Tag: sample

Unexprected Ensembl-vep results

Unexprected Ensembl-vep results 0 Hi.I got a VCF from an individual that shows symptoms of a known disease with known mutations. I run it with Ensembl-vep, expecting to find some of those mutations in the results, yet, all the consequences in the results are “intergenic-variant”.The command I used was: –cache…

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PCR duplicates in FFPE RNASeq

PCR duplicates in FFPE RNASeq 0 Dear all, I am working on 100 RNASeq data generated with a stranded protocol and a Novaseq run. I need to perform variant calling on these samples, however I am facing some problem. I have not access to DNA so exome/targeted amplification is not…

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Immune Infiltration and N(6)-Methyladenosine ncRNA Isoform Detection in Acute Lung Injury

Acute lung injury (ALI) is a severe form of sepsis that is associated with a high rate of morbidity and death in critically ill individuals. The emergence of ALI is the result of several factors at work. Case mortality rates might range from 40% to 70%. Researchers have discovered that…

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Common gene used to profile microbial communities — ScienceDaily

Part of a gene is better than none when identifying a species of microbe. But for Rice University computer scientists, part was not nearly enough in their pursuit of a program to identify all the species in a microbiome. Emu, their microbial community profiling software, effectively identifies bacterial species by…

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This startup wants you to have a personal stem cell stash

Salvatore Viscomi always wanted to be involved in a science fiction project — and he found one. Viscomi is the chief medical officer of GoodCell, a Massachusetts-based startup where people can bank their own white blood cells with a simple blood draw. Labs can then tweak those cells to become…

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Research opens the door to improved treatments for congenital heart disease

Researchers at Baylor College of Medicine, Texas Heart Institute, Texas Children’s Hospital and collaborating institutions have improved our understanding of the mechanisms underlying the progression of congenital heart disease (CHD) – a spectrum of heart defects that develop before birth and remain one of the leading causes of childhood death….

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Molecular Modeling Software for Chemistry Market Research With ChemDoodle, ChemAxon, Hypercube, ACD/ChemSketch, Avogadro

“ A2z Market Research announces the release of the report ‘ Molecular Modeling Software for Chemistry Market Research Report by Category, form, Product, Type, End-User, Region – Global Forecast to 2027. The primary drivers of this growth are the increasing need for market information and sustainability of key trends.’…

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COVID-19 mRNA vaccines linked to higher risk of side effects vs. traditional vaccines

Although the risk of adverse events (AEs) was increased for mRNA-based COVID-19 vaccines compared to traditional vaccines, the efficacy and benefits of mRNA vaccination outweighed the associated risks, according to a study in International Immunopharmacology. Researchers conducted a meta-analysis to evaluate the safety of several types of COVID-19 vaccines. They…

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Electroporation Generator (NanoKnife) market share to record robust growth through 2028

The Electroporation Generator (NanoKnife) market report entails a complete analysis of the past, present, and future business landscape with respect to important aspects like the key growth drivers, restraints, as well as the opportunities and associated risks. The authors of the report claim…

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Sr. Research Associate, Bioinformatics job with F. Hoffmann-La Roche AG

The Bioinformatics Senior Research Associate is responsible for designing and troubleshooting PCR-based assays for use with the GenMark Dx ePlex platform. The ideal candidate must be able to work in a collaborative research and IVD product development environment and demonstrate ability to analyze sequence data to drive assay development. The…

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Pooled shRNA Library Screening to Identify Factors that Modulate a Drug Resistance Phenotype

High-throughput RNA interference (RNAi) screening using a pool of lentiviral shRNAs can be a tool to detect therapeutically relevant synthetic lethal targets in malignancies. We provide a pooled shRNA screening approach to investigate the epigenetic effectors in acute myeloid leukemia (AML). The overall goal of the following video is to…

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Single-cell analyses define a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer

Mapping molecular changes across malignant transformation We generated single-cell data for 81 samples collected from eight FAP and seven non-FAP donors (Fig. 1a and Supplementary Tables 1 and 2). For each tissue, we performed matched scATAC-seq and snRNA-seq (10x Genomics). We obtained high-quality single-cell chromatin accessibility profiles for 447,829 cells…

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Transcriptomic and proteomic profiling of peptidase expression in Fasciola hepatica eggs developing at host’s body temperature

From the bovine liver, we isolated 97 live F. hepatica adults. After overnight cultivation, we recovered approx. 228,000 laid eggs, which we divided in three groups. The first group (T0) was immediately frozen at − 80 °C, while the other two groups (T5 and T10) were incubated for 5 and 10 days at…

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A highly sensitive fluorescent aptasensor for detection of prostate specific antigen based on the integration of a DNA structure and CRISPR-Cas12a

. 2022 Aug 1;1219:340031. doi: 10.1016/j.aca.2022.340031. Epub 2022 Jun 3. Affiliations Expand Affiliations 1 Targeted Drug Delivery Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran; Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran. 2 Pharmaceutical Research Center, Pharmaceutical Technology Institute,…

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YP5260 – YFull YTree Info

Sample ID Country / Language Info Ref File Testing company Statistics Status I7021 Mongolia (Bulgan) C-F15910 C-F15910*, C-Y507 Hg19 .BAM Ancient 3X, 20.2 Mbp, 40 bp NEO249 Russia (Chukotskiy avtonomnyy okrug) C-F15910* —— Hg19 .BAM Ancient 1X, 7.2 Mbp, 81 bp I11696 Mongolia (Bulgan) C-Y507 —— Hg19 .BAM Ancient 2X,…

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Molecular Dynamics Simulation Software Market by 2029

New Jersey (United States) – A2Z Market Research published new research on Global Molecular Dynamics Simulation Software covering the micro-level of analysis by competitors and key business segments (2022-2029). The Global Molecular Dynamics Simulation Software explores a comprehensive study on various segments like opportunities, size, development, innovation, sales, and overall…

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rprimer: an R/bioconductor package for design of degenerate oligos for sequence variable viruses | BMC Bioinformatics

Case study To illustrate and evaluate the functionality of rprimer, two assays targeted at norovirus GI were designed. Assay A: RT-qPCR for quantitative detection of norovirus GI The first task was to design a broadly reactive RT-qPCR assay for quantitative detection. For this, we decided to use the ‘ambiguous’ primer…

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08 compare visualization results of different annotation software

stay In the first two sections , We compared the differences vcf Use of annotation software , And convert the demerit recorded after the annotation into maf File format , because snpeff The comment result cannot be converted to maf, So we will compare later ANNOVAR、VEP、GATK Funcatator The results of…

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Bioimpendance Analysers Market Segmentation and Analysis Report, 2031

Wilmington, Delaware, United States: The demand in the global bioimpendance analysers market is surging owing to high growth potential amongst emerging economies such as Brazil and India. Rising private as well as public initiatives and investments aimed at supporting medical and healthcare research as well as increase in the domestic…

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Genome-wide association study of musical beat synchronization demonstrates high polygenicity

Savage, P. E., Brown, S., Sakai, E. & Currie, T. E. Statistical universals reveal the structures and functions of human music. Proc. Natl Acad. Sci. USA 112, 8987–8992 (2015). CAS  PubMed  PubMed Central  Article  Google Scholar  Ravignani, A., Delgado, T. & Kirby, S. Musical evolution in the lab exhibits rhythmic…

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How To Download Geo Data? Update New

Bioinformatics 101 | How to download RNA-Seq data from NCBI GEO | Bioinformatics for beginners Bioinformatics 101 | How to download RNA-Seq data from NCBI GEO | Bioinformatics for beginners Images related to the topicBioinformatics 101 | How to download RNA-Seq data from NCBI GEO | Bioinformatics for beginners Bioinformatics…

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A Generalized Method for Determining Free Soluble Phenolic Acid Composition and Antioxidant Capacity of Cereals and Legumes

Phenolic acids are important phytochemicals that are present in whole grains. They possess bioactive properties such as antioxidant protective functions. This work aimed at reporting on a generalized method for the HPLC identification, total phenolic content estimation, and determination of the antioxidant capacity of phenolic acids in cereals and legumes….

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CRISPR-Cas technology a new era in genomic engineering

Review doi: 10.1016/j.btre.2022.e00731. eCollection 2022 Jun. Affiliations Expand Affiliation 1 Department of Agriculture and Natural Resources, College of Agriculture, Science, and Technology, Delaware State University, Dover, DE 19901, United States of America. Free PMC article Item in Clipboard Review Ali Parsaeimehr et al. Biotechnol Rep (Amst). 2022. Free PMC article Show…

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Summary of Escherichia coli K-12 substr. MG1655, version 26.0

Summary of Escherichia coli, Strain K-12 substr. MG1655, version 26.0 Tier 1 Highly Curated Database Authors: Peter D. Karp1, Ingrid Keseler1, Carol Fulcher1, Anamika Kothari1, Suzanne Paley1, Markus Krummenacker1, Ian Paulsen2, Amanda Mackie2, Julio Collado-Vides3, Cesar Bonavides-Martinez3, Socorro Gama-Castro3, Alberto Santos-Zavaleta3, Victor H Tierrafria3 1SRI International, 2Macquarie University, 3Centro de…

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BY3 – YFull YTree Info

J-BY3 – YFull YTree Info SNPs currently defining J-BY3 BY3 / FGC15184     Sample ID Country / Language Info Ref File Testing company Statistics Status YF016315 —— J-FGC15174 J-FGC15174*, J-FGC15168*, J-FT258574 Hg38 .BAM FTDNA (Y500) 23X, 12.0 Mbp, 151 bp YF068400 Sudan (Janūb Kurdufān) J-FGC38453* —— Hg38 .BAM FTDNA (Y700)…

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Forage Bioinformatics Master’s student / AAFC

Molecular biology (e.g., DNA and RNA extraction). Rearing of plants in a greenhouse. Sample collection. Bioinformatics related to the assembly and annotation of a reference genome sequence. Work environment The candidate is required to be eligible or be enrolled as a Master’s student in the Department of Plant Sciences at…

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Electroporation Instruments Market 2022 to 2028: Market Value, Trend Analysis & Competition Tracking

“Electroporation Instruments Market Report, 2022 to 2028 Electroporation Instruments Market report provides deep exposure to Electroporation Instruments industry and main market trends. The market research report contains forecast market data, demand, price trends, and company shares of the leading Electroporation Instruments segmentation by geography. The report splits the market size,…

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python 3.x – PySpark on Jupyterhub K8s || Unable to query data || Class org.apache.hadoop.fs.s3a.S3AFileSystem not found

Pyspark Version: 2.4.5 Hive Version: 1.2 Hadoop Version: 2.7 AWS-SDK Jar: 1.7.4 Hadoop-AWS: 2.7.3 When I am trying to show data I am getting Class org.apache.hadoop.fs.s3a.S3AFileSystem not found while I am passing all the information which all are required. I passed all three for this config fs.s3.aws.credentials.provider org.apache.hadoop.fs.s3a.BasicAWSCredentialsProvider com.amazonaws.auth.InstanceProfileCredentialsProvider com.amazonaws.auth.EnvironmentVariableCredentialsProvider…

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python – ValueError: Unused step method arguments: {‘initvals’} in pymc3

I am trying to use Automated Recommendation Tool which utilizes pymc3 package but I am facing issues while executing the following code cell – %%time if run_art: art = RecommendationEngine(df, **art_params) else: with open(os.path.join(art_params[‘output_directory’], ‘art.pkl’), ‘rb’) as output: art = pickle.load(output) Following is the error that pops up – ValueError…

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Reporting results from fastp Read GC content

Reporting results from fastp Read GC content 1 Hi, I have some QC results for a Paired end seq before and after filtering (Mouse samples). I am unable to interpret the plot Read GC content and I am not sure if the results are fine in this case. I am…

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Metagenomics Sequencing Market Size 2022 Global Industry Revenue, Business Growth, Development Strategy, Trends, Demand and Applications Market Research Report Forecast 2025

Global “Metagenomics Sequencing Market” Research Report 2022 is a historical overview and in-depth study of the current & future market of the Metagenomics Sequencing industry. The report represents a basic overview of the Metagenomics Sequencing market share, and competitor segment with a basic introduction of key players, top regions with…

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exact counts tables for limma voom guide

exact counts tables for limma voom guide 1 Is there a way to download the exact counts table associated with the limma voom guide? specifically for the tutorial in chapter 15 of the limma voom guide (GSE64099). I would like to to run through the commands and recreate the plots…

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YP3952 – YFull YTree Info

Q-YP3952 – YFull YTree Info Sample ID Country / Language Info Ref File Testing company Statistics Status YF073154 Russia (Chechenskaya Respublika) / Chechen Q-YP3952* —— Hg38 .BAM FTDNA (Y700) 33X, 18.2 Mbp, 151 bp YF092378 Russia (Chechenskaya Respublika) / Chechen Q-BZ87 —— Hg38 .BAM FTDNA (Y700) 55X, 18.5 Mbp, 151…

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Bioconductor – sSNAPPY (development version)

DOI: 10.18129/B9.bioc.sSNAPPY     This is the development version of sSNAPPY; for the stable release version, see sSNAPPY. Single Sample directioNAl Pathway Perturbation analYsis Bioconductor version: Development (3.16) A single sample pathway pertrubation testing methods for RNA-seq data. The method propagate changes in gene expression down gene-set topologies to compute…

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PyMC3 Normal with variance per column

One can pass an argument for the shape parameter to achieve this. To demonstrate this, let’s make some fake data to pass as observed, where we use fixed values for epsilon that we can compare against the inferred ones. import numpy as np import pymc3 as pm import arviz as…

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Metagenomics – RPKM calculation

Normalization for comparing gene coverage values. RPKM corrects differences in both: sample sequencing depth and gene length. RPKM – Reads per kilo base per million mapped reads Formula RPKM = numReads / ( geneLength/1000 * totalNumReads/1,000,000 ) numReads – number of reads mapped to a gene sequence geneLength – length…

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Metagenomics Market Growth Drivers, Investment Opportunity and Product Developments 2032

insightSLICE has recently published the Global Metagenomics Market research report inclusive of authentic domain data. This market research report has been structured by seasoned analysts. The key research methodology used throughout this market report by insightSLICE research team is data triangulation which entails data mining, analysis of the impact of data variables on the market, and primary validation….

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Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

This protocol describes the extraction of volatile organic compounds from a biological sample with the vacuum-assisted sorbent extraction method, gas chromatography coupled with mass spectrometry using the Entech Sample Preparation Rail, and data analysis. It also describes culture of biological samples and stable isotope probing. This protocol allows us to…

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rna seq – R – [DESeq2] – How use TMM normalized counts (from EdgeR) in inputs for DESeq2?

I have several RNAseq samples, from different experimental conditions. After sequencing, and alignment to reference genome, I merged the raw counts to get a dataframe that looks like this: > df_merge T0 DJ21 DJ24 DJ29 DJ32 Rec2 Rec6 Rec9 G10 421 200 350 288 284 198 314 165 G1000 17208…

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What counts as a valid biological replicate in single cell RNAseq?

Forum:What counts as a valid biological replicate in single cell RNAseq? 3 I’ve run an experiment where I collected orans from 3x healthy control mice, and 3x post-injury mice – and ended up generating around 3000 individual single cells from each sample. For consistency and cost reasons, we pooled our…

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Roles of circRNAs | CMAR

Introduction Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, with an increasing global incidence, and a leading cause of cancer-related mortality.1 Although hepatitis B and C virus infections, cirrhosis, alcohol intake, and non-alcoholic fatty liver disease (NAFLD) are the main risk factors contributing to HCC.1,2 Approximately…

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RegEx: Listing all possibilities to build sample code in python

itertools.product() is the way to go here. If your proteins are all single characters then for each position in your “regex”, just put a string representing the valid proteins for that position into the itertools.product() arguments. For example [IG]…D.SG would become the following: p = ‘ABCDEFGHIJKLMNOPQRST’ # or whatever the…

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How do we predict on new unseen groups in a hierarchical model in PyMC3?

I’m just copying my answer from the pymc discourse thread if someone by chance runs into this question or another one like it here. First of all, beware of the centered hierarchical parametrization 1 you are using, it may lead to divergences and difficulties while fitting. That being said, your…

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Metagenomic Sequencing Market Size And Forecast

New Jersey, United States – The Verified Market Reports released the latest competent intelligence market research report on the Metagenomic Sequencing Market, The report aims to provide a thorough and accurate analysis of the Metagenomic Sequencing market, taking into account market forecast, competitive intelligence, technical risks, innovations, and other pertinent data. Its…

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Imaging Replicative Domains in Ultrastructurally Preserved Chromatin by Electron Tomography

This protocol presents a technique for high-resolution mapping of replication sites in structurally preserved chromatin in situ that employs a combination of pre-embedding EdU-streptavidin-Nanogold labeling and ChromEMT. We present a protocol for the electron microscopic visualization of the replicative domains in the cell by the EdU labeling of the newly…

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Binding of the HSF-1 DNA-binding domain to multimeric C. elegans consensus HSEs is guided by cooperative interactions

The heat-shock response is represented by a small set of genes in C. elegans We initially aimed at identifying those HSE-containing promoters that are most strongly upregulated under heat-stress conditions. Given that the HSR is complex in nematodes we used data from several heat-shock studies based on microarray and RNAseq…

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Antigen Detection Test Market See Huge Growth for New Normal

Antigen Detection Test Market research report is the new statistical data source added by Research Cognizance. “Antigen Detection Test Market is growing at a High CAGR during the forecast period 2022-2029. The increasing interest of the individuals in this industry is that the major reason for the expansion of this…

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Help with PCA plot from SNPRelate

Hello, BioStars community! I am working on a datase of 50 samples(between cases and controls) with genotyping data for ~200 SNPs. I ran SNPRelate PCA analysis without adding population data and tried to plot its results. My PCA plot seems a bit “strange” since the observations did not come together…

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Swarm immunology: harnessing blockchain technology and artificial intelligence in human immunology

Human immunology may soon benefit from the use of artificial intelligence and blockchain technologies. Here, we discuss how Swarm Learning could foster collaborative worldwide immunology studies that fully respect local data privacy regulations by sharing insights, not data. For decades, immunological research has benefited from highly standardized animal models. Yet,…

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Impact of sweet, umami, and bitter taste receptor (TAS1R and TAS2R) genomic and expression alterations in solid tumors on survival

Despite being best known for their role in taste sensing1, T2Rs and T1Rs have been identified in various extra-oral tissues where they serve diverse chemosensory roles17,18,19,20,21,22,48. Emerging data on the role of taste receptors in malignancy led us to explore the genetic and expression alterations for solid tumors and implications…

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Singular Genomics Partners with Qiagen to Enable Qiaseq Kits for the G4 Sequencing Platform

LA JOLLA, Calif., Feb. 28, 2022 (GLOBE NEWSWIRE) — Singular Genomics Systems, Inc. (Nasdaq: OMIC), a company leveraging novel next-generation sequencing (NGS) and multiomics technologies to empower researchers and clinicians, today announced a partnership with Agilent Technologies (www.agilent.com) to validate its NGS Target Enrichment Products, highly sensitive target enrichment panels…

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Computational Biology Software Market Future Scope including key players Insilico Biotechnology, Simulation Plus, Leadscope

  Latest research study from JCMR including most recent “Q1-2022” Global Computational Biology Software Market by Manufacturers, Regions, Type and Application, Forecast to 2022-2030. The Computational Biology Software Research report presents a complete assessment of the market and contains Future trend, Current Growth Factors, attentive opinions, facts, historical data, and statistically supported…

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Head of Instrumentation Team at European Molecular Biology Laboratory (EMBL)

About the team/job The European Molecular Biology Laboratory (EMBL) in Grenoble has an active research and international visitors program in structural molecular biology, making particular use of synchrotron X-rays and cryo-electron microscopy as structure determination techniques. EMBL Grenoble shares the European Photon and Neutron Science (EPN) Campus with the European…

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Animal Vaccines Market Size Worth $26.12 Billion By 2030, Due To Growing Incidence Of Food-Borne Zoonotic Diseases And Increasing Animal Husbandry | Grand View Research, Inc.

“Grand View Research, Inc. – Market Research And Consulting” According to a new report published by Grand View Research, The increasing outbreaks of cattle disease and the ever-expanding livestock population are the prime factors for the wide acceptance of the ruminants especially cattle vaccines globally. Moreover, rising pet ownership and…

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Computational Biology Software Market Worldwide Industry Share 2022-2028

“ The report studies the key segments in global Computational Biology Software industry, their growth in past few years, profiles and market sizes of individual segments, and gives a detailed overview of the profiles of various segments. The report also presents key products and various other products in the global…

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RPKM, how to normalize for library size

RPKM, how to normalize for library size 1 Hi everybody! I’m working on several metagenomes (so not trascriptomes), and I mapped high quality reads to a database made of nucleotide sequences through bowtie2. I want to convert raw mapping counts of each gene in RPKM. I know this method might…

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Restriction Endonucleases Products (REP) Market: Overview, Major Trends & Regional Assessment

Global Restriction Endonucleases Products (REP) Market: Overview Albany NY, United States: The leading applications for the products and solutions offered by the manufacturers and players functional in the landscape of the global restriction endonucleases products market include epigenetics, fragment length polymorphism or RFLP, cloning, sequencing, polymerase chain reaction, and restriction…

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Global Nucleic Acid Labeling Market 2022 to 2028 Latest Innovations and Major Players are Merck & Co., Inc., PerkinElmer, Inc.

The global markets are being disrupted by the arrival of industry 4.0, which is accompanied by digitalization, automation, and artificial intelligence. Fundamental geopolitical shifts are changing global economic power centers. As a result, The Brainy Insights has launched a research study on the future of the global Nucleic Acid Labeling…

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InteractiveComplexHeatmap on DESeq2 object with more than 2 groups

InteractiveComplexHeatmap on DESeq2 object with more than 2 groups 1 Hello all, I’m writing with the hope someone can clarify a doubt I have about the differential heatmap generated by the package InteractiveComplexHeatmap via the simple command interactivate(dds). I read the package documentation at bioconductor.org/packages/release/bioc/html/InteractiveComplexHeatmap.html, but I couldn’t find the…

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minimum number of protein sequences for a sequence logo

minimum number of protein sequences for a sequence logo 1 Hi, I’m interested in generating sequence logo of a series of defensin related proteins I’ve clustered using CD-Hit. There are aproximately 7600 sequences with about 2500 clusters, but many of them have few sequences per cluster. Which is the minimum…

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Open API Market Size, Trends and Forecast to 2029

New Jersey, United States – The Open API Market report includes the upcoming challenges and opportunities in the market. It ensures a strengthened position in the market and a growing product portfolio by providing all the important details related to the market growth. It reveals some of the key insights and focuses…

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losing large amount of reads when using Qiime2 vsearch joinpairs command

losing large amount of reads when using Qiime2 vsearch joinpairs command 0 Hi there! I am running various sediment samples (Illumina for amplicon 16S – V4V5 sequencing) through the Qiime2 pipeline, however when I use vsearch joinpairs to join my paired end reads I lose a bunch of reads. For…

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Genetic and chemotherapeutic influences on germline hypermutation

DNM filtering in 100,000 Genomes Project We analysed DNMs called in 13,949 parent–offspring trios from 12,609 families from the rare disease programme of the 100,000 Genomes Project. The rare disease cohort includes individuals with a wide array of diseases, including neurodevelopmental disorders, cardiovascular disorders, renal and urinary tract disorders, ophthalmological…

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Role of CD68 in tumor immunity and prognosis prediction in pan-cancer

Expression of CD68 in pan-cancer First, to fully clarify the expression of CD68 in pan-cancer, we matched the GTEx normal samples with TCGA tumor samples (Fig. 1A). We found that the levels of CD68 were significantly elevated (P < 0.01) in colon adenocarcinoma (COAD), glioblastoma multiforme (GBM), kidney renal clear cell carcinoma (KIRC),…

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Norgen 16S rRNA and ITS Library Preparation Kits

Analyze Bacterial and Fungal Microbiomes 16S rRNA Library Preparation Kits Simple and quick workflow. Library could be prepared in less than 4 hours Part of Norgen’s metagenomics workflow: collection, preservation, DNA isolation, sequencing, and analysis. Protocol optimized for DNA isolated from a large range of sample types Up to 384…

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ACADVL – PrimePCR Assay and Template | Life Science

Control assays and synthetic DNA templates were designed to facilitate the assessment of the key experimental factors impacting your real-time PCR results. DNA Contamination Control AssayUse the PrimePCR DNA contamination control assay to determine if genomic DNA (gDNA) is present in a sample at a level that may affect PCR…

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Z697 – YFull YTree Info

R-Z697 – YFull YTree Info SNPs currently defining R-Z697 Z697     Sample ID Country / Language Info Ref File Testing company Statistics Status YF009397 Sweden (Västra Götalands län) R-Z697* —— Hg19 .BAM FTDNA (Y500) 81X, 14.4 Mbp, 165 bp YF084333 Italy (Chieti) R-FT285492 —— Hg38 .BAM Dante Labs 14X, 23.4…

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Frontiers | Divergence With Gene Flow and Contrasting Population Size Blur the Species Boundary in Cycas Sect. Asiorientales, as Inferred From Morphology and RAD-Seq Data

Introduction Incipient species are critical for evolutionary biologists to study speciation, but they also challenge taxonomy due to gene flow or ancestral polymorphism. The former and contrasting population size lead to larger intraspecific than interspecific variations, a phenomenon called the species-definition anomaly zone (Jiao and Yang, 2021). The latter results…

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Our genetic strength in numbers

Although it’s 20 years since the first human genome was sequenced – that is, the entire collection of our DNA – our ability to ‘read’ our own instruction manual is no less astounding. We now know we have around three billion chemical base pairs that provide the code for approximately…

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Global CRISPR/Cas9 Market In- Depth Research, Industry Statistics 2022

Global “CRISPR/Cas9 Market” Reports 2022 provide key industry studies for CRISPR/Cas9 manufacturers with specific statistics, significance, definition, SWOT analysis, expert opinion and the latest developments in the world. The research report also covers market size, price, sales, revenue, market shares, gross margin, growth rate and cost structure. The report aims…

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Global Ultrasound Divices Market Size, Share, Trends and Future Growth Predictions till 2029

Global Ultrasound Divices Market with Coronavirus impact analysis based on product type, application, and region forecast till 2029. The report will help to understand the future market scenario and finding opportunities in terms of investment and profits. The outbreak of COVID-19 pandemic, the world economy has upended, and it is…

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New Products Posted to GenomeWeb: Thermo Fisher, Guardant Health, EmpowerDx, More

Thermo Fisher Invitrogen TrueTag Donor DNA Kits  Thermo Fisher Scientific has launched Invitrogen TrueTag Donor DNA kits for cell line engineering with CRISPR-Cas9 or TALEN technology. They allow researchers to enrich tagged cells and skip clonal isolation. The kits support four types of locus-specific knock-in templates and feature a one-step…

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Strange Per base sequence content of fastqc

Hi, all! I download fastq.gz files of GSE162708 from ENA which only have 2 files of each sample(usually scRNA-seq has 3 files I1 , R1 & R2 ). Then I run fastp as following Then I get QC report , but I can’t understand why Per base sequence content of…

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Plant Breeding and CRISPR Plants Market Trends 2022, Global Industry Share, Size, Global Industry Analysis, Key Market Growth Analysis, Segments, Emerging Technologies, Opportunity and Forecast 2027

The “Plant Breeding and CRISPR Plants Market” research report 2021-2027 offering the complete outline of market size, share and growth opportunities, industry segmentation by product type, applications and geographical representation. The report highlights product development along with technological upgrades that can boost the market growth. The Plant Breeding and CRISPR…

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nf-core presentation – HackMD

<!– .slide: data-background=”raw.githubusercontent.com/maxulysse/maxulysse.github.io/main/assets/img/svg/green_white_bg.svg&#8221; –> <a href=”nf-co.re”><img src=”raw.githubusercontent.com/nf-core/logos/master/nf-core-logos/nf-core-logo-darkbg.svg&#8221; width=”60%”></img></a> # What is nf-core\? [Maxime U Garcia](https://maxulysse.github.io/) ▸ [<i class=”fa fa-twitter” aria-hidden=”true”></i>@gau](https://twitter.com/gau/) | [<i class=”fa fa-github” aria-hidden=”true”></i>@maxulysse](https://github.com/maxulysse/) [Karolinska Institutet](https://ki.se/) | [Science for Life Laboratory](https://www.scilifelab.se/) Sweden — ## Overview – 🗄️ [Barntumörbanken](https://ki.se/forskning/barntumorbanken/) – 🧬 [NGI](https://ngisweden.scilifelab.se/) – 🍬 [Nextflow](https://www.nextflow.io/) – 🧙 [nf-core](https://nf-co.re/) — ##…

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Corporate Volunteering Platform Professional Market Records a Significant Growth by 2029

“ A comprehensive appraisal of the worldwide Corporate Volunteering Platform Professional market agglomerates basically engaged scientific outcomes and noteworthy data taking care of the vital necessities of the report peruses including market members across the worldwide Corporate Volunteering Platform Professional market, financial backers and business visionaries looking for profoundly conclusive…

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Identification of Potential Biomarkers for Progression and Prognosis of Bladder Cancer by Comprehensive Bioinformatics Analysis

This article was originally published here J Oncol. 2022 Apr 19;2022:1802706. doi: 10.1155/2022/1802706. eCollection 2022. ABSTRACT Background. Bladder cancer (BLCA) is a highly malignant tumor that develops in the urinary system. Identification of biomarkers in progression and prognosis is crucial for the treatment of BLCA. BLCA-related differentially expressed genes (DEGs)…

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Bioinformatics Software Professional Market Records a Significant Growth by 2029

“ A comprehensive appraisal of the worldwide Bioinformatics Software Professional market agglomerates basically engaged scientific outcomes and noteworthy data taking care of the vital necessities of the report peruses including market members across the worldwide Bioinformatics Software Professional market, financial backers and business visionaries looking for profoundly conclusive examination result…

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Sample OpenAPI – kiota

The following is a minimal OpenAPI description that describes how to call the /me endpoint on Microsoft Graph. This description is used in the following guides. openapi: 3.0.3 info: title: Microsoft Graph get user API version: 1.0.0 servers: – url: graph.microsoft.com/v1.0/ paths: /me: get: responses: 200: description: Success! content: application/json:…

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Uracil-DNA Glycosylase Assay by Matrix-assisted Laser Desorption/Ionization Time-of-flight Mass Spectrometry Analysis

A non-labeled, non-radio-isotopic method to assay uracil-DNA glycosylase activity was developed using MALDI-TOF mass spectrometry for direct apurinic/apyrimidinic site-containing product analysis. The assay proved to be quite simple, specific, rapid, and easy to use for DNA glycosylase measurement. DNA repair mechanisms are very important for maintaining genome integrity in all…

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Batch-effect detection, correction and characterisation in Illumina HumanMethylation450 and MethylationEPIC BeadChip array data | Clinical Epigenetics

Experimental design and processing steps For the EpiSCOPE study [20], DHA supplementation and gender were balanced as much as possible across the 12 450K BeadChips on each glass slide, with these factors also randomly distributed over the 6 rows and 2 columns of 31 slides (Additional file 1: Fig. S1). Blood…

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Mbedtls Application On Tcp

How to use mbedTLS library in Keil or IAR IDEs? I want to do an RSA encryption/decryption using my AT91SAM7S256 ARM MCU.After googling I found mbed TLS library. The IP networking interface includes TLSSockets which behave similarly to normal TCP sockets but automatically use Mbed TLS to set up a…

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Mitogenome-wise codon usage pattern from comparative analysis of the first mitogenome of Blepharipa sp. (Muga uzifly) with other Oestroid flies

Outcome of DNA sequencing, assembly, and validation In this study, initially total DNA was isolated from the finely chopped, full-grown pupa of Blepharipa sp. The NanoDrop spectrophotometer (1294 ng/μl) and the Qubit fluorometer (732.8 ng/μl) both found that the concentration of total DNA in the sample at an optimum level for mitochondrial DNA enrichment. The Tape Station profile showed…

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Restriction Fragment Length Polymorphism – Top Future point

In molecular biology , restriction fragment length polymorphism ( RFLP ) is a technique that exploits variations in homologous DNA sequences, known as polymorphisms , in order to distinguish individuals, populations, or species or to indicate the locations of For in the gene within a sequence. The term may refer…

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Y140591 – YFull YTree Info

R-Y140591 – YFull YTree Info Sample ID Country / Language Info Ref File Testing company Statistics Status YF067865 Germany R-Y140591* —— Hg38 .BAM FTDNA (Y700) 52X, 18.7 Mbp, 151 bp YF076495 Germany R-FT167842 —— Hg38 .BAM FTDNA (Y700) 49X, 18.3 Mbp, 151 bp YF067633 Germany R-FT167842 —— Hg38 .BAM FTDNA…

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biopython – Pipeline for paired end RNA sequence data to proteins

This is a much more complicated question than it might seem. First, you need to understand how RNA-Seq works, and what your data really is. Your “paired-end files” contain reads, which will contain fragments of transcripts. Since those are only fragments of transcripts, you don’t know what frame they are…

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Genome Editing Market Size Value US$ 16.98 billion by 2028; grow at a CAGR of 18.4%

According to our new research study on “Genome Editing Market Forecast to 2028 – COVID-19 Impact and Global Analysis – by Technology, Application, and End User,” the market is expected to grow from US$ 5.19 billion in 2021 to US$ 16.98 billion by 2028; it is estimated to grow at…

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Bernstein wraps postdoc, joins cancer research startup

Tumors are complicated. Scientists can examine individual tumor cells and find varying patterns of gene expression. Sample those same locations again at a later time, and new patterns may emerge. Multiply these experiments by dozens of locations, thousands of genes, and several time points, and now there is a huge…

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Electrocompetent Cells Market to Witness Impressive Growth by 2027

Latest Report Available at Advance Market Analytics, “Electrocompetent Cells Market” provides pin-point analysis for changing competitive dynamics and a forward looking perspective on different factors driving or restraining industry growth. The global Electrocompetent Cells market focuses on encompassing major statistical evidence for the Electrocompetent Cells industry as it offers our…

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Scientific Database Curator at European Molecular Biology Laboratory (EMBL)

About the team/job Metabolomics is the study of small molecule metabolites in the metabolome, which is the complete set of metabolites in a biological sample. By studying these metabolites, we can observe changes in cellular networks and biochemical pathways. This allows us to detect disease states and their progression, monitor…

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Rat Model Market revenue worth USD 2.3 Billion by 2028,

Selbyville, Delaware, April 26, 2022 (GLOBE NEWSWIRE) — The rat model market value is projected to reach USD 2.3 billion by 2028, according to a new research report by Global Market Insights Inc. Growing consumption of personalized medicine and increasing number of clinical trials is expected to augment the market…

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CRISPR Technology Market Size, Share, Demand, Assessment & Opportunity Forecast 2021-2030

Global CRISPR Technology Market Size study, By Product and Service (Hand-Emplaced, Air-Delivered), By Applications (Seismic, Acoustic, Magnetic, Infrared), By End User (Security {Military, Public Security} Critical Infrastructure {Utilities, Industrial & Commercial Facilities}), and Regional Forecasts 2022-2028 Global CRISPR Technology Market is valued approximately USD 1097.66 million in 2021 and is anticipated…

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Electroporation Instruments Market Size 2022 Details such as revenues, market share, strategies, growth rate, product & their pricing by region/country for all major companies Future Growth by 2025

Global Electroporation Instruments Market Report – Production and Consumption Professional Analysis (Impact of COVID-19) This report elaborates the market size, market characteristics, and market growth of the Electroporation Instruments industry, and breaks down according to the type, application, and consumption area of Electroporation Instruments. The report also conducted a PESTEL…

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CRISPR Gene-Editing Market May See a Big Move by 2029

Global Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market Synopsis: The finest Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Gene-Editing Market research report contains the drivers and restraints for the market that are obtained with the help of SWOT analysis, and also shows all the recent developments, product launches,…

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pymc3 TypeError: Unsupported dtype for TensorType: object

return np.log(w * norm.pdf(y, mu1, sigma1) + (1-w) * norm.pdf(y, mu2, sigma2)).sum() because scipy.stats.norm doesn’t know how to deal with pymc/Theano objects. While I’m sure there’s an easy way to get a normal density from pymc3 objects, it’s also straightforward to define a function to calculate it ourselves: def normpdf(y,…

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Semi-Targeted Ultra-High-Performance Chromatography Coupled to Mass Spectrometry Analysis of Phenolic Metabolites in Plasma of Elderly Adults

The goal of this protocol is to detect phenolic metabolites in plasma using a semi-targeted chromatography-mass spectrometry method. The goal of this protocol is to detect phenolic metabolites in plasma by a semi-targeted UPLC-MS/MS method. Plasma proteins were precipitated with ethanol and samples concentrated, and re-suspended in the mobile phase,…

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CTS1346 – YFull YTree Info

Sample ID Country / Language Info Ref File Testing company Statistics Status HGDP01351 China, People’s Republic of O-F3607* —— Hg38 .BAM Scientific 16X, 23.6 Mbp, 151 bp YF079316 —— O-Y224790 —— Hg19 .BAM 23mofang 58X, 21.3 Mbp, 150 bp HG00583 China, People’s Republic of O-Y224790 —— Hg19 .BAM Scientific ——…

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Integrative analysis of eQTL and GWAS summary statistics reveals transcriptomic alteration in Alzheimer brains | BMC Medical Genomics

Significant gene-AD associations With the GWAS summary data from the IGAP and eQTL summary data from BRAINEAC, we performed both SMR and HEIDI tests to estimate the gene-AD associations in three human brain regions: frontal cortex, temporal cortex, and hippocampal regions. For the frontal cortex and hippocampal regions, we obtained…

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Summary of Fusobacterium nucleatum MJR7757B, version 26.0

Summary of Fusobacterium nucleatum, Strain MJR7757B, version 26.0 Tier 3 Uncurated Database Summary: This Pathway/Genome Database (PGDB) was generated on 27-May-2018 from the annotated genome of Fusobacterium nucleatum MJR7757B, as obtained from RefSeq (annotation date: 04-APR-2017). The PGDB was created computationally by the PathoLogic component of the Pathway Tools software…

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Le Roy students studying DNA as part of University at Buffalo project | Lifestyles

BUFFALO — Stephen T. Koury, research associate professor of biotechnical and clinical laboratory sciences, has been awarded a $1.3 million grant that will allow high school students and teachers the opportunity to perform genome sequencing of bacteria. “The Metagenomics Education Partnership: Harnessing the Power of Microbial Genome Sequencing and Big…

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