Tag: stem

circRNA: Regulatory factors and potential therapeutic targets in inflammatory dermatoses

Review doi: 10.1111/jcmm.17473. Online ahead of print. Affiliations Expand Affiliations 1 Shanxi Key Laboratory of Stem Cells for Immunological Dermatosis, Institute of Dermatology, Taiyuan Central Hospital of Shanxi Medical University, Taiyuan, China. 2 Huashan Hospital, Shanghai Medical College, Fudan University, Shanghai, China. Item in Clipboard Review Ruifeng Liu et al. J…

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This startup wants you to have a personal stem cell stash

Salvatore Viscomi always wanted to be involved in a science fiction project — and he found one. Viscomi is the chief medical officer of GoodCell, a Massachusetts-based startup where people can bank their own white blood cells with a simple blood draw. Labs can then tweak those cells to become…

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Induzierte pluripotente Stammzellen (IPSCs) Marktanteil, Analyse der wichtigsten Wettbewerber, Prognose bis 2028

Der Induzierte pluripotente Stammzellen (IPSCs) Market“-Forschungsbericht umfasst umfassende Daten zu vorherrschenden Trends, Treibern, Wachstumschancen und Einschränkungen, die die marktverändernden Aspekte der globalen Industrie variieren können. Dieser Bericht bietet eine eingehende Analyse der Marktsegmentierung, die Produkte, Anwendungen und geografische Analysen enthält. Der globale Induzierte pluripotente Stammzellen (IPSCs)-Marktbericht bietet eine genaue Beobachtung…

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Single-cell analyses define a continuum of cell state and composition changes in the malignant transformation of polyps to colorectal cancer

Mapping molecular changes across malignant transformation We generated single-cell data for 81 samples collected from eight FAP and seven non-FAP donors (Fig. 1a and Supplementary Tables 1 and 2). For each tissue, we performed matched scATAC-seq and snRNA-seq (10x Genomics). We obtained high-quality single-cell chromatin accessibility profiles for 447,829 cells…

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Faculty Opinions: hiPSCs for predictive modelling of neurodegenerative diseases: dreaming the possible.

Rivetti di Val Cervo P et al. Nature Reviews. Neurology. 2021 06; 17(6):381-392 doi.org/10.1038/s41582-021-00465-0PMID: 33658662 Show Details AbstractAuthors Human induced pluripotent stem cells (hiPSCs) were first generated in 2007, but the full translational potential of this valuable tool has yet to be realized. The potential applications of hiPSCs are especially…

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Tumorigenicity risk of iPSCs in vivo: nip it in the bud

Review . 2022 Feb 3;5(1):pbac004. doi: 10.1093/pcmedi/pbac004. eCollection 2022 Mar. Affiliations Expand Affiliations 1 Department of Cell Biology, Naval Medical University, Shanghai 200433, China. 2 Department of Biochemistry and Molecular Biology, School of Basic Medical Sciences, and Guangdong Provincial Key Laboratory of Single Cell Technology and Application, Southern Medical University,…

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Biotechnology Careers

Images for Biotechnology Careers Biotechnology Careers: In-Demand Jobs Shaping Our Future ICAR-NRCB Recruitment 2020 – BSc Microbiology & Biotechnology Job Govt RGCB Research Jobs – Biotechnology & Bioinformatics JRF Position Biotech Jobs | CT19 Biotechnology Job Outlook | A Career in High Demand 9 of the Best Biotechnology Careers For…

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A desirable transgenic strategy using GGTA1 endogenous promoter-mediated knock-in for xenotransplantation model

Lai, L. et al. Production of alpha-1,3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science 295, 1089–1092. doi.org/10.1126/science.1068228 (2002). ADS  CAS  Article  PubMed  Google Scholar  Kuwaki, K. et al. Heart transplantation in baboons using alpha1,3-galactosyltransferase gene-knockout pigs as donors: initial experience. Nat. Med. 11, 29–31. doi.org/10.1038/nm1171 (2005). CAS  Article  PubMed  Google…

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SMARCE1 deficiency generates a targetable mSWI/SNF dependency in clear cell meningioma

Clapier, C. R., Iwasa, J., Cairns, B. R. & Peterson, C. L. Mechanisms of action and regulation of ATP-dependent chromatin-remodelling complexes. Nat. Rev. Mol. Cell Biol. 18, 407–422 (2017). CAS  PubMed  PubMed Central  Article  Google Scholar  Mashtalir, N. et al. Modular organization and assembly of SWI/SNF family chromatin remodeling complexes….

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Seven Bridges, Brazilian Researchers Applying Graph Analysis to Build Diverse Reference Genome

CHICAGO – The latest of Seven Bridges Genomics’ efforts to diversify reference genomes is its largest and perhaps most complex to date, an attempt to address the Brazilian population. The Charlestown, Massachusetts-based bioinformatics company recently joined with the University of São Paulo (USP), the Associação Genomas Brasil (Brazil Genome Association),…

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Impact of DNA mutations on lifelong blood cell production uncovered — ScienceDaily

New research has uncovered how genetic mutations hijack the production of blood cells in different periods of life. Scientists at the Wellcome Sanger Institute, the Cambridge Stem Cell Institute, EMBL’s European Bioinformatics Institute (EMBL-EBI) and collaborators show how these changes relate to ageing and the development of age-related diseases, including…

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Roles of circRNAs | CMAR

Introduction Hepatocellular carcinoma (HCC) is the most common type of primary liver cancer, with an increasing global incidence, and a leading cause of cancer-related mortality.1 Although hepatitis B and C virus infections, cirrhosis, alcohol intake, and non-alcoholic fatty liver disease (NAFLD) are the main risk factors contributing to HCC.1,2 Approximately…

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LncRNA Sequencing Helps to Find Deep Links to Disease

Long non-coding RNAs (lncRNAs) are a class of RNAs that do not code for proteins and are typically greater than 200 nucleotides in length. Studies have shown that lncRNAs are quite complex in function and play an important role in various biological processes such as development, differentiation, proliferation, metastasis, apoptosis,…

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Roche (RHHBY) Gets EC Approval for Polivy Combination in DLBCL – May 26, 2022

Roche (RHHBY Quick QuoteRHHBY – Free Report) recently announced that the European Commission (“EC”) has granted approval to lymphoma drug Polivy (polatuzumab vedotin) in combination with MabThera (rituximab) plus cyclophosphamide, doxorubicin and prednisone (R-CHP) for the treatment of adult patients with previously untreated diffuse large B-cell lymphoma (DLBCL). Polivy is…

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Allele-specific H3K9me3 and DNA methylation co-marked CpG-rich regions serve as potential imprinting control regions in pre-implantation embryo

. 2022 May;24(5):783-792. doi: 10.1038/s41556-022-00900-4. Epub 2022 Apr 28. Affiliations Expand Affiliations 1 Institute for Regenerative Medicine, Shanghai East Hospital, Shanghai Key Laboratory of Signaling and Disease Research, Frontier Science Center for Stem Cell Research, School of Life Sciences and Technology, Tongji University, Shanghai, China. 2 Shanghai Institute of Stem…

Continue Reading Allele-specific H3K9me3 and DNA methylation co-marked CpG-rich regions serve as potential imprinting control regions in pre-implantation embryo

Neural Stem Cell Reactivation in Cultured Drosophila Brain Explants

A method to reactivate quiescent neural stem cells in cultured Drosophila brain explants has been established. Using this method, the role of systemic signals can be uncoupled from tissue-intrinsic signals in the regulation of neural stem cell quiescence, entry and exit. We developed a method to reactivate quiescent neural stem…

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Recombinant Feline EGF Protein, HEK293 Cells, hFc Tag, 62010-U01H

EGF Protein, Feline, Recombinant (ECD, hFc Tag): 产品信息 纯度 > 90 % as determined by SDS-PAGE. 生物活性 Testing in progress 蛋白构建 A DNA sequence encoding the Feline EGF (NP_001009381.1) (Asn969-Arg1020) was expressed with the fused Fc region of human IgG1 at the N-terminus. 表达宿主 HEK293 Cells 分子量 The…

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CENTURY THERAPEUTICS, INC. Management’s discussion and analysis of financial condition and results of operations (form 10-Q)

The following discussion and analysis should be read in conjunction with our financial statements and accompanying notes included in this Quarterly Report on Form 10-Q and the financial statements and accompanying notes thereto for the fiscal year ended December 31, 2021 and the related Management’s Discussion and Analysis of Financial…

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Single-cell eQTL models reveal dynamic T cell state dependence of disease loci – Nature – Alert Breaking News

Wei, K. et al. Notch signalling drives synovial fibroblast identity and arthritis pathology. Nature 582, 259–264 (2020). ADS CAS PubMed PubMed Central Article Google Scholar  Cembrowski, M. S. & Menon, V. Continuous variation within cell types of the nervous system. Trends Neurosci. 41, 337–348 (2018). CAS PubMed Article Google Scholar  Maurano, M. T. et al. Systematic localization of…

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Single-Cell Transcriptome Resource From Human Cell Atlas Team Yields Immune, Disease Insights

NEW YORK – A large international team came up with single-cell and single-nucleus transcriptomic resources to characterize the diverse cell types found in the human body, along with related gene expression and splicing features that offer clues to processes at play within and across tissues and organs. In a series…

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Special Issue : Methods and Tools in RNA Biology

A special issue of Non-Coding RNA (ISSN 2311-553X). Deadline for manuscript submissions: 30 November 2022 | Viewed by 9 Special Issue Editors Dr. Mirolyuba Ilieva E-Mail Website Guest Editor Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, DK-2450 Copenhagen SV, DenmarkInterests: non-coding RNAs; stem cell biology; neuroscience Special…

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CALITHERA BIOSCIENCES, INC. MANAGEMENT’S DISCUSSION AND ANALYSIS OF FINANCIAL CONDITION AND RESULTS OF OPERATIONS (form 10-Q)

You should read the following discussion and analysis of our financial condition and results of operations in conjunction with our unaudited condensed consolidated financial statements and related notes included in Part I, Item 1 of this report. This Quarterly Report on Form 10-Q contains forward-looking statements within the meaning of…

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Our genetic strength in numbers

Although it’s 20 years since the first human genome was sequenced – that is, the entire collection of our DNA – our ability to ‘read’ our own instruction manual is no less astounding. We now know we have around three billion chemical base pairs that provide the code for approximately…

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Bioinformatics Jobs in Lausanne, Vaud

3.1 Data Manager Our client will be looking for a candidate with Data Management experience and experience in a previous Data Manager role.… 3.8 4.3 4.3 PhD Student in Gene Regulation There are regular possibilities to present and participate in local or international conferences and workshops. A copy of your…

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Global CRISPR/Cas9 Market In- Depth Research, Industry Statistics 2022

Global “CRISPR/Cas9 Market” Reports 2022 provide key industry studies for CRISPR/Cas9 manufacturers with specific statistics, significance, definition, SWOT analysis, expert opinion and the latest developments in the world. The research report also covers market size, price, sales, revenue, market shares, gross margin, growth rate and cost structure. The report aims…

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ISPE announces 2022 Facility of the Year Awards winners

Winners of ISPE’s Facility of the Year Awards (FOYA) 2022 include Janssen, Takeda, CRISPR Therapeutics, Catalent and Iovance Biotherapeutics. ISPE has announced the 2022 Facility of the Year Awards (FOYA) Category Winners at the 2022 ISPE Europe Annual Conference in Madrid, Spain.  FOYA is the global awards programme recognising innovation and creativity…

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Anthony Nolan hiring Bioinformatics Developer in Swiss Cottage, England, United Kingdom

We’re Anthony Nolan and we save the lives of people with blood cancer who need a stem cell transplant. We’re unique. Thanks to the incredible donors on our register, our pioneering research and our passionate fundraisers, we give the chance of life to three people a day. But we won’t…

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Identifying Mechanisms of Methylation in Stem Cells

Normal cells (red) survive much longer than Set1A-mutant cells (green) when ING5 is deleted. Northwestern Medicine scientists have identified critical regulatory processes that govern differentiation in embryonic stem cells, according to a study published in the Proceedings of the National Academy of the Sciences (PNAS). Bercin Cenik, MD, PhD, postdoctoral…

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Technical Officer / Micro-injectionist job with EMBL

The European Molecular Biology Laboratory (EMBL) is one of the highest-ranked scientific research organisations in the world. The Headquarters Laboratory is located in Heidelberg (Germany), with additional sites in Barcelona (Spain), Grenoble (France), Hamburg (Germany), Hinxton (UK), and Rome (Italy). The EMBL Epigenetics and Neurobiology Unit in Rome is supported…

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Electromagnetic Navigation Transthoracic Nodule Localization for Minimally Invasive Thoracic Surgery

Presented here is a protocol for lung nodule localization using dye marking via electromagnetically navigated transthoracic needle access. The technique described here can be accomplished in the peri-operative period to optimize nodule localization and to successful resection when performing minimally invasive thoracic surgery. The main advantage of this technique is…

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Landscape of helper and regulatory antitumour CD4+ T cells in melanoma

Sallusto, F. & Lanzavecchia, A. Heterogeneity of CD4+ memory T cells: functional modules for tailored immunity. Eur. J. Immunol. 39, 2076–2082 (2009). CAS  PubMed  Article  Google Scholar  Swain, S. L., McKinstry, K. K. & Strutt, T. M. Expanding roles for CD4+ T cells in immunity to viruses. Nat. Rev. Immunol….

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Orgenesis Led Consortium awarded EUR 4M European Innovation Council Pathfinder Grant

Focus is to develop cost-effective production of autologous induced pluripotent stem cells (iPSCs) using microfluidic technologies and artificial intelligence (AI) Germantown, Maryland, USA, May 3, 2022 – Orgenesis Inc. (NASDAQ: ORGS) (“Orgenesis” or the “Company”), a global biotech company working to unlock the full potential of cell and gene therapies, today announces a consortium…

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EdiGene to Present getools, a Data Analysis Software Toolkit for Advanced Analytics of DNA and RNA Editing Efficiency and Outcomes for Therapeutic Development at the ASGCT 25th Annual Meeting

BEIJING & CAMBRIDGE, Mass.–(BUSINESS WIRE)–BEIJING and CAMBRIDGE, MASS. – (May 2, 2022) – EdiGene, Inc., a global, clinical-stage biotechnology company focused on translating gene-editing technologies into transformative therapies for patients with serious genetic diseases and cancer, will present getools (gene editing data analysis toolkit) for advanced analytics of DNA and…

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Bioinformatics Specialist at The University of Chicago in Chicago, IL – 4091

Job Info Department BSD PED – Section Administrator: Hematology, Oncology, and Stem Cell Transplantation About the Department The Section of Pediatric Hematology/Oncology provides care for patients and families with acute and chronic medical conditions, or other hematologic diseases, requiring the specialty knowledge and skills of Hematology, Oncology and Stem Cell…

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Molecular analysis of TCGA breast cancer histologic types

Breast cancer is classified into multiple distinct histologic types, and many of the rarer types have limited characterization. Here, we extend The Cancer Genome Atlas Breast Cancer (TCGA-BRCA) dataset with additional histologic type annotations, in a total of 1063 breast cancers. We analyze this extended dataset to define transcriptomic and…

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Download shrna images for free

Home Download frontiers sgrna shrna structure mediated site Download intratumoral injection shrna expressing plasmid Download intratumoral injection shrna expressing plasmid Download full text lentivirus mediated shrna targeting mutyh inhibits malignant phenotype Download silencing regiv shrna causes loss stemness properties cancer stem cells mkn45 Download upar shrna inhibit angiogenesis enhanced Download…

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Human stem cell modelling for heart disease of long QT syndrome

Abstract Sudden cardiac death (SCD) in otherwise healthy young people is a public health priority. Long QT syndrome (LQTS) is the commonest life-threatening cardiac arrhythmia contributing to SCD under 40 years old with an estimated prevalence of 1:2,000. Cardiac rhythm is driven by coordinated cardiac action potential, which is triggered…

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Postdoc Fellow on Cancer Genomics, Epigenetics, Metabolism, and Bioinformatics

Multiple postdoctoral positions are available in the lab of Dr. Jian Xu at Children’s Research Institute (CRI), UT Southwestern Medical Center to study the gene regulatory processes that control stem cell development and cancers. We employ genomics, epigenetics, genome editing, metabolomics, and mouse genetics to define disease-associated epigenetic and genetic programs…

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Rat Model Market revenue worth USD 2.3 Billion by 2028,

Selbyville, Delaware, April 26, 2022 (GLOBE NEWSWIRE) — The rat model market value is projected to reach USD 2.3 billion by 2028, according to a new research report by Global Market Insights Inc. Growing consumption of personalized medicine and increasing number of clinical trials is expected to augment the market…

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R For SEO Part 3: Data Visualisation With GGPlot2 & Wordcloud

[This article was first published on R | Ben Johnston, and kindly contributed to R-bloggers]. (You can report issue about the content on this page here) Want to share your content on R-bloggers? click here if you have a blog, or here if you don’t. R For SEO Part 3:…

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Researchers boost rice yield by overcoming tr

image: The mechanism of boosting rice yield by overcoming a trait trade-off beteen tiller number and panicle size view more  Credit: IGDB Crop breeding is largely constrained by tradeoffs among different agronomic traits. Since many of these tradeoffs are caused by gene pleiotropy, reducing gene pleiotropy may make it easier to…

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Endometriosis-related functional modules and hub genes

Introduction Endometriosis (EMS) is a chronic gynecological disease defined as implantation and periodic growth of the endometrial glands and stroma outside the uterine cavity, causing chronic pelvic pain, severe dysmenorrhea, and infertility in 10% reproductive-age women, among which the infertility rate is approximately 30–50%.1,2 Surgical excision is commonly used for…

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Direct and Indirect Culture Methods for Studying Biodegradable Implant Materials In Vitro

We introduce three methods of direct culture, direct exposure culture, and exposure culture for evaluating the in vitro cytocompatibility of biodegradable implant materials. These in vitro methods mimic different in vivo cell-implant interactions and can be applied to study various biodegradable materials. Direct and indirect culture methods for studying biodegradable…

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TF differentiation of hiPSCs at Wellcome Sanger Institute

Salary per annum: c.£33,900 Contract Length: 2 years Here at the world-famous and internationally respected Wellcome Sanger Institute, we have an exciting opportunity for a postdoctoral researcher to perform high throughput screening for transcription factor-driven differentiation in human pluripotent stem cells (hiPSCs) in order to create better in vitro differentiated cells that more faithfully…

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Crispr/Cas13a Induced Exponential Amplification for Highly Sensitive and Specific Detection of Circular Rna by Pengfei Song, Pengbo Zhang, Ke Qin, Fengxia Su, Kejian Gao, Xin Liu, Zhengping Li :: SSRN

19 Pages Posted: 23 Mar 2022 See all articles by Pengfei Song University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology Beijing University of Science and Technology…

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New neurodevelopmental disorder discovered

An Australian-led international research collaboration has discovered a new neurodevelopmental disorder after uncovering its link to a tumour suppressor gene, ending the diagnostic journey for 32 families around the world. Murdoch Children’s Research Institute (MCRI) researchers, along with their international peers, found variations in the FBXW7 gene were associated with…

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TREEFROG THERAPEUTICS LAUNCHES A $100,000 RESEARCH GRANT IN REGENERATIVE MEDICINE

Bordeaux, France / April 19th, 2022 – TreeFrog Therapeutics, a biotechnology company aimed at making safer, more efficient and more affordable cell therapies based on induced pluripotent stem cells (iPSCs), today announced the launch of The Stem Cell SpaceShot Grant, a $100,000 research funding in the field of stem cell…

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Still doubts over embryo selection based on PGT for polygenic conditions

Advances in genomic technologies continue to expand the possibilities of PGT. In a preclinical research study recently published in Nature Medicine, scientists from Silicon Valley-based genetic testing companies have examined the potential of using PGT to predict complex polygenically inherited conditions in human embryos. While the authors present interesting data,…

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boxplot – Ggplot geom_boxplot showing incorrect upper whisker and additional outlier

I am not sure why my boxplot created with ggplot geom_boxplot is showing an incorrect upper whisker and showing the data point (value = 7) as an outlier for “Male” grouping red boxplot. I have analyzed the same data sheet in SPSS and confirmed the Q1, Q3, and other values…

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Plenti Cmv Gfp Recipes

Transplantation of mesenchymal stem cells overexpressing Recipes Details: (Fitchburg, WI). The control plasmid, pLenti–CMV–GFP-2A-Puro and pLenti–CMV–GFP-2A-Puro vectors were purchased from Applied Biological Materials Inc (Richmond, BC, Canada). The in-formation for different antibodies used for this study is provided in Table 1. 2.2 Animals Thirty-nine male, Sprague-Dawley (SD) rats (Charles River,…

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Diffuse Large B-Cell Lymphoma: Causes, Symptoms, Treatment

Diffuse large B-cell lymphoma (DLBCL) is a cancer of the B cells of the lymphatic system. B cells are responsible for producing antibodies, which are highly specific proteins that fight against bacterial and viral infections. When cancer develops in the B cells, it is called a B-cell lymphoma. B-cell lymphoma…

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CRISPRi for specific inhibition of miRNA clusters and miRNAs with high sequence homology

Bartel, D. P. MicroRNAs: Target recognition and regulatory functions. Cell 136, 215–233 (2009). CAS  Article  Google Scholar  Bassett, A. R. et al. Understanding functional miRNA–target interactions in vivo by site-specific genome engineering. Nat. Commun. 5, 4640 (2014). ADS  CAS  Article  Google Scholar  Moore, M. J. et al. miRNA–target chimeras reveal…

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N6-methyladenosine modification of CENPK mRNA by ZC3H13 promotes cervical cancer stemness and chemoresistance | Military Medical Research

Bioinformatics analyses revealed the involvement of m6A modification in cervical cancer progression To better understand whether and how m6A regulators contribute to cervical cancer progression, we first identified 9 m6A writers (WTAP, ZC3H13, METTL3, METTL14, METTL16, VIRMA, RBM15B, RBM15, and CBLL1), 15 m6A readers (FMR1, hnRNPA2B1, hnRNPC, YTHDF1/2/3, YTHDC1/2, LRPPRC,…

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Simplified, High-throughput Analysis of Single-cell Contractility using Micropatterned Elastomers

Cell generated mechanical forces are essential to proper function in various organs throughout the body. Plus the intestines bladder, heart, and others. These organs must generate stable patterns of cell contraction and relaxation, to maintain the internal hemostatic state. Abnormal smooth muscle cell contraction can lead to the onset of…

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Nature Papers on CRISPR-Cas9 Gene Editing, HSC Development

New details about the mechanism by which DNA is unwound so that its target sequence can be read during CRISPR-Cas9 gene editing are reported in this week’s Nature Structural & Molecular Biology. The study provides insights that may help in the development of improved genome editors. During CRISPR-Cas9 editing, Cas9…

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Glioblastoma scRNAseq Shows Treatment-induced, Immune-dependent Rise In Mesenchymal Cancer Cells, and Structural Variants in Distal Neural Stem Cells

This article was originally published here Neuro Oncol. 2022 Apr 13:noac085. doi: 10.1093/neuonc/noac085. Online ahead of print. ABSTRACT BACKGROUND: Glioblastoma is a treatment-resistant brain cancer. Its hierarchical cellular nature and its tumour microenvironment (TME) before, during, and after treatments remain unresolved. METHODS: Here, we used single-cell RNA-sequencing to analyze new…

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Building better models of brain aging and Alzheimer’s disease

Every moment counts in the pursuit of effective preventions and treatments for age-associated diseases affecting the brain. Capturing the essence of how aging and dementias such as Alzheimer’s disease affect the brain without the need to wait for aging to take place in real time has been a long-standing obstacle…

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Postdoctoral Researcher in Genomics and Bioinformatics – Karolinska Institute – job portal

Login and apply Do you want to contribute to top quality medical research? The Department of Cell and Molecular Biology (CMB) is a department with a strong focus on basic science. CMB conducts research and education in cell biology, molecular biology, developmental biology, stem cell biology, cancer and infection biology….

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Transcriptional and H3K27ac related genome profiles in oral squamous cell carcinoma cells treated with metformin

. 2022 Mar 21;13(6):1859-1870. doi: 10.7150/jca.63234. eCollection 2022. Affiliations Expand Affiliations 1 State Key Laboratory of Oral Diseases & National Clinical Research Center for Oral Diseases & Department of Head and Neck Oncology, West China Hospital of Stomatology, Sichuan University, Chengdu, China. 2 Department of Oral and Maxillofacial Surgery, Binzhou…

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Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules

Li, Zhang, Yin, Yang, Du, Hou, Ge, Liu, Zhang, Zhang, Wu, Li, Liu, Wu, Song, Zhao, Shi, Deng (2011) Generation of iPSCs from mouse fibroblasts with a single gene, Oct4, and small molecules Cell Res 21(1) 196-204 Full text Abstract The introduction of four transcription factors Oct4,…

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Optimization of conditions for intrauterine electroporation of neural stem cells in the subependymal region of fetal rat brains

Explanation of the title: Neural precursor cells: Multipotent cells capable of self-proliferation and differentiation, which can maintain the desired number of neural precursor cells through proliferation, and can also differentiate into neurons, astrocytes and oligodendrocytes. In utero electrotransfection technique: In utero electroporation is an important technique to study the molecular…

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Researchers Discover New Neurodevelopmental Disorder

Researchers have discovered a new neurodevelopmental disorder after uncovering its link to a tumor suppressor gene. Credit: Edmonton Economic Development Corporation Australian scientists were recently able to give long-sought after answers to 35 people and families when they discovered a new neurodevelopmental disorder after uncovering its link to a tumor…

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Parkinson’s disease motor symptoms rescue by CRISPRa-reprogramming astrocytes into GABAergic neurons

doi: 10.15252/emmm.202114797. Online ahead of print. Jessica Giehrl-Schwab #  1   2 , Florian Giesert #  1   2 , Benedict Rauser  1   2 , Chu Lan Lao  3   4 , Sina Hembach  1   2 , Sandrine Lefort  5 , Ignacio L Ibarra  6 , Christina Koupourtidou  3   7 , Malte Daniel Luecken  6 , Dong-Jiunn Jeffery…

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Single-Cell Technology Measures Epigenetic States and Gene Expression in One Go

Advances in single-cell technologies have not only made it possible to analyze multiple molecular families in individual cells, they have also enabled scientists to parse through limitations in data analysis arising from the intercellular diversity of measured parameters. Although single-cell sequencing is now widely available, techniques to detect epigenetic markers…

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Postdoc – Human Genome Engineering, Cell Therapies, iPSCs, Synthetic Biology

The Truong lab is hiring for multiple Postdoctoral Fellow positions at NYU. We use large scale human genome engineering to develop off-the-shelf cell therapies using iPSCs.   The lab uses Synthetic Biology to build allogeneic Dendritic Cells from iPSCs as a platform for cell immunotherapies. We are interested in all…

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Researchers identify sustainable source of im

image: Monkey iPSCs with edited CCR5 locus growing in culture. view more  Credit: Image Credit: Saritha D’Souza, Wisconsin National Primate Research Center, USA Genetic engineering can make immune cells resistant to infection with human or monkey (simian) immunodeficiency virus (HIV and SIV, respectively). Recently, treatment with HIV-resistant immune cells has given…

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Human distal lung maps and lineage hierarchies reveal a bipotent progenitor

Verleden, S. E. et al. Small airways pathology in idiopathic pulmonary fibrosis: a retrospective cohort study. Lancet Respir. Med. 8, 573–584 (2020). CAS  PubMed  PubMed Central  Google Scholar  Hogg, J. C., Macklem, P. T. & Thurlbeck, W. M. The resistance of small airways in normal and diseased human lungs. Aspen…

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Absolute Scaling of Single-Cell Transcriptomes Reveals Pervasive Hypertranscription in Adult Stem and Progenitor Cells

Introduction Single cell RNA-seq (scRNA-seq) is a powerful tool to measure gene expression in individual cells. While relative gene expression differences are currently analyzed with great interest in single cell data, differences in total transcript levels were mainly considered technical artefacts and removed during normalization, assuming that single cells contain…

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Postdoctoral Position in Bioinformatics to study Gene Regulatory Networks

Understanding how a fertilized egg develops into a complex multicellular organism is one of the most fascinating topics in biology. The current challenge is to understand how genes function as part of gene regulatory networks (GRNs) that control the proliferation and patterning of progenitor and stem cells during embryonic development….

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2D and 3D Human Induced Pluripotent Stem Cell-Based Models to Dissect Primary Cilium Involvement during Neocortical Development

We present detailed protocols for the generation and characterization of 2D and 3D human induced pluripotent stem cell (hIPSC)-based models of neocortical development as well as complementary methodologies enabling qualitative and quantitative analysis of primary cilium (PC) biogenesis and function. The overall goal of the procedure we detail here is…

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Modeling the Human Brain with Organoids

iPSCs As Models, Part 2: Modeling the Human Brain with Organoids The store will not work correctly in the case when cookies are disabled. JavaScript seems to be disabled in your browser. For the best experience on our site, be sure to turn on Javascript…

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Polatuzumab Vedotin Plus R-CHP Recommended for EU Approval for Previously Untreated DLBCL

The European Medicines Agency’s Committee for Medicinal Products for Human Use has adopted a positive opinion in favor of the approval of polatuzumab vedotin for use in combination with rituximab plus cyclophosphamide, doxorubicin, and prednisone in patients with previously untreated diffuse large B-cell lymphoma. The European Medicines Agency’s Committee for…

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Identification of potentially functional circular RNAs hsa_circ_0070934 and hsa_circ_0004315 as prognostic factors of hepatocellular carcinoma by integrated bioinformatics analysis

Rawla, P., Sunkara, T., Muralidharan, P. & Raj, J. P. Update in global trends and aetiology of hepatocellular carcinoma. Contemp. Oncol. (Poznan, Poland) 22, 141–150 (2018). CAS  Google Scholar  Kong, D. et al. Current statuses of molecular targeted and immune checkpoint therapies in hepatocellular carcinoma. Am. J. Cancer Res. 10,…

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Gopal Addresses Differences Between Real-World and Clinical Trial Data for DLBCL

During a Targeted Oncology case-based roundtable event, Ajay K. Gopal, MD, discussed the options for second-line and subsequent treatment of a patient with relapsed/refractory diffuse large B-cell lymphoma who refuses CAR T-cell therapy. Targeted OncologyTM: What are the recommended approaches for relapsed/refractory DLBCL in the second-line and subsequent therapies settings?…

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Nanofountain Probe Electroporation system enables efficient engineering of stem cells — ScienceDaily

One of the ultimate goals of medical science is to develop personalized disease diagnostics and therapeutics. With a patient’s genetic information, doctors could tailor treatments to individuals, leading to safer and more effective care. Recent work from a team of Northwestern Engineering researchers has moved the field closer to realizing…

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Using CRISPR/Cas to develop biosafety materials

The diagnosis and treatment of viral infections, especially those that cause large-scale outbreaks, is urgently needed, as the lack of these measures allows epidemics to progress. A new Biosafety and Health study discusses the use of the highly efficient clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated proteins (Cas) technology to…

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Regenerative therapies with induced pluripotent stem cells

Induced pluripotent stem cells (iPSCs) are a type of stem cell with pluripotent characteristics. That is, they have the capacity to generate most of the tissues and cells of all adult cell types. They are obtained artificially from a cell that was initially not pluripotent. Due…

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Sigma-Aldrich Files Opposition to CVC Substantive Preliminary Motion No. 1 to be Accorded Priority Benefit | McDonnell Boehnen Hulbert & Berghoff LLP

On February 18th, Sigma-Aldrich filed its Opposition to Junior Party’s (the University of California, Berkeley; the University of Vienna; and Emmanuelle Charpentier; collectively, “CVC”) Substantive Preliminary Motion No. 1 in Interference No. 106,132, asking the Patent Trial and Appeal Board for benefit of priority to U.S. Provisional Application No. 61/652,086,…

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Time for Proteomics To Shine

This article includes research findings that are yet to be peer-reviewed. Results are therefore regarded as preliminary and should be interpreted as such. Find out about the role of the peer review process in research here. For further information, please contact the cited source. Over the last 20 years, advances…

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A single-cell atlas of human and mouse white adipose tissue

Rosen, E. D. & Spiegelman, B. M. What we talk about when we talk about fat. Cell 156, 20–44 (2014). CAS  PubMed  PubMed Central  Google Scholar  Kahn, S. E., Hull, R. L. & Utzschneider, K. M. Mechanisms linking obesity to insulin resistance and type 2 diabetes. Nature 444, 840–846 (2006)….

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Programming skills in biocuration | EMBL-EBI Training

This webinar will be the first of our series of webinars on the topic of biocuration and discuss programming skills that can be beneficial in biocuration, as well as provide advice on how to acquire them. In an ELIXIR survey in 2019 which aimed at identifying training needs…

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Function of SYDE C2-RhoGAP family as signaling hubs for neuronal development deduced by computational analysis

Hallam, S. J., Goncharov, A., McEwen, J., Baran, R. & Jin, Y. Syd-1, a presynaptic protein with PDZ, C2 and rhoGAP-like domains specifies axon identity in C. elegans. Nat. Neurosci. 5, 1137–1146 (2002). CAS  PubMed  Google Scholar  Xu, Y. & Quinn, C. C. SYD-1 promotes multiple developmental steps leading to…

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Enhanced cGAS-STING-dependent interferon signaling associated with mutations in ATAD3A

dc.identifier.citation Lepelley , A , Della Mina , E , Van Nieuwenhove , E , Waumans , L , Fraitag , S , Rice , G I , Dhir , A , Fremond , M-L , Rodero , M P , Seabra , L , Carter , E , Bodemer…

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Human induced pluripotent stem cells improve visual acuity, vascular health — ScienceDaily

Researchers at Indiana University School of Medicine, in collaboration with the University of Alabama at Birmingham and five other institutions, are investigating novel regenerative medicine approaches to better manage vascular health complications from type 2 diabetes that could someday support blood vessel repair in the eye among diabetic patients with…

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Anaplastic Large Cell Lymphoma Presenting as Ulcerative Facial Mass: A Case Report – FullText – Case Reports in Oncology 2022, Vol. 15, No. 1

Anaplastic large cell lymphoma (ALCL) is a rare form of non-Hodgkin lymphoma (NHL) that can be aggressive with rapid speed, thus mandating a timely diagnosis to optimize treatment and deter progression. NHL classically presents with lymphadenopathy and constitutional symptoms. However, ALCL can present with nonspecific cutaneous manifestations with minimal or…

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Prediction of histone post-translational modification patterns based on nascent transcription data

Allfrey, V. G., Faulkner, R. & Mirsky, A. E. Acetylation and methylation of histones and their possible role in the regulation of RNA synthesis. Proc. Natl Acad. Sci. USA 51, 786–794 (1964). CAS  PubMed  PubMed Central  Google Scholar  Ho, J. W. K. et al. Comparative analysis of metazoan chromatin organization….

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A hydrogel-based 3D culture model for Down syndrome-myeloid leukemia and ex vivo drug testing

Abstract Induced pluripotent stem cells (iPSCs) provide an extraordinary tool for disease modeling owing to their potential to differentiate into the desired cell type. The differentiation of iPSCs is typically performed on 2-dimensional monolayers of stromal cell or animal tissue derived extracellular matrices. Recent advancements in disease modeling have utilized…

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Study of Dendritic Cell Development by Short Hairpin RNA-Mediated Gene Knockdown in a Hematopoietic Stem and Progenitor Cell Line In vitro

Here we provide a protocol for screening potential transcription factors involved in the development of dendritic cell (DC) using lentiviral transduction of shRNA to obtain stable knockdown cell lines for in vitro DC differentiation. Dendritic cells, or DCs, are key immune cells linking the innate and adaptive immune system. While…

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Endothelial Cell Differentiation from iPSCs – iPSC Differentiation Service – Stem Cell Services – Service – Research

iPSC-Derived Endothelial Cells Optimized iPSC Generation & Differentiation Protocols High Purity & Well Characterized iPSC-derived endothelial cells (EC) are valuable tools that can help researchers better understand vascular diseases. At ASC, we use a fast, cost-effective differentiation procedure that allows our team to deliver high-quality cells in…

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Precision Control of Culture Conditions for Induced Pluripotent Stem Cells

By Sally Temple The Nobel Prize–winning discovery that mature, specialized cells can be reprogrammed to lend them pluripotency (that is, the ability to develop into all tissues of the body) unleashed vast potential in the fields of disease modeling, drug screening, regenerative medicine, and wound healing. The reprogrammed cells are…

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DNMT1 is a negative regulator of osteogenesis

This article was originally published here Biol Open. 2022 Mar 15;11(3):bio058534. doi: 10.1242/bio.058534. Epub 2022 Mar 3. ABSTRACT The role and underlying mechanisms of DNA methylation in osteogenesis/chondrogenesis remain poorly understood. We here reveal DNA methyltransferase 1 (DNMT1), which is responsible for copying DNA methylation onto the newly synthesized DNA…

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Research Associate job with KINGS COLLEGE LONDON

Job description A Postdoctoral Research Associate (PDRA) – Computational biology position is available in the Centre for Gene Therapy and Regenerative Medicine, King’s College London. This is an exciting opportunity to join an interdisciplinary team of scientists working on a Wellcome Trust funded research programme aimed at studying the mammalian…

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Modeling injury and repair in kidney organoids reveals that homologous recombination governs tubular intrinsic repair

Submission history Received: 28 May 2021 Accepted: 14 January 2022 Acknowledgments We thank J. V. Bonventre, J. A. Lewis, and E. P. Rhee for institutional support; S. J. Hill for insightful discussion and sharing antibodies; Y. Yoda for cell culture; L. Racusen of Johns Hopkins University for the HKC-8 cell…

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Frontiers | Hair Graying Regulators Beyond Hair Follicle

Introduction Hair graying is one of the representative signs of aging. It has been considered to be triggered by a decreased number of follicular melanocyte stem cells (MeSCs; Nishimura et al., 2005; Iida et al., 2020) or dysfunction of MeSCs such as decreased oxidation resistance capacity with aging (Shi et…

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GPP Web Portal – Transcript Details

Transcript: Human XR_933717.2 PREDICTED: Homo sapiens uncharacterized LOC105371334 (LOC105371334), ncRNA. Source: NCBI, updated 2019-09-08 Taxon: Homo sapiens (human) Gene: LOC105371334 (105371334) Length: 321 CDS: (non-coding) sgRNA constructs matching this transcript (CRISPRko, NGG PAM) This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the exonic sequence of…

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Exosomal microRNA-618 derived from mesenchymal stem cells attenuate the progression of hepatic fibrosis by targeting Smad4

Introduction Hepatic fibrosis (HF) is a pathological phenomenon produced in the process of long-term damage and repair in the liver [1,2]. During the formation of HF, hepatocytes are damaged by various factors, such as chronic liver disease, and excessive intake of alcohol, and secrete a variety of cytokines [3]. Subsequently,…

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Connecting science to medicine: tendon-like t

image: (a) Comparison of bio-tendons generated from GFP-iPSC-MSCs and Mkx-iPSC-MSCs. (b) SEM images of the surface layer of GFP-bio-tendon and Mkx-bio-tendon. view more  Credit: Department of Systems BioMedicine, TMDU Researchers from Tokyo Medical and Dental (TMDU) develop artificial tendons in vitro from human stem cells that could fix common tendon injuries…

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Notch activation suppresses endothelial cell migration and sprouting via miR-223-3p targeting Fbxw7

Alabi RO, Farber G, Blobel CP (2018) Intriguing roles for endothelial ADAM10/Notch signaling in the development of organ-specific vascular beds. Physiol Rev 98:2025–2061 CAS  Article  Google Scholar  Autiero M, De Smet F, Claes F, Carmeliet P (2005) Role of neural guidance signals in blood vessel navigation. Cardiovasc Res 65:629–638 CAS …

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Generation and characterization of motor neuron progenitors and motor neurons using metachromatic leukodystrophy-induced pluripotent stem cells

doi.org/10.1016/j.ymgmr.2022.100852Get rights and content Highlights • We generated induced pluripotent stem cells (iPSCs) from two late-infantile MLD patient-derived skin fibroblasts. • We differentiated MLD phenotype specific motor neurons (MNs) under the culture condition. • We described the pathological observation of MLD patients derived motor neuron progenitors (MNPs). • We described…

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Generation of Hook Ischemia-Reperfusion Model using a Three-Day Developing Chick Embryo

This paper describes ischemia-reperfusion (I/R) modeling in a 3-day chick embryo using a spinal needle customized hook to better understand I/R development and treatment. This model is simple, quick, and inexpensive. Hi, I am Dr.Syed Shadab Raza, the principal investigator for the Laboratory for Stem Cells and Restorative Neurology, located…

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