Tag: SV40

Did COVID vaccines kill 14X more people than they saved?

Did a study just show that COVID-19 vaccines killed 14x more people than they saved?! Take a look at the viral claim, and find out what the facts really are!   Claim : COVID Vaccines Kill 14X More People Than They Saved! People are sharing an article (archive) by The…

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Amid second-largest wave of COVID infections in the US, Florida’s surgeon general calls to halt mRNA vaccines

On Friday, wastewater data from Biobot Analytics and the US Centers for Disease Control and Prevention (CDC) affirmed that the US has now reached its second-highest level of COVID-19 infections of the pandemic. It is anticipated that by midweek, rates of infections will reach 2 million per day with approximately…

Continue Reading Amid second-largest wave of COVID infections in the US, Florida’s surgeon general calls to halt mRNA vaccines

Frontiers Publishing Partnerships | Construction of recombinant adenovirus-5 vector to prevent replication-competent adenovirus occurrence

Introduction In recent years, recombinant adenoviral vectors have been used in different fields of biomedical sciences such as in vitro and in vivo gene transfer, vaccine development, and gene therapy (Russell, 2000; Mitani and Kubo, 2002). Multiple features of recombinant adenoviral vectors such as high packaging capacity for transgene insertion,…

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FDA Fails to Address DNA Adulteration Concerns

The following article originally appeared on Dr. Robert Malone’s Substack, and is reprinted here verbatim with permission. Robert Malone, MD, MS; Malone Institute December 15, 2023, 2:30 PM EST (Madison VA and Tallahassee FL) The failure of government regulatory authorities to identify and disclose DNA fragment contamination of…

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FDA responds to Ladapo letter

In response to a letter in which Florida Surgeon General Joseph Ladapo noted the detection of DNA fragments in mRNA COVID-19 vaccines, Peter Marks, director of the U.S. Food and Drug Administration’s Center for Biologics Evaluation and Research, emphasized the FDA’s view that the shots are safe and effective. “I…

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Exclusive interview: Florida Surgeon General Joseph Ladapo asks FDA for data on risks of DNA contaminants in COVID-19 vaccines

Florida Surgeon General Joseph Ladapo | Photo courtesy of FDOH BY JENNIFER CABRERA TALLAHASSEE, Fla. – On December 6, Florida Surgeon General Joseph Ladapo sent a letter to the U.S. Food and Drug Administration (FDA), asking questions about a recent preprint documenting the detection of billions of DNA fragments per…

Continue Reading Exclusive interview: Florida Surgeon General Joseph Ladapo asks FDA for data on risks of DNA contaminants in COVID-19 vaccines

Human RAD52 stimulates the RAD51-mediated homology search

Introduction Homologous recombination (HR) is an evolutionarily conserved process that plays a pivotal role in genome stability, diversity, and plasticity. HR is indeed a key repair pathway able to faithfully repair DNA damages including double-strand breaks (DSBs) and DNA gaps by copying the error-free information from the template DNA normally…

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Fla. Surgeon General Seeks Answers on DNA Contamination of Covid Shots

bymuratdeniz/iStock/Getty Images Plus Florida’s Surgeon General, Dr. Joseph Ladapo, is seeking an explanation from federal health authorities on the undisclosed presence of potentially dangerous DNA fragments in mRNA Covid-19 injections. Ladapo sent a letter to U.S. Food and Drug Administration (FDA) Commissioner Dr. Robert Califf and the U.S. Centers…

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Ladapo letter DNA fragments COVID-19 vaccines

Florida Surgeon General Joseph Ladapo, who notes that DNA fragments have been detected in mRNA COVID-19 vaccines, has issued a letter asking questions related to the issue. “On today’s episode of: What the FDA… I asked @DrCaliff_FDA to address the DNA fragments detected in mRNA COVID shots & how they…

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DNA Sequence in Pfizer COVID-19 Vaccine Could Spur New Lawsuits: Lawyers

A woman walks by Pfizer’s headquarters in New York City in a file image. (Kena Betancur/AFP via Getty Images) Pfizer and its partner BioNTech could be open to lawsuits for including a DNA sequence in their COVID-19 vaccine. The Public Readiness and Emergency Preparedness Act, known as the PREP Act,…

Continue Reading DNA Sequence in Pfizer COVID-19 Vaccine Could Spur New Lawsuits: Lawyers

Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease

mitoARCUS localizes to the mitochondrial matrix To evaluate the ability of mitoARCUS to specifically cleave m.3243G mutant mtDNA, cell lines containing varying levels of the mutation were generated. All of these cell lines were isolated from the same parental m.3243A>G cybrid cell line. Levels of the m.3243G mutation of each…

Continue Reading Efficient elimination of MELAS-associated m.3243G mutant mitochondrial DNA by an engineered mitoARCUS nuclease

DNA Contamination In COVID-19 Vaccines May Explain Rise In Cancers, Clots, Autoimmune Diseases: Pathologist

Authored by Tom Ozimek and Jan Jekielek via The Epoch Times (emphasis ours), Clinical pathologist Dr. Ryan Cole has said that DNA contamination in some COVID-19 vaccines may be related to an increase in cancers, micro-clotting, and autoimmune diseases. “My big concern is the fact that billions of people across…

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NOT REAL NEWS: A look at what didn’t happen this week

A roundup of some of the most popular but completely untrue stories and visuals of the week. None of these are legit, even though they were shared widely on social media. The Associated Press checked them out. Here are the facts: ___ No evidence that DNA sequence used in Pfizer…

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A look at what didn’t happen this week

FILE – A health worker administers a dose of COVID-19 vaccine during a vaccination clinic in Reading, Pa. On Friday, Nov. 24, 2023, The Associated Press reported on stories circulating online incorrectly claiming Pfizer’s COVID-19 vaccine contains a DNA sequence called Simian Virus 40 that can cause health problems, including…

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No evidence that DNA sequence used in Pfizer shot leads to cancer and other health issues

CLAIM: Pfizer’s COVID-19 vaccine contains a DNA sequence called Simian Virus 40 that can cause health problems, including cancer. AP’S ASSESSMENT: False. No evidence has been found to suggest DNA fragments used in the development of the coronavirus vaccine — such as a portion of SV40’s DNA sequence — are…

Continue Reading No evidence that DNA sequence used in Pfizer shot leads to cancer and other health issues

Moderna Admits DNA Contamination Can Lead To Cancer

It’s been revealed that Moderna said that the DNA contamination in the vaccines can lead to cancer. Check out the latest reports about the matter below. Here is the tweet that sheds more light on the matter, along with the video featuring dr. Malone. “Moderna ADMITS That DNA Contamination Can…

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Moderna Admits MRNA Jabs Cause Turbo-Cancer; Investigators Find Billions Of DNA Fragments In Vials

Moderna has admitted that its experimental Covid-19 vaccines contain billions of DNA fragments, which experts have warned cause turbo-cancer in humans. Dr. Robert Malone made an appearance this week at an “Injuries Caused by COVID-19 Vaccines” hearing led by Congresswoman Marjorie Taylor Greene, where he revealed how Moderna’s own patent proves that its mRNA…

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There are no genetically modified organisms in the COVID-19 mRNA vaccines; no evidence these vaccines modify our DNA

CLAIM Unapproved genetically modified organisms in the COVID-19 mRNA vaccines could permanently change our DNA DETAILS Factually inaccurate: There are no genetically modified organisms in the COVID-19 mRNA vaccines.Inadequate support: The studies cited to support the claim that vaccine mRNA can modify the human genome are unrelated to vaccination or…

Continue Reading There are no genetically modified organisms in the COVID-19 mRNA vaccines; no evidence these vaccines modify our DNA

Plasmid DNA Contamination In COVID-19 Vaccines Is ‘Clear Breach Of Informed Consent’ By FDA: Dr. Malone

Authored by Naveen Athrappully via The Epoch Times (emphasis ours), Dr. Robert Malone, the inventor of mRNA and DNA vaccines, slammed health regulatory agencies for not informing people about potential plasmid DNA contamination in mRNA COVID-19 vaccines. A health care worker prepares a dose of Pfizer BioNTech COVID-19 vaccine in…

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Moderna Admitted That DNA Contamination Can Lead To Cancer

It has been revealed that Moderna admitted that DNA contamination can lead to cancer. Check out the following tweet, which shows an important video as well. DNA contamination vaccines Here is the post on X that sheds light on the matter: “Moderna ADMITS That DNA Contamination Can Lead to Cancer…

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Billions Of Copies Of Residual DNA In A Single Dose Of COVID-19 mRNA Vaccine: Preprint

A new preprint study up for peer review finds billions of residual DNA fragments in the COVID-19 mRNA vaccine vials. The lead author of the study, molecular virologist David Speicher, who has a doctorate in virology, told The Epoch Times that their study is “the largest study” on residual DNA…

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Are Residual DNA In mRNA Vaccines Dangerous?!

Are residual DNA impurities in mRNA vaccines dangerous, and can they integrate into our DNA?! Take a look at the viral claims, and find out what the facts really are!   Claim : Residual DNA In mRNA Vaccines Are Dangerous! Anti-vaccine activists are sharing and/or promoting an article by The…

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FDA Responds After Being Urged To Recall Pfizer’s Vaccine Over DNA Fragments

Authored by Zachary Stieber via The Epoch Times (emphasis ours), The U.S. Food and Drug Administration (FDA) is refusing to recall the Pfizer-BioNTech COVID-19 vaccine, promoting the view that the inclusion of a previously-undisclosed DNA sequence that leaves behind fragments is not of concern. A vial of Pfizer’s COVID-19 vaccine…

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Posts Falsely Claim FDA ‘Required’ to Take mRNA COVID-19 Vaccines Off Market Due to Adulteration

SciCheck Digest As with many medical products, the mRNA COVID-19 vaccines may contain trace amounts of residual DNA from the manufacturing process. It is not considered adulteration, and the Food and Drug Administration is not “required” to pull the vaccines from the market, contrary to viral claims online. How do…

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European Regulator Confirms Pfizer Did Not Highlight DNA Sequence In COVID-19 Vaccine

Authored by Zachary Stieber via The Epoch Times (emphasis ours), Pfizer did not highlight a DNA sequence in its COVID-19 vaccine, a European regulator has confirmed. “While the full DNA sequence of the plasmid starting material was provided in the initial marketing authorization application for Comirnaty, the applicant did not…

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Residual DNA in COVID-19 mRNA vaccines is documented, isn’t associated with genome modification or cancer, contrary to claim by Robert Malone

CLAIM The more COVID-19 vaccines doses people receive, the higher the risk of getting COVID-19; vaccines contain DNA fragments that modify the genome and cause cancer DETAILS Inadequate support: The claim that vaccinated people are more likely to get COVID-19 or aggressive cancers isn’t supported by evidence. In fact, epidemiological…

Continue Reading Residual DNA in COVID-19 mRNA vaccines is documented, isn’t associated with genome modification or cancer, contrary to claim by Robert Malone

Did EMA Confirm Pfizer Hid SV40 DNA In Vaccine?!

Did the EMA (European Medicines Agency) just confirm that Pfizer hid the presence of SV40 DNA in its mRNA COVID-19 vaccine? Take a look at the viral claim, and find out what the facts really are!   Claim : EMA Confirmed Pfizer Hid SV40 DNA In Vaccine! Some people are…

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Posts misrepresent Health Canada statement on DNA in Covid vaccines

Screenshot of an Instagram post taken October 30, 2023 Similar claims ricocheted across platforms such as TikTok, Instagram, Facebook and X, formerly known as Twitter. The posts build on the debunked narrative that Covid-19 vaccines contain harmful DNA particles that could alter recipients’ genetics. The Children’s Health Defense article suggests…

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Experts say there’s no evidence that the mRNA COVID-19 vaccines are ‘adulterated’

Vaccines contaminated with undisclosed ingredients. Manufacturers facing liability. In an Oct. 21 thread on X, entrepreneur and anti-vaccine activist Steve Kirsch made alarming allegations about mRNA COVID-19 vaccines. “You can now sue the mRNA COVID vaccine manufacturers for damages and the FDA is required to take the COVID vaccines off…

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Are mRNA Vaccines Contaminated With SV40 DNA?!

Are mRNA COVID-19 vaccines from Pfizer and Moderna contaminated with SV40 virus DNA that can cause turbo cancer and other health problems?! Take a look at the viral claims, and find out what the facts really are!   Claim : mRNA Vaccines Are Contaminated With SV40 DNA! Some people are…

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FDA Should Recall ‘Adulterated’ Pfizer COVID-19 Vaccine: Robert Malone

Authored by Zachary Stieber via The Epoch Times (emphasis ours), Pfizer’s COVID-19 vaccine is adulterated due to the undisclosed presence of a DNA sequence, experts say. A health worker fills a syringe with a Pfizer-BioNTech COVID-19 vaccine in a file image. (Emmi Korhonen /Lehtikuva/AFP via Getty Images) That should prompt…

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Experts rebut claims that mRNA COVID-19 vaccines are ‘adulterated’

Vaccines contaminated with undisclosed ingredients. Manufacturers facing liability. In an Oct. 21 thread on X, entrepreneur and anti-vaccine activist Steve Kirsch made alarming allegations about mRNA COVID-19 vaccines.  “You can now sue the mRNA COVID vaccine manufacturers for damages and the FDA is required to take the COVID vaccines off…

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An alarming revelation: DNA plasmid contamination in Pfizer COVID vaccines

Julie Reeder In a startling revelation that has sent shockwaves through the medical and scientific community, Health Canada recently confirmed the presence of DNA contamination in Pfizer’s COVID-19 vaccines. This confirmation is not just an ordinary blip on the radar; it’s an admission of epic proportions that raises significant concerns…

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COVID-19 Vaccines Have Not Been Shown to Alter DNA, Cause Cancer

SciCheck Digest Small amounts of DNA from the manufacturing process may remain in the mRNA COVID-19 vaccines. Purification and quality control steps ensure any leftover DNA is present within regulatory limits. There isn’t reason to think that this residual DNA would alter a person’s DNA or cause cancer, contrary to claims made…

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Did Health Canada Confirm SV40 DNA In Pfizer Vaccine?!

Did Health Canada just confirm the presence of SV40 DNA in the Pfizer mRNA vaccine for COVID-19?! Take a look at the viral claim, and find out what the facts really are!   Claim : Health Canada Confirmed SV40 DNA In Pfizer Vaccine! Some people are sharing an article by…

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‘Health Canada hasn’t considered the risk’ of COVID vaccine DNA contamination issue, says citizen scientist

The shocking discovery comes in the form of a pre-print paper wherein citizen scientists have investigated the levels of residual DNA found within various vials and lots…

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Contrary to viral claim, regulatory agencies knew of residual DNA in COVID-19 mRNA vaccines; no evidence this poses health concern

CLAIM Regulatory agencies didn’t know of residual DNA contamination in COVID-19 RNA vaccines; residual DNA could integrate into our DNA and cause cancer DETAILS Factually inaccurate: Health Canada stated that it was aware of residual DNA in COVID-19 mRNA vaccines before the vaccines were authorized. A dossier submitted by BioNTech…

Continue Reading Contrary to viral claim, regulatory agencies knew of residual DNA in COVID-19 mRNA vaccines; no evidence this poses health concern

Fact Check: FDA Is NOT ‘Required To Take The COVID Vaccines Off The Market’

No Basis Is the Food and Drug Administration (FDA) “required to take the COVID vaccines off the market” as of October 21, 2023? No, that’s not true: COVID-19 vaccines are not being taken off the market by the FDA as of this writing. All vaccines that were available in October…

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What is Simian Virus 40 DNA?

What is Simian Virus 40 DNA Simian Virus 40 (SV40) DNA is a small, circular, double-stranded DNA virus that belongs to the polyomavirus family. It was first discovered in 1960 as a contaminant in polio vaccines derived from monkey kidney cells. SV40 gained significant attention due to its potential association…

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Health Canada stands firm: Pfizer vaccine safe and effective despite undisclosed contaminants

Health Canada continues its endorsement and authorization of novel COVID-19 mRNA injections for everyone aged six months and up, despite confirmation that Pfizer…

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The Age of Aquarius – RNA, the Epicenter of Genetic Information

The veil on genetic information was lifted by the advent of gene cloning and sequencing technologies in the 1970s. The discovery and purification of bacterial nucleases capable of cutting DNA at specific sequences and ligases capable of joining them enabled DNA from any species – or mRNAs converted to complementary…

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Contamination in mRNA Shots ‘Serious Regulatory Oversight’

The discovery of excessive levels of DNA contamination in the mRNA COVID shots has raised questions about how it got there, and what it means for people who may have been injected with contaminated products. Scientists have some answers, and a lot of questions… Earlier this year, Umbrella News reported…

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Health Canada Reveals Unreported DNA Sequence in Pfizer Vaccine

Canadian health officials recently discovered an unreported DNA fragment in Pfizer’s COVID-19 vaccine. Health Canada stated to Epoch Times that Pfizer didn’t disclose this particular SV40 DNA sequence during its application process. Scientific experts have diverging opinions on the importance of this revelation. When drug manufacturers submit vaccines for review,…

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Health Canada Confirms Presence Of A Monkey Virus In DNA Sequence Of Pfizer Vaccine, Report Says

It sounds like humans were a part of a mad scientist’s experiment. In an exclusive email to The Epoch Times, Health Canada has reportedly disclosed their disturbing findings about the Pfizer Covid-19 vaccine. According to the agency, there is the presence of a Simian Virus 40 (SV40) – a monkey…

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Health Canada’s startling admission confirms secret DNA contamination discovered in COVID-19 vaccines

Health Canada, responsible for authorizing and regulating the novel mRNA injections, admitted that they have confirmed the presence of at least one plasmid…

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Researchers Warn Molnupiravir Causes New Variants; mRNA Shots Contaminated with DNA Fragments

by Debra Heine   Medical scientists and researchers are increasingly challenging public health orthodoxies on FDA-approved COVID-19 treatments and vaccines. A peer-reviewed paper published in Nature on Monday confirmed that Merck’s COVID-19 pill Molnupiravir causes viral mutations that can lead to new variants. Meanwhile, scientists have been raising the alarm about the Pfizer and…

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Scientists ‘shocked’ and ‘alarmed’ at what’s in the mRNA shots

Early in 2023, genomics scientist Kevin McKernan made an accidental discovery. While running an experiment in his Boston lab, McKernan used some vials of mRNA Pfizer and Moderna Covid vaccines as controls. He was ‘shocked’ to find that they were allegedly contaminated with tiny fragments of plasma DNA. McKernan, who…

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EnGen Lba Cas12a (Cpf1) | NEB

EnGen® Lba Cas12a from Lachnospiraceae bacterium ND2006 is a site-specific DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) (1). Targeting requires a gRNA complementary to the target site as well as a 5’ TTTV protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage…

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CRISPR library screening to develop HEK293-derived cell lines with improved lentiviral vector titers

doi: 10.3389/fgeed.2023.1218328. eCollection 2023. Affiliations Expand Affiliation 1 Division of Cellular and Gene Therapies, Center for Biologics Evaluation and Research, U.S. Food and Drug Administration, Silver Spring, MD, United States. Free PMC article Item in Clipboard Brian J Iaffaldano et al. Front Genome Ed. 2023. Free PMC article Show details Display…

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Contaminated COVID Products and Monkey Virus DNA

Recent research has revealed that in addition to RNA, COVID vaccines also contain DNA fragments, which should not be present and indicate a contamination problem. This discovery has also exposed the proprietary blueprint for RNA production. Microbiologist Kevin McKernan and his team have identified SV40 promoters in the bivalent mRNA…

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Gene Set – RAP2A

Symbol Name ABI2 abl-interactor 2 ADO 2-aminoethanethiol (cysteamine) dioxygenase CAND1 cullin-associated and neddylation-dissociated 1 CCDC88A coiled-coil domain containing 88A CEP350 centrosomal protein 350kDa CLASP2 cytoplasmic linker associated protein 2 CLEC16A C-type lectin domain family 16, member A ENAH enabled homolog (Drosophila) FBXW11 F-box and WD repeat domain containing 11 GABBR2…

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Claim that COVID-19 mRNA vaccines contain DNA contaminants based on study of vials of “unknown provenance”; no evidence COVID-19 mRNA vaccines can alter DNA in people

CLAIM DNA in COVID mRNA shots “may have the ability to alter the human genome”; “SV40 has been linked to cancer in humans”; “Regulatory Agencies Knew There Was a Contamination Problem” DETAILS Misleading: While SV40 is known to cause cancer in certain animals like hamsters, epidemiological studies didn’t find an…

Continue Reading Claim that COVID-19 mRNA vaccines contain DNA contaminants based on study of vials of “unknown provenance”; no evidence COVID-19 mRNA vaccines can alter DNA in people

Development of human cell-based production platform for therapeutic proteins using site-specific integration

Human cell lines, such as human embryonic kidney (HEK) 293, HT-1080, and PER.C6 have been used for the production of therapeutic proteins because of human-like post-translational modifications (PTMs), thereby ensuring proper function of recombinant proteins and eliminating the risk of immunogenic reactions. A traditional strategy for cell line development has…

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Fact Check: Facebook posts – No, the COVID-19 vaccines don’t contain ‘monkey virus DNA’

Social media users are claiming a dangerous ingredient has been found in COVID-19 vaccines. The claim comes from a June 11 Epoch Times article headlined, “Monkey virus DNA found in COVID-19 shots.” A Facebook post that shared the article June 12 said, “Does this amount to genocide perpetrated by Bill…

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No, the COVID-19 vaccines don’t contain ‘monkey virus DNA’

Social media users are claiming a dangerous ingredient has been found in COVID-19 vaccines. The claim comes from a June 11 Epoch Times article headlined, “Monkey virus DNA found in COVID-19 shots.” A Facebook post that shared the article June 12 said, “Does this amount to genocide perpetrated by Bill…

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No, ‘monkey virus DNA’ was not found in COVID vaccines

CLAIM: Vaccines developed for COVID-19 contain a cancer-causing virus DNA found in monkeys. AP’S ASSESSMENT: False. Public health officials and the lead researcher of a study cited in many of the social media posts say there’s no monkey virus DNA in the inoculations approved by government regulators. Some COVID-19 vaccines…

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Fact Check: NO Peer-Reviewed Evidence ‘Green Monkey DNA’ Found In mRNA Vaccines Can Cause Cancer

No Peer Review Did a legitimate, peer-reviewed study successfully demonstrate that COVID-19 mRNA vaccines produced by Pfizer and Moderna were shown to contain simian virus 40 (SV40), a DNA virus that has been linked to certain cancers? No, that’s not true: The research in question was not published in a…

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Nuclear permeability during cell division

In late May I had the honor of speaking with Sucharit Bhakdi, MD regarding dsDNA contamination in the vaccines. First, we need to do some numbers. The 100ug Moderna shots deliver over 40 trillion mRNA molecules (14T for Pfizer) and the number of LNPs per shot is reported to be…

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Oncogenic Transformation Drives DNA Methylation Loss and Transcriptional Activation of Transposable Element Loci | Cancer Research

Transposable elements (TEs) are typically silenced by DNA methylation and repressive histone modifications in differentiated healthy human tissues. However, TE expression increases in a wide range of cancers and is correlated with global hypomethylation of cancer genomes. We assessed expression and DNA methylation of TEs in fibroblast cells that were…

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A Sticky Situation: Recombinant DNA Technology | TS Digest

In September 1970, Janet Mertz joined the biochemistry department at Stanford University. She was one of only six new graduate students in the department and the first female one admitted in nine years. “I wanted to study viruses as I had been working with bacteriophage lambda during my undergraduate studies….

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A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor

Antisense vector design to accommodate independent promoters Here we sought to optimize lentivirus vector-mediated independent co-expression of two genes in transduced human T cells, with one gene under a constitutive promoter and the other under an inducible promoter, to improve adoptive T-cell transfer (ACT) of cancer. We began by building…

Continue Reading A lentiviral vector for the production of T cells with an inducible transgene and a constitutively expressed tumour-targeting receptor

Reprogramming of human peripheral blood mononuclear cells into induced mesenchymal stromal cells using non-integrating vectors

OCT4 alone was insufficient to reprogram PBMCs into iMSCs directly Previously, we reported that lentivirally expressed OCT4 could directly reprogram human cord blood CD34+ hematopoietic progenitor cells into iMSCs with very high efficiency11. Therefore, we first tried to convert human PBMCs into iMSCs by overexpressing OCT4 alone using a clinically…

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Efficient CRISPR-Cas13d-Based Antiviral Strategy to Combat SARS-CoV-2

Affiliations Expand Affiliations 1 Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. 2 Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain. Free PMC article Item in…

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Paul Berg obituary | Genetics

The Oxford-AstraZeneca vaccine against Covid-19 was built on the principle of stitching together DNA from two viruses, one to enable the vaccine to enter cells and the other to provoke an immune response. In 1972 Paul Berg, who has died aged 96, became the first person to combine the DNA…

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ISC Class 12 Biotechnology Syllabus 2022

ISC Class 12th Biotechnology Syllabus 2023: Biotechnology is a field of science that applies biological processes, organisms, or systems to develop products or technologies that improve human lives. It involves using living organisms or their derivatives to create new products or processes. Biotechnology has multiple applications in various areas such…

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Viruses | Free Full-Text | Efficient CRISPR-Cas13d-Based Antiviral Strategy to Combat SARS-CoV-2

Figure 1. Replication cycle of SARS-CoV-2 and genome organization. (A) The SARS-CoV-2 replication cycle. Binding of the viral S protein to the ACE-2 receptor triggers SARS-CoV-2 infection and the plus genomic RNA (+gRNA) is translated into polyprotein (pp)1a and pp1ab. These proteins are proteolytically cleaved to generate 16 non-structural proteins…

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Penk Targeted Allele Detail MGI Mouse (MGI:5501235)

Penktm1.1(cre/ERT2)HzeTargeted Allele Detail Symbol: Penktm1.1(cre/ERT2)Hze Name: preproenkephalin; targeted mutation 1.1, Hongkui Zeng MGI ID: MGI:5501235 Synonyms: Penk-2A-CreERT2, Penk-2A-CreERT2-DeltaNeo Gene: Penk  Location: Chr4:4133536-4138815 bp, – strand  Genetic Position: Chr4, 2.31 cM Alliance: Penktm1.1(cre/ERT2)Hze page Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Mutation details: A targeting vector…

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MONOCLONAL ANTI-FBXW7 | Merck Life Science Indonesia

Description Monoclonal Anti-FBXW7 antibody produced in mouse has been used in Western blotting and immunohistochemistry. F-box proteins are associated with various signaling pathways, such as nutrient sensing in yeast, conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch,…

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Tac2 Targeted Allele Detail MGI Mouse (MGI:5484637)

Tac2tm1.1(cre)HzeTargeted Allele Detail Symbol: Tac2tm1.1(cre)Hze Name: tachykinin 2; targeted mutation 1.1, Hongkui Zeng MGI ID: MGI:5484637 Synonyms: Tac2-IRES2-Cre, Tac2-IRES2-Cre-Delta, Tac2-IRES2-Cre-DeltaNeo/Hygro Gene: Tac2  Location: Chr10:127560347-127567637 bp, + strand  Genetic Position: Chr10, 74.81 cM, cytoband D3 Alliance: Tac2tm1.1(cre)Hze page Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Mutation details: The targeting vector contained, from…

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VectorBuilder | Revolutionizing Gene Delivery.

RSV promoter: Rous sarcoma virus promoter. It drives transcription of viral RNA in packaging cells. This RNA is then packaged into live virus. Δ5′ LTR: A deleted version of the HIV-1 5′ long terminal repeat. In wildtype lentivirus, 5′ LTR and 3′ LTR are essentially identical in sequence. They reside…

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Engineering and validation of a dual luciferase reporter system for quantitative and systematic assessment of regulatory sequences in Chinese hamster ovary cells

Experimental workflow and vector designs One way to validate the all-in-one reporter system is through transient or stable transfection of mammalian cells. Within this framework, the schematics in Fig. 1 illustrate the overall workflow of this study and feature the characteristics of promoters. To summarize, the study involves the engineering and…

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Addressing the Challenges of AAV Production

Adeno-associated virus (AAV) vectors are a versatile and appealing gene therapy delivery platform, capable of targeting a wide range of cell types. However, AAV must be produced in a living system, and issues with scalability and high production costs have so far limited the widespread adoption of the technology. Technology…

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VARIASI GENETIK KULTIVAR STROBERI (Fragaria spp.) BERDASARKAN RFLP MUHAMMAD FAUZI ARIF, Ganies Riza Aristya, S.Si., M.Sc.

1 DAFTAR PUSTAKA Anonim ACRA (Australian Cultivar Registration Authority) Australian National Botanic Gardens Cultivar. cultivar.html. Diakses pada 16 Maret 2017 Aristya, G.R Pewarisan dan Pemetaan Penanda Sequence Characterized Amplified Region (SCAR) Terpaut Gen Penyandi Ketahanan Power Mildew [(Podosphaera xanthii (Castag.) Braun et Shiskoff)] Pada Tanaman Melon (Cucumis melo L.). Tesis….

Continue Reading VARIASI GENETIK KULTIVAR STROBERI (Fragaria spp.) BERDASARKAN RFLP MUHAMMAD FAUZI ARIF, Ganies Riza Aristya, S.Si., M.Sc.