Tag: sEVs

Rapid purification and characterization of circulating small extracellular vesicles on a label-free lab-on-a-chip

Device layout and graphical outline of experimental flow. Small extracellular vesicles (sEVs) were simultaneously extracted from human biofluids using the insulator-based dielectrophoretic (iDEP) device, wherein a trapping zone was created at the tip region of micropipettes by balancing the dielectrophoretic (DEP) force with two electrokinetic forces, including electroosmosis (EOF) and…

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Ambra1+ Myo-sEVsI/R enriched with mitochondri

image: Ambra1+-MYO-SEVS WERE INTERNALIZED BY FIBROBLASTS AND THE DELIVERED mtDNA COMPONENTS TO ACTIVATE THE FIBROBLAST cGAS-STING PATHWAY TO PROMOTE FIBROBLAST ACTIVATION AND PROLIFERATION. view more  Credit: ©Science China Press This study is led by Prof. Heng Ma (School of Basic Medicine, Fourth Military Medical University), Prof. Alex Chia Yu Chang (Ninth…

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A new communication medium between cardiomyocytes and fibroblasts

Ambra1+-Myo-sEVs were internalized by fibroblasts and the delivered mtDNA components to activate the fibroblast cgas-sting pathway to promote fibroblast activation and proliferation. Credit: Science China Press Myocardial fibrosis is major pathological outcome after myocardial ischemia/reperfusion (MI/R) injury and a major risk factor for heart failure. MI/R injury induces cardiomyocyte damage…

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Plant-derived nanovesicles facilitate tumor therapy

Introduction Tumors are the second leading cause of death worldwide, with approximately 1670 deaths per day in the United States and 7700 deaths per day in China.1,2 The fundamental reason why cancers are still considered “frightening” is the difficulty of early tumor detection and the variety of issues present in…

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A simple and available measurement of onco-sEV dsDNA to protein ratio as a potential tumor marker | BMC Cancer

Research object In this study, 34 tumor patients treated in Nanjing Drum Tower Hospital from May 2021 to September 2021 were selected. The average age of tumor group was 59 years old, with 25 male and 9 female. There were 13 cancer types in cancer group, including gastric cancer, lung…

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Exosomal circRNA: emerging insights into cancer progression and clinical application potential | Journal of Hematology & Oncology

He G, Peng X, Wei S, Yang S, Li X, Huang M, et al. Exosomes in the hypoxic TME: from release, uptake and biofunctions to clinical applications. Mol Cancer. 2022;21:19. Article  PubMed  PubMed Central  Google Scholar  Downs-Canner SM, Meier J, Vincent BG, Serody JS. B cell function in the tumor…

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IJMS | Free Full-Text | Impact of Circulating Cell-Free DNA (cfDNA) as a Biomarker of the Development and Evolution of Periodontitis

1. Introduction Periodontitis is a chronic disease that affects 10% of the world’s population [1]. It is characterized by the interaction among bacterial, inflammation and genetic factors. Specific virulent oral microbials cause the host immune response in patients with a genetic predisposition. The inflammation of the periodontal tissues could result,…

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Optimization of Flow Cytometric Sorting Parameters for High-Throughput Isolation and Purification of Small Extracellular Vesicles

This protocol provides a rapid and size-specific isolation method for small extracellular vesicles by optimizing the size of the air spray nozzle, sheath fluid pressure, sample flow pressure, voltage, gain, and triggering threshold parameters. This protocol provides a rapid and size-specific solution method for small extracellular vesicles by optimizing flow…

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A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs

Mechanistic studies of the EXTRA-CRISPR assay The assay is designed to be a tri-enzymatic cascade that exploits the unique nucleolytic cleavage activities of the CRISPR–Cas12a system to create a new exponential isothermal amplification mechanism based on the robust linear RCA assay. The assay starts with the hybridization of a padlock…

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Small Extracellular Vesicles Derived from Induced Pluripotent Stem Cells in the Treatment of Myocardial Injury

Induced pluripotent stem cell (iPSC) therapy brings great hope to the treatment of myocardial injuries, while extracellular vesicles may be one of the main mechanisms of its action. iPSC-derived small extracellular vesicles (iPSCs-sEVs) can carry genetic and proteinaceous substances and mediate the interaction between iPSCs and target cells. In recent…

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