Tag: UTP

mRNA preparation The DNA template for the mRNA vaccine comprised a DNA fragment encoding the SFTSV Gn protein. The DNA templates were cloned into a plasmid vector with backbone sequence elements (T7 promoter, 5′- and 3′-UTR, 100 nucleotide poly(A) tail) interrupted by a linker (A50LA50, 20 nucleotides) to improve RNA…

Experimental design Primary T cell isolation, expansion, and cell culture Primary T cells were isolated from human peripheral blood mononuclear cells (PBMCs) of healthy donors collected by the Institute for Transfusion Medicine of the University Clinic of Leipzig, Germany, approved by the Ethics Committee of the Faculty of Medicine of…

KEGG T30434: 197056     T30434 (Metagenome): 132913 Help Entry 132913            CDS       T30434                                  Symbol yngB Name UTP–glucose-1-phosphate uridylyltransferase yngB   KO K00963   UTP–glucose-1-phosphate uridylyltransferase [EC:2.7.7.9] Best hit Taxonomy Firmicutes – Others [GN:dho]Taxonomy Pathway 00040   Pentose and glucuronate interconversions 00052   Galactose metabolism 00500   Starch and sucrose metabolism 00520   Amino sugar and nucleotide sugar metabolism 00541  …

EfCdnE synthesizes products cUU and cUC To understand the molecular basis for synthesizing cyclic di-pyrimidines, the substrate specificity of EfCdnE was first accessed by ITC. The binding of AMPcPP and GMPcPP to EfCdnE was too weak to be detected (Supplementary Fig. 1A, B), indicating that EfCdnE prefers pyrimidine nucleotides over purine…

Figure 1. ATP–Ado extracellular metabolism and elicited signaling through adenosinergic receptors. A, Canonical and noncanonical extracellular receptors contributing to the hydrolysis of ATP and other metabolites. In the TME, two different yet partially overlapping pathways mediate the hydrolysis of ATP to adenosine (Ado). In the noncanonical pathway, Ado can be…

The main components of the essential cellular process of eukaryotic ribosome biogenesis are highly conserved from yeast to humans. Among these, the U3 Associated Proteins (UTPs) are a small subunit processome subcomplex that coordinate the first two steps of ribosome biogenesis in transcription and pre-18S processing. While we have identified…

货号： CSB-YP874796HU 规格： 来源： Yeast 其他： 货号： CSB-EP874796HU 规格： 来源： E.coli 其他： 货号：…

KEGG ENZYME: 3.6.1.39     ENZYME: 3.6.1.39 Help Entry EC 3.6.1.39                 Enzyme                                  Name thymidine-triphosphatase;thymidine triphosphate nucleotidohydrolase;dTTPase;deoxythymidine-5′-triphosphatase Class Hydrolases;Acting on acid anhydrides;In phosphorus-containing anhydridesBRITE hierarchy Sysname dTTP nucleotidohydrolase Reaction(IUBMB) dTTP + H2O = dTDP + phosphate [RN:R02095] Reaction(KEGG) Substrate Product Comment Also acts, more slowly, on dUTP and UTP. History EC 3.6.1.39 created 1984…

Significance Hairpin pyrrole-imidazole (Py-Im) polyamides can be programmed to bind a broad repertoire of DNA sequences. Py-Im small molecules can be used to target cancer-specific coding regions and block transcription elongation. This transcription blockage by Py-Im cannot be rescued by transcription elongation factors, such as TFIIS. The mechanism by which…