic replication cycle. Prophages…

Objectives: Hepatitis E virus (HEV) is the leading cause of acute viral hepatitis worldwide. HEV RNA detection is the gold standard for HEV infection diagnosis and PCR methods are commonly used but are usually time-consuming and expensive, resulting in low detection efficiency and coverage, especially in low-income areas. Here, we…

Loss of ASH2L prevents cell proliferation We have studied the molecular and cellular consequences of Ash2l loss in mouse embryo fibroblasts (MEFs) with floxed Ash2l alleles and an inducible Cre-ER recombinase (iMEF-Ash2lfl/fl-Cre-ER). While the knockout of Ash2l was rapid, the downstream effects, including the decrease in promoter-associated H3K4me3, altered gene…

Identification of DRGs in PCa Figure 1a showed the flow chart of this study. To explore the role of DRGs in PCa, we analyzed the gene expression profiles of these 15 DRGs in PCa patients. As shown in Fig. 1b, for ACTN4, TLN1, IQGAP1, CD2AP, FLNA, MYH9, MYL6, and ACTB genes, the…

Our cohort comprises 34 families in which a presumably autosomal recessive disease defied molecular diagnosis by clinical exome sequencing (short-read sequencing-based) and reanalysis performed on the index individual for each family (Fig. 1). The index patient in each family was subjected to an average of 10 × depth lrWGS except for Family F8602…

Colorectal cancer (CRC) is a malignant tumor with high morbidity and mortality. It is well-accepted that dysregulated lncRNAs are closely related to the development of CRC. In this study, the function and mechanism of RNASEH1-AS1 in CRC were investigated. RT-qPCR and western blot detected the expression of targeted genes in…

Background: Colorectal cancer (CRC) is one of the most common malignancies and is characterized by reprogrammed metabolism. Ferroptosis, a programmed cell death dependent on iron, has emerged as a promising strategy for CRC treatment. Although small nucleolar RNAs are extensively involved in carcinogenesis, it is unclear if they regulate ferroptosis…

Cross-posted on biostars: www.biostars.org/p/9581891/ I was wondering if make sense to perform an Enrichment Analysis from results obtained by RT-qPCR. I know you tipically do this with some NGS results, but I have 50 genes done by RT-qPCR I would be looking for pathways or disease association in an standardized…

Forum:Feasibility of Enrichment Analysis from RTqPCR results 0 I was wondering if I can perform an Enrichment Analysis from results obtained by RT-qPCR. I know you tipically do this with some sequenced results, but I have 50 genes done by RT-qPCR Thanks in advance GO RT-qPCR Enrichment-Analysis • 50 views…

MicroRNAs are vital gene expression regulators, extensively studied worldwide. The large-scale characterization of miRNAomes is possible using next-generation sequencing (NGS). This technology offers great opportunities, but these cannot be fully exploited without proper and comprehensive bioinformatics analysis. This may be achieved by the use of reliable dedicated software; however, different…

Reagents miRNeasy Serum/Plasma Advanced Kit (Qiagen, Hilden, Germany, # 217204); TaqMan® Advanced miRNA cDNA Synthesis Kit (Applied Biosystems, Bedford, MA, USA, #A28007); TaqMan® Fast Advanced Master Mix (Applied Biosystems, Bedford, MA, USA, # 4444556); TaqMan® Advanced miRNA Assays Single-tube assays (Applied Biosystems, Bedford, MA, USA, # A25576: 478293_mir, Spike-In cel-miR-39-3p;…

With all COVID-19 restrictions being lifted in Norway, and no obligation for mask use, not even in healthcare facilities, the present study aimed to assess the presence of SARS-CoV-2 in air samples from various indoor and outdoor spaces, including both healthcare and non-healthcare related facilities. We found that, out of…

Doxo and Abe promote cellular senescence accompanied by an altered profile of senescence-related genes in breast cancer cells Doxo and Abe were used to treat breast cancer cells (human triple-negative breast cancer cell line MDA-MB-231 and human luminal A subtype breast cancer cell line MCF-7) for 24 h, without a robust…

The development of chemotherapy resistance is a major obstacle for cervical cancer (CC) patients. Exosome-mediated transfer of circular RNAs (circRNAs) was found to have relevance to the CC. This study is designed to explore the role and mechanism of exosomal circRNA synaptotagmin 15 (circSYT15) on cisplatin (DDP) resistance in CC….

Materials The Cell Counting Kit-8 (CCK-8) was ordered from Yeasen BioTechnologies co, Ltd. (Shanghai, China). Anti-GAPDH was purchased from Proteintech Group, Inc. (Chicago, USA). Anti RhoA was purchased from Novus Biologicals (Colorado, USA). Antiserum against PRV glycoprotein gB was generated by immunization of mice with purified recombinant gB. Goat anti-Mouse…

InsightAce Analytic Pvt. Ltd. has announced the publication of a market research report titled “Global mRNA Extraction and Purification Market– By Product (Kits/Reagents, Instruments) End-user (Academics and Research Institutes, Pharmaceutical & Biotechnology Companies, Contract Research Organizations, Other End Users) Trends, Industry Competition Analysis, Revenue and Forecast To 2030.” According to…

Clinical samples Thirty nasopharyngeal aspirates were collected from individuals with symptoms of respiratory tract infections, and tested positive for HRV-A (5 clinical samples), HRV-B (20 clinical samples), or HRV-C-positive (5 clinical samples) based on the method, as previously described [27]. Ten other specimens were tested negative for HRV-B, including human…

Transcription and RNA processing The occupancy \({n}_{{{{{{\rm{ps}}}}}}}\) of the TSS of the active rDNA by Pol I follows the kinetic equation $$\frac{d}{{dt}}{n}_{{{{{{\rm{ps}}}}}}}={k}_{{{{{{\rm{on}}}}}}}\rho \left(1-{n}_{{{{{{\rm{ps}}}}}}}\right)-{k}_{{{{{{\rm{off}}}}}}}{n}_{{{{{{\rm{ps}}}}}}}-{k}_{{{{{{\rm{e}}}}}}}{n}_{{{{{{\rm{ps}}}}}}}.$$ (5) Equation (5) suggests that the occupancy \({n}_{{{{{{\rm{ps}}}}}}}\) changes due to the binding of Pol I from the FC (the first term), the unbinding of Pol I…

CFAV The CFAV used in this study was derived from Ae. aegypti Aag2 cells cultured in Schneider’s Drosophila Medium (Gibco, Paisley, UK) supplemented with 10% fetal bovine serum (Gibco, Thornton, Australia). The Aag2 cells were disrupted by three cycles of freeze-thawing and the supernatant was harvested as the CFAV stock…

Differential abundance analysis of urinary RNAs in CKD patients compared to healthy controls To explore the abundance of urinary RNAs, we performed an analysis using a dataset consisting of 80 CKD samples (GSE121978) and 47 healthy controls (GSE128359). This analysis encompassed a wide range of RNA types, including 36 circRNAs,…

Kitted assays for clinical laboratories streamline the detection of challenging genes and variants to improve results for underrepresented populations MINNEAPOLIS , Nov. 2, 2023 /PRNewswire/ — Bio-Techne Corporation (NASDAQ:TECH) today announced that Asuragen, a Bio-Techne brand, will present new data on clinical laboratory testing solutions related to cystic fibrosis variant detection, cancer…

Phenotypic response of two pineapple varieties to cold stress Notable differences in flowering time were observed for the two genotypes used in this study. The first signs of flowering were observed up to two weeks later in Dole-17 (precocious flowering tolerant) than in MD2 (precocious flowering susceptible) (Supplementary Figure S1)….

RT-qPCR primer specificity can be measured using a bioinformatics workflow that involves analyzing potential mismatches, cross-matches, co-amplification of multiple gene splice variants, and sub-optimal amplicon sizes in silico. This workflow utilizes publicly available resources such as NCBI Primer BLAST, in silico PCR in UCSC genome browser, and Ensembl DNA database…

Animals All mice were housed in the AALAC-accredited Animal Resource Center at Montana State University. All animal use protocols were approved by the Montana State University Institutional Animal Care and Use Committee (IACUC) (protocol No. 2020-15-IA; Bozeman, MT). The study fulfilled the ARRIVE guidelines, and all experiments complied with relevant…

1China Animal Health and Epidemiology Center, China 2College of Life Sciences and Food Engineering, Hebei University of Engineering, China The final, formatted version of the article will be published soon. Notify me Receive an email when it is updated You just subscribed…

Introduction Arteriosclerosis obliterans (ASO) is a chronic disorder with atherosclerosis involving lower extremity arteries leading to arterial stenosis or occlusion. The incidence continues to increase with age, affecting about 202 million people worldwide to varying degree.1–3 Despite the proposed theories of lipid infiltration, arterial intimal injury, and chronic inflammation, the…

In a recent study published in the journal Nature, researchers identify the mechanisms through which double-stranded break (DSB)-induced compartment formation orchestrates the deoxyribonucleic acid (DNA) damage response (DDR). Study: Chromatin compartmentalization regulates the response to DNA damage. Image Credit: CI Photos / Shutterstock.com Background DNA DSBs are extremely hazardous lesions that could…

Upon sample return to Earth, next-generation sequencing is performed to identify genome-wide transcriptional defense responses. In addition, next-generation sequencing is used to examine the transcriptional response to spaceflight in immune-deficient tomatoes. Total RNA is extracted from each sample using TRIZOL. At approximately three-weeks post germination, RNA yields of more than…

Transcribed image text: Fig. 3. GIGVF2/AEHP complex formation is critical for repression of 1/nbf mRNA translation and enabling viral replication. (A) ELSA measurement of ifN-1) production in WT, 4EHP.KO, and GIGVF2-KO HEK 93 cels transiently expressing TLR3 folowing 6 h of treatment with 1 pgimL poly(:C. Data are presented KO…

Sponsored Content by MaxCyte, Inc.Oct 19 2023Reviewed by Maria Osipova Oligonucleotide drugs are an emerging area of development that hold great promise for personalized treatment of rare diseases. There are approximately 8,000 distinct rare diseases, each individually affecting fewer than one in 2,000 people. Collectively, rare diseases impact more than…

Tissue samples All the enrolled patients provided written informed consent. Patients who had undergone chemotherapy or radiotherapy before surgery were excluded from the study. Surgical procedures were performed to obtain 40 pairs of HCC tumor and adjacent non-tumor tissue specimens from patients admitted to the First Affiliated Hospital of Chengdu…

Objective: The etiopathogenesis of diabetes nephropathy (DN) has not yet been fully clarified. Finding effective treatments to prevent renal failure in DN patients has become the main focus of research in recent years. Circular RNA (circRNA) has been shown to play a momentous role in DN progression. Based on this,…

Study approval and patient consent The study protocol was approved by the Ethics Committee of Qinghai Provincial People’s Hospital (approval number: 2023-141) and conducted in accordance with the ethical principles for medical research involving human subjects described in the Declaration of Helsinki in addition to relevant Chinese laws and institutional…

The classification, structure, abundance and reproducibility of coronavirus DVGs RNA transcripts other than those encoded by the coronavirus genome are also synthesized during infection [27]. Based on whether they are relevant to transcription regulatory sequence (TRS) (Figure S1A), a sequence motif from which subgenomic mRNAs (sgmRNAs) are synthesized, coronavirus RNA…

Introduction Breast cancer (BC) is a malignant tumor originating from the epithelial tissue of the breast and is the most common malignancy in women.1 It is estimated that by 2040, there will be 3,000,000 new cases of BC and 100,000 deaths worldwide. Triple-negative breast cancer (TNBC), which accounts for 15–20%…

Heggie, T. W. Swimming with death: Naegleria fowleri infections in recreational waters. Travel Med. Infect. Dis. 8, 201–206 (2010). Article  PubMed  Google Scholar  Grace, E., Asbill, S. & Virga, K. Naegleria fowleri: Pathogenesis, diagnosis, and treatment options. Antimicrob. Agents Chemother. 59, 6677–6681 (2015). Article  CAS  PubMed  PubMed Central  Google Scholar …

A thermostable type I-B CRISPR-Cas system from P. thermoglucosidasius Geobacillus and Parageobacillus are ubiquitous in a wide range of environments, growing at temperatures ranging from 35–80 °C29. To survey the distribution of thermostable type I CRISPR-Cas systems in these genera, we extracted all available genomes from CRISPRCasFinder database30, and identified Type…

Circular RNAs (circRNAs) are promising biomarkers and therapeutic targets for acute kidney injury (AKI). In this study, we investigated the mechanism by which circRNA itchy E3 ubiquitin protein ligase (circ-ITCH) regulates sepsis-induced AKI. A sepsis-induced AKI mouse model was created using LPS induction and circ-ITCH overexpression. Circ-ITCH levels were confirmed…

Fungal infection To test a natural way of interaction between adult T. castaneum with B. bassiana conidia, insects were maintained feeding on conidia-covered corn kernels during different short time periods (i.e., 3, 6, 12, and 24h), and then separated and maintained in rearing conditions. At all time periods assayed, T….

Introduction There are several promising applications for human-induced pluripotent stem cells (IPSCs), including cell replacement therapies, tissue engineering, and high-throughput toxicological and pharmacological screening.1 The scalable expansion and differentiation of IPSCs are demanding and inefficient; in particular, they commonly require the use of two-dimensional (2D) cultures.2 Data on stem cell…

Selection of RT-RAA amplification primers and HIV-1 RNA nucleic acid target, development of ERASE detection method for HIV-1 RNA In this study, a rapid nucleic acid detection assay for HIV-1 RNA was developed based on the ERASE method for COVID-19 detection established by our collaborator (Fig. 1A). First, HIV-1 RNA extracted…

Circular RNAs are a novel class of RNA molecules that are covalently closed into a ring structure. They are an epigenetic regulatory mechanism, and their best-studied function is regulation of microRNA activity. As such circular RNAs may be involved in the switch from acute to chronic pain. They have previously…

Patient characteristics and PDX establishment Between February 2016 and July 2020, 787 pediatric, adolescent and young adult patients with recurrent or refractory malignancies were enrolled in the MAPPYACTS trial;2 756 (96%) patients and their parents consented to the optional ancillary study of preclinical model development (Fig. 1a). 744 patients had a…

PPTP-seq development and validation PPTP-seq uses plasmid to integrate each CRISPRi-based TF perturbation and each promoter activity reporter into one construct. Each plasmid contains a CRISPRi cassette that constitutively expresses a single guide RNA (sgRNA) to repress a specific TF in the genome19 and a promoter-reporter cassette to measure the…

PRESS RELEASE Published September 15, 2023 InsightAce Analytic Pvt. Ltd. has announced the publication of a market research report titled “Global mRNA Extraction and Purification Market– By Product (Kits/Reagents, Instruments) End-user (Academics and Research Institutes, Pharmaceutical & Biotechnology Companies, Contract Research Organizations, Other End Users) Trends, Industry Competition Analysis, Revenue…

Filtering mitochondrial genes in DESEq results 1 Hi all! I took bulk RNAseq data done on leukaemia cells into R and I carried out deseq. I have three questions: I am confused about the limits people place on differentially expressed genes (DEGs). So far, I have only filtered for the…

Technique for Quantifying the Effects of siRNA on Gene Expression One technique that can be used to quantify the effects of siRNA on gene expression is RNA sequencing (RNA-seq). RNA-seq is a high-throughput sequencing method that allows for the measurement of gene expression levels by quantifying the abundance of RNA…

OsMATL2 is abundantly expressed in pollen. A) Phylogenetic tree of Arabidopsis, rice pPLA family, and MTL/NLD/ZmPLA1 orthologs. Phylogenetic tree was constructed by the neighbor-joining method using MEGAX program. Numbers next to the branches indicate bootstrap value percentages from 1,000 replications. Percentages (%) indicate protein identity with OsMATL2. The scale bar…

Bacterial isolation from Antarctic soil Soil samples were collected from the Cape Burk area (1: 74° 45′ 19.6″ S, 136° 48′ 44.6″ W)51. The soil (1 g) was inoculated in a Reasoner’s 2A (R2A) liquid (composed of 0.5% proteose peptone, 0.05% casamino acid, 0.05% yeast extract, 0.05% dextrose, 0.05% soluble starch,…

ATXN3 mRNA For hit validation SK-N-SH wildtype cells were treated at effective concentrations of 10 µM for 2 h in 24 well plate format and triplicates. Total RNA was extracted with the RNeasy Mini kit (Qiagen). RT-qPCR reactions were performed with the QuantiTect SYBR Green RT-PCR Kit (Qiagen) in a 96 well…

doi: 10.1016/bs.acc.2023.03.002. Epub 2023 Mar 28. Affiliations Expand Affiliations 1 1st Department of Neurology, Medical School, Aeginition Hospital, National and Kapodistrian University of Athens, Athens, Greece. Electronic address: abougea@med.uoa.gr. 2 1st Department of Neurology, Medical School, Aeginition Hospital, National and Kapodistrian University of Athens, Athens, Greece. Item in Clipboard Anastasia…

Mice All animal husbandry and experiments were approved by the Caltech Institutional Animal Care and Use Committee (IACUC, protocol #1646). The EED mouse model was established following the previously published protocol9 with slight adaptations. On day 1 of the experiment, just-weaned, specific pathogen-free (SPF) C57BL/6 J male mice were received from…

A Ct strain severely inhibits plant growth in a nutrient-dependent manner A Ct strain, Ct61, isolated from a wild A. thaliana population in Spain, promotes plant growth under low Pi conditions by transferring phosphorus to the host3. In addition to Ct61, five different Ct strains have been isolated from various…

Study design This was an observational study conducted between October 2020 and June 2021 in five large metropolitan areas in Brazil, belonging to four of the five different Brazilian geographical regions (Recife, Belo Horizonte, São Paulo, Campo Grande and Curitiba). Dogs and cats whose owners tested positive for SARS-CoV-2 by…

1. Introduction Lung cancer is the most common cancer globally, with 2.21 million cases in 2020 and over 1.8 million deaths from lung cancer, making it the most common cause of cancer-related deaths [1]. In China, the estimated age-standardized incidence rate of lung cancer is as high as 34.8 per…

All animal procedures were reviewed and approved by the OHSU IACUC (eIACUC #B11243). All experiments were performed in accordance with AAALAC and ARRIVE guidelines. Age, sex, and N of the mice for each experiment can be found in Supplementary Data 1. Mouse creation Mice were created through Cyagen Biosciences (Santa Clara,…

Introduction Immune checkpoint inhibitors (ICIs) have revolutionized the treatment pattern of various cancers, including melanoma and non-small cell lung cancer (NSCLC).1–3 However, there are still a considerable proportion (40%–60%) of patients who do not respond or do not achieve durable responses to these drugs.4 Thus, it is urgent clinical need…

Du, W., Liu, G., Shi, N., Tang, D., Ferdek, P. E., Jakubowska, M. A., Liu, S., Zhu, X., Zhang, J., Yao, L., Sang, X., Zou, S., Liu, T., Mukherjee, R., Criddle, D. N., Zheng, X., Xia, Q., Berggren, P. O., Huang, W., … Fu, X. (2022). A microRNA checkpoint for…

Tissue Collection The study was approved by the Medical Ethics Committee at the Fuyang Traditional Chinese Medicine Orthopedics Hospital (2019-F027). OS tumor tissues and adjacent normal tissues were collected from 40 patients diagnosed with OS at the Fuyang Traditional Chinese Medicine Orthopedics Hospital from June 2018 to Dec 2019. Patient…

Overview of the sequencing information To explore the presence of circRNAs during spleen development, we assessed circRNAs expression in the spleen tissues of Meishan pigs at various developmental stage. We prepared and sequenced ribo-depleted total RNA-seq libraries, as shown in the flow chart (Fig. 1). Table S2 presents our rudimentary sequencing…

Today it is possible to obtain genome-wide transcriptome data from single cells using high-throughput sequencing (scRNASeq). The main advantage of scRNASeq is that the cellular resolution and the genome wide scope makes it possible to address issues that are intractable using other methods, e.g. bulk RNASeq or single-cell RT-qPCR. These…

Cells and culture conditions NRC CHO2353™ cell lines have been described previously16,17. CHO2353 is a clone derived from CHO-DXB11 cells which were selected based on its high production yields of recombinant proteins. A complete description of the origin and selection of this clone can be consulted in ref. 15. Clone…

Identification and functional analysis of 73 DEMRGs A total of 73 DEMRGs were identified between N0 samples and N1/N2/N3 samples of LSCC, including 59 up-regulated and 14 down-regulated genes in N0 samples relative to N1/N2/N3 samples (Fig. 1a, Supplementary Table 1). The expressions of DEMRGs were present in the heatmap (Fig. 1b)….

1. Introduction Diffuse large B-cell lymphoma (DLBCL) is an aggressive malignant lymphoid neoplasm, which accounts for 40% of all malignant lymphoid neoplasms and includes morphologically and molecularly heterogeneous disease subtypes [1,2]. According to gene expression profiling, different forms of DLBCL are subdivided into germinal center B-cell-like (GCB-DLBCL) and activated B-cell-like…

NEW YORK – A team of researchers from the California Institute of Technology has developed a new nanoparticle transport system that can achieve programmable RNA export from living mammalian cells. Described in a Cell paper published last month, the mechanism, termed controlled output and uptake of RNA for interrogation, expression,…

Overview of the study design. We conducted RT-qPCR on 670 plasma samples, followed by metagenomic sequencing of 593 of the samples, received from (i) individuals suspected to have Lassa Fever (LF; caused by Lassa virus, LASV), collected from teaching hospitals with clinical expertise in viral hemorrhagic fevers; (ii) suspected infectious…

Allergy, Immunity and Respiratory Location:  Southampton General Hospital Salary:   £34,980 per annum Full Time Fixed Term for 29 months Closing Date:  Friday 18 August 2023 Interview Date:   To be confirmed Reference:  2405823FC We are seeking to recruit a Postdoctoral Research Fellow in the Faculty of Medicine to work…

To date, there have been reports of three balanced translocations and one microdeletion involving 12q24 associated with hypogonadism or KS1,2,3,4. In a case reported in 1983, all three brothers of Vietnamese Chinese origin were found to have severe primary hypogonadism with a karyotype of 46,XY,t(1;12)(p32;q24)3. Another case, published in 1990,…

Introduction Cutaneous melanoma is a kind of highly aggressive and metastatic cancer.1 It only occurs in 5% of all skin cancers but causes more than 75% of deaths.2,3 Melanoma can be cured by surgical removal at an early stage.4 However, when the disease progresses to an advanced stage (stage IV),…

1Sun Yat-sen University, China 2Sun Yat-sen Memorial Hospital, China 3Henan University of Science and Technology, China The final, formatted version of the article will be published soon. Notify me Receive an email when it is updated You just subscribed to…

M6A regulators are likely to have a significant effect on HBV-related HCC Twenty m6A regulators, including 12 readers, 7 writers, and 1 eraser, were identified in the TCGA and GEO cohorts. The correlation network provided interactive information among the m6A regulators. The ratio of somatic mutations and CNV for the…

Giwa, A. et al. Techno-economic assessment of the sustainability of an integrated biorefinery from microalgae and Jatropha: A review and case study. Renew. Sustain. Energy Rev. 88, 239–257 (2018). Article  CAS  Google Scholar  Piloto-Rodríguez, R. et al. An approach to the use of Jatropha curcas by-products as energy source in…

NEW YORK – Researchers looking for circular RNA (circRNA) biomarkers in RNA-seq datasets should consider using an ensemble of bioinformatics tools, according to a recent benchmarking study. Led by researchers from Belgium’s Ghent University, an international coalition applied 16 different software-based circRNA identification tools to the same RNA-seq dataset generated…

Boger B, Fachi MM, Vilhena RO, Cobre AF, Tonin FS, Pontarolo R. Systematic review with meta-analysis of the accuracy of diagnostic tests for COVID-19. Am J Infect Control. 2021;49(1):21–9. doi.org/10.1016/j.ajic.2020.07.011. Article  CAS  PubMed  Google Scholar  Sule WF, Oluwayelu DO. Real-time RT-PCR for COVID-19 diagnosis: challenges and prospects. Pan Afr Med…

作     者：Chen, Zhi Wu, Chenshuo Yuan, Yuxuan Xie, Zhongjian Li, Tianzhong Huang, Hao Li, Shuang Deng, Jiefeng Lin, Huiling Shi, Zhe Li, Chaozhou Hao, Yabin Tang, Yuxuan You, Yuehua Al-Hartomy, Omar A. Wageh, Swelm Al-Sehemi, Abdullah G. Lu, Ruitao Zhang, Ling Lin, Xuechun He, Yaqing Zhao, Guojun Li, Defa Zhang, Han  作者机构：Guangzhou Med Univ Qingyuan Peoples Hosp Affiliated Hosp 6 Qingyuan 511518 Guangdong Peoples R China Shenzhen Univ Inst Microscale Optoelect Coll Phys & Optoelect…

C1A protease family genes in the pineapple MD2 v2 genome Presence of either the C1 peptidase or I29 inhibitor domains were used as a signature to identify genes belonging to the C1A protease gene family9. 71 C1A genes were identified (AcC1A1–AcC1A71), and were distributed across 17 pineapple chromosomes (Fig. 1,…

Strain dynamics of the gut-associated microbiota within the first 24 months of life Shotgun metagenomics sequencing approaches were applied to the microbiomes of 11 healthy, vaginally delivered, full-term (>37 weeks of gestation) newborns, which were longitudinally sampled at 1-, 6-, 12-, and 24-months following birth (Figs. S1, S2, Supplementary Data 1). Consistent with…

Objective: To investigate the pathogenesis of three novel de novo CACNA1C variants (p.E411D, p.V622G, and p.A272V) in causing neurodevelopmental disorders and arrhythmia. Methods: Several molecular experiments were carried out on transfected human embryonic kidney 293 (HEK 293) and Chinese hamster ovary (CHO) cells to explore the effects of p.E411D, p.V622G,…

By sequencing samples derived from NAAT NP swabs and samples derived from positive BinaxNOW COVID-19 Ag Card swabs and comparing the proportions of successful sequencing, genome coverage, and RT-qPCR Ct values, we demonstrate that extraction reagents and sample processing do not significantly impact the ability to recover SARS-CoV-2 viral genomes….

Bacteria strains, plasmids, and growth conditions Enterococcus faecalis strains OG1RF and V583 are grown in tryptic soy broth (TSB) or plated on tryptic soy agar under aerobic conditions at 37 °C. All mutant strains are derivatives of OG1RF. The rlmN knockout in OG1RF (ΔrlmN) was generated by an in-frame deletion of…

doi: 10.1007/s10238-023-01127-9. Online ahead of print. Affiliations Expand Affiliations 1 Department of Infectious Disease, the Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, People’s Republic of China. 2 Department of Hematology, the Second Affiliated Hospital of Dalian Medical University, Dalian, 116023, Liaoning, People’s Republic of China. 3 Department of…

(A) Volcano plot of log2FC of RNA levels (FXS vs TD). Statistically significant changes (P value <0.0002) are shown as blue dots (down-regulated) and red dots (up-regulated). Gray dots refer to unchanged RNAs. (See also Dataset S2). (B) Histograms for TPM values for RNAs that are up or downregulated in…

Global identification of 3′ ends Initial computational predictions of open reading frames (ORFs) in B. burgdorferi38 overlooked elements such as mRNA boundaries, sRNA genes, and translation initiation from non-canonical start codons. B. burgdorferi transcription start sites (TSSs) and 5′ processed RNA ends were previously detected using 5′RNA-seq37. Here we sought…

Somatic loss of LRIF1 or SMCHD1 in control myocytes leads to insufficient DUX4 derepression We have previously shown that SMCHD1 and LRIF1L aid in transcriptional repression of the D4Z4 repeat as short-term depletion of either LRIF1L or SMCHD1 in muscle cells having a D4Z4 repeat of <20 units on a…

Ethics statement C57BL6, Rag1−/− (002216), Tcra−/− (002116), CCR5−/− (005427), Slc17a7-IRES2-Cre-D knock-in mice (Vglut1-cre, 023527), Cd4-cre (022071), and floxed EYFP (7903) mice purchased from The Jackson Laboratory were used for the current work. Mice were group-housed with two to five in an individual cage and kept under specific pathogen-free conditions with…

Identification and expression of GS genes in CHO cells Database analysis (see Supplementary information 1 indicated that the endogenous GS genes in CHO-K1 include two genes on chromosome 5 (GS5) and chromosome 1 (GS1), for which in-house RNAseq data and previous publication, listed in Supplementary Table S1, showed high and low…

1. Introduction Soybean (Glycine max (Linn.) Merr.) originated from China and is one of the most important legume and oil crops in the world [1]. The index evaluation of soybean oil is mainly related to the content and composition ratios of five fatty acids: oleic acid (C18:1), linoleic acid (C18:2),…

Background: Skin cutaneous melanoma (SKCM) is the deadliest dermatology tumor. Ongoing researches have confirmed that the NOD-like receptors (NLRs) family are crucial in driving carcinogenesis. However, the function of NLRs signaling pathway-related genes in SKCM remains unclear.Objective: To establish and identify an NLRs-related prognostic signature and to explore its predictive…

PRESS RELEASE Published June 27, 2023 mRNA Extraction and Purification Market to Record an Exponential CAGR by 2030 – Exclusive Report by InsightAce Analytic InsightAce Analytic Pvt. Ltd. has announced the publication of a market research report titled “Global mRNA Extraction and Purification Market– By Product (Kits/Reagents, Instruments) End-user (Academics…

Introduction Skin cutaneous melanoma (SKCM) is the most severe dermatologic malignancy, and its incidence has increased worldwide in recent years.1 SKCM accounts for 1% of all skin cancer patients, yet it is responsible for roughly 80% of all skin cancer deaths.2 Early-stage SKCM (localized or regional) can be surgically removed,…

Degenerative disk disease (DDD) that aggravates structural deterioration of intervertebral disks (IVDs) can be accompanied by painful inflammation and immunopathological progressions. Current surgical or pharmacological therapies cannot repair the structure and function of IVDs. Nucleus pulposus (NP) cells are crucial for the preservation or restoration of IVDs by balancing the…

Mitochondrial dysfunction and oxidative stress in neurodegenerative diseases. sFluorescence images of Calcein AM/PI staining in the CsA- or DMSO-treated NPCs. NPC pyroptosis mechanism via mtDNA mediated TLR9-NF-B-NLRP3 axis activation is unclear. In addition, we found that TLR9 recognized and bound mtDNA, which is critical for NF-B and the NLRP3 inflammasome…

Problem: The cyclic GMP-AMP synthase (cGAS)/Stimulator of Interferon Genes (STING) pathway is implicated in the development and progression of a myriad of inflammatory diseases including nonalcoholic steatohepatitis, colitis, sepsis, age-related macular degeneration, and cellular senescence. Thus, STING pathway inhibitors (in isolation or in combination with other therapies) could have wide…

NUP98 and RAE1 are enriched in progenitors and constitute a distinct complex Between the progenitor-state versus the differentiation state keratinocytes, we identified that 4 out of 5 nuclear-basket NUPs are significantly downregulated in differentiation (Fig. 1a), leveraging the RNA-seq data that we recently generated20. To determine how the enrichment of these…

doi: 10.1002/advs.202301766. Online ahead of print. Affiliations Expand Affiliations 1 Center for Systems Biology, Massachusetts General Hospital, Boston, MA, 02114, USA. 2 Cancer Center, Massachusetts General Hospital, Boston, MA, 02114, USA. 3 Department of Radiology, Massachusetts General Hospital, Boston, MA, 02114, USA. Item in Clipboard Jae-Sang Hong et al. Adv Sci…

The role of CD44 in tumorigenesis and as a prognostic marker in BLCA In this study, we determined CD44 mRNA expression in normal and in tumor tissues for BLCA via the use of the TIMER database (Fig. 1A). Our analysis found that there was a significant difference in the expression of…

1. Introduction With the global temperature rise, the dairy industry is confronted with a significant challenge brought about by severely damaging impacts of heat stress. Heat stress increases mortality rates and threatens the health of livestock, especially in highly productive and intolerant species. The increase in ambient temperature–humidity index (THI)…

DOI: 10.18129/B9.bioc.ReadqPCR     This package is for version 3.13 of Bioconductor; for the stable, up-to-date release version, see ReadqPCR. Read qPCR data Bioconductor version: 3.13 The package provides functions to read raw RT-qPCR data of different platforms. Author: James Perkins, Matthias Kohl, Nor Izayu Abdul Rahman Maintainer: James Perkins…