Tag: SV40

No Peer Review Did a legitimate, peer-reviewed study successfully demonstrate that COVID-19 mRNA vaccines produced by Pfizer and Moderna were shown to contain simian virus 40 (SV40), a DNA virus that has been linked to certain cancers? No, that’s not true: The research in question was not published in a…

In late May I had the honor of speaking with Sucharit Bhakdi, MD regarding dsDNA contamination in the vaccines. First, we need to do some numbers. The 100ug Moderna shots deliver over 40 trillion mRNA molecules (14T for Pfizer) and the number of LNPs per shot is reported to be…

Transposable elements (TEs) are typically silenced by DNA methylation and repressive histone modifications in differentiated healthy human tissues. However, TE expression increases in a wide range of cancers and is correlated with global hypomethylation of cancer genomes. We assessed expression and DNA methylation of TEs in fibroblast cells that were…

In September 1970, Janet Mertz joined the biochemistry department at Stanford University. She was one of only six new graduate students in the department and the first female one admitted in nine years. “I wanted to study viruses as I had been working with bacteriophage lambda during my undergraduate studies….

Antisense vector design to accommodate independent promoters Here we sought to optimize lentivirus vector-mediated independent co-expression of two genes in transduced human T cells, with one gene under a constitutive promoter and the other under an inducible promoter, to improve adoptive T-cell transfer (ACT) of cancer. We began by building…

OCT4 alone was insufficient to reprogram PBMCs into iMSCs directly Previously, we reported that lentivirally expressed OCT4 could directly reprogram human cord blood CD34+ hematopoietic progenitor cells into iMSCs with very high efficiency11. Therefore, we first tried to convert human PBMCs into iMSCs by overexpressing OCT4 alone using a clinically…

Affiliations Expand Affiliations 1 Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. 2 Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain. Free PMC article Item in…

The Oxford-AstraZeneca vaccine against Covid-19 was built on the principle of stitching together DNA from two viruses, one to enable the vaccine to enter cells and the other to provoke an immune response. In 1972 Paul Berg, who has died aged 96, became the first person to combine the DNA…

ISC Class 12th Biotechnology Syllabus 2023: Biotechnology is a field of science that applies biological processes, organisms, or systems to develop products or technologies that improve human lives. It involves using living organisms or their derivatives to create new products or processes. Biotechnology has multiple applications in various areas such…

Figure 1. Replication cycle of SARS-CoV-2 and genome organization. (A) The SARS-CoV-2 replication cycle. Binding of the viral S protein to the ACE-2 receptor triggers SARS-CoV-2 infection and the plus genomic RNA (+gRNA) is translated into polyprotein (pp)1a and pp1ab. These proteins are proteolytically cleaved to generate 16 non-structural proteins…

Penktm1.1(cre/ERT2)HzeTargeted Allele Detail Symbol: Penktm1.1(cre/ERT2)Hze Name: preproenkephalin; targeted mutation 1.1, Hongkui Zeng MGI ID: MGI:5501235 Synonyms: Penk-2A-CreERT2, Penk-2A-CreERT2-DeltaNeo Gene: Penk  Location: Chr4:4133536-4138815 bp, – strand  Genetic Position: Chr4, 2.31 cM Alliance: Penktm1.1(cre/ERT2)Hze page Allele Type:    Targeted (Inducible, Recombinase) Inducer:    tamoxifen Mutations:    Insertion, Intragenic deletion   Mutation details: A targeting vector…

Description Monoclonal Anti-FBXW7 antibody produced in mouse has been used in Western blotting and immunohistochemistry. F-box proteins are associated with various signaling pathways, such as nutrient sensing in yeast, conserved developmental pathways in plants and animals. These proteins mediate recognition of phosphorylated targets including Cyclin E, Myc, c-Jun, and Notch,…

Tac2tm1.1(cre)HzeTargeted Allele Detail Symbol: Tac2tm1.1(cre)Hze Name: tachykinin 2; targeted mutation 1.1, Hongkui Zeng MGI ID: MGI:5484637 Synonyms: Tac2-IRES2-Cre, Tac2-IRES2-Cre-Delta, Tac2-IRES2-Cre-DeltaNeo/Hygro Gene: Tac2  Location: Chr10:127560347-127567637 bp, + strand  Genetic Position: Chr10, 74.81 cM, cytoband D3 Alliance: Tac2tm1.1(cre)Hze page Allele Type:    Targeted (Recombinase) Mutation:    Insertion   Mutation details: The targeting vector contained, from…

RSV promoter: Rous sarcoma virus promoter. It drives transcription of viral RNA in packaging cells. This RNA is then packaged into live virus. Δ5′ LTR: A deleted version of the HIV-1 5′ long terminal repeat. In wildtype lentivirus, 5′ LTR and 3′ LTR are essentially identical in sequence. They reside…

Experimental workflow and vector designs One way to validate the all-in-one reporter system is through transient or stable transfection of mammalian cells. Within this framework, the schematics in Fig. 1 illustrate the overall workflow of this study and feature the characteristics of promoters. To summarize, the study involves the engineering and…

Adeno-associated virus (AAV) vectors are a versatile and appealing gene therapy delivery platform, capable of targeting a wide range of cell types. However, AAV must be produced in a living system, and issues with scalability and high production costs have so far limited the widespread adoption of the technology. Technology…

1 DAFTAR PUSTAKA Anonim ACRA (Australian Cultivar Registration Authority) Australian National Botanic Gardens Cultivar. cultivar.html. Diakses pada 16 Maret 2017 Aristya, G.R Pewarisan dan Pemetaan Penanda Sequence Characterized Amplified Region (SCAR) Terpaut Gen Penyandi Ketahanan Power Mildew [(Podosphaera xanthii (Castag.) Braun et Shiskoff)] Pada Tanaman Melon (Cucumis melo L.). Tesis….