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Tag: zygotes
Sickle cell triumph raises questions
In the realm of genetic manipulation, Crispr technology emerges as a double-edged sword. Enabling precise gene editing, it holds promise for eradicating diseases like sickle cell through treatments like Casgevy. Yet, with the power to shape the very fabric of life, ethical concerns loom large. While some foresee a future…
Electroporation of Oct4-specific shRNA expression vectors.
(A) Blastocyst development following electroporation of zygotes with custom Oct4-specific shRNA expression vectors at different concentrations and cultured for 3.5 d. (B) Blastocyst development following electroporation of zygotes with different shRNA expression vectors with a concentration of 100 µg/ml. (C) Morphological appearance of electroporated zygotes obtained from the control and…
New study provides convincing evidence of the essential role of cylicins in sperm development and fertility
In a recent study published in eLife, a group of researchers investigated the Role of Cylicins in Spermiogenesis and Male Fertility in Mice and Humans through Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/ CRISPR-associated protein 9 (Cas9) Gene Editing. Study: Cylicins are a structural component of the sperm calyx being…
The chimaeras of nature and their promise to grow human organs | Explained
In a recent landmark study, scientists have reported successfully producing a live infant chimeric monkey of the species Macaca fascicularis. Representative individual shown above, April 23, 2022. | Photo Credit: Sharp Photography (CC BY-SA 4.0) At present, more than 3 lakh people are waiting for an organ transplant in India…
How one gene shakes up our understanding of male infertility
In a recent article published in the journal Development, researchers investigate the role of the ACTL7B gene in sperm formation using Actl7b-deficient mice. Study: Actl7b deficiency leads to mislocalization of LC8 type dynein light chains and disruption of murine spermatogenesis. Image Credit: Komsan Loonprom / Shutterstock.com Background ACTL7B, a testis-specific actin-related protein (Arp), shares up to…
Vitrified before and after genome editing via electroporation
Background: Cryopreservation of bovine zygotes allows for a flexible schedule of genome editing via electroporation. However, vitrification-induced cell membrane damage may not only affect embryonic development but also genome mutation. Objective: To investigate the effects of vitrification of zygotes before and after electroporation treatments on the development and genome mutation…
Mutation in Polycomb repressive complex 2 gene OsFIE2 promotes asexual embryo formation in rice
CRISPR/Cas9-induced mutants at OsFIE1 and OsFIE2 Among ~150 T0 plants transformed with the CRISPR/Cas9 vector targeting the two FIE genes (Fig. 1a), we focused on plants with normal pollen dehiscence, as heterozygous fie mutants and other Polycomb mutants are not expected to give pollen sterility in heterozygous condition7,8,9,10,11,12,13,14. Four plants…
Significant decrease of maternal mitochondria carryover using optimized spindle-chromosomal complex transfer
. 2023 Oct 5;21(10):e3002313. doi: 10.1371/journal.pbio.3002313. eCollection 2023 Oct. Xiaoyu Liao 1 , Wenzhi Li 1 , Kaibo Lin 1 , Wei Jin 1 , Shaozhen Zhang 1 , Yao Wang 1 , Meng Ma 1 , Yating Xie 1 , Weina Yu 1 , Zhiguang Yan 1 , Hongyuan Gao 1 , Leiwen Zhao 1 , Jiqiang Si 1 , Yun Wang …
A G1528C Hadha knock-in mouse model recapitulates aspects of human clinical phenotypes for long-chain 3-hydroxyacyl-CoA dehydrogenase deficiency
All animal procedures were reviewed and approved by the OHSU IACUC (eIACUC #B11243). All experiments were performed in accordance with AAALAC and ARRIVE guidelines. Age, sex, and N of the mice for each experiment can be found in Supplementary Data 1. Mouse creation Mice were created through Cyagen Biosciences (Santa Clara,…
Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos
doi: 10.1038/s41467-023-40496-3. Ruimin Xu # 1 2 3 , Qianshu Zhu # 3 4 , Yuyan Zhao 1 , Mo Chen 2 5 , Lingyue Yang 1 2 , Shijun Shen 3 4 , Guang Yang 3 4 , Zhifei Shi 1 , Xiaolei Zhang 1 , Qi Shi 1 2 , Xiaochen Kou …
The “father” of the first transgenic children now wants to treat Alzheimer’s
Neither the stay in prison nor the unanimous condemnation of the scientific community did not remove the Chinese scientist from He Jiankui desire to continue their controversial research. Convicted in late 2018 for creating the world’s first genetically modified children, Dr. He was sentenced in 2019 to three years in…
Ablation of OCT4 function in cattle embryos by double electroporation of CRISPR-Cas for DNA and RNA targeting (CRISPR-DART)
Abstract CRISPR-Cas ribonucleoproteins are important tools for gene editing in pre-implantation embryos. However, the inefficient production of biallelic deletions in cattle zygotes has hindered mechanistic studies of gene function. In addition, the presence of maternal RNAs that support embryo development until embryonic genome activation may cause confounding phenotypes. Here, we…
Design Principles of a Novel Construct for HBB Gene-Editing and Investigation of Its Gene-Targeting Efficiency in HEK293 Cells
Jaing, T.-H., Chang, T.-Y., Chen, S.-H., Lin, C.-W., Wen, Y.-C., & Chiu, C.-C. (2021). Molecular genetics of β-thalassemia: A narrative review. Medicine, 100(45), e27522. Article CAS PubMed PubMed Central Google Scholar Rattananon, P., Anurathapan, U., Bhukhai, K., & Hongeng, S. (2021). The future of gene therapy for transfusion-dependent beta-thalassemia: The…
Electroporation-Mediated CRISPR/Cas9 Genome Editing in Rat Zygotes
doi: 10.1007/978-1-0716-2990-1_11. Affiliations Expand Affiliations 1 Animal Modeling Core, University of Missouri, Columbia, MO, USA. davisdaniel@missouri.edu. 2 Rat Resource and Research Center, University of Missouri, Columbia, MO, USA. 3 Animal Modeling Core, University of Missouri, Columbia, MO, USA. Item in Clipboard Daniel J Davis et al. Methods Mol Biol. 2023. Show…
Standard method for evaluating germline edits has large blind spots
Genetic/Congenital The intended use of gene editing tools on pre-implantation human embryos would be to avoid the development of congenital diseases in the upcoming baby. But it may have its own risks. Those risks were illustrated in a publication in the March 7, 2023, issue of Nature Communications, where researchers…
Epigenetic differences between male and female bovine blastocysts produced in vitro
Título: Autor: Bermejo Álvarez, Pablo; Rizos Dimitrios, Dimitrios; Rath, D.; Lonergan, P.; Gutiérrez Adán, Alfonso Palabras clave: mtDNAMethylationmRNA transcription Fecha de publicación: 2008 Editor: American Physiological Society Citación: Physiological Genomics 32(2): 264-272 (2008) Resumen: Epigenetic differences between male and female bovine blastocysts provide a plausible link between physiological and gene…
What Is Gene Editing and Why It Is Very Important to Twentyfirst Century?
by Prof Riaz A Shah Genome editing in livestock has the potential to bring about significant improvements in productivity, health, and welfare, but there are still challenges that need to be addressed. A SKUAST-K scientist at work The livestock industry is facing a growing demand for animal-based foods to feed…
A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes
Animals B6N mice, ICR (CD1) mice, SD rats, and LE rats were obtained from The Jackson Laboratory Japan (Yokohama, Japan). LH rats (No. 0873) were obtained from the National Bio Resource Project for the Rat (NBRP-Rat, Kyoto, Japan). ELKS conditional KO mouse was made floxed ELKS mouse crossed with E3N-Cre…
Seven technologies to watch in 2023
From protein sequencing to electron microscopy, and from archaeology to astronomy, here are seven technologies that are likely to shake up science in the year ahead. Single-molecule protein sequencing The proteome represents the complete set of proteins made by a cell or organism, and can be deeply informative about health…
Dynamics of DNA hydroxymethylation and methylation during mouse embryonic and germline development
Gu, T. P. et al. The role of Tet3 DNA dioxygenase in epigenetic reprogramming by oocytes. Nature 477, 606–610 (2011). Article CAS Google Scholar Wossidlo, M. et al. 5-Hydroxymethylcytosine in the mammalian zygote is linked with epigenetic reprogramming. Nat. Commun. 2, 241 (2011). Article Google Scholar Iqbal, K., Jin, S….
Versatile and efficient genome editing with Neisseria cinerea Cas9
PAM identification of NcCas9 by PAM-DOSE The type II-C Cas9 orthologue NcCas9 is of small size (1082 aa) and is closely related to conventional Neisseria meningitidis Cas9 (NmeCas9)11 (Fig. 1a). The NcCas9 is 94% identical to Nme1Cas9, and the divergences lie mainly in the C-terminal PAM-interacting domain (PID) (Fig. 1a and S1)….
C698R mutation in Lrsam1 gene impairs nerve regeneration in a CMT2P mouse model
Animal generation The Lrsam1C698R knock-in mice were generated on a C57Bl/6J background using the CRISPR/Cas9 technique. Briefly, single-cell zygotes from C57BL/6J mice were microinjected with mRNA encoding Cas9 and a guide sequence (5′-ACAGCAGCAGACGTGGCCAC-3′ at 20 ng/µl) to target the exon 26 of Lrsam1. A single stranded DNA oligo carrying the C698R mutation…
Cristae-dependent quality control of the mitochondrial genome
S. cerevisiae cells distinguish between WT and mutant mtDNA. (A) Schematic illustration of the pedigree analysis. Two Δarg8 yeast strains harboring WT or Δcob::ARG8 mtDNA were mated. Zygotes were isolated, and daughter cells from up to five consecutive generations were separated and placed on free spots on the agar plate…
2020 Heritable Human Genome Editing – Genômica
editing methodologies. It is possible that continuing research may yield new methodologies that rapidly supersede the safety and efficacy of current editing approaches. Non-Heritable Genome Editing: The Use of Genome Editing in Somatic Cells One potential alternative to HHGE for the treatment of genetic dis- eases is somatic genome…
Validation of gene editing efficiency with CRISPR-Cas9 system directly in rat zygotes using electroporation mediated delivery and embryo culture
doi: 10.1016/j.mex.2021.101419. eCollection 2021. Affiliations Expand Affiliations 1 Department of Biology, University of Alabama at Birmingham, Birmingham, AL, USA. 2 Cystic Fibrosis Research Center, Division of Pulmonary, Allergy, and Critical Care Medicine, University of Alabama at Birmingham, Birmingham, AL, USA. Free PMC article Item in Clipboard Anil K Challa et al….