Tag: SCID

Study finds revolutionary cancer vaccine simultaneously kills and prevents brain cancer

[Dec. 17, 2023: JJ Shavit, The Brighter Side of News] Dual action cell therapy is engineered to eliminate established tumors and train the immune system to eliminate the primary tumor and prevent cancer recurrence. (Credit: Creative Commons) In a bold effort to rewrite the story of cancer treatment, scientists are…

Continue Reading Study finds revolutionary cancer vaccine simultaneously kills and prevents brain cancer

Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition

Patients, tumour Implantation, PDX expansion and characterization The study was conducted according to the Declaration of Helsinki. Tumours were collected from EC patients following Human Research Ethics Committee (HREC) approval (HREC/15/MHS/127) and QUT HREC (#1500000169, 1500000323). All participants provided written informed consent that their excess tissue could be used for…

Continue Reading Endometrial cancer PDX-derived organoids (PDXOs) and PDXs with FGFR2c isoform expression are sensitive to FGFR inhibition

Gene Therapy Market projected to reach USD 38.33 Billion by

Richmond, Dec. 06, 2023 (GLOBE NEWSWIRE) — According to a research report “Gene Therapy Market”, By Technology (Viral Vector-Based Gene Therapy {Adeno-Associated Virus (AAV), Lentivirus, Adenovirus, Retrovirus and Modified Herpes Simplex Virus}, Non-Viral Vectors-Based Gene Therapy {Electroporation, Gene Gun, Liposomes and Naked DNA}, CRISPR/Cas9 Technology), Therapy Type (Acute Lymphoblastic Leukemia…

Continue Reading Gene Therapy Market projected to reach USD 38.33 Billion by

Genetic risk converges on regulatory networks mediating early type 2 diabetes

Kahn, S. E., Hull, R. L. & Utzschneider, K. M. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature 444, 840–846 (2006). Article  ADS  PubMed  Google Scholar  Halban, P. A. et al. β-cell failure in type 2 diabetes: postulated mechanisms and prospects for prevention and treatment. Diabetes Care…

Continue Reading Genetic risk converges on regulatory networks mediating early type 2 diabetes

Mouse Brain Tissue Preparation 8: Normal Nerve Astrocytes | Technique alternative | 01018463296

About Mouse Brain Tissue Preparation Buffer 8: Normal Nerve Astrocytes Check information Catalog number: 9-80131 Full name: Mouse Brain Tissue Preparation Buffer 8: Normal Nerve Astrocytes Size: 1 x 100 ml Supplier:  CHI Scientific Price: 207.00 Shelf life : The shelf life of this product is: 6 mois Storage : The storage of this product is…

Continue Reading Mouse Brain Tissue Preparation 8: Normal Nerve Astrocytes | Technique alternative | 01018463296

Which Is An Application Of DNA Technology In Medicine

Screening for Genetic Disorders Advances in DNA technology have revolutionized the field of medicine, particularly in the area of screening for genetic disorders. Screening plays a crucial role in identifying individuals who may be at risk of inheriting or passing on certain genetic conditions. By analyzing an individual’s DNA, healthcare…

Continue Reading Which Is An Application Of DNA Technology In Medicine

The Power of Diagnosis and Therapy

WASHINGTON DC – In the 2023 Presidential symposium at last week’s American Society of Human Genetics (ASHG) conference, three speakers invited by the Society’s president, Brendan Lee, showcased progress that illustrated, in Lee’s words, “the power of diagnosis and the power of therapy.” “It is not a Sisyphean task,” Lee…

Continue Reading The Power of Diagnosis and Therapy

CRISPR/Cas9-based disease modelling and functional correction of Interleukin 7 Receptor alpha Severe Combined Immunodeficiency in T-lymphocytes and hematopoietic stem cells

Abstract Interleukin 7 Receptor Alpha Severe Combined Immunodeficiency (IL7R-SCID) is a life-threatening disorder caused by homozygous mutations in the IL7RA gene. Defective IL7R expression in humans hampers T cell precursors proliferation and differentiation during lymphopoiesis resulting in absence of T cells in newborns, who succumb to severe infections and death…

Continue Reading CRISPR/Cas9-based disease modelling and functional correction of Interleukin 7 Receptor alpha Severe Combined Immunodeficiency in T-lymphocytes and hematopoietic stem cells

Integrative transcriptome- and DNA methylation analysis of brain tissue from the temporal pole in suicide decedents and their controls

World Health Organization. Facts sheet: suicide. www.who.int/news-room/fact-sheets/detail/suicide. National Institute of Mental Health. Suicide. www.nimh.nih.gov/health/statistics/suicide. Nock MK, Hwang I, Sampson N, Kessler RC, Angermeyer M, Beautrais A, et al. Cross-national analysis of the associations among mental disorders and suicidal behavior: findings from the WHO world mental health surveys. PLoS Med. 2009;6:e1000123….

Continue Reading Integrative transcriptome- and DNA methylation analysis of brain tissue from the temporal pole in suicide decedents and their controls

When Glia Meet Induced Pluripotent Stem Cells (iPSCs)

Abstract The importance of glial cells, mainly astrocytes, oligodendrocytes, and microglia, in the central nervous system (CNS) has been increasingly appreciated. Recent advances have demonstrated the diversity of glial cells and their contribution to human CNS development, normal CNS functions, and disease progression. The uniqueness of human glial cells is…

Continue Reading When Glia Meet Induced Pluripotent Stem Cells (iPSCs)

Novel replacement strategy holds promise for the treatment of various genetic disorders

Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer cell function and counts. Left untreated, SCID proves fatal within the first year of life. The conventional treatment for SCID patients…

Continue Reading Novel replacement strategy holds promise for the treatment of various genetic disorders

Precise gene editing for new therapies

Newswise — Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer cell function and counts. Left untreated, SCID proves fatal within the first year of life. The conventional treatment for…

Continue Reading Precise gene editing for new therapies

CRISPR’s Innovative “Find-and-Replace” Genome Editing

Bar-Ilan University researchers advance gene therapy for genetic disorders like SCIDs using a refined CRISPR-Cas9 technique, named GE x HDR 2.0. Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer…

Continue Reading CRISPR’s Innovative “Find-and-Replace” Genome Editing

Find-and-Replace Genome Editing with CRISPR: A Promising Therapeutic Strategy

Newswise — Severe Combined Immunodeficiencies (SCIDs) are a group of debilitating primary immunodeficiency disorders, primarily caused by genetic mutations that disrupt T-cell development. SCID can also affect B-cell and natural killer cell function and counts. Left untreated, SCID proves fatal within the first year of life. The conventional treatment for…

Continue Reading Find-and-Replace Genome Editing with CRISPR: A Promising Therapeutic Strategy

B4GALT5 shRNA Plasmid Mouse) – Gentaur.com

Catalog number MBS8274761 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…

Continue Reading B4GALT5 shRNA Plasmid Mouse) – Gentaur.com

Macrophage polarization and metabolism in atherosclerosis

Organization WH the top 10 causes of death. www.who.int/news-room/fact-sheets/detail/the-top-10-causes-of-death, 2019. Rana JS, Khan SS, Lloyd-Jones DM, Sidney S. Changes in mortality in Top 10 causes of death from 2011 to 2018. J Gen Intern Med. 2021;36:2517–8. Article  PubMed  Google Scholar  Libby P. The changing landscape of atherosclerosis. Nature. 2021;592:524–33. Article …

Continue Reading Macrophage polarization and metabolism in atherosclerosis

Gene Therapy Market To Soar At 18.5% CAGR, Reaching $10.1 Billion By 2032 Themarketpublicist

(MENAFN– Ameliorate Digital Consultancy) As of 2021, the global gene therapy market was valued at an estimated $1.55 billion USD. This market is poised for significant expansion, with a projected increase from $1.85 billion in 2022 to a remarkable $10.1 billion by 2032, reflecting a robust compound annual growth rate…

Continue Reading Gene Therapy Market To Soar At 18.5% CAGR, Reaching $10.1 Billion By 2032 Themarketpublicist

Genomics England Releases List of over 200 Rare Conditions for Newborn Screening Study

Credit: comzeal/Getty Images Genomics England has published an initial list of over 200 rare conditions that will be screened as a part of its new Generation Study, a program aimed at understanding whether sequencing babies’ genomes through newborn screening can help discover and treat rare genetic conditions earlier. The study will analyze the DNA…

Continue Reading Genomics England Releases List of over 200 Rare Conditions for Newborn Screening Study

Activation of STING by SAMHD1 Deficiency Promotes PANoptosis and Enhances Efficacy of PD-L1 Blockade in Diffuse Large B-cell Lymphoma

Figure 1 SAMHD1 expression is up-regulated in… Figure 1 SAMHD1 expression is up-regulated in DLBCL and related to tumor growth. A, B.… Figure 1 SAMHD1 expression is up-regulated in DLBCL and related to tumor growth. A, B. The mRNA levels of SAMHD1 in DLBCL samples from the Oncomine (A) and…

Continue Reading Activation of STING by SAMHD1 Deficiency Promotes PANoptosis and Enhances Efficacy of PD-L1 Blockade in Diffuse Large B-cell Lymphoma

Alginate Beads as a Tool for Production of Human IPSCs

Introduction There are several promising applications for human-induced pluripotent stem cells (IPSCs), including cell replacement therapies, tissue engineering, and high-throughput toxicological and pharmacological screening.1 The scalable expansion and differentiation of IPSCs are demanding and inefficient; in particular, they commonly require the use of two-dimensional (2D) cultures.2 Data on stem cell…

Continue Reading Alginate Beads as a Tool for Production of Human IPSCs

A biobank of pediatric patient-derived-xenograft models in cancer precision medicine trial MAPPYACTS for relapsed and refractory tumors

Patient characteristics and PDX establishment Between February 2016 and July 2020, 787 pediatric, adolescent and young adult patients with recurrent or refractory malignancies were enrolled in the MAPPYACTS trial;2 756 (96%) patients and their parents consented to the optional ancillary study of preclinical model development (Fig. 1a). 744 patients had a…

Continue Reading A biobank of pediatric patient-derived-xenograft models in cancer precision medicine trial MAPPYACTS for relapsed and refractory tumors

CCDC50, an essential driver involved in tumorigenesis, is a potential severity marker of diffuse large B cell lymphoma

Data collection and bioinformatic analysis GSE10846, GSE19246, GSE32918, GSE50721, GSE64820, and GSE94669, were downloaded from the Gene Expression Omnibus database (www.ncbi.nlm.nih.gov/geo/). DLBCL datasets from the GEPIA (Gene Expression Profiling Interactive Analysis) (gepia.cancer-pku.cn) and the TCGA (The Cancer Genome Atlas) (xenabrowser.net/datapages/) were also used in this study. A total of 284…

Continue Reading CCDC50, an essential driver involved in tumorigenesis, is a potential severity marker of diffuse large B cell lymphoma

MCMBP shRNA Plasmid Mouse) – Gentaur.com

Catalog number MBS8280724 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…

Continue Reading MCMBP shRNA Plasmid Mouse) – Gentaur.com

Gut Microbiota Metabolites Mediate Bax to Reduce Neuronal Apoptosis via cGAS/STING Axis in Epilepsy

Zack MM, Kobau R (2017) National and state estimates of the numbers of adults and children with active epilepsy – United States, 2015. MMWR Morb Mortal Wkly Rep 66:821–825. doi.org/10.15585/mmwr.mm6631a1 Article  PubMed  PubMed Central  Google Scholar  Pottoo F et al (2020) Impact of adherence to antiepileptic medications on quality of…

Continue Reading Gut Microbiota Metabolites Mediate Bax to Reduce Neuronal Apoptosis via cGAS/STING Axis in Epilepsy

Solved (2 pts) You have made iPSCs from a mouse of the

Transcribed image text: (2 pts) You have made iPSCs from a mouse of the C57BL6 strain and are now injecting some of them into a skin fold on the back of a C57BL6 mouse. A teratoma forms that is about \\( 20 \\mathrm{~mm} 3 \\) in volume. You are also…

Continue Reading Solved (2 pts) You have made iPSCs from a mouse of the

Potential immunosuppressive clonal hematopoietic mutations in tumor infiltrating immune cells in breast invasive carcinoma

Our approach for identifying potential immunosuppressive CH mutations in TII consisted of four stages as shown in Fig. 2. (1) We selected protein altering mutations, which are more likely to be pathogenic than non-coding and synonymous mutations. (2) Clonally expanded somatic mutations in TII were identified based on variant allele fraction…

Continue Reading Potential immunosuppressive clonal hematopoietic mutations in tumor infiltrating immune cells in breast invasive carcinoma

Strategies to therapeutically modulate cytokine action

Cohen, S., Bigazzi, P. E. & Yoshida, T. Commentary. Similarities of T cell function in cell-mediated immunity and antibody production. Cell Immunol. 12, 150–159 (1974). Article  CAS  PubMed  Google Scholar  Keegan, A. D. & Leonard, W. J. in Paul’s Fundamental Immunology 8th edn, ch. 9 (eds Flajnik, M. F., Singh,…

Continue Reading Strategies to therapeutically modulate cytokine action

In Vivo Genome Editing of Stem Cells Induced by LNP-based Delivery of mRNA

Part of the job as a pediatric hematologist for Michael P. Triebwasser, MD, PhD, is to take care of patients during their bone marrow transplantation. Just last week, he took care of a patient with a severe combined immunodeficiency (SCID) disorder and another with a bone marrow disorder. Even though…

Continue Reading In Vivo Genome Editing of Stem Cells Induced by LNP-based Delivery of mRNA

Synthetic Biology Market to Surge USD 48.81 Billion, with Excellent CAGR of 22.70% by 2029

Global Synthetic Biology Market research report is a comprehensive synopsis on the study of Healthcare industry and its influence on the market environment. Some of the competitor strategies can be mentioned here as new product launches, expansions, agreements, partnerships, joint ventures, and acquisitions. This market report is a clear-cut solution…

Continue Reading Synthetic Biology Market to Surge USD 48.81 Billion, with Excellent CAGR of 22.70% by 2029

Successful treatment with doxycycline monotherapy for human infection with Babesia venatorum (Babesiidae, Sporozoa) in China: a case report and proposal for a clinical regimen | Infectious Diseases of Poverty

Clinical course of the case A 73-year-old male underwent splenectomy after a traffic accident in 2014, and blood transfusion was performed during surgery. He had no history suggestive of any chronic diseases, or drug or alcohol abuse. The patient, with no recorded family history, had quit smoking 10 years ago…

Continue Reading Successful treatment with doxycycline monotherapy for human infection with Babesia venatorum (Babesiidae, Sporozoa) in China: a case report and proposal for a clinical regimen | Infectious Diseases of Poverty

AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells

Hayes, C. Cellular immunotherapies for cancer. Ir. J. Med. Sci. 190, 41–57 (2021). Article  PubMed  Google Scholar  Laskowski, T. & Rezvani, K. Adoptive cell therapy: living drugs against cancer. J. Exp. Med. 217, e20200377 (2020). Article  CAS  PubMed  PubMed Central  Google Scholar  June, C. H., O’Connor, R. S., Kawalekar, O….

Continue Reading AAV-mediated delivery of a Sleeping Beauty transposon and an mRNA-encoded transposase for the engineering of therapeutic immune cells

Gene Therapy Market is booming With CAGR of 18.5% To Reach

Rockville , July 10, 2023 (GLOBE NEWSWIRE) — As per Fact.MR, a provider of market research and competitive intelligence, the global gene therapy market is expected to witness a CAGR of 18.5% during the forecast period due to a significant increase in the number of cases of rare diseases. The…

Continue Reading Gene Therapy Market is booming With CAGR of 18.5% To Reach

Different US/UK Initiatives Each Plan to Sequence the Genomes of 100,000 Newborns to Identify Treatable Rare Diseases Undetectable to Standard Screening Tests

Both programs seek to achieve early diagnosis by detecting a range of disorders where an existing treatment can be given as early as possible Two separate genetic sequencing projects—one in the United Kingdom and one in New York City—aim to perform whole-genome sequencing for clinical laboratory diagnostic purposes on 100,000…

Continue Reading Different US/UK Initiatives Each Plan to Sequence the Genomes of 100,000 Newborns to Identify Treatable Rare Diseases Undetectable to Standard Screening Tests

Gene Therapy Breakthrough: Promising Treatment for Sickle Cell Disease and Beta Thalassemia

Sickle Cell Disease (SCD) and Beta Thalassemia are widespread blood disorders that pose significant health risks to millions of individuals worldwide. In recent research conducted by scientists at St. Jude Children’s Research Hospital and the Broad Institute of MIT and Harvard, a revolutionary gene editing technology called adenosine base editing…

Continue Reading Gene Therapy Breakthrough: Promising Treatment for Sickle Cell Disease and Beta Thalassemia

SARDH shRNA Plasmid Mouse) – Gentaur.com

Catalog number MBS8280461 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…

Continue Reading SARDH shRNA Plasmid Mouse) – Gentaur.com

How long until effective Helicobacter pylori vaccine – human

Introduction Helicobacter pylori became a concern in 1979 when John Warren, a pathologist in Perth, Western Australia, discovered that the inflamed gastric mucosa was covered with bacteria that looked like Campylobacter.1 Warren and his colleague Barry Marshall cultured the first batch of these bacteria, newly named Helicobacter pylori, subsequently from…

Continue Reading How long until effective Helicobacter pylori vaccine – human

A New Frontier in Treating Inherited Diseases

Gene Therapy: A New Frontier in Treating Inherited Diseases Gene therapy, a groundbreaking medical approach that has been gaining momentum in recent years, offers the potential to revolutionize the treatment of inherited diseases. By directly targeting the root cause of these conditions – the faulty genes themselves – gene…

Continue Reading A New Frontier in Treating Inherited Diseases

Exogenously delivered iPSCs disrupt the natural repair response of endogenous MPCs after bone injury

Ethics statement All animal studies were performed in accordance with the recommendations in the Canadian Council on Animal Care Guidelines. The reporting of this data in the manuscript follows the recommendations in the ARRIVE guidelines. The University of Calgary Health Sciences Animal Care Committee approved all animal protocols and surgical…

Continue Reading Exogenously delivered iPSCs disrupt the natural repair response of endogenous MPCs after bone injury

Viral Vectors and Plasmid DNA Manufacturing Market

The viral vectors and plasmid DNA manufacturing market is projected to expand at a CAGR of 22.5%, during the period from 2023 to 2031, propelled by the rising demand for advanced gene therapies, vaccines, and cell therapies. With a potential compound annual growth rate (CAGR), the market’s revenue is expected…

Continue Reading Viral Vectors and Plasmid DNA Manufacturing Market

Human bone marrow-derived mesenchymal stem overexpressing microRNA-124-3p inhibit DLBCL progression by downregulating the NFATc1/cMYC pathway | Stem Cell Research & Therapy

Microarray-based expression analysis and GEO database analysis The DLBCL-related miRNA expression profiles of GSE29493 and GSE40239 were retrieved from the Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo/). MiR-124’s pan cancer analysis is conducted through online websites (www.picb.ac.cn/dbDEMC/). Bioinformatics predicted NFATc1 as the target of miR-124-3p The miRbase (www.mirbase.org/), miRDB (mirdb.org), and…

Continue Reading Human bone marrow-derived mesenchymal stem overexpressing microRNA-124-3p inhibit DLBCL progression by downregulating the NFATc1/cMYC pathway | Stem Cell Research & Therapy

IL5RA as an immunogenic cell death-related predictor in progression and therapeutic response of multiple myeloma

Differential expression analysis We downloaded GSE125361 (n = 48) microarray data from the Gene Expression Omnibus (GEO) database, which included 45 myeloma samples and 3 controls, for expression analysis of IL5RA in cancer16. Additionally, we analyzed the expression of IL5RA in smoldering myeloma (SMM) patients who progressed to active MM (n = 10) and…

Continue Reading IL5RA as an immunogenic cell death-related predictor in progression and therapeutic response of multiple myeloma

VCL shRNA Plasmid Mouse) – Gentaur.com

Catalog number MBS8273409 Price Please ask Size 0,15 mg Other size please contact us to order other different size Test Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40…

Continue Reading VCL shRNA Plasmid Mouse) – Gentaur.com

Identification of acquired Notch3 dependency in metastatic Head and Neck Cancer

Molecular profiling reveals common changes in matched pairs of HNSCC primary tumor and metastasis-derived cell lines Identification of both genetic and functional differences between primary and metastatic variants of HNSCC would be enabled by in vitro models derived independently from these sites from the same patient. Unfortunately, HNSCC tumor cells…

Continue Reading Identification of acquired Notch3 dependency in metastatic Head and Neck Cancer

Mice Model Technologies Market Poised for 7.4% CAGR Growth, Reaching USD 3.2 Bn by 2031

Transparency Market Research North America to provide significant mice model technologies market opportunities to regional and global manufacturers. High incidence of genetic disorders worldwide has led the significant Research and Development investment in genetic testing and a rise in the adoption of CRISPR technology in the healthcare industry. Wilmington, Delaware,…

Continue Reading Mice Model Technologies Market Poised for 7.4% CAGR Growth, Reaching USD 3.2 Bn by 2031

Synthetic Biology Market is Poised to Thrive at CAGR of

Wilmington, Delaware, United States, April 20, 2023 (GLOBE NEWSWIRE) — The global synthetic biology market is anticipated to grow at a 21.4% CAGR from 2023 to 2031. A market valuation of US$ 74.7 billion is expected by end of the forecast period. The mounting scope of synthetic biology for multiplexed…

Continue Reading Synthetic Biology Market is Poised to Thrive at CAGR of

Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice

Optimizing prime editing systems for HSPCs We previously reported the use of prime editing to correct HBBS by plasmid transfection in HEK293T cells containing the SCD mutation, reaching up to 58% efficiency (Fig. 1a)24. In contrast to HEK293T cells, HSPCs are difficult to transfect with plasmid DNA but are amenable…

Continue Reading Ex vivo prime editing of patient haematopoietic stem cells rescues sickle-cell disease phenotypes after engraftment in mice

Direct reprogramming of human fibroblasts into insulin-producing cells using transcription factors

Exogenous expression of the transcription factors Pdx1, Neurog3, and MafA in human fibroblasts We first sought to examine whether the transcription factors Neurog3, Pdx1, and MafA could induce expression of the INSULIN (INS) gene in human fibroblasts as readout of the capacity of these cells to be transformed toward a…

Continue Reading Direct reprogramming of human fibroblasts into insulin-producing cells using transcription factors

Study uses base editing to correct mutation that causes rare immune deficiency — ScienceDaily

A new UCLA-led study suggests that advanced genome editing technology could be used as a one-time treatment for the rare and deadly genetic disease CD3 delta severe combined immunodeficiency. The condition, also known as CD3 delta SCID, is caused by a mutation in the CD3D gene, which prevents the production…

Continue Reading Study uses base editing to correct mutation that causes rare immune deficiency — ScienceDaily

Base Editing Shows Promise for Treating Rare Immune Disorder

Credit: CIPhotos/Getty Images Research led by the University of California, Los Angeles, shows base editing could be used to treat a rare immune disorder known as CD3 delta severe combined immunodeficiency (SCID). Writing in the journal Cell, the authors describe using base editing, a new and more precise version of…

Continue Reading Base Editing Shows Promise for Treating Rare Immune Disorder

Isoform-specific knockdown of long and intermediate prolactin receptors interferes with evolution of B-cell neoplasms

Knockdown of LFPRLR reduces splenic B-cell subsets in SLE-prone mice To investigate whether the PRL-LFPRLR axis raises the risk of initiation of B-cell malignancies, we compared SLE-prone MRL-lpr mice treated with either control SMO or LFPRLR SMO. Among SLE-prone models, we chose MRL-lpr mice because they accumulate genetic lesions indicative…

Continue Reading Isoform-specific knockdown of long and intermediate prolactin receptors interferes with evolution of B-cell neoplasms

Inactivation of interleukin-30 in colon cancer stem cells via CRISPR/Cas9 genome editing inhibits their oncogenicity and improves host survival

Introduction Colorectal cancer (CRC) is a leading cause of cancer-related death1 and its mortality rate is expected to rise worldwide, due to population growth and aging, thus entailing a global public health challenge. CRC mortality is mainly due to therapy resistance and metastasis, which are driven by a small population…

Continue Reading Inactivation of interleukin-30 in colon cancer stem cells via CRISPR/Cas9 genome editing inhibits their oncogenicity and improves host survival

Induction of iPSC-derived Prg4-positive cells with characteristics of superficial zone chondrocytes and fibroblast-like synovial cells | BMC Molecular and Cell Biology

Lubricin is believed to be an effective therapeutic agent for osteoarthritis and synovitis. However, clinical application of recombinant or synthesised lubricin has not been attempted yet. In this study, we successfully developed a protocol to induce lubricin-expressing cells from pluripotent stem cells and confirmed their differentiation into Prg4-expressing cells that…

Continue Reading Induction of iPSC-derived Prg4-positive cells with characteristics of superficial zone chondrocytes and fibroblast-like synovial cells | BMC Molecular and Cell Biology

biotechnology jobs in italy

Biotechnology Jobs: Here’re the available jobs in the biotechnology field. 96 jobs to view and apply for now with New Scientist Jobs NextPharmaJob.com is dedicated to putting job seekers looking for pharmaceutical industry jobs in touch with recruiters and employers. Find job openings from companies that are hiring now (and…

Continue Reading biotechnology jobs in italy

The Clinical Importance of Immunogenetics

Immunogenetics describes the investigation of the genetics underpinning the immune system and its responses. Its overall goal is to identify the specific genes responsible for encoding immunological cells and molecules. DNA. Image Credit: vitstudio/Shutterstock.com Linking genetics with immune dysregulation Immunogenetics is highly pertinent to a plethora of…

Continue Reading The Clinical Importance of Immunogenetics

MYO10 drives genomic instability and inflammation in cancer

INTRODUCTION Genomic instability often refers to the existence of a variety of DNA alterations, ranging from single nucleotide changes (such as base substitution, deletion, and insertion) to chromosomal rearrangements (e.g., gain or loss of a segment or the whole chromosome) (1). Loss of genome stability can lead to early onset…

Continue Reading MYO10 drives genomic instability and inflammation in cancer

CRISPR Applications in Medicine Depend on Minimizing Off-Target Editing

By Rolf Turk, PhD Genome editing technologies, such as CRISPR, are being applied to better understand basic biological systems as well as to research new kinds of gene and cell therapies. The CRISPR-Cas9 system comprises a Cas9 endonuclease protein that forms a complex with a guide RNA (gRNA) molecule, which…

Continue Reading CRISPR Applications in Medicine Depend on Minimizing Off-Target Editing

Humanized Mice: Generation, Advantages and Applications

Mice are commonly used as models of human disease, helping scientists to understand the workings of complex pathologies and develop safe and efficacious drugs. It is now possible to create a range of genetically engineered mouse models, including humanized, knock-in and knock-out models, that can be used to further advance…

Continue Reading Humanized Mice: Generation, Advantages and Applications