Tag: Xpert

Direct Detection of Fluoroquinolone Resistance in Sputum Samples from Tuberculosis Patients by High Resolution Melt Curve Analysis

WHO (2022) Global Tuberculosis Report. WHO (2022) WHO consolidated guidelines on tuberculosis, Module 4: Treatment- Drug-resistant tuberculosis treatment: 2022 update. WHO (2020) Meeting report of the WHO expert consultation on the definition of extensively drug-resistant tuberculosis. Jabeen K, Shakoor S, Hasan R (2015) Fluoroquinolone-resistant tuberculosis: implications in settings with weak…

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Genomic characterization of SARS-CoV-2 from Uganda using MinION nanopore sequencing

In this study, we sequenced and characterized 49 SARS-CoV-2 samples from Uganda, collected between April 2020 and July 2021, using MinION Nanopore sequencing. Overall, the ARTIC protocol used was able to generate the required libraries for successful sequencing on the long-read sequencer. MinION Oxford Nanopore technology enabled the identification of…

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Characteristics of Klebsiella pneumoniae co-infections

Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis (M. tuberculosis), is a prominent issue in the field of global public health, particularly in lower-middle-income-countries. Nearly two-thirds of global TB cases come from eight countries, including China.There are 780,000 new TB cases in China every year.1 TB is characterized by increased risk…

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BDQ resistance and molecular characterization of RR-TB

Enyu Tong,1,* Ying Zhou,1,* Zhengwei Liu,2 Yelei Zhu,2 Mingwu Zhang,2 Kunyang Wu,2 Junhang Pan,2 Jianmin Jiang1– 3 1School of Public Health, Hangzhou Normal University, Hangzhou, 311100, People’s Republic of China; 2Tuberculosis Control Department, Zhejiang Provincial Center for Disease Control and Prevention, Hangzhou, 310051, People’s Republic of China; 3Key Laboratory of…

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Detecting drug resistance of Mycobacterium tuberculosis

Introduction According to the World Health Organization report 2022, the incidence rate of tuberculosis in China is 7.4%, with a year-on-year increase of 1.6%. China ranks third globally in terms of tuberculosis cases, with the top three countries being developing nations.1 It is worth noting that the tuberculosis mortality rate…

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Clinical Evaluation of Metagenomic Next-Generation Sequencing and Iden

Introduction Infection is one of the major causes of death in patients with acute and critical illnesses. Especially, lower respiratory tract infections (LRTIs) are the most common infectious diseases worldwide, with a high mortality rate.1,2 As estimated, there are about 10 million new cases of LRTIs in China each year.3,4…

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Cervical screening using HPV-DNA cytology in a low-income setting: an audit within a socio-economically deprived rural community in the Philippines

Cervical cancer (CC) is the fourth most diagnosed cancer and the fourth leading cause of cancer death among women globally.1 In the Philippines, CC is the second leading cause of cancer in women. In 2018 there were 9.1% new CC cases in the Philippines, compared to 0.47% new cases in…

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Global DNA Polymerase Market Size, Trends, Demand, Forecast to 2031

DNA polymerase is a group of enzymes that speeds up DNA synthesis during replication. A cell’s DNA is duplicated during cell division. Nucleotides are added to the 3’OH group to digest the growing DNA strand. It is significant because it facilitates the transmission of genetic information from one generation to…

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Rapid detection of fluoroquinolone resistance in Mycobacterium tuberculosis using a novel multienzyme isothermal rapid assay

Global tuberculosis report 2022. Geneva: World Health Organization; 2022. licence: cc bY-Nc-sa 3.0 iGo. WHO operational handbook on tuberculosis. Module 4: treatment – drug-resistant tuberculosis treatment. Geneva: World Health Organization; 2020. Licence: CC BY-NC-SA 3.0 IGO. Chien JY, Chiu WY, Chien ST, Chiang CJ, Yu CJ, Hsueh PR. Mutations in…

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Drug resistance-associated mutations of M.TB

Introduction Tuberculosis (TB) is one of the most common causes of death and the leading cause of a single infectious agent.1 According to the 2022 Global TB Report of the World Health Organization (WHO), 10.6 million people were infected with TB in 2021, and although the annual number of deaths…

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What is Urinary Tract Tuberculosis?

IntroductionThe problemClinical presentationTypes of Urinary TBComplicationsDiagnosis Management References Further Reading ​​​​​​​​​​​​​​Introduction Urinary tract tuberculosis is usually caused by the bacilli spread through the bloodstream from chronic latent pulmonary tuberculosis. In many cases, the condition’s occurrence goes unrecognized, mistaken for those coincident symptomatic urinary tract infections (UTIs) by other microbes. Tuberculosis (TB) is the…

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Value of mNGS for rapid diagnosis of spinal tuberculosis

Introduction Mycobacterium tuberculosis (MTB) infection in humans leading to tuberculosis (TB), which is an ancient infectious disease that threatens public health.1 TB can be categorized as extrapulmonary TB (EPTB) or pulmonary TB according to the status of MTB infection in the lungs.2 Notably, EPTB is the collective term for all…

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Exploring Tumor Biomarkers in Urothelial Carcinoma: Insights and Advancements in Bladder Cancer Research

Read the Full Video Transcript Sam Chang: Hello everyone. My name is Sam Chang. I’m a urologist in Nashville, Tennessee and work at Vanderbilt University Medical Center. And we’re quite fortunate to have Dr. Chad Ritch from the University of Miami. I’ve known Chad a long time. He’s an associate…

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Soniazid and ethionamide in Mycobacterium tuberculosis

Introduction Tuberculosis (TB), caused by Mycobacterium tuberculosis, is one of the leading causes of death worldwide and caused 1.6 million deaths and 10.6 million new cases in 2021.1 Despite decades of efforts to adopt measures to control TB, achieving the goal of ending TB has been delayed due to a…

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Screening of natural Wolbachia infection in mosquitoes (Diptera: Culicidae) from the Cape Verde islands | Parasites & Vectors

Study area and sample collection An entomological survey was carried out in Cape Verde between February and June 2021. Larval and adult mosquito samples were collected on six islands (Santiago, Brava, Fogo, Maio, Santo Antão, and Boavista; Fig. 1) using BG-Sentinel and Centers for Disease Control and Prevention (CDC) light traps,…

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Digital PCR (dPCR) and Real-time PCR (qPCR) Market worth $12.4 billion

CHICAGO, April 11, 2023 /PRNewswire/ — The dPCR and qPCR industry is expected to experience significant growth in the near future. This is due to the increasing demand for precise, accurate, and efficient detection of nucleic acids for various applications, such as gene expression analysis, disease diagnosis, and food safety testing….

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ST7 becomes one of the most common S. aureus clones

Introduction Staphylococcus aureus (S. aureus), a major human opportunistic pathogen, can cause a variety of infectious diseases including skin and soft-tissue infections, bacteremia, ventilator-associated pneumonia and toxic shock syndrome.1,2 A rapid and proper administration of antibiotics is the most important means to inhibit S. aureus’ growth and prevent disease progression….

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