Tag: WGCNA

Dementia with Lewy bodies post-mortem brains reveal differentially methylated CpG sites with biomarker potential

Weisman, D. & McKeith, I. Dementia with Lewy Bodies. Semin. Neurol. 27, 042–047 (2007). Article  Google Scholar  Foguem, C. & Manckoundia, P. Lewy Body Disease: Clinical and Pathological “Overlap Syndrome” Between Synucleinopathies (Parkinson Disease) and Tauopathies (Alzheimer Disease). Curr. Neurol. Neurosci. Rep. 2018 18:5 18, 1–9 (2018). CAS  Google Scholar …

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Integrative cross-species analysis of GABAergic neuron cell types and their functions in Alzheimer’s disease

The heterogeneity of GABAergic neurons in human, macaque, mouse, and pig To perform a cross-species comparative study of the GABAergic neurons, we collected the snRNA-seq datasets of the cerebral cortex for human10,11, macaque12,13, mouse14,15, and pig16. After cell-type annotation and filtering out the excitatory neurons and non-neurons, the GABAergic neurons…

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Multi-level cellular and functional annotation of single-cell transcriptomes using scPipeline

Software Figure preparation: CorelDRAW x8 (Corel); Bioinformatic analyses: R v 4.0.3 (R Foundation for Statistical Computing). Computational resources Analyses were run on a desktop computer with an Intel Core i9-10900L CPU (3.70 GHz, 10 cores, 20 threads) with 120 GB RAM running Windows 10 Pro (v21H2). Data preprocessing scRNA-seq data sets…

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Genomic signatures associated with maintenance of genome stability and venom turnover in two parasitoid wasps

Genomic features of two Anastatus wasps, A. japonicus and A. fulloi We employed PacBio high-fidelity (HiFi) long-read sequencing and Illumina short-read sequencing technologies to generate high-quality contigs for two Anastatus wasps, A. japonicus and A. fulloi (Supplementary Tables 1 and 2). These contigs were further scaffolded using Hi-C libraries to…

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rna seq – Tutorial for the WGCNA: changes in heatmap colours

I am trying to reproduce the results of the R tutorial of the WGCNA package. In section I number 5, when generating the heatmap it is quite similar to the one provided by the pdf but the colours of the heatmap differ from the document. Is it possible to change…

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Immune Infiltration and N(6)-Methyladenosine ncRNA Isoform Detection in Acute Lung Injury

Acute lung injury (ALI) is a severe form of sepsis that is associated with a high rate of morbidity and death in critically ill individuals. The emergence of ALI is the result of several factors at work. Case mortality rates might range from 40% to 70%. Researchers have discovered that…

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DESeq2 and WGCNA

DESeq2 and WGCNA 0 I am currently performing an RNAseq analysis with a dataset from a GeneAtlas where I’ve identified DEGs from different comparisons. I want to now do a co-expression analysis with these comparisons and was wondering if anybody had suggestions of tutorials I could be directed to. I…

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Identification of Potential Biomarkers for Progression and Prognosis of Bladder Cancer by Comprehensive Bioinformatics Analysis

This article was originally published here J Oncol. 2022 Apr 19;2022:1802706. doi: 10.1155/2022/1802706. eCollection 2022. ABSTRACT Background. Bladder cancer (BLCA) is a highly malignant tumor that develops in the urinary system. Identification of biomarkers in progression and prognosis is crucial for the treatment of BLCA. BLCA-related differentially expressed genes (DEGs)…

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The modules are not clustered together after WGCNA

The modules are not clustered together after WGCNA 0 I am running WGCNA and trying to visualize the gene network as TOM plot. Rather than using the native function in the package, I am looking for plotting it out by ggplot. The main reason is I would like to also…

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Endometriosis-related functional modules and hub genes

Introduction Endometriosis (EMS) is a chronic gynecological disease defined as implantation and periodic growth of the endometrial glands and stroma outside the uterine cavity, causing chronic pelvic pain, severe dysmenorrhea, and infertility in 10% reproductive-age women, among which the infertility rate is approximately 30–50%.1,2 Surgical excision is commonly used for…

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Workflow Platforms for Systems Genetics

Workflow Platforms for Systems Genetics eQTL Platforms eQTL Viewer Mouse Genome Informatics: Phenotypes, Alleles & Disease Models PhenoGen Informatics (CO U) eXtensible Genotype And Phenotype platform (XGAP) MOLGENIS & MetaNetwork, Swertz & Jansen, U Groningen Weighted Gene Co-expression Network Analysis (WGCNA), Horvath, UCLA iPlant Sage Bionetworks Repository: Synapse GenomeSpace Institute…

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Comprehensive bioinformatics analysis reveals the hub genes and pathways associated with multiple myeloma

This article was originally published here Hematology. 2022 Dec;27(1):280-292. doi: 10.1080/16078454.2022.2040123. ABSTRACT PURPOSE: While the prognosis of multiple myeloma (MM) has significantly improved over the last decade because of new treatment options, it remains incurable. Aetiological explanations and biological targets based on genomics may provide additional help for rational disease…

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Immune microenvironment-related gene mapping predicts immunochemotherapy response and prognosis in diffuse large B-cell lymphoma

doi: 10.1007/s12032-021-01642-3. Affiliations Expand Affiliations 1 Department of Blood Transfusion, Nanfang Hospital, Southern Medical University, Guangzhou, 510515, China. 2 Department of Radiation Oncology, Nanfang Hospital, Southern Medical University, Guangzhou, China. 3 Department of Laboratory Medicine, Nanfang Hospital, Southern Medical University, Guangzhou, China. 4 Department of Blood Transfusion, Nanfang Hospital, Southern…

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Identification of Novel Diagnostic Biomarkers in Prostate Adenocarcinoma Based on the Stromal-Immune Score and Analysis of the WGCNA and ceRNA Network

This article was originally published here Dis Markers. 2022 Jan 15;2022:1909196. doi: 10.1155/2022/1909196. eCollection 2022. ABSTRACT Prostate cancer is still a significant global health burden in the coming decade. Novel biomarkers for detection and prognosis are needed to improve the survival of distant and advanced stage prostate cancer patients. The…

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Image2_Construction and Comprehensive Analysis of a circRNA-miRNA-mRNA Regulatory Network to Reveal the Pathogenesis of Hepatocellular Carcinoma.TIF

Background: Circular RNAs (circRNAs) have been demonstrated to be closely related to the carcinogenesis of human cancer in recent years. However, the molecular mechanism of circRNAs in the pathogenesis of hepatocellular carcinoma (HCC) has not been fully elucidated. We aimed to identify critical circRNAs and explore their potential regulatory network…

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Adrenal aldosterone-producing adenoma | IJGM

Background Primary hyperaldosteronism (PA) is characterized by spontaneous secretion of excessive aldosterone and inhibition of plasma renin activity.1 The pathogenesis of adrenal aldosterone-producing adenoma (APA) involves the abnormal proliferation of adrenal cortex cells and the excessive secretion of aldosterone, accounting for nearly 30% of PA. Excessive secretion of aldosterone can…

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CASP5 and CR1 as potential biomarkers for Kawasaki disease: an Integrated Bioinformatics-Experimental Study

This article was originally published here BMC Pediatr. 2021 Dec 11;21(1):566. doi: 10.1186/s12887-021-03003-5. ABSTRACT BACKGROUND: Kawasaki disease (KD) is a pediatric inflammatory disorder causes coronary artery complications. The disease overlapping manifestations with a set of symptomatically like diseases such as bacterial and viral infections, juvenile idiopathic arthritis, Henoch-Schönlein purpura, infection…

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Biostar WGCNA

Showing : WGCNA • reset 4 days ago • updated 3 days ago wes • 0 12 days ago • updated 10 days ago jms2520 • 0 22 days ago • updated 21 days ago synat.keam • 0 8 weeks ago • updated 7 weeks ago wes • 0 updated…

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Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks

Network analysis was applied to evaluate the association of various ecological microbial communities, such as soil, water and rhizosphere. Presented here is a protocol on how to use the WGCNA algorithm to analyze different co-occurrence networks that may occur in the microbial communities due to different ecological environments. This method…

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After exporting output of WGCNA to VISANT, no network appeared

After exporting output of WGCNA to VISANT, no network appeared 0 Hello I used the WGCNA package to build a network of genes and exported it to Visant for visualization. In VisANT, I first choose clear then choose homo sapiens then file>> open>> however, the screen is still clear nothing…

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Potenial biomarker in Crohn’s diease via bioinformatics

Introduction Crohn’s disease (CD) and ulcerative colitis are chronic inflammatory disorders of the gastrointestinal tract, with symptoms evolving in a relapsing and remitting manner that comprise the term inflammatory bowel disease (IBD).1 CD is characterized by the involvement of all parts of the intestine, the most common being the terminal…

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The Construction and Comprehensive Analysis of a ceRNA Immunoregulator

1Department of Cardiology, Xiangtan Central Hospital, Xiangtan, Hunan, People’s Republic of China; 2Department of Cardiology, Guangxi Cardiovascular Institute, The First Affiliated Hospital of Guangxi Medical University, Guangxi, People’s Republic of China; 3Department of Critical Care Medicine, Zhuzhou Central Hospital, Zhuzhou, Hunan, People’s Republic of China Correspondence: Yiqian ZengDepartment of Critical…

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Trouble with WGCNA gene dendrogram

Trouble with WGCNA gene dendrogram 0 I am trying to plot my gene dendrogram while following the online tutorials for WGCNA. When using the function “table(bwnet$colors)” it shows that there should be 24 modules for my data. When I continue running the code for plotting the dendrogram (code below) I…

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Problem getting soft threshold power using WGCNA for RNA-seq data

Hi jms – soft thresholding (and hard thresholding for that matter) are based on the assumption that use of such thresholds will cut out noise in correlation matrices, thereby “accentuating” the “true” networks in the data. I believe that dozens of empiric experiments substantiate this assumption. Nevertheless, there is still…

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How to read WGCNA edge file output to find the hub genes_ GO ontology

How to read WGCNA edge file output to find the hub genes_ GO ontology 0 Dear Seniors and members, I am getting close to finish my analysis soon, but I would like to ask two more questions. Hope you do not mind me. Question 1 I have WGCNA edge file…

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how to save TOMplot into a pdf/png file

how to save TOMplot into a pdf/png file 1 I am using WGCNA library and wonder how I can write the result of TOMplot into a file which format and function is appropriate. I have used pdf command and dev.off() but the resulting file was empty. R WGCNA • 106…

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WGCNA, analysis on two data sets

WGCNA, analysis on two data sets 1 Hello, I have two healthy and cancer data sets and want to do WGCNA analysis. My goal is to detect the genes that are significant/different in these two data sets comparing to each other. My questions is should I follow the second part…

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Entrez Gene ID

Dear Seniors and all members, Me again!! I hope you do not mind me as a junior in RNAseq and tried to learn and finish my degree. Sorry for another question. I have done WGCNA and was able to identify the module associated with traits and exported data for Gene…

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WGCNA Analysis Identifies Polycystic Ovary Syndrome-Associated Circula

Introduction Polycystic ovary syndrome (PCOS) is a common endocrine metabolic disorder in women of childbearing age.1 PCOS patients are typically characterized by androgen excess, polycystic ovaries and anovulation, and are often accompanied by insulin resistance and obesity.2 Consequently, patients with this syndrome not only suffer from infertility,3 but also have…

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Importing Network From Wgcna Into Cytoscape

Importing Network From Wgcna Into Cytoscape 2 I have performed wgcna analysis and have obtained 2 files for importing the network into cytoscape, one edge file and another node (attribute) file. The edge file has the following columns: fromNode toNode weight direction fromAltName toAltName Os.9416.1.S1at Os.11330.1.S2at 0.1920219249 undirected Os02g33110.1 Os03g28260.1…

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Filtering Gene by mean expression

Filtering Gene by mean expression 0 Dear All, Sorry for another post. I have RNAseq data and used DESeq2 pipeline for differntially expressed genes. Now I am doing WGCNA and the author suggest to filter genes based on mean expression rather than variance. I am wondering whether anyone has ever…

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Genes and Pathways Involved in Postmenopausal Osteoporosis

Introduction Many postmenopausal women suffer from postmenopausal osteoporosis (PMO). A survey of 3247 Italian postmenopausal women found that according to bone mineral density (BMD) diagnostic criteria, the prevalence of osteoporosis was 36.6%.1 PMO patients may suffer from chronic pain and fractures, and as a result their quality of life is…

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Univ of Allahabad Scientists Inch Closer To Interpreting the Stem Cell Code

A colony of human embryonic stem cells (centre) from the H9 cell line. Image: Ryddragyn/Wikimedia Commons, public domain Stem cells are special cells that develop into different cell types in the human body, for example, nerve cells or muscle cells, making them highly desirable candidates for treating diseases. Scientists from…

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Module trait association in wgcna

Module trait association in wgcna 1 Hi all, I am using wgcna for coexpression analysis of time series data. I have 48 samples in total (16 time points with 3 replicates). I have identified different modules based on gene correlation across all samples. After this I want to associate these…

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Exporting WGCNA step-to-step network construction objects into igraph object

Exporting WGCNA step-to-step network construction objects into igraph object 1 Hi, I constructed my weighted network from expression data via step-to-step network construction in WGCNA package. Now, I have an .RData file includes “MEs”, “moduleLabels”, “moduleColors”, “geneTree” and I would like to find an away for exporting this network into…

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Finding whether WGCNA module is associated with different categorical treatment conditions

Hi Seniors and all members, Hope you all are well. My question may be answered somewhere in Biostar, but after reading many posts, it seems to me that it did not solve the problem I am dealing with. My big apology for asking and understand you all are busy, but…

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WNCNA with two factors (with interaction term)

WNCNA with two factors (with interaction term) 0 Hi all! I’m running a WGCNA to correlate gene modules with physiological measurements. My experimental design has two temperatures and three genotypes, and I am interested in the interaction between genotype and temperature. I went through the official tutorial and then the…

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Weird WGCNA plot

Weird WGCNA plot 2 Dear Seniors and All members, I am wondering whether anyone has done weighted gene co-expression network analysis (WGCNA) from Bulk-RNAseq before. I followed the WGNCA tutorial using my data and the module detection outputs are here. However, once I visualized the modules in clustering, I got…

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Exploration of Prognostic Biomarkers of Muscle-Invasive Bladder Cancer (MIBC) by Bioinformatics

This article was originally published here Evol Bioinform Online. 2021 Oct 28;17:11769343211049270. doi: 10.1177/11769343211049270. eCollection 2021. ABSTRACT We aimed to discover prognostic factors of muscle-invasive bladder cancer (MIBC) and investigate their relationship with immune therapies. Online data of MIBC were obtained from The Cancer Genome Atlas (TCGA) and Gene Expression…

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Identification of Hub mRNAs and lncRNAs in Atrial Fibrillation Using Weighted Co-expression Network Analysis With RNA-Seq Data

This article was originally published here Front Cell Dev Biol. 2021 Oct 4;9:722671. doi: 10.3389/fcell.2021.722671. eCollection 2021. ABSTRACT Atrial fibrillation (AF)/paroxysmal AF (PAF) is the main cause of cardiogenic embolism. In recent years, the progression from paroxysmal AF to persistent AF has attracted more and more attention. However, the molecular…

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Severe trauma and burns accompanied by sepsis

Introduction Trauma accounts for approximately 10% of deaths and 16% of disabilities worldwide.1 Billions of dollars have been spent on research into new biological therapeutics for severe injuries, as well as post-traumatic sepsis and septic shock.2 Burn injuries cause unpredictable trauma and sepsis is a complication associated with high morbidity…

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Opposite Signed Genes in WGCNA Signed Consensus Analysis

Hello, I was wondering if anyone could tell me how it would be possible to have genes that have negative correlations (as determined by kME values) with a module in a signed WGCNA analysis. My understanding is that, by definition, all the genes in a module should have a positive…

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how to get ride of duplicated genes when we also have duplicated Ensemble ID in the expression profile?

how to get ride of duplicated genes when we also have duplicated Ensemble ID in the expression profile? 0 Hi all, I have a mouse expression profile that is annotated with gene symbols and many of them are duplicated. I usually use collapseRows function with maxMean method from WGCNA package…

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Study Modules significance (or Modules Preservation) in ONE network

Study Modules significance (or Modules Preservation) in ONE network 1 I am studying only one co-expression network utilizing WGCNA. I would like to validate that the modules identified by WGCNA are significantly above randomly generated modules. 1. What would be the best way to do it? 2. Is there a…

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Identification of Hub Genes Associated With Clear Cell Renal Cell Carcinoma by Integrated Bioinformatics Analysis

This article was originally published here Front Oncol. 2021 Sep 30;11:726655. doi: 10.3389/fonc.2021.726655. eCollection 2021. ABSTRACT BACKGROUND: Clear cell renal cell carcinoma (ccRCC) is a common genitourinary cancer type with a high mortality rate. Due to a diverse range of biochemical alterations and a high level of tumor heterogeneity, it…

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Importing WGCNA edge and node files into Cytoscape

I’ve used the WGCNA packages (horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/) to generate edge and node files for use in Cytoscape. cyt = exportNetworkToCytoscape(modTOM, edgeFile = file.path(“./environment”, paste(label, “_CytoscapeInput-edges-“, paste(modules, collapse=”-“), “.txt”, sep=””)), nodeFile = file.path(“./environment”, paste(label, “_CytoscapeInput-nodes-“, paste(modules, collapse=”-“), “.txt”, sep=””)), weighted = TRUE, threshold = 0.02, nodeNames = modProbes, altNodeNames = modGenes, nodeAttr…

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Combining different data types into a single matrix for WGCNA using DESeq2 normalization

Combining different data types into a single matrix for WGCNA using DESeq2 normalization 0 Hi, I have two different RNA-seq datasets for the sample set of samples, generated using mRNA-seq and smallRNA-seq. The goal here is to identify a set of coding-genes and smallRNAs (no known function/targets) that act together….

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Biocmanager Install Vs Install Packages

Introduction to RNAseq I Day 3 Nicolas Rochette (EEB/ISG, UCLA) Karolina Kaczor-Urbanowicz (Oral Biology & Medicine, UCLA) UCLA Institute for Quantitative and Computational BiologyOver-representation (or enrichment) analysis is a statistical method that determines whether genes from pre-defined sets (ex: those beloging to a specific GO term or KEGG pathway) are…

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WGCNA error during network construction

I am performing WGCNA analysis on my RNAseq dataset and getting this error message: >net = blockwiseModules(expression, power = 6, + TOMType = “unsigned”, minModuleSize = 30, + reassignThreshold = 0, mergeCutHeight = 0.25, + numericLabels = TRUE, pamRespectsDendro = FALSE, + saveTOMs = TRUE, + saveTOMFileBase = “SW_TOM”, +…

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PCA result and batch effect?

PCA result and batch effect? 0 Hello, I am processing a dataframe that consists of about 55000 genes(TPM values,no access to raw data) and 400 samples. After removing the zero variance genes, I am performing a PCA on the samples trying to detect outliers. I have noticed that there are…

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WGCNA Labeled Heatmap

Hi. I’m trying to create a labelled heatmap of module trait relationships using the following code from the WGCNA tutorial: png(filename = “Module-Trait Relationship.png”, width = 20, height = 30, res=300, unit=”cm”) par(mar = c(6, 8.5, 3, 3)) labeledHeatmap(Matrix = moduleTraitCor, xLabels = names(stressdatTraits), yLabels = names(MEs), ySymbols = names(MEs),…

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WGCNA, what does it mean if no hub genes are identified?

WGCNA, what does it mean if no hub genes are identified? 0 I ran WGCNA for my genes following the tutorials by Horvath and Langfelder (horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/). I’ve obtained my modules and the GS and MM for my list of genes – however I think my situation is a bit odd….

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WGCNA

WGCNA 0 0 Entering edit mode 2 hours ago Nithya ▴ 10 Can any one help to solve this Error?? Installation WGCNA • 15 views ADD COMMENT • link 2 hours ago by Nithya ▴ 10 Login before adding your answer. Similar Posts Loading Similar Posts Traffic: 1630 users visited…

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Weird PCA plot based on WGCNA results

Weird PCA plot based on WGCNA results 0 Dear all, I used WGCNA to find the associated gene modules with the different subtypes of a given cancer as trait. I obtained multiple modules associated with some of the cancer subtypes as shown in . Next, I plotted PCA to visualize…

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Community detection protocol and failed gene enrichment

My data is a file of about 19000 genes from a 100 patients. I tried to use these data to create a network by using igraph. Firstly, I had all the names of the genes converted to ENTREZID and from the 19000 genes I kept around 14000. Then I had…

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Apply batch effect with “combat” in wgcna

Apply batch effect with “combat” in wgcna 0 I have 336 samples, with two conditions of fibrosis and normal , and male and female genders , and Chinese, Indian, Malay , Caucasian , Bruneian, and not reported nationalities, I want to apply the batch effect by combat to this study…

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Module preservation analysis using WGCNA

Module preservation analysis using WGCNA 0 Dear Friends, I have the gene expression microarray dataset (about 17000 genes) of about 400 cancer samples with different cancer subtypes (say A, B, C, and D) and about 30 control samples. Here, I used only cancer samples and considered 50% of genes with…

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how to increase modules in WGCNA

how to increase modules in WGCNA 0 Hi, I did wgcna analysis to do the project I used 336 samples for this analysis, but in the end, it gave me 3 modules, about 16,000 genes, 12,000 in one module, 379 in one module, and 212 in another. I think I…

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Regarding finding hub genes using WGCNA

Regarding finding hub genes using WGCNA 1 Dear all, I have got the gene expression microarray dataset (about 17000 genes) of about 400 cancer samples with different cancer subtypes. I considered subtypes as traits (binary traits) and used WGCNA to find the possible modules associated with traits and to identify…

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WGCNA (TRAIT DATA)

WGCNA (TRAIT DATA) 0 I had already obtained a group of significant proteins after performing analysis on data obtained from LC-MS. I would like to perform network analysis on selected proteins using WGCNA package in R. Network analysis using WGCNA:- Removed outlier samples and Genes. Identified softpower (Beta) for singed…

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Getting data from TOM matrix (WGCNA)

Getting data from TOM matrix (WGCNA) 0 Hello, I would like to access the correlation in TOM matrix of a specific subset of genes. Manually, as it is a matrix, there’re no row/column names so I can’t identify the genes I’m looking for. In the manual, I’ve seen the function…

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pre-proccessing of RNAseq data for WGCNA

pre-proccessing of RNAseq data for WGCNA 0 Hi everyone, i wanted to create an expression matrix for WGCNA input. however, i has been said that use RPKM/FPKM data instead of CPM, how can i change my TCGA data to RPKM/FPKM in GDCquery and how to filter expression set of genes…

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WGCNA significant genes

Hi, I am using WGCNA to construct a network and find significant genes for a trait of interest. After making the network, I chose a module based on the pvalue and correlation value. Then I calculated gene significance values for all genes in that module. trait_x <- as.data.frame(datTraits[, “trait_x”, drop…

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WGCNA for diferent stages (I-IV)

WGCNA for diferent stages (I-IV) 0 I wanted to share my issue with WGCNA. any help would be very valuable for me I wanted to know that for identifying modules related with Stage I to Stage IV, how can i binarize these stages into 0, 1, 2, 3, respectively? i…

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Integrated bioinformatics analysis to identify abnormal CC

Introduction In recent years, the morbidity and mortality of colon cancer have increased rapidly, both being ranked fourth worldwide. Although surgery-based comprehensive treatments improve the prognosis of colon cancer, because of the lack of available means for early diagnosis, the mortality level remains high for patients with advanced-stage cancer. The…

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Strategies to learn about a gene of interest from single-cell RNA-seq data

Strategies to learn about a gene of interest from single-cell RNA-seq data 0 Using a large public single-cell RNA-seq dataset from brain where cells are already segregated by brain region, cell type, marker gene cluster, etc. I am looking to do exploratory analyses to learn whatever I can about a…

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How filter genes to construct co-expression network?

How filter genes to construct co-expression network? 1 Hi, I am interested to filter data for constructing co-expression network , Which parameter can i use to filter genes? As i know in WGCNA tutorial, it suggests not to use differential expressed genes(DEG) to filter genes. WGCNA Co-expreesion network DEG Filtering…

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How to load Seurat Object into WGCNA Tutorial Format

As far as I can find, there is only one tutorial about loading Seurat objects into WGCNA (ucdavis-bioinformatics-training.github.io/2019-single-cell-RNA-sequencing-Workshop-UCD_UCSF/scrnaseq_analysis/scRNA_Workshop-PART6.html). I am really new to programming so it’s probably just my inexperience, but I am not sure how to load my Seurat object into a format that works with WGCNA’s tutorials (horvath.genetics.ucla.edu/html/CoexpressionNetwork/Rpackages/WGCNA/Tutorials/)….

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