Tag: ssRNA

Development and clinical evaluation of a CRISPR/Cas13a-based diagnostic test to detect Mycobacterium tuberculosis in clinical specimens

Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis (MTB) complex, remains one of the leading infectious causes of death worldwide, with an estimated 1.6 million TB deaths in 2021 (World Health Organization, 2022). Rapid and accurate TB diagnosis is critical to ensure timely initiation of anti-TB therapy (Walzl et al., 2018)….

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Cryo-EM structure and protease activity of the type III-E CRISPR-Cas effector

Hampton, H. G., Watson, B. N. J. & Fineran, P. C. The arms race between bacteria and their phage foes. Nature 577, 327–336 (2020). Article  CAS  Google Scholar  Makarova, K. S. et al. Evolution and classification of the CRISPR-Cas systems. Nat. Rev. Microbiol. 9, 467–477 (2011). Article  CAS  Google Scholar …

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Origins and diversification of animal innate immune responses against viral infections

Harvey, E. & Holmes, E. C. Diversity and evolution of the animal virome. Nat. Rev. Microbiol. 20, 321–334 (2022). Article  CAS  Google Scholar  Flajnik, M. F. & du Pasquier, L. Evolution of innate and adaptive immunity: can we draw a line? Trends Immunol. 25, 640–644 (2004). Article  CAS  Google Scholar …

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Solved Which of the following statements about PCR is FALSE?

Transcribed image text: Which of the following statements about PCR is FALSE? Check all that apply. PCR uses a heat-stable DNA polymerase. After every cycle of replication, there is a linear increase in the amount of DNA present. Every round of replication starts with the denaturation of dsDNA molecules. If…

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Metagenomic analysis of viromes in tissues of wild Qinghai vole from the eastern Tibetan Plateau

Overview of the viromes In all, 41 wild Qinghai voles were collected from pasture habitats located on the eastern Tibetan Plateau, China (Fig. 1). Tissue samples from liver, lung, spleen, small intestine (with content), and feces (large intestinal content) of each vole were disrupted, and viral RNA was extracted. The RNA…

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Phage Therapy market 2022 extent USD 296.62% billion by 2027 | expected to grow at a compound annual growth rate of 10.91% | no of page 126

In the “Phage Therapy” Market Size 2022 | No. of Pages: 126 Furthermore, research categorizes the global Phage Therapy market by top players/brands, region, type and end user, including driving elements, Upstream Markets, and the general market circumstance. On the basis of product type this (DsDNA Bacteriophage, SsDNA Bacteriophage, SsRNA…

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Solved Put the steps of HIV replication in the correct

Put the steps of HIV replication in the correct order. Below is a sequence of events. Place them in the order they should occur, number 1 being the first item. Select the step number from the drop down next to each item. Items to order: 1. DNA polymerase makes the…

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RCSB PDB – 7SBA: Structure of type I-D Cascade bound to a dsDNA target

CRISPR-Cas systems are adaptive immune systems that protect prokaryotes from foreign nucleic acids, such as bacteriophages. Two of the most prevalent CRISPR-Cas systems include type I and type III. Interestingly, the type I-D interference proteins contain characteristic features of both type I and type III systems … CRISPR-Cas systems are…

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CASCADE: Naked eye-detection of SARS-CoV-2 using Cas13a and gold nanoparticles

This article was originally published here Anal Chim Acta. 2022 May 1;1205:339749. doi: 10.1016/j.aca.2022.339749. Epub 2022 Mar 22. ABSTRACT The COVID-19 pandemic has brought to light the need for fast and sensitive detection methods to prevent the spread of pathogens. The scientific community is making a great effort to design…

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Virology: Module 2 Flashcards | Quizlet

Virology: Module 2 Flashcards | Quizlet   attachment/entry, translation of viral mRNA into viral proteins, replication, assembly, release of viruses from cell steps of infection for viruses? a cell possesses the functional receptor for a virus – virus can bind, possibly enter, but replication isn’t guaranteed a cell does not…

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