Tag: ssDNA

Enhancement and inactivation effect of CRISPR/Cas12a via extending hairpin activators for detection of transcription factors

Li Q, Song ZL, Zhang YX, Zhu LN, Yang Q, Liu XF, Sun XF, Chen XX, Kong RM, Fan GC, Luo XL (2023) Synergistic Incorporation of two ssDNA activators enhances the trans-cleavage of CRISPR/Cas12a. Anal Chem 95:8879–8888 Article  CAS  PubMed  Google Scholar  Ma JY, Wang SY, Du YC, Wang DX,…

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Molecular characterization of a tetra segmented ssDNA virus infecting Botrytis cinerea worldwide | Virology Journal

Analysis of the multisegmented nature of BcssDV1 genome B. cinerea field isolates were previously obtained from infected grapes of vineyards of Italy and Spain and their mycovirome was determined [8]. A new ssDNA virus (BcssDV1, Genbank accession no. MN625247) was discovered and characterized. The sequence previously characterized of BcssDV1 (from…

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Biosensors | Free Full-Text | DNA Probes for Cas12a-Based Assay with Fluorescence Anisotropy Enhanced Due to Anchors and Salts

3.1. Design of Experiments To search optimal structures of ssDNA probes with anchors, we operated with the following assumptions: (1) the anchor should be an easily accessible compound, (2) it should be readily incorporated into the ssDNA probe, (3) it should provide a significant difference in FA before and after…

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Nanoprobe with a barcode | EurekAlert!

Protein-splitting enzymes play an important role in many physiological processes. Such proteases are generally present in an inactive state, only becoming activated under certain conditions. Some are linked to diseases like infections or cancer, making it important to have methods that can selectively detect active proteases. In the journal Angewandte…

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Viruses under the Antarctic Ice Shelf are active and potentially involved in global nutrient cycles

Rignot, E., Jacobs, S., Mouginot, J. & Scheuchl, B. Ice-shelf melting around antarctica. Science (80-) 341, 266–270 (2013). Article  ADS  CAS  Google Scholar  Wadham, J. L. et al. Ice sheets matter for the global carbon cycle. Nat. Commun. 10, 1–17 (2019). Google Scholar  Adusumilli, S., Fricker, H. A., Medley, B.,…

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Light-Triggered Signal Enhancement Strategy Integrated with a CRISPR/Cas13a-Based Assay for Ultrasensitive and Specific miRNA Detection

The development of facile, accurate, and affordable assays for microRNAs (miRNAs) in early cancer is greatly desirable but encounters an obstacle due to low cellular abundance in biofuids. In this study, we present a novel approach called a light-triggered exponential amplification strategy coupled with a CRISPR/Cas13a-based diagnostic system (LEXPA-CRISPR), which…

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Human RAD52 stimulates the RAD51-mediated homology search

Introduction Homologous recombination (HR) is an evolutionarily conserved process that plays a pivotal role in genome stability, diversity, and plasticity. HR is indeed a key repair pathway able to faithfully repair DNA damages including double-strand breaks (DSBs) and DNA gaps by copying the error-free information from the template DNA normally…

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Foods | Free Full-Text | Isothermal Amplification and CRISPR/Cas12a-System-Based Assay for Rapid, Sensitive and Visual Detection of Staphylococcus aureus

1. Introduction Staphylococcus aureus [1], one of the top five foodborne pathogens, has a common and strong aggressiveness in humans and can secrete multiple toxic proteins (Pathogenic enterotoxins, Hemolysin, PVL) [1,2] which can cause bacteraemia, endocarditis, meningitis, toxic shock syndrome, pneumonia and other dangerous infectious diseases [3]. Moreover, the worldwide…

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From Metagenomics to Ecogenomics: NGS-Based Approaches for Discovery of New Circular DNA Single-Stranded Viral Species

doi: 10.1007/978-1-0716-3515-5_7. Affiliations Expand Affiliations 1 Departamento de Biotecnología Agrícola, Instituto Politécnico Nacional, CIIDIR Unidad Sinaloa, Guasave, Sinaloa, Mexico. 2 Department of Plant Biochemistry, Center for Plant Molecular Biology (ZMBP), Eberhard Karls University, Tübingen, Germany. 3 Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora”, Universidad de Málaga-Consejo Superior de Investigaciones…

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Can DNA and 3D classification protocols be used to solve cases of deceased and unidentified women?

How to identifie ddDNA and sdDNA?5 answersDouble-stranded DNA (dsDNA) and single-stranded DNA (ssDNA) can be identified using different methods. One approach is the use of peptide nucleic acid (PNA) for the direct recognition of dsDNA. Another method involves quantitating dsDNA in an aqueous sample solution by measuring its fluorescence intensity….

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Choose the correct option below that most accurately

Choose the correct option below that most accurately labels the boxes in the following figure describing Sanger Sequencing (in alphabetica dATP ddCTP dGTP ddGTP dDTTP DTTP DdATP ACTP TGCAAGTCA Longer fragments C T G A A C G T Original sequence must be: ssDNA template / DNA Polymerase / Synthesis…

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CRISPR enhanced nanotweezers for ultra-precise DNA manipulation and detection

Nov 27, 2023 (Nanowerk News) Since the concept of optical trapping was first proposed in the 1960s and 1970s by Arthur Ashkin, researchers have pursued innovative techniques to precisely manipulate and analyze individual biomolecules like DNA. Such capabilities would revolutionize genetics, molecular biology, and medicine by enhancing our understanding of…

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Empowering optical tweezers with ‘biometric eyes’

a, The diagrammatic sketch of the three components in the solution: DNA@AuNS conjugate, CRISPR/Cas12a complex, and target ssDNA. b, Optical setup, the BS, SPF, and TL are beam splitter, short pass filter, and tube lens (f=200 mm), respectively. Additional details of the setup are provided in the Materials and Methods…

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CRISPR-powered optothermal nanotweezers allow targeted manipulation of single DNA molecules

Nov 22, 2023 (Nanowerk News) For decades, researchers have sought ways to precisely manipulate and identify individual molecules like DNA in liquid environments. Such capabilities could revolutionize areas ranging from disease diagnosis to drug development. However, the randomness of molecular movements in fluids has hindered progress. Now, scientists from Shenzhen…

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Lung and liver editing by lipid nanoparticle delivery of a stable CRISPR-Cas9 RNP

Abstract Lipid nanoparticle (LNP) delivery of CRISPR ribonucleoproteins (RNPs) has the potential to enable high-efficiency in vivo genome editing with low toxicity and an easily manufactured technology, if RNP efficacy can be maintained during LNP production. In this study, we engineered a thermostable Cas9 from Geobacillus stearothermophilus (GeoCas9) using directed…

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Detection of Clostridium perfringens Using Novel Methods Based on Recombinase-Aided Amplification Assay-Assisted CRISPR/Cas12a System

Clostridium perfringens is a highly versatile pathogen of humans and animals. Rapid and sensitive detection methods for C. perfringens are urgently needed for the timely implementation of control. In this study, to provide novel promising methods for the detection of C. perfringens, two rapid, sensitive, and instrument-free C. perfringens detection…

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Rapid detection of avian influenza virus based on CRISPR-Cas12a | Virology Journal

Materials Escherichia coli strains expressing the LbCas12a protein, plasmids containing the AIV M and NP genes, and H1–H16 subtypes of AIV, newcastle disease virus (NDV), infectious bursal disease virus (IBDV), and infectious bronchitis virus (IBV) were conserved in the State Key Laboratory of Harbin Veterinary Research Institute (HVRI), Chinese Academy…

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Lung SORT LNPs enable precise homology-directed repair mediated CRISPR/Cas genome correction in cystic fibrosis models

Approximately 10% of Cystic Fibrosis (CF) patients, particularly those with CF transmembrane conductance regulator (CFTR) gene nonsense mutations, lack effective treatments. The potential of gene correction therapy through delivery of the CRISPR/Cas system to CF-relevant organs/cells is hindered by the lack of efficient genome editor delivery carriers. Herein, we report…

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Establishment of RT-RPA-Cas12a assay for rapid and sensitive detection of human rhinovirus B | BMC Microbiology

Clinical samples Thirty nasopharyngeal aspirates were collected from individuals with symptoms of respiratory tract infections, and tested positive for HRV-A (5 clinical samples), HRV-B (20 clinical samples), or HRV-C-positive (5 clinical samples) based on the method, as previously described [27]. Ten other specimens were tested negative for HRV-B, including human…

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Establishment of dsDNA-dsDNA interactions by the condensin complex.

journal contribution posted on 2023-11-09, 14:27 authored by Minzhe Tang, Georgii Pobegalov, Hideki Tanizawa, Zhuo A Chen, Juri Rappsilber, Maxim Molodtsov, Ken-ichi Noma, Frank Uhlmann Condensin is a structural maintenance of chromosomes (SMC) complex family member thought to build mitotic chromosomes by DNA loop extrusion. However, condensin variants unable to…

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Solved DNA viruses:Match the following characteristics for

DNA viruses:Match the following characteristics for the following DNA viruses. Only one answer foreach term and no answer is used more than once.TERMS POSSIBLE ANSWERS 1) Class I: dsDNA (+/-) virus A) Requires synthesis of DNA (-) before mRNA(+) can be transcribed. 2) Class II: ssDNA (+) virus. B) Can…

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Programmable all-DNA hydrogels based on rolling circle and multiprimed chain amplification products | APL Bioengineering

The ability to finely tune the properties of biocompatible hydrogels in a predictable and rational manner opens opportunities for the development of novel application-specific biomaterials for a wide range of advanced biomedical fields from soft robotics and drug delivery to biosensing and tissue engineering.1,2 Due to its innate programmability, DNA…

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Animals | Free Full-Text | RPA-CRISPR/Cas12a-Based Detection of Haemophilus parasuis

H. parasuis is a common pathogenic bacterium in the respiratory tract of pigs, which can cause widespread cellulose inflammation (also called Glässer’s disease) when the immunity of pigs is decreased. H. parasuis has fifteen serovars, and some clinical strains remain indistinguishable from serotypes. The vaccine’s cross-protection is poor among different…

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Amerigo Scientific Unveils CRISPR/Cas Enzymes to Expand

Amerigo Scientific, as a distributor focused on providing critical products and services to biomedical and life science communities, announced the release of a groundbreaking selection of CRISPR/Cas enzymes to its extensive life science portfolio, aimed at further expanding the vast potential of the CRISPR toolbox. This addition brings cutting-edge genetic…

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using the ssRNA genome as a template The ssDNA is then made into dsDNA which can

using the +ssRNA genome as a template. The ssDNA is then made into dsDNA, which can integrate into the host chromosome and become a permanent part of the host. The integrated viral genome is called aprovirus. The virus now can remain in the host for a long time to establish…

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Establishment of dsDNA-dsDNA interactions by the condensin complex

. 2023 Sep 27:S1097-2765(23)00746-3. doi: 10.1016/j.molcel.2023.09.019. Online ahead of print. Affiliations Expand Affiliations 1 Chromosome Segregation Laboratory, The Francis Crick Institute, London NW1 1AT, UK. 2 Mechanobiology and Biophysics Laboratory, The Francis Crick Institute, London NW1 1AT, UK; Department of Physics and Astronomy, University College London, London WC1E 6BT, UK….

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CRISPR-Cas12a test strip (CRISPR/CAST) package: In-situ detection of Brucella from infected livestock | BMC Veterinary Research

Infections caused by Brucella have emerged as a considerable threat worldwide, particularly in vast amounts of livestock. It is essential to eradicate and control Brucella infections in livestock from the source. In addition, earlier detection and timely culling are equally important. Consequently, the screening used for livestock must be accurate, sensitive, specific, simple,…

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A new method for the detection of Mycobacterium tuberculosis based on the CRISPR/Cas system | BMC Infectious Diseases

Expression and purification of Cas12a protein The pMBP-LbCas12a recombinant plasmid used in this study was purchased from Addgene Global Plasmid Information Sharing Platform (Plasmid#113,431) and was 9653 bp in size. The recombinant plasmid was transformed into DH5α competent state and cultured at 37℃ for 6 h. IPTG was added at a final…

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Foods | Free Full-Text | Qualitative and Quantitative Detection of CRISPR-Associated Cas Gene in Gene-Edited Foods

1. Introduction Gene editing technology can effectively edit target genes and is therefore a valuable tool in biological research [1,2,3]. The third-generation gene editing technology, namely clustered regularly interspaced short palindromic repeats (CRISPR), is a defense system in bacteria. The presence of these repeats was first discovered in Escherichia coli…

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PCR MCQ For NEET Biology With Answers

PCR MCQ Introduction PCR MCQ: Getting copies of a DNA or gene that we want to examine is the main goal of the polymerase chain reaction. We need to amplify DNA since some DNAs are difficult for us to see. The concept of temperature-dependent amplification emerged following the identification of thermostable…

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A CRISPR/Cas12a-SERS platform for amplification-free detection of African swine fever virus genes

African swine fever virus (ASFV) is a highly infectious and lethal agent in pigs and is classified as a “notifiable disease” by the World Organization for Animal Health (OIE) [1,2]. Although ASFV cannot infect humans, it is extremely difficult to prevent and control due to its multiple infection paths, which…

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Solved 3.35 A Predict the sequence of the product(s) that

Transcribed image text: 3.35 A Predict the sequence of the product(s) that would be formed if a DNA polymerase reaction were performed on the following singlestranded oligonucleotide (at a 1μmol scale) using DNA polymerase, excess primer, a mixture of dNTPs, and MigCl2​. ssDNA: 3′-ACTGGCCGTCGTTTTACAACGTACGTACGTACGTACGT ACGTACGTACGT −5′ Primer: 5′-TGACCGGCAGCAAAATGT- 3′ B…

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Detection of Salmonella DNA and drug-resistance mutation by PCR-based CRISPR-lbCas12a system | AMB Express

Establishment of PCR-lbCas12 DETECTR The whole detection process is illustrated in Fig. 1a. According to the data of infected persons from the Medical Laboratory of Shenzhen Luohu People’s Hospital, Salmonella is always among the top 5 positive Gram-negative (G−) bacilli in both 2021 and 2020 (Fig. 1b). To obtain efficient and specific…

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Application of CRISPR-Cas12a technology in zebrafish to simulate beta-globin gene (HBB) mutations

Abstract Background: The human beta-globin (HBB) gene translated into the hemoglobin subunit beta (beta) of the hemoglobin protein. When mutated, it can lead to the blood disorder beta-thalassemia. Recently, the CRISPR-Cas12 technology has shown promising potential in treating different genetic abnormalities. Objectives: This study aimed to evaluate the feasibility of…

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Can ssDNA oligonucleotides be combined and assembled with dsDNA fragments?

Whether you are performing your first cloning experiment or constructing multi-fragment DNA assemblies, NEB® has the solution for you. Our high quality reagents are available for every workflow, including popular DNA assembly methods such as NEBuilder® HiFi DNA Assembly and NEBridge® Golden Gate Assembly. We also offer solutions for automation, site-directed…

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Bacteriophage Market Report [2031]: Detailed Analysis

The Bacteriophage market research report for 2023 To 2031 | Segmented By Types [DsDNA Bacteriophage, SsDNA Bacteriophage, SsRNA Bacteriophage, Others] and Application [Animal Health, Aquaculture, Agriculture, Food Industry, Human Health, Others], This report is your definitive guide to understanding the dynamic landscape of this industry. It offers a comprehensive analysis of the market, providing essential insights into…

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Everyone is talking about arboviruses; but what exactly are they and what do they include?

September 17, 2023 it’s known arbovirus A variety of viruses transmitted to humans or other vertebrates by certain blood-sucking arthropods, esp. insect (flies and mosquitoes) and arachnids (check). In fact, arboviruses are not part of the current virus classification system, which is based on the nature and structure of their…

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“One-to-many” signal-output strategy-based CRISPR/Cas12a system for sensitive label-free fluorescence detection of HBV-DNA

Hepatitis B virus (HBV) associated nucleic acid (HBV-DNA) represents HBV replication [1]. Higher levels of HBV-DNA are strongly associated with cirrhosis and hepatocellular carcinoma development [2], [3], [4]. The early on-treatment of HBV-DNA levels are essential for assessing pathogen infection, disease progression, and future antiviral treatment planning [5], [6]. Current…

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Healthcare and Nursing Community

CRISPR-based diagnostics offer numerous advantages, shifting clinical testing from lab settings to a patient-centric approach. Yet, until now, tests have only targeted DNA. Here, we share how our lab developed SAHARA, a method for RNA detection. Over the last decade, molecular diagnostics have undergone a transformative revolution with the help…

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Conjugation of microbial-derived gold nanoparticles to different types of nucleic acids: evaluation of transfection efficiency

AuNP production Fusarium oxysporum was used for the extracellular production of AuNP. The fungus was acquired from the Culture Collection of Fungi of the Charles University (CCF 3732, Czech Republic). After cultivation in Sabouraud Dextrose Broth (SDB, Sigma Aldrich, Prague, Czech Republic) at 30 °C and 150 rpm for 4 days, the supernatant…

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Gene Editing Tools Based on CRISPR/Cas System and Their Improvement Strategies

     Gene Editing Tools Based on CRISPR/Cas System and Their Improvement Strategies XIN Yuan1,TIAN Kai-ren3,QIAO Jian-jun1,2,3,CAIYIN Qing-ge-le1,2,**() 1 School of…

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SU6.5 2022 notes – Lecture 6 introduces the concept of DNA sequencing, see Pierce pages 590 DNA

Lecture 6 introduces the concept of DNA sequencing, see Pierce pages 590 DNA sequencing is a powerful molecular method for analyzing DNA, as it allows you to quickly determine the sequence of bases in a DNA molecule. Analogous to some of the other technologies we have explored thus far, scientist…

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Scientists Develop Novel Base Editing System

Register for free to listen to this article Thank you. Listen to this article using the player above. ✖ Want to listen to this article for FREE? Complete the form below to unlock access to ALL audio articles. In a study published in Nature Biotechnology on Aug. 28, GAO…

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Novel based editors developed – Drug Target Review

Researchers have developed a novel based CRISPR-free base editing system named CyDENT, which enables precise base editing. In a study published in Nature Biotechnology, GAO Caixia’s team at the Institute of Genetics and Developmental Biology (IGDB) of the Chinese Academy of Sciences, in collaboration with researchers at Qi Biodesign, has introduced…

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Base Editors Show Strand-Specific Targeting in Nuclei and Organelles

Researchers from the Chinese Academy of Scientists (CAS) and their collaborators at Qi Biodesign have developed a strand-specific, CRISPR-free editing system for modifying genomes in the nucleus, mitochondria, and chloroplasts of plant and human cells. The team was led by Caixia Gao, PhD, a principal investigator at the Institute of…

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MArVD2: a machine learning enhanced tool to discriminate between archaeal and bacterial viruses in viral datasets

MArVD2 is a random forest classifier, implemented in the scikit-learn python package for novel archaeal virus discovery (Fig. 1) [61] where it’s trained and tested with separate datasets of archaeal viruses to best represent its performance in a variety of environments (Fig. 1). Integrating MArVD2 with machine learning introduces several practical and…

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A highly efficient scheme for library preparation from single-stranded DNA

Vector construction The gene encoding VTopoI (GenBank: L13447.1) was synthesized by Eurofins Genomics Inc (Tokyo, Japan), with codon optimization for Escherichia coli K-12, exclusion of internal BamHI and EcoRI sites, and their inclusion at the 5′- and 3′-ends of the DNA, respectively (Supplementary Sequences). The BamHI-EcoRI fragment of the gene…

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Counting dots or counting reads? Complementary approaches to estimate virus-to-microbe ratios

“How many viruses are there in the environment, compared to cells ?”. This deceptively simple question remains challenging to address today, yet because it relates to fundamental processes and characteristics of viral communities, even the imperfect approximations available have been critical for the field of viral ecology. Most notably, early…

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2023 Global Phage Therapy Market Emerging Trends and Opportunities: A Comprehensive Research Report

PRESS RELEASE Published August 15, 2023 “Final Report will add the analysis of the impact of COVID-19 on this industry.” The Global “Phage Therapy Market” Research, as presented in the 117 Pages report, provides comprehensive insights into the market based on types, applications, and geographical locations. These categories are further…

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GenScript Biotech and T-MAXIMUM Biotech Form Strategic Alliance on cGMP sgRNA

GenScript’s cGMP sgRNA reagent services enabled FDA-certified orphan drug designation (ODD) for T-MAXIMUM Biotech’s MT207 CAR-T cell therapy PISCATAWAY, N.J. and NANJING, China, Aug. 9, 2023 /PRNewswire/ — GenScript Biotech, the world’s leading life-science research tools and services provider, and T-MAXIMUM Biotech, a biotechnology company pioneering universal cell therapies, have…

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Additional file 1 of CRISPR/Cas12a combined with RPA for detection of T. gondii in mouse whole blood

journal contribution posted on 2023-07-31, 03:09 authored by Xiaofeng Wang, Miao Cheng, Shuqi Yang, Chen Xing, Qian Li, Yating Zhu, Yongsheng Ji, Yinan Du Additional file 1: Figure S1. Protein expression and purification. Coomassie blue-stained acrylamide gel. Figure S2. Specificity of the DETECTR reaction. The left tube represents B1-L3; the…

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CRISPR/Cas12a combined with RPA for detection of T. gondii in mouse whole blood | Parasites & Vectors

Mice and parasites The strains RH, Pru, wh3, and wh6, utilized in the present investigation, were generously provided by the Anhui Key Laboratory of Zoonoses, Anhui Medical University. The DNA of C. parvum and Babesia were procured from Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences. Furthermore, Plasmodium was…

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Pig manure treatment strategies for mitigating the spread of antibiotic resistance

Manure treatment The long-term goal of manure management is to remove environmental contaminants: disposing of the high volume of waste, using its value to improve soil fertility, and ensuring biosecurity. Hence, it was necessary to study the physicochemical properties of both treated and untreated manure. Composted pig manure has an…

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Self-primer exponential Cas12a tandem reaction-amplified and [Cu(tz)] nanosheets based neoteric biosensing platform for quantifying multiple mTBI-related miRNAs

Head injuries are a globally widespread problem, particularly among children and teenagers, who may be more susceptible to long-term sequelae during brain development. Concussion, a sign of mild traumatic brain injury (mTBI), affects an estimated 20% of teenagers [1], [2]. Unfortunately, due to delayed onset of symptoms and strong subjectivity…

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CPA-Cas12a-based lateral flow strip for portable assay of Methicillin-resistant Staphylococcus aureus in clinical sample | Journal of Nanobiotechnology

Principle of CPA-Cas 12a lateral flow assay strategy CPA and CPA-Cas 12a sensing strategies were proposed for detection of nuc and mecA genes in MRSA respectively. According to the sequence of the target gene, we designed two PAM sites and four pairs of primer according to the principles of double-crossing…

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Phenotypic and molecular basis of SIX1 variants linked to non-syndromic deafness and atypical branchio-otic syndrome in South Korea

Clinical phenotypes This study included eight affected patients (three males and five females) from five unrelated families, segregating with SIX1 variants (Fig. 1a). A comprehensive overview of their clinical phenotypes, encompassing both major and minor criteria, is provided in Table 1. All eight individuals (100%) experienced sensorineural hearing loss (SNHL), with…

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Development of an inducible Cas9 nickase and PAM-free Cas12a platform for bacterial diagnostics

. 2023 Jul 11;265:124931. doi: 10.1016/j.talanta.2023.124931. Online ahead of print. Affiliations Expand Affiliations 1 State Key Laboratory of Marine Resource Utilization in South China Sea, Marine College, Hainan University, Haikou 570228, China. 2 Agriculture and Agri-Food Canada, Fredericton, New Brunswick, E3B 4Z7, Canada; Nutra Health Products and Technologies Inc., Fredericton…

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cGMP CRISPR sgRNA and Non-viral DNA Payload Manufacturing Capacity Doubled

 GenScript Biotech Corporation, the world’s leading provider of life-science research tools and services, has expanded its cGMP manufacturing capacity for sgRNA and non-viral DNA payloads with a new, state-of-the-art facility in Zhenjiang, Jiangsu, China. This latest expansion provides biopharma companies and gene and cell therapeutic discovery startups a fast-turnaround source…

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Rapid and sensitive Cas12a-based one-step nucleic acid detection with ssDNA-modified crRNA,Analytica Chimica Acta

CRISPR-Cas12a RNA-guided complexes have been developed to facilitate the rapid and sensitive detection of nucleic acids. However, they are limited by the complexity of the operation, risk of carry-over contamination, and degradation of CRISPR RNA (crRNA). In this study, a Cas12a-based single-stranded DNA (ssDNA)-modified crRNA (mD-crRNA)-mediated one-step diagnostic method (CasDOS)…

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Transcription and FACT facilitate the restoration of replication-coupled chromatin assembly defects

Struhl, K. & Segal, E. Determinants of nucleosome positioning. Nat. Struct. Mol. Biol. 20, 267–273 (2013). Article  CAS  PubMed  PubMed Central  Google Scholar  Chereji, R. V. & Clark, D. J. Major determinants of nucleosome positioning. Biophys. J. 114, 2279–2289 (2018). Article  ADS  CAS  PubMed  PubMed Central  Google Scholar  Morgan, M….

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Researchers visualize activity of CRISPR genetic scissors in real time

Scheme and TEM images of the nanorotor. a, Three-dimensional scheme of the nanorotor including the origami rotor arm and a 50 nm AuNP. Blue cylinders represent the individual dsDNA helices. Insets at the top and bottom show enlarged perspectives as well as schematic views of the dsDNA ligation interfaces at each…

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Engineered bacterial orthogonal DNA replication system for continuous evolution

Arnold, F. H. Design by directed evolution. Acc. Chem. Res. 31, 125–131 (1998). Article  CAS  Google Scholar  Davis, A. M., Plowright, A. T. & Valeur, E. Directing evolution. The next revolution in drug discovery? Nat. Rev. Drug Discov. 16, 681–698 (2017). Article  CAS  PubMed  Google Scholar  Packer, M. S. &…

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Double-stranded DNA donor with novel structure boosts gene knock-in efficiency

Schematic of odsDNA production and 3 methods of gene knock-in. Credit: Prof. Liang’s group A research group led by Prof. Liang Haojun and researcher Bao Jianqiang from the University of Science and Technology of China (USTC) proposed a novel functional double-stranded DNA (dsDNA) donor that greatly enhances the efficiency of…

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Development of a Naked Eye CRISPR-Cas12a and -Cas13a Multiplex Point-of-Care Detection of Genetically Modified Swine

journal contribution posted on 2023-07-11, 14:06 authored by Yuan Wang, Lanting Fu, Dagang Tao, Xiaosong Han, Bingrong Xu, Manfei Deng, Sheng Li, Changzhi Zhao, Xinyun Li, Shuhong Zhao, Ping Gong, Yu Yang, Emmanuel Mulaya Khazalwa, Yunlong Ma, Jinxue Ruan, Changchun Li, Shengsong Xie The Rapid Visual CRISPR (RAVI-CRISPR) assay employs…

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GenScript doubles manufacturing capacity with new facility in China

To shorten the traditionally long wait times for crucial genetic material used to advance life-saving gene and cell therapies US-based GenScript Biotech Corporation has expanded its cGMP manufacturing capacity for sgRNA and non-viral DNA payloads with a new, state-of-the-art facility in Zhenjiang, Jiangsu, China. This latest expansion provides biopharma companies and gene and cell…

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GenScript Doubles cGMP sgRNA and Non-Viral DNA Payload Manufacturing Capacity

“As a researcher in the gene and cell therapy field, I have experienced firsthand the challenges of finding GMP-grade materials for editing cells. In our lab, we have been leveraging non-viral CRISPR and payload materials to efficiently reprogram various types of immune cells, due to multiple advantages non-viral materials possess,…

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GenScript Doubles CGMP SgRNA Manufacturing Capacity

GenScript Biotech Corporation, a provider of life-science research tools and services, has expanded its cGMP manufacturing capacity for sgRNA and non-viral DNA payloads with a new, state-of-the-art facility in Zhenjiang, Jiangsu, China. The latest expansion provides biopharma companies and gene and cell therapeutic discovery startups with components needed to advance…

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06.07.2023 | Press Release | GenScript doubles production capacity for cGMP sgRNA and non-viral DNA payload

Additional 400,000 square feet at GenScript’s Zhenjiang cGMP facility, China provides biopharmaceutical companies and gene/cell therapy research startups with fast access to synthetic CRISPR sgRNA and non-viral single and double-stranded DNA payloads PISCATAWAY, NJ, July 6, 2023 /PRNewswire/ — GenScript Biotech Corporation, the global leader in life science research tools…

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Converting ssDNA oligos to dsDNA with T4 DNA polymerase

This protocol allows one to convert ssDNA to dsDNA oligos. In principle, one can buy two complementary oligos and anneal them. However, there are a few cases where randomized bases are desired, and therefore complementary oligos can not be ordered. The protocol works similarly to PCR, except that the reaction…

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Postdoctoral Researcher in Plant Biotechnology and Genome Editing

Category Research / Academic Location Amsterdam Crispr/Cas technology has revolutionized plant biotechnology by making targeted genetic modifications possible. Are you fascinated by the seemingly unlimited potential of this technology, and do you want to contribute to its further development and apply it to broaden plant immunity? Then this may be…

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CasDinG is a 5′-3′ dsDNA and RNA/DNA helicase with three accessory domains essential for type IV CRISPR immunity

doi: 10.1093/nar/gkad546. Online ahead of print. Affiliations Expand Affiliations 1 Department of Chemistry and Biochemistry, Utah State University, Logan, UT, USA. 2 Center for Integrated Biosystems, Utah State University, Logan, UT, USA. Item in Clipboard Hannah Domgaard et al. Nucleic Acids Res. 2023. Show details Display options Display options Format AbstractPubMedPMID…

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A self-supply crRNA-mediated CRISPR/Cas12a-driven controlled-release homogeneous biosensor for ultrasensitive detection of microRNA

Abnormal expression of microRNAs (miRNAs) will lead to various diseases [1], [2], [3], [4], [5], including cancer, therefore, the ultrasensitive detection of miRNAs is critical for the early cancer diagnosis and therapy. Recently, some analytical methods, such as electrochemistry, Raman, and fluorescence have been widely used to detect miRNAs [6],…

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Apolipoprotein E genetic analysis in unamplified genomic DNA extracts by ligase reaction and fiber optic particle plasmon resonance biosensor

Alzheimer’s disease is the most common type of dementia in elderly people, accounting for over 60% of all confirmed cases [1]. The two strongest risk factors for AD are age followed by apolipoprotein E (APOE) genotype [2]. The APOE gene has three major alleles defined by the combination of two…

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Branched DNA switchable CRISPR-Cas12a system for sensing FEN1 activity

The discovery of Flap endonuclease 1 (FEN1) can be traced back to the 1960 s of the last centuries, Klett et al. discovered “Exonuclease VI” while studying Escherichia coli [1]. FEN1 is a structure-specific nuclease that precisely recognizes a double-flap DNA structure consisting of unpaired 5′-overhang and a single nucleotide 3′-flap,…

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Researchers develop new base editing tools us

image: AI-assisted structural predictions and alignments establishes a new protein classification and functional mining method, further enabling the discovery of a suite of single and double-strand cytidine deaminases which show great potential as bespoke base editors for therapeutic or agricultural breeding applications. view more  Credit: IGDB GAO Caixia’s group from the…

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Global Analysis and Forecast By 2030

PRESS RELEASE Published June 25, 2023 Phage Therapy Market Report Presents An Wide Estimation Of Industry Competitors, Including [ CJ Cheiljedang, Pherecydes Pharma, VetoPhage, Cytophage, Locus Biosciences, MicroMir, Eliava BioPreparations, CTC Bio, INtODEWORLD, Phagelux, EasyBio, Intralytix, Omnilytics, NPO Microgen, Micreos, Proteon Pharmaceuticals ]. Phage Therapy market size was valued at…

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Type IV-A CRISPR-Csf complex: Assembly, dsDNA targeting, and CasDinG recruitment

. 2023 Jun 13;S1097-2765(23)00420-3. doi: 10.1016/j.molcel.2023.05.036. Online ahead of print. Affiliations Expand Affiliations 1 Department of Biochemistry, School of Medicine, Southern University of Science and Technology, Shenzhen 518055, China. 2 Key Laboratory of Molecular Design for Plant Cell Factory of Guangdong Higher Education Institutes, Department of Chemical Biology, School of…

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Population ecology and potential biogeochemical impacts of ssDNA and dsDNA soil viruses along a permafrost thaw gradient

Abstract Climate change is disproportionately warming northern peatlands, which may release large carbon stores via increased microbial activity. While there are many unknowns about such microbial responses, virus roles are especially poorly characterized with studies to date largely restricted to bycatch from bulk metagenomes. Here, we used optimized viral particle…

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Bacteriophage Market Competition Situation 2023| NPO Microgen, Proteon Pharmaceuticals, Phagelux, Intralytix

PRESS RELEASE Published June 13, 2023 Bacteriophage Market has been updated by MarketQuest.biz comprises of a comprehensive investigation into the geographical landscape, industry size along with the revenue estimation of the business. The report provides a comprehensive study on growth drivers and notable trends impacting the future growth of the…

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Revolving hexameric ATPases as asymmetric motors to translocate double-stranded DNA genome along one strand

Review . 2023 May 19;26(6):106922. doi: 10.1016/j.isci.2023.106922. eCollection 2023 Jun 16. Affiliations Expand Affiliations 1 Center for RNA Nanobiotechnology and Nanomedicine, The Ohio State University, Columbus, OH, USA. 2 College of Pharmacy, Division of Pharmaceutics and Pharmacology, The Ohio State University, Columbus, OH, USA. 3 College of Medicine, Dorothy M….

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Regulation of the CRISPR-Cas12a system by methylation and demethylation of guide RNA

. 2023 May 9;14(22):5945-5955. doi: 10.1039/d3sc00629h. eCollection 2023 Jun 7. Affiliations Expand Affiliations 1 Beijing Key Laboratory for Bioengineering and Sensing Technology, School of Chemistry and Biological Engineering, University of Science and Technology Beijing 30 Xueyuan Road, Haidian District Beijing 100083 P. R. China. 2 Department of Chemistry, Tsinghua University…

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I-Mitochondrial DNA (mtDNA) Indlela Yokuphindaphinda, Izinto

Ikhaya » Microbiology » Ufuzo Advertisements HSP umgqugquzeli we-strand esindayo I-LSP ukukhanya strand umgqugquzeli MGME1 i-mitochondrial genome maintenance exonuclease 1 mtSSB I-mitochondrial enemicu eyodwa ye-DNA-binding protein NCR isifunda esingabhali ikhodi I-TAS ukulandelana okuhlobene nokuqedwa I-Mitochondrial DNA (mtDNA) iyi-molecule eyi-16.6 kilobase enemicu ephindwe kabili. Umucu owodwa we-DNA ye-mitochondrial inothe ngama-guanines, okuvumela…

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Phage-Based Treatment Market to witness Astonishing Growth, Observe latest Development and Precise Outlook 2023-2030NPO Microgen, Proteon Pharmaceuticals

The Phage-Based Treatment Market, where demand is steadily increasing due to improved purchasing power, is expected to be beneficial for the global market. This comprehensive research report is an exhaustive compilation of market developments and growth factors optimizing their future growth trajectories from accurate data on the products, strategies, and…

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Crispr/Cas12a Collateral Cleavage-Driven Transcription Amplification for Direct Nucleic Acid Detection by Ha-Yeong Lee, Deok-Gyu Lee, Kyung-Ho Lee, Juyoung Byun, Yong-Beom Shin :: SSRN

21 Pages Posted: 31 May 2023 See all articles by Ha-Yeong Lee Korean Research Institute of Bioscience and Biotechnology Korean Research Institute of Bioscience and Biotechnology Apteasy MJ Inc Korean Research Institute of Bioscience and Biotechnology University of Science and Technology(UST)…

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Phage Therapy Market Size, Share New Report [2023-2030]

PRESS RELEASE Published May 29, 2023 . Phage Therapy Market (2023) Report offers an in-depth analysis of the market status of the Phage Therapy Top manufacturers with definition, SWOT analysis, best facts and figures, PESTAL analysis, expert opinions, and the most recent developments worldwide. Phage Therapy Market Size, Share, Growth,…

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ssDNA is not superior to dsDNA as long HDR donors for CRISPR-mediated endogenous gene tagging in human diploid RPE1 and HCT116 cells | BMC Genomics

An optimized CRISPR knock-in method using long dsDNA donors for efficient tagging with fluorescent proteins in human diploid cells. Given the widespread use of CRISPR-mediated generation of knock-in cell lines, it is informative to compare the performance of long dsDNA and ssDNA donors in a simple and practical knock-in method….

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Point-of-care detection of Neisseria gonorrhoeae based on RPA-CRISPR/Cas12a | AMB Express

Reagents Primers for RPA were synthesized by TsingKe Biological Technology (Beijing, China). Recombinant RNase inhibitor, crRNA, and RNase-free water were obtained from Takara Bio (Beijing, China). The ssDNA reporter was synthesized by Sangon Biotech (Shanghai, China). The TIANamp Bacteria DNA kit was purchased from TIANGEN (Beijing, China). The TwistAmp Basic…

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Allosteric probe-triggered isothermal amplification to activate CRISPR/Cas12a for sensitive electrochemiluminescence detection of Salmonella,Food Chemistry

We report an electrochemiluminescence (ECL) sensor for Salmonella detection based on allosteric probe as a bio-recognition element and CRISPR/Cas12a as a signal amplification strategy. In the presence of Salmonella, the structure switching occurs on allosteric probes, resulting in their hybridization with primers to trigger isothermal amplification. Salmonella is then released…

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Allosteric probe-triggered isothermal amplification to activate CRISPR/Cas12a for sensitive electrochemiluminescence detection of Salmonella

Food-borne pathogens can cause a variety of diseases and seriously threaten human health (Pires et al., 2021). Among all food-borne pathogens, Salmonella is a ubiquitous and fatal bacterial species. It is known that Salmonella is a Gram-negative facultative anaerobe and one of the microorganisms with drug-resistant serotypes (Tanner,& Kingsley, 2018)….

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USTC has made new progress in the optimization of gene knock-in donors

Professor Liang Haojun, Hefei National Research Center for Physical Sciences at the Microscale, School of Chemistry and Materials Science, University of Science and Technology of China, cooperated with researcher Bao Qiangqiang from the Department of Life Sciences and Medicine, and optimized and proposed the current gene knock-in donors in terms…

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A cathodic photoelectrochemical biosensor based on CRISPR/Cas12a trans-cleavage mediated p-n heterojunction quenching mode for microRNA determination

MicroRNAs (miRNA) are a group of endogenous nucleotide molecules with short single chains that are less susceptible to genetic mutations. Abnormal expression of miRNAs is closely associated with the occurrence of cancer [[1], [2], [3], [4]]. MiRNA-122, serving as a key biomarker for the early diagnosis of liver cancer, is…

Continue Reading A cathodic photoelectrochemical biosensor based on CRISPR/Cas12a trans-cleavage mediated p-n heterojunction quenching mode for microRNA determination

3D dynamic DNA walker traveling on DNA micelles coupled CRISPR-Cas12a nucleases to recover electrochemiluminescence signal for microcystin-LR detection

DNA walkers are molecular self-assemblers designed according to Watson-Crick base complementary pairing rules that convert chemical energy into precise mechanical motion on the nanoscale in response to external stimuli[1], [2], [3], [4]. The autonomous movement and repetitive stepping characteristics bestow exceptional signal-amplification potential for applications in molecular computing, biosensing, and…

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Quantitative DNA-RNA Immunoprecipitation Sequencing with Spike-Ins – PubMed DNA:RNA ImmunoPrecipitation and high-throughput sequencing.

doi: 10.1007/978-1-0716-2477-7_26. Memberships Expand Connections 1 Department about Chemical and Systems Biology, Stanford University, Stand-ford, CANCER, USA. [email protected] 2 Department of Chemical and Systems Nature, Stanford University, Stanford, CA, USA. [email protected] Freely PMC article Item in Clipboard Magdalena P Crossley et aluminium. Methods Mol Bil. 2022. Free PMC books Show details…

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Fluorescence and Colorimetric Analysis of African Swine Fever Virus Based on the RPA-Assisted CRISPR/Cas12a Strategy

It is well-established that different detection modes are necessary for corresponding applications, which can effectively reduce matrix interference and improve the detection accuracy. Here, we reported a magnetic separation method based on recombinase polymerase amplification (RPA)-assisted clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a for dual-mode analysis of African swine fever…

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Highly sensitive and facile microRNA detection based on target triggered exponential rolling-circle amplification coupling with CRISPR/Cas12a

MicroRNAs (miRNAs) are small noncoding RNAs consisting of approximately 20–25 nucleotides. As a kind of transcription regulation factors, miRNAs perform post-transcriptional repression by binding to the 3′UTR of target in the seed region, which is considered as a typical mode of miRNA-mediated gene regulation [[1], [2], [3]]. And they play…

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A one-pot isothermal Cas12-based assay for the sensitive detection of microRNAs

Mechanistic studies of the EXTRA-CRISPR assay The assay is designed to be a tri-enzymatic cascade that exploits the unique nucleolytic cleavage activities of the CRISPR–Cas12a system to create a new exponential isothermal amplification mechanism based on the robust linear RCA assay. The assay starts with the hybridization of a padlock…

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CRISPR-Cas12a Coupled with DNA Nanosheet-Amplified Fluorescence Anisotropy for Sensitive Detection of Biomolecules

journal contribution posted on 2023-04-25, 19:35 authored by Tian Jin Xie, Jia Li Xie, Yu Jie Luo, Kai Mao, Cheng Zhi Huang, Yuan Fang Li, Shu Jun Zhen DNA nanosheets (DNSs) have been utilized effectively as a fluorescence anisotropy (FA) amplifier for biosensing. But, their sensitivity needs to be further…

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From 2023 to 2031 Phage Therapy Market 2023 [ SIZE and TREND ]

PRESS RELEASE Published April 24, 2023 “Phage Therapy Market“ProductApplication CoverageMarket Size and Forecast, Different Demand Market by Region, Main Consumer Profile [Animal Health, Aquaculture, Agriculture, Food Industry, Human Health, Others],Product Type CoverageMarket Size and Forecast-2031, Major Company of Product Type [DsDNA Bacteriophage, SsDNA Bacteriophage, SsRNA Bacteriophage, Others]. An Extensive Report…

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Peptide-mediated delivery of CRISPR enzymes for the efficient editing of primary human lymphocytes

Weber, E. W., Maus, M. V. & Mackall, C. L. The emerging landscape of immune cell therapies. Cell 181, 46–62 (2020). CAS  PubMed  PubMed Central  Google Scholar  Eyquem, J. et al. Targeting a CAR to the TRAC locus with CRISPR/Cas9 enhances tumour rejection. Nature 543, 113–117 (2017). CAS  PubMed  PubMed…

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Unveiling a fast and efficient method for det

image: After hybridizing to the precircularized circular probe, the target miRNA is amplified into a long ssDNA product by RCA with massive trigger sequences repeats. The trigger sequence is designed as the activator of Cas12a for showing trans-cleavage activity. Then, the trigger chain is recognized by Cas12a–crRNA duplex and hybridizes to…

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