Tag: SmartSeq

eli5 RNA Spike-ins : explainlikeimfive

I’m a data scientist trying to branch out into more bioinformatics/genetics. My tutorial is counting spike-ins, but his explanation was over my head: 1 – AnnData and Preprocessing spike-ins | Kaggle ” Because this is smartseq2 data, we may have spike-ins. An RNA spike-in is an RNA transcript of known…

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Re-analysis of multiple scRNAseq data as a combined new one

Re-analysis of multiple scRNAseq data as a combined new one 0 Hi all, There are so many regions in brain.The cortex itself is of many types.I found many single cell RNA seq studies which focus on different regions eg- gse … somatosensory cortex dropseq year 2015 gse….. primary visual cortex…

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Differences between SMART-seq2, SMART-seq3, and 10x

Forum:Differences between SMART-seq2, SMART-seq3, and 10x 0 Hi everyone, I’ve recently started analyzing single-cell RNA-seq data (with FASTQ files as a starting point) and so far I have used 10x genomics data from their website. Now, I’m interested in using data generated by other protocols, specifically SMART, because it is…

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SmartSeq vs SmartSeq2

SmartSeq vs SmartSeq2 0 My data is SmartSeq data, and I would like to run the velocyto command for SmartSeq2 to carry out the initial conversion step for RNA velocity analysis. My question is quite simple: what is the difference between smartseq and smartseq2? Is this difference large enough to…

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