Tag: utr

Zafrens Plans to Advance Multimodal Drug Discovery Platform with $23M Financing

Although he started out in theoretical physics, Swamy Vijayan, PhD, has worked in the biotechnology industry for most of his professional career. His path included a stint at Illumina and the launch of Omniome, a short read sequencing company that was acquired by Pacific Biosciences in 2021. His next gig…

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An ncRNA transcriptomics-based approach to design siRNA molecules against SARS-CoV-2 double membrane vesicle formation and accessory genes | BMC Infectious Diseases

When compared to the genomes of other RNA viruses, coronaviruses have been found to possess largest genome sizes. They are capable of establishing reservoirs in both human and zoonotic populations, enabling their transmission and circulation among a range of animal hosts, including bats, pangolins, civets, cats, mice, pigs, whales, dogs,…

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Genome-wide characterization and evolutionary analysis of the AP2/ERF gene family in lettuce (Lactuca sativa)

Identification of the AP2/ERF transcription factors in lettuce genome To identify AP2/ERF family genes in lettuce, we queried the lettuce genomic protein database (version 8) using the Pfam model (PF00847) of the AP2 domain. This search led us to discover 223 genes that showed a significant match with the AP2…

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ncRNA | Free Full-Text | Regulation of Macrophage Polarization in Allergy by Noncoding RNAs

1. Introduction Allergies affect millions of people worldwide and are characterized by an excessive type 2 immune response to normally harmless substances, generally known as antigens or allergens, specifically [1,2]. Consequently, this response leads to the development of various allergic symptoms, including asthma, allergic rhinitis, and atopic dermatitis. In the…

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Shotgun Metagenomic Sequencing | Psomagen, Inc.

More Sequencing Possibilities Choose from our wide range of sequencing options — whole genome to single-cell transcriptomics — to support your project. Genomics The most comprehensive way to investigate an entire genome.Get the whole story, including exons, introns, and untranslated regions (UTR). Transciptomics A more complete picture of various disease…

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GYPB Human shRNA Plasmid Kit (Locus ID 2994) Clinisciences

Product Data Locus ID 2994 Synonyms CD235b; GPB; GYP; GYPA; MNS; PAS-3; SS Vector pGFP-C-shLenti E. coli Selection Chloramphenicol (34 ug/ml) Mammalian Cell Selection Puromycin Format Lentiviral plasmids Kit Components GYPB – Human, 4 unique 29mer shRNA constructs in lentiviral GFP vector(Gene ID = 2994). 5µg purified plasmid DNA per…

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Identification of potential riboswitch elements in homo sapiens mRNA 5primeUTR sequences using positive unlabeled machine learning

This is the submission data repository for the manuscript”Identification of potential riboswitch elements in Homo Sapiens mRNA 5’UTR sequences using Positive-Unlabeled machine learning.” Inside this archive are the original data files, analysis, processed data, and ML ensemble predictions used in the manuscript.Abstract:Riboswitches are a class of noncoding RNA structures that…

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Is there a way to query Ensembl to get all 3’UTRs from all species?

Is there a way to query Ensembl to get all 3’UTRs from all species? 1 I am trying to obtain stats on how many 3’UTRs are annotated in Ensembl. I would really like to download the as many annotated 3’UTRs as possible from as many species as possible and find…

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Single-cell RNAseq analysis of spinal locomotor circuitry in larval zebrafish

. 2023 Nov 17:12:RP89338. doi: 10.7554/eLife.89338. Affiliations Expand Affiliation 1 Vollum Institute, Oregon Health & Science University, Portland, United States. Item in Clipboard Jimmy J Kelly et al. Elife. 2023. Show details Display options Display options Format AbstractPubMedPMID . 2023 Nov 17:12:RP89338. doi: 10.7554/eLife.89338. Affiliation 1 Vollum Institute, Oregon Health &…

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When will RNA get its AlphaFold moment? | Nucleic Acids Research

Abstract The protein structure prediction problem has been solved for many types of proteins by AlphaFold. Recently, there has been considerable excitement to build off the success of AlphaFold and predict the 3D structures of RNAs. RNA prediction methods use a variety of techniques, from physics-based to machine learning approaches….

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Domain definition and preliminary functional exploration of the endonuclease NOBP-1 in Strongyloides stercoralis | Parasites & Vectors

Boes J, Slotved HC, Murrell KD, Eriksen L, Roepstorff A, Nansen P, et al. Alternative migration routes of Ascaris suum in the pig. J Parasitol. 2002;88:180–3. Article  CAS  PubMed  Google Scholar  Nikolaou S, Gasser RB. Prospects for exploring molecular developmental processes in Haemonchus contortus. Int J Parasitol. 2006;36:859–68. Article  CAS …

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mRNA vaccine encoding Gn provides protection against severe fever with thrombocytopenia syndrome virus in mice

mRNA preparation The DNA template for the mRNA vaccine comprised a DNA fragment encoding the SFTSV Gn protein. The DNA templates were cloned into a plasmid vector with backbone sequence elements (T7 promoter, 5′- and 3′-UTR, 100 nucleotide poly(A) tail) interrupted by a linker (A50LA50, 20 nucleotides) to improve RNA…

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Design, optimization, production and activity testing of recombinant immunotoxins expressed in plants and plant cells for the treatment of monocytic leukemia

. 2023 Dec;14(1):2244235. doi: 10.1080/21655979.2023.2244235. Affiliations Expand Affiliations 1 Bioprocess Engineering, Fraunhofer Institute for Molecular Biology and Applied Ecology IME, Aachen, Germany. 2 Institute for Molecular Biotechnology, RWTH Aachen University, Aachen, Germany. 3 University of Natural Resources and Life Sciences, Vienna (BOKU), Department of Biotechnology (DBT), Institute of Bioprocess Science…

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Genome-wide epigenetic dynamics during postnatal skeletal muscle growth in Hu sheep

Global changes of the transcriptome during postnatal muscle growth The number of myofibers is thought to remain fixed following birth, muscle fiber hypertrophy growth is the primaryway of muscle growth. The average CSA is generally used to measure the hypertrophy of skeletal muscle fibers. To systematically identify the growth of postnatal…

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Different “Reads Mapped Confidently to Transcriptome” values in scRNA

Hello everyone. My question is about sing-cell RNASeq. I am re-analyzing a scRNA raw data in my lab, which has previously analyzed by seqencing company, i am trying to replicate the results and update/optimize my pipeline. Currently my pipeline is as follows: 1. Creating custom reference I indexed the reference…

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Epitranscriptomic Modifications and How to Detect Them

What is epitranscriptomic modification? RNA modifications, not limited to purine or pyrimidine bases, including N6-methyladenosine (m6A), 1-methyladenosine (m1A), 5-methylcytidine (m5C), 5-hydroxymethylcytidine (hm5C), 5-formylcytidine (f5C), 5-carboxycytidine (ca5C), inosine (I), pseudouridine (Ψ), and 2′-O-methylation (Nm). RNA modifications can structurally alter the pairing of nucleobases, leading to structural rearrangements of RNA and thus…

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Solved your answers in CAPS LOCK with NO SPACES between the

Transcribed image text: your answers in CAPS LOCK with NO SPACES between the nucleotides – e.g. ATGCCGAG…. DO NOTADD 5′ and 3′ to your answer. TGAGGATGAAAGTCACACCGGGGCGCAGTITGGACTTAGATTCTAGTACACGTCCTAGTATTIAACACAGTT complementary strand: b. Using this DNA double helix, express the gene – i.e. determine the resulting polypeptide sequence by using the correct reading frame….

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The role and impact of alternative polyadenylation and miRNA regulation on the expression of the multidrug resistance-associated protein 1 (MRP-1/ABCC1) in epithelial ovarian cancer

Domcke, S. et al. Evaluating cell lines as tumour models by comparison of genomic profiles. Nat. Commun. 4, 2126 (2013). Article  ADS  PubMed  Google Scholar  Köbel, M. et al. Ovarian carcinoma subtypes are different diseases: Implications for biomarker studies. PLoS Med. 5(12), e232 (2008). Article  PubMed  PubMed Central  Google Scholar …

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W2 Ex Genbank-new-answers – 22111 – ExGenbank-new-answers From 22111 Note: numbers in Part 2 and

ExGenbank-new-answers From 22111 Note: numbers in Part 2 and Part 3 are updated on February 7, 2022. Part 1 QUESTION 1.1 a) Inspecting the FEATURE table of the entry reveals that two CDS regions are defined; therefore there are two genes in this entry. As stated on the GenBank hand-out…

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Biological characterization of coronavirus noncanonical transcripts in vitro and in vivo | Virology Journal

Noncanonical coronavirus transcripts were robustly synthesized and could be categorized into seven populations based on differences in sequence elements To determine the amounts and structures of the noncanonical bovine coronavirus (BCoV) transcripts in HRT-18 cells, nanopore direct RNA sequencing was employed. Quantification by read counts revealed that ~ 30% of the total…

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ncRNA | Free Full-Text | Altered Expression of Vitamin D Metabolism Genes and Circulating MicroRNAs in PBMCs of Patients with Type 1 Diabetes: Their Association with Vitamin D Status and Ongoing Islet Autoimmunity

1. Introduction Type 1 diabetes mellitus (T1DM) is a chronic autoimmune disease characterized by the degeneration of beta cells, resulting in insulin deficiency [1]. T1DM incidence is strongly associated with vitamin D deficiency, and 1,25(OH)2D3 can potentially prevent islet cell death and enhance insulin production. Several studies suggest that low…

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Unveiling the biology of defective viral genomes in vitro and in vivo: implications for gene expression and pathogenesis of coronavirus | Virology Journal

The classification, structure, abundance and reproducibility of coronavirus DVGs RNA transcripts other than those encoded by the coronavirus genome are also synthesized during infection [27]. Based on whether they are relevant to transcription regulatory sequence (TRS) (Figure S1A), a sequence motif from which subgenomic mRNAs (sgmRNAs) are synthesized, coronavirus RNA…

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ncRNA | Free Full-Text | The Intergenic Type lncRNA (LINC RNA) Faces in Cancer with In Silico Scope and a Directed Lens to LINC00511: A Step toward ncRNA Precision

CRC Upregulated 153-5p HIF-1 activates LINC00511, targeting HIF-1’s 3-UTR and +ve feedback loop [78] Oncogene Human CRC tissues vs. paired adjacent non-tumor and CRC cell lines HT29, LOVO, SW620, SW480 vs. normal colon epithelial FHC cell line and athymic BALB/c nude mice for implantation – HNF4 promotes LINC00511 transcription, interaction…

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Rapid emergence and transmission of virulence-associated mutations in the oral poliovirus vaccine following vaccination campaigns

Sample collection During a prospective study of OPV shedding following a National Health Week (NHW) vaccination campaign in Veracruz State, Mexico, we collected 15,331 stool samples over 10 weeks from children receiving the OPV vaccine, household contacts, and community members (Fig. 2). In our previous study of OPV shedding and…

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Addgene: shRNA-Ratio-PosCon

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications. For your Materials & Methods section: shRNA-Ratio-PosCon was a gift from Erik Dent (Addgene plasmid # 183553…

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Mitochondrially mediated RNA interference, a retrograde signaling system affecting nuclear gene expression

Andersson DI, Jerlstrom-Hultqvist J, Nasvall J (2015) Evolution of new functions de novo and from preexisting genes. Cold Spring Harb Perspect Biol 7:a017996 Article  PubMed  PubMed Central  Google Scholar  Andrews S (2010) FastQC: a quality control tool for high throughput sequence data. www.bioinformatics.babraham.ac.uk/projects/fastqc/ Auyeung VC, Ulitsky I, McGeary SE, Bartel…

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Guidelines For mRNA Drug Product Manufacturing And Quality Control

By John F. Cipollo, United States Pharmacopeia Messenger RNA (mRNA) is the template for protein synthesis. It serves as an intermediate to transfer the genetic information from DNA in the nucleus to the ribosome sites for translation to protein. It can encode virtually any protein, making it extremely attractive as…

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GTF files from Ensembl Releases 105 and 106 unsorted

There is nothing wrong with these files. Sort (as any GTF): zcat Homo_sapiens.GRCh38.105.gtf.gz \ | awk ‘$1 ~ /^#/ {print $0;next} {print $0 | “sort -k1,1 -k4,4n -k5,5n”}’ \ | bgzip > Homo_sapiens.GRCh38.105_sorted.gtf.gz That having said, if you need the file being strictly coordinate-sorted then you always have to do…

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First report of bovine viral diarrhea virus subgenotypes 1d and 1e in southern Chile | Virology Journal

From the 24 antigenically positive serum samples, 12 amplicons were obtained for sequencing procedures. The low recovery of amplicons is probably due to RNA degradation consecutive to the delay between the first analysis of the samples and the reception of them in our laboratories, what led to repeated frozen-thaw cycles…

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Understanding the Impact of DNA Methylation on Gene Expression in Different Genomic Regions

Hello everyone, I am currently conducting a DNA methylation analysis and have obtained a list of Differentially Methylated Positions (DMPs) and their associated genes. Additionally, I have collected information such as chromosome location and position. In my dataset, the DMPs are categorized into different genomic regions, including promoters, 5′ UTR,…

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The identification and genetic characteristics of Quang Binh virus from field-captured Culex tritaeniorhynchus (Diptera: Culicidae) from Guizhou Province, China | Parasites & Vectors

Mosquito species field collection and composition A total of 32,177 mosquitoes were collected from 15 counties of Guizhou Province, China. These mosquitoes were sampled from various locations, such as pig pens, cattle pens, trees, houses and other places. Among the collection sites, the largest portion of mosquitoes was obtained from…

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Identification and functional validation of SRC and RAPGEF1 as new direct targets of miR-203, involved in regulation of epidermal homeostasis

Keratinocyte and fibroblast cultures Normal human skin was obtained from surgical residues of breast reduction surgery, with the patients’ written informed consent in accordance with the Helsinki Declaration and with Article L. 1243-4 of the French Code of Public Health. Patients’ written informed consents were collected and kept by the…

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Bioinformatics Software Market Demonstrates a Spectacular Growth by key players PerkinElmer, Illumina, Genedata AG, Genomatix Software

PRESS RELEASE Published August 21, 2023 A Latest intelligence report published by AMA Research with title “Bioinformatics Software Market Insights, Forecast to 2028” provides latest updates and strategic steps taken by competition along with growth estimates of market size. The Bioinformatics Software Market report gives clear visions how the research…

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Advances in methylation analysis of liquid biopsy in early cancer detection of colorectal and lung cancer

Study participants Whole blood samples were collected from 327 participants consisting of 102 with colorectal cancer, 99 with lung cancer, and 126 healthy controls. After excluding 6 patients who withdrew consent to participate and two patients with QC-failed samples, the final analysis included 96 patients with colorectal cancer, 95 with…

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UTR Full Form: Types, Importance, Evolutionary

Flanking Regions: UTRs are located at both ends of an mRNA molecule, surrounding the coding sequence. The 5′ UTR, which comes before the coding sequence, and the 3′ UTR, which comes after it, are the two primary forms of UTRs. Regulatory Functions: UTRs are involved in regulating various aspects of…

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Heimdall, an alternative protein issued from a ncRNA related to kappa light chain variable region of immunoglobulins from astrocytes: a new player in neural proteome

The dogma “One gene, one protein” is clearly obsolete since cells use alternative splicing and generate multiple transcripts which are translated into protein isoforms, but also use alternative translation initiation sites (TISs) and termination sites on a given transcript. Alternative open reading frames for individual transcripts give proteins originate from…

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RepeatMasker error when trying to generate repeat sequence distribution pie chart (all code and errors provided)

RepeatMasker error when trying to generate repeat sequence distribution pie chart (all code and errors provided) 0 Hi, Any answers would be highly appreciated as I’ve been stuck on this for a while. I analyze epigenetic data and process DMR lists and I’m currently having issues producing a pie chart…

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Next-generation sequencing provides novel insights into the mechanisms underlying autism and myotonic muscular dystrophy

In a preprint uploaded to the Research Square* server, researchers used a combination of in vivo mice models and next-generation sequencing techniques to elucidate the pathomechanistic connection between autism and myotonic muscular dystrophy type 1. Their results suggest that CTG expansion in the DMPK gene and muscleblind-like factor inhibition induces…

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Cancers | Free Full-Text | CPSF3 Promotes Pre-mRNA Splicing and Prevents CircRNA Cyclization in Hepatocellular Carcinoma

The real effect of CPSF3 on circRNA expression in HCC cells was tested by analyzing the total RNA fractions obtained from CPSF3-KO HepG2 cells, CPSF3-OE Bel7404 cells, and negative control cells using high-throughput sequencing (Supplementary Files S7 and S8). The data obtained showed that CPSF3-KO cells had more types and…

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Alternative splicing events

Hi Ezhil, Leafcutter relies only on splice-junction-spanning reads, which allows it to be computationally very fast and scalable to 1000s of samples. However, because Leafcutter does not include exon or intron coverage information, it is not suitable for detecting intron retention or other splicing events which involve coverage differences –…

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Cas9-mediated knockout of Ndrg2 enhances the regenerative potential of dendritic cells for wound healing

Ndrg2 expression is reduced in tolerogenic DCs To identify potential targets for gene editing in DCs, we compared transcriptomic profiles of treatment induced tolerogenic DCs, which confer a variety of clinical benefits11,12,13,14,15,16,17 with untreated DCs. Bone marrow-derived DCs were cultivated from wild-type (WT) mice (C57/BL6) according to standard protocols25. Vitamin…

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Ribo-On and Ribo-Off tools using a self-cleaving ribozyme allow manipulation of endogenous gene expression in C. elegans

T3H38 ribozyme efficiently cis-cleaves endogenous mRNAs in C. elegans To test whether the T3H38 ribozyme is adaptable for C. elegans, we envisioned that CRISPR/Cas9-mediated knock-in of the active T3H38 ribozyme into the 3’ untranslated region (UTR) of an endogenous gene would lead to ribozyme-mediated self-cleavage and mRNA degradation as the…

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RNA Sequencing- Definition, Principle, Steps, Types, Uses

RNA sequencing is the molecular technique used to identify the order of nucleotide bases (adenine, uracil, guanine, and cytosine) in an RNA molecule. RNA sequencing (RNA-Seq) is a newly developed next-generation sequencing approach to the transcriptome (a complete set of RNA content within a cell including both coding and non-coding…

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Maf File Bar Code

Maf File Bar Code 0 Hi, I am running the GATK Best practices Pipeline over targeted sequence with reference hg19. After running the entire pipeline. I used maftools to view the file and realized that the file has no Tumor_Sample_Barcode. However I have clinical data related to that patient and…

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U827 | Sterile Alpha Motif Domain Containing Protein 11 (SaMD11)

SaMD11 Contains 1 SAM (sterile alpha motif) domain. The sterile alpha motif (SAM) domain is a putative protein interaction module present in a wide variety of proteins involved in many biological processes. The SAM domain that spreads over around 70 residues is found in diverse eukaryotic organisms . SAM domains…

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Study Explores Role of Prostate Cancer Mutations in 3′ Untranslated Regions

NEW YORK— Researchers have unraveled the effects of mutations in the 3′ untranslated regions (UTR) of prostate cancer-related genes on disease progression, mRNA stability, and translation. In a study published in Cell Reports on Friday, the team, led by investigators at the Fred Hutchinson Cancer Center, studied somatic mutations in…

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SORL1 | ALZFORUM

c.-2484C>A Substitution Non-Coding Upstream of 5′ UTR Zhang et al., 2015 c.-1287T>C(SNP 1) Substitution Non-Coding Upstream of 5′ UTR Rogaeva et al., 2007 c.-1204G>T Substitution Non-Coding Upstream of 5′ UTR Zhang et al., 2015 A2G Substitution Substitution | Missense Coding Exon 1 Unknown. Unknown. Holstege et al., 2022 S6T Substitution…

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Circular RNA RSU1 promotes retinal vascular dysfunction by regulating miR-345-3p/TAZ

Patients and samples A total of 103 diabetes mellitus (type 2) patients were recruited according to the criteria from the American Diabetes Association30. Plasma samples were collected from blood of all the patients, and the patients were classified as diabetes mellitus patients without DR (n = 53) and diabetes mellitus patients with…

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5’/3′ RACE method for sequencing the 5′ and 3′ untranslated regions of Zika virus

Akiyama BM, Laurence HM, Massey AR, Costantino DA, Xie X, Yang Y, Shi PY, Nix JC, Beckham JD, Kieft JS (2016) Zika virus produces noncoding RNAs using a multi-pseudoknot structure that confounds a cellular exonuclease. Science 354:1148–1152 Article  CAS  PubMed  PubMed Central  Google Scholar  Alvarez DE, Lodeiro MF, Ludueña SJ,…

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Optimizing mRNA for the discovery of transformative medicines

By Dr. Alexander Zehnder, CEO, CureVac The success of mRNA vaccines in COVID-19 propelled the role of this new modality to the forefront of medicine. Not only are new prophylactic mRNA vaccines for various respiratory diseases advancing through clinical development, but mRNA medicines are now being developed in many wide-ranging…

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mRNA Vaccines for Rapid Response to Biological Threats

COVID-19 demonstrated the enormous dangers posed by biological threats, severely impacting virtually every community in the world. Mitigating these risks in the future is an urgent global imperative. To that end, the National Biodefense Strategy and Implementation Plan, published by the U.S. government in October 2022, outlines goals for countering…

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Extensive diversity in RNA termination and regulation revealed by transcriptome mapping for the Lyme pathogen Borrelia burgdorferi

Global identification of 3′ ends Initial computational predictions of open reading frames (ORFs) in B. burgdorferi38 overlooked elements such as mRNA boundaries, sRNA genes, and translation initiation from non-canonical start codons. B. burgdorferi transcription start sites (TSSs) and 5′ processed RNA ends were previously detected using 5′RNA-seq37. Here we sought…

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View genomic variant #0000026722 – MSeqDR-LSDB Mitochondrial Disease Locus Specific Database

View genomic variant #0000026722 Chromosome 9 Allele Unknown Affects function (as reported) Not classified Affects function (by curator) Not classified Type – DNA change (genomic) (Relative to hg19 / GRCh37) g.6605141T>G Published as – GERP – Segregation – DB-ID GLDC_000222 MSCV – dbSNP ID – Frequency – Sources ; clinvar; Reference – Variant remarks – Genetic origin – Variant_disease – Average frequency (large NGS studies) Variant not found in online data…

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Mapping transcript location issue.

Mapping transcript location issue. 0 Hi I have performed target prediction for human sample using miranda tool. From the result obtained i want to check which exact part of transcript is the miRNA binding to (CDS,Exome,UTR..). On checking the mapping coordinates they do not lie in between any exact coordinates…

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ddRAD sequencing based genotyping of six indigenous dairy cattle breeds of India to infer existing genetic diversity and population structure

Quality control, alignment and SNP calling The ddRAD sequencing based genotyping of 58 individuals belonging to six native cattle breeds; Gir, Sahiwal, Tharparkar, Rathi, Red Sindhi and Kankrej cattle with their geographical and ecological distribution (Fig. 1) including the productive purpose, coat colour, representative agroclimatic zone, breeding tract, the geographical co-ordinate…

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Design Principles of a Novel Construct for HBB Gene-Editing and Investigation of Its Gene-Targeting Efficiency in HEK293 Cells

Jaing, T.-H., Chang, T.-Y., Chen, S.-H., Lin, C.-W., Wen, Y.-C., & Chiu, C.-C. (2021). Molecular genetics of β-thalassemia: A narrative review. Medicine, 100(45), e27522. Article  CAS  PubMed  PubMed Central  Google Scholar  Rattananon, P., Anurathapan, U., Bhukhai, K., & Hongeng, S. (2021). The future of gene therapy for transfusion-dependent beta-thalassemia: The…

Continue Reading Design Principles of a Novel Construct for HBB Gene-Editing and Investigation of Its Gene-Targeting Efficiency in HEK293 Cells

Amplifying gene expression with RNA-targeted therapeutics

RNA-targeted NBTs that are currently being used to upregulate gene expression address both transcriptional and translational mechanisms and can be roughly divided into two groups: (1) NBTs that increase mRNA abundance by enhancing transcription or increasing mRNA stability (Fig. 3) and (2) NBTs that optimize translation (Fig. 4). Strategies in the first…

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Human bone marrow-derived mesenchymal stem overexpressing microRNA-124-3p inhibit DLBCL progression by downregulating the NFATc1/cMYC pathway | Stem Cell Research & Therapy

Microarray-based expression analysis and GEO database analysis The DLBCL-related miRNA expression profiles of GSE29493 and GSE40239 were retrieved from the Gene Expression Omnibus (GEO) database (www.ncbi.nlm.nih.gov/geo/). MiR-124’s pan cancer analysis is conducted through online websites (www.picb.ac.cn/dbDEMC/). Bioinformatics predicted NFATc1 as the target of miR-124-3p The miRbase (www.mirbase.org/), miRDB (mirdb.org), and…

Continue Reading Human bone marrow-derived mesenchymal stem overexpressing microRNA-124-3p inhibit DLBCL progression by downregulating the NFATc1/cMYC pathway | Stem Cell Research & Therapy

Solved Each gene has a 5′ UTR and a 3′ UTR. Which of the

Transcribed image text: Each gene has a 5′ UTR and a 3′ UTR. Which of the following statements is TRUE in describing these 5′ UTR and 3 ‘ UTR sequences of a gene? All of these statements are true. 5′ UTR (leader) and 3’ UTR (trailer) sequences like the beginning…

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Long-Read Genome Assembly and Gene Model Annotations for the Rodent Malaria Parasite Plasmodium yoelii 17XNL

Author links open overlay panelMitchell J. Godin 1 ∗, Aswathy Sebastian 2 ∗, Istvan Albert 1 2 #, Scott E. Lindner 1 # Show more doi.org/10.1016/j.jbc.2023.104871Get rights and content Abstract Malaria causes over 600 thousand fatalities each year, with most cases attributed to the human-infectious Plasmodium falciparum species. Many rodent-infectious…

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Single-cell gene and isoform expression analysis reveals signatures of ageing in haematopoietic stem and progenitor cells

Annotation of short-read scRNA-seq data with isoform-level information Using fluorescence-activated cell sorting (FACS), we isolated the Lineage-negative, cKit (Cd117) positive (LK) cell fraction of mouse bone marrow cells, a population containing stem and progenitor cells14 from young (8 weeks old, n = 3) and aged (72+ weeks old, n = 3) mice. We generated…

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Phase 1 trial to model primary, secondary, and tertiary dengue using a monovalent vaccine | BMC Infectious Diseases

Messina JP, Brady OJ, Golding N, Kraemer MUG, Wint GRW, Ray SE, Pigott DM, Shearer FM, Johnson K, Earl L, et al. The current and future global distribution and population at risk of dengue. Nat Microbiol. 2019;4(9):1508–15. Article  CAS  PubMed  PubMed Central  Google Scholar  Zeng Z, Zhan J, Chen L,…

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extracting gencode 3utr and 5utr as part of R pipeline

Hello, I reviewed this link 3utr/5utr extraction from Gencode, I want to change and adapt the code as part of an R pipeline, where the genes$type field is replaced. I tried to adapt the code, so it encompassed all UTRs not just protein coding. working_directory_path <- getwd() library(GenomicRanges) # From…

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Sample GenBank Record / Visual abstracts made easy with Mind the Graph

This page presents an annotated sample GenBank record (accession number U49845) in its GenBank Flat File format. You can check the corresponding alive record for U49845, and seeexamples of other records the show a range of biological features. SITE SCU49845 5028 bp DNA PLN 21-JUN-1999 DEFINITION Saccharomyces cerevisiae TCP1-beta gene,…

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Cumulative effects of weakly repressive regulatory regions in the 3’ UTR maintain PD-1 expression homeostasis in mammals

The PD-1 3’ UTR repressed reporter gene expression by promoting mRNA decay The human PD-1 3’ UTR is made up of 1,174 nucleotides, longer than its coding region (867 nts). To explore the revolutionary relationship of PD-1 3’ UTRs of species, we systematically analyzed the sequence conservation of 21,050 protein-coding…

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Circular mitochondrial-encoded mRNAs are a distinct subpopulation of mitochondrial mRNA in Trypanosoma brucei

Some mitochondrial mRNA are circularized CircTAIL-seq is a technique used to Illumina sequence individual transcript PCR libraries of mitochondrial mRNA tails. The approach captures enough of each molecule’s 5′ and 3′ termini that the presence of editing can be confirmed if necessary17. Library preparation first requires circularizing total RNA with…

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3. Go to the NCBI website

3. Go to the NCBI website (www.ncbi.nlm.nih.gov) and search the Gene database for “Homo sapiens FANCG”. Make use of the information provided in the ‘RefSeg transcripts’, ‘RefSeq proteins’ and ‘RefSeqGene’ links to answer the questions below. a) Provide the chromosomal location of this gene. [1] b) What is the accession…

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Mobile element variation contributes to population-specific genome diversification, gene regulation and disease risk

Development and benchmarking of MEGAnE Accurate variant genotyping is required for statistical genetics. To enable both discovery and accurate MEV genotyping from genomes studied using short reads, we developed a new bioinformatic tool, mobile element genotype analysis environment (MEGAnE; Supplementary Note). Compared to SVs resolved by long reads, MEGAnE discovers…

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BCL2 fusions not detected in RNA level

BCL2 fusions not detected in RNA level 0 Hi all, I’ve designed ~120bp probe panel to capture the target sequences, including BCL2, BCL6, MYC genes. In sequencing data, we found some BCL2+ samples, which had been tested with FISH, were not detected in RNA level. Both StarFusion and FusionCatcher were…

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4. A GenBank-formatted DNA sequence file is presented

Transcribed image text: 4. A GenBank-formatted DNA sequence file is presented below. Assume that this sequence represents the sense strand, starts with the 5’UTR, contains two exons, has a single intron, and ends with the 3 ‘UTR. The protein coding sequences have the following coordinates: 343..561,760..1353 (indicated at the CDS…

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Peptide-encoding mRNA barcodes for the high-throughput in vivo screening of libraries of lipid nanoparticles for mRNA delivery

We reasoned that an ideal screening system for functional mRNA delivery would be model independent so that it could be applied in any preclinical model of disease, would consist of multiple measures of protein production that are each orthogonal to any others such that multiple formulations could be tested within…

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Annotate CDS and UTR given transcript

Annotate CDS and UTR given transcript 1 I am annotating a new genome and I am combining several sources of information for the annotation. It combines a de novo annotation as well as lifting over annotations from closely related species. I have been using GFFCompare (ccb.jhu.edu/software/stringtie/gffcompare.shtml) to merge GFF files….

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mRNA vaccines – just the beginning?

By Dr. Myriam Mendila, chief development officer of CureVac World Immunization Week is April 24-30, and it’s an important week to highlight the action needed and to promote the use of vaccines to protect people around the world, of all ages, against infectious diseases. Dr Myriam Mendila, chief development officer,…

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CareDx Showcases Latest Advanc – GuruFocus.com

CareDx, Inc. (Nasdaq: CDNA), a leading precision medicine company focused on the discovery, development, and commercialization of clinically differentiated, high-value healthcare solutions for transplant patients and caregivers – today announced that it will showcase the latest developments across its AlloSeq®* portfolioduring the 36th European Immunogenetics and Histocompatibility Conference taking place…

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Establishing the basis for mammalian intracellular computing with mRNA switches

Credit: Kyoto University Professor Hirohide Saito and his research team have developed a new approach to control mRNA translation using Cas proteins, thus expanding the variety of mRNA switches and making it easier to build synthetic gene circuits in mammalian cells. The results of this research were published in Nature…

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Extract Reads From A Bam file according to gene components `5’UTR, 1st exon, 1st intron, …, 3’UTR, intergenic, …`

Extract Reads From A Bam file according to gene components `5’UTR, 1st exon, 1st intron, …, 3’UTR, intergenic, …` 0 Hello: I mapped the reads in fastq file of bulk RNA seq data to reference genome and get the bam file. My samples have a gene mutation that can induce…

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What does the IGV refseq genes with three different thicknesses of lines mean?

The thinkness indicates whether something is an intron, a coding exon or a UTR. The very thin lines are introns, the full thickness boxes are coding exons and the thinner boxes are UTRs. UTRs, untranslated regions, are exons, they are included in the final mRNA, but they don’t code for…

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Association of SLC11A1 polymorphisms with anthropometric and biochemical parameters describing Type 2 Diabetes Mellitus

Cole, J. B. & Florez, J. C. Genetics of diabetes mellitus and diabetes complications. Nat. Rev. Nephrol. 16, 377–390 (2020). Article  PubMed  PubMed Central  Google Scholar  International Diabetes Federation. IDF Diabetes Atlas 9th edn. (International Diabetes Federation, 2023). Google Scholar  Hu, F. B. Globalization of diabetes: The role of diet,…

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NM_000018.4(ACADVL):c.204G>A (p.Ala68=) AND Very long chain acyl-CoA dehydrogenase deficiency – ClinVar

NM_000018.4(ACADVL):c.204G>A (p.Ala68=) AND Very long chain acyl-CoA dehydrogenase deficiency Based on: 1 submission [Details] Record status: current Accession: RCV001989056.2 Allele description [Variation Report for NM_000018.4(ACADVL):c.204G>A (p.Ala68=)] NM_000018.4(ACADVL):c.204G>A (p.Ala68=) Gene: ACADVL:acyl-CoA dehydrogenase very long chain [Gene – OMIM – HGNC] Variant type: single nucleotide variant Cytogenetic location: 17p13.1 Genomic location: Preferred…

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High-resolution Nanopore methylome-maps reveal random hyper-methylation at CpG-poor regions as driver of chemoresistance in leukemias

Nanopore reads coupled to a novel computational method extends analyses of differential methylation to sparse CpGs (outside CpG islands) We analyzed tumor samples at diagnosis (T) and relapse (R) from three AML patients (UD5, UD10 and AML2) who received standard chemotherapy and relapsed with chemoresistant disease (supplementary Table 1). DNA from…

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ncRNA | Free Full-Text | CircRNAs and RNA-Binding Proteins Involved in the Pathogenesis of Cancers or Central Nervous System Disorders

1. Introduction Many studies have identified some non-coding RNAs (ncRNAs) with abnormal expressions in several diseases and/or disorders [1]. Among them, circular RNAs (circRNAs) are a special type of endogenous ncRNAs, probably formed by back-splicing events, which have attracted more and more interest nowadays. CircRNAs are closed loop structures [2],…

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Solved 1. Genes ___________________________. (more than one

1. Genes ___________________________. (more than one possible answer) A. include start codons B. include promoter and enhancer regions of variable length, which control expression patterns C. often include 5’ and 3’ UTR sequences D. may include variable numbers of introns and exons E. are composed of amino acids 2. Orthologues…

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IJMS | Free Full-Text | Assessment of BMP7, SMAD4, and CDH1 Expression Profile and Regulatory miRNA-542-3p in Eutopic and Ectopic Endometrium of Women with Endometriosis

1. Introduction Endometriosis is one of the most common gynecological diseases, affecting 5–10% of women of reproductive age [1]. It is characterized by the presence of endometrial-like tissue outside the uterine cavity, which is manifested by chronic pelvic pain and/or infertility in 35–50% of women [2,3,4]. The course of this…

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Within-host genetic diversity of SARS-CoV-2 lineages in unvaccinated and vaccinated individuals

Diversity of within-host mutations in SARS-CoV-2 samples We analysed SARS-CoV-2 samples from 2,820 different individuals, alongside 29 other samples for quality control of the analysis (see Methods). The samples were collected from June-2020 to September-2022, covering the third to the fifth COVID-19 waves in HK. All samples had \(\ge\)90% genome…

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Efficient CRISPR-Cas13d-Based Antiviral Strategy to Combat SARS-CoV-2

Affiliations Expand Affiliations 1 Laboratory of Experimental Virology, Department of Medical Microbiology, Amsterdam UMC, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands. 2 Department of Molecular and Cell Biology, National Center of Biotechnology (CNB-CSIC), Campus Universidad Autónoma de Madrid, 28049 Madrid, Spain. Free PMC article Item in…

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Why not use ONLY promoter-bound peaks when testing for enrichment in differentially-bound regions?

In several manuals (example) on ChIP-seq analysis they pre-select, for instance +1000bp and -1000bp from the TSS as the “promoter-bound” regions: peakAnno_bcl11b <- ChIPseeker::annotatePeak(peak = ‘bcl11b_peaks.narrowPeak’, TxDb=txdb, tssRegion=c(-1000, 1000) ) which produces an object with a slot @anno in which each peak is assigned either “Promoter”, “5’ UTR”, “3’ UTR”,…

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The RRM-mediated RNA binding activity in T. brucei RAP1 is essential for VSG monoallelic expression

TbRAP1 interacts with the active VSG RNA in vivo RAP1 homologs have been identified from kinetoplastids to mammals21. None of the known RAP1 homologs has been reported to have any RNA binding activity. We previously found that TbRAP1 does not bind the telomeric repeat-containing RNA (TERRA) in RNA IP experiments22,23,24….

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Pan-genome inversion index reveals evolutionary insights into the subpopulation structure of Asian rice

The 18-genome data package To investigate the genome inversion landscape of Asian rice from a population structure perspective, we first combined a set of 16 previously published high-quality genomes32,33,34 that represent the K = 15 population structure of O. sativa, plus the largest Xian/indica (XI) admixed subpopulation (XI-adm: Minghui 63 (MH63)) to…

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getting coding exon information from Ensembl API

getting coding exon information from Ensembl API 0 hello ! how can i know if an exon is coding or not (includes the utr). can i get this information using API Ensembl ? Thank you API Esembl • 62 views • link updated 47 minutes ago by Ram 38k •…

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Rickettsial DNA and a trans-splicing rRNA group I intron in the unorthodox mitogenome of the fern Haplopteris ensiformis

Our choice of the shoestring fern H. ensiformis as a leptosporangiate fern for complete assembly of both organelle genomes was based on pronounced variability in mitochondrial RNA editing and intron (co-)evolution in the monilophyte family Pteridaceae and the sub-family Vittarioideae in particular22. We used next-generation sequencing (NGS) data to assemble…

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TCGA Methylation Data and Gene Mapping

TCGA Methylation Data and Gene Mapping 0 I am looking into the TCGA Methylation data and I wanted to understand how to parse the data, and, ideally, map measured beta values to single Hugo symbols. My issues are as follows: 1) For some of the Stable Entity IDs there are…

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MiR-223-3p regulates the eosinophil degranulation and enhances the inflammation in allergic rhinitis by targeting FBXW7

Objectives: MiR-223-3p is a multifunctional microRNA regulated by multiple transcription factors and plays a critical role in inflammation. This paper was designed to investigate the regulatory role and mechanism of miR-223-3p in eosinophils degranulation and allergic rhinitis (AR) inflammation. Methods: OVA sensitized AR mouse model and EOL-1 cells model were…

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How to get human cDNA sequences together with UTR regions?

How to get human cDNA sequences together with UTR regions? 2 Dear all, I have downloaded the human genome and gtf files from Gencode. Based on these two files I want to generate a fasta file that has cDNA sequences including 5′ and 3′ UTRs for protein-coding genes only. What…

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Using array variable within loop for two variables awk

This question already has answers here: Closed 18 hours ago. I have an array with few variables for a loop, I want awk to check against one variable, and another persistent string. my script looks like this: wget ftp.ensembl.org/pub/release-109/gtf/homo_sapiens/Homo_sapiens.GRCh38.109.gtf.gz gunzip Homo_sapiens.GRCh38.109.gtf.gz declare -a arr=(“gene” “exon” “transcript” “three_prime_utr”…

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Problem with homer in findMotifs.pl when using input and bg fasta

I am using the following command: findMotifs.pl input.fa fasta ./Output -fastaBg bg.fa -len 8,10,12 -norevopp The input and bg fasta have this structure and the bg fasta include all sequences in input plus many others: >ENSMUST00000027125_Coq10b_mmu_chr1_55071635_55072702_+_utr_55071803_55072702(+) ATTTCTTTTGAATTCCGCTCCCTTCTGCACTCTCAGCTCGCTACTCTGTTCTTCGATGAAGTTGTGAAACAAATGGTAGC AGCCTTTGAAAGAAGAGCCTGTAAACTGTATGGTCCAGAGACAAACATACCTCGGGAATTAATGCTTCATGAAATTCACC ACACCTAAGAGGAAAATATTAGCTGCCTCCACCTACTCTTGGCTAGTTTGTTCACTTCTAGGAAGTCCTTTTACCATCTG` TTGAGAAGTCAGAAAGCATTTGTTAAACCTGCCTTGATTCTAAGCCCGTGCTGTTGAAAATTTGCACATTGAACATGGAC CCACTTGTACATAGAATTATTTCTTCAATCAAGTGTGACTCTAAGTATCATGTACATTTGCAGGCTCCGACCACCTTTGT AATAACGGATGTCATCACTGTTGCTAGGATACCACATTCCTCGTTTGAGTGTACAGATGAACAAGTCTTTTAATTCTCAC CTTACATGAAAAGGTTAGCTGAGATACAATGTGTGTTATATTAACCATATCATGTTTAAGTTATTAGGTTCAGAGTATTT GTAACTTATTGTTATTCGGCATGCCATATGGCTTAGGGTATTTGAATAATCATATATTTACCATTAAAACTGTGATTTAA AGTATTGCTAATGAAGTCTTAGCACTTTGGGTATTTTAATTGTTCTTATGGGTAGCAGTAGATGATTCAGTGTTGTTGGG However I get the following…

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Multiancestry genomic and transcriptomic analysis of gastric cancer

Bray, F. et al. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J. Clin. 68, 394–424 (2018). Article  PubMed  Google Scholar  Sung, H. et al. Global Cancer Statistics 2020: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers…

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Transcript variants conversion from Ensembl ENST to NM

Transcript variants conversion from Ensembl ENST to NM 0 Hi all, I downloaded the 3’UTR folding dataset of human transcripts from Ensembl. However, the mRNA IDs do not contain the transcript variant (example: NM_001662). This causes problems when I have to combine datasets from different sources, as I won’t be…

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Solved Download the full sequence for TP53 (Gencode

Download the full sequence for TP53 (Gencode accession number: ENST00000269305.9) with 500 bp upstream and downstream of the gene. Annotate the 5’ UTR, first four protein coding exons, last protein coding exon, and 3’ UTR. Note, the 5’ and 3’ UTR may be split into multiple exons and if so,…

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Osteoporotic Fracture in Postmenopausal Chinese Females

Introduction Osteoporosis is a frequently diagnosed disease of the bone featured by reduced bone tissue quality as well as a reduced mass of bone tissues. It was determined that > 20 million people suffer from various degrees of osteoporosis at any moment.1,2 Although various therapeutic approaches and agents, such as…

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