Tag: ssDNA
Building Modified DNA topology – User discussions
GROMACS version: 2021.4GROMACS modification: Yes/NoHere post your question I am having trouble generating topology for a type of modified DNA, but I’m not sure how to do it.In fact, there is a 35-nucleotide sequence of ssDNA. Using the Discovery Studio program, I converted the Thymidine Oxygen 4 atom to Sulfur…
Efficient target cleavage by Type V Cas12a effectors programmed with split CRISPR RNA
doi: 10.1093/nar/gkab1227. Online ahead of print. Regina Shebanova 1 , Natalia Nikitchina 1 2 , Nikita Shebanov 1 , Vladimir Mekler 3 , Konstantin Kuznedelov 3 , Egor Ulashchik 4 , Ruslan Vasilev 5 6 , Olga Sharko 4 , Vadim Shmanai 4 , Ivan Tarassov 2 , Konstantin Severinov 1 3 7 , Nina Entelis 2…
Selection of DNA Aptamers Recognizing EpCAM-Positive Prostate Cancer b
Introduction Prostate cancer (PCa) is one of the most common genitourinary system malignant tumor in men worldwide. In Asian countries, many patients with PCa are often diagnosed at an advanced stage maybe mostly because of a large population base with relatively backward economic development and imperfect cancer screening system of…
Unveiling the Microscopic Mechanism of Current Variation in the Sensing Region of the MspA Nanopore for DNA Sequencing
Different nucleotides generate specific ionic currents that discriminate between the nucleotides while they are passing through the nanopore constriction. MspA is a commonly used nanopore for DNA sequencing. However, the reasons of the current variation remain ambiguous. Our work unveils the microscopic mechanism of current variation for an ssDNA passing…
UChicago Spin-Out AccuraDX Launches KAS-seq Research Kits: ‘A New Way to Study Important Biological Processes and Regulatory Pathways’
Published on Tuesday, September 14, 2021 The KAS-Direct™ ssDNA Labeling and Enrichment Kit, Version 1. (Image credit: AccuraDX) A University of Chicago spin-out company AccuraDX this week has launched its KAS-Direct™ single-stranded DNA (ssDNA) Labeling and Enrichment Kit. Founded in 2020, the company, which is headquartered in Natick, MA with an…
Short Read-Length Next Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs
doi: 10.1007/978-1-0716-1450-1_6. Affiliations Expand Affiliations 1 Department of Pathology and Lab Medicine, University of Saskatchewan, Saskatoon, SK, Canada. 2 Department of Pathology and Lab Medicine, University of Saskatchewan, Saskatoon, SK, Canada. ron.geyer@usask.ca. Item in Clipboard Landon Pastushok et al. Methods Mol Biol. 2022. Show details Display options Display options Format AbstractPubMedPMID…
CRISPR: Guide to gRNA design
Introduction to CRISPR in SnapGene Genome editing technology has been evolving for many years. The Holy Grail of genome engineering has always been to introduce a specific genetic change that affects only the genomic target and leaves no undesired changes in the DNA. The discovery and application of the bacterial…
