Tag: Salmon

Phylogenomics and species delimitation of the economically important Black Basses (Micropterus)

Arlinghaus, R. et al. Governing the recreational dimension of global fisheries. Proc. Nat. Acad. Sci. USA 116, 5209–5213 (2019). CAS  PubMed  PubMed Central  Article  Google Scholar  Cowx, I. G. & Gerdeaux, D. The effects of fisheries management practises on freshwater ecosystems. Fish. Manag. Ecol. 11, 145–151 (2004). Article  Google Scholar …

Continue Reading Phylogenomics and species delimitation of the economically important Black Basses (Micropterus)

New gene-edited tomatoes boost vitamin D levels

An international team of scientists primarily based out of the John Innes Centre in the United Kingdom has used gene-editing to create a vitamin D-enriched tomato. Using CRISPR-Cas9, they switched off an enzyme in tomatoes that ordinarily prevents the molecular forerunner of vitamin D3 (called 7-dehydrocholesterol, or 7-DHC) from accumulating…

Continue Reading New gene-edited tomatoes boost vitamin D levels

biopython – Pipeline for paired end RNA sequence data to proteins

This is a much more complicated question than it might seem. First, you need to understand how RNA-Seq works, and what your data really is. Your “paired-end files” contain reads, which will contain fragments of transcripts. Since those are only fragments of transcripts, you don’t know what frame they are…

Continue Reading biopython – Pipeline for paired end RNA sequence data to proteins

Can Differential Isoform expression analysis can be performed using DESeq2 package

Can Differential Isoform expression analysis can be performed using DESeq2 package 0 @03ddb485 Last seen 9 hours ago India Hello, I am want to perform differential isoform expression (DIE) analysis for RNAseq data from human. Can I use DESeq2 for this by inputting the transcript level abundance and getting differential…

Continue Reading Can Differential Isoform expression analysis can be performed using DESeq2 package

Validate RNAseq salmon quantification pipeline

Validate RNAseq salmon quantification pipeline 1 Hi, I’ve written a pipeline to perform quantification from RNAseq data with salmon. I’m trying to find a way to evaluate the quality of my results. I was thinking to run the pipeline on available public dataset and compare my output with another analysis….

Continue Reading Validate RNAseq salmon quantification pipeline

DESeq2 on allelic reads

DESeq2 on allelic reads 1 @ea088d93 Last seen 26 minutes ago Canada Hello, Can I use DESeq2 to perform differential gene expression on allelic reads? I have allelic reads quantified for each parental allele/copy I have 2 treatments (control vs ethanol-exposed) I want to perform differential gene expression to see…

Continue Reading DESeq2 on allelic reads

Separate exogenous from endogenous transcripts using Salmon RNAseq DTU

Dear friends, We are trying to use Salmon for DTU analysis. We want to separate exogenous from endogenous transcripts by following this post www.biostars.org/p/443701/ and this paper f1000research.com/articles/7-952 We are focusing on a gene called ASCL1 (endo-ASCL1). We transduced cells with lentiviral vector containing ASCL1 ORF only (Lenti-ASCL1). There should…

Continue Reading Separate exogenous from endogenous transcripts using Salmon RNAseq DTU

#1008368 – pigx-rnaseq: FTBFS: configure: error: R package rmarkdown not found.

#1008368 – pigx-rnaseq: FTBFS: configure: error: R package rmarkdown not found. – Debian Bug report logs Reported by: Lucas Nussbaum <lucas@debian.org> Date: Sat, 26 Mar 2022 21:12:04 UTC Severity: serious Tags: bookworm, ftbfs, sid Found in version pigx-rnaseq/0.0.19-2 Reply or subscribe to this bug. Toggle useless messages Report forwarded to…

Continue Reading #1008368 – pigx-rnaseq: FTBFS: configure: error: R package rmarkdown not found.

Using salmon in Galaxy

Hi everyone. I am executing Salmon in Galaxy in order to carry out gene quantification from mouse RNA-Seq data (6 samples). To do so, I am providing a reference genome (cDNA, in fasta format), the processed reads (in fastqsanger.gz format) of one of these samples (after executing Trim-Galore) and a…

Continue Reading Using salmon in Galaxy

Should I trim adapter sequences and filter by phred score, before alignment by salmon? : bioinformatics

First, trimming adapters is definitely necessary as they are essentially a form of contamination. For quality trimming and filtering I would highly recommend reading the following: Trimming of sequence reads alters RNA-Seq gene expression estimates Essentially they show that aggressive trimming is a problem. To quote from the Conclusions: The…

Continue Reading Should I trim adapter sequences and filter by phred score, before alignment by salmon? : bioinformatics

Low transcript quantification with Salmon using GRCm39 annotations

Hi everyone, first time working with mouse samples and unfortunately, there are fewer resources available for the latest mouse Ensembl genome than I was expecting. What I’ve done: I performed rRNA depletion on total RNA extracted from mouse tissue and created Illumina libraries using a cDNA synthesis kit with random…

Continue Reading Low transcript quantification with Salmon using GRCm39 annotations

A single-cell atlas of human and mouse white adipose tissue

Rosen, E. D. & Spiegelman, B. M. What we talk about when we talk about fat. Cell 156, 20–44 (2014). CAS  PubMed  PubMed Central  Google Scholar  Kahn, S. E., Hull, R. L. & Utzschneider, K. M. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature 444, 840–846 (2006)….

Continue Reading A single-cell atlas of human and mouse white adipose tissue

From scientific name to taxonomy information entrez

From scientific name to taxonomy information entrez 1 Hi all, I have a txt file with a list of scientific names of plants and I would like to obtain a final file with taxonomy information. For example, if one of my organism is Acalypha hispida, I would like to obtain…

Continue Reading From scientific name to taxonomy information entrez

Genomic variation from an extinct species is retained in the extant radiation following speciation reversal

Vamosi, J. C., Magallon, S., Mayrose, I., Otto, S. P. & Sauquet, H. Macroevolutionary patterns of flowering plant speciation and extinction. Annu. Rev. Plant Biol. 69, 685–706 (2018). CAS  PubMed  Google Scholar  Rhymer, J. M. & Simberloff, D. Extinction by hybridization and introgression. Annu. Rev. Ecol. Syst. 27, 83–109 (1996)….

Continue Reading Genomic variation from an extinct species is retained in the extant radiation following speciation reversal

UniProt: A0A1S3QEZ7_SALSA

ID A0A1S3QEZ7_SALSA Unreviewed; 104 AA. AC A0A1S3QEZ7; DT 12-APR-2017, integrated into UniProtKB/TrEMBL. DT 12-APR-2017, sequence version 1. DT 02-JUN-2021, entry version 11. DE SubName: Full=lipopolysaccharide-induced tumor necrosis factor-alpha factor homolog isoform X2 {ECO:0000313|RefSeq:XP_014038412.1}; GN Name=LOC106591717 {ECO:0000313|RefSeq:XP_014038412.1}; OS Salmo salar (Atlantic salmon). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; OC Actinopterygii;…

Continue Reading UniProt: A0A1S3QEZ7_SALSA

genepattern/Salmon.Indexer: Salmon indexer wrapper module for GenePattern.

GitHub – genepattern/Salmon.Indexer: Salmon indexer wrapper module for GenePattern. You can’t perform that action at this time. You signed in with another tab or window. Reload to refresh your session. You signed out in another tab or window. Reload to refresh your session. Read more here: Source link

Continue Reading genepattern/Salmon.Indexer: Salmon indexer wrapper module for GenePattern.

Time-course RNASeq of Camponotus floridanus forager and nurse ant brains indicate links between plasticity in the biological clock and behavioral division of labor | BMC Genomics

1. Sharma VK. Adaptive significance of circadian clocks. Chronobiol Int. 2003;20(6):901–19. PubMed  Google Scholar  2. Paranjpe DA, Sharma VK. Evolution of temporal order in living organisms. J Circadian Rhythms. 2005;3(1):7. PubMed  PubMed Central  Google Scholar  3. Yerushalmi S, Green RM. Evidence for the adaptive significance of circadian rhythms. Ecol Lett….

Continue Reading Time-course RNASeq of Camponotus floridanus forager and nurse ant brains indicate links between plasticity in the biological clock and behavioral division of labor | BMC Genomics

python – Missing input files after defining them in function

I am trying to do QC on RNAseq data that is tarballed. I am using Snakemake as a workflow manager and am aware that Snakemake does not like one-to-many rules. I defining a checkpoint would fix the problem but when I run the script I get this this error message…

Continue Reading python – Missing input files after defining them in function

RNA-seq analysis cloud server

RNA-seq analysis cloud server 1 Hi all, I have some RNA-seq of mice (around 200GB) and I want to perform a RNA-seq analysis (including QC, mapping, quantification, differential expression analysis). But I don’t know how to choose a server. Could anyone can tell me to process such a dataset, how…

Continue Reading RNA-seq analysis cloud server

The 2021 Good Tech Awards

In the tech industry, 2021 was a year of profits and pivots. Thanks in part to the pandemic and the digitization of our lives, all of the big tech companies got bigger. Facebook changed its name to Meta, Jeff Bezos went to space, Jack Dorsey left Twitter and Silicon Valley…

Continue Reading The 2021 Good Tech Awards

Help needed for Ensembl Gene ID conversion for RNA-seq data

Hello All, I am new to the RNA-seq world and especially new to the bioinformatics side. We recently completed a RNA-seq experiment (total RNAs) on human samples and we used illumina’s Dragen RNA pipeline which generated salmon gene count (.sf) output files. In the files, the gene ID is in…

Continue Reading Help needed for Ensembl Gene ID conversion for RNA-seq data

Tximport in usegalaxy

Tximport in usegalaxy 0 Devon Ryan: Please help in resolving this issue. How to use tximport in usegalaxy to convert transcript ID(DESEQ2-SALMON) to gene ID. I want to get gene ids from the results of deseq2(salmon) . Which GTF should be used for tximport. Iam getting the following error in…

Continue Reading Tximport in usegalaxy

deseq2 machine sizing best practices for very large data set

deseq2 machine sizing best practices for very large data set 0 @aa611017 Last seen 8 hours ago United States I want to perform differential expression analysis on a data set containing 17,000 samples. The salmon quant.sf files are about 1.5 Tb. based on my naive understanding of R and R…

Continue Reading deseq2 machine sizing best practices for very large data set

Quick questions about Alevin and AlevinQC

I have just started using Alevin and I have few basic queries. And I am asking this because I couldn’t find it in the documentation How to know how many reads are uniquely mapping and how many are multi mapping when doing quantification with Alevin. The alevin_meta_info.json says Number of…

Continue Reading Quick questions about Alevin and AlevinQC

Transposition and duplication of MADS-domain transcription factor genes in annual and perennial Arabis species modulates flowering

Annual and perennial species occur in many plant families. Annual plants and some perennials are monocarpic (flowering once in their life cycle), characterized by a massive flowering and typically produce many seeds before the whole plant senesces. By contrast, most perennials live for many years, show delayed reproduction, and are…

Continue Reading Transposition and duplication of MADS-domain transcription factor genes in annual and perennial Arabis species modulates flowering

Genome-wide analysis reveals associations between climate and regional patterns of adaptive divergence and dispersal in American pikas

Alexander DH, Novembre J, Lange K (2009) Fast model-based estimation of ancestry in unrelated individuals. Genome Res 19:1655–1664 CAS  PubMed  PubMed Central  Article  Google Scholar  Alexander DH, Shringarpure SS, Novembre J, Lange K (2015) Admixture 1.3 software manual. UCLA Hum Genet Softw Distrib, Los Angeles Google Scholar  Angert AL, Bontrager…

Continue Reading Genome-wide analysis reveals associations between climate and regional patterns of adaptive divergence and dispersal in American pikas

Transcripts mapping using Salmon

Transcripts mapping using Salmon 0 Hi all, We have recently conducted total RNA-seq (~200M reads) and mRNA-seq (~100M reads) in the same samples. Following the RNA-seq, we used Salmon selective alignment (SA) to align the reads to the Ensembl human transcriptome. This resulted in a comparable number of reads (~40M)…

Continue Reading Transcripts mapping using Salmon

Differential splicing/transcript usage/isoform analysis of iCell8 (SMART-seq v2) full transcriptome single cell data in conjunction with Seurat

Differential splicing/transcript usage/isoform analysis of iCell8 (SMART-seq v2) full transcriptome single cell data in conjunction with Seurat 0 Dear all, I am currently analyzing single-cell data gathered using the iCell8 platform (Takara) that captures full transcripts using the SMART-seq v2 chemistry. The official pipeline by Takara (CogentAP) uses STAR for…

Continue Reading Differential splicing/transcript usage/isoform analysis of iCell8 (SMART-seq v2) full transcriptome single cell data in conjunction with Seurat

geneiD-genetranscript annotations

Hello, Trying to generate a frame with 2 columns: transcript_id and gene_id, in LINUX (gtf from esembl) grep -P -o ‘ESNCAGd{11} Equus_caballus.EquCab3.0.104.gtf’ > ensecag.txt grep -P -o ‘ESNCATd{11} Equus_caballus.EquCab3.0.104.gtf’ > ensecat.txt wc -l enseca* # To see if both files have the same length They are not the same length:…

Continue Reading geneiD-genetranscript annotations

Transcriptional noise detection and Salmon TPMs

Transcriptional noise detection and Salmon TPMs 1 Hello, I’m analysing RNA-seq data from two datasets (from healthy samples) and created a unique GTF file to identify new isoforms by using StringTie. Then I used Salmon to estimate their TPMs, but I have some questions hoping anyone can help me: 1)…

Continue Reading Transcriptional noise detection and Salmon TPMs

Bioconductor Forum

James W. MacDonald 57k 1 week, 5 days ago United States Answer: Biomart’s getBM returns no genes for an existing GO-term in grch38, and less the Michael Love 33k 1 week, 6 days ago United States Answer: Normalizing 5′ Nascent RNA-seq data to identify differentially expressed transcr Kevin Blighe 3.3k 2 weeks, 2 days ago Republic…

Continue Reading Bioconductor Forum

When importing my quant.sf files into R using tximport, should I set ‘ignoreTxVersion’ to True or False?

Hello, I’m working through my first batch of RNA-Seq analysis and unfortunately I don’t have an experienced bioinformatician to work with. My question is regarding tximport of my quant.sf files into R. I have been working with the EquCab3.0 reference transcriptome from NCBI to generate these quant.sf files, but I…

Continue Reading When importing my quant.sf files into R using tximport, should I set ‘ignoreTxVersion’ to True or False?

How do I match my transcript ID’s from NCBI to the corresponding gene ID’s to enable tximport into R?

How do I match my transcript ID’s from NCBI to the corresponding gene ID’s to enable tximport into R? 2 Hi all, New to RNA-Seq analysis and I tried to find an answer to this elsewhere. I have performed salmon alignment on my pair-end fastq files which has generated the…

Continue Reading How do I match my transcript ID’s from NCBI to the corresponding gene ID’s to enable tximport into R?

Senior Programmer Analyst/Bioinformatics in New York, NY for Columbia University

  Job Seekers, Welcome to HERC Jobs Senior Programmer Analyst/Bioinformatics Columbia University Columbia University Job Type: Officer of Administration Bargaining Unit: Regular/Temporary: Regular End Date if Temporary: Hours Per Week: 35 Salary Range: Commensurate with experience  …

Continue Reading Senior Programmer Analyst/Bioinformatics in New York, NY for Columbia University

Problems with fragment length distribution output with Salmon

Problems with fragment length distribution output with Salmon 1 Hi all, New to RNA-Seq and I’m struggling with my Salmon alignment output. I tried to find an answer to this question on older posts but I couldn’t locate any other discussions, so apologies in advance if this has been covered…

Continue Reading Problems with fragment length distribution output with Salmon