Tag: ssDNA

NP1 mutants that do not interact with Ku70 and RPA70 in vitro lose the function in facilitating HBoV1 DNA replication in HEK293 cells.

(A) Schematic digram of the NP1 protein. NP1 protein is schematically diagramed with predicated helical strutrues. The amino acid sequences of aa90-219 are shown with indication of each helix. The potential functional motifs (underlined) within each helical struture were predicted with Eukaryotic Linear Motif resource. NP1 mutants that bear mutaions…

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When the strands of dsdna are separated this is called?

You are learning about: “When the strands of dsdna are separated this is called?”. This is a “hot” question with 2,820,000 searches/month. Let’s fleetserviceshocrv.com learn more about When the strands of dsdna are separated this is called? in this article. How do scientists name the ends of the DNA strands?…

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CRISPR/Cas12a-Enhanced Loop-Mediated Isothermal Amplification for the Visual Detection of Shigella flexneri

doi: 10.3389/fbioe.2022.845688. eCollection 2022. Affiliations Expand Affiliations 1 Provincial Key Laboratory for Transfusion-Transmitted Infectious Diseases, Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College, Chengdu, China. 2 Clinical Laboratory Department, Yan’an Hospital Affiliated to Kunming Medical University, Kunming, China. 3 Department of Emergency, The Traditional…

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Structure of the human RAD17-RFC clamp loader and 9-1-1 checkpoint clamp bound to a dsDNA-ssDNA junction

Abstract The RAD9-RAD1-HUS1 (9-1-1) clamp forms one half of the DNA damage checkpoint system that signals the presence of substantial regions of single-stranded DNA arising from replication fork collapse or resection of DNA double strand breaks. Loaded at the 5′-recessed end of a dsDNA-ssDNA junction by the RAD17-RFC clamp loader…

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Development of Cas12a-Based Cell-Free Small-Molecule Biosensors via Allosteric Regulation of CRISPR Array Expression

In nature, microbes have evolved different systems to sense external stimuli. Synthetic biology approaches (1) repurpose these systems as biosensors to specifically and sensitively detect various targets of interest. Although various highly sensitive and specific laboratory-based analytical methods (including high-performance liquid chromatography and mass spectrometry) can detect small-molecule targets, they…

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Virology: Module 2 Flashcards | Quizlet

Virology: Module 2 Flashcards | Quizlet   attachment/entry, translation of viral mRNA into viral proteins, replication, assembly, release of viruses from cell steps of infection for viruses? a cell possesses the functional receptor for a virus – virus can bind, possibly enter, but replication isn’t guaranteed a cell does not…

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Building Modified DNA topology – User discussions

GROMACS version: 2021.4GROMACS modification: Yes/NoHere post your question I am having trouble generating topology for a type of modified DNA, but I’m not sure how to do it.In fact, there is a 35-nucleotide sequence of ssDNA. Using the Discovery Studio program, I converted the Thymidine Oxygen 4 atom to Sulfur…

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Efficient target cleavage by Type V Cas12a effectors programmed with split CRISPR RNA

doi: 10.1093/nar/gkab1227. Online ahead of print. Regina Shebanova  1 , Natalia Nikitchina  1   2 , Nikita Shebanov  1 , Vladimir Mekler  3 , Konstantin Kuznedelov  3 , Egor Ulashchik  4 , Ruslan Vasilev  5   6 , Olga Sharko  4 , Vadim Shmanai  4 , Ivan Tarassov  2 , Konstantin Severinov  1   3   7 , Nina Entelis  2…

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Selection of DNA Aptamers Recognizing EpCAM-Positive Prostate Cancer b

Introduction Prostate cancer (PCa) is one of the most common genitourinary system malignant tumor in men worldwide. In Asian countries, many patients with PCa are often diagnosed at an advanced stage maybe mostly because of a large population base with relatively backward economic development and imperfect cancer screening system of…

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Unveiling the Microscopic Mechanism of Current Variation in the Sensing Region of the MspA Nanopore for DNA Sequencing

Different nucleotides generate specific ionic currents that discriminate between the nucleotides while they are passing through the nanopore constriction. MspA is a commonly used nanopore for DNA sequencing. However, the reasons of the current variation remain ambiguous. Our work unveils the microscopic mechanism of current variation for an ssDNA passing…

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UChicago Spin-Out AccuraDX Launches KAS-seq Research Kits: ‘A New Way to Study Important Biological Processes and Regulatory Pathways’

Published on Tuesday, September 14, 2021 The KAS-Direct™ ssDNA Labeling and Enrichment Kit, Version 1. (Image credit: AccuraDX) A University of Chicago spin-out company AccuraDX this week has launched its KAS-Direct™ single-stranded DNA (ssDNA) Labeling and Enrichment Kit. Founded in 2020, the company, which is headquartered in Natick, MA with an…

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Short Read-Length Next Generation DNA Sequencing of Antibody CDR Combinations from Phage Selection Outputs

doi: 10.1007/978-1-0716-1450-1_6. Affiliations Expand Affiliations 1 Department of Pathology and Lab Medicine, University of Saskatchewan, Saskatoon, SK, Canada. 2 Department of Pathology and Lab Medicine, University of Saskatchewan, Saskatoon, SK, Canada. ron.geyer@usask.ca. Item in Clipboard Landon Pastushok et al. Methods Mol Biol. 2022. Show details Display options Display options Format AbstractPubMedPMID…

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CRISPR: Guide to gRNA design

Introduction to CRISPR in SnapGene Genome editing technology has been evolving for many years. The Holy Grail of genome engineering has always been to introduce a specific genetic change that affects only the genomic target and leaves no undesired changes in the DNA. The discovery and application of the bacterial…

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