Tag: zygotes

Jaing, T.-H., Chang, T.-Y., Chen, S.-H., Lin, C.-W., Wen, Y.-C., & Chiu, C.-C. (2021). Molecular genetics of β-thalassemia: A narrative review. Medicine, 100(45), e27522. Article  CAS  PubMed  PubMed Central  Google Scholar  Rattananon, P., Anurathapan, U., Bhukhai, K., & Hongeng, S. (2021). The future of gene therapy for transfusion-dependent beta-thalassemia: The…

doi: 10.1007/978-1-0716-2990-1_11. Affiliations Expand Affiliations 1 Animal Modeling Core, University of Missouri, Columbia, MO, USA. davisdaniel@missouri.edu. 2 Rat Resource and Research Center, University of Missouri, Columbia, MO, USA. 3 Animal Modeling Core, University of Missouri, Columbia, MO, USA. Item in Clipboard Daniel J Davis et al. Methods Mol Biol. 2023. Show…

Genetic/Congenital The intended use of gene editing tools on pre-implantation human embryos would be to avoid the development of congenital diseases in the upcoming baby. But it may have its own risks. Those risks were illustrated in a publication in the March 7, 2023, issue of Nature Communications, where researchers…

Título:  Autor:  Bermejo Álvarez, Pablo; Rizos Dimitrios, Dimitrios; Rath, D.; Lonergan, P.; Gutiérrez Adán, Alfonso Palabras clave:  mtDNAMethylationmRNA transcription Fecha de publicación:  2008 Editor:  American Physiological Society Citación:  Physiological Genomics 32(2): 264-272 (2008) Resumen:  Epigenetic differences between male and female bovine blastocysts provide a plausible link between physiological and gene…

by Prof Riaz A Shah Genome editing in livestock has the potential to bring about significant improvements in productivity, health, and welfare, but there are still challenges that need to be addressed. A SKUAST-K scientist at work The livestock industry is facing a growing demand for animal-based foods to feed…

Animals B6N mice, ICR (CD1) mice, SD rats, and LE rats were obtained from The Jackson Laboratory Japan (Yokohama, Japan). LH rats (No. 0873) were obtained from the National Bio Resource Project for the Rat (NBRP-Rat, Kyoto, Japan). ELKS conditional KO mouse was made floxed ELKS mouse crossed with E3N-Cre…

From protein sequencing to electron microscopy, and from archaeology to astronomy, here are seven technologies that are likely to shake up science in the year ahead. Single-molecule protein sequencing The proteome represents the complete set of proteins made by a cell or organism, and can be deeply informative about health…

Gu, T. P. et al. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 477, 606–610 (2011). Article  CAS  Google Scholar  Wossidlo, M. et al. 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. Nat. Commun. 2, 241 (2011). Article  Google Scholar  Iqbal, K., Jin, S….

PAM identification of NcCas9 by PAM-DOSE The type II-C Cas9 orthologue NcCas9 is of small size (1082 aa) and is closely related to conventional Neisseria meningitidis Cas9 (NmeCas9)11 (Fig. 1a). The NcCas9 is 94% identical to Nme1Cas9, and the divergences lie mainly in the C-terminal PAM-interacting domain (PID) (Fig. 1a and S1)….

Animal generation The Lrsam1C698R knock-in mice were generated on a C57Bl/6J background using the CRISPR/Cas9 technique. Briefly, single-cell zygotes from C57BL/6J mice were microinjected with mRNA encoding Cas9 and a guide sequence (5′-ACAGCAGCAGACGTGGCCAC-3′ at 20 ng/µl) to target the exon 26 of Lrsam1. A single stranded DNA oligo carrying the C698R mutation…

S. cerevisiae cells distinguish between WT and mutant mtDNA. (A) Schematic illustration of the pedigree analysis. Two Δarg8 yeast strains harboring WT or Δcob::ARG8 mtDNA were mated. Zygotes were isolated, and daughter cells from up to five consecutive generations were separated and placed on free spots on the agar plate…

  editing methodologies. It is possible that continuing research may yield new methodologies that rapidly supersede the safety and efficacy of current editing approaches. Non-Heritable Genome Editing: The Use of Genome Editing in Somatic Cells One potential alternative to HHGE for the treatment of genetic dis- eases is somatic genome…

doi: 10.1016/j.mex.2021.101419. eCollection 2021. Affiliations Expand Affiliations 1 Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA. 2 Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA. Free PMC article Item in Clipboard Anil K Challa et al….