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Tag: ssODNs
CRISPR/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane
Introduction Cystic fibrosis (CF) is a lethal, autosomal recessive disorder caused by mutations in the CFTR gene that results in aberrant transport of chloride, bicarbonate, and sodium ions (Kerem et al, 1989; Riordan et al, 1989; Rommens et al, 1989; Tabcharani et al, 1991; Poulsen et al, 1994; Stutts et…
A duplex tetra-primer ARMS-PCR assay to discriminate three species of the Schistosoma haematobium group: Schistosoma curassoni, S. bovis, S. haematobium and their hybrids | Parasites & Vectors
Gray IC, Campbell DA, Spurr NK. Single nucleotide polymorphisms as tools in human genetics. Hum Mol Genet. 2000;9:2403–8. Article CAS PubMed Google Scholar Shastry BS. SNP alleles in human disease and evolution. J Hum Genet. 2002;47:561–6. Article CAS PubMed Google Scholar Rocha D, Gut I, Jeffreys AJ, Kwok P-Y, Brookes…
New lipid nanoparticle CRISPR delivery system developed
Lipid nanoparticles have been used to encapsulate CRISPR-Cas9 and deliver it to cells in mice, where it was highly effective at knocking down expression of a target protein. Gene therapy is a potential mode of treatment for a wide variety of diseases caused by genetic mutations. While it has been…
Scientists introduced alligator DNA into catfish to create hybrid
The use of CRISPR also casts doubt on the viability of the technique as it may prove “too difficult to produce enough of these fish to get a viable, genetically healthy line going,” says Greg Lutz, an expert on aquaculture genetics at Louisiana State University. There is also uncertainty surrounding…